LOCUS       NZ_CP020039            11350 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. 3211 isolate 3 chromosome, complete genome.
ACCESSION   NZ_CP020039
VERSION     NZ_CP020039.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 3211
  ORGANISM  Streptomyces sp. 3211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Complete genomes of three Streptomyces isolated from disease
            suppressive soils
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2017) Biotechnology Institute, University of
            Minnesota, 1479 Gortner Ave, Saint Paul, MN 55108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JAN-2017
            Assembly Method                   :: PacBio v. Jan-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:18:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,254
            CDSs (total)                      :: 8,154
            Genes (coding)                    :: 7,815
            CDSs (with protein)               :: 7,815
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 206 of 340
            Pseudo Genes (incomplete)         :: 257 of 340
            Pseudo Genes (internal stop)      :: 68 of 340
            Pseudo Genes (multiple problems)  :: 153 of 340
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:04:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6346392
            Orig. end                         :: 6357742
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Michael Smanski.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11350
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11350
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11350
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6350](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6350)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    331..346
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.12"
                     /tool="antismash"
     misc_feature    331..346
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.33"
                     /tool="antismash"
     misc_feature    complement(767..781)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     gene            1312..2025
                     /gene="hemQ"
                     /locus_tag="B4U39_RS29230"
     CDS             1312..2025
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072526.1"
                     /locus_tag="B4U39_RS29230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_053177202.1"
                     /transl_table=11
                     /translation="MTAPEKIPNAGKKAKDLNEVIRYTLWSVFKLKDVLPEDRTGYADE
                     VQELFDQLGAKDITVRGTYDVSGLRADADVMIWWHAETADELQTAYNLFRRTKLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADEVARDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWMLAFEADELYRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKSVADLVAGLA"
     gene            complement(2156..3823)
                     /locus_tag="B4U39_RS29235"
     CDS             complement(2156..3823)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (118-328). E-value:
                     2.3e-18. Score: 58.6. Matches aSDomain:
