LOCUS       NZ_CP017717            10816 bp    DNA     circular CON 03-MAR-2024
DEFINITION  [Actinomadura] parvosata subsp. kistnae strain ATCC 55076
            chromosome, complete genome.
ACCESSION   NZ_CP017717
VERSION     NZ_CP017717.1
KEYWORDS    RefSeq.
SOURCE      [Actinomadura] parvosata subsp. kistnae
  ORGANISM  [Actinomadura] parvosata subsp. kistnae
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1  (bases 1 to 13047416)
  AUTHORS   Nazari,B., Forneris,C.C., Gibson,M.I., Moon,K., Schramma,K.R. and
            Setedsayamdost,M.R.
  TITLE     Nonomuraea sp. ATCC 55076 harbors the largest actinomycete
            chromosome to date and the kistamicin biosynthetic gene cluster
  JOURNAL   Medchemcomm (2017) In press
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13047416)
  AUTHORS   Nazari,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2016) Chemistry, Princeton University, 20
            Washington Road, Princeton, NJ 08544, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 18-JUN-2015
            Assembly Method                   :: Celera Assembler v. Jun 2015
            Assembly Name                     :: SmrtPipe 1.87.139483
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 13:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12,000
            CDSs (total)                      :: 11,919
            Genes (coding)                    :: 11,783
            CDSs (with protein)               :: 11,783
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 36 of 136
            Pseudo Genes (incomplete)         :: 98 of 136
            Pseudo Genes (internal stop)      :: 10 of 136
            Pseudo Genes (multiple problems)  :: 6 of 136
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:57:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 11337350
            Orig. end                         :: 11348166
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017717.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="32"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5816
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            23..1210
                     /locus_tag="BKM31_RS51600"
                     /old_locus_tag="BKM31_51220"
     CDS             23..1210
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /locus_tag="BKM31_RS51600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_51220"
                     /product="C40 family peptidase"
                     /protein_id="WP_230465786.1"
                     /transl_table=11
                     /translation="MPFIVVVLVAVLTADVLLLTRPDASLAPGEAVRATPRQTPLAQKR
                     PLRQQPPAQQTQPPAQQPVTTGQPVAPLSKLHKPNLFVLTSTPFTNEMLQKVARLSGVR
                     ALELADAASVTLDGKRVQTLAVNPSTFRSYTPKVTASSDALWQNVAAGDVAVSFVLGND
                     GGLTLHSRVSAGGSRQLRIGAYATTGFGAVDAVVSKEVGRALGLPRDNALIVSAPKANS
                     DTLARKIKKVLGKGTQVATVNPVLPQPRQKVTERSWPAGSFMTGEQLTVALRAAVSKLG
                     RPYVWGAEGPDTFDCSGLVQWAYGQAGVRMPRVTHQQWVTGPQVPLSQAQPGDLLFWRN
                     DPTNPDYISHVAIYWGNGKMLHAPRTGDVVKIAPVNTRNLAGVVRVSPVAAAKVR"
     misc_feature    74..76
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1267..1280
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.19"
                     /tool="antismash"
     misc_feature    complement(1269..1282)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.19"
