LOCUS       NZ_CP020555            10960 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. Sge12 chromosome, complete genome.
ACCESSION   NZ_CP020555
VERSION     NZ_CP020555.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Sge12
  ORGANISM  Streptomyces sp. Sge12
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7983613)
  AUTHORS   Tao,M., Xu,J., Xu,M., Liu,K., Zhao,Z. and Peng,Q.
  TITLE     Genome sequence of Streptomyces sp. Sge12, producing unknown
            compounds against rice blast disease
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7983613)
  AUTHORS   Tao,M., Xu,J., Xu,M., Liu,K., Zhao,Z. and Peng,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, Huashan Road 1954, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: RS_HGAP Assembly v. 3; BWA v. 0.7.10; Bowtie v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.0x
            Sequencing Technology :: Illumina HiSeq 4000; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,312
            CDSs (total)                      :: 7,216
            Genes (coding)                    :: 7,106
            CDSs (with protein)               :: 7,106
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 32 of 111
            Pseudo Genes (incomplete)         :: 91 of 111
            Pseudo Genes (internal stop)      :: 8 of 111
            Pseudo Genes (multiple problems)  :: 18 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:35:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 150760
            Orig. end                         :: 161720
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020555.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10960
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="3"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10960
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10960
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5960](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5960
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(898..913)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.92"
                     /tool="antismash"
     misc_feature    930..944
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.18"
                     /tool="antismash"
     gene            complement(1112..2179)
                     /locus_tag="B6R96_RS38725"
     CDS             complement(1112..2179)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="B6R96_RS38725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_162498483.1"
                     /transl_table=11
                     /translation="MVKQERAARTREALVRAAAEVFAEEGFTAASIAVISRRARVSAGG
                     LHFHFAGKTVLARAVQDRAAQALSRITAGPAVPPTAPASAPTTPGPGPTTARTTTARTT
                     TARTTTARTTTARTTPAPGPASAPGPAPASAPTAPASAPGPAPGPAQGPAPASAPGSAP
                     ASAPGPASAPSAPAPGPASAATTPAPVPGPDGSAGTGADPGGNAGGSAGAGSGGGSLQV
                     LVDSTHRLMALLAHDAVVRAGFALCADPSFDGTADLRRQWRLWVEAAVAAAAREGLLAE
                     GLLPEDAARGVVAATVGLEVLGAYDRQWLAPHTLTRFWTLMLPRLASPPALDALLPQGS
                     PGSAAPPPDPDTESD"
     gene            complement(2353..3033)
                     /locus_tag="B6R96_RS00745"
                     /old_locus_tag="B6R96_00750"
     CDS             complement(2353..3033)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676872.1"
                     /locus_tag="B6R96_RS00745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_00750"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_030390789.1"
                     /transl_table=11
                     /translation="MARQERAVRTRRAILEAAAAVFDERGYDAATIADILARAGVTKGA
