LOCUS       NZ_CP020039            13009 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. 3211 isolate 3 chromosome, complete genome.
ACCESSION   NZ_CP020039
VERSION     NZ_CP020039.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 3211
  ORGANISM  Streptomyces sp. 3211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Complete genomes of three Streptomyces isolated from disease
            suppressive soils
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2017) Biotechnology Institute, University of
            Minnesota, 1479 Gortner Ave, Saint Paul, MN 55108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JAN-2017
            Assembly Method                   :: PacBio v. Jan-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:18:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,254
            CDSs (total)                      :: 8,154
            Genes (coding)                    :: 7,815
            CDSs (with protein)               :: 7,815
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 206 of 340
            Pseudo Genes (incomplete)         :: 257 of 340
            Pseudo Genes (internal stop)      :: 68 of 340
            Pseudo Genes (multiple problems)  :: 153 of 340
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:04:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6280283
            Orig. end                         :: 6293292
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Michael Smanski.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..13009
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="21"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..13009
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13009
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8009](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8009
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(794..808)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.12"
                     /tool="antismash"
     gene            complement(931..2325)
                     /locus_tag="B4U39_RS28980"
     CDS             complement(931..2325)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1172: methionine aminopeptidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965659.1"
                     /locus_tag="B4U39_RS28980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminopeptidase P family protein"
                     /protein_id="WP_079406417.1"
                     /transl_table=11
                     /translation="MTDAPADASAPTPARLNTGSHDRPVSAELSRYMAGHWAASPLPDS
                     ARVPGHAVTPARRARLSARFPGERLIVPAGELKVRTNDCDHRFRPHSAYAWLTGLTGED
                     QAGHVLVMEPSGPHAHEAVLYLRPRSPRADGDGEFYRDRRYGEFWVGRRPDLAEAERLT
                     GIRCAHLDGLGSPAGRNAATDPELASALSELRLVKDVWEVEQLQLAVDHTTAGFEDVVR
                     ALPRALAHPRGERWIEGVFGLRARAEGNGTGYETIAASGAHACTLHWIRNDGRLNGAEL
                     LLLDAGVETDTLYTADITRTLPLSGRFSPVQRQVYELVLAAQEAGIAALRPGASFGDFH
                     RAGMRVIAEGLAEWGVLKDAEGDLHRRYTLCSSGHMLGLDVHDCAKARAETYLDGVLEE
                     GQVLTVEPGLYLQPDDETLPAELRGIGVRIEDDLVITADGARLMSGALPRTVAGIEEWM
                     GRLLER"
     misc_feature    2372..2388
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.29"
                     /tool="antismash"
     misc_feature    2376..2386
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            2499..4883
                     /locus_tag="B4U39_RS28985"
     CDS             2499..4883
                     /EC_number="3.4.24.3"
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267147.1"