                     /tool="antismash"
     gene            1282..1803
                     /locus_tag="BKM31_RS51605"
                     /old_locus_tag="BKM31_51225"
     CDS             1282..1803
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894902.1"
                     /locus_tag="BKM31_RS51605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_51225"
                     /product="hypothetical protein"
                     /protein_id="WP_080045134.1"
                     /transl_table=11
                     /translation="MATDDTTAQVRAWFTGRLPEGWFTAAPEIVRDREEIAVLGTLPDP
                     PADTPEAERPALIEGLARRFREETRERRIEIAREAERRFRQKVSWGVVCGGETVMFTTL
                     SVPVMTRLRQAERQVLDTLVAAGVARSRSDALAWCVRLVGKNTDEWLAELRDALQHVDR
                     VRSAGPDVHS"
     misc_feature    1829..1844
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.51"
                     /tool="antismash"
     misc_feature    1851..1866
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.39"
                     /tool="antismash"
     misc_feature    1870..1885
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.86"
                     /tool="antismash"
     misc_feature    complement(1985..2000)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.63"
                     /tool="antismash"
     gene            2064..3857
                     /locus_tag="BKM31_RS51610"
                     /old_locus_tag="BKM31_51230"
     CDS             2064..3857
                     /EC_number="4.1.1.32"
                     /GO_function="GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0017076 - purine nucleotide binding
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133322.1"
                     /locus_tag="BKM31_RS51610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_51230"
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="WP_080045135.1"
                     /transl_table=11
                     /translation="MSVSVEVEAPTSNSQLAAWVNEIAALTQPDRVEWCDGSEEEWTRL
                     TNLLVEQGTLTRLAARPNSFYAKSDPTDVARVEDRTFICSERREDAGPTNNWIAPAEMR
                     QTFGELFKGCMRGRTMYVVPFCMGPLGGEISQLGVEITDSPYVAVSMRIMTRMGDRALR
                     LIEERGDFVKAVHSVGAPLEPGQADVPWPCSSTKYISHFPETREIWSYGSGYGGNALLG
                     KKCYALRIASVMARDEGWLAEHMLILKLTPPSGETRYIAAAFPSACGKTNLAMLKPTIP
                     GWKVETIGDDIAWMRFGEDGRLYAINPEAGFFGVAPGTGQVTNANAIETLWGNSIFTNV
                     ALTDDGDVWWEGLTEEPPAHLTDWKGRSWTPDSDEPAAHPNARFTTPASQCPTIAPEWQ
                     DPKGVPISAILFGGRRATAVPLVTESLSWQHGVFLGANVASEKTAAAEGKVGELRHDPF
                     AMLPFCGYNMGDYFGHWLEIGRREGAELPRIYYVNWFRKNDEGKFVWPGFGENSRVLKW
                     IVERLNGEAKAVPTPIGLLPADLDTQGLDLSEEDLRLLLTVDRDVWREEAALIPAHFDT
                     FGSHLPKELWDEYNALLDRLG"
     gene            4045..4998
                     /locus_tag="BKM31_RS51615"
                     /old_locus_tag="BKM31_51235"
     CDS             4045..4998
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="BKM31_RS51615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_51235"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_080045136.1"
                     /transl_table=11
                     /translation="MDLFLLIVSVAIAAYAAVTTSAITRAHAAIRSAATGSGLGELGHY
                     DLAYLVGGPTRVADTAIAILAERHELRVSRGGRVHRVITSTTAREPIEEAVLGVVATRS