                     LYFHFSSKQELAQGVLDEQFAEGGVPHRESKLQELVDVAMVLAYRMKREPMLSAGARLS
                     LGPGMRDIFGGGSVPGWIEFTRTLLVRAKEQGELLPHVDPAETAWILSACWTGAQIYSQ
                     TLSDRSDIEQRVAAVYQHICPSIATPAMLARLDIAGDRGERVSAEMRRGPGRGAAQESE
                     PALS"
     gene            complement(3154..4512)
                     /locus_tag="B6R96_RS00750"
                     /old_locus_tag="B6R96_00755"
     CDS             complement(3154..4512)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385836.1"
                     /locus_tag="B6R96_RS00750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_00755"
                     /product="cytochrome P450"
                     /protein_id="WP_081521178.1"
                     /sec_met_domain="p450 (E-value: 1.2e-13, bitscore: 49.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRQCPFAIDALGGDVHAEAARVREQGPATRVLLPGGVSAWAVGR
                     AADMRRLLVDPRVSKDAYRHWPAWISGEVGEQWPLAIWVSVQNMITAYGSEHARLRRPA
                     AAAFTARRAAGLRPRIEEVVGELLEDLAASAPGGAVDLRTRFAHELPTRIVFELFGIPE
                     HSWGPLHKIINAFFDTGITAEEAQRNAADLDVVMADLVAHRRRHPGDDMTSALIAARDT
                     ATDPRTATDPRTATDARTATDARTATATAADAGAGAGAEGAGLSEKELVDSLILLFTAG
                     YETSVNLICTALHELLRNPAQLALVRAGLASWEDVVEEALRIEPPAFYGLLRFAVEDID
                     MGEGVVIGQGDPILISFGSAGRDPEVHGADADRFDVLRATRGQHLSFGFGTHHCLGATL
                     ARLECAVAVRRFFETFPAAALAGAGELGRVESFISNGLRELPVVLGPRAPVAD"
     misc_feature    4648..4663
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.25"
                     /tool="antismash"
     misc_feature    4977..4992
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.07"
                     /tool="antismash"
     gene            5001..5960
                     /locus_tag="B6R96_RS00755"
                     /old_locus_tag="B6R96_00760"
     CDS             5001..5960
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106284.1"
                     /locus_tag="B6R96_RS00755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_00760"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_237291262.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-20, bitscore: 72.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMLTIGKHAPVLPLPGTEQPWMRCNDARQLTTTVPREYVHRSSL
                     AEVFLTGCERTGQDTFTLTGQWPRAHTFFTSADGTGHDPVQAGETVRQVGLYLCHAVYG
                     VPLGHNVLLWSLDFTTRPEHLRIGRGPSDLTMNALCTDFGWKGNRFSGYLSVDIHRNGE
                     LAARGTARFSCVAPATYRRLRGDRGRLALTRPLPRRTPVPAHTTGRLLPFDVVLAPTDH
                     PGRWQLNPDLDHPILFEHANDHHPGMVLVEAARQAASALHAPATFTPAAIATEFHHYAE
                     LDAPCWIDATLTAPGHVAITGHQENRTIFRSTVTATTT"
     misc_feature    complement(6581..6595)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.8"
                     /tool="antismash"
     gene            7914..8318
                     /locus_tag="B6R96_RS00760"
                     /old_locus_tag="B6R96_00765"
     CDS             7914..8318
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552569.1"
                     /locus_tag="B6R96_RS00760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_00765"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_081521179.1"
                     /transl_table=11
                     /translation="MRIGELARRTGVSPRSLRYYEDQGLLTSSRSDAGQRHYADAAVQR
                     VALIRQLFDAGLSSRVIATVLPCVDIPGDPGVAEETFTAMMRERDRIDADIAHLIETRD
                     ALDALIRANSRHRAQLSTAPTTGGAADLML"
     gene            8417..9340
                     /locus_tag="B6R96_RS00765"
                     /old_locus_tag="B6R96_00770"
     CDS             8417..9340
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (22-189). E-value: 1.7e-29.
