LOCUS       NZ_CP017717            20921 bp    DNA     circular CON 03-MAR-2024
DEFINITION  [Actinomadura] parvosata subsp. kistnae strain ATCC 55076
            chromosome, complete genome.
ACCESSION   NZ_CP017717
VERSION     NZ_CP017717.1
KEYWORDS    RefSeq.
SOURCE      [Actinomadura] parvosata subsp. kistnae
  ORGANISM  [Actinomadura] parvosata subsp. kistnae
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1  (bases 1 to 13047416)
  AUTHORS   Nazari,B., Forneris,C.C., Gibson,M.I., Moon,K., Schramma,K.R. and
            Setedsayamdost,M.R.
  TITLE     Nonomuraea sp. ATCC 55076 harbors the largest actinomycete
            chromosome to date and the kistamicin biosynthetic gene cluster
  JOURNAL   Medchemcomm (2017) In press
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13047416)
  AUTHORS   Nazari,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2016) Chemistry, Princeton University, 20
            Washington Road, Princeton, NJ 08544, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 18-JUN-2015
            Assembly Method                   :: Celera Assembler v. Jun 2015
            Assembly Name                     :: SmrtPipe 1.87.139483
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 13:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12,000
            CDSs (total)                      :: 11,919
            Genes (coding)                    :: 11,783
            CDSs (with protein)               :: 11,783
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 36 of 136
            Pseudo Genes (incomplete)         :: 98 of 136
            Pseudo Genes (internal stop)      :: 10 of 136
            Pseudo Genes (multiple problems)  :: 6 of 136
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:57:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 31920
            Orig. end                         :: 52841
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017717.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20921
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20921
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20921
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10921](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10921
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(783..796)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.8"
                     /tool="antismash"
     gene            818..2944
                     /locus_tag="BKM31_RS00165"
                     /old_locus_tag="BKM31_00165"
     CDS             818..2944
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893309.1"
                     /locus_tag="BKM31_RS00165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00165"
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="WP_230465915.1"
                     /transl_table=11
                     /translation="MNAPKKLLKGLLAAIVLIPVSLMATPASAAPPEDPEQPWRLNHWP
                     QTQPWQSDTPLDDAQALRTRPLSGGIKPIDPQNWENPDNMTWADYKRPPGTKWNDPSVK
                     GSQRTFKGALVLLDYPNQPFVVTQPKGSTVYGNPSAEAHDVPRDQVATFYQNFLNTPND
                     LNKGHTIHEYWMEDSGGRIGVDLTGFGPYTMPGKDHEYAMEYQRGSCPAGDTCTRNLRT
                     DGNAAWLAAVGPDVAAQFDFVFYLSAGQDESSTWQEFGMMKFPTKEDVTDEWGPPDPAL
                     PNWSPTRYVEWTSWQAGSNFWPNARFGIDSVQAESSGMAVYAHELSHIMGIADNYNNPY
                     ATPVNRAYTGIWEMLSRGSFNGPGGPHSRWLIPPTAGASMGAHHMLRNKIKLGMVDERN
                     VLRLSREALDESGLVTARVTARSVQPGENGLSGVNISFDTGDKSPACDVKTDPLCDGGG
                     WENYTVEVVDRMGSDSFTPDAGVLLAKTKNEDRGTGWQLPAFEWVIDANPQDIGMTDYV
                     LPDGTKVPITIGDYRQLSDALFHAGTDSGSEYEYVDEANRLHFYVTDVHRNRAGELSYT
                     VAIRSLDGAGPQRRGVKLLPAAGVPAAKNVSTCTFPLFNTGRAAPAQGQHPEDVSAYLK
                     SDLYRLKAEVQGKGWTAQLPNALASVAFGKQALVTVNAVRGAGGDRLAKVKLTATSESD
                     PTKTTTATCHVIGI"
     misc_feature    1016..1018
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3015..5546
                     /locus_tag="BKM31_RS00170"
                     /old_locus_tag="BKM31_00170"
     CDS             3015..5546
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /locus_tag="BKM31_RS00170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00170"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_155126261.1"
                     /transl_table=11
                     /translation="MAGDNLPDKPGRRKVPWAAPVSAGLAAAAASVLTASMEWEIPPAA
                     KIGVTLAAAVLVGLTTWATTESRGPAHERPRPEPGTMPPDQWPPVPEHFTGRAEALAEL
                     REVFAARRGSTDLSPPLVVSVYGRGGVGKSALMTRFGQEVAGWFPDGRLYADLRGGVDA
                     SVRPEEVLIGFLRALDVRLTTDPGGLEELRKLWLTWTKGRRILIGLDNAHDAEQVKPLI
                     PAEPGCAVLVTSRSPLFLNGTHDTRLGEFSEAHGVELLARLAGGARIVEDLEAAKEVVR
                     LCDYLPLAINICGGRLATREQWTMREMASRLADKRRILDLLQVAPTVDKSVRASVQLSY
                     DDCTGVQRRLLRLLSALTAPDVPGWVAGELLDVSGLDGADQLEALMDAQLAECSGTDQT
                     GTMRYRLHDLVRVFAAELAEQDEPDRRRAAIERVLHGYRRRAEEAAQNRWPQDWSRGGR
                     RSGADGQLRAGDWLNAERLSLIAMIHLAHQLRLWELTWGLARAFCSLCHSLRAYWADWA
                     VVAELGREAAEKTGDRRSLAIALLDAAAVHGGLGLRRQALEESERALAIFGELGETWWA
                     ARAMRTIGMTLFSDGHLDRAQDFLTGAITAFKGEDDLWWMARTQRNLAEMRMAERRTEE
                     ARELLEDALEVFKREGNRYSEAQTLRVYGEVLAAQARGEYRHGDHRAAANLFTRAGFSL
                     DRAGEMFKQRGELWEEARCLRAAGEVGDPDNGLRELEYVRRAEEILAALADSWGVARTA
                     VSAGRALARLGRIEEAERELRRAVRGFEELDDRWWMARSLRYLGEAHLNAGGHGAAVEP
                     LEQAREIYRSLGNEAGMRRTLELLRRAASST"
     misc_feature    4065..4067
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5539..6294)
                     /locus_tag="BKM31_RS00175"
                     /old_locus_tag="BKM31_00175"