                     GLPVMSLRIEAVRSLAMEEIKQRLTAQGLLLPDGAFVQARRLSVRLRVLSVPAYAGFAI
                     EGLTMALAPNLHALLATLVFLGTMIGAEAVLGNHRRIVRHTLTESGRDALDSARRAHAP
                     GADGPVPLALYGLGELRDAKLRAELSRRAPQRERRPRSSYAGSGSSRRGGAWYGGSGSH
                     GGGSYGCGGGSSSCGSGSSGCGGGGGCGGGGCGGGS"
     gene            5001..5816
                     /locus_tag="BKM31_RS51620"
                     /old_locus_tag="BKM31_51240"
     CDS             5001..5816
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="BKM31_RS51620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_51240"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_080045137.1"
                     /sec_met_domain="DUF692 (E-value: 2.3e-80, bitscore: 269.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASLGVGIGWRPELDLTIERMGGVDFLEVVAENIRPGRLPESLRV
                     LRARGLPVIPHGVSLGVGGAEPPSPARLAHLAACAQALDAPLVSEHLAFVRGGGIEAGH
                     LLPVPRTRESLKVVIENVRRAQDALPVPLALENVAAVFGWPDDELTEAQFLAELVAATG
                     VGLLIDVANLYTNQVNLGLPAVAALDTLPLEHLAYVHVAGGYEHHGIWHDTHTTTAPQA
                     VLDLLTELCSRVTPPGVLLEWDDEYPSDAVLADELSRIRAAMAVPGQAR"
     gene            5813..6334
                     /locus_tag="BKM31_RS63810"
     CDS             5813..6334
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BKM31_RS63810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_222107629.1"
                     /transl_table=11
                     /translation="MTPMEARRLAGAQGPPTQPPQLTAPPLEVSEQAAQEQAAQEQAAP
                     ERVSPEEARERDEERRRRLAEAQGRVVAALVAGAEVPEGFDLERMRAQAVSLVSKRRAI
                     VARLRPDAAAAAGPDLAAEFAAYARARATPPPGYRADADDFAAWLRERGRMPGEPPPKR
                     RWWSRLPGIR"
     gene            6373..7242
                     /locus_tag="BKM31_RS51630"
                     /old_locus_tag="BKM31_51250"
     CDS             6373..7242
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545139.1"
                     /locus_tag="BKM31_RS51630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_51250"
                     /product="hypothetical protein"
                     /protein_id="WP_080047481.1"
                     /transl_table=11
                     /translation="MRIVNMGEWPLGPEDERRHAPGGEPLWNESWYYDFVSEDGRAGGY
                     VRLGLYPAWGRAWYWACLVTADGDRVEVIDHEAALPRGDLAVAGPGYTAAHATTDPFER
                     AHIRLSSAELSLDLEFRTAGGAYGYALTPRYEVPCEVTGTIGGLPFRGHGERDHSWGVR
                     DWWSISWLWSSARLDDGTYLHGMRANVGMELPWPAFLAAKGDLAHVEGFGAATVFDGDR
                     PVESVLRFPGCVTTVRPVAFAPVTLTSPEGVAARFARALCAYETEDGRTGHGWTEWHQP
                     PGWREHVW"
     gene            7340..7852
                     /locus_tag="BKM31_RS51635"
                     /old_locus_tag="BKM31_51255"
     CDS             7340..7852
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653040.1"
                     /locus_tag="BKM31_RS51635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_51255"
                     /product="hypothetical protein"
                     /protein_id="WP_080045138.1"
                     /transl_table=11
                     /translation="MGGLRCVDGSYLSVTPVACTDSVGAPYEITLELHRDGTPYGSVGE
                     RCGHLLARLARGVDEARQGRGAARRWADPDDRFPGEGPDGELFSFRYLTRAGLGGGELR
                     CQLRTIPVWQPGPGEWRVTRRAFVEAWGATGVGMRAVLTSAELSVFVRELVDEAEGCLK