                     /locus_tag="B4U39_RS28985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="collagenase"
                     /protein_id="WP_030386125.1"
                     /transl_table=11
                     /translation="MLKHRAVRSSLLASAVAVTLLATAGQAATQAAASAAPATSTGAQA
                     APAAPAPGPTGNPFDEVDRLANPKDNTPAPAPAPGGQNITGQVPGAATAAAPADDLQKR
                     GKAPKAAPTAKSVAAGVPCTLDGITGLTPEQFADFLGDQAVLADGCLRGLIWTWDARLT
                     PVMSDAHVQAVSRRISSLAAAHDGRNSSHLEEMFTYLHAVAYHDHSRSEIDVTDAPTVD
                     AMRRAIADFAGAARTFDVTPSNARTLREALYAGSAPGLRQHQLGLIKKVLATMDPAHPA
                     THLDAGWAGAALAALSVSYLGVYPGNQDAAFHSAVAADPAYRDTFKAFSTYTHLKGTGN
                     AWVVRDALSEYARFGQVEGLRDRVVADLGAMLGPVRAAFGDGSEPWAKIVSWLNVYEAC
                     KQYGVCKEDIEKQLFPYTYTYDNGAIKVRTALDRATVDQLYYASKQVKAQYHRVLGTDQ
                     PLAGDPNTTLNIVLYASRADYVNYHPILTGYDTNNGGIYIENGATFYTYQRRVPQDSSL
                     TLEELFRHEYTHYLNGRFAVPGFFGEGEWYEGDRTTAMDEGTAEFFDGATRDNGIAVRK
                     SLVKSIISDTAGGGPRMSVEQLLNATYDGDGFRFYSYAGTFFEFLWTEKPTLLREMYTH
                     LRADDVVAYDAWRHRMGSDAYLQRDYDRFLDAQIAKVDQLFVPNTSFTPNDRLRDSALA
                     SVKSSFAAATYNTPDCVENGDPGKRRFTCTGRITANLKNWRNNDQVFKDMSETVDYFIL
                     DRAGAASNNLADMNCSFGAVEIWTNKVAGTSSYSCEGPLRS"
     gene            5001..6176
                     /locus_tag="B4U39_RS28990"
     CDS             5001..6176
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891271.1"
                     /locus_tag="B4U39_RS28990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_079406418.1"
                     /sec_met_domain="DAO (E-value: 5.5e-67, bitscore: 225.6,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKRHSLDVVIIGAGVVGAACAYYAARAGLSVAVVDRGPVAGGTT
                     GAGEGNLLVSDKEAGPELDLALLSTRLWTELAAALPPEVEYEPKGGLVVAPDETTFKAL
                     RTFAEGQRAAGVDAVEVAADALPELEPHLAPGSAGGFHYPQDAQVQPAQAAARLLAASG
                     ARLHLGEEVTQVLLDRGAVRGVRTPRRELLAPAVVNAAGTWGGHIASLAGVNLPVLPRR
                     GFVLVTEPLPRVVRHKVYAADYIADVASGSAALQSSAVVEGTPSGPVLIGATRERVGFD
                     RTLSTEALRRLASQAAALFPVLADVRVMRTYHGFRPYLPDHLPAIGPDPRRPGLLHACG
                     HEGAGIGLAPATGLLVAAALTGDEPPLPAHPFRPDRFAAGPAAADTPEEQH"
     misc_feature    5020..5034
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.01"
                     /tool="antismash"
     gene            6176..6490
                     /locus_tag="B4U39_RS28995"
     CDS             6176..6490
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543028.1"
                     /locus_tag="B4U39_RS28995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_079406419.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.6e-25, bitscore: 87.8,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRSPRSLVGGTPVAAYEITFDGRELPALPGQSIAAALWGAGVLA
                     WRTTRDGGAPRGAFCGIGSCFDCLVTVNGRPNQRACLVPARPGDTVTTQEGTGRADLAV
                     "
     gene            6555..8009
                     /locus_tag="B4U39_RS29000"
     CDS             6555..8009
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850112.1"
                     /locus_tag="B4U39_RS29000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_079408079.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5.9e-22, bitscore:
                     77.0, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 5.1e-12, bitscore:
                     44.8, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPAPTDGPAAARTDLAIVGAGPAGLAAAVTAAGLGLRVALLDAGD