                     Score: 94.9. Matches aSDomain:
                     nrpspksdomains_B6R96_RS00765_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124491.1"
                     /locus_tag="B6R96_RS00765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_00770"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_107475436.1"
                     /sec_met_domain="ADH_N (E-value: 2.2e-08, bitscore: 32.6,
                     seeds: 66, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGQAWGFDRHGGPEVQAFFDRPDPVPGRGEVLIRVDVAGVNPLDH
                     LLRSGPVDGLDGGRPFPRVLGMEAAGTVLARGQDVTGLEVGDAVFGFALTGGGTYAQTT
                     VLSAANTARIPAGLSATVAATLPVAGTTAVDVLDQLGLPAGATVLVNGVGGGVGLAVTR
                     LAIGRRLHVIGTGSTAKREHAESLGALFIDYTTQDVAAAARKLVPDGFDAIVDLVAGTS
                     LRTIAPLARDPRNIIAVSDTSVPDLGGRFVERRLDRKNLERSARLALDGLLTPAITAVH
                     PLSEAPAALAAVENGHTTGKTVIKVT"
     aSDomain        8483..8983
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B6R96_RS00765_PKS_ER.1"
                     /evalue="1.70E-29"
                     /label="B6R96_RS00765_PKS_ER.1"
                     /locus_tag="B6R96_RS00765"
                     /protein_end="189"
                     /protein_start="22"
                     /score="94.9"
                     /tool="antismash"
                     /translation="DPVPGRGEVLIRVDVAGVNPLDHLLRSGPVDGLDGGRPFPRVLGM
                     EAAGTVLARGQDVTGLEVGDAVFGFALTGGGTYAQTTVLSAANTARIPAGLSATVAATL
                     PVAGTTAVDVLDQLGLPAGATVLVNGVGGGVGLAVTRLAIGRRLHVIGTGSTAKREHAE
                     SLGA"
     CDS_motif       8849..8911
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B6R96_RS00765_0001"
                     /evalue="1.90E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="B6R96_RS00765"
                     /protein_end="165"
                     /protein_start="144"
                     /score="11.0"
                     /tool="antismash"
                     /translation="GATVLVNGVGGGVGLAVTRLA"
     misc_feature    9466..9479
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    10438..10454
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 19.95"
                     /tool="antismash"
ORIGIN
        1 gctccgcgtg ggcctcggcc gggctctcgg gtccggccat caggatcgag cgctgcaggc
       61 ggcgcagggc gatggagggt tccagtccca gttcgcgcac gagggtggcg cgcagtcgct
      121 ggtagacgtc cagtgcctcg ccgcggcggc cggagcggtg cagggcgagc atgaactgtc
      181 cgtggaggtt ctcgtgggtg cggtagcggg tgaccaggac ggtcagttcg gccaggagtt
      241 cgcggtggcg tcccagtcgc aggtcggcct cgatccgctg gtccagggcg cacagccggg
      301 tctcttccag gcgtctggtc tccatgtcga gctggattcc gccctgtacg tcggcgaagg