                     GRDSAEE"
     misc_feature    complement(7911..7930)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: medium, score: 24.02"
                     /tool="antismash"
     gene            7945..8706
                     /locus_tag="BKM31_RS51640"
                     /old_locus_tag="BKM31_51260"
     CDS             7945..8706
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545137.1"
                     /locus_tag="BKM31_RS51640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_51260"
                     /product="isoprenyl transferase"
                     /protein_id="WP_080045139.1"
                     /transl_table=11
                     /translation="MGLRDLVYRLYERRLEAKLSKDSIPRHVGVIIDGNRRWARAMGLR
                     DVSTGHRKGADKISELLGWCRELGVEVVTVWMLSNDNLNRPREELEPLLRIIEDVTREL
                     VAEGWRISPVGALDLLPDRTANVLKNAKEASSHRPGLIVNVAVGYGGRREIADAVRSLL
                     QEHASRGASIEDLVEVLDVEHIAEHLYTRGQPDPDLLIRTSGEQRLSGFMLWQSAHSEF
                     YFHEAYWPDFRRVDFLRALRDYAARHRRYGS"
     misc_feature    complement(8939..8954)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.41"
                     /tool="antismash"
     gene            8965..10233
                     /locus_tag="BKM31_RS51645"
                     /old_locus_tag="BKM31_51265"
     CDS             8965..10233
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545136.1"
                     /locus_tag="BKM31_RS51645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_51265"
                     /product="PhoH family protein"
                     /protein_id="WP_080047482.1"
                     /transl_table=11
                     /translation="MLDTSVLLADPAAMTRFAEHDVVLPIVVITELEGKRHHPELGYFA
                     RKALRYLDDLRVQYGRLDEAMPTGDGTIRVELNHSDPSILPAGFRLGDNDTRILTVARS
                     LASEGCDVVLVSKDLPMRVKAASIGLAAEEYRAELVHESGWTGMAEVQVTAEDVETLFE
                     TGTADMEEARDLPTHTGLRLLSAKGSALGRVQPDKSVRLVRGDREVFGLHGRSAEQRIA
                     LDLLMDPEIGIVSLGGRAGTGKSALALCAGLEAVLERRQHRKVIVFRPIYAVGGQELGY
                     LPGTEGEKMGPWGQAVYDTLGAITSPEVIEEVLDRGMLEVLPLTHIRGRSLHDAFVIVD
                     EAQSLERGVLLTVLSRIGANSRVVLTHDVAQRDNLRVGRHDGVVAVVEKLKGHPLFAHV
                     TLTRSERSPIAALVTEMLEDITM"
     gene            complement(10288..10785)
                     /locus_tag="BKM31_RS51650"
                     /old_locus_tag="BKM31_51270"
     CDS             complement(10288..10785)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BKM31_RS51650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BKM31_51270"
                     /product="hypothetical protein"
                     /protein_id="WP_155129073.1"
                     /transl_table=11
                     /translation="MRALVAVVLLLAMTGCGILPTDVQDRGSAPTVRIPPPTKTIFLLR
                     NGKLMREPADVEDDTVDSVLTALFDASAHPLGDRDTALRGFTYLRTRNSINPPQRDEIQ
                     LPRTSTLTVYISGDGTLTKLGMAQIVCTAQQDAAFEVVKIVRENEGRPPKDEGRHTCPE
                     LN"
ORIGIN
        1 gcggcgcaac acgtacaagc tggtgccgtt catcgtggtg gtcctggtcg cggtgctgac
       61 cgccgacgtg ctgttactga cccggccgga cgcctcgctg gcgcccggcg aggccgtgcg
      121 cgccaccccc aggcagacgc cgctcgcgca gaagcggccc ctgcggcagc agccgcccgc
      181 ccagcagacg cagccgccgg cccagcagcc cgtcacgacg gggcagccgg tggcgccgct