                     RPGGQFYRHPAPGLGAARPEALHHGWADFATREAALRAHESAGRITYLPLHHVWAVVPA
                     DAGEAGEAAGAGRGDAAWILHAVAGHAPEERAAAVTARAVLIATGAYERQLPFPGWTLP
                     GVVGAGGAQAMLKSGLVLPGKRVVVAGSGPLLLAVAGSLAAAGATVPAVVEAAAYTAYA
                     GRVPALLRNPGKLVEAAGYGGALARHHVRLLTRHAVTEAHGTDRVEAVTVARLDRDWRP
                     LPGTGRRIPCDALAVGHGLVPQLELATGLGCATVRSADGAVALALDAEQRTSVPGIWSA
                     GETGGIGGAQLAMIEGELAAHSVAGALPGARLTRRRARLRAFADAMAAAHRPGAGWTGW
                     LRDDAVVCRCEEVPAGRIREAAEDLGARDARTVKLLTRAGMGWCQGRMCGPAVAALAGE
                     EAAADRRPLSCPVPLRQLAELPPAGN"
     misc_feature    complement(8045..8061)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
     misc_feature    8051..8061
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            8085..8999
                     /locus_tag="B4U39_RS29005"
     CDS             8085..8999
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267151.1"
                     /locus_tag="B4U39_RS29005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_079406420.1"
                     /transl_table=11
                     /translation="MTHAHTPAPRTRPWHGIMVATALPLREDLTVDYDAYAEHVAWLIA
                     NGCDGVVPNGSLGEYQTLTDEERARVVRTAVEAAGDGARVMPGVAAYGSAEARRWAEEA
                     AEAGAGSVLLLPPNAFRADEEAVRAHYAEVARAGLPVVAYNNPIDTKVDLTPALLARLH
                     ADGSIVAVKEFSGDVRRAYEIAELAPGLDLLIGADDVLLELALAGAVGWIAGYPNALPN
                     ACATLYRAAVAGDLDTALPLYKSLHSLLRWDSKTEFVQAIKLSMDLAGRPGGATRPPRF
                     PLTGETEAAVRAATEKALAEGLN"
     gene            9019..10023
                     /locus_tag="B4U39_RS29010"
     CDS             9019..10023
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="B4U39_RS29010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_030386120.1"
                     /transl_table=11
                     /translation="MRTRHVYHAVDSHTEGMPTRVITGGVGVVPGATMAEKRLHFIEHM
                     DHVRTLLMYEPRGHSAMSGAILQPPTRPDADYGVLYIEVSGLLPMCGHGTIGVATVLVE
                     TGMVPVVEPVTTVRLDTPAGLVSVDVRVEDGAATAVTLTNVPSFCVGLDLKTDVPGFGT
                     ITYDLAYGGNFYAFVELDALGLPFDRDRGDDLIAAGLAVMDAINAGPDRPVHPENPSIA
                     GVKHVYLAAPGSDAHRSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGLLELGADFANE
                     SFIGTEFTGRLVGETTVGGLPAVVPTVTGRAWITGTAQYFLDPTDPFPGGFLL"
     gene            10020..11039
                     /locus_tag="B4U39_RS29015"
     CDS             10020..11039
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018844380.1"
                     /locus_tag="B4U39_RS29015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_237296847.1"
                     /transl_table=11
                     /translation="MITVRTVDYHTAGEPFRIVDSAATGMPPVPGDTVAERCATVIGPG
                     GSGTAPRRGALDDVRRLLVQEPRGHAGMYGGFVVPADDDGAHFGVLFWHKDGYSTACGH
                     GTMALGAWAVDTGRVAAPDDGDAPVRIDVPSGRVTATVHRAAGRTTGVTFRNVPALVGA
                     RKVPVATSFGLAEVDIAHAGACYAAVRARDLGLDVSRAALPSLVRAGQEIRAALATHPA
                     TWHPGGPLLSGVYGVILHEELPDTPFGPHQRNVTVFADGQIDRSPCGSGTSARLALLAE
                     DGRLGPGEDLLHESVTGTVFTGRMLEGGVTEVTGTAHRTGEHAFVLDPHDALGTGFLL"
     gene            11036..11977
                     /locus_tag="B4U39_RS29020"
     CDS             11036..11977
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038315.1"
                     /locus_tag="B4U39_RS29020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="WP_237296848.1"
                     /transl_table=11
                     /translation="MTDILRLDAGRMAGLLTPAGAADALAAALRGGLDPESCPPRSAIA
