LOCUS       NZ_CP020555            10972 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. Sge12 chromosome, complete genome.
ACCESSION   NZ_CP020555
VERSION     NZ_CP020555.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Sge12
  ORGANISM  Streptomyces sp. Sge12
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7983613)
  AUTHORS   Tao,M., Xu,J., Xu,M., Liu,K., Zhao,Z. and Peng,Q.
  TITLE     Genome sequence of Streptomyces sp. Sge12, producing unknown
            compounds against rice blast disease
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7983613)
  AUTHORS   Tao,M., Xu,J., Xu,M., Liu,K., Zhao,Z. and Peng,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, Huashan Road 1954, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: RS_HGAP Assembly v. 3; BWA v. 0.7.10; Bowtie v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.0x
            Sequencing Technology :: Illumina HiSeq 4000; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,312
            CDSs (total)                      :: 7,216
            Genes (coding)                    :: 7,106
            CDSs (with protein)               :: 7,106
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 32 of 111
            Pseudo Genes (incomplete)         :: 91 of 111
            Pseudo Genes (internal stop)      :: 8 of 111
            Pseudo Genes (multiple problems)  :: 18 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:35:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6753533
            Orig. end                         :: 6764505
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020555.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10972
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="33"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10972
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10972
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5972](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5972)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            1305..2129
                     /locus_tag="B6R96_RS30355"
                     /old_locus_tag="B6R96_30355"
     CDS             1305..2129
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049992.1"
                     /locus_tag="B6R96_RS30355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_30355"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_081524189.1"
                     /transl_table=11
                     /translation="MTSSDLGDFLRARRAHLRPGDVGLASYGRRRVAGLRREEVAVLAG
                     MNSDYYARLEQGRERGPSPQVLDAISAALRMDEAAREHLFRLAGTAPGGDRPQPREAVA
                     ASLRQLLDGYTSTPAFVLSPATDLLAANALADALFSPFAQRDNLARMTFLDPAARRFFV
                     HPDRAAEAVVAGLRHATGLDPHYPRLRDLVRELGSASADFAALWSAHTVRGKTYEAKEL
                     DHPDAGRLSLTFQSFDVRGAPGQQLVVYHAEPDSPSARALARLTPATSPPMR"
     gene            complement(2098..3657)
                     /locus_tag="B6R96_RS30360"
                     /old_locus_tag="B6R96_30360"
     CDS             complement(2098..3657)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151698.1"