      241 gagcaagctg cacaagccga acctgttcgt gctcaccagc acgccgttca cgaacgagat
      301 gctgcagaag gtcgcgaggc tgtccggcgt gcgggcgctg gagctggccg acgcggcctc
      361 ggtcacgctc gacggcaagc gggtgcagac gctggccgtc aacccctcca ccttccgctc
      421 ctacacgccc aaggtgaccg cctcttctga cgcgctgtgg cagaacgtcg cggccgggga
      481 cgtggcggtc tcgttcgtgc tcggcaacga cggcggcctg acgctgcaca gccgggtgtc
      541 ggcgggcggg agccggcagc tgcgcatcgg cgcgtacgcc accacggggt tcggcgccgt
      601 ggacgcggtc gtgtccaagg aggtcggccg cgcgctgggg ctgccccgcg acaacgcgct
      661 catcgtcagc gcgccgaagg cgaactccga cacactggcc aggaagatca agaaggtgct
      721 gggcaagggc acgcaggtgg ccacggtcaa cccggtgctg ccgcagccgc ggcagaaggt
      781 gaccgagcgg agctggccgg cgggctcgtt catgaccggt gagcagctca cggtggcgct
      841 ccgggcggcg gtctccaagc tgggccgccc gtacgtgtgg ggcgccgagg gccccgacac
      901 cttcgactgc tccggcctgg tccagtgggc gtacgggcag gcgggggtgc ggatgccgcg
      961 ggtgacgcac cagcagtggg tgacggggcc gcaggtgccg ctgtcgcagg cgcagcccgg
     1021 tgacctgctc ttctggcgga acgacccgac gaaccccgac tacatctccc atgtggcgat
     1081 ttattggggt aacgggaaga tgctccacgc accccgtacc ggtgacgtcg tgaagatcgc
     1141 cccggtgaac accaggaacc tggcgggcgt cgtccgggtc agccccgtcg cggcggccaa
     1201 ggtccgctga cgcctttcct ccacggcagg tccttctagc cttgcgtgcg ttgcggctcg
     1261 caagagtgta atgatgatta catggcaaca gacgacacga ccgcgcaggt gcgggcctgg
     1321 ttcaccggcc gcctgcccga aggctggttc accgcggcgc ccgagatcgt cagggaccgc
     1381 gaggagatcg cggtgctcgg caccctgccc gacccgcccg ccgacacgcc cgaggccgag
     1441 cggccggccc tgatcgaggg gctggccagg cggttcaggg aggagacgcg ggagcggcgc
     1501 atcgagatcg ccagggaggc cgagcgccgg ttccgccaga aggtgtcgtg gggcgtggtc
     1561 tgcggcggcg agactgtgat gttcaccaca ctctccgtgc ccgtgatgac gcggctgcgc
     1621 caggccgagc gccaggtgct cgacacgctc gtggccgccg gcgtggccag gagccgcagc
     1681 gacgcgctcg cctggtgcgt ccgcctcgtc gggaagaaca ccgacgaatg gctcgccgag
     1741 ctgcgcgacg ccctgcagca cgtcgaccgc gtccgctccg cggggcccga cgtccattca
     1801 tagaccgtcc agcggaaacg gctcacggaa aggactaaac cactgcgcta attggtttag
     1861 accagcttaa ttggactgga ccactggcct ccgaaggcgc cggaaattgc tcttttaaat
     1921 ccttcgcggc tgggtaggct cccccaatca tcagggccgc tgcctgcggc gacacgcccg
     1981 gccgacaggt ctacgccaat tggctttccg ggtgcttcgt cgttaatgat gactgggccc
     2041 tgagagagtg gaggagtcaa gtcgtgtccg tttccgtgga agtagaagcg cccaccagca
     2101 atagccaatt ggccgcgtgg gtgaacgaga tcgcagccct cacccagccg gaccgcgtcg
     2161 agtggtgcga cgggtcggag gaggagtgga ctcggctcac caacctcctc gtggagcagg
     2221 gcacgctcac gcgtctggcc gcccggccca acagcttcta cgccaagtcc gaccccaccg
     2281 acgtcgcgcg ggtcgaggac cgtaccttca tctgctccga gcgccgcgag gacgccgggc
     2341 ccaccaacaa ctggatcgcg cccgccgaga tgcgccagac gttcggcgag ctgttcaagg
     2401 gctgcatgcg cggccgcacc atgtacgtgg tgccgttctg catgggcccg ctgggcggcg
     2461 aaatctccca gctcggggtg gagatcaccg actccccgta cgtcgccgtc tccatgcgga
     2521 tcatgacccg gatgggggat cgggcgctgc ggctcatcga ggaacgcggc gacttcgtca
     2581 aggcggtcca ctccgtcggg gccccgctgg agcccggcca ggcggacgtg ccgtggccgt
     2641 gcagctccac caagtacatc agccacttcc ccgagacccg cgagatctgg tcctacggat
     2701 ccggctacgg cggcaacgcg ctgctcggca agaagtgcta cgcgctgcgc atcgccagcg
     2761 tgatggcccg cgacgagggc tggctggccg agcacatgct catactcaaa ctcacgccgc
     2821 cctccggcga gacccgctac atcgccgcgg ccttccccag cgcctgcggc aagaccaacc
