LOCUS       NZ_CP020555            10405 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. Sge12 chromosome, complete genome.
ACCESSION   NZ_CP020555
VERSION     NZ_CP020555.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Sge12
  ORGANISM  Streptomyces sp. Sge12
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7983613)
  AUTHORS   Tao,M., Xu,J., Xu,M., Liu,K., Zhao,Z. and Peng,Q.
  TITLE     Genome sequence of Streptomyces sp. Sge12, producing unknown
            compounds against rice blast disease
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7983613)
  AUTHORS   Tao,M., Xu,J., Xu,M., Liu,K., Zhao,Z. and Peng,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, Huashan Road 1954, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: RS_HGAP Assembly v. 3; BWA v. 0.7.10; Bowtie v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.0x
            Sequencing Technology :: Illumina HiSeq 4000; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,312
            CDSs (total)                      :: 7,216
            Genes (coding)                    :: 7,106
            CDSs (with protein)               :: 7,106
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 32 of 111
            Pseudo Genes (incomplete)         :: 91 of 111
            Pseudo Genes (internal stop)      :: 8 of 111
            Pseudo Genes (multiple problems)  :: 18 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:35:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7464519
            Orig. end                         :: 7474924
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020555.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="37"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(203..217)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            489..2084
                     /locus_tag="B6R96_RS33435"
                     /old_locus_tag="B6R96_33435"
     CDS             489..2084
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037768.1"
                     /locus_tag="B6R96_RS33435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33435"
                     /product="hypothetical protein"
                     /protein_id="WP_081524579.1"
                     /transl_table=11
                     /translation="MPSSRIRRAWLSAAAAAALAFTGLAGPATAKDLPPDVPGSWIQEP
                     RATEGAPSAFGARAAAVPGGPASPGDFTLSPGEMNAETTQRVARLASVLPESGVQDLLA
                     AANRPIEPGCSRDPFAAAPDPAVKYCLKDDDSVSREWVPQGFTGVSDAKDNELWGAAGK
                     IQLFASYDGWDPGRESDPNPAAGDCTAAELEANDACNQKGVRITFVQSRNNPATGSPEI
                     KYRHVLLGWTYVNSADHVSFDGLHAAEYPIQKGVHAGGIVWYGNYLYVADTRGGLRVFD
                     MRFIMDLDPDGDPATHDRMGTDADGVKTTADVEDKTKVGRHDNVWYSFGYRYVMPQVAA
                     WKFKATQSNPKGSFACVSTGAPKASYISLDRSTGPDRLIMGEYCRPESGYPSTGRIASY
                     PVAALEQRSADVTAQGWANYLPLANGGAQGAAAYNGTLYVNESKGTDEPGNLWRYLWSD
                     GRLVQDGQAVKTARGAEDLYVERGTGRLWSLSEHRPGSADDCRANPGPGDPAGADFCQR
                     VLYAHELSWLDSQP"
     gene            complement(2138..2689)
                     /locus_tag="B6R96_RS33440"
                     /old_locus_tag="B6R96_33440"
     CDS             complement(2138..2689)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995990.1"
                     /locus_tag="B6R96_RS33440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33440"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_081524580.1"
                     /transl_table=11
                     /translation="MSEQRRPADPSPAADDIDEVTHAVLAAAQRLASIAARALAAAGDG
                     GRGGHGVTLAQFRMLEVLVARGHVKLVALAGLMAVNPSTAMRMIDRLIAAGLADRRPNR
                     ENRRETVLTATESGLRLVGEVTARRRAETAAALARLRPAERTVLIGALGAFAATGPQPA
                     GDGPHAIPDLLGCVQPYALG"
     misc_feature    2808..2828
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.54"
                     /tool="antismash"
     misc_feature    2828..2845
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.88"
                     /tool="antismash"
     misc_feature    complement(2842..2855)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.7"
                     /tool="antismash"
     misc_feature    2844..2858
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.27"
                     /tool="antismash"
     gene            3011..3571
                     /gene="ectA"
                     /locus_tag="B6R96_RS33445"
                     /old_locus_tag="B6R96_33445"
     CDS             3011..3571
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337972.1"
                     /locus_tag="B6R96_RS33445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33445"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_030387830.1"
                     /transl_table=11
                     /translation="MTGAHADLATRPRTPAPTTSPSGIRADRPDLCDGPDLWRIARDAK
                     TLDVNSPYSYLLWCRDFAATSAVARDGDGRPVGFVTGYLRPDTPATLLIWQVAVDADAR
                     GLGIAGHLLDHLTARVAAERPLDALETTISPGNTASERLFASFAERHGAQLAREVLFAQ
                     EHFPGGAGHHDAEVLHRIAPLAL"
     gene            3681..4949
                     /gene="ectB"
                     /locus_tag="B6R96_RS33450"
                     /old_locus_tag="B6R96_33450"
     CDS             3681..4949
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 1.1e-71.