     CDS             complement(5539..6294)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BKM31_RS00175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BKM31_00175"
                     /product="hypothetical protein"
                     /protein_id="WP_080036197.1"
                     /transl_table=11
                     /translation="MDTTRNPALLFVLPAFLMFLMMVAGPQAFFVASMGVGLIVLIRWM
                     AMDSTYRSTKRRLRLAREHANQYILPEDLDYPCQQLLRRAQNAVEAIMSSAVHKAGLID
                     SIENQVSLPEEVWQIATRLRKLSKMHAEHGKLIPRELPAGMEDAFKPYTTALDAAWTSL
                     SQRVRHLEKYAKQVLKADKVYHAHTRLEKLAAKTPEYQQLLAETVRDELAHERIRELSD
                     QAAHVRKLFEESILQARQAAGELLRSPLT"
     misc_feature    complement(6278..6294)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.6"
                     /tool="antismash"
     misc_feature    complement(6468..6481)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.6"
                     /tool="antismash"
     gene            6485..7267
                     /locus_tag="BKM31_RS00180"
                     /old_locus_tag="BKM31_00180"
     CDS             6485..7267
                     /EC_number="3.1.3.48"
                     /GO_function="GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546454.1"
                     /locus_tag="BKM31_RS00180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00180"
                     /product="tyrosine-protein phosphatase"
                     /protein_id="WP_329607687.1"
                     /transl_table=11
                     /translation="MTRWIDLEGAVNARDLGGLPTRDGGRTRRGQIFRADNLQGLTPAD
                     VELLVGELRLRHVVDLRSNAEVALEGPGPLTRVPEVRHHHLTLFAEGGRHTDVEADTID
                     GDRVLPWAERVIDEELRVTGFYFGYLRDRPDSVVAALRAMALADGASLVHCAAGKDRTG
                     VLCALALEVAGATREAIVEDYVATGERLELILKRLRGSATYRDDLDSRPADDHMPRARY
                     IEQFLGVLDDRFGGPLGWLAENGWTDADTEAMRARLRD"
     gene            complement(7388..8428)
                     /locus_tag="BKM31_RS00185"
                     /old_locus_tag="BKM31_00185"
     CDS             complement(7388..8428)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA_C3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893306.1"
                     /locus_tag="BKM31_RS00185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00185"
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="WP_080036199.1"
                     /sec_met_domain="GFO_IDH_MocA (E-value: 2.3e-26, bitscore:
                     92.2, seeds: 39, tool: rule-based-clusters)"
                     /sec_met_domain="GFO_IDH_MocA_C3 (E-value: 2e-26, bitscore:
                     91.9, seeds: 4226, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEIRVGLVGYGTAGQYFHAPLIHATPGLTLTAVVTRNPDRQAEV
                     AGRYGAAGVAEVADLWERCDLVVVASPNRTHVATATAALTQGLPVVVDKPLARTAEEGR
                     ELVRLAKERGLLLTVFHNRRWDGDFRTVRQLAGEGRLGEVLRFESRFERWRPVPKGGWR
                     ESGGPEEVGGLLYDLGSHLVDQAVQLLGPVREVYCESDVRRAGVSADDDTFIALTHASG
                     ARSHLWASSMAARLGPRFRVLGSSAGFVKYGMDVQEERLRAGMTPDSPGFGDDEEDRWG
                     VLGTENERQIVRTEPGTYLDFYRGVAAALLEGAAPPVDPGSAVDVLAIIEAARLSATQR
                     IVVRLD"
     gene            complement(8455..9357)
                     /locus_tag="BKM31_RS00190"
                     /old_locus_tag="BKM31_00190"
     CDS             complement(8455..9357)
                     /GO_process="GO:0043248 - proteasome assembly [Evidence
                     IEA]"
                     /GO_process="GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893305.1"
                     /locus_tag="BKM31_RS00190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00190"
                     /product="PAC2 family protein"
                     /protein_id="WP_080036200.1"
                     /transl_table=11
                     /translation="MFDPKDLYELADDAPELTDPVLLYHFDGFVDAGSAGRLALGHLLA
                     ELEHRVVATFDVDRLLDYRSRRPIMTFDTDRWVECESPELAVHVAKDATGTPFLLMSGP
                     EPDREWELFAKAVAALVERLKVSKLVTLHGIPMAVPHTRPLGITMHASRPELINNAQSS
                     VFGRVQVPGSVAALLELRLGAQGHDALGYAVHVPHYLSQAEYPTAAVTGLEAVTRGTGL
                     VFPMDALREAARRTTTEIEEQIRASSELSTAISGLEQQYDAFAAGAERENLMAETTPMP
                     TGDELAAQFERFLAERDDE"
     gene            9453..9845
                     /locus_tag="BKM31_RS00195"
                     /old_locus_tag="BKM31_00195"
     CDS             9453..9845
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619008.1"
                     /locus_tag="BKM31_RS00195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00195"
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="WP_080046391.1"
                     /transl_table=11
                     /translation="MDGDPELEEFATKLFGLARAGDTEALRAYVEAGVPVDLTNGKGDT
                     LLMLAAYYGHAETVRMLVTSGADPNRANDRGQTPLAGAVFKKESAVVRALLDAGADPHA
                     GQPSALETARMFANDQFIQWFTQSDS"
     misc_feature    complement(9850..9863)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.12"
                     /tool="antismash"
     misc_feature    9851..9864
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.4"
                     /tool="antismash"
     misc_feature    complement(9931..9947)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.3"
                     /tool="antismash"
     gene            10001..10921
                     /locus_tag="BKM31_RS00200"
                     /old_locus_tag="BKM31_00200"
     CDS             10001..10921
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /locus_tag="BKM31_RS00200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00200"
                     /product="hypothetical protein"