                     /locus_tag="B6R96_RS30360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_30360"
                     /product="monooxygenase"
                     /protein_id="WP_081524190.1"
                     /transl_table=11
                     /translation="MDTDVIVVGSGPTGLLLACELALAGVRTLVVERRTEPQQDSRALT
                     LHPRSLELMDLRGLAPRFLGLGRTLPGWHFAALETPLDFAALDSRLAHTLFLAQARTEE
                     LLAQRAQELGVEIRRGHTLTGLRQGEDGVEAEVRGPQDGPGGRPGHGPAGTPRTLRAAY
                     LVGCDGGRSTVREAAGIDFPGTDETLTGVLGDFATVTAGPEALAAARGAGVLAVPLEGG
                     LTRFVYIDPERMRTPAQVPVTLEEFRSALVRLCGSDFGVDRPSWLSRFGNATRLAAAYR
                     SGRVLLAGDAAHIHFPAAGQGLNTGLQDAMNLGWKLAAEVRGWAPPGLLDTYHAERHPV
                     GRAVTDNTEVQTLLAELPLVDRYRRPAAALRGLFEELLRMPEVNRRFAEQVSALGTAYP
                     ATAPGADPLVGRRMPDIALSAAGSDATRVYELLHRGGFVLLRLAGARDPNPEPYEAPAR
                     HPHISAVTAHAYEEHPELDGVREVLVRPDGHIAWATRSADAGLRRTGRDAALTAWAVRS
                     PA"
     gene            3722..4453
                     /locus_tag="B6R96_RS30365"
                     /old_locus_tag="B6R96_30365"
     CDS             3722..4453
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565751.1"
                     /locus_tag="B6R96_RS30365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_30365"
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_107475606.1"
                     /transl_table=11
                     /translation="MSSPSTPARGRPARLDRTRTVDTALDLLDEVGLDALTMRRLADAM
                     DVQAGALYRYFATKGDLLTAMAERMLADLAATATTTATAKTEGAGDWSGELAALARAMR
                     TALLARRDGARVYAGTHATGPHTLGFAEAVIGVLCTAGFGAEEAARTLMAVVHLTIGHT
                     LEEQAVLRPVGSEGAADSERLRLAADPERYPRLAAVLPTLTSEDFAAHFEFGLSLLLRG
                     LGTLDRAPTCGDAPEIYSRGD"
     gene            4446..4811
                     /locus_tag="B6R96_RS30370"
                     /old_locus_tag="B6R96_30370"
     CDS             4446..4811
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137673.1"
                     /locus_tag="B6R96_RS30370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_30370"
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="WP_053704965.1"
                     /transl_table=11
                     /translation="MTENPLPPCPECAGAYTYEMGALLVCPECGHEWPHASGESDGSGG
                     AAEERVIKDAVGNVLADGDTVTVVKGLKVKGSPSGIKAGTKVRNIRLVEGVDGHDIDCR
                     IEGFGPMQLKSSVVRKV"
     gene            complement(5001..5972)
                     /locus_tag="B6R96_RS30375"
                     /old_locus_tag="B6R96_30375"
     CDS             complement(5001..5972)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="B6R96_RS30375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_30375"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_081524192.1"
                     /sec_met_domain="AfsA (E-value: 7.1e-22, bitscore: 76.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLSTDHSASPIATLSSALPREYVHKSAHSEVLLTGWRTVAPDEY
                     VVTAQWPRTHSFYTPDGGHHDPLLLAESVRQTIPLLSHVAYEVPFGHRQIWDTFSYSTN
                     PEALAVGSTPADIALHIRCSGISRRGRRLAGLTMHVTATRDGELLGTAEAGFTNQSEAV