                     VPGGGELLLMPAASGAYAGVKIAGVAPGNPDRGLPRITGSYLLLDGPTLRPLALLDGAA
                     LTTLRTPAVSALALRHLAPAGRPLRMVLFGSGPQAYGHLEAVLAVRELAEVVVVARHPG
                     RAAELAAHARTLGPAARTGTAGEVAGADLVICCTTAREPLFDGRLVGPGATVVAVGSHE
                     PTARETDTALVRRAAVYVESRAAALREAGDLLVPEAEGAIGPGHITATLADLVGGRIPA
                     GGGQSCPQLFKSVGMAWEDLAVAVALVEAAGV"
     misc_feature    complement(11988..11998)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            12049..12762
                     /locus_tag="B4U39_RS29025"
     CDS             12049..12762
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237887.1"
                     /locus_tag="B4U39_RS29025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_030386117.1"
                     /transl_table=11
                     /translation="MGDLKQHSLIKAQERLRDQVGHALRAALIAGELRPGSVYSAPGLA
                     AELGVSATPVREAMLDLAREGLVEPVRNKGFRITEVSERDLDQFTELRTMIEVPTIGRI
                     TKIATAEQLEALRPIAEEIVSSAREHNLIGYLEADRRFHLSLLALAGNDRLVETVGDLR
                     KRSRLYGLTGLDEAGKLVSSAEEHIELLDLMIGGDAEAAQECMLRHLGHVRSLWAQGRD
                     EPVGRVPGGLGSGLQ"
     misc_feature    12929..12945
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 18.75"
                     /tool="antismash"
     misc_feature    12933..12943
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
ORIGIN
        1 gccgcggccg tggcgatggc cttcctgctc gtcttcggcc tcgtgggggg atggatcctc
       61 gccggccgga tgctcgcacc gctcgcacgg atcacggagg cggcccgcaa ggccggcgcc
      121 ggctcgctgt cccaccggat ccgcatgaag ggccgccggg acgagttccg tgaactctcc
      181 gacgccttcg actcgatgct ccagcaactg gagtcccacg tcgccgagca gcagcggttc
      241 gccgcgaacg cctcccacga actgcgcacc ccgctggcca tctcgcgcac gctcctcgac
      301 gtcgccggca aggaccccac gcgggaccgg ggcgagctca tcgcacgcct gcaggccgtc
      361 aacacgcggg cgatcgacct caccgaggcc ctgctgctgc tcagccgcgg cgaccgcgca
      421 gacttccgcc ccgagagcgt cgacctctcg ctcgtcgccg aagaagtcgc cgaaacgctg
      481 ctccccctcg ccgagcagcg cgggatcacg ctcgacgtca ccggcgggac ggcccggacc
      541 agcggctccg cggagctcct gctgcggatg gtgacgaacc tcgtccagaa cgccgtcgtc
      601 cacaacctcc ccgccggcgg cacggtctcg gtccacaccg aggcgtccgc ggacacgagc
      661 gtgctgcggg tcgagaacac gggccgcccg ctcccaccgg acctggtggc gaccctcacc
      721 gagcccttcc ggcgcggaac ggaacgcgta cgcaccgacg agcacgccgg cgtcggcctc
      781 gggctggcca tcgtggacag catcgtccgc gcccacgacg ggaccctcga cctcgccgcc
      841 cgccccgccg gcggcctcct cgtcacggtc cggctgcccg gcaccccgta ggccggggtg
      901 ccgggccgat cacgccggcg ggggaggggc tcagcgctcc agcagccggc ccatccactc
      961 ctcgatgccg gcgaccgtgc gcggcagcgc acccgacatc aggcgggcgc cgtccgccgt
     1021 gatgaccagg tcgtcctcga tgcggacgcc gatgccgcgc agctccgcgg ggagcgtctc
     1081 gtcgtcgggc tggaggtaga ggcccggttc caccgtcagg acctggccct cctccaggac
     1141 gccgtccagg taggtctcgg cacgcgcctt cgcgcagtcg tgcacgtcca gtcccagcat
     1201 gtggccgctg ctgcacagcg tgtaccgccg gtgcaggtcg ccctccgcgt ccttcagcac
     1261 gccccactcg gccaggccct cggcgatcac ccgcatgccc gcccggtgga agtccccgaa
     1321 gctcgccccc gggcggagcg ccgcgatgcc ggcctcctgg gcggccagga ccagctcgta
     1381 cacctggcgc tggacggggg agaagcggcc cgacagcggc agggtgcggg tgatgtccgc
     1441 cgtatagagg gtgtccgtct ccacccccgc gtccaggagc agcagttccg ccccgttcag
     1501 ccggccgtcg ttgcggatcc agtgcagggt gcaggcgtgg gcgccggacg cggcgatcgt
     1561 ctcgtacccg gtgccgttgc cctcggcccg ggcgcgcagg ccgaagaccc cctcgatcca
     1621 ccgttccccg cgcggatgcg ccagggcgcg cggcagggcc cgtacgacgt cctcgaagcc
     1681 cgccgtcgtg tggtcgacgg ccagctggag ctgttcgacc tcccacacgt ccttcaccag
