LOCUS       NZ_CP020039            10972 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. 3211 isolate 3 chromosome, complete genome.
ACCESSION   NZ_CP020039
VERSION     NZ_CP020039.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 3211
  ORGANISM  Streptomyces sp. 3211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Complete genomes of three Streptomyces isolated from disease
            suppressive soils
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2017) Biotechnology Institute, University of
            Minnesota, 1479 Gortner Ave, Saint Paul, MN 55108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JAN-2017
            Assembly Method                   :: PacBio v. Jan-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:18:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,254
            CDSs (total)                      :: 8,154
            Genes (coding)                    :: 7,815
            CDSs (with protein)               :: 7,815
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 206 of 340
            Pseudo Genes (incomplete)         :: 257 of 340
            Pseudo Genes (internal stop)      :: 68 of 340
            Pseudo Genes (multiple problems)  :: 153 of 340
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:04:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1444079
            Orig. end                         :: 1455051
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Michael Smanski.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10972
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="11"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10972
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10972
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5972](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5972
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(301..310)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     misc_feature    305..318
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.56"
                     /tool="antismash"
     gene            374..1603
                     /locus_tag="B4U39_RS06800"
     CDS             374..1603
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828668.1"
                     /locus_tag="B4U39_RS06800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_079404209.1"
                     /transl_table=11
                     /translation="MTTRTRTRALAAGDTELKEFTERLFGHLPRADQRRWARVYLQGLL
                     TTPGKKSVRRLAASVTESPTASQSLQQFINASPWDWNPARTELLRWVEERHPVRAWTIG
                     QVCFPKRGEHSVGVHRRFDPAAGRIVNCQLGFCLFLSLDGMNVPVDWRLALPEAWVADP
                     VLRRRARIAPEAVHQSPEALVLELADSIAARTSSARPPVVADLSRLGGAAALIGMLGRR
                     GHDFLVSVPPTLAVRAAGLTPGGDSARTPGRGTTAADFSRLGNTSHPYGTPLAGPGARP
                     QGQRIHSALVRVPEGEPGAGSPAGAQRIYRLFGERRPGAGGRSAGGGGGGGGPVWLTNM
                     NRHRMDDLLGLVRAAGRSAAVLGALADDFGLLDFEGRSFPGWHHHMTLMSAAYAFALRG
                     HVGLPRAQPA"