                     YQRLRGGNADLDEVAARTIPLPPPLTPRRVGRDRFHDVVLSPTSSTRRAQLRADVNHPI
                     LFDHPVDHAPGMLLLEAVRQAAYRSAFPRRGVLTGMEIRFFRYAELTSACWIETVPIAG
                     DSTTPDRHPVRVVASQNGEVVFTATATINATAPAPHRIHATP"
     misc_feature    complement(5916..5918)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6043..6052
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     misc_feature    complement(6046..6059)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.43"
                     /tool="antismash"
     misc_feature    complement(6047..6060)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.51"
                     /tool="antismash"
     gene            6074..6787
                     /locus_tag="B6R96_RS30380"
                     /old_locus_tag="B6R96_30380"
     CDS             6074..6787
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883955.1"
                     /locus_tag="B6R96_RS30380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_30380"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_081524193.1"
                     /transl_table=11
                     /translation="MSERKQQRAPQLRAVQTKAAILRAAAEVFDEFGFSGASISKIMKR
                     ADVTQGGMYFHFSSKEELAYAVMVGQGEGLEFPAGEDGLQHLVDITLYLAEQLQHNPVL
                     RAGVRLAVEQGEFGLRDDVAYQAWVLEFRQQLRFARAKGELQPDVDDHELAWVLVSSFT
                     GAQLFSQASTGRADLPQRIASLWRYLLPAVAADDIRGGLRLTLPPTGPKPKNAPKNAPE
                     NAPTNETGSGAEKRA"
     gene            6784..7740
                     /locus_tag="B6R96_RS30385"
                     /old_locus_tag="B6R96_30385"
     CDS             6784..7740
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="B6R96_RS30385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_30385"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_081524194.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 4.5e-12, bitscore:
                     44.4, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTRRIVVTGATGFVGSAVLRRLAAQDPRAVVRATSRTPPPAATH
                     GVRWVDADLADEQSLRGVCEGADVLLSLASYIGPDAERCAAVNSAGAAALVAEARRAGV
                     ERIVHLSTCAVYGPGPHRGQDVAELTPAPVSPASAGRLAGEGPVLDAGGLVLRAALVLG
                     TGDRWAVPALVDAFRRVPARWGGGEGLASFVDVDDLARLIVAFALGGGAAARTTGVLHA
                     HHPEPVRNGDLMDALAEHRVLSARPEGDWPWHRCLEALAATPGWVSERQFALLARDHWY
                     RADAAWELAGITPGPGPLSRIGPAAAWYRERGGAAPG"
     gene            7846..9213
                     /locus_tag="B6R96_RS30390"
                     /old_locus_tag="B6R96_30390"
     CDS             7846..9213
                     /GO_function="GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382473.1"
                     /locus_tag="B6R96_RS30390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_30390"
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="WP_081524195.1"
                     /transl_table=11
                     /translation="MPDLSNIVKAYDVRGVVPDEWDESLAELFGAAFVEVTGADAIVIG
                     HDMRPSSPGLSGAFARGAAARGVDVTLIGLCSTDQLYYASGALGLPGAMFTASHNPAQY
                     NGIKMCRAGAAPVGQDTGLSQIRELVEKWSDGGAPAVASGTVAGTVTELDSLPGYAAHL