                     nrpspksdomains_B4U39_RS29235_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380016.1"
                     /locus_tag="B4U39_RS29235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_079406438.1"
                     /sec_met_domain="PF00561 (E-value: 2.2e-23, bitscore: 82.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTGLAGRTAAVAALALLLCAPAHLPAHALAGTPPGSPGGTDAEA
                     AEAAGAALAARRAAARGGGLDFGRCPDVEGLSGPVRCATLRVPLDYARPDGPQISLTVS
                     RTAATGARGAVRQGALIHNPGGPGASGMHFPLVADLPGWESIAAAYDLVGYAPRGVGRS
                     APLSCQDPATRAAGPTQVPAEPSPAYKRQRLAAARAYARGCSRRAGAALPYYTTLDNAR
                     DLDVLRAALGERKLSFMGASYGTYLGAVYATLYPGRVRRMVLDSAVDPDPRRIWYRNNL
                     DQSAGFERRWYDFRAWAARHHDTYGLGATPAAVQASYERVRDAVARTPAGGVVGTGELR
                     AAYLQAAYYDDVWPDRAAALAAHLRGDPAPLTAQAGPAADPAAAAEAENATAVYTAVLC
                     NDAPWPSDWETWDRDHTELARRAPFETWANAFLNLPCASWPVRERQRPVAVGNGPQRLP
                     ATLVVAAERDGATPYPGALELQRRLGAGASLVTEAGAGSHGVVGGRNDCVDRHVERYLL
                     TGATPGWRVTCAPHPEPAPVSLDDRAAGARRALLPPVV"
     aSDomain        complement(2840..3469)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4U39_RS29235_Abhydrolase_1.1"
                     /evalue="2.30E-18"
                     /label="B4U39_RS29235_Abhydrolase_1.1"
                     /locus_tag="B4U39_RS29235"
                     /protein_end="328"
                     /protein_start="118"
                     /score="58.6"
                     /tool="antismash"
                     /translation="ALIHNPGGPGASGMHFPLVADLPGWESIAAAYDLVGYAPRGVGRS
                     APLSCQDPATRAAGPTQVPAEPSPAYKRQRLAAARAYARGCSRRAGAALPYYTTLDNAR
                     DLDVLRAALGERKLSFMGASYGTYLGAVYATLYPGRVRRMVLDSAVDPDPRRIWYRNNL
                     DQSAGFERRWYDFRAWAARHHDTYGLGATPAAVQASYERVRDAVART"
     gene            complement(3958..5004)
                     /locus_tag="B4U39_RS29240"
     CDS             complement(3958..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956906.1"
                     /locus_tag="B4U39_RS29240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_107470228.1"
                     /transl_table=11
                     /translation="MNLLAVAIWIAVLVSSALLITGLRRARSAPAGPAAGLHDLSEAAF
                     VVGGPGTVVDAALVSMLGDGRLVAGGPGIVQVRPGARAADPAERAVLQAHRGAPSGWLY
                     QVRYTAMCDPAVQEIGDALAARGLITPPGSGLRRFRRWGLIQALVCALLFPVSMVLTFV
                     ALAVDSGSAVPFVMKVIFALVGGIVVGLVCAAGAKSRVTGAGARALRAVRSAHLNDRTP
                     HVQTALFGLRGLRDPYLRQQLVPAARGTRLAAAQSRTGSHGTGGSAHWSGAEVLPIVWC
                     AGSDGGSGGGSSCGSGSGCGGSTGCGSSGGGCGSSGGGSSCSSSSSSCGSSSSCGSSSS
                     SSCGSSSS"
     gene            complement(5001..6350)
                     /locus_tag="B4U39_RS29245"
     CDS             complement(5001..6350)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888544.1"
                     /locus_tag="B4U39_RS29245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_079406440.1"
                     /sec_met_domain="DUF692 (E-value: 1.7e-89, bitscore: 298.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPMAHLGVGIGWRPEIADAVERLPGLDWVEVVAENVCPGHLPES