     misc_feature    455..457
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1736..3103)
                     /locus_tag="B4U39_RS06805"
     CDS             complement(1736..3103)
                     /GO_function="GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155914.1"
                     /locus_tag="B4U39_RS06805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="WP_079404210.1"
                     /transl_table=11
                     /translation="MPDLSNIVKAYDVRGVVPDEWDESLAELFGAAFVEVTGADAIVIG
                     HDMRPSSPGLSGAFARGAAACGVDVTLIGLCSTDQLYYASGALGLPGAMFTASHNPAQY
                     NGIKMCRAGAAPVGQDTGLSQIRELVEKWSEEGAPAVAPGTVAGTVRELDSLPGYAAHL
                     LGLVDLSSIRRLKVVVDAGNGMGGHTVPTVFEGLPLDVVPMYFELDGTFPNHEANPLDP
                     KNIVDLQARVLAEGADLGIAFDGDADRCFIVDERGVGVSPSAITALVAARELARNGGSG
                     TVIHNLITSWSVPEVVRENGGMPVRTRVGHSFIKEEMARTGAIFGGEHSAHYYFKDFWN
                     ADTGMLAALHVLAALGGQDGPLSELVASYDRYAGSGEINSTVADQGARLAAVKAAYGST
                     EGVTLDELDGLTVTAEDWWFNVRASNTEPLLRLNVEARDEATLAKVRDEALSLIRA"
     gene            complement(3209..4165)
                     /locus_tag="B4U39_RS06810"
     CDS             complement(3209..4165)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="B4U39_RS06810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_107469936.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 9.6e-11, bitscore:
                     40.1, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTRRIVVTGATGFVGSAVLRRLAAQDPRAVVRATSRTPPPAATH
                     GVRWVDADLADEPSLRGVCEGADVLLSLASYIGPDADRCAAVNSAGAAALVAEARRAGV
                     ERIVHLSTCAVYGPGPHRGQDVAELTPAPVSPASAGRLAGEGPVLDAGGLVLRAGLVLG
                     TGDRWAVPALVDAFRRVPARWGGGEGLASFVDVDDLARLIVAFALGGGAAAGTTGVLHA
                     HHPEPVRNGDLMDALAEHRVLSARPEEDWPWHRCLEALAATPGWVSERQFTLLARDHWY
                     RADAAWALAGITPGPGPLSRIGPAAAWYRERGGAAPG"
     gene            complement(4162..4899)
                     /locus_tag="B4U39_RS06815"
     CDS             complement(4162..4899)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979399.1"
                     /locus_tag="B4U39_RS06815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_079407768.1"
                     /transl_table=11
                     /translation="MSERKQQRAPQLRAVQTKAAILRAAAEVFDEFGFSGASISKIMKR
                     ADVTQGGMYFHFSSKEELAYAVMVGQGEGLEFPAGEDGLQHLVDITLYLAEQLQHNPVL
                     RAGVRLAVEQGEFGLRDDVAYQAWVLEFRQQLRFARAKGELQPDVDDHELAWVLVSSFT
                     GAQLFSQASTGRADLPQRIASLWRYLLPAVAADGIRGGLRLTLPPTGPKPKNAPKNAPK
                     NAPKNAPTNAPTNETGSGAEKRA"
     misc_feature    4913..4926
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.51"
                     /tool="antismash"
     misc_feature    4914..4927
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.43"
                     /tool="antismash"
     misc_feature    complement(4921..4930)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     gene            5001..5972