                     Score: 233.6. Matches aSDomain:
                     nrpspksdomains_B6R96_RS33450_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="B6R96_RS33450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33450"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_081524581.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.8e-78, bitscore:
                     261.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTAPNPTVFDTVESEVRSYCRAWPVVFDRAEGSRLHDENGRSY
                     LDFFAGAGSLNYGHNNPVLKQALLDYIARDGITHALDMSTTAKRAFLETFHSTVLAPRG
                     LPYKVMFPGPTGTNAVEAALKLARKAKGREAVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHSTPMPFDNYLGGRVPDFLWFERLLEDSGSGLNDPAAVIVETVQGEGGVNVARA
                     EWLRALADLCRRRDMLLIVDDVQMGCGRTGDFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALQTYWRDGVLRERTRVQGARIEQALV
                     DLCADHERDGASYRGRGMVWGLEFTRKERAAEVCHRAFEHGLLVETSGPESEVVKLLPP
                     LTVSADELDEGLDILARSVRETA"
     aSDomain        3765..4775
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B6R96_RS33450_Aminotran_3.1"
                     /evalue="1.10E-71"
                     /label="B6R96_RS33450_Aminotran_3.1"
                     /locus_tag="B6R96_RS33450"
                     /protein_end="365"
                     /protein_start="28"
                     /score="233.6"
                     /tool="antismash"
                     /translation="FDRAEGSRLHDENGRSYLDFFAGAGSLNYGHNNPVLKQALLDYIA
                     RDGITHALDMSTTAKRAFLETFHSTVLAPRGLPYKVMFPGPTGTNAVEAALKLARKAKG
                     REAVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHSTPMPFDNYLGGRVPDFLWFER
                     LLEDSGSGLNDPAAVIVETVQGEGGVNVARAEWLRALADLCRRRDMLLIVDDVQMGCGR
                     TGDFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATAALQTYWRDGVLRERTRVQGARIEQALVDLCADHERDGASYRGRGMVWGLEFT"
     gene            5001..5405
                     /locus_tag="B6R96_RS33455"
                     /old_locus_tag="B6R96_33455"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="B6R96_RS33455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33455"
                     /product="ectoine synthase"
                     /protein_id="WP_030387828.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.5e-52, bitscore:
                     173.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFQDIEGTDRHVRAASGTWESKRLILAKERVGFSLHETVLY
                     AGTETSMWYANHVEAVVCVEGEAELTDEETGEKHTITPGTVYVLDGHERHTLRVVREFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPDPV"
     gene            5455..6357
                     /gene="thpD"
                     /locus_tag="B6R96_RS33460"
                     /old_locus_tag="B6R96_33460"
     CDS             5455..6357
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004613.1"
                     /locus_tag="B6R96_RS33460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33460"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_081524582.1"