                     LLRLRERGTRVVPHGVSLGLGGADRPDPAKLAALGERAVALGAPLVTEHIAFVRTSSPV
                     LEAGHLLPVPRTRDALDVLCENVRIAQDSLPVPLALENIAALFSWPGEELTEAQFLTEL
                     VERTGVRLLIDVANLHTNRVNRGEDPAAVLDAIPLEALAYVHVAGGIERGGVWHDTHAH
                     PVPPVVLDLLAALRSRVDPPGVLLERDDDFPAEAELAGELAAIRAVVTAGPAAVADRAL
                     AGSGPAAAEPRPDAEPDAAAERDAADEPRPVEESARTRVALGQAALLSALVAGTPVPEG
                     FDRRRIRVQARALAAKRADVVARLAPELPEILGGPDPYREAFLSYAKGRPMTAGYRRDA
                     LDFAEDLLIRDLPADPVARRRLTAWWRDRTGARPPRRIVRWARALAGRAA"
     misc_feature    6341..6355
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 15.61"
                     /tool="antismash"
     gene            complement(6484..7236)
                     /locus_tag="B4U39_RS29250"
     CDS             complement(6484..7236)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267202.1"
                     /locus_tag="B4U39_RS29250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_079406441.1"
                     /transl_table=11
                     /translation="MSSQHTNDISDVPAVGEDSPFRQEHVRRDEAPQFVLPLVVRIEKA
                     EPPARTDALETAARAVLVLLTDERAQGDGEWAGAVRDWQDARIRKVVRRARGAEWRKAE
                     TLPGVTVDGDGAQVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPVAAVTEPGLPVLWLN
                     PGLDMSAGKAMAQAGHAAQLAWWELTGAERAAWQESGFRLAVRTAPRERWAELGGSGLP
                     VVRDAGFTEIAPGSATVVADHPALRARL"
     gene            7308..7982
                     /locus_tag="B4U39_RS29255"
     CDS             7308..7982
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153329.1"
                     /locus_tag="B4U39_RS29255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AIM24 family protein"
                     /protein_id="WP_030386071.1"
                     /transl_table=11
                     /translation="MKSDLFASENLARTAVAPGMSLQNAKSVKYNVNGEMLARQGSMVA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMSVRGQGEAWFAHQAGNCFIIDFEPGDALT
                     INGRNVLCFDPTLSYEIKMVKGAGMTGGGLFNSLFTGTGKLAVVCDGHPIIIPVTPQAP
                     VHVDTDAVVGWSAQLETGLHRSQSVGSMIRGGSGEAVQLKLSGEGFVIVRPSEVTETAA
                     GH"
     gene            7979..8794
                     /locus_tag="B4U39_RS29260"
     CDS             7979..8794
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267204.1"
                     /locus_tag="B4U39_RS29260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_079406442.1"
                     /transl_table=11
                     /translation="MNLTGVGRRYGRRRPWVLRGITLELPRGALVRIEGGNGGGKSTLL
                     RLLAGIDAPTEGRITGRPARTGYVPERFPAALPLTAASYLTHLGRVHGLPRAEAARRAA
                     DRLERFGAAAYARTPMAELSKGSSQKVAVAQALLAEPGLLVLDEAWTGLDAPARAELDR
                     AVAERTAAGGTVVFVDHDPRRLAGVADAHHRVERGTLVPGSGTQGPRVRIHAVGAPGSP
                     LPPYAGPGAVPAPGGGVLLTVDGERSDAVLHELLTARPPWHIRTVEKLP"
     gene            8791..9435
                     /locus_tag="B4U39_RS29265"
     CDS             8791..9435
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034061.1"