     1741 acgcagttcc gagagcgcgg aggcgagttc ggggtcggtg gccgcgttgc ggccggccgg
     1801 tgagccgagt ccgtccaggt gggcgcagcg gatgccggtg aggcgttcgg cctccgccag
     1861 gtccgggcgg cggcccaccc agaactcccc gtaccggcgg tcccggtaga actccccgtc
     1921 cccgtccgcg cgcggcgagc gcggccgcag gtacagcacg gcctcgtggg cgtgcgggcc
     1981 cgagggctcc atgacgagga cgtggcccgc ctggtcctcg ccggtgaggc cggtcagcca
     2041 ggcgtacgcg ctgtgcgggc ggaaccggtg gtcgcagtcg ttggtgcgga ccttcagctc
     2101 accggccggg acgatcagcc gctccccggg gaagcgggcc gagagccgcg cccggcgggc
     2161 cggggtgacg gcgtggccgg ggacgcgggc ggagtcgggc agcgggctcg cggcccaatg
     2221 ccccgccatg taccgggaca actcggcgga tacagggcgg tcgtgactgc ccgtgttgag
     2281 tcgggcgggg gtgggggcgg aggcgtcggc gggggcgtcg gtcacgagga ctccctcaag
     2341 aaagagcgaa ctggggggcg cacaggtctt gtcagtgcaa tttcacatta ctatgttaca
     2401 gctcacaccg cggcgcatta acccccgtca aacgccgtgt gacgcagggc agttcacgca
     2461 ccacccccca cctcacctct ccccatcagg agttctcggt gctcaagcac agagctgtgc
     2521 gctcttccct tcttgcctcc gccgtggccg tgacccttct cgccaccgcg ggacaggccg
     2581 ccacgcaggc cgccgcatcc gcggccccgg ccacctccac cggcgcacag gccgccccgg
     2641 ccgcccccgc ccccggcccc acggggaacc ccttcgacga ggtcgaccgc ctggccaacc
     2701 cgaaggacaa caccccggcg cccgccccgg ccccgggcgg gcagaacatc accgggcagg
     2761 tccccggcgc ggcgacggcc gccgcacccg ccgacgacct gcaaaagcgc ggcaaggcgc
     2821 ccaaggcggc ccccaccgcg aagagcgtcg ccgcgggcgt cccctgcacc ctcgacggga
     2881 tcaccggcct caccccggag cagttcgccg acttcctcgg cgaccaggcc gtcctcgccg
     2941 acggctgtct gcgcgggctc atctggacct gggacgcccg cctgaccccg gtcatgtccg
     3001 acgcgcacgt ccaggccgtc tcccgccgga tatccagcct ggccgccgcc cacgacggcc
     3061 gtaactcctc ccacctggag gagatgttca cgtacctgca cgcggtcgcc taccacgacc
     3121 actcgcgctc cgagatcgac gtcaccgacg cccccaccgt cgatgccatg cgccgggcca
     3181 tcgccgactt cgcgggcgcc gcccgcacct tcgacgtgac ccccagcaac gcccgcaccc
     3241 tgcgcgaggc cctctacgcc ggcagcgcgc ccggcctgcg ccagcaccag ctgggcctca
     3301 tcaagaaggt cctggccacc atggacccgg cccacccggc cacccatctc gacgccggct
     3361 gggcgggcgc cgccctcgcc gccctgtcgg tctcctacct gggcgtctac ccgggcaacc
     3421 aggacgcggc cttccactcc gccgtggccg ccgaccccgc ctaccgcgac accttcaagg
     3481 ccttctccac ctacacgcac ctcaagggca ccggcaacgc ctgggtggtg cgcgacgcgc
     3541 tgagcgagta cgcccgcttc ggccaggtcg agggcctgcg ggaccgggtc gtcgccgacc
     3601 tcggcgcgat gctcggcccc gtccgggccg ccttcggcga cggcagcgag ccctgggcca
     3661 agatcgtctc ctggctcaac gtctacgagg cctgcaagca gtacggggtg tgcaaggagg
     3721 acatcgagaa gcagctcttc ccctacacct acacctacga caacggcgcc atcaaggtcc
     3781 gcaccgccct ggaccgcgcc acggtcgacc agctgtacta cgcgagcaag caggtgaagg
     3841 cccagtacca ccgcgtgctc ggcaccgacc agccgctcgc gggcgacccc aacaccacgc
     3901 tgaacatcgt gctctacgcc tcccgcgccg actacgtgaa ctaccacccg atcctgaccg
     3961 gctacgacac caacaacggc ggcatctaca tcgagaacgg cgccaccttc tacacctacc
     4021 agcgccgcgt cccgcaggac tcctccctca ccctcgagga gctcttccgc cacgagtaca
     4081 cgcactacct caacggccgt ttcgcggtgc ccggcttctt cggcgagggc gagtggtacg
     4141 agggcgaccg gacgaccgcc atggacgagg gcacggccga gttcttcgac ggtgccaccc
     4201 gcgacaacgg catcgccgtg cgcaagtccc tggtcaagag catcatcagc gacacggccg
     4261 gcggcggccc gcgcatgagc gtcgagcagc tgctgaacgc cacctacgac ggcgacggct
     4321 tccgcttcta cagctacgcg ggcaccttct tcgagttcct gtggaccgag aagcccaccc
     4381 tgctgcgcga gatgtacacg cacctgcggg ccgacgacgt ggtcgcctac gacgcctggc
     4441 ggcaccggat gggctccgac gcctacctcc agcgcgacta cgaccgcttc ctggacgcgc
     4501 agatcgccaa ggtcgaccag ctcttcgtgc cgaacacctc cttcaccccg aacgaccggc