                     /sec_met_domain="PhyH (E-value: 4.5e-38, bitscore: 130.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVSERTLADLYPTRGSEEVLIGRKDPVVWSEPGTPGPFWAREL
                     EDFERDGFLAAEQLIGPEEVEEYRAEMDRLVSDPAVRADERAIVEPGSGEVRSVFEVHR
                     ISEVFARLAADPRIADRARQILGSDVYVHQSRINAKPGFGASGFYWHSDFETWHAEDGL
                     PRMRTVSVSIALTPNHTTNGSLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPTPE
                     ALTRFATACGIRQFTGAAGSATWFDCNAMHGSGDNITPYPRSNVFLVFNSVDNLPAEPF
                     AAPIRRPSFIASRDFTPLP"
     gene            6464..7957
                     /locus_tag="B6R96_RS33465"
                     /old_locus_tag="B6R96_33465"
     CDS             6464..7957
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309675.1"
                     /locus_tag="B6R96_RS33465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33465"
                     /product="MFS transporter"
                     /protein_id="WP_081524583.1"
                     /transl_table=11
                     /translation="MNLGRTKNETEPGAPPRQGAALAALAAAQFAVMLATSIVNVALPQ
                     IRSGVGLSDGGTTWVVNAYGLAFGALLLAGGRAGDLLGRRRVLISGLAVFAAASLAAGL
                     AGSPGLLIAARALQGIGAAAIAPAALALAMDLFPPGPGRGRVLGVWGAVSGAGGAGGVL
                     LGGVLTQAWGWPWLFHAVALGTTLVLIAVATLVPRAGTRGSGRFDLLGTATVTLALTCL
                     VWALTSARAQGWTDPGVLAALLGAAALLVVFVLVERRRPDALVPARLFTAGRVAAGNLL
                     MALLGSVWIALFFFLPLYQQQVLGMSPLATGAGQLPLAVANMLGAILAPRIAARIGTTA
                     TLITGLLAEAAGLLWLSRMRADGSYLVDALGPSVLVGLGLSLAFVQLTALAVDGVPRQD
                     TGLAGGLVNTTRQVGGAIGLAALATLAGSVTAHAATHQPHLEALTAGYRAAFAVSAAVL
                     AATALLAVPLTRPLRRSRRAVPEATPSPQGRATEHAPAA"
     gene            8034..8477
                     /locus_tag="B6R96_RS33470"
                     /old_locus_tag="B6R96_33470"
     CDS             8034..8477
                     /NRPS_PKS="Domain: Polyketide_cyc2 (11-144). E-value:
                     1.6e-22. Score: 72.4. Matches aSDomain:
                     nrpspksdomains_B6R96_RS33470_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /locus_tag="B6R96_RS33470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33470"
                     /product="SRPBCC family protein"
                     /protein_id="WP_081524584.1"
                     /transl_table=11
                     /translation="MHTTIDTTAPVAVRLSTVIDASPATVWELHTDIDDWAAWNDGVDR
                     ARLNGPLEVGSSFTWLTHGLEITSTVLELVPGQRIVWGGTVDGIVGIHVWTFEESGAGV
                     TVRTEESWSGDPVDAAVDELTAALRASLQSWLDSLKARAEQPA"
     aSDomain        8067..8465
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B6R96_RS33470_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="1.60E-22"
                     /label="B6R96_RS33470_Polyketide_cyc2.1"
                     /locus_tag="B6R96_RS33470"
                     /protein_end="144"
                     /protein_start="11"
                     /score="72.4"
                     /tool="antismash"