      361 cgggtccgga ccacagggcc agggcctcgc gcagcaggcg ggcggcgccg gggaagtcgc
      421 cggcgtccat ggcgcggtag ccggtgccgg cgaggcggtc gaactcgcgg acgtcgaggg
      481 ttccgccgcc gctgctgagg aggtatccgc cgggcagggt gaccaggatg tccttggcgg
      541 tccgcaccac ccgccccgca cccgcacccg cacccgtacc ggtgggggtg tcgtcgtcgg
      601 ccccggcgtc ggggccggtg gcggtggcgg tgccgcgttc gagggcgccg gcgatgaggg
      661 cgcgtaactg caggacgtag gtctgcagtg tggtgcgggc actgcgcggc ggttcgcccg
      721 cccacagttc ctctatcagt gccgatacgg gtaccacctg atcggcatgg agggccagga
      781 gggccaggac ctgccggggc ttgggggcgg tcggcgtgat ggagattccg ttctcccgca
      841 ccgccaacgc gcccagtaca tcgatgtcca cgccgtactc ccctgtcctt ggatgagtcg
      901 cacagccagt agaaaacagg accggcggtt tgtcaatatc aaaccggtcg tgctgttttg
      961 atggtcgatc atgtccgacg gaacgtcaga aacctttgct ggcctggaat ttcgggtggg
     1021 ttcgggtgcg gtggggcggc ttcaggacgc ctcgatcacc ccctcgacga gggtcacagg
     1081 agggtggcgg accccacctg aaagagaccg ttcagtctga ttcggtgtcc gggtcggggg
     1141 gtggggcggc actgccgggc gatccctgcg gcagcagggc gtccagggcc gggggcgagg
     1201 cgagccgcgg cagcatcagc gtccagaacc gggtcagggt gtgcggtgcg agccactgcc
     1261 ggtcgtaggc gcccaggacc tccagcccca ccgtcgcggc caccaccccg cgcgccgcgt
     1321 cctcgggcag gagcccctcg gcgagcaggc cctcccgggc cgcggccgcc acggccgcct
     1381 cgacccacag ccgccactgc cggcgcaggt ccgccgtccc gtcgaaggag gggtccgcgc
     1441 acagcgcgaa cccggcccgc accaccgcgt cgtgcgcaag gagggccatc agccggtgcg
     1501 tggagtccac cagcacctgc aacgaacccc cgcccgaacc ggcgcccgca ctcccgcctg
     1561 cgttcccgcc cgggtctgcg ccggtgcccg cgctcccgtc cgggcccggg acgggggccg
     1621 gggtggtcgc tgcggaggca gggcccggtg cgggggcgga cggggcggag gcggggcccg
     1681 gggcggaggc gggggcggaa cccggggcgg aggcgggggc ggggccctgg gcggggcccg
     1741 gggcggggcc cggggcggag gcgggggcgg tcggcgcgga tgcgggagcc ggacccggcg
     1801 cggaggccgg acccggcgcg ggggtggtcc gggcggtggt ggtccgggcg gtggtggtcc
     1861 gggcggtggt ggtccgggcg gtggtggtcc gggcggtagt cggacccggt ccgggggtgg
     1921 tcggggcgga ggcgggggcg gtcggtggta cggccgggcc ggccgtgatg cgcgacaggg
     1981 cctgcgcggc ccggtcctgg acggcgcggg ccaggacggt cttgccggcg aagtggaagt
     2041 gcagtccccc ggcgctgacc cgggcccggc ggctgatcac cgcgatcgac gcggcggtga
     2101 acccctcctc ggcgaacacc tccgcggccg cacgcaccag cgcctcccgc gtacgggccg
     2161 cacgttcctg cttgaccatg gagtctccac aaaccgccaa cgcggtttgc ccaagggggt
     2221 gtaaagatcg ttccggtcac ggtccgcgcc gccaccggag ccccgctcgc gtcccgctgg
     2281 agccggccgg ccccaccggc cccacccgcc ccaccggggg ccgccctcgg gcgggcaggg
     2341 ggcgggcggg cctcaggaca gggccggttc gctctcctgc gccgcccccc ggccgggccc
     2401 gcgccgcatc tccgcgctca cccgctcccc ccggtcgccg gcgatgtcga gcctggcgag