                     /locus_tag="B4U39_RS06820"
     CDS             5001..5972
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="B4U39_RS06820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_159395555.1"
                     /sec_met_domain="AfsA (E-value: 7.5e-22, bitscore: 76.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLSTDHSASPIAALSSALPREYVHKSAHSEVLLTGWRTVAPDEY
                     VVTAQWPRTHSFYTPDGGHHDPLLLAESVRQTIPLLSHVAYEVPFGHRQIWDAFSYSTN
                     PEALAVGSTPADIALHIRCSGISRRGRRLAGLTMHVTATRDGDLLGTAEAGFTNQSEAV
                     YRRLRGGNADLDEVAARTIPLPPPLTPRRVGRDRFHDVVLSPTSSTRRAQLRADVNHPI
                     LFDHPVDHAPGMLLLEAVRQAAYRSAFPRRGVLTDMEIRFFRYAELTSACWIETVPIAG
                     DSTTPDRHPVRVVASQNGEVVFTATATINATAPTPLRVHATP"
     misc_feature    5055..5057
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6187..6552)
                     /locus_tag="B4U39_RS06825"
     CDS             complement(6187..6552)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137673.1"
                     /locus_tag="B4U39_RS06825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="WP_237296656.1"
                     /transl_table=11
                     /translation="MTENPLPPCPECAGAYTYELGALLVCPECGHEWPHASGESDGSGG
                     AAEERVIKDAVGNVLADGDTVTVVKGLKVKGSPSGIKAGTKVRNIRLVEGVDGHDIDCR
                     IEGFGPMQLKSSVVRKV"
     gene            complement(6545..7282)
                     /locus_tag="B4U39_RS06830"
     CDS             complement(6545..7282)
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565751.1"
                     /locus_tag="B4U39_RS06830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_107469937.1"
                     /transl_table=11
                     /translation="MSSPSTPARGRPARLDRTRTVDTALDLLDEVGLDALTMRRLADAM
                     DVQAGALYRYFATKGDLLTAMAERMLADLAATAATTATAAAKTEVAGDWSGELAALARA
                     MRTALLARRDGARVYAGTHATGPHTLGFAEAVIGVLRTAGFGAEEAARTLMAVVHLTIG
                     HTLEEQAVLRPVGSEGAADPERLRLAADPERYPQLAAVLPTLTSEDFAAHFEFGLSLLL
                     RGLGTLDRAPTCGDAPEIYSRGD"
     gene            7347..8966
                     /locus_tag="B4U39_RS06835"
     CDS             7347..8966
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151698.1"
                     /locus_tag="B4U39_RS06835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_079404214.1"
                     /transl_table=11
                     /translation="MDTDVIVVGSGPTGLLLACELALAGVRTLVVERRTEPQQDSRALT
                     LHPRSLELMDLRGLAPRFLGLGRTLPGWHFAALETPLDFAALDSRLAHTLFLAQARTEE
                     LLAQRARELGVEIRRGHTLTGLRQGEDGVEAEVRGPQDGAGDGPGDRPGHGPGHRPGHG
                     PGPRPDHGSAGAPRTLRAAYLVGCDGGRSTVREAAGIDFPGTDETLTGVLGDFATVTAG
                     PEALAAARGAGVLAVPLEGGLTRIVYIDPERMRTPAQVPVTLEEFRSALVRLCGSDFGV
                     DRPRWLSRFGNATRLAAAYRSGRVLLAGDAAHIHFPAAGQGLNTGLQDAMNLGWKLAAE
                     VKGWAPPGLLDTYHAERHPVGRAVTDNTEVQTLLAELPLVDRYRRPAAALRGLFEELLR