                     /translation="AVRLSTVIDASPATVWELHTDIDDWAAWNDGVDRARLNGPLEVGS
                     SFTWLTHGLEITSTVLELVPGQRIVWGGTVDGIVGIHVWTFEESGAGVTVRTEESWSGD
                     PVDAAVDELTAALRASLQSWLDSLKARAE"
     gene            8527..9132
                     /locus_tag="B6R96_RS33475"
                     /old_locus_tag="B6R96_33475"
     CDS             8527..9132
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061141.1"
                     /locus_tag="B6R96_RS33475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33475"
                     /product="ABC transporter permease"
                     /protein_id="WP_081525375.1"
                     /transl_table=11
                     /translation="MSSHTTLSPPVVRDHPTRRPREVDNRAAYVSFGLAYVLGHGAAAA
                     SHGSAPVLDLPGWLPMALLGLGLVTGVVLATLAALRAQHGAGAPEILSGKLLGAAWIIG
                     FTALFLAVTGLTSVLDHPELQSVLWPAGSGLVVGLLYLAEGAVRRNVLHYSLGVWLAIT
                     STAALALAAPGFYWVLALGGGGAYALAAALEHRRLHAR"
     gene            complement(9139..9744)
                     /locus_tag="B6R96_RS33480"
                     /old_locus_tag="B6R96_33480"
     CDS             complement(9139..9744)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735601.1"
                     /locus_tag="B6R96_RS33480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_33480"
                     /product="hypothetical protein"
                     /protein_id="WP_081524585.1"
                     /transl_table=11
                     /translation="MRLYAQTPARRNRQVLADLIAVGLMAAAVWFALAVHDAIMMLAEP
                     GRRVESSGDSLAGGLADAGDAASNVPFVGDLLKKPLQSAADASSGFADAGQSMQDAVGQ
                     LATLTTVALIVLPVAVVLLLWLPARLRWIRRSITLRRLFDAPGGADLLALRALTGPLGD
                     LLSLPTPPGGLADAWRRGDERVIGELSEAALRRAGLRP"
     misc_feature    complement(10215..10229)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.93"
                     /tool="antismash"
ORIGIN
        1 aagccctccc cgctccagga cctggccgcg ctgccccggt acgcggctgt cttcggcctc
       61 cgcgggatgc cgcgggccgg ggaggtccag aacgccatgc acgaggtcca tccgcggaca
      121 ccgcactggt acctgccctc cgtggggacg gaccggggac ttcaggggag gggggtcggc
      181 tcggcgctgc tgcgccagca gttggccgac tgcgaccggc tcggacaacc cgcctacctg
      241 gagtccagcg cgcccgccaa cgtccccttc tacgaacggc tgggcttccg cgtcaccggt
      301 gagatccggc tcccgggcgg cggaccgacg ctgtggccca tgtggcgcga tccccaccgc
      361 tgacgggggt cttcaaccgg cgtgctcggc ctggtcattg acgagtcgat cacccttgtg
      421 ttccgcatac gtgttctgga agcctccgct tgactcacct gtcacacgag caacggagca
      481 acccccgcat gccctcaagt cgaatacgtc gcgcctggct ctcggccgcc gccgcggcag
      541 cgctcgcgtt caccggtctc gcaggaccgg cgacggcgaa ggaccttccg cccgatgtgc
      601 cggggtcctg gatccaggag ccccgcgcga ccgaaggcgc cccctcggcc ttcggtgccc
      661 gcgctgccgc cgtccccggc ggccccgcct cgcccggcga tttcacgctg tcgccgggcg
      721 agatgaacgc cgagaccacc cagagggtcg cccgcctggc ctcggtgctg cccgagtcgg
      781 gcgtacagga cctgctggcc gcggcgaacc gtcccatcga gccggggtgt tcccgcgacc
      841 cgttcgccgc cgcccccgac ccggccgtca agtactgcct gaaggacgac gactccgtct