     2881 tcgccatgct caagcccacg atcccgggct ggaaggtcga gacgatcggc gacgacatcg
     2941 cctggatgcg gttcggcgag gacggccgcc tgtacgcgat caacccggag gccggcttct
     3001 tcggcgtggc gcccggcacc ggccaggtca ccaacgccaa cgccatcgag acgctgtggg
     3061 gcaacagcat cttcaccaac gtcgcgctca ccgacgacgg cgacgtgtgg tgggagggcc
     3121 tgaccgagga gccgccggcc cacctcaccg actggaaggg ccgctcctgg acgcccgaca
     3181 gcgacgagcc ggccgcccac cccaacgccc gcttcaccac gccggcctcg cagtgcccga
     3241 ccatcgcgcc cgaatggcag gaccccaagg gcgtgcccat ctcggcgatc ctgttcggcg
     3301 gccgccgcgc caccgccgtg cctctggtca cggagtcgct gagctggcag cacggcgtct
     3361 tcctcggagc caacgtcgcc tccgagaaga ccgccgccgc cgagggcaag gtcggcgagc
     3421 tgcgccacga cccgttcgcc atgctgccgt tctgcggcta caacatgggc gactacttcg
     3481 gccactggct ggagatcggc cgccgcgagg gcgccgagct gccgcgcatc tactacgtca
     3541 actggttcag gaagaacgac gagggcaagt tcgtctggcc cggcttcggc gagaacagcc
     3601 gcgtgctgaa gtggatcgtg gagcggctca acggcgaggc caaggccgtg cccacgccga
     3661 tcggcctgct gcccgccgac ctcgacacgc agggcctcga cctgtcggag gaggacctgc
     3721 gcctgctgct gaccgtcgac cgggacgtgt ggcgcgagga ggcggcgctg atccccgccc
     3781 acttcgacac gttcggcagc cacctgccca aggagctgtg ggacgagtac aacgccctgc
     3841 tcgaccgcct gggctgacgg gaactcacgg cgccggcgtc cgcgtgacgg ccggcgccgt
     3901 tcgcgtgtcg ccgccacggc acggtgatgt aacccgcggg agctaaacga gcaaaacccc
     3961 ggcccccggc accccggcct ctaccttgcg gataagtgtc tttccggcac gcaggctgat
     4021 cggccgcacg aggggaggcc gcacatggac ctgttcctgc tcatcgtatc ggtggccatc
     4081 gcggcttacg ccgctgtcac cacttccgcc atcacccggg cgcacgccgc gatcaggtcg
     4141 gccgccacgg gatccgggct gggggagctc ggccactacg acctggcgta cctggtcggt
     4201 ggccccacca gggtggccga cacggccatc gccatcctcg cggagcggca cgagctccgc
     4261 gtctcgcgcg gtggacgggt gcaccgcgtc atcaccagca cgaccgcacg ggagcccatc
     4321 gaggaggcgg tgctgggcgt ggtcgccacc cgctcgggcc tgcccgtgat gtcgcttagg
     4381 atcgaggccg tcaggagcct cgccatggag gagatcaagc agcgcctcac ggcgcagggg
     4441 ctgctgctgc ccgacggggc gttcgtccag gcccgcaggc tgagcgtacg gctgcgggtg
     4501 ctgtcggtgc ccgcgtacgc cgggttcgcg atcgagggcc tgaccatggc cctggccccg
     4561 aacctgcacg ccctgctcgc cacgctcgtc ttcctgggca cgatgatcgg cgcggaggcc
     4621 gtgctcggca accaccgccg catcgtgcgg cacacgctga ccgagtccgg ccgggacgcg
     4681 ctggactcgg ccaggcgggc ccacgccccc ggcgccgacg ggccggtgcc gctcgcgctc
     4741 tacggcctcg gcgagctgcg cgacgccaag ctgcgggccg agctcagccg gcgggcgccc
     4801 cagcgggagc ggcggcccag gagctcgtac gcgggcagcg gctcgtcccg ccgcggcggc
     4861 gcctggtacg gcggcagcgg ctcgcacggc ggcggctcct acggctgcgg cggcggctcg
     4921 tcgagctgcg gaagcggatc gtcgggctgc ggcgggggcg gcggctgcgg cggcggtggc
     4981 tgcggcgggg gaagctgacc atggcgagcc tcggagtggg catcggctgg cggcccgagc
     5041 tggacctgac catcgagcgg atgggcggcg tcgacttcct cgaagtggtc gccgagaaca
     5101 tccggccggg ccgcctgccg gagtcgctgc gcgtcctgcg ggccaggggc ctgccggtca
     5161 tcccccacgg ggtctcgcta ggggtcggcg gcgcggagcc gccctccccg gcccggctgg
     5221 cccacctggc cgcgtgcgcg caggcactgg acgcgccgct ggtcagcgag cacctggcgt
     5281 tcgtccgcgg cggcggcatc gaggcggggc acctgctgcc ggtgccgcgc acgcgcgagt
     5341 cgctgaaggt cgtcatcgag aacgtgcgca gagcccagga cgcgctgccc gtcccgctgg
     5401 ccctggagaa cgtcgcggcc gtcttcggct ggcccgatga cgagctgacc gaggcgcagt
     5461 tcctggccga gctggtcgcc gccaccggcg tggggctgct gatcgacgtg gccaacctct