                     /locus_tag="B4U39_RS29265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter"
                     /protein_id="WP_079406443.1"
                     /transl_table=11
                     /translation="MIALLRYQGALLLRSQRWLAPLITYAAFVAAGIRPGDPALDSLGF
                     AAAGLVPVTAWLVRICVTGEPDAARDCTAAAAGPVRVHTAALLTALAGALLAGAAAAAA
                     ALLIGDRAGAGTGGPALAGALAVAACALTGAAVGALCSRPLVRRRGYAVLAGTLGSLAA
                     WLLAPSPAHGAVSALVSAAHSGTAPLPLLPLAGALLLAAGAGTAAALAAAR"
     gene            complement(9436..10425)
                     /locus_tag="B4U39_RS29270"
     CDS             complement(9436..10425)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641835.1"
                     /locus_tag="B4U39_RS29270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_203349291.1"
                     /transl_table=11
                     /translation="MNALSRRLRLALTLTAALLLAGGSLGYVLHARHREQPPGQRADAS
                     FRIEEPGLYFRDAAGGRVARQPDAGPPRVTGGPSCDRFHTAGDHALCLRKLPGLPARTE
                     ALVLDRGLREVRRVTVPGIPNRARVSASGRILSWTTFTTGDSYNTAAFSTRTAILDLRT
                     GYLIKSIEQIPLTLAGARHHDSDVNYWGVSFAADDNRFYATVSTRGATHLVEGDLRAWS
                     AKALRENAECPSLSPDGARVAFKKKVSDDPRAPWRLYVLDLATMRERPLAEPHSVDDQA
                     AWLDDATLAYALPGQDGRASDIWSVPADGTGAPRLLVPGGSSPAHVGQ"
ORIGIN
        1 tcgccggggc gcccgtcgac ctcggcgccg aatccatgct cgcccgccgc cccgaggccg
       61 tggcgctcgc cgaggccgtc ggcctcggcg catccgtgca ggcgcccgcc accgccaccg
      121 cccacctgtg gacccgcggc gcactgcggc cgatgccgcg cggccacgtc atgggcgtcc
      181 ccggcgacct gggaccgctc gccgcctccg gggtgctctc cgccgagggc ctggcccgca
      241 tcgaggccga gcgcacgctg gcgcccaccg agatcggcga ggacgtcgcc gtcggcgagt
      301 acgtcgcggc ccgcctgggc cgcgaggtcg tcgaccggct ggtcgaaccg ctgctcggcg
      361 gtgtctacgc gggcgacgcc taccggatct ccatgcgggc cgccgtgccc cagctcttcg
      421 aggccgcccg cacgcacgcc ctcctcggcg acggagtacg cgaactccag cgccgggccg
      481 aggccgcgcc ccagccgccc ggcccggtct tcgccggaat cgacggcggc atcggacggc
      541 tcccgctcgc cgtggccgag gcctgccggg cggccggcgc gcggctggtc accggcgccc
      601 ccgtccgcga ggtcctccgt acggccgacg gctggcgggt cgtcgccggc gccgaggcca
      661 tcgaggccga cgccgtgatc ctggccaccc cggccggggc cgccgcgcgc ctgctggacg
      721 gcctcgcgcc gaccgcggcc gccgagctgc gcggggtgga gtacgcctcg atggccctga
      781 tcacgatggc cttccgccgc cgcgacctgc cggcggccgt ctccgggggc ggcgccagcg
      841 gattcctcgt accgcccgtc gacggccgca ccatcaaggc ctccaccttc tccagcaaca
      901 agtgggcctg ggcggcagcg gatccggaac tcttcctgct gcgcacctcg gtgggccgct
      961 acgccgacga gagcatcctg ctgcgcgagg acggcgagct cgtcgacgtc tccctcgccg
     1021 acctcggcga ggccgtcggc ctggcggccc ggccggtcgc ctccaccgtc acccgctggg
     1081 acggcggact gccccagtac ccggtcggcc acctcgaccg cgtcgcccgg atccgcggcg
     1141 ccgtcgccgc cctgccgggc ctcgcggtgt gcggcgccgt ctacgacggc gtgggcattc
     1201 cggcctgtat cgcgagcgcc ggcagggccg cggacacggt gatcgccgcg ctgggcgcgc
     1261 gtacggcacc cctggcaccg accactgatc agcgcacggg acaataggca catgactgca
     1321 ccagagaaga ttcccaacgc ggggaagaaa gcgaaggacc tcaatgaggt cattcgctac
     1381 accctgtggt ccgtcttcaa gctgaaggac gttcttcccg aggaccggac cggctacgcc