                     LGLVDLSSIRPLKVVVDAGNGMGGHTVPTVFEGLPLDVVPMYFELDGTFPNHEANPLDP
                     KNIVDLQARVLAEGADLGIAFDGDADRCFIVDERGVGVSPSAITALVAARELARNGGSG
                     TVIHNLITSWSVPEVVRENGGTPVRTRVGHSFIKEEMARTGAIFGGEHSAHYYFKDFWN
                     ADTGMLAALHVLAALGGQDGPLSELVASYDRYAGSGEINSTVADQGARLAAVKAAYGST
                     EGVTLDELDGLTVTAEDWWFNVRASNTEPLLRLNVEARDEATLAKVRDEALSLIRA"
     gene            complement(9386..10615)
                     /locus_tag="B6R96_RS30395"
                     /old_locus_tag="B6R96_30395"
     CDS             complement(9386..10615)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828668.1"
                     /locus_tag="B6R96_RS30395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_30395"
                     /product="transposase"
                     /protein_id="WP_081524196.1"
                     /transl_table=11
                     /translation="MTTRTRTRTLAAGDTELKEFTERLFGHLPRADQRRWARVYLQGLL
                     TTPGKKSVRRLAASVTESPTASQSLQQFINASPWDWNPARTELLRWVEERHPVRAWTIG
                     QVCFPKRGEHSVGVHRRFDPAAGRIVNCQLGFCLFLSLDGMNVPVDWRLALPEAWVTDP
                     VLRRRARIAPEAVHQSPEALVLELADSIAARTSSARPPVVADLSRLGGAAALIGMLGRR
                     GHDFLVSVPPTLAVRAAGLTPGADSTRTPGRGTTAADFSRLGNTSHPYGTPLAGPGARP
                     QGQRIHSALVRVPEGESGAGSPAGGQQIYRLFGERRPGAGGRGAGGGGGGGGPVWLTNM
                     NRHRMDDLLGLVRAAGRSAAVLGALADDFGLLDFEGRSFPGWHHHMTLMSAAYAFALRG
                     HVGLPRAQPA"
     misc_feature    complement(10532..10534)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10671..10684)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.56"
                     /tool="antismash"
     misc_feature    10679..10688
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
ORIGIN
        1 cggttcgagg tgttcgccat cgcggcgagg ctgcggcgtc cgtcgcgggt ggagaaggcg
       61 taggtgtaga aaccctgcac ggtgcccgtg tgcccgtaga ccgaggtgcc gcaggagagg
      121 tcgtagcggc gcaggccgag cccgtagaag cgggtgttcg tggcatcggt cggggtcgcg
      181 gtgagcatgg cgtccagcag gcgcgcgggc agcagccggc cgccgagcag cgcggaggtg
      241 aaggtgttca ggtcggcggg gttggagatc accgctcccg cggactgggc ccacgacacc
      301 gtctgctcgg tggagtcgac gagcggggcg cccgcctcgt cggggtggag gtagccgtgc
      361 gcgtgcagcc ccctgatctg cgttccgggg tgcacgtagg aggtgttgcg cagcttcagc
      421 ggcttgaaga tgcgccgctc gtactccttg gccacgccgt ggccggtggc cttctcgatg
      481 agcatgccga ggacgacgaa gttggtgttc gagtacgcgt acgcggcgcc gggctcgttc
      541 gtccgcggtt cggcgagcga gaggttcacc agctcctggt agctgaacac ccggttgcgc
      601 acggcctcga agcccggcac ggtgtgctcg aacatggcat tggtgtagtc cgacaggccg
      661 ctgcggtggc tcagcaggtg gcgcaccgtg atccggtcgt cgggcagcag tccgggcagg
      721 taccggttca cggcggtgtc cagatcgacc ttgccctcgt ccaccagctg cagcaggacg
      781 acggaggtga aggtcttgga gacgctcccg atccggaagc gggcctgcgg gtccatcgcc
      841 gaccccgtgg ccttgtcgcg caccccctcc gtccggacgc tgaccccgtc gggaccgctg
      901 aaacgggcca tggcgcccgg cgctccggcg gcggtcgtgt tccgcagcgc ctgcgacagc
      961 gcttcgaggt ccggggtggt cggggcggcg tgggcggtgg tcgccggagc cacggcggcc
     1021 atcaccccca gcagggctgc gccgagggcc aggcgtcggt tcatgggtcg gggcacgatg