      901 ccagggagtg ggtcccgcag ggtttcaccg gcgtctccga cgccaaggac aacgagctct
      961 ggggcgcggc cgggaagatc cagctgttcg cctcctacga cggctgggac ccgggccgcg
     1021 agagcgaccc gaacccggcg gccggcgact gcaccgccgc cgagctggag gccaatgacg
     1081 cctgcaacca gaagggcgtc cggatcacct tcgtgcagag ccggaacaac ccggccaccg
     1141 gcagccccga gatcaagtac cggcacgtgc tgctgggctg gacctatgtg aactccgcgg
     1201 accacgtctc cttcgacggc ctgcacgccg ccgagtaccc gatccagaag ggcgtccacg
     1261 ccggtgggat cgtctggtac ggcaactacc tctacgtcgc ggacacgcgg ggcggcctgc
     1321 gcgtcttcga catgcgcttc atcatggacc tcgacccgga cggcgacccc gccacgcacg
     1381 accggatggg caccgacgcc gacggggtga agaccaccgc cgacgtcgag gacaagacga
     1441 aggtcggccg gcacgacaac gtctggtaca gcttcggcta ccgctacgtc atgccgcagg
     1501 tggcggcgtg gaagttcaag gcgacgcaga gcaaccccaa gggctccttc gcctgcgtct
     1561 ccaccggcgc cccgaaggcg tcctacatct ccctggaccg cagtacgggt ccggaccgcc
     1621 tgatcatggg cgagtactgc cgtccggaga gcggctaccc ctccacgggc cgcatcgcct
     1681 cctacccggt cgcggccctg gagcaacgct ccgcggacgt gaccgcgcag ggctgggcca
     1741 actacctgcc cctggccaac ggcggcgccc agggcgcggc ggcgtacaac ggcacgctgt
     1801 acgtgaacga gtccaagggg acggacgagc cgggcaacct gtggcgctac ctctggagtg
     1861 acggccggct ggtccaggac ggtcaggccg tcaagaccgc ccggggcgcc gaggacctct
     1921 acgtcgagcg cggcacggga cgcctgtggt ccctctccga acaccgtccg ggctcggcgg
     1981 acgactgccg ggcgaacccg ggccccggtg atcccgcggg cgcggacttc tgccagcggg
     2041 tgctctacgc acacgagttg agctggctcg acagccagcc gtagtcccgg ccgcagccgc
     2101 ggccccccgc cccggccgca gccgggggcg gcccggctca gcccagggcg tacggctgca
     2161 cgcaccccag gaggtccggg atggcgtggg gtccgtcccc ggcgggctgc gggcccgtgg
     2221 cggcgaacgc gccgagcgcg ccgatcagta cggtgcgttc ggcgggccgg agccgggcga
     2281 gggcggcggc cgtctccgcc cggcgccggg ccgtcacctc gcccaccagc cgcaggccgg
     2341 actccgtggc ggtgagcacc gtctcccgcc ggttctcccg gttgggcctg cggtccgcca
     2401 ggcccgcggc gatcagccgg tcgatcatgc gcatggcggt cgaggggttg accgccatca
     2461 gccctgcgag ggcgaccagt ttcacgtgcc cgcgcgccac caggacctcg agcatccgga
     2521 actgggcgag cgtgaccccg tggccgccgc ggcccccgtc cccggcggcc gccagggcgc
     2581 gggccgcgat ggacgcgagc cgttgggcgg cggccagcac cgcgtgcgtc acctcgtcga
     2641 tgtcgtccgc ggcgggtgac ggatccgcgg gtcgccgttg ctcgctcata cccctttgta
     2701 ccgcccggcc gtccgggaca cacgacgggc cgaaagggcc cactcgcctt tcccgttatc
     2761 cggagccgaa acggggggac tcgcgaatgt cgtggctttc acaccgcaaa tatggcacag
     2821 ggcaaagacg ttacagtctc gttatctttc agaattttgg gatggcttgt ccggataaat
     2881 agcggttttc ttcgtggggg agaacgggcc aggaccaccc cggcgccgct gccggataac
     2941 acggacgcag cgtgtacgga accccgttcg ggcatgtctt ttcagccgat gatcggtagc
     3001 ttcggcccgc atgaccggtg ctcatgcaga cctggccacc cgcccccgaa cccccgcccc
     3061 cacgacgagt ccctccggaa tccgggccga ccgccccgac ctgtgcgacg gccccgacct
     3121 ctggcgcatc gcccgcgacg cgaagacgct ggacgtcaac tccccctaca gctacctgct
     3181 ctggtgccgt gacttcgccg ccacctccgc cgtcgcccgc gacggcgacg gccggcccgt