                     MPEVNRRFAEQVSALGTAYPATAPGADPLVGRRMPDIALSAPGSDATRVYELLHRGGFV
                     LLRLAGARDPHPEPYEAPARHPHISAVTAHAYEEHPELDGVREVLVRPDGHIAWATRSA
                     DAGLRRTGREAALTAWAVRSPA"
     gene            complement(8935..9759)
                     /locus_tag="B4U39_RS06840"
     CDS             complement(8935..9759)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049992.1"
                     /locus_tag="B4U39_RS06840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_079404215.1"
                     /transl_table=11
                     /translation="MTSSDLGDFLRARRAHLRPGDVGLASYGRRRVAGLRREEVAVLAG
                     MNSDYYARLEQGRERGPSPQVLDAISAALRMDEAAREHLFRLAGTAPGGDRPQPREAVA
                     ASLRQLLDGYTSTPAFVLNPATDLLAANALADALFSPFAQRDNLARMTFLDPAARRFFV
                     HPDRAAEAVVAGLRHATGLDPHYPRLRDLVRELGSASAEFAALWSAHTVRGKTYEAKEL
                     DHPDAGRLSLTFQSFDVRGAPGQQLVVYHAEPDSPSARALARLTPATAPPMR"
     misc_feature    complement(10225..10237)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 20.41"
                     /tool="antismash"
ORIGIN
        1 tgcggtcgaa ttcagcggcg gccaccgaga tcagatgatt acgggtgcgt acgccgcgct
       61 cttgcttcac cactgcgcta tcactgccat ccaggtcgtt cctcactcgc catgggaccc
      121 cgcggggaga tggcgacctc cagcgtcacc cgggggcggg ccccccacaa cgccgacttg
      181 acgaagaagc gaccatgccc cgcgctcccc cgcagctccg cgccggcccc gtgaccggtt
      241 ccgccgccct cccccaacca acgtgccgaa gtcgcgggga ggacgccata cgttcgctag
      301 aaaaaaaacg aacgttctgt attctacgag taccgacggc gcctgccgac atccgcactg
      361 gagggggaga cagatgacga cacggacaag gaccagagcc ctggctgccg gtgacaccga
      421 actgaaggag ttcacggagc gcctcttcgg gcacttaccg agagccgacc aacgacgctg
      481 ggcccgggtc tacctgcaag gactgctcac caccccgggg aagaagtccg tacgcaggct
      541 cgccgcgtcc gtcaccgagt cgccgaccgc ctcccagtcc ttgcagcagt tcatcaacgc
      601 cagcccctgg gactggaatc cggcccgcac cgagctactg cgctgggtgg aggaacggca
      661 cccggtacgg gcctggacga tcgggcaggt ctgcttcccc aagcgcggtg agcactcggt
      721 cggcgtgcac cgacgcttcg atcccgccgc cggacgcatc gtcaactgcc agttgggctt
      781 ctgcctcttc ctctccctgg acggcatgaa cgtgccggtc gactggcggc tggccctccc
      841 cgaggcgtgg gtagcggatc ccgtcctgcg ccgccgggcg cgcatcgcgc ccgaggccgt
      901 ccaccagagc ccggaagcct tggtgctgga gctcgccgac tcgatcgcgg cccgcacctc
      961 ctccgcgcgt cccccggtgg tcgccgacct cagccgcctt ggcggggccg ccgcgctgat
     1021 cggcatgctc ggccgccgcg ggcacgactt cctggtgtcc gtaccgccga ccctcgcggt
     1081 gcgcgcggcc ggcctgacgc ccggcggcga cagcgcccgg acgcccgggc gcgggaccac
     1141 cgcggccgac ttctcccgcc tgggcaacac cagccacccg tacggcacac cgctcgcggg
     1201 acccggcgca cgaccgcagg ggcagcgcat ccactcggcg ctggtcaggg tgccggaggg
     1261 ggagccgggg gcggggagcc ctgcgggcgc gcagcggatc taccggctgt tcggggaacg
     1321 gcggccgggg gcgggcgggc ggagcgccgg tgggggtgga ggtggaggtg ggccggtgtg
     1381 gctgaccaat atgaaccggc accggatgga cgacctgctc ggcctggtgc gggcggcggg
     1441 gcggtccgcc gcggtgctcg gggcgctggc ggacgacttc gggctcctcg acttcgaggg