     1081 ctcctccggt gggggcggaa tccggtccgc cggcgtcgaa cggccggtgt cgaccgttcg
     1141 ggtcgggcgg cggtgtcggt cgggtcggtc cggtcgggtc tgtcaggtcg gtcgctcacg
     1201 agcctacgca agatcatcag gtggggcggg cgccggaagc agcgggaacg cgccaataaa
     1261 caacggcagt tccgccacgc ggagccgcct tctaacctga aggtgtgacc agcagcgatc
     1321 tcggagactt cctgcgcgcc cggcgcgcgc acctgcggcc cggcgacgtg ggactcgcct
     1381 cctacggacg ccgccgcgtg gcagggctgc gccgcgagga ggtcgccgtc ctggccggaa
     1441 tgaacagcga ctactacgcc cggctggaac aggggcgcga gcgcggcccc tccccgcagg
     1501 tcctcgacgc gatcagcgcc gccctgcgca tggacgaggc cgcgcgggaa cacctgttca
     1561 ggctggcggg caccgcgccc ggcggtgacc ggccgcagcc acgggaggcg gtcgcggcct
     1621 ccctgcggca gctgctggac gggtacacga gcaccccggc gttcgtcctc agccccgcca
     1681 ccgacctctt ggcggccaat gccctggccg acgcgctctt ctccccgttc gcgcagcggg
     1741 acaacctcgc ccggatgacc ttcctcgacc ccgcggcccg acggttcttc gtgcaccccg
     1801 accgtgcggc cgaggcggtg gtggcgggtc tgcgccacgc gaccggcctc gacccgcact
     1861 acccgcggct gcgcgacctg gtccgtgaac tcggctcggc cagcgcggac ttcgccgccc
     1921 tgtggtccgc gcacaccgtc cggggcaaga cctacgaggc caaggaactc gaccaccccg
     1981 acgccggccg gctctcgctc accttccagt ccttcgacgt gcgcggggcg ccgggccagc
     2041 aactggtggt ctaccacgcc gaaccggaca gcccgagcgc ccgggccctc gcccggctca
     2101 cgccggcgac ctcaccgccc atgcggtgag cgccgcgtcg cgcccggtgc gccgcaggcc
     2161 ggcgtcggcg ctgcgggtgg cccaggcgat gtgcccgtcg gggcggacca ggacctcgcg
     2221 caccccgtcg agctccgggt gctcctcgta cgcgtgggcg gtgacggcac tgatgtgcgg
     2281 gtgccgggcg ggggcctcgt acggctccgg gttcgggtcc cgcgcaccgg cgaggcggag
     2341 caggacgaag ccgccccggt gcaggagctc gtacacccgt gtcgcgtccg aaccggccgc
     2401 gctcagggcg atgtcgggca tcctgcggcc gaccagggga tcggcgccgg gggcggtggc
     2461 cgggtaggcg gtgccgagcg cggagacctg ttcggcgaac cggcggttga cctcgggcat
     2521 ccgcagcagt tcctcgaaga gcccgcgcag ggcggcggcc ggccgccggt agcggtccac
     2581 gagcggcagc tccgccagca gcgtctgcac ctcggtgttg tcggtgacgg cccggccgac
     2641 ggggtggcgc tcggcgtggt aggtgtcgag gaggccgggc ggggcccagc ccctcacctc
     2701 cgcggccagt ttccagccga ggttcatggc gtcctggagc ccggtgttga ggccctggcc
     2761 cgcggcgggg aagtggatgt gggcggcgtc gccggccagt aggacacggc cggagcggta
     2821 ggcggccgcc agccgggtgg cattgccgaa gcgcgagagc cagctgggcc ggtccacccc
     2881 gaagtccgag ccgcagagcc ggaccagcgc gctgcggaac tcctccaggg tcaccggtac
     2941 ctgagccggt gtccgcatcc gctccgggtc gatgtacacg aagcgggtga gtccgccctc
     3001 cagcgggacg gcgagcacgc ccgcgccgcg ggcggcggcc agggcctcgg gaccggcggt
     3061 gacggtggcg aaatccccga gtacgccggt cagggtctcg tcggtgccgg ggaagtcgat
     3121 cccggccgcc tcccggacgg tgctgcgccc gccgtcgcat cccaccaggt acgcggcgcg
     3181 aagggtccgc ggggttccgg ccggcccgtg gcccggccgg ccgcccggcc cgtcctgagg
     3241 tccgcgcacc tctgcctcca ccccgtcctc gccctgccgc agcccggtga gcgtgtggcc
     3301 gcggcgtatc tccacgccca gctcctgcgc ccgctgtgcc agcagctcct cggtacgggc
     3361 ctgggcgagg aacagcgtgt gcgccagacg gctgtccagt gcggcgaagt ccagcggggt
     3421 ctccagggcg gcgaagtgcc agccgggcag cgtccggccg agcccgagga agcgcggtgc
     3481 gagaccgcgc aggtccatca gttcgaggct gcgcggatgc agggtcaggg cccgggagtc