     1441 gacgaggtcc aggagctctt cgaccagctg ggcgccaagg acatcaccgt ccgcggcacc
     1501 tacgacgtct ccggcctgcg cgccgacgcc gacgtcatga tctggtggca cgccgagacg
     1561 gcggacgagc tgcagaccgc ctacaacctg ttccgccgca ccaagctggg ccgcgcgctg
     1621 gagccggtgt ggtcgaacat ggccctgcac cgcccggccg agttcaacaa gtcgcacatt
     1681 ccggccttcc tggccgacga ggtcgcgcgc gactacgtca gcgtgtaccc cttcgtgcgc
     1741 agttacgact ggtacctgct gcccgacgag gaccgccgtc gtatgctcgc cgaccacggc
     1801 aagatggcgc gcggctaccc cgacgtgcgc gccaacaccg tcgcctcctt ctccctgggc
     1861 gactacgagt ggatgctggc cttcgaggcg gacgagctgt accgcatcgt cgacctgatg
     1921 cgtcacctgc gtgcctccga ggcccgtatg cacgtccgtg aagaggtgcc cttctacacc
     1981 gggcgccgca agtccgtcgc cgacctggtg gccggactcg cctgagacct cccctggggg
     2041 gaaggaccgg aggacgacca caccgtcagg tgagttcctc cggtggatcg ggaggctggt
     2101 ccgaaagacg gcccctgccc ccgaaacgac gcagccggag atcccgcccg gaagttcaga
     2161 cgactggcgg cagcagcgcc ctgcgggcgc ccgctgcccg gtcgtccagc gacaccggtg
     2221 cgggctccgg atgcggcgcg cacgtgacgc gccaccccgg ggtggcgccc gtcagcagat
     2281 accgctccac gtgacggtcc acgcagtcat tgcgtccgcc gaccactccg tgggagccgg
     2341 cccccgcctc cgtcaccagc gaagccccgg cgccgagccg ccgctggagt tcgagcgcgc
     2401 ccgggtacgg ggtcgccccg tcccgctccg ccgccaccac cagggtcgcc ggcagccgct
     2461 gcggcccgtt cccgaccgcc accggccgct gccgctcgcg caccggccag gaggcgcacg
     2521 gcaggttcag gaaggcgttg gcccaggtct cgaagggggc cctgcgggcc agttcggtgt
     2581 ggtcacggtc ccaggtctcc cagtccgagg gccacggggc gtcgttgcac agcaccgccg
     2641 tgtacaccgc cgtcgcgttc tcggcctcgg ccgccgccgc ggggtcggct gcggggccgg
     2701 cctgggcggt cagcggggcc ggatcgccgc gcaggtgggc cgcgagggcc gccgcccggt
     2761 ccggccacac gtcgtcgtag tacgcggcct ggaggtaggc ggcccggagc tcgccggtcc
     2821 ccacgacccc gcccgccggc gtacgggcca ccgcgtcccg gacccgttcg tagctcgcct
     2881 gcacggccgc gggggtggcg ccgaggccgt acgtgtcgtg gtggcgggcc gcccaggcgc
     2941 ggaagtcgta ccagcggcgc tcgaagccgg ccgactggtc gaggttgttg cggtaccaga
     3001 tgcggcgcgg gtcgggatcg accgccgagt cgaggaccat ccggcggacc cggcccgggt
     3061 acagggtggc gtagacggcc ccgaggtagg tgccgtacga ggcgcccatg aagctcagct
     3121 tccgctcgcc gagcgcggcc cgcagcacgt ccagatcgcg ggcgttgtcg agcgtcgtgt
     3181 agtacggcag ggccgcgccg gcccgccgcg agcagccgcg ggcgtacgcc cgggcggccg
     3241 cgaggcgctg cctcttgtac gcgggggagg gctcggccgg tacctgggtg gggcccgcgg
     3301 cccgggtggc ggggtcctgg caggacagcg gggcggagcg gccgacgccg cgcggggcgt
     3361 agccgacgag gtcgtaggcg gcggcgatcg actcccagcc cggcaggtcg gcgacgagcg
     3421 ggaagtgcat gccggaggcc cccgggccgc ccgggttgtg gatcagcgcg ccctggcgga
     3481 cggcgccccg ggcgccggtg gcggcggtcc ggctgacggt gagggagatc tgcgggccgt
     3541 ccgggcgggc gtagtcgagc ggtacgcgca gggtggcgca ccggaccggg ccggagaggc
     3601 cctcgacgtc ggggcagcgg ccgaagtcga ggccgccgcc ccgggccgcg gcgcggcggg
     3661 cggctagggc ggcgccggcg gcctcggccg cctcggcatc cgtcccgccg ggggagccgg
     3721 gcggggtgcc cgccagggcg tgggcgggca ggtgggccgg ggcgcacagg aggagggcga
     3781 gcgccgccac ggcggccgtg cgacccgcga gtcccgtccg catggcggct cctccctctc
     3841 atccgatgaa ccgatgagag gcccgggcgc tgccgttgta aaggatcacc ggcgggtgtc