     2461 catcgcgggc gtcgcgatgc tcgggcagat gtgctggtag acggcggcca cccgctgctc
     2521 gatgtcgctg cggtcgctca gggtctgcga gtagatctgc gcgcccgtcc agcaggcgga
     2581 caggatccag gccgtctcgg cgggatccac gtgcggcagc agctcaccct gctccttcgc
     2641 ccgcaccagc agggtccgcg tgaactcgat ccagcccggc accgaacccc cgccgaagat
     2701 gtcgcgcatg cccggcccca gagagagccg ggcacccgcg ctgagcatgg gctcgcgctt
     2761 catccggtac gccagcacca tcgcgacgtc caccagttcc tgcagcttgc tctcccggtg
     2821 gggcaccccg ccctcggcga actgctcgtc caggacgccc tgcgccagct cctgcttgga
     2881 ggagaaatgg aaatacaggg cgcccttggt gacgcccgcc cgcgcgagga tgtccgcgat
     2941 ggtggccgcg tcgtaaccgc gctcgtcgaa gaccgccgcc gccgcttcca agatcgcgcg
     3001 acgcgtacgt accgcacgct cctgccgtgc caccggtgcc tccgttgtcc atggtccaat
     3061 acggccggat ccaccgcgcg gatccaccgc cccattaaaa ccaaccagaa ggtccgattc
     3121 ttttcaagca ggccaggcgt cgccccgacg cggtcaatcg gccacgggcg ccctgggacc
     3181 cagcaccacg ggcaactccc gcagcccgtt ggagatgaac gactccaccc gccccaactc
     3241 ccccgccccc gccagagccg ccgccgggaa cgtctcgaag aaccgccgca ccgccaccgc
     3301 gcactccagc cgcgccagcg tcgcacccag gcagtgatgc gtgccgaacc cgaacgacag
     3361 atgctgcccc cgcgtcgccc gcagcacgtc gaagcggtcg gcgtccgcgc cgtgcacctc
     3421 gggatcacgc cccgccgaac cgaaggagat caggatcgga tcaccctgcc cgatcaccac
     3481 cccctccccc atgtcgatgt cctccaccgc gaaacgcagc agcccgtaga aggccggcgg
     3541 ctcgatccgc agcgcctcct ccaccacgtc ctcccacgaa gccaaccccg cccgcaccag
     3601 cgccaactgc gccggattgc gcagcagttc gtgcagggcc gtgcagatca ggttgacgga
     3661 ggtctcgtaa ccggccgtga agagcaggat caggctgtcg acgagctcct tctcgctcaa
     3721 ccccgccccc tccgcacccg cacccgcacc cgcatcggcg gcggtagcgg tagcggtacg
     3781 ggcgtcggtg gcggtacggg cgtcggtggc ggtgcggggg tcggtggcgg tgcgggggtc
     3841 ggtggcggtg tcgcgggcgg cgatgagtgc gctggtcatg tcgtcgccgg ggtggcggcg
     3901 gcggtgggcg accaggtccg ccatcaccac gtccagatcg gccgcgttgc gctgcgcctc
     3961 ctcggcggtg ataccggtgt cgaagaacgc gttgatgatt ttgtgcagcg ggccccagga
     4021 gtgctccggt atcccgaaca gctcgaagac gatccgggtc ggcagctcgt gggcgaaccg
     4081 cgtgcgcagg tccaccgcgc caccgggcgc cgaggcggcc aggtcctcca ggagttcacc
     4141 gaccacctcc tcgatccggg ggcgcaggcc ggccgcccgt cgggcggtga acgcggccgc
     4201 cgccggtctg cgcagccggg cgtgctcgct gccgtaggcg gtgatcatgt tctgcaccga
     4261 gacccagatc gccagcggcc actgctcccc cacctcgccg ctgatccagg ccggccagtg
     4321 gcggtaggcg tccttggaga cccgcgggtc caccaggagc cggcgcatgt cggccgcgcg
     4381 gcccacggcc caggcactga ccccgcccgg cagcaggacc cgtgtcgcgg gcccctgttc
     4441 acgcacgcgc gcggcctcgg cgtgcacgtc cccgcccagg gcgtcgatcg caaaagggca
     4501 ctgacggttc atctcacaac tctccaggat ggacggcggc agatcggcga cagggtgcag
     4561 cggcgtgtcc acaccccgta ttccaaaacc gcccatccgg tttctgtttc accggcggcg