     1501 gcggtccttc cccggttggc accatcacat gacgctgatg tcggccgcgt atgcgtttgc
     1561 cttgcggggg catgtggggc ttccccgggc gcagcccgcc tgagggggcg ggccgcggcc
     1621 cgggggatcc cggctccgcc gggcaccggg ctccgcccgg accccgcgcc tcaaacgccg
     1681 gcgaggctgg gacgggcgga gcccggagtg ccggccgggc cgttgagcaa caggctcagg
     1741 cgcggatgag ggacagggcc tcgtcgcgga ccttggccag ggtggcctcg tcacgggcct
     1801 ccacattcag gcgcagcagc ggctccgtgt tcgaggcacg gacgttgaac caccagtcct
     1861 ccgccgtcac cgtcagaccg tccaactcat ccaaggtcac gccctcggtg ctgccgtagg
     1921 cggccttcac cgccgccaga cgcgccccct gatcggcgac cgtcgagttg atctcccccg
     1981 accccgcgta acggtcgtag gaagccacca gctccgacaa cggcccgtcc tgaccaccca
     2041 gcgccgccag cacatgcagc gccgccagca tccccgtgtc cgcgttccag aagtccttga
     2101 agtagtagtg cgccgagtgc tcaccaccga agatcgcacc cgtcctcgcc atctcctcct
     2161 tgatgaagga atgacccacc ctggtgcgca ccggcatccc gccgttctca cggaccacct
     2221 ccggcaccga ccacgaagtg atcagattgt ggatcaccgt gcccgaacca ccattgcggg
     2281 ccagctcacg cgccgcgacc agcgccgtga tcgccgacgg cgacaccccc acaccccgct
     2341 cgtccacgat gaaacaccgg tccgcgtcac cgtcgaacgc gatacccaga tccgcaccct
     2401 cggcaaggac acgggcctgg agatccacga tgttcttcgg atccagagga ttcgcctcgt
     2461 ggttcgggaa cgtgccgtcc aactcgaagt acatcggcac cacatccaac ggcagaccct
     2521 cgaacaccgt cggaaccgtg tgaccgccca tcccgttgcc cgcatccacc accaccttca
     2581 ggcggcggat cgaggacagg tccaccagac ccaacaggtg cgccgcataa cccggcaggc
     2641 tgtccagctc cctgaccgtc cccgccaccg tgcccggcgc caccgccggc gcgccctcct
     2701 ccgaccactt ctccaccagc tcacggatct gcgacagacc cgtgtcctga cccaccggag
     2761 ccgcacccgc acgacacatc ttgatcccgt tgtactgcgc aggattgtgc gacgccgtga
     2821 acatcgcacc cggcagaccc aacgcacccg acgcgtaata caactgatcc gtcgaacaca
     2881 gaccgatcaa cgtcacatcc acaccacacg ccgccgcacc ccgcgcgaac gcccccgaca
     2941 accccgggga agacggccgc atgtcatgac cgatcacgat cgcatcagca cccgtgacct
     3001 cgacgaacgc cgcaccgaac aactcggcca acgactcgtc ccactcgtcc ggcacgacac
     3061 cacgcacgtc atacgccttg acgatgttcg aaagatcggg catttccact cctccagggg
     3121 tgaccggttg tgctccggac gctaccgctc gccctgcccg gcacagcccg cccacgcccc
     3181 atcccgccga ggctttggca gatagccgtc accccggggc cgccccgccc ctctcccggt
     3241 accacgccgc cgccggaccg atccggctca gcgggcccgg cccgggcgtg atcccggcga
     3301 gtgcccaggc cgcgtccgcg cggtaccagt ggtcccgggc cagcagtgtg aactggcgtt
     3361 cgctcaccca gcccggggtg gcggccagcg cctccaggca cctgtgccag ggccagtcct
     3421 cttcgggccg cgccgacagc acccgatgct cggcgagcgc gtccatgagg tccccgttgc
     3481 gtaccggctc cgggtggtgg gcgtgcagga cacccgtcgt cccggccgcc gcgccgccgc
     3541 cgagggcgaa ggcgacgatg agccgggcca ggtcgtcgac gtcgacgaag gaggccagcc
     3601 cctcccctcc gccccatcgc gccggtaccc gccggaaggc gtcgacgagg gccggtacgg
     3661 cccaccggtc gcccgttccg agtaccagtc ccgcgcgcag gaccagtccc ccggcgtcga
     3721 gcacgggccc ctcacccgcc agccggccgg cgctggccgg cgagaccggg gcgggtgtca
     3781 gctcggcgac gtcctgtccc cggtgcggtc ccggtccgta cacggcgcag gtcgacaggt