     3541 ctgctggggc tcggtgcgcc gctcgacgac cagggtccgt acgccggcca gggccagctc
     3601 gcaggcgagc agcagcccgg tcggtcccga gcccacgacg atcacgtcgg tatccatcgg
     3661 tgatgcctcc cacaattagt gaacagcgtt cacgtgaacg ctgttcacta tagtgagggc
     3721 catgagctca ccgtcaaccc ccgcccgcgg ccgcccggcc cgcctcgacc gcacccggac
     3781 ggtcgacacg gccctggacc tcctcgacga ggtcggcctc gacgcactga cgatgcgccg
     3841 cctcgccgac gcgatggacg tccaggccgg cgcgctgtac cggtacttcg ccaccaaggg
     3901 cgacctgctg accgcgatgg cggaacggat gctcgccgac ctcgcggcga cagcgacgac
     3961 aacggcgacg gcgaagaccg agggcgccgg cgactggagc ggggaactgg ccgccctcgc
     4021 ccgggccatg cgtacggcgc tgctcgcccg ccgcgacggc gcacgcgtct acgcgggcac
     4081 ccatgcgacc ggcccccaca ccctcggctt cgccgaggcg gtcatcgggg tactgtgcac
     4141 ggccggattc ggggcggagg aggcggcgcg cacgctgatg gcggtggtcc acctgaccat
     4201 cggccacacg ctggaggagc aggcggtgct tcgaccggtg ggcagcgaag gggcggcgga
     4261 ttccgaacgc ctgcgcctgg cggccgatcc cgagcggtac ccgcggctcg ccgccgtgct
     4321 gcccacgctc acgagtgagg acttcgcggc gcacttcgaa ttcgggctgt ccctgctcct
     4381 gcgcgggctc gggaccctgg accgggcgcc gacctgcggc gatgcgcctg aaatatactc
     4441 gcggggtgac tgagaacccc ttgcctccct gccccgaatg tgcgggtgcg tacacctacg
     4501 agatgggggc gctcctggtc tgccccgagt gcgggcacga gtggccgcac gcctccggcg
     4561 agtccgacgg gtcgggcggc gccgccgagg agcgggtgat caaggatgcg gtgggcaatg
     4621 tgctggccga cggcgacacc gtgacggtcg tcaagggcct gaaggtcaag ggcagcccga
     4681 gcggcatcaa ggcgggcacc aaggtgcgca acatccgcct ggtggagggt gtcgacgggc
     4741 acgacatcga ctgcaggatc gagggcttcg gccccatgca gctgaagtcc agcgtggtca
     4801 ggaaggtctg accggcgcgg cgacgccggg aacggacgaa cggcgaaggc gcctgccggg
     4861 tacggacccg atcaggcgcc ttcgccgttc ttccgggggc cggagggccg gagggccgca
     4921 gagccggagg gaagggacgg gtcggttcgg cgggggctca tgagccggcg cgctcatgag
     4981 ccgggcgcct caccgaacgg tcagggcgtg gcgtggatgc ggtggggggc gggcgccgtg
     5041 gcgttgatcg tcgcggtggc ggtgaagacg acctcgccgt tctggctggc gaccacgcgt
     5101 acgggatggc ggtccggggt ggtcgaatcg ccggcgatcg ggacggtctc gatccagcag
     5161 gccgaggtca gctccgcgta gcggaagaag cggatctcca taccggtcag gactccccgt
     5221 cgggggaagg cggagcggta cgcggcctgg cgcacggctt ccaggagcag catgccgggg
     5281 gcgtggtcga cggggtggtc gaagaggatc gggtggttga cgtcggcccg gagctgggcg
     5341 cgccgcgtgg agctggtggg ggagaggacc acgtcgtgga agcggtcgcg gcccacgcgg
     5401 cggggtgtca gcggaggcgg cagcgggatg gtgcgggcgg ccacctcgtc gaggtccgcg
     5461 ttcccgccgc gcaggcgctg gtagacggcc tcggactggt tggtgaagcc ggcctcggcg
     5521 gtgccgagga gctctccgtc gcgcgtggcg gtcacgtgca tggtgagacc cgcgagtctt
     5581 cgtccgcgcc gcgagatgcc ggagcagcgg atgtgcagtg cgatgtcggc cggggtcgaa
     5641 ccgacggcga gggcctcggg gttggtcgag tagctgaagg tgtcccagat ctggcggtga
     5701 ccgaagggga cttcgtacgc gacgtggctc aggagcggga tggtctggcg gacggactcg
     5761 gcgaggagca ggggatcgtg gtggccgccg tccggcgtgt agaagctgtg ggtgcggggc
     5821 cattgggcgg tgacgacgta ctcgtccggc gcgacggtgc gccacccggt gagaaggact
     5881 tccgagtgtg cgctcttgtg tacgtactcc cgcggtaagg cgctgctgag cgtggcgatg
     5941 ggtgaggcgg agtgatcagt tgacagagtc atggttgagc ccccctggac gtcggacatg