     4561 tgcgcgactc ggcgctcgcc tcggtgaagt cgtccttcgc cgcggccacg tacaacaccc
     4621 cggactgcgt ggagaacggc gaccccggca agcgccgctt cacctgtacc ggccggatca
     4681 ccgcgaacct gaagaactgg cgcaacaacg accaggtctt caaggacatg tcggagacgg
     4741 tcgactactt catcctcgac cgggcgggcg cggcctccaa caacctcgcc gacatgaact
     4801 gctccttcgg ggcggtggag atctggacca acaaggtcgc gggcacctcc agttacagct
     4861 gtgagggtcc gctgcgcagc tgaccgccgc cggttcgccg ccgaccccgg cccggctccg
     4921 gaccggtccc gcatccgagg ggggtgcggg accggtccgg gtgtctgggc aggccctaaa
     4981 gaatgtgaca tattactgcc gtgctcaaga gacactccct ggacgtcgtg atcatcggcg
     5041 ccggcgtcgt cggggcggcc tgcgcgtact acgcggcccg cgccggactc tccgtggccg
     5101 tggtcgaccg gggccccgtc gcgggcggca ccaccggcgc cggcgaaggc aacctgctcg
     5161 tctccgacaa ggaggccggc cccgaactcg acctcgccct gctgtccacc cggctgtgga
     5221 ccgaactcgc cgccgccctc ccgccggagg tcgagtacga gcccaagggc gggctcgtcg
     5281 tcgcccccga cgagaccacc ttcaaggccc tgcggacctt cgcggagggc cagcgcgcgg
     5341 ccggcgtcga cgccgtcgaa gtggcggccg acgccctgcc cgaactggaa ccgcacctgg
     5401 ccccgggctc ggcgggcggc ttccactacc cgcaggacgc ccaggtccag cccgcgcagg
     5461 ccgcggcccg gctgctggcc gcctcgggcg cccgcctgca cctcggcgag gaagtgacgc
     5521 aggtcctgct ggaccggggc gccgtacgcg gggtgcgcac gccccgccgt gaactcctgg
     5581 cccccgccgt ggtgaacgcg gccggcacct ggggcggcca cattgcctca ctggccgggg
     5641 tcaacctgcc cgtcctcccg cgccgcggct tcgtcctggt gaccgaaccc ctgccgaggg
     5701 tggtccggca caaggtctac gccgccgact acatcgcgga cgtggccagc ggctccgccg
     5761 ccctgcagtc ctcggcggtc gtcgagggca ccccgtcggg cccggtcctg atcggcgcca
     5821 cccgcgaacg cgtcggcttc gaccgcaccc tctccacgga ggccctgcgc cggctggcct
     5881 cccaggcggc ggcgctcttc cccgtcctgg cggacgtccg ggtgatgcgc acctaccacg
     5941 gcttccgccc ctacctgccc gaccacctcc cggcgatcgg cccggacccg cggcgccccg
     6001 gactgctgca cgcctgcggc cacgagggcg ccggcatcgg cctcgccccc gccaccggac
     6061 tgctggtcgc cgccgccctg acgggggacg aaccgccgct ccccgcccac ccgttccgcc
     6121 cggaccgctt cgccgccggc ccggccgccg ccgacacccc cgaggagcag cactgatgcg
     6181 ccgcagcccc cgctcgctgg tcggcggtac gccggtggcc gcgtacgaga tcaccttcga
     6241 cgggcgggaa ctgcccgccc tgcccggtca gagcatcgcc gccgcgctct ggggcgcggg
     6301 cgtcctcgcc tggcggacca cccgcgacgg cggcgccccg cgcggggcgt tctgcgggat
     6361 cggcagctgc ttcgactgcc tcgtcaccgt caacggccgc ccgaaccagc gggcctgcct
     6421 ggtccccgcc cggcccgggg acaccgtcac cacccaggag ggaaccggcc gtgccgacct
     6481 cgccgtctga acccgccggc tgcccgggcc gcgcgggccg cgcgcatccc gagagcccgg
     6541 caggccctgc cgccgtgccc gcgcccacgg acgggcccgc ggcggcccga accgatctcg
     6601 cgatcgtcgg cgccggcccg gccggactgg ccgccgccgt caccgccgcc ggcctcgggc
     6661 tgcgggtcgc cctgctcgac gcgggcgacc gtcccggcgg ccagttctac cgccaccccg
     6721 ccccgggact gggcgcggcc cgccccgagg cgctccacca cggctgggcc gatttcgcga
     6781 cgcgcgaagc cgccctgcgc gcgcacgagt cggccggccg gatcacgtac ctcccgctcc
     6841 accacgtgtg ggcggtcgtg ccggccgacg cgggcgaggc gggggaggcg gccggggccg
     6901 gccgcgggga tgccgcgtgg atcctgcacg ccgtcgccgg tcacgccccc gaggagcggg
     6961 ccgcggccgt cacggcccgg gcggtgctca tcgccaccgg cgcctacgag cggcaactgc
     7021 cctttcccgg ctggaccctg ccgggagtgg tcggggccgg cggggcgcag gccatgctca
     7081 agagcgggct ggtgctgccg ggcaagcggg tggtcgtcgc cgggagcggg ccgctgctgc
     7141 tcgccgtggc cgggtcgctg gccgccgccg gagcgaccgt gcccgccgtg gtggaggccg
     7201 ccgcctacac cgcgtacgcg ggccgggtcc ccgccctgct gcgcaacccc ggcaagctcg
     7261 tcgaggcggc cggctacggc ggggcgctgg cccgccacca cgtccggctg ctgacccggc
     7321 acgccgtcac cgaggcccac ggcaccgacc gggtcgaggc cgtcaccgtg gcccggctcg