     4621 gccccgagtc tgacacgacc cgacccgcat gccaatggtt ttcgggaccc ggcccgccgc
     4681 atccggtccc ccgaagtcgc cctggcgggg gcctggttga acggacggcg cccgcaccct
     4741 gtccgaccgg cgcatatgac cggaaccacg gttcccactt ggggcggtgg tgtgcgcgtc
     4801 ccctgcggcc gcaccctgtc ggatatccgc tggtccaggt cctgacagcc ctcccggagg
     4861 gcgcccggcg ggccgccggg gcgacgtgcg ggccgcatcc tgcccggctg agctccttgc
     4921 aaacaaaacc ggagagtacg tatcttgcgt ctcgcgagcg caacccgtcc catccgtcga
     4981 acggctctgc gggagaagtc atgaccatgc tgacgatcgg aaagcacgcg cccgtcctgc
     5041 ccctgcccgg gacggagcag ccatggatgc gctgcaacga cgcccgccag ctgaccacca
     5101 ccgtgccccg ggaatacgtc caccgcagct cactggccga ggtcttcctg accggctgcg
     5161 aacgcaccgg ccaggacacc ttcaccctga ccgggcagtg gccccgcgcc cacaccttct
     5221 tcaccagcgc cgacggcacc ggccacgacc ccgtccaggc cggcgagacc gtccgccagg
     5281 tcggcctcta cctctgccac gccgtctacg gagtccccct cggccacaac gtcctgctgt
     5341 ggagcctgga cttcaccacc cgccccgaac acctgcgcat cggccgcggc cccagcgacc
     5401 tcaccatgaa cgccctgtgc accgacttcg gctggaaggg caaccggttc tccggctacc
     5461 tcagcgtcga catccaccgc aacggcgaac tcgccgcccg cggcaccgcc cgcttctcct
     5521 gcgtcgcccc cgccacctac cgccgcctgc gcggcgaccg cggccgcctg gccctgaccc
     5581 gccccctgcc ccgccgcacc cccgtccccg cccacaccac cggccggctc ctgcccttcg
     5641 acgtcgtcct cgcccccacc gaccaccccg gccgctggca actcaacccc gacctcgacc
     5701 accccatcct cttcgaacac gccaacgacc accaccccgg catggtcctc gtcgaagccg
     5761 cccgccaggc cgccagcgcc ctccacgccc ccgccacctt cacgcccgcc gccatcgcca
     5821 ccgaattcca ccactacgcc gaactcgacg ccccctgctg gatcgacgcc accctcaccg
     5881 cccccggcca cgtcgccatc accggccacc aggaaaaccg caccatcttc cgctccaccg
     5941 tcaccgcaac caccacctga ccaccgctgt ccggcgtccg acgtcggggt cgggccgcgg
     6001 gcgtcggggt cgggggtgcc gcgggaacgg cgggggcagc gcggacatcg ggagccgggg
     6061 cggggcgagc cgacaggcac acaccccgcc cgcacaagag cgggcacacc ttccccggag
     6121 ggcggcttca ccaccgcgac cgggcaacac ccccagcacg caacaccgtt cacaccgggg
     6181 gccgggggcg gacaggcagg cgcacacccg cccgcacaaa aggggaaaca cctcccccgg
     6241 caacggcctc gccacccccg accggacaac accccccacc acgcgacacc gttcacaccg
     6301 ggggtcccgc cccatgcccc cgcacaccac cggcccagtc ggcgccgcag gaacccccgg
     6361 ccggaacacc ggcggggacc actcccagcg gcaccgaacg ccccccacca cggggcgcag
     6421 cacgcccaac aaagggggga gccgacccgg gcaaccgacc ggacacctcc cccgcagcag
     6481 cccgcgtcca caccgggccc ccgtccctga accagggggc gcgaacaccg ggggcaccgg
     6541 gatggactcg catcggctgc ccggggacag caccctttac cgacaacgac gttcacgcca
     6601 gccggagcca tgacggcgcc aagagcgcgc ccagcgcagc cggggctccg gtccggggcg
     6661 tcgtgccggc cccctcgcat cagccgctgt ccacgccggg cccccgcagc agcccgtggt