     3901 ggcgccgatg ctcccgttgc cgcggattcc gcccccggtt gccgtacccg ccggccgtca
     3961 ggagctgctg ctgccgcagc tggagctcga ggagctgccg cagctcgacg aactgccgca
     4021 gctggagctg ctgctcgaac agctggagcc gccgccggag gagccgcagc cgccgcccga
     4081 ggagccgcag cccgtactgc cgccgcagcc cgagccactg ccgcagctcg acccgccacc
     4141 gctgccgccg tcgctcccgg cgcaccagac gatcggcagc acctcggccc cggaccagtg
     4201 cgcggagccg cccgtgccgt gggagcccgt gcgcgactgc gccgcggcca gccgggtccc
     4261 gcgggcggcg ggcaccaact gctggcgcag gtacgggtcc cgcagaccgc gcagcccgaa
     4321 cagcgcggtc tgcacgtgcg gggtccggtc gttcaggtgg gcggagcgga ccgcgcgcag
     4381 cgcacgcgcc ccggccccgg tgacgcggct cttggccccg gccgcacaga ccaggcccac
     4441 gacgatgccg cccaccaggg cgaagatcac cttcatgacg aacggcaccg ccgagccgga
     4501 gtcgacggcg agcgcgacga aggtcagcac catcgacacc gggaacagca gtgcgcacac
     4561 cagggcctgg atcagacccc agcggcggaa gcgccgcagt ccggacccgg gcggggtgat
     4621 cagcccgcgg gcggccagcg cgtccccgat ctcctggacg gccgggtcgc acatggcggt
     4681 gtagcgcacc tggtagagcc atcccgaggg cgctccccgg tgggcctgga ggacggcccg
     4741 ctcggcgggg tcggcggccc gcgccccggg gcgtacctgg acgatgcccg gaccgccggc
     4801 caccagccgg ccgtccccga gcatcgagac gagcgcggcg tccacgacgg tgccggggcc
     4861 gcccaccacg aaggcggcct cggacaggtc gtgcaggccg gcggcgggtc cggcgggcgc
     4921 ggagcgcgct cggcggagcc cggtgatcag cagggcggag gagacgagga cggcgatcca
     4981 gatcgccacg gcgaggaggt tcatgccgct ctccccgcga gcgcccgggc ccagcgcacg
     5041 atccggcgcg gcggcctggc cccggtgcgg tcccgccacc aggcggtgag ccggcgccgg
     5101 gcgacggggt cggccggcag gtcccggatc agcaggtcct cggcgaagtc cagcgcgtcc
     5161 cggcggtacc cggcggtcat cgggcggccc ttggcgtagg agaggaaggc ctcgcggtac
     5221 gggtccgggc cgcccaggat ctccggcagc tcgggagcca gccgggccac cacgtcggcc
     5281 cgcttggcgg ccagagcccg ggcctgcacg cggatccgcc ggcggtcgaa gccctcgggc
     5341 acgggggtcc cggccaccag cgccgacagc agcgcggcct gcccgagcgc cacccgggtc
     5401 cgggccgact cctcgaccgg acgcggctcg tcggcggcat cccgctctgc ggcggcgtcc
     5461 ggctctgcat ccgggcgcgg ctcggcggcc gccgggccgg acccggcgag ggcgcggtcg
     5521 gcgacggcgg ccggccccgc cgtcaccacc gcgcggatcg cggccagttc gcccgcgagc
     5581 tccgcctccg cggggaagtc gtcgtcgcgc tccagcagga cgcccggcgg gtccacccgg
     5641 gagcgcagcg cggccagcag gtcgaggacg accggcggga ccgggtgcgc gtgggtgtcg
     5701 tgccagacgc cgccccgttc gatcccgccc gccacgtgca cgtacgcgag tgcctccagc
     5761 gggatcgcgt ccagcactgc ggccgggtcc tcgccccggt tgacccggtt ggtgtgcagg
     5821 ttggccacgt cgatcagcag ccgcacaccg gtccgctcga ccagttccgt gaggaactgc
     5881 gcttccgtca gctcctcgcc cggccaggag aacagcgcgg cgatgttctc cagggccagc
     5941 ggcacgggca gggagtcctg ggcgatccgc acgttctcgc acagcacgtc cagcgcgtcg
     6001 cgggtgcgcg gcaccggcag caggtgcccg gcctccagca ccggcgagga ggtccgtacg
     6061 aaggcgatgt gctcagtgac gagcggcgcc cccagcgcca ccgcccgttc gccgagcgcc
     6121 gcgagcttcg ccggatcggg gcggtccgcc ccgccgaggc cgagcgagac cccgtgcggc
     6181 accacgcgcg tgccccgctc gcgcagccgc agcagggact cgggcaggtg gcccgggcat
     6241 acgttctcgg ccaccacctc gacccagtcc aggccgggaa gccgttcgac ggcgtccgcg
     6301 atctccggcc gccagccgat gcccaccccc aggtgtgcca tgggtttcat gtcccctcgc
     6361 cccctctcgt cgcaccgtgt gccggtgtca tggccccgac ggagtcgcgc caaccgcaga