     7381 accgggactg gcgtccgctg cccggcaccg gccgccggat cccgtgcgac gccctggccg
     7441 tcggccacgg gctggtgccc cagctggaac tcgccaccgg cctgggctgc gccaccgtgc
     7501 gaagcgccga cggcgccgtc gccctcgccc tcgacgccga gcagcggacc tccgtccccg
     7561 ggatctggtc ggcgggcgag accggcggta tcggcggagc ccaactggcc atgatcgagg
     7621 gcgaactggc cgcgcactcc gtcgcgggcg cgctgcccgg ggcccgcctc acccgccgcc
     7681 gcgcgcggct gcgcgccttc gccgacgcga tggccgccgc gcaccgcccc ggcgcgggct
     7741 ggaccggctg gctccgggac gacgcggtcg tctgccgctg cgaggaggtg ccggccgggc
     7801 ggatccggga ggcggccgag gacctcggcg cgcgggacgc ccgtaccgtc aagctcctca
     7861 cccgggccgg catgggctgg tgccagggcc ggatgtgcgg tcccgccgtc gccgccctgg
     7921 ccggggagga agccgcggcc gaccggcgtc cgctgtcctg tccggtgccg ctgcgccagc
     7981 tcgccgaact tccgcccgcc gggaactgat cgggacgcgc gggcccttgt ggccgcgcca
     8041 cacccactag taaaatgtca cacaccactc tagggagctc tcacatgacc cacgcgcaca
     8101 cccccgcgcc ccgcacccgc ccctggcacg gaatcatggt cgccaccgcg ctcccgctgc
     8161 gcgaggacct caccgtcgac tacgacgctt acgccgaaca cgtggcctgg ctgatcgcca
     8221 acggctgcga cggcgtggtc cccaatggtt ccctcgggga gtaccagacc ctcaccgacg
     8281 aggagcgggc gcgcgtcgtg cggaccgccg tcgaggcggc cggcgacggc gcccgcgtca
     8341 tgcccggcgt cgccgcctac ggcagcgccg aggcccgccg ctgggccgag gaggccgccg
     8401 aggccggcgc aggctccgtc ctcctgctgc cccccaacgc cttccgggcc gacgaggaag
     8461 ccgtacgcgc ccactacgcc gaggtcgccc gcgccgggct gcccgtcgtc gcgtacaaca
     8521 accccatcga caccaaggtc gacctgaccc cggccctgct cgcccggctg cacgccgacg
     8581 gcagcatcgt cgcggtcaag gagttcagcg gggacgtgcg ccgggcctac gagatcgccg
     8641 agctggcccc cggtctcgac ctgctcatcg gcgccgacga cgtcctcctc gaactcgccc
     8701 tcgcgggcgc cgtcgggtgg atcgccggct accccaacgc gctcccgaac gcctgcgcca
     8761 ccctctaccg ggccgccgtc gccggggacc tcgacaccgc cctgccgctc tacaagtccc
     8821 tgcactccct gctgcgctgg gactccaaga ccgagttcgt ccaggccatc aagctctcca
     8881 tggacctggc cgggcgcccc ggcggcgcca cccgcccgcc ccgcttcccg ctcacgggcg
     8941 agaccgaggc cgccgtccgc gccgccaccg aaaaggccct cgccgagggc ctcaactagt
     9001 caccgggggg aatccggaat gcgtacgcgc cacgtctacc acgcggtgga ctcgcacacc
     9061 gagggcatgc cgacccgggt catcaccggg ggagtcgggg tggtccccgg cgccaccatg
     9121 gccgagaagc ggctccactt catcgagcac atggaccacg tccggaccct gctcatgtac
     9181 gagccgcgcg ggcactccgc gatgagcggc gcgatcctgc agcccccgac ccgcccggac
     9241 gccgactacg gcgtcctcta catcgaggtg tcgggcctgc tccccatgtg cgggcacggc
     9301 accatcgggg tcgccaccgt cctcgtcgag accggcatgg tgccggtcgt cgagccggtc
     9361 accaccgtcc ggctcgacac cccggccggg ctggtgagcg tcgacgtccg ggtcgaggac
     9421 ggggcggcca ccgccgtcac cctcaccaac gtcccctcct tctgcgtcgg cctcgacctc
     9481 aagaccgacg tgcccggctt cggcacgatc acctacgacc tggcctacgg cggcaacttc
     9541 tacgccttcg tcgaactcga cgccctgggc ctgcccttcg accgagaccg gggggacgac
     9601 ctgatcgccg ccgggctcgc ggtgatggac gcgatcaacg ccggcccgga ccggcccgtc
     9661 caccccgaga acccctccat cgccggggtc aagcacgtct acctcgccgc cccgggctcc
     9721 gacgcccacc gctcccggca cgccatggcc atccaccccg gctggttcga ccgctcgccc
     9781 tgcggtacgg ggaccagcgc gcggatggcc cagctgcacg cccgcggcct gctggagctc
     9841 ggcgccgact tcgccaacga gtccttcatc ggcaccgagt tcaccggacg gctggtcggg
     9901 gagaccaccg tcggcggcct gccggccgtg gtccccacgg tcaccgggcg ggcctggatc
     9961 accggcaccg cccagtactt cctcgacccg accgacccct tccccggagg gttcctgctg
    10021 tgatcaccgt ccgcacggtg gactaccaca ccgccggcga gcccttccgg atcgtcgact
    10081 ccgccgcgac cggcatgccg ccggtccccg gggacaccgt cgccgagcgc tgcgccaccg
    10141 tgatcggccc cggcgggtcc gggacggcgc cgcgccgggg cgcactggac gacgtccggc