     3241 cggcttcgtc accgggtacc tgcgccccga cacccccgcc accctgctga tctggcaggt
     3301 cgccgtcgac gccgacgccc gcgggctggg gatcgccggg caccttctcg accacctcac
     3361 cgcacgggtc gccgccgagc gcccgctcga cgcgctcgag accaccatca gcccgggcaa
     3421 caccgcctcg gaacggctgt tcgcgtcctt cgccgaacgc cacggtgccc aactcgcccg
     3481 ggaggtcctc ttcgcgcagg agcactttcc cggcggcgcc ggacaccacg acgccgaggt
     3541 gctgcaccgc atcgcacccc tggccctctg acaccggcca ccaccccggc caccggccac
     3601 cacccccggc caccgaccac cacgtccatc accgtccgcg accgtccgcc ggcggacacc
     3661 gcaggagagg caggtccccc ttgaccacga cagcaccgaa cccgaccgtc ttcgacaccg
     3721 tcgagtcgga ggtacgcagc tactgccgcg cctggccggt cgtcttcgac cgcgcggagg
     3781 gcagccgcct ccacgacgag aacggccgct cctacctgga cttcttcgcg ggcgccggct
     3841 ccctcaacta tgggcacaac aaccccgtcc tcaaacaggc actgctcgac tacatcgcgc
     3901 gcgacggcat cacgcacgcc ctggacatgt cgacgaccgc caagcgggcc ttcctggaga
     3961 ccttccactc caccgtcctc gccccccgcg gactgccgta caaggtgatg ttcccgggcc
     4021 ccaccggcac caacgccgtc gaggcggcgc tcaagctggc gcgcaaggcc aaggggcgcg
     4081 aggccgtcgt ctccttcacc aacgccttcc acggcatgtc cctgggctcg ctggccgtca
     4141 ccggcaacgc cttcaagcgc gcgggcgccg gcataccgct ggtccacagc acgcccatgc
     4201 ccttcgacaa ctacctcggc ggccgcgtcc cggactttct ctggttcgag cggctcctgg
     4261 aggacagcgg atccggcctc aacgacccgg ccgccgtgat tgttgagacc gtgcagggcg
     4321 agggcggcgt caacgtggcg cgcgcggagt ggctgcgcgc cctcgccgac ctgtgccgcc
     4381 gccgggacat gctgctgatc gtcgacgacg tccagatggg ctgcggacgg accggcgact
     4441 tcttctcctt cgaggaagcg ggcatcacac ccgacatcgt caccgtctcc aagtccatca
     4501 gcggctacgg actgcccatg tcgctctgcc tgttcaagcc cgagctcgac atctgggagc
     4561 ccggcgagca caacggcacg ttccgcggca acaaccccgc cttcgtcacg gcgaccgccg
     4621 cactgcagac ctactggcgc gacggcgtcc tgcgcgaacg cacccgtgtc cagggcgccc
     4681 gcatcgagca ggcgctcgtc gacctctgcg cggaccacga gcgggacggc gcctcgtacc
     4741 gcggccgcgg catggtgtgg ggcctcgagt tcacccgcaa ggagcgcgcc gccgaggtct
     4801 gccaccgggc gttcgagcac ggcctgctcg tcgagacctc cgggccggag agcgaggtgg
     4861 tcaagctgct gccgccgctc accgtgtcgg cggacgagct cgacgagggc ctcgacatcc
     4921 tcgcgcggtc cgtccgcgag acggcctgac ccgccgcccc caccacccca cccgccgcca
     4981 ccacgaaagg cagcaccacc atgatcgtcc gatccttcca ggacatcgaa ggcaccgacc
     5041 gccacgtcag ggcggcgtcg ggcacctggg agagcaagcg cctcatcctg gcgaaggaac
     5101 gcgtcggctt ctccctccac gaaaccgtcc tgtacgccgg taccgagacg tccatgtggt
     5161 acgcgaacca cgtcgaggcg gtcgtgtgcg tcgagggcga ggccgaactc accgacgagg
     5221 agaccggcga gaagcacacc atcacgcccg gcaccgtcta cgtcctcgac ggccacgagc
     5281 gccacaccct gcgcgtcgtc cgcgagttcc gctgcctgtg cgtcttcaac ccgcccgtca
     5341 ccggccgcga ggaccacgac gagaacggcg tgtacccgct gctcaccgag ccggacccgg
     5401 tctgacccac cccgcaggaa cccaccgacc ccgagaggag aggcaagcac caccatgacc
     5461 actgtctccg aacgcaccct cgccgacctg tacccgaccc ggggctccga agaagtcctc
     5521 atcggccgca aggaccccgt cgtgtggtcc gaacccggca cccccggacc gttctgggcg
     5581 cgcgaactgg aggacttcga acgcgacggc ttccttgccg ccgagcagct gatcggcccg