                     /protein_id="WP_155126264.1"
                     /sec_met_domain="T1TS (E-value: 2.7e-81, bitscore: 273.6,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHASEVDAESHTWLAASKMLNGADTIEHFRRSKIGLLAARTNPTV
                     PRDSLRLINDWYNWLFAFDDAFCEGELMGHRASALARALPPLLEILDGRREPDAGDPFG
                     LALHELLHRISQYATPAQVDRWRITVKEYLFAQIWEAANREADLIPTPDDYVLMRRITG
                     ATYTCFALIDAGGGYRLEAEEWHHPDVRTLSDLACDLIGWDNDLLSYAKERGNDKARHN
                     LVTVLATHKALSLQDALHEVAQMHNDAIAAFLDRRAALEQWAPLPVRKYVRGLEHWVRG
                     HIEFSLGSARYVHAWPDGTQWPQAV"
     gene            complement(10936..13056)
                     /locus_tag="BKM31_RS00205"
                     /old_locus_tag="BKM31_00205"
     CDS             complement(10936..13056)
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541908.1"
                     /locus_tag="BKM31_RS00205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00205"
                     /product="AAA family ATPase"
                     /protein_id="WP_230465916.1"
                     /transl_table=11
                     /translation="MRLLMATPTGWRREGNLPAELTSFVGRTRLLANLKRHLSESRLVT
                     VTGIGGVGKSRTVLRLAHQVRSHYPDGVWFADLARLQDAGMVKHTIASALGIADQSSRA
                     ESESLSEWVGERDMLLIIDTCEHLVQGCAELVHELLEAAPNLKVLATSRQSLHLPYEHV
                     VPVPPLQVPGDDPAESLFTNESVQLFAARAGASVPDFVLDEDNIGPVAELCRRLDGIPL
                     AIELAAVRLRALSVEQILGLLADRFSLLAGASRTALPRHQTLRAAIGWSHELCEPAERL
                     LWARLSVFAGDFELDAARFVCSDGRLPSEDITDLVTGLVEKSILLIRSNHAGVRYKLID
                     TLAEYGEEWLDKLGQKEQMSQRHLEYYLSLAQRSEEAWSGPRQIYWFVRMRQEHDNVRV
                     ALEHALKSPGKSALALTLLSSLWFMWVCCGFAREGRLYLERALAANPAVSKERCKALWV
                     LSYVKSAQGDSAGALAAAETCSTEAVRVGDSRAVILASKMQGTAALLQGDLQKASALLG
                     VAIEFNTENRELNPGLLPAIVELSIVLTTQGELYEAESLLLDCLQVCKERGELWVSSHA
                     QWALASTRLATGRTEDALQNAKEALRIKRHFHDTLGTLMALETAARIFALSGRLTLAAQ
                     LLGALQQNWKTAGLPQLGAPWMTDEHNMCVRDCRMRLGELAYKDAFEEGARLDLDEASA
                     LALDDPDALDES"
     gene            13320..13766
                     /locus_tag="BKM31_RS00210"
     CDS             13320..13766
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541906.1"
                     /locus_tag="BKM31_RS00210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_080036202.1"
                     /transl_table=11
                     /translation="MYALRPATPADYDAIAAVIDGWWGRPILPALPRLFLDHFHGTSQV
                     AETADGALAGFLIGILSPSLPDEAYIHFVGVRPDARKSGLARTMYERFFDLARADGRRV
                     VKAVTVPVNTASIAFHRRMGFEAGDPVPGYNGPGTSLVTFTRSL"
     gene            complement(13750..15426)
                     /locus_tag="BKM31_RS00215"
                     /old_locus_tag="BKM31_00215"
     CDS             complement(13750..15426)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547834.1"
                     /locus_tag="BKM31_RS00215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00215"
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="WP_080036203.1"
                     /sec_met_domain="DAO (E-value: 7.2e-55, bitscore: 186.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLTGAIDARNRQETIDRLASQTFDVLVVGGGVTGAGAALDAAAR
                     GLRVALVESRDLAAGTSSRSSKLIHGGLRYLEQFDFKLVREALKERDLLVARLAPHLVR
                     PVTFLYPLRRKVIEQPYVAAGLTLYDALEGVRRPMPRHRNLSIKEARRMAPGLRADVLT
                     GAMIYYDAQVDDARFTLTLARTAAAHGAVIATRARAVAMLRDERGERVVGARVRDEESG
                     REVDVAADAVVVCAGVWTDPVTALPGAAEAGFKVRMSKGVHVMVPGEAIDSRTGMIIRT
                     EKSVLFIIPWTSRRWIVGTTDTDYDGDRAEPAATGEDIDYLLEHANSVLARPLTRDDVV
                     GLYVGLRPLVAADETSSTTKLSREHVVDVPLPGLATIAGGKYTTYRIMAKDVIDAALEG
                     YDVPESVTERLPLLGADGLPALERSVRRIAEDHDLDVDTVAYLVRRYGNLAMEVLDLVR
                     DDPALGEPLIEPGPWLRAEVCYAVTHEGALHAEDIVARRTRMLIESSDLADQAAPVIAE
                     LMAGVLGWDRARVEEEIARCRSLVQAERTALAQARTSTTNG"
     gene            15523..16329
                     /locus_tag="BKM31_RS00220"
                     /old_locus_tag="BKM31_00220"
     CDS             15523..16329
                     /GO_function="GO:0015267 - channel activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547835.1"
                     /locus_tag="BKM31_RS00220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00220"
                     /product="MIP/aquaporin family protein"
                     /protein_id="WP_080036204.1"
                     /transl_table=11
                     /translation="MAGREARRGLTGELVAEFAGTMVLILFGAGVVAQVVAAQLGDHDS
                     IAWAWGIGVMLGVYVAGRTTGAHLNPAVTVGFALFRGFPWPKVLPYVVAQTAGAFVAAL
                     IVRWNYSEVLAMADPGRTLKTQIVFSTLPGNGTLPVDMWGGLRDQIIGTAILLFVIFAL
                     SDTANMAPSANLGPFVIGLLVVGIGMSFGTDAGYAINPARDFGPRLASFLTGYGGAWRD
                     QYGELYFWVPIVGPLIGGVLGAGLYQACIARFLPQGEDRDTVKPSD"
     gene            16522..18036
                     /gene="glpK"
                     /locus_tag="BKM31_RS00225"
                     /old_locus_tag="BKM31_00225"
     CDS             16522..18036
                     /EC_number="2.7.1.30"
                     /GO_function="GO:0004370 - glycerol kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="glpK"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1210: glycerol kinase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072696.1"
                     /locus_tag="BKM31_RS00225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00225"
                     /product="glycerol kinase GlpK"
                     /protein_id="WP_080036205.1"
                     /transl_table=11
                     /translation="MPDFVGAVDQGTTSTRFMIFDHEGAEVAKFQLEHEQILPRAGWVE