     3841 ggacgatccg ctccactccg gcccttcggg cctcggccac cagggccgcg gcgccggcgg
     3901 agttgaccgc ggcgcaccgg tcggcgtccg gtccgatgta ggaggccagc gacagcagga
     3961 cgtccgcgcc ctcgcacacc ccgcgcaggg acggctcgtc ggccaggtcc gcgtccaccc
     4021 agcgcacgcc gtgcgttgcc gcggggggcg gcgtacgcga cgtggcgcgc accacggccc
     4081 gcgggtcctg cgcggccagg cggcgcagca ccgccgatcc cacgaagccc gtcgctcccg
     4141 tgacgacgat gcggcgggta ctcaagcccg cttctccgcg cccgacccgg tctcgttcgt
     4201 cggcgcgttc gtcggtgcgt tcttcggtgc gttcttcggt gcgttcttcg gtgcgttctt
     4261 cggcttcggc ccggtgggcg gcagtgtcag gcgcagcccg ccgcggatgc cgtcggcggc
     4321 caccgcgggc agcaggtagc gccacagcga ggcgatccgc tgcggcaggt ccgcccgtcc
     4381 cgtggacgcc tgggagaaga gctgtgcccc ggtgaaggag ctgacgagca cccaggccag
     4441 ttcgtggtcg tcgacgtccg gctggagctc ccccttggcc cgggcgaagc gcagctgctg
     4501 gcggaattcc agcacccagg cctggtaggc gacgtcgtcg cgcaggccga actcgccctg
     4561 ttccacggcc agtcgtacac cggcccggag caccggattg tgctggagct gttcggcgag
     4621 gtagagcgtg atgtccacca ggtgctgcag gccgtcctcc ccggcgggga actccaggcc
     4681 ctctccctgg ccgaccatga cggcgtaggc gagttcctcc ttcgagctga agtggaagta
     4741 catgccgccc tgcgtgacgt ccgcacgctt catgatcttg ctgatgctgg ccccgctgaa
     4801 gccgaactcg tcgaagacct cggccgcggc gcgcaggatg gcagctttgg tctgaactgc
     4861 cctgagttga ggggcgcgtt gctgcttccg ttctgacatg agcacctaac taatctcgac
     4921 aaaaaaaacg aacatccttt attttaggac ccgcgccgaa caagcaccag ctcatgtccg
     4981 acgtccaggg gggctcaacc atgactctgt ctactgatca ctccgcctca cccatcgccg
     5041 cgctcagcag cgccttaccg cgggagtacg tacacaagag cgcacactcg gaagtccttc
     5101 tcaccgggtg gcgcaccgtc gcgccggacg agtacgtcgt caccgcccaa tggccccgca
     5161 cccacagctt ctacacgccg gacggcggcc accacgatcc cctgctcctc gccgagtccg
     5221 tccgccagac catcccgctc ctgagccacg tcgcgtacga agtccccttc ggtcaccgcc
     5281 agatctggga cgccttcagc tactcgacca accccgaggc tctcgccgtc ggttcgaccc
     5341 cggccgacat cgcactgcac atccgctgct ccggcatctc gcggcgcggc cgaagactcg
     5401 cgggtctgac catgcacgtg accgccacgc gcgacggaga cctcctcggc accgccgagg
     5461 ccggcttcac caaccagtcc gaggccgtct accggcgcct gcgcggcggg aacgcggacc
     5521 tcgacgaggt ggccgcccgc accatcccgc tgccgcctcc gctgacaccc cgccgcgtgg
     5581 gccgcgaccg cttccacgac gtggtcctct cccccaccag ctccacgcgg cgcgcccagc
     5641 tccgggccga cgtcaaccac ccgatcctct tcgaccaccc cgtcgaccac gcccccggca
     5701 tgctgctcct ggaagccgtg cgtcaggccg cgtaccgctc cgccttcccc cgacggggag
     5761 tcctgaccga tatggagatc cgcttcttcc gctacgcgga gctgacctcg gcctgctgga
     5821 tcgagaccgt cccgatcgcc ggcgattcga ccaccccgga ccgccatccc gtacgcgtgg
     5881 tcgccagcca gaacggcgag gtcgtcttca ccgccaccgc gacgatcaac gccacggcgc
     5941 ccacccccct ccgcgtccac gccacgccct gaccgttcgg tgaggcgccc ggctcatgag
     6001 cgcgccggct catgagcccc cgccgaaccg acccgtccct tccctccggc tctccggctc
     6061 tccggctctc cggctctccg gctctccggc tctccggctc tccggaagaa cggcgaaggc
     6121 gcctgatcgg gtccgtaccc gacaggcgcc ttcgctgttc gtccgttccc ggcgtcgccg
     6181 ccccggtcag accttcctga ccacgctgga cttcagctgc atggggccga agccctcgat