     5641 gaagaggtcg aggagtaccg ggccgagatg gaccggctgg tgagcgaccc cgccgtgcgg
     5701 gccgacgaac gggccatcgt ggaaccggga tcgggcgagg tccgctcggt cttcgaggtc
     5761 caccgcatca gcgaggtctt cgcgcgcctc gccgccgatc cgcggatcgc ggaccgcgcc
     5821 cggcagatcc tcggttccga cgtgtacgtc caccagtccc gcatcaacgc caagcccggc
     5881 ttcggcgcga gcggcttcta ctggcactcc gacttcgaga cctggcacgc cgaggacggc
     5941 ctgccccgta tgcggacggt atcggtgtcc atcgccctca cgccgaacca cacgaccaac
     6001 ggcagcctga tgatcatgcc gggttcgcac cgcacgttcc tcggctgcgc gggcgcgacg
     6061 ccgaaggaca actacaagaa gtcgctgcag atgcaggacg ccggaacgcc cacccccgag
     6121 gcgctcaccc ggttcgcaac cgcctgcggc atacggcagt tcaccggggc cgccggatcg
     6181 gccacctggt tcgactgcaa cgcgatgcac ggttccgggg acaacatcac cccctacccg
     6241 cgcagcaacg tcttcctggt gttcaacagc gtggacaacc tgcccgcgga gcccttcgcg
     6301 gccccgatcc ggcgcccgtc cttcatcgcc tcgcgcgact tcacgccgct gccctgaggc
     6361 acgcacccgg caaggccgag tggacagggg cggacacgac tggacagtga ccgcccctgt
     6421 cgggcatgga ttgtggacgc cgcccgacac gaggctgtgc gccatgaacc tcggacgcac
     6481 gaagaacgaa accgaacccg gtgccccacc ccgccagggg gccgcactcg ccgccctggc
     6541 cgcagcgcag ttcgccgtga tgctggccac ctccatcgtg aacgttgcgc tgccacagat
     6601 ccgctccgga gtgggcctgt cggacggcgg caccacctgg gtggtcaacg cctacggcct
     6661 ggcgttcggc gcactgctcc tcgccggcgg ccgggcgggc gacctgctcg gccgccgccg
     6721 ggtgctgatc tccggcctcg ccgtgttcgc ggcagcctcg ctggcggcgg gactggccgg
     6781 ctcgccgggc ctgctgatcg ccgcccgcgc cctccaaggc atcggcgccg ccgccatcgc
     6841 acccgccgcg ctcgccctgg cgatggacct gttcccgccc ggccccggca ggggccgggt
     6901 cctcggagtg tggggcgccg tctccggcgc gggcggcgcg ggcggcgtcc tgctgggcgg
     6961 cgtactcacc caggcatggg gctggccctg gctcttccac gccgtcgcgc tcggcacgac
     7021 gctggtcctc atcgccgtgg cgacgctcgt accccgcgcc ggcacccgcg gctccggacg
     7081 gttcgacctg ctgggcaccg cgaccgtcac cctcgccctg acctgcctgg tgtgggcact
     7141 gaccagcgcc cgcgcccagg gctggaccga ccccggagtg ctggccgccc tcctgggcgc
     7201 cgccgcgctg ctcgtggtct tcgtcctcgt cgaacgccgc cgacccgacg ccctggtccc
     7261 ggcccggctg ttcacggccg gccgggtcgc cgcgggcaat ctcctgatgg cgctgctggg
     7321 atccgtatgg atcgcgctgt tcttcttcct cccgctctac cagcaacagg tcctcggcat
     7381 gagcccgctg gccaccgggg cgggccaact ccccctcgcc gtcgccaaca tgctcggcgc
     7441 gatcctcgcg ccccgcatcg ccgcccgcat cggcaccacg gccaccctga tcacgggcct
     7501 gctcgccgag gccgcggggc tgctgtggct gtcacggatg agagcggacg ggagctacct
     7561 cgtcgacgcc ctcggaccga gcgtcctggt cggcctcggc ctgagcctcg ctttcgtcca
     7621 gctcaccgcg ctcgccgtgg acggcgtacc gcggcaggac accggcctgg ccggcggact
     7681 ggtcaacacc acgcgccagg tcggcggcgc gatcgggctc gctgccctcg ccaccctcgc
     7741 cggctccgtc accgcccatg cggccaccca tcagccccac ctggaagccc tcaccgcggg
     7801 ctaccgggcc gccttcgcgg tctcggccgc ggtcctggcc gccaccgcgc tcctcgcggt
     7861 gccgctgacc cgcccgctcc gccgctcccg ccgtgccgtc ccggaggcca cgccctcccc
     7921 gcagggccgg gcgaccgaac acgcacccgc cgcctgatcc acgtctgatc cacagcccat
     7981 ccctccggga acccgatgat ccggcgaccc ggccacccga agaagactca tccatgcaca