     5521 acaccaacca ggtcaacctg ggcctgccgg ccgtggcggc gctggacacg ctgccgctgg
     5581 agcacctggc gtacgtccac gtcgccggcg gctacgagca ccacggcatc tggcacgaca
     5641 cgcacaccac gacggccccc caggccgtcc tggacctgct gaccgagctg tgctcccggg
     5701 tcacgccgcc cggggtgctg ctggagtggg acgacgagta cccgagcgac gcggtgctgg
     5761 cggacgagct gtccaggatc agggccgcga tggcggtgcc ggggcaggcg cgatgacccc
     5821 gatggaggcg cggcggctcg ccggcgcaca ggggccgccg acgcagccgc cgcaactcac
     5881 cgccccacca ctggaagtat cggagcaggc cgcacaagag caggccgccc aagagcaggc
     5941 cgcaccagag cgcgtctcac cggaggaggc gcgggagcgg gacgaggagc ggcgcaggcg
     6001 gctggcggag gcgcaggggc gggtcgtggc cgccctggtg gcaggggcgg aggtgcccga
     6061 gggtttcgac ctggagcgca tgcgcgccca ggccgtcagc ctggtctcca agcggcgcgc
     6121 gatcgtggcg aggctccgcc cggacgcggc cgcggcggcg ggccctgacc tggccgccga
     6181 gttcgccgcg tacgcgcggg cccgcgccac gcccccgccc ggctaccgcg ccgacgccga
     6241 cgacttcgcc gcctggctgc gcgagcgcgg ccggatgccc ggcgagccgc ccccgaagcg
     6301 ccgctggtgg tcgcgcctgc ctggcatacg ctgatcccgg ccgggttcgg cagcaagcgc
     6361 ttgggaggcg gcatgcggat cgtcaacatg ggcgagtggc ccctgggccc ggaggacgag
     6421 cggcggcacg cccccggcgg cgagccgctg tggaacgagt cctggtacta cgacttcgtc
     6481 tcggaggacg gcagggcggg cggctacgtc cgcctcggcc tctacccggc gtggggccgc
     6541 gcctggtact gggcctgcct ggtcacggcc gacggcgacc gggtcgaggt gatcgaccac
     6601 gaggcggcac tgccccgggg cgacctggcg gtggcgggcc cgggctacac ggccgcgcac
     6661 gccacgacgg accccttcga gcgcgcgcac atccggctga gctccgccga gctcagcctg
     6721 gacctggagt tccgcacggc gggaggcgcg tacggctacg cgctcacccc gcgctacgag
     6781 gttccctgcg aggtcaccgg caccataggc ggcctgccct tccggggaca cggcgagcgc
     6841 gaccacagct ggggcgtgcg cgactggtgg tcgatctcgt ggttgtggag cagcgcccgc
     6901 ctggacgacg gcacgtacct gcacggcatg cgcgcgaacg tgggcatgga gctgccctgg
     6961 ccggcgttcc tggcggccaa gggggacctg gcgcatgtcg agggcttcgg ggcggccacg
     7021 gtgttcgacg gtgaccggcc ggtggagtcg gtgctgcgct tccccggctg cgtcacgacc
     7081 gtgcggccgg tcgccttcgc cccggtgacg ctgacctcgc cggagggcgt ggcggcccgg
     7141 ttcgccaggg cgctgtgcgc gtacgagacc gaggacgggc gcacgggcca cggctggacg
     7201 gagtggcacc agccaccggg ctggcgcgag cacgtgtggt agccgcgggt tcagcgagca
     7261 cgcctggtag ccgcgggttc ggcgagcacg cctgggattc acgggtttag cgcggagggg
     7321 cggggatacg ggggagggca tgggaggtct acgctgcgtc gatgggtcct atctgtccgt
     7381 tacgcccgtc gcgtgcacgg acagcgtcgg cgcgccgtac gagatcactt tggagctgca
     7441 ccgcgacggc accccgtacg gcagcgtggg tgagcgttgc ggccacctgc tggcgcgcct
     7501 ggcgcgcggc gttgatgagg cccgccaggg gcggggcgcg gccaggcgct gggccgaccc
     7561 cgacgaccgt tttcccgggg aagggcccga cggcgagctg ttctccttcc gctacctgac
     7621 cagggccggg ctgggcggcg gcgagctgcg ctgccagctg cgcaccatcc cggtctggca
     7681 gccggggccg ggggagtggc gggtgaccag gagggcgttc gtggaggcgt ggggcgccac
     7741 gggggtgggc atgcgggcgg tcctcacctc ggcggagctg tcggtgttcg tgcgcgagct
     7801 cgtggatgag gcggaagggt gtctgaaagg tagagactct gcggaggaat agtgtagacg
     7861 gagcaggcac gggcgtttcg cggtgataat tgcctgttgg ggcattgcgc caaggggcac
     7921 aagtcaccgc tggggaggca ctccgtgggc ctgcgcgatc tggtctaccg gctgtacgag
     7981 cgccggctgg aggccaagct gtcgaaggac agcatccccc ggcatgtcgg ggtcatcatc
     8041 gacggcaacc gccgctgggc gcgcgccatg ggcctgcgtg acgtgtccac aggtcaccgc
     8101 aagggcgcgg acaagatctc cgagctgctg ggctggtgcc gcgagctcgg cgtcgaggtg