                     HNPIEIWTRTAAVIQTTLQRAGLHDSDLAALGVTNQRETTVVWNPRTGRPYSNAIVWQD
                     TRTDRIAAALEQDERGQVIRDRTGLRPDAYFSGGKIQWILENVPGVREAVDRGEAIFGN
                     TDTWTVWNLTGGVNGGVHVTDVTNASRTMLMDLDTLDWDDELLSYFGIPRAMLPEIRPS
                     SDPAGYGTTTRYGPFGGEVPITAILGDQQAATVGQVCFSPGEAKNTYGTGNFLLLNTGT
                     DRVRSQSGLLTTVAYKFGDQPAVFALEGSIAVTGSAVQWLRDQLHVITSAAQSETLARQ
                     VADSGGMYFVPAFSGLFAPYWRADARGAIVGLSRYNNDAHLARATLEAICYQSRDVVEA
                     MQRDSGVVLDVLRVDGGVTANDLCMQLQSDILGVPVSRPVVAETTALGAAYAAGLAVGY
                     WQDTDELRAHWAESRRWNPTWNEERREKAYAGWKKAVTKAFDWTTD"
     gene            complement(18270..19118)
                     /locus_tag="BKM31_RS00230"
                     /old_locus_tag="BKM31_00230"
     CDS             complement(18270..19118)
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541905.1"
                     /locus_tag="BKM31_RS00230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BKM31_00230"
                     /product="ABC transporter permease subunit"
                     /protein_id="WP_080036206.1"
                     /transl_table=11
                     /translation="MTTATGYAPNRTLPLRVEFVRQFKRRRTLGMFALLLALPWVLVIA
                     FQIGPQSSSQGQSALRMSDLATQSGLNFAAFVLSVSASFLLVVAVALFCGDTVASEANW
                     SSLRYLLAAPIPRDRLLRQKLIVALGYSAAAVIVLPLMGLLAGTLAFGWDDITVPGTGE
                     TIPAFDVLPRFGIVIGYALVSQLVVAALAFLISTMTDSPLGAVGGAVGLWIVCTILQAV
                     EALGVLREFLPTFWNNAWTDALVPELDWSGMAKGASVSVTYAAILIAIAFRRFRRKDVT
                     S"
     misc_feature    complement(19131..19147)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.15"
                     /tool="antismash"
     misc_feature    complement(19140..19154)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.85"
                     /tool="antismash"
     misc_feature    complement(20073..20088)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
ORIGIN
        1 ggccgcgtgc aggtcgtcgg cggcgaccgg cggcccggcc gtgagcagca cggcgtggtc
       61 gtagcgcacc aggtgcagcg gatggtgccc gctcagccgc cgccgcagcg cgagcagccc
      121 ctgctccagc gccagcctga gcgcgtcgtc gggctcggcc gcgcgcggcc tggccaccag
      181 cacgccgacc ggctgcgcct gcgggaacgc gccggcctcg atgagctgct cggcggcctc
      241 cctggcggcc tcgcggtcgc cgagcagcag cccggtcacc gcctccagct cgcggtgcga
      301 ggcgagcccg ctggccaggc tctcgtggaa cagggccagg gccagcccgg gcgcggcggc
      361 ggccgcctcg gcgaccccgg cgtccgtcat gggcggctgc gcgtcgatga gccacaggaa
      421 cccgagcagc ccgtccgcgt gcctgatcgg cacgcagacg cgcggcagca ggccgaggtg
      481 cggcgcgccg ggcgtgcgca ggggcgcggc ggcctcgtgg atgcccgccg cccgcagcca
      541 ctgccgcacc tcgggcgcgg tgtgccggcg caggatggag tcgcggcgca cgtcgtccat
      601 ggcgccgctc tgctcgctgt acgccacgac gcgctggacg cggtcctcca gcaggagcgg
      661 gcggtcgagc cgggcggcga gctcgtcgac ggcccgctga agatccgcca caaccccacc
      721 ttcacgtgga aatttcgtca tttgacggaa agggaactac gtgtcttccc tacaactgct
      781 cggtgatctc cgtcaataac gtctataccg tcgatccgtg aacgccccga agaagctgct
      841 gaagggtttg ctggcggcca tcgtgctgat cccggtgagc ctgatggcga cccccgcttc
      901 cgcagcaccc cccgaggacc ccgagcaacc gtggcggctg aaccactggc cccagacgca
      961 gccctggcag tcggacaccc ctctcgacga cgcccaggcc ctgcgcaccc gccccttatc
     1021 gggcggcatc aagcccatcg acccccagaa ctgggagaac cccgacaaca tgacgtgggc
     1081 ggactacaag aggccgcccg gcaccaagtg gaacgacccc tcggtcaagg gctcgcagcg
     1141 cacgttcaag ggcgcgctgg tcctgctcga ctaccccaac cagccgttcg tggtgaccca
     1201 gcccaagggc tccaccgtct acggcaaccc gagcgccgag gcccacgacg tcccccgcga
     1261 ccaggtcgcg acgttctacc agaacttcct caacaccccg aacgacctca acaaggggca
     1321 caccatccac gagtactgga tggaggactc cggcgggcgc atcggcgtgg acctgaccgg
     1381 gttcgggccg tacacgatgc ccggcaagga ccacgagtac gccatggagt accagcgcgg
     1441 ctcctgcccc gccggtgaca cctgcaccag gaacctgcgg accgacggga acgccgcctg
     1501 gctcgccgcc gtcggcccgg acgtggccgc gcagttcgac ttcgtcttct acctgagcgc
     1561 cggccaggac gagtcgtcca cgtggcagga gttcgggatg atgaagttcc ccacgaagga
     1621 ggacgtgacc gacgagtggg ggccgcccga ccccgcgctg cccaactggt cgcccacccg
     1681 ctacgtcgag tggacctcct ggcaggccgg ctccaacttc tggcccaacg cccgcttcgg
     1741 catcgactcg gtgcaggccg agagctccgg catggcggtc tacgcccacg agctgagcca
     1801 catcatgggc atcgccgaca actacaacaa cccgtacgcg acgccggtga acagggcgta
     1861 caccggcatc tgggagatgc tcagccgcgg cagcttcaac ggccccggcg gcccgcactc
     1921 ccgctggctc atcccgccca ccgcgggcgc gtccatgggc gcccaccaca tgctgcgcaa
     1981 caagatcaag ctcggcatgg tggacgagcg gaacgtgctg cgcctctccc gtgaggccct
     2041 ggacgagtcg gggctggtca cggcccgcgt cacggcccgc tcggtgcaac cgggcgagaa
     2101 cgggctgtcc ggcgtcaaca tctccttcga caccggcgac aagagccctg cctgtgacgt
     2161 caagaccgac ccgctctgcg acggcggcgg ctgggagaac tacacggtcg aggtcgtgga
     2221 ccgcatgggc agcgactcct tcaccccgga cgcgggcgtc ctgctggcca agaccaagaa
     2281 cgaggaccgc gggaccggct ggcagctccc ggcgttcgag tgggtgatcg acgccaatcc
     2341 gcaggacatc ggcatgaccg actacgtgct gcccgacggc acgaaggtgc ccatcacgat
     2401 cggcgactac cggcagctgt ccgacgccct cttccacgcc ggcaccgact ccggcagcga
     2461 gtacgagtac gtcgacgagg ccaaccgcct gcacttctac gtcacggacg tgcaccgcaa
     2521 ccgcgcgggc gagctctcct acaccgtcgc gatccgctcg ctcgacggcg cgggcccgca
     2581 gcggcgcggc gtcaagctgc tgcccgccgc cggcgtcccc gccgcgaaga acgtctccac
     2641 ctgcacgttc ccgctgttca acaccggcag ggcggccccg gcgcaggggc agcacccgga
     2701 ggacgtcagc gcgtacctga agtccgacct ctaccggctc aaggccgagg tgcagggcaa
     2761 gggctggacc gcccagctcc ccaacgccct ggcctccgtc gcgttcggca agcaggcgct
     2821 cgtgaccgtc aacgccgtgc gcggggcggg cggcgaccgg ctcgccaagg tcaagctcac
     2881 cgccacgtcg gagagcgacc cgaccaagac gacgacggcc acctgtcacg tgatcggaat