     6721 tcacgccagg accatcgccc cgcaccacgg cgcgggcagg cacgccgcac cagagtgtcc
     6781 ggccgggacc tcggagcggc cccgcacccc agccgccgcc cgggaaaccc ggcacgcagc
     6841 ggccgaacac ggtgcggccg cccggacggg atcggtcgga ccccctgcag caaccgcagt
     6901 ccacaccaac gccccggcac ggcacggcac ggcacgtggg ggcgcagtcc ccgaagcgga
     6961 ggaggctcat ccgcggacgg ccgccccgac aacctccccc accaccgccc atgttcatgc
     7021 cggagccgcc gccctgcgca cctgcaccac accgaaacgg gggtgtccgg tgccgggcga
     7081 gcagcccgac agccccgcac ggcagccccg ttcacgccag ggcccgtaac gagggcccgg
     7141 aggggcgcag gcaacgaaac gcaacggcac gcaacggaac gcaggggcga cccggcccgg
     7201 acaccgaccg caccacaccc gcccaagcgc acgatgcgca cgcccaggcc ctgccggtaa
     7261 accaggggcg tgcgggcacg ggacgggatc ccgccccggg catcgggaca gacacgcccc
     7321 tgcggcagcc gatgtccacg ccggggccgc aacccggcac gaaagttcag gcacgaaacc
     7381 aggggccgcc cgacccgggc gaccgacccg acagcacccg cgcccatccg gtgttcacgc
     7441 acggccacga cccaggaccc gggacccggg ccaccggtcc ccggacagca gggtcacccg
     7501 gcccaggacc tccgaccggc caccccctga cggcagcccg catccatacc aggccccccg
     7561 ccccggaacc aggggacgca gacaccggac acaaggacga cccgacccgg ctgcccccgg
     7621 cctggcaaca cccccggcgg cagcagaagt tcacgcccac cgagtgggat gtggcggcgt
     7681 catgggccgc ggggcgccga agccgggcgt gcccccgtcc ccgggcgccg caccgagccc
     7741 cctggcggca gcccgtgccg gcatacggcg acaagggctc agccgtgtac gggcgaacgt
     7801 gctcagccgt ggggcggcgg gcacggcaac cctgccgcgg tagggggtgt ccgcaggcga
     7861 ccatgacgca tcggcgctcc gcacccgcag gtcgcgggcg taggctctgc gggatgcgga
     7921 taggcgagct tgcccggcgc acgggcgtga gcccgcgctc actgcggtac tacgaggacc
     7981 aggggctgct gaccagctca cgctccgacg cggggcagcg tcactacgcc gatgctgcgg
     8041 tccagcgcgt ggcgctcatc aggcagttgt tcgacgcggg gctgtccagc cgggtgatcg
     8101 cgaccgtgct gccgtgtgtg gacatccccg gtgacccggg cgtcgccgag gagacgttca
     8161 cggcgatgat gcgcgagcgc gaccggatcg acgccgatat cgcgcacctg atcgagaccc
     8221 gggatgcact cgacgcactg atcagggcca acagccggca ccgggcgcag ctgtccacgg
     8281 ccccgacaac cggcggcgca gccgacctga tgctgtgacc tgcatctcat cgtgttgccc
     8341 ctgacatcag tggcaggggt gagggtggcg acagcgcccg cattcagcgg gccggtcgta
     8401 cgagaggcgg cggaagatgg gacaggcatg gggtttcgac aggcacggcg ggcccgaggt
     8461 gcaggcgttc ttcgatcgcc ccgatcccgt acccggtcgc ggtgaggtac tgatccgggt
     8521 cgacgtcgcc ggcgtcaatc ccctcgacca cctgctgcgc tcgggcccgg tcgacgggct
     8581 cgacggcggg cgtccctttc cccgcgtgct gggcatggag gcagccggaa ccgttctcgc
     8641 ccggggccag gacgtcaccg ggctcgaggt gggcgacgca gtcttcggct tcgcgctgac
     8701 cggtggcggc acctacgccc agaccacggt gctgtccgcg gcgaacaccg cgcgcatccc
     8761 ggcgggcctg tccgcaaccg tggcggcaac actgccggtg gccgggacga ccgcggtgga