     6001 agctggtgct tgttcggcgc gggtcctaaa ataaaggatg ttcgtttttt ttgtcgagat
     6061 tagttaggtg cccatgtcag aacggaagca gcaacgcgcc cctcaactca gggcagttca
     6121 gaccaaagct gccatcctgc gcgccgcggc cgaggtcttc gacgagttcg gtttcagcgg
     6181 ggccagcatc agcaagatca tgaagcgtgc ggacgtcacg cagggcggca tgtacttcca
     6241 cttcagctcg aaggaggaac tcgcctacgc cgtcatggtc ggccagggcg agggcctgga
     6301 gttccccgcc ggggaggacg gcctgcagca cctggtggac atcacgctct acctcgccga
     6361 acagctccag cacaatccgg tgctccgggc cggtgtacga ctggccgtgg aacagggcga
     6421 gttcggcctg cgcgacgacg tcgcctacca ggcctgggtc ctggaattcc gtcagcagct
     6481 gcgcttcgcc cgcgccaagg gggagctcca gccggacgtc gacgaccacg aactggcctg
     6541 ggtgctcgtc agctccttca ccggggcaca gctcttctcc caggcgtcca cgggacgggc
     6601 ggacctgccg cagcggatcg cctcgctgtg gcgctacctg ctgcccgcgg tggccgccga
     6661 cgacatccgc ggcgggctgc gcctgacact cccgcccacc gggccgaagc cgaagaacgc
     6721 gccgaagaac gcaccggaga acgcgccgac gaacgagacc gggtcgggcg cggagaagcg
     6781 ggcttgagca cccgccgcat cgtcgtcacg ggagcgaccg gtttcgtggg atcggcggtg
     6841 ctgcgccgcc tggccgcgca ggacccgcgg gccgtggtgc gcgccacgtc gcgtacgccg
     6901 ccccccgcgg caacgcacgg cgtgcgctgg gtggacgcgg acctggccga cgagcagtcc
     6961 ctgcgcgggg tgtgcgaggg cgcggacgtc ctgctgtcgc tggcctccta catcggaccg
     7021 gacgccgagc ggtgcgccgc ggtcaactcc gccggcgccg cggccctggt ggccgaggcc
     7081 cgaagggccg gagtggagcg gatcgtccac ctgtcgacct gcgccgtgta cggaccggga
     7141 ccgcaccggg gacaggacgt cgccgagctg acacccgccc cggtctcgcc ggccagcgcc
     7201 ggccggctgg cgggggaggg gcccgtgctc gacgccgggg gactggtcct gcgtgcggca
     7261 ctggtactcg gaacgggcga ccggtgggcc gtaccggccc tcgtcgacgc cttccggcgg
     7321 gtaccggccc gatggggcgg aggggagggg ctggcctcct tcgtcgacgt cgacgacctg
     7381 gcccgactca tcgtcgcctt cgccctcggc ggcggcgcgg cggcccggac gacgggtgtc
     7441 ctgcacgccc accacccgga gccagtacgc aacggggacc tcatggacgc gctcgccgag
     7501 caccgggtgc tgtcggcgcg gcccgaaggg gactggccct ggcacaggtg cctggaggcg
     7561 ctggccgcca ccccgggctg ggtgagcgaa cgtcagttcg cgctgctggc ccgggaccac
     7621 tggtaccgcg cggacgcggc ctgggaactc gccgggatca cgcccgggcc cggcccgctg
     7681 agccggatcg gtccggcggc ggcgtggtac cgggagaggg gcggggcggc cccggggtga
     7741 cggctatctg ccaaagcctc ggcgggatgg ggcgtgggcg ggctgtgccg ggcagggcga
     7801 gcggtagcgt ccggagcaca accggtcacc cctggaggag tggaaatgcc cgatctttcg
     7861 aacatcgtca aggcgtatga cgtgcgtggt gtcgtgccgg acgagtggga cgagtcgttg
     7921 gccgagttgt tcggtgcggc gttcgtcgag gtcacgggtg ctgatgcgat cgtgatcggt
     7981 catgacatgc ggccgtcctc cccgggtttg tcgggggcgt tcgcgcgggg tgcggcggcg
     8041 cgtggtgtgg atgtgacgtt gatcggtctg tgttcgacgg atcagttgta ttacgcgtcg
     8101 ggtgcgctgg gtctgccggg tgcgatgttc acggcgtcgc acaatcctgc gcagtacaac
     8161 gggatcaaga tgtgtcgtgc gggtgcggct ccggtgggtc aggacacggg tctgtcgcag
     8221 atccgtgagc tggtggagaa gtggtcggac gggggcgcgc cggcggtggc gtcgggcacg
     8281 gtggcgggga cggtcacgga gctggacagt ctgccggggt atgcggcgca tctgctgggt
     8341 ctggtggacc tgtcctcgat ccgtccgctg aaggtggtgg tggatgcggg caacgggatg
     8401 ggcggtcaca cggttccgac ggtgttcgag ggtctgccgt tggatgtggt gccgatgtac
     8461 ttcgagttgg acggcacgtt cccgaaccat gaggcgaatc ctctggatcc gaagaacatc