     2941 ctgagtgaac gatggggtat ggctggcgcg agtcgtaccc catcggcttg aatcaccccc
     3001 gaagggggtg ttcgttggca ggggacaacc ttccggacaa gccgggccgc agaaaggtgc
     3061 cctgggcggc acccgtctcc gccggactgg ccgccgcggc tgcgagcgtg ctgacggcct
     3121 ccatggagtg ggagatcccg cccgcggcca agatcggcgt caccctggcc gccgccgtgc
     3181 tcgtcggcct gaccacctgg gccaccaccg agtcgcgcgg ccccgcccat gagcggcccc
     3241 gcccggagcc gggcacgatg ccgcccgacc agtggccgcc cgtgcccgag cacttcaccg
     3301 gccgcgccga ggcgctggcg gagctgcgcg aggtgttcgc cgcccggcgc gggtccaccg
     3361 acctgtcacc gcctctcgtc gtctccgtgt acggccgcgg cggcgtcggc aagtcggcgc
     3421 tcatgaccag gttcgggcag gaggtggccg gctggttccc cgacggccgg ctctacgccg
     3481 acctgcgcgg cggcgtggac gcctccgtac ggcccgagga ggtgctgatc ggcttcctgc
     3541 gcgccctgga cgtgcgcctg accaccgacc ccggcgggct ggaggagctg cgcaagctct
     3601 ggctgacctg gaccaagggc cgcaggatcc tcatcggcct ggacaacgcg cacgacgccg
     3661 agcaggtcaa gccgctgatc ccggccgagc ccggctgcgc ggtgctggtc acctcgcgct
     3721 ccccgctgtt cctgaacggc acgcacgaca cccggctcgg cgagttcagc gaggcgcacg
     3781 gggtggagct gctggccagg ctggccggcg gggccaggat cgtggaggac ctggaggccg
     3841 ccaaggaggt cgtgcggctg tgcgactacc tgccgctggc gatcaacatc tgcggcggcc
     3901 ggctggccac ccgcgagcag tggacgatgc gcgagatggc gagccggctg gccgacaagc
     3961 ggcgcatcct cgacctgctc caagtggcgc ccacggtgga caagagcgtg cgcgcctccg
     4021 tccagctcag ctacgacgac tgtaccggcg tgcagcgccg cctgttacgc ctgctcagtg
     4081 cgctgaccgc gcccgacgtg cccggctggg tggccggcga gctgctcgac gtctcgggcc
     4141 tcgacggcgc cgaccagctc gaagccctca tggacgccca gctcgccgag tgctcgggca
     4201 ccgaccagac cggcacgatg cgttaccgcc tgcacgacct ggtccgggtc ttcgccgcgg
     4261 agctggccga gcaggacgag ccggaccggc gcagggccgc catcgagcgg gtgctgcacg
     4321 gctaccgccg ccgcgccgag gaggccgcgc agaaccgctg gccgcaggac tggagcaggg
     4381 gcggccggcg ctccggcgcc gacgggcagc tgcgggcggg cgactggctc aacgccgagc
     4441 ggctctccct catcgccatg atccacctgg cccaccagct ccggctgtgg gagctgacgt
     4501 gggggctggc ccgggccttc tgctcgctgt gccactcgct gcgcgcgtac tgggccgact
     4561 gggcggtcgt cgcggagctc ggccgcgagg cggccgagaa gaccggcgac cgccgctcgc
     4621 tggccatcgc gctgctcgac gccgcggccg tgcacggcgg gctgggcctg cgccggcagg
     4681 cgctggagga gtccgagcgg gccctggcga tcttcggcga gctgggggag acctggtggg
     4741 cggccagggc catgcgcacg atcggcatga ccctgttcag cgacggccac ctcgaccgcg
     4801 cccaggactt cctgaccggc gccatcaccg cgttcaaggg cgaggacgac ctgtggtgga
     4861 tggctcgcac ccagcgcaac ctggccgaga tgcggatggc ggagcgccgc accgaggagg
     4921 ccagggagct gctggaggac gccctggagg tcttcaagcg ggagggcaac cgttactccg
     4981 aggcccagac cctgcgcgtg tacggcgagg tgctggccgc ccaggcgcgc ggcgagtacc
     5041 gccacggcga ccaccgggcg gccgccaacc tgttcacccg ggccggcttc agcctcgacc
     5101 gggccggcga gatgttcaag cagcgcggcg agctgtggga ggaggcccgc tgcctgcgcg
     5161 ccgcgggcga ggtcggcgac cccgacaacg ggctgcgcga gctggagtac gtccggcggg
     5221 ccgaggagat cctggccgcg ctggccgact cgtggggggt ggccaggacg gcggtgtcgg
     5281 ccgggcgggc gctggccagg ctcgggcgga tcgaggaggc cgagcgggag ctgcggcggg
     5341 cggtgcgcgg gttcgaggag ctggacgacc gctggtggat ggcgaggagc ctgcgctacc
     5401 tcggcgaggc gcacctgaac gcgggcgggc acggggccgc ggtggagccg ctggagcagg
     5461 ccagggagat ctaccgcagc ctcgggaacg aggccggcat gcgcaggacg ctggagctcc
     5521 tgcggcgcgc ggcctcctct acgtgagcgg tgaacgcagc agttccccgg ccgcctgcct
     5581 ggcctggagg atgctctcct cgaacagctt gcgcacgtgc gccgcctggt ccgacagctc
     5641 cctgatgcgc tcgtgggcca gctcgtcccg cacggtctcg gcgaggagct gctggtactc
     5701 cggcgtcttg gccgccagct tctccagccg ggtgtgggca tggtagacct tgtccgcctt
     5761 gagcacctgc ttggcgtact tctccaggtg ccgcacgcgc tgcgacagcg acgtccaggc
     5821 ggcgtcgagc gcggtggtgt acggcttgaa cgcgtcctcc atgcccgccg gcagctcacg
     5881 ggggatgagc ttgccgtgct cggcgtgcat cttcgacagc ttccgcagcc gcgtggcgat
     5941 ctgccagacc tcctccggca gcgacacctg gttctcgata ctgtcgatca ggcccgcctt
     6001 gtgcaccgcc gagctcatga tcgcctcgac ggcgttctgc gcccggcgca ggagctgctg
     6061 gcagggatag tcgagatcct cgggcaggat gtactggttc gcgtgctcgc gcgccagccg
     6121 gagtcttcgc ttggtggatc gataggtgct gtccatcgcc atccaccgga tcaggacgat
     6181 caggccgact cccatgctcg ccacgaagaa cgcttgcggg cccgccacca tcatgaggaa
     6241 catcaggaag gcgggcagga cgaagagcag ggcgggattg cgtgtggtgt ccattcgcgc
     6301 gcccgaccga gccatgcgga gtatgtgtgg actcgaccgc agctcgaccg agatctccgg
     6361 gtgcagcgat ggatcgatca ccagccgtgg aggcagagct cgtggatcca tacgttcatg
     6421 ctgcgttatg aacctcgaat ccgctgcatg ttggcggaca tatagggtcg taataatgag
     6481 cgagatgacg aggtggatcg atctggaagg cgcggtcaac gcgcgtgacc tgggcggact
     6541 gccgactcgt gacggtggcc gcacccgtcg cgggcagatc ttccgcgccg acaacctgca
     6601 gggcctcacg ccggccgacg tggagctgct ggtgggcgag ctgagactgc ggcacgtggt
     6661 ggacctgcgc tccaacgccg aggtggcgct ggaggggccg ggcccgctga cccgggtgcc
     6721 ggaggtgcgc caccaccacc tgacgctgtt cgccgagggt ggtcggcaca ccgacgtcga
     6781 ggccgacacc atcgacggcg accgggtgct gccgtgggcg gagcgggtga tcgacgagga
     6841 gctgcgggtg accggcttct acttcggcta cctgcgcgac cgccccgact cggtggtggc
     6901 ggcgctgcgc gcgatggccc tggccgacgg ggcctcgctg gtgcactgcg cggcgggcaa
     6961 ggaccgtacc ggggtgctgt gcgcgctggc gctggaggtc gccggcgcga cccgggaggc
     7021 gatcgtcgag gactacgtgg ccacgggcga gcggctggag ctgatactca agcggctgcg
     7081 cggcagcgcc acctaccgcg acgacctcga ctcgcggccc gccgacgacc acatgccgcg
     7141 ggcccgctac atcgagcagt tcctcggcgt cctcgacgac cgcttcggcg ggccgctggg
     7201 ctggctggcc gagaacggct ggacggacgc ggacaccgag gccatgcgcg cccgcctgcg
     7261 tgactgatcg cccctgacgg acacctcgcg gacgcctctc gccaaaaggg gcacctcgcg
     7321 ttgggagggg catctcgcgt cagaagggca tctcgcgtcg gaagggcatc tcgcgtcgga
     7381 aggcgcctca gtcgaggcgg accacgatcc gctgggtcgc cgacaggcgg gccgcctcga