     6421 gggggacgtt cagaggaaca tttgaggttc ccgccgaccg cggtacctgt cagagccggt
     6481 ccgtcacagc cgggcccgca gcgccggatg gtcggcgacc accgtcgcgc tgcccggcgc
     6541 gatctcggtg aacccggcgt cccggaccac cggcagtccg ctcccgccca gctccgccca
     6601 ccgctcgcgc ggggccgtcc gtaccgccag ccggaaaccg gactcctgcc aggcggcccg
     6661 ctccgccccc gtcagctccc accacgccag ctgggccgcg tgcccggcct gggccatggc
     6721 cttgccggcc gacatgtcga ggcccgggtt gagccacagc accggcaggc ccggttccgt
     6781 gacggcggcg accggctccg ggtcgtccag atcggtgccc gacacctgga gcttggccag
     6841 ttccttgggc cagccgtcca ggggcaccgg cgggaagacc cgtacctgcg cgccgtcccc
     6901 gtcgaccgtg acgcccggca gggtctccgc cttgcgccac tccgccccgc gggcgcgccg
     6961 gaccaccttg cggatccgtg cgtcctgcca gtcgcggacc gccccggccc actcgccgtc
     7021 gccctgcgcc cgctcgtcgg tgagcaggac cagcaccgcg cgggccgccg tctccagggc
     7081 gtcggtacgg gcgggcggct cggccttctc gatccgcacc accagcggca gcacgaactg
     7141 cggggcctcg tcgcgccgta cgtgttcctg ccggaagggg ctgtcctccc cgaccgcggg
     7201 gacgtcggag atgtcgttgg tgtgctggct gctcatcccg ccaaggatgc cagcctccac
     7261 cttcaggatg atgccccggg tgggcggccc gggtgaggat cgcagccatg aagagtgacc
     7321 ttttcgcgtc cgagaacctg gcccggacgg ccgtcgcccc cggcatgagc ctccagaacg
     7381 ccaagtcggt gaagtacaac gtcaacggtg aaatgctggc ccgccagggc tcgatggtcg
     7441 ccttccgcgg caacctccag ttcgagcgca agggacaggg cataggcggc atgctcaagc
     7501 gcgccgtgac gggcgagggc ctcgcgctca tgtccgtacg cgggcagggc gaggcctggt
     7561 tcgcccacca ggccggcaac tgcttcatca tcgacttcga gccgggtgac gccctcacca
     7621 tcaacggccg caacgtgctc tgcttcgacc ctacgctgtc ctacgagatc aagatggtga
     7681 agggcgccgg catgaccgga ggcggtctct tcaacagcct cttcaccggc accggcaagc
     7741 tcgccgtcgt ctgcgacggc cacccgatca tcatcccggt cacccctcag gcgccggtcc
     7801 acgtggacac ggacgcggtg gtcggctgga gcgcccagct ggagaccggc ctgcaccgct
     7861 cccagtcggt cggctcgatg atccgcggcg gctccggcga ggcggtccag ctgaagctga
     7921 gcggcgaggg cttcgtcatc gtccgcccga gcgaggtcac cgagacggcg gccgggcact
     7981 gaacctgacc ggtgtgggcc gccgctacgg acggcgccgg ccatgggtgc tgcgcgggat
     8041 tacgctggag ctcccgcgcg gcgccctcgt acggatcgag ggcggcaacg gcggcggcaa
     8101 gtccaccctg ctgcggctcc tcgccgggat cgacgccccg accgaggggc gcatcacggg
     8161 ccgcccggcc cgcaccggtt acgtacccga acgcttcccg gcggcgctgc cgctcacggc
     8221 agcctcctac ctgacccacc tgggccgggt gcacggactg ccgcgcgccg aggcggcccg
     8281 ccgggcggcc gaccggctgg agcggttcgg cgccgccgcg tacgcccgaa ccccgatggc
     8341 cgaactgtcc aaggggagca gccagaaggt ggccgtcgcc caggccctgc tcgccgaacc
     8401 ggggctgctc gtcctcgacg aggcgtggac cggcctggac gcccccgccc gggccgagct
     8461 ggaccgcgcc gtcgccgagc gcaccgcggc cggaggcacc gtcgtgttcg tcgaccacga
     8521 tccgcgacgg ctcgcgggcg tggccgacgc ccaccaccgg gtcgagcgcg ggaccctcgt
     8581 ccccgggtcc gggacgcagg gaccccgcgt gcggatccac gccgtcggag cgcccggcag
     8641 cccgctgccc ccgtatgcgg gcccgggcgc ggtgcccgcc ccgggtgggg gagtactgct
     8701 caccgtcgac ggcgaacgct ccgacgcggt cctgcacgaa ctgctcacgg cccggccgcc
     8761 ctggcacatc cgcaccgtgg agaaactccc gtgatcgccc tgctccgtta ccagggcgcc
     8821 ctgctcctgc gctcccagcg atggctcgcg cccctgatca cgtacgccgc cttcgtggcc