     8521 gtggacctcc aggcccgtgt ccttgccgag ggcgccgacc tcggtatcgc gttcgacggt
     8581 gacgcggacc ggtgtttcat cgtggacgag cggggtgtgg gggtgtcgcc gtcggcgatc
     8641 acggcgctgg tcgcggcgcg tgagctggcc cgtaatggtg gttcgggcac ggtgatccac
     8701 aatttgatca cttcgtggtc ggtgccggag gtggtccgtg agaacggcgg gacgcctgtg
     8761 cggacgcggg tgggtcattc cttcatcaag gaggagatgg cgaggacggg tgcgatcttc
     8821 ggtggtgagc actcggcgca ctattacttc aaggacttct ggaacgcgga cacggggatg
     8881 ctggcggcgt tgcatgtgct ggcggcgctg ggtggtcagg acgggccgtt gtcggagctg
     8941 gtggcttcct acgaccgtta cgcggggtcg ggggagatca actcgacggt cgccgatcag
     9001 ggggcgcgtc tggcggcggt gaaggccgcc tacggcagca ccgagggcgt gacgctggac
     9061 gagctggacg gtctgacggt gacggcggag gactggtggt tcaacgtccg cgcctcgaac
     9121 acggagccgc tgctgcggtt gaacgtggag gcccgtgacg aggccaccct ggccaaggtc
     9181 cgcgacgagg ccctgtccct catccgcgcc tgacccccgg tccgggccgc ccatctccag
     9241 ccccgccggc atgtgaggcg gggccggtac ggcaccgaag agcagggctc cgcccatccc
     9301 agcctcgccg gcgtttgagg cgcgggtccg ggcggagccc ggtgcccggc ggagccgggt
     9361 tccccccggg ccgcggcccg ccccctcagg cgggctgcgc ccggggaagc cccacatgcc
     9421 cccgcaaggc aaacgcatac gcggccgaca tcagcgtcat gtgatggtgc cagccgggaa
     9481 aggaccgccc ctcgaagtcg aggagcccga agtcgtccgc cagcgccccg agcacggcgg
     9541 cggaccgccc cgccgcccgc accaggccga gcaggtcgtc catccggtgc cggttcatat
     9601 tggtcagcca caccggccca cctccacccc cacccccacc ggcgccccgc ccgcccgccc
     9661 ccggccgccg ttccccgaac agccggtaga tctgctgccc gcccgcgggg ctccccgccc
     9721 ccgactcccc ctccggcacc ctgaccagcg ccgagtggat gcgctgcccc tgcggccgtg
     9781 cgccgggtcc cgcgagcggt gtgccgtacg ggtggctggt gttgcccagg cgggagaagt
     9841 cggccgcggt ggtcccgcgc ccgggcgtcc gggtgctgtc ggcgccgggc gtcaggccgg
     9901 ccgcgcgcac cgcgagggtc ggcggtacgg acaccaggaa gtcgtgcccg cggcggccga
     9961 gcatgccgat cagcgcggcg gccccgccga ggcggctgag gtcggcgacc accgggggac
    10021 gcgcggagga ggtgcgggcc gcgatcgagt cggcgagctc cagcaccagg gcttccgggc
    10081 tctggtggac ggcctcgggc gcgatgcgcg cccggcggcg caggacggga tccgttaccc
    10141 acgcctcggg gagggccagc cgccagtcga ccggcacgtt catgccgtcc agggagagga
    10201 agaggcagaa gcccaactgg cagttgacga tgcgtccggc ggcggggtcg aagcgtcggt
    10261 gcacgccgac cgagtgctcg ccgcgcttgg ggaagcagac ctgcccgatc gtccatgccc
    10321 gtaccgggtg tcgttcctcg acccagcgca gcagctcggt gcgggccgga ttccagtccc
    10381 aggggctggc gttgatgaac tgctgcaagg actgggaggc ggtgggcgac tcggtgacgg
    10441 acgcggcgag cctgcgtacg gacttcttcc ccggggtggt gagcagtcct tgcaggtaga
    10501 cccgggccca gcgtcgctgg tcggctctcg gtaagtgccc gaagaggcgc tccgtgaact
    10561 ccttcagttc ggtgtcaccg gcagccaggg ttctggtcct tgtccgtgtc gtcatctgtc
    10621 tccccctcca gtgcggatgt cggcaggcgc cgtcggtact cgtagaatac agaacgttcg
    10681 ttttttttct agcgaacgta tggcgtcctc cccgcgactt cggcacgttg gttcggggag
    10741 ggcggcggaa ccggtcacgg ggccggcacg gagctgcggg ggaacgcggg gcacggtcgc
    10801 ttcttcgtca agtcggcgtt gtggggggcc cgcccgcggg tgacgctgga ggtcgccatc
    10861 tccccgcggg gcccatggcg agtgaggaac gacctggatg gcagtgatag tgcagtggtg
    10921 aagcaagagc gcggcgtacg cacccgtaat catctgatct cggtggccgc cg
//