     8821 cgtcctcgac caactcggac tccccgccgg cgccacggtc ctggtcaacg gagtcggagg
     8881 cggagtcggc ctcgccgtca cccggctggc catcggacgc cggctgcacg tgatcggcac
     8941 cgggagcacc gccaaacgcg aacacgccga gagcctgggg gcgctcttca tcgactacac
     9001 cacccaggac gtcgccgccg cggcacgcaa gctcgttccg gacggcttcg acgcgatcgt
     9061 cgacctggtc gcaggcacct cactgcgaac catcgctccc ctggcccgcg acccccgcaa
     9121 catcattgcc gtcagcgaca catccgtgcc cgatctcggc gggcgcttcg tcgagcgccg
     9181 cctcgaccgc aagaacctgg agcgatcagc ccgactcgcc ctcgacggac tcctcacacc
     9241 cgcaatcacc gcagtccatc cgctctccga agccccggcc gccctcgccg ccgtcgagaa
     9301 cggccacacc accggcaaga ccgtcatcaa ggtgacataa gaaacacccg gccacccgac
     9361 aacgtcaacc ggccaaggcg gaccgccaag aacgacaacc tcccgaggca gagccgggac
     9421 gaacgtgacc cacggtcggc gcaccatcga ccgagcacat tcacctgtac cgagtcgaac
     9481 cccttcaccg gtacgccacc agcccgcgcc cacaccaagc caccacccca ccacccgggg
     9541 cccaagacac cggacacaag gaagacccag cccggctctc cggcggacaa cacactgcgt
     9601 gacacacgat gttcacgccc tgccaaaccc gtggcggcgc ccagggcgca tgggccggcc
     9661 caggaggggg agagcatcgg ggccgtcagg cagggttccc gcccgacagc accgaccggt
     9721 accacggagc acactccgcg aacgcctcca cgaacccagg ccccggcgaa accctcacct
     9781 gcgaccacaa ccgcccactg tcataccagc gatcaacgcc caccaaccgc acccgccgcg
     9841 ccaacacccc accctgcgca ccccccaccc actccaacgc cccgccagat caacctcccc
     9901 caccggcaac cccaacccca acgcacccgc aaccgtctcc accaacaccc gcacactcac
     9961 cggctccgga tgccccgcat gcaacacccc acccccaccc gcccaccccc accgccaact
    10021 ccgccaacac ccccgccaac acatccaccg aaaccaacga caaacgcgcc cgcccaccct
    10081 ccacccacac cggcacacgc cccaacaact ccaccaaccc cggcaccacc caccgatccc
    10141 cccgcccgaa aaccaaatgc ggccgcacca ccgtcccacc cgccgccaac accaaccgct
    10201 cccccgccaa ccgcgtcaca ctcgtcaccg accccggaca ctccaccaac tccccctcca
    10261 ccacccccga tgcaccccat ccccatacac ccccgccgtc cccaactgca ccacccgctc
    10321 cacccccgcc cgcgccgcct ccccaacaac gcccgcgtcc cctccacatt caccgcctcc
    10381 aactccccca ccgacccccc gatccgcgac gccaaatgca acaccgacga caccccctca
    10441 cacaccccac gcaacgaacc cggccgcacc aaatcccccg caccacctcc acccccaccc
    10501 gcgacacccc cgcccgatgc accaacaccc tcaccccaac accccacccc accaacctct
    10561 ccaccaccac agaacccaca aaccccgcac cccccgtcac caacaccaac ccacccccac
    10621 cgaacaccac aaccccccaa tccaccgtgt taccgtcacg cgatgaccgc cggaacccaa
    10681 cccaagctcc tcgaaggcag atccgcactc atcaccggcg caagctccgg catcggagcc
    10741 gccgccgccc gcatcttctg ccgcgaaggc gccgccgtca ccctcgtcgc ccgccgcgaa
    10801 aaacaactcg ccgacctcac cgacgaacta caaacccaag gccaccacgc ccaatacgtc
    10861 atcgccgacg tcacccacac cgaacaaatc gcccacgccg tccaacaagc catcaccacc
    10921 tacggccacc tcgacaccgc cttcaacaac gccggcatcg
//