     8041 ccaccatcga caccacggcc cccgtcgccg tccgcctctc gacggtgatc gacgcctctc
     8101 ccgcgaccgt ctgggagctg cacaccgaca tcgacgactg ggcggcctgg aacgacggcg
     8161 tcgaccgggc ccggctgaac ggcccgctgg aggtcggcag cagcttcacc tggctcacgc
     8221 acggcctgga gatcacctcc accgtcctcg aactggtccc cggacagcgg atcgtctggg
     8281 gcgggaccgt cgacggcatc gtcggcatcc acgtgtggac cttcgaggag tccggggccg
     8341 gcgtcaccgt ccgcaccgag gagtcctgga gcggggaccc cgtcgacgcc gcggtcgacg
     8401 agctcaccgc cgccctgcgg gcctccctgc agagctggct cgacagcctc aaggcacgcg
     8461 ccgaacagcc cgcctgaccg tcaccccgcc cgccccaccc cccacccccc gatggaggtc
     8521 tccaccgtgt ccagccacac caccctctcg ccgcccgtcg tccgtgacca ccccacccgc
     8581 cgcccccgcg aggtcgacaa ccgcgcggcc tacgtcagct tcggactcgc ctacgtcctc
     8641 ggccacggcg cggccgccgc ctcccacgga tccgcgcccg tgctcgacct gcccggctgg
     8701 ctgcccatgg cactcctcgg cctcgggctc gtgaccggcg tcgtcctggc cacgctcgcc
     8761 gccctacggg cccagcacgg ggccggcgca ccggagatcc tctccggaaa gctgctcggc
     8821 gccgcctgga tcatcgggtt caccgcactg ttcctcgccg tcaccggcct gacgtccgta
     8881 ctcgaccacc cggaactcca gtccgtcctg tggccggccg gctccggact cgtcgtcggc
     8941 ctgctctacc tcgccgaggg cgccgtacgc cgcaacgtgc tgcactacag cctcggcgtc
     9001 tggctcgcca tcacctccac cgcggcactc gcgctcgcgg cccccgggtt ctactgggtc
     9061 ctcgccctcg ggggcggcgg cgcctacgcc ctcgccgcgg ccctcgaaca ccgccgcctc
     9121 cacgcccgct gacggccgtc agggccgcag gccggcccgc cgcagggcgg cctccgagag
     9181 ctcgccgatg acgcgctcgt ccccgcgccg ccaggcgtcg gcgaggcctc ccggcggggt
     9241 ggggagcgag aggaggtcgc ccagcggtcc ggtcagggcg cgcagggcga gcaggtcggc
     9301 gccgcccggt gcgtcgaaca ggcgccgcag ggtgatgctg cgccggatcc agcgcaggcg
     9361 cgcggggagc cacaacagca ggacgacggc caccggcagg acgatcaggg ccaccgtggt
     9421 cagcgtggcc agctggccga cggcgtcctg catcgactgg ccggcgtccg cgaacccgct
     9481 actggcgtcg gccgcggact ggagcggctt cttcaggagg tcgccgacga acgggacgtt
     9541 cgaggccgcg tcgccggcgt cggcgagtcc gccggcgagg ctgtcgccgg aactctccac
     9601 cctgcggccc ggctcggcca acatcatgat cgcgtcgtgc acggccaggg cgaaccacac
     9661 cgcggcggcc atcaggccca cggcgatcag gtccgcgagc acctggcggt tccgacgtgc
     9721 tggggtctgt gcgtagaggc gcatcggcgg gctcccgttc gagtcaggac gtcgaggacg
     9781 tacggacccc accggacggg cccggccggc cggtcgaccg tacgccggtc tctacccttc
     9841 caccggcccc ggccggccaa ccgcccaccg ccggaaccgg acgagccggc cggtcaccga
     9901 ccggccggcc cgtcgaaccg gcctaggccg tctcttccgg atcttgtcgg gcccggcccg
     9961 cccggcacgg cacctcgccg cgttgtcggg gcgcccgagt acgtccagta cacgggcgcc
    10021 cctccgcctt gcgatgtacc gcaccggacg acccgggctg atccgacaag atccgaaaga
    10081 gacggcctag gcgaggtcga accggtcgag gttcatcacc ttgtcccagg cggagacgaa
    10141 gtcacggacg aacttctctc ccgcgtcctt cgacgcgtag acctcgcaga gcgcccggag
    10201 ctgggcgtgc gaaccgaaga cgaggtccac cgcggtcgcc gtccacttga cgtcgcccgt
    10261 ggcgtggtcc cggccctcga acacgttctc cgccgactcc gacgccttcc actgcgtccc
    10321 catgtcgagc aggttgacga agaagtcgtt ggtcagcgcc tccgggcggt ccgtgaacag
    10381 cccgtgcggg gactgccgga agccg
//