     8161 gtcacggtgt ggatgttgtc caacgacaac ctcaaccggc cgagggaaga gctggagccg
     8221 ctgctgcgca tcatcgagga cgtgacgcgg gagctggtgg cggagggctg gcggatcagc
     8281 cccgtcggcg cgctcgatct gctgccggac agaaccgcca atgtcctaaa aaatgcaaaa
     8341 gaagcgtcat cacaccgtcc gggcctgatt gtgaacgttg cagttgggta tggaggaaga
     8401 cgtgagattg ccgatgcggt gcgctcactg ctccaggagc acgcgagcag gggagcgagc
     8461 atcgaggacc tcgtggaggt actcgatgtc gagcacatcg cggagcatct ttacactcgc
     8521 ggccagcccg acccggacct cctcatccgg acctcgggcg agcagcgcct gtccggtttc
     8581 atgctctggc agagtgctca ctccgagttc tacttccacg aggcctactg gccggacttc
     8641 cgcagggtcg acttcctgcg ggcgctgcgc gattacgctg cgagacaccg gcgttacggt
     8701 tcctgacacg acaatcgggt atccaggacg taacgttgga agtgtccggt cacaggtcgg
     8761 acatttcgga gagggcccgc ctttgcacca acacttgccg aggggggccg gactccggca
     8821 ccgacggcag ctcgcggtcg gggacgcggg tccggtccga ttcccacctc gtctgcaggg
     8881 tgcccgcacc tatgggcgcc cgggggagaa cgtgtggcag tgtcctcgag caaccctact
     8941 caccagtcgg ccaagcgcac gtacgtgctg gacacctcgg tcctgctagc cgacccggcg
     9001 gcgatgaccc ggttcgccga gcacgacgtg gtccttccca tcgtggtcat cacggagctg
     9061 gaaggtaagc ggcaccatcc ggagctcggc tacttcgccc gcaaggccct gcgctacctg
     9121 gacgacctgc gcgttcagta cggcaggctg gacgaggcga tgccgacggg ggacggcacg
     9181 atcagggtag agctcaacca cagcgatccg tccatcctgc ccgcgggctt ccgcctcggc
     9241 gacaacgaca cgcgcatcct gacggtggcc cgctcgctgg ccagcgaggg ctgcgacgtc
     9301 gtcctcgtct ccaaggacct gccgatgcgg gtcaaggccg cctcgatcgg cctggcggcg
     9361 gaggagtacc gcgccgagct ggtgcacgag tcaggctgga cgggcatggc cgaggtccag
     9421 gtgaccgcgg aagacgtcga gacgctgttc gagacgggca ccgcggacat ggaggaggcc
     9481 agagacctgc ccacgcacac gggcctgcgg ctgctgtcgg ccaagggctc ggcgctcggc
     9541 cgggtgcagc cggacaagtc ggtgcgcctc gtccgcggcg accgggaggt cttcggcctg
     9601 cacggccgct ccgcggagca gcgcatcgcg ctcgacctgc tgatggaccc ggagatcggc
     9661 atcgtctcgc tgggcggccg ggccggcacc ggcaagtcgg cgctggcgct ctgcgcgggc
     9721 ctggaggccg tcctggagcg ccgccagcac cgcaaggtca tcgtcttccg ccccatctac
     9781 gccgtcggcg gccaggagct cggctacctg cccggcaccg agggcgagaa gatgggcccg
     9841 tgggggcagg ccgtctacga cacgctcggc gcgatcacct cgcccgaggt gatcgaggag
     9901 gtgctcgacc gcggcatgct ggaggtgctg ccgctgacgc acatccgcgg ccggtcgctg
     9961 cacgacgcgt tcgtgatcgt ggacgaggcg cagtcgctgg agcgcggggt gctgctgacg
    10021 gtgctgagcc ggatcggcgc caactcgcgg gtggtgctca cccacgacgt ggcccagcgc
    10081 gacaacctgc gcgtggggcg gcacgacggc gtggtcgcgg tggtggagaa gctgaagggt
    10141 catccgctgt tcgcccacgt cacgctgacc aggtccgagc gctccccgat cgccgcgctg
    10201 gtgacggaga tgctggaaga catcacgatg tagtgctcag tcgagccttt gagcagtgat
    10261 gctcagttga gcaccgagca gtgatgctca gttgagctca gggcacgtgt gccgcccctc
    10321 gtccttcggg gggcggccct cgttctcccg cacgatcttg acgacctcga acgccgcgtc
    10381 ctgctgggcc gtgcagacga tctgcgccat gccgagcttg gtcagcgtgc cgtcgccgct
    10441 gatgtagacg gtcagcgtcg aggtgcgggg gagctggatc tcgtcgcgct gcggcgggtt
    10501 gatggagttc ctggtccgca ggtacgtgaa gccgcgcaac gcggtgtccc ggtcgccgag
    10561 cgggtgggcg gaggcgtcga agagcgcggt cagcacgctg tccacggtgt cgtcctcgac
    10621 gtcggcgggc tcccgcatga gcttgccgtt cctgagcagg aagatcgtct tggtgggcgg
    10681 cgggattcgg acggtggggg cgctgccccg gtcctggacg tcggtgggca ggatgccgca
    10741 gccggtcatg gccagcagca ggacgacggc gacgagagct ctcatatgtg cggcagccag
    10801 accgtgaaga gggtgc
//