    10201 gcctgctggt gcaggagccg cgcggacacg ccgggatgta cggggggttc gtggtcccgg
    10261 ccgacgacga cggggcccac ttcggcgtgc tgttctggca caaggacggc tactccacgg
    10321 cctgcggcca cggcacgatg gccctcgggg cctgggccgt ggacaccggc agggtcgccg
    10381 ccccggacga cggggacgcc ccggtgcgca tcgacgtacc gtcgggacgg gtcaccgcca
    10441 ccgtccaccg cgcggcgggc cgcaccaccg gggtcacctt ccgtaacgtc ccggccctgg
    10501 tgggcgcccg caaggtgccc gtcgccacct cgttcgggct cgccgaggtg gacatcgcgc
    10561 acgccggggc ctgttacgcc gccgtccgcg cgcgcgacct cggcctggac gtgtcgcggg
    10621 cggcgctgcc ctccctggtg cgggccgggc aggagatccg ggcggcgctg gccacccacc
    10681 ccgccacctg gcacccgggc ggcccgctgc tgtccggggt ctacggggtg atcctccacg
    10741 aggagctgcc cgacaccccg ttcgggcctc accagcgcaa tgtcaccgtc ttcgccgacg
    10801 ggcagatcga ccgctcgccc tgcggttccg gcacctcggc acggctcgcg ctgctcgccg
    10861 aggacgggcg gctcggcccc ggcgaggacc tgctccacga gtcggtcacc ggcaccgtgt
    10921 tcaccgggcg gatgctggag ggcggggtta ccgaggtcac cggcaccgcc caccgcaccg
    10981 gcgaacacgc cttcgtgctg gacccgcacg acgcgctcgg caccggcttc ctgctgtgac
    11041 ggacatcctg cggctcgacg ccgggcggat ggccgggctg ctcaccccgg ccggggccgc
    11101 cgacgccctg gccgccgccc ttcgcggcgg cctcgacccg gagagctgcc cgccgcgctc
    11161 ggcgatcgcg gtgcccggcg ggggtgaact gctgctgatg ccggccgcgt ccggcgcgta
    11221 cgccggggtg aagatcgccg gggtcgcgcc cgggaacccg gaccgcggac tgccccggat
    11281 caccggctcc tacctgctgc tggacggccc caccctgcgc ccgctggccc tgctcgacgg
    11341 cgcggcgctg accaccctgc gcaccccggc ggtctccgcc ctcgcgctgc ggcacctggc
    11401 ccccgccggg cgcccgttgc ggatggtgct cttcgggtcc gggccgcaag cgtacggcca
    11461 tctggaagcg gtgctcgccg tgcgggagtt ggccgaggtg gtggtggtcg cccggcatcc
    11521 ggggagggcg gcggagctcg ccgcgcacgc ccgtaccctg ggcccggccg cccgcaccgg
    11581 cacggccggg gaggtggccg gtgccgatct ggtgatctgc tgcaccacgg cccgggaacc
    11641 cctcttcgac gggcgcctcg tcgggccggg cgccaccgtc gtcgccgtcg gctcgcacga
    11701 gccgacggcc cgggagacgg acaccgccct tgtgcgacgg gcggcggtgt acgtggagtc
    11761 ccgggctgcg gccctgcgcg aggccgggga cctcctcgtt ccggaggccg agggcgccat
    11821 cgggcccggt catatcaccg ccaccctcgc cgacctggtg ggcggccgga taccggcggg
    11881 aggcgggcag agttgtccac agctcttcaa gagcgtgggc atggcctggg aagatctcgc
    11941 tgtggcggtc gcgctggtcg aggccgccgg agtgtgagca gcgcaacgtg acattgtacg
    12001 ctgttcctct cctcgtcggt ggtgtacggg ggtctcggag gaaaagcaat gggtgacctg
    12061 aagcagcaca gtctcatcaa ggcccaggaa cgactccgcg accaggtcgg ccacgcactc
    12121 cgagccgcgc tgatagcggg tgagctgcgc ccggggagcg tctactccgc acccggcctc
    12181 gcggccgaac tcggcgtctc ggccacaccg gtgcgcgagg ccatgctcga cctggcccgg
    12241 gagggtctcg tcgagccggt ccgcaacaag ggcttccgga tcaccgaggt cagcgagcgc
    12301 gacctggacc agttcaccga actgcgcacg atgatcgagg tgccgaccat cggccggatc
    12361 acgaagatcg ccacggccga gcagctggag gccctgcgcc ccatcgccga ggagatcgtc
    12421 agcagcgcgc gcgagcacaa cctcatcggc tacctggagg ccgaccgccg cttccacctg
    12481 tccctgctgg ccctcgcggg caacgaccgg ctcgtggaga cggtcggcga cctgcgcaag
    12541 cgctcccggc tgtacggcct gaccggcctg gacgaggcgg gcaagctggt ctcctcggcc
    12601 gaggagcaca tcgaactgct cgacctgatg atcggcggcg acgccgaggc ggcgcaggag
    12661 tgcatgctcc gccacctcgg ccacgtccgc tcgctctggg cccagggccg cgacgaaccg
    12721 gtcggccgcg tacccggcgg cctcggctcg ggcctccagt aggcagccgg cggagcgggc
    12781 tcccggcagg gtgaacggcc gccgccgtcg cgctgccggg tccggcccgc ggagatgacc
    12841 gccccctccc tcacggcacc cccgccgcgg catgcgcgcg cgccgcggtt cttggacacg
    12901 gctggataac ggcggcgcca accccttgtc agtacaattt cacattacta tgttaccggt
    12961 cacatcctag agcgcggccc ttcactggag tgaccatgac caagcgcac
//