     7441 tgatcgccag cacgtccacc gcgctgcccg ggtcgaccgg cggcgccgcg ccctcgagca
     7501 gcgccgccgc cacgccccgg tagaagtcga ggtacgtccc cggctcggtg cgcacgatct
     7561 gccgctcgtt ctccgtgccg agcacgcccc agcggtcttc ctcgtcgtcg ccgaaccccg
     7621 gcgagtcggg cgtcatcccg gcccgcagcc gctcctcctg cacgtccatc ccgtacttca
     7681 cgaagcccgc cgaggagccc agcacccgga agcggggccc gaggcgcgcg gccatcgagc
     7741 tggcccacag gtgcgagcgg gcgccggagg cgtgggtgag ggcgatgaag gtgtcgtcgt
     7801 cggcggagac gcccgcgcgc cggacgtcgc tctcgcagta gacctcgcgc accgggccga
     7861 gcagctgcac ggcctggtcg acgaggtggc tgcccaggtc gtagagcagg ccgccgacct
     7921 cctcggggcc gccggactcg cgccagccgc ccttcggcac cggccgccag cgctcgaagc
     7981 gcgactcgaa ccgcagcacc tcgcccagcc gcccctcgcc ggccagctgc cgcacggtgc
     8041 ggaagtcgcc gtcccagcgc cggttgtgga acacggtgag caggagcccg cgctccttgg
     8101 ccagccgcac cagctcgcgg ccctcctcgg cggtgcgcgc caggggtttg tccacgacga
     8161 cgggcaggcc ctgcgtgagc gcggcggtcg cggtggccac gtgcgtcctg ttgggcgagg
     8221 cgaccacgac gaggtcgcac cgctcccaca ggtccgccac ctcggccacc cccgccgcgc
     8281 cgtacctgcc ggcgacctcg gcctggcgat ccgggttgcg cgtgacgacg gccgtcaggg
     8341 tcaggccggg ggtggcgtgg atcagtgggg catggaagta ctgccccgcg gtgccgtaac
     8401 cgacgaggcc gacccggatc tcactcatcg ggccagcgta gcgggggtgc gggcctattc
     8461 gtcgtcacgc tcggcgagga agcgctcgaa ctgggcggcg agctcgtcgc cggtgggcat
     8521 gggcgtggtc tcggccatca ggttctcgcg ttccgcgccc gcggcgaacg cgtcgtactg
     8581 ctgctccagg ccgctgatcg cggtggacag ctcgctggag gcgcggatct gctcctcgat
     8641 ctccgtcgtc gtacggcggg cggcctcgcg cagcgcgtcc atcgggaaca ccagccccgt
     8701 gccccgcgtc acggcctcca gcccggtcac ggcggcggtc gggtattcgg cctgcgacag
     8761 gtagtgcggc acgtgcaccg cgtaccccag cgcgtcgtgg ccctgcgcgc cgagccgcag
     8821 ctccagcagc gccgcgacgc tgccgggcac ctggacacgc ccgaacacgc tgctttgcgc
     8881 attgttgatc agctccggcc tgctggcgtg catggtgatg ccgagcgggc gggtgtgcgg
     8941 caccgccatg gggatgccgt gcaacgtcac cagcttgctc accttcagcc gctccaccaa
     9001 agccgccacc gccttggcga acagctccca ctcccggtcg ggctcggggc cgctcatcaa
     9061 caggaacggt gtgccggtcg cgtccttcgc gacgtgcacg gccagctcgg ggctctcgca
     9121 ctccacccac cggtcggtgt cgaacgtcat gatgggccgc cgcgaccggt agtcgagcag
     9181 ccggtcgacg tcgaatgtcg ccacgacccg gtgctccagc tcggccagca ggtggcccag
     9241 cgcgagccgt ccggccgagc ccgcgtcgac gaagccgtcg aagtggtaca gcaacaccgg
     9301 atcggtaagc tcgggcgcgt cgtctgcgag ctcgtagaga tccttcgggt cgaacacggt
     9361 tgaacagcct ttccctaggt actcttcacc agaacctacg gcgcgtcgta tcgattccgt
     9421 gcacggcacc ctatcgaggc acaatgggca tcatggacgg ggatccggag ctggaagagt
     9481 tcgcgaccaa gctgttcggc ctggccaggg cgggtgacac cgaggcgctg cgggcgtatg
     9541 tggaggccgg ggtgccggtc gacctgacca acggcaaagg tgacactttg ctgatgctgg
     9601 cggcttatta cggtcacgcc gagactgttc ggatgcttgt cacaagcggc gccgatccca
     9661 atcgtgccaa cgaccgtggg cagacgccgc tcgcgggcgc ggtcttcaag aaggagtctg
     9721 ccgtggtcag ggcgcttctg gacgccgggg ccgacccgca tgccggtcag ccgtccgccc
     9781 ttgagaccgc gcgaatgttc gcgaatgacc agtttatcca gtggttcacg caaagtgatt
     9841 catagtatga aataagcatt atagggctcg aagttgccga gtgtggcctt tcggtcactc
     9901 catcgtcatg ctgggggacg tggctacttc tgcgcactcc ttcagcaggc gaccgctcca
     9961 catccctccg ttgccatgcc cgttccccaa cgagatcaac gtgcatgcct cagaggtcga
    10021 tgcggagagc cacacctggc tggccgcctc aaagatgctc aacggcgccg acaccatcga
    10081 gcacttccgg cgctcgaaga tcggcctgct cgcggcacgg accaacccca ccgtgccgcg
    10141 cgacagtctc cggctgatca acgactggta caactggctc ttcgccttcg acgacgcgtt
    10201 ctgcgaaggc gagctgatgg ggcaccgggc ctcggccctg gcccgtgccc tccccccgct
    10261 cctggagatc ctcgacgggc gcagggagcc cgacgccggc gacccgttcg gcctcgcgct
    10321 ccacgagctg ctgcaccgca tctcgcagta cgccaccccc gcacaggtgg accggtggcg
    10381 catcacggtg aaggaatacc tgttcgccca gatctgggag gccgccaacc gcgaggccga
    10441 cctcatcccc acgcccgacg actacgtcct gatgcgccgc atcaccggtg ccacctacac
    10501 ctgcttcgcc ctcatcgacg cgggcggcgg ctaccgcctc gaagccgagg agtggcacca
    10561 ccccgacgtc cgcacgctct ccgacctggc gtgcgacctc atcggctggg acaacgacct
    10621 gctgtcgtac gccaaggaac gcggcaacga caaggcgcgg cacaacctcg tcacggtgct
    10681 ggccacgcac aaggccctgt ccctgcagga cgcgctgcac gaggtcgcgc agatgcacaa
    10741 cgacgccatc gccgcgttcc tcgaccggcg cgcggcactg gagcaatggg ctccgctccc
    10801 ggtccgcaag tacgtacgtg gcctcgagca ctgggtgcgc ggccacatag agttctccct
    10861 tggcagtgcg cgttacgtgc acgcctggcc tgacggcacc cagtggccgc aggccgtctg
    10921 acggtcccgc ttcccttagc tctcgtcgag ggcgtccggg tcgtccagcg cgagcgcgga
    10981 cgcctcgtcg agatcgagcc gggcgccctc ctcgaaggcg tccttgtagg ccagctcgcc
    11041 cagccgcatc cggcagtcac gcacgcacat gttgtgctcg tcggtcatcc agggcgcgcc
    11101 gagctgcggc agccccgccg tcttccagtt ctgctgcagc gccccgagga gctgggcggc
    11161 cagcgtgagc ctgccggaca gggcgaagat ccgcgccgcg gtctccagcg ccatcagcgt
    11221 gccgagcgtg tcgtggaagt gccgcttgat gcgcagcgcc tccttggcgt tctgcagcgc
    11281 gtcctcggtg cggccggtcg cgagccgggt gctggccagc gcccactgcg cgtgggagct
    11341 gacccacagc tcgccgcgct ccttgcacac ctggaggcag tcgagcagca gcgactcggc
    11401 ttcgtacagc tcgccctgcg tggtcagcac gatcgacagc tcgacgatcg cgggcagcag
    11461 gcccgggttg agctccctgt tctcggtgtt gaactcgatc gccacgccga gcagcgcgct
    11521 ggccttctgc aggtcgccct gcagcagcgc ggccgtgccc tgcatcttcg aggccaggat
    11581 gaccgcccgc gagtcgccca cccggaccgc ctcggtgctg cacgtctcgg ccgccgccag
    11641 cgctcccgcg ctgtcgccct gcgcgctctt gacgtacgac agcacccaca gcgccttgca
    11701 gcgctccttg ctcaccgccg ggttggccgc cagcgcccgc tccaggtaca ggcggccctc
    11761 gcgggcgaac ccgcagcaca cccacatgaa ccacagcgac gacagcagcg tcagcgccag
    11821 cgccgacttg cccggcgact tcagcgcgtg ctccagcgcg acccgcacgt tgtcgtgctc
    11881 ctgccgcatc cggacgaacc agtagatctg ccgcggcccc gaccaggcct cctcgctgcg