     8881 gccgggatcc gcccgggcga cccggcgctg gactccctcg ggttcgcggc cgccggcctg
     8941 gtcccggtca ccgcctggct ggtccggatc tgcgtgaccg gcgaacccga cgcggcccgc
     9001 gactgcacgg ccgcggcggc cggtcccgta cgggtccaca cggcggccct gctcaccgcc
     9061 ctggccgggg cactgctcgc cggagccgcg gcggccgccg cggccctgct catcggcgac
     9121 cgggccgggg ccggcacggg cgggcccgcc ctcgccggag ccctcgcggt ggcggcctgc
     9181 gccctgaccg gcgcggccgt gggcgccctg tgcagccggc cgctggtccg gcgccggggg
     9241 tacgccgtcc tggccgggac cctcggctcc ctggcggcct ggctgctcgc gccctccccg
     9301 gcccacggcg cggtctccgc gctggtctcg gccgcgcact cggggacggc gccgctgccg
     9361 ctcctgcccc tggccggtgc gctgctgctg gccgccgggg cggggacggc ggccgccctg
     9421 gcggccgcgc gctagctact ggccgacgtg ggcgggggag gagccgcccg ggacgaggag
     9481 acggggtgcg cccgtgccgt ccgccgggac cgaccagatg tcgctcgcgc ggccgtcctg
     9541 cccgggcagg gcgtaggcga gggtcgcgtc gtcgagccag gcggcctggt cgtcgacgct
     9601 gtggggttcg gccagtggcc gctcgcgcat cgtcgccaga tccaggacgt acagccgcca
     9661 gggcgcccga gggtcgtcgg agaccttctt cttgaaggcg acgcgggcgc cgtccgggga
     9721 cagcgagggg cattcggcgt tctcccgcag cgccttcgcc gaccaggcgc gcaggtcgcc
     9781 ctcgacgagg tgggtggcgc cccgagtgga gacggtggcg tagaagcggt tgtcgtcggc
     9841 ggcgaagctc acgccccagt agttgacgtc ggagtcgtgg tggcgggccc cggcgagggt
     9901 cagcgggatc tgctcgatcg acttgatcag gtagccggtg cgcaggtcca ggatcgccgt
     9961 ccgggtggag aacgcggcgg tgttgtagga gtcgccggtc gtgaaggtgg tccaggacag
    10021 gatccggccc gaggccgaga cgcgggcccg gttcgggatg ccgggaacgg tcaccctgcg
    10081 gacctcccgc agcccccggt cgagtacgag cgcctcggta cgggccggga gcccgggcag
    10141 cttgcgcagg cacagggcgt ggtccccggc ggtgtggaag cggtcgcagg acgggccgcc
    10201 ggtcacgcgg ggcgggcccg cgtcgggttg gcgcgcgacc cgcccgccgg ccgcgtcccg
    10261 gaagtagagg ccaggctcct cgatgcggaa ggaggcgtcg gcccgctgcc cgggcggctg
    10321 ttcgcggtgt cgcgcgtgga gtacgtagcc cagcgagccg cccgccagca gcagggccgc
    10381 ggtgagggtg agggcgaggc ggagccgacg ggacagcgcg ttcacggggc ctccggtgcg
    10441 gttggggaag gggcgggcgc gtcaacgggc atgaggcgtg aggcggccgc gagcgccccg
    10501 gccagggcga gggccgcggc ccagagggcg ggccgtgcgc cccagagggt ccaggccgcc
    10561 ccgaatccgg cggcgcccag cagccgggcc aacgcctggc cggtctgcag cacggcgagc
    10621 ccgcccgccc ggccggccgc gggcagcgcg ggcgcggcga gcgccatcag gaccccgtcg
    10681 gtggcggcgt agaagacgcc gagcagggcg agcaccccca cgacggtcgg cggggagacg
    10741 ggggcgagca gcagcacgta ggcgccgagc agggcggcgt gcccggtcag gaaggccgtc
    10801 cggcggccgg tgcggtcggc gatccggccc gcggggacgg cgagcagcag gtagacggcg
    10861 gcggccccca gcggaagcag cgtgaacagg gcgggcggca ggtcgagtcc gcgcaggagc
    10921 agcaggtaga ggaaggagtc gccgatggtg gtggcgccga gtacggccga ggcgtacagc
    10981 acgcggcgga aggccggatc gcgcagggcc gagggccgcg gtggggcggc ccggggcgcg
    11041 gtgggcgtgg ggggtgcggt gggtgcggtg gccggacccg gcgcggccgg gacgtacacc
    11101 acgagcagga gcacgccgag caggccggtg cagaaactga cggcgaacac cgcgtcatag
    11161 gcatcggcgg tggcccacag cacggcgaac gcggcgagcg gcccgagcag ggctcccgtg
    11221 gtgtccatgg cgcggtgcac gccgaaggac cggccgaggg tctccggggg cccgctgagc
    11281 gagatcaggg cgtcgcgcgg tgcggtacgg atgcccttgc cgagccggtc ggcggcgagt
    11341 gccgcggcga
//