    11941 ctgggccagg ctcaggtagt actccaggtg ccgctgcgac atctgctcct tctggccgag
    12001 cttgtcgagc cactcctcgc cgtactcggc cagggtgtcg atcagcttgt agcgcacgcc
    12061 cgcgtggttg ctcctgatca gcaggatcga cttctccacc agccccgtga ccaggtcggt
    12121 gatgtcttcg gacggcagcc gcccgtcgga gcagacgaac cgggccgcgt cgagctcgaa
    12181 gtcgccggcg aacaccgaca gccgcgccca cagcagccgc tcggccggct cgcacagctc
    12241 gtggctccag ccgatcgccg cccgcaacgt ctggtggcgc ggcagcgccg tgcggctcgc
    12301 gcccgccagc aggctgaacc ggtcggccag cagcccgagg atctgctcca ccgacagcgc
    12361 ccgcagccgc accgcggcca gctcgatcgc cagggggatg ccgtcgagcc tgcggcacag
    12421 ctccgcgacc ggcccgatgt tgtcctcgtc cagcacgaag tcgggcacgc tcgccccggc
    12481 cctggccgcg aacagctgca ccgactcgtt cgtgaacagg ctctccgccg ggtcgtcgcc
    12541 cggcacctgc aacggcggca ccggcaccac gtgctcgtac ggcaggtgca gcgactggcg
    12601 gctggtcgcc agcaccttca ggttgggcgc cgcctccagc agctcgtgca ccagctcggc
    12661 gcagccctgc accaggtgct cgcaggtgtc gatgatgagc agcatgtcgc gctcgcccac
    12721 ccactccgac agcgactccg actccgcccg cgacgactgg tcggcgatgc cgagcgccga
    12781 ggcgatcgtg tgcttgacca tgccggcgtc ctgcagccgg gccaggtcgg cgaaccacac
    12841 gccgtcggga taatgggagc gcacctggtg cgccagccgc agcacggtgc gcgacttgcc
    12901 cacaccgccg atgccggtca ccgtcaccag ccgcgactcg ctgaggtgtc gtttgaggtt
    12961 ggcgaggagg cgggtacggc cgacgaagct ggtcagctcc gccggcagat tgccttcacg
    13021 ccgccagcct gtcggggtgg ccatgagcag cctcacgggg ggtaggcgac acctgccttc
    13081 gcatcgtacc gccgtggtcg ccgcctgtcc ctggaccgaa ggacgcgccc ggtgccggtt
    13141 tgtacgcctg agtagcatgg acgtcgtgga ggattttcta cctgagcaga ccgccgacga
    13201 caccgatctc ggctggggcg actggcgcga ggccgactcc gacgccttct acctcgacaa
    13261 ccgcccgccc cactggggct agcaccccct tccgtgcacg ggggctagca tccccttcca
    13321 tgtacgctct gcgccccgcc acgcccgccg actacgacgc catcgccgcc gtgatcgacg
    13381 gctggtgggg ccgcccgatc ctgcccgccc tgccccggct cttcctcgac cacttccacg
    13441 gcaccagcca ggtcgccgag acggctgacg gcgctctggc gggcttcctg atcggcatcc
    13501 tgtcgccgtc gctgcccgat gaggcgtaca tccacttcgt cggcgtccgc ccggacgcgc
    13561 ggaagagcgg gctggcccgc accatgtacg agcgcttctt cgacctggcc cgcgctgacg
    13621 ggcggcgggt cgtcaaggcg gtcacggtgc cggtcaacac ggcctccatc gccttccacc
    13681 gccgcatggg cttcgaggcc ggcgaccccg tccccggcta caacggtccc ggcacgagcc
    13741 tggtcacgtt cacccgttcg ttgtagaggt gcgggcctgg gccagggcgg tgcgttcggc
    13801 ctgcaccagg ctccggcagc gggcgatctc ctcctccacc cgcgcccgat cccaccccag
    13861 cacccccgcc atcagctccg cgatcaccgg agccgcctgg tcggccaggt cgctcgactc
    13921 gatgagcatc cgcgtccggc gggccacgat gtcctcggcg tgcagcgcgc cctcgtgcgt
    13981 gaccgcgtag cagacctcgg cccgcagcca cggccccggc tcgatcagcg gctcgcccag
    14041 cgcagggtca tcgcgcacca ggtcgagcac ctccatggcc aggttcccgt agcggcgcac
    14101 caggtaggcg acggtgtcca cgtcgaggtc gtggtcctcc gcgatgcggc gcaccgagcg
    14161 ttcgagcgcg ggcagcccgt cggcgccgag cagcggcagc cgctcggtca ccgactccgg
    14221 cacgtcgtag ccctccagcg ccgcgtcgat cacgtccttc gccatgatcc ggtacgtggt
    14281 gtacttgccg cccgcgatcg tggccaggcc gggcagcggc acgtccacga cgtgctcgcg
    14341 cgacagcttc gtggtggagg aggtctcgtc tgcggccacc agcggccgca gccccacgta
    14401 gaggcccacc acgtcgtccc tggtcagcgg cctggccagg acggagttgg cgtgctccag
    14461 gaggtagtcg atgtcctcgc cggtcgccgc cggctcggcg cggtcgccgt cgtagtcggt
    14521 gtcggtcgtg ccgacgatcc agcgccggct ggtccacggg atgatgaaca acacgctctt
    14581 ctccgtacgg atgatcatgc cggtccgcga gtcgatggcc tcgcccggca ccatgacgtg
    14641 cacgcccttc gacatccgta ccttgaagcc cgcctccgcc gcccccggca gcgcggtcac
    14701 cggatccgtc cacacgccgg cgcacaccac caccgcgtcg gcggcgacgt ccacctcgcg
    14761 gccgctctcc tcgtcgcgga cccgcgcgcc caccacccgc tcgccgcgct cgtcccgcag
    14821 catcgccacc gcccgcgccc gcgtcgcgat cacggcgccg tgcgcggcgg ccgtgcgggc
    14881 cagggtgagc gtgaagcggg cgtcgtccac ctgcgcgtcg tagtagatca tcgccccggt
    14941 cagcacgtcg gcgcgcaggc cgggcgccat ccggcgggcc tccttgatcg acaggttgcg
    15001 gtgcctcggc atcgggcggc gcacgccctc cagcgcgtcg tacagcgtca gcccggccgc
    15061 cacgtacggc tgctcgatca ccttgcggcg cagcgggtac aggaacgtga ccggcctgac
    15121 caggtgcggc gccagccggg cgacgagcag gtcccgctcc ttcaacgcct ccctgaccag
    15181 cttgaagtcg aactgctcca gataccgcag cccgccgtgg atgagcttgc tggacctgct
    15241 cgacgtgccc gccgcgaggt cgcggctctc cacgagcgcc acccgcaacc cgcgcgccgc
    15301 cgcgtccaga gcggcgccgg cccccgtcac gccaccgccg acgaccagca cgtcgaacgt
    15361 ctggctcgcg agccggtcga tggtctcctg acggttcctg gcgtcgatcg ccccggtcag
    15421 ccccatggca catccatcac tttccgcaac gtatgacaca cagagcgtgt tcgttccctc
    15481 gaacggggaa tggcacacaa cgccgcttca aggaggtgac cggtggccgg acgcgaagcc
    15541 cggagagggc tgacggggga gctcgtcgcc gagttcgcgg gaacgatggt gctcatcctg
    15601 ttcggcgccg gtgtcgtcgc ccaggtcgtc gccgcccagc tcggcgacca cgacagcatc
    15661 gcctgggcct ggggcatcgg cgtgatgctc ggcgtctacg tcgccgggcg cactacgggc
    15721 gcgcacctca accccgccgt caccgtcggg ttcgccctgt tccgcggctt cccctggccc
    15781 aaggtcctgc cgtacgtcgt cgcgcagacc gccggagcct tcgtcgccgc cctcatcgtg
    15841 cgctggaact actccgaggt gctggccatg gccgaccccg gccgcaccct caagacccag
    15901 atcgtcttct ccaccctgcc cggcaacggc accctgcccg tcgacatgtg gggcgggctg
    15961 cgcgaccaga tcatcggcac cgcgatcctg ctcttcgtca tcttcgccct gtccgacacg
    16021 gccaacatgg caccgagcgc caacctcggg ccgttcgtca tcggcctgct cgtcgtcggc
    16081 atcggcatgt ccttcggcac cgacgccggc tacgccatca acccggcccg cgacttcggc
    16141 ccccgcctgg ccagcttcct caccggctac ggcggcgcct ggcgcgacca gtacggcgag
    16201 ctgtacttct gggtgccgat cgtcggcccg ctgatcggcg gcgtcctcgg cgcgggcctc
    16261 taccaggcgt gcatcgcccg cttcctcccc cagggcgagg accgcgacac cgtgaagccg
    16321 tccgactgac ccagagcccg accggccagg gctggaccga ccagagctga accgaccggg
    16381 gtggaccgac cagagctgaa ccgaccgggg tggaccgacc ggggctggac cgaccgacca
    16441 gagcccgacc gaccagagcc cgaccgacca gagcccgacc gaccagggtt cgattgaccg
    16501 atcaggggag agcagagcac catgccggac ttcgtcggcg ccgtcgacca gggcaccacc
    16561 agcacccgct tcatgatctt cgaccatgag ggcgccgagg tcgccaagtt ccagctcgaa
    16621 cacgagcaga tcctgcccag ggccggctgg gtcgagcaca accccatcga gatctggacc
    16681 aggaccgccg ccgtcatcca gaccaccctg caacgcgccg gcctgcacga cagcgacctc
    16741 gccgccctcg gcgtcaccaa ccagcgcgag accaccgtcg tgtggaaccc gcgcaccggc
    16801 cgcccgtact ccaacgccat cgtctggcag gacacccgca ccgaccgcat cgccgccgcc
    16861 ctcgaacagg acgaacgcgg ccaggtcatc cgcgacagga ccggcctgcg ccccgacgcc
    16921 tacttctccg gcggcaagat ccagtggatt ctcgagaacg tccccggcgt ccgcgaggcc
    16981 gtggaccgcg gcgaggcgat cttcggcaac accgacacct ggaccgtgtg gaacctcacc
    17041 ggcggcgtca acggcggcgt gcacgtcacc gacgtcacca acgccagccg caccatgctg
    17101 atggacctcg acacgctcga ctgggacgac gagctgctgt cgtacttcgg catcccccgc
    17161 gccatgctcc ccgagatccg cccctcctcc gaccccgccg gctacggcac caccacccgg
    17221 tacgggccgt tcggcggcga agtgccgatc accgcgatcc tcggcgacca gcaggccgcc
    17281 accgtcggcc aggtgtgctt ctcacccggc gaggccaaga acacctacgg caccggcaac
    17341 ttcctgctgc tcaacaccgg caccgaccgc gtccgctccc agagcggcct gctcaccacc
    17401 gtcgcctaca agttcggcga ccagcccgcc gtcttcgccc tggaaggctc gatcgccgtc
    17461 accggctccg ccgtccagtg gctgcgcgac cagctccacg tcatcacctc cgcggcccag
    17521 agcgagacgc tcgcccgtca ggtcgccgac tccggcggca tgtacttcgt gcccgccttc
    17581 tccggcctgt tcgcccccta ctggcgggcc gacgcccgcg gcgccatcgt cggcctgtcc
    17641 cgctacaaca acgacgccca cctcgcccgc gccaccctcg aagccatctg ctaccagagc
    17701 cgcgacgtcg tcgaggccat gcagcgggac tccggcgtcg tgctcgacgt cctccgcgtg
    17761 gacggcggcg tcaccgccaa cgacctgtgc atgcagctcc agtccgacat cctcggcgtc
    17821 cccgtcagcc gccccgtcgt cgccgagacc accgcactcg gcgccgccta cgccgccggc
    17881 ctcgccgtcg gctactggca ggacaccgac gagctgcgcg cgcactgggc ggagtcgcgc
    17941 cgctggaacc cgacgtggaa cgaggagcgg cgcgagaagg cgtacgccgg ctggaagaag
    18001 gccgtcacca aagccttcga ctggaccacc gactgaccac ccacccgcag caccacccca
    18061 tccccccacc accggccccg cagccgcctt tctccagctc ctgacgccct cccgacggac
    18121 cctgcagccg ccttccttca gctcccggcg ccctcccgac ggagccgcag ccgccctctt
    18181 ccagcctccg acgccctcac gatgggacgc tgggaaggcg gcccccggct ggtcaggtcc
    18241 ttgcggcgga accgcgggat cgaccgcctt cagctggtga cgtccttgcg gcggaagcgg
    18301 cggaacgcga tcgcgatcag gatcgccgcg tacgtgaccg acaccgacgc gcccttcgcc
    18361 atcccgctcc agtccagctc cggcaccagg gcgtcggtcc aggcgttgtt ccagaacgtc
    18421 ggcaggaact cgcgcagcac ccccagcgcc tccaccgcct gcaagatcgt gcagacgatc
    18481 cagagcccga ccgccccgcc caccgcgccg agcggggagt cggtcatggt ggagatgagg
    18541 aaggccagag ccgccaccac gagctggctg accagcgcgt acccgatcac gatcccgaag
    18601 cgcggcagca cgtcgaacgc cgggatggtc tcgccggtgc cgggcaccgt gatgtcgtcc
    18661 cagccgaacg ccagcgtgcc cgcgagcagc cccatcagcg gcagcacgat caccgccgcc
    18721 gccgagtagc cgagcgccac gatcagcttc tgccgcagca gccggtcacg cgggatcggc
    18781 gctgccagca ggtagcgcag ggacgaccag ttggcctcgc tcgccaccgt gtcgccgcag
    18841 aacaacgcga ccgccaccac gagcaggaaa ctggccgaca ccgacaggac gaacgccgcg
    18901 aagttcagcc cgctctgcgt cgccagatcc gacatgcgca gcgccgactg cccctgtgag
    18961 cttgactgcg gcccgatctg gaacgcgatc acgagcaccc acggcagcgc cagcagcaac
    19021 gcgaacatgc ccagcgtccg gcgccgcttg aactgccgga cgaactccac ccgcaacggc
    19081 agcgtgcggt tcggcgcgta gccggtcgcg gtggtcatgg cttgtctccg atgagatcga
    19141 ggaacacgtc ttccaggcgc ggcccgtgcc cgttcctgac ggccgccgcc gagcccagca
    19201 gccgcgacac gggccccgcc gacaccagcc gcccgcgccg catgaccacc acgtgactgc
    19261 aggtctgctc cacctcggcc agcatgtggc tggacacgat cacggtacga ccgtcgcgcg
    19321 cataccgctt gagcacctcc cgcatctccc ggatctgggg cgggtcgagc ccgttggtgg
    19381 gctcgtccag cacgagcagg tccggcaggc cgagcatcgc ctgcgcgatg gccagccgct
    19441 ggcgcatgcc ctgcgagtac gtacgcaccg cccgctccag cgccttgccc aggcccgcga
    19501 tctccagagc ctcctcgaag tgcgcgtccg ccttgggccg gccggtggcg gcccagtaca
    19561 gctcgatgtt gtcccgcccg gacaggtgcg gcaggaaccc gggcccctcc acgaacgacc
    19621 cgagcctcga cagcacgggc gcccccgggc tgacccgctc cccgaagatc ctgatctcac
    19681 cggcgtccgg atggatgagc cccatcatca tccgcatcgt ggtcgtctta cccgccccgt
    19741 tgggaccgag cagcccgagc acctgccccc gctccacccg gaacgacagg tcgttcacgg
    19801 ccagctcccc gttccggtac gccttcgtga gcccgctgat ctccagcggc acccccgcac
    19861 cgtcgccagc cgcctcctcg aagcggacag caccgccccg caccccgccg ccggccaccg
    19921 cggcgacacc actgccgccg ccgataccgg caccggcctc gctccagcca tcaccaccga
    19981 caccattgct cgtgctgcca gcgcctgcgc catcgccatg gccggcgtcc ccgtcagcgc
    20041 cacggccacc gtcggccctg ccatcgtcgg cagtgacgtc ggcgtgacca ccgttcgcat
    20101 cgccaccgcc gggacgtgcg gtgccgtcgt gccccgcccc gctcccagcg tcggcgtcat
    20161 acgctgcacc gttcgtgggg aaggagcctg ttgccccgtt cgtggcgtcg gcgttggcgt
    20221 cgtgcttggc gctgctggcg gaagcgtcct gtggcgtgcc gttcagggcg ggtgcggcga
    20281 gaggtgcgtc cggtgtggcc gacggcgcgg ccccgccctg gccggctgtc gtgaggacag
    20341 gacggcggcg ggagcggccc gtggacagga ggacggcggc gagcagcacg gcggcgagcg
    20401 gcatcgccca cacccaccac gccacccccg tgggagcggc ggcgagcgtg ctcaccgtgg
    20461 ggatggtgac ggcgggggag gccagcgcga cctggtacgc ggccggctgg gcgggcgtgg
    20521 cgaatcccat gtcggtggtg gtgacgacca ggcggagccg gtgcccgacg gcgaagcggt
    20581 ggtcgacggc gggcaggctg atcgtggcct ccgcgctgcc cgcgtcctcc gggatcgtca
    20641 cccggacggg cgccaccaga ccggcgggca gggtgggcag ctgggtgtcg tcggcgacgt
    20701 cgtacagctt cgcgaacagg gtggcctcgc cctcgcccgt cacccggacc cggacggtgg
    20761 ggctgccggt gagctggagc ggggcggtga gcggcgcgga ctcgaacgcg gcgctctggc
    20821 ccggcatgtc gatggacacg ccgccggtgt tctggccgcc gagcagcccg ccgaggccgg
    20881 gcacggtgga gaccgaggcc ggggcgccgc cgggcgggtt g
//