     6241 cctgcagtcg atgtcatgcc cgtcgacacc ctccaccagg cggatgttgc gcaccttggt
     6301 gcccgccttg atgccgctcg ggctgccctt gaccttcagg cccttgacga ccgtcacggt
     6361 gtccccgtcg gccagcacat tgcccaccgc atccttgatc acccgctcct cggcggcgcc
     6421 gcccgacccg tcggactcgc cggaggcgtg cggccactcg tgcccgcact cggggcagac
     6481 caggagcgcc cccaactcat aggtgtacgc acccgcgcat tcggggcagg gaggcaaggg
     6541 gttctcagtc accccgcgag tatatttcag gcgcatcgcc gcaggtcggc gcccggtcca
     6601 gggtcccgag cccgcgcagg agcagggaca gcccgaattc gaagtgcgcc gcgaagtcct
     6661 cactcgtgag cgtgggcagc acggcggcga gctgcgggta ccgctcggga tcggccgcca
     6721 ggcgcaggcg ttcggggtcc gccgccccct cgctgcccac cggccggagc accgcctgct
     6781 cctccagcgt gtggccgatg gtcaggtgga ccaccgccat cagcgtgcgc gccgcctcct
     6841 ccgccccgaa tccggccgtg cgcagtaccc cgatgaccgc ctcggcgaag ccgagggtgt
     6901 gggggccggt cgcatgggtg cctgcgtaga cgcgtgcgcc gtcgcggcgg gcgagcagcg
     6961 ccgtacgcat ggcccgggcg agggcggcca gttccccgct ccagtcgccg gcgacctcgg
     7021 tcttcgccgc tgccgtcgct gtcgtcgccg ccgtcgccgc gaggtcggcg agcatccgtt
     7081 ccgccatcgc ggtcagcagg tcgcccttgg tggcgaagta ccggtacagc gcgccggcct
     7141 ggacgtccat cgcgtcggcg aggcggcgca tcgtcagtgc gtcgaggccg acctcgtcga
     7201 ggaggtccag ggccgtgtcg accgtccggg tgcgatcgag gcgggccggg cggccgcggg
     7261 cgggggttga cggtgaactc atggccctca ctatagtgaa cagcgttcac gtgaacgctg
     7321 ttcactaatt gtgggaggca tcaccgatgg ataccgacgt gatcgtcgtg ggctcgggac
     7381 cgaccgggct gctgctcgcc tgcgagctgg cactcgccgg cgtacggacc ctggtcgtcg
     7441 agcggcgcac cgagccccag caggactccc gggcgctgac cctgcatccg cgcagcctcg
     7501 aactgatgga cctgcgcggt ctcgcaccgc gcttcctcgg gctcggccgg acgctgcccg
     7561 gctggcactt cgccgccctg gagaccccgc ttgacttcgc cgcgctggac agccgtctgg
     7621 cgcacacgct gttcctggcc caggcccgta ccgaggagct gctggcacag cgggcgcggg
     7681 aactgggcgt ggagatacgc cgcggccaca cgctcaccgg gctgcggcag ggcgaggacg
     7741 gggtcgaggc ggaggtgcgc ggaccgcagg acggggcggg cgacgggccg ggcgaccggc
     7801 cgggccacgg gcctggtcac aggcctggtc acgggcctgg tccccggccg gaccacgggt
     7861 cggccggggc accgcggacc cttcgcgccg cgtatctcgt gggatgcgac ggcgggcgca
     7921 gcaccgtccg ggaggcggcc gggatcgact tccccggcac cgacgagacc ctgaccggcg
     7981 tactcgggga tttcgccacc gtcaccgcgg gtcccgaggc cctggccgcc gcccgcggcg
     8041 cgggcgtgct cgccgtcccg ctggagggcg gactcacccg catcgtgtac atcgacccgg
     8101 agcggatgcg gacaccggct caggtaccgg tgaccctgga ggagttccgc agcgcgctgg
     8161 tccggctctg cggctcggac ttcggggtgg accggccccg ctggctctcg cgcttcggca
     8221 atgccacccg gctggcggcc gcctaccgct ccggccgtgt cctgctggcc ggcgacgccg
     8281 cccacatcca cttccccgcc gcgggccagg gcctcaacac cgggctccag gacgccatga
     8341 acctcggctg gaaactggcc gcggaggtga agggctgggc cccgcccggg ctcctcgaca
     8401 cctaccacgc cgaacgccac cccgtcggcc gggccgtcac cgacaacacg gaggtgcaga
     8461 cgctgctggc ggagctgccg ctcgtggacc gctaccggcg gccggccgcc gccctgcgcg
     8521 ggctcttcga ggaactgctg cggatgcccg aggtcaaccg ccggttcgcc gaacaggtct
     8581 ccgcgctcgg caccgcctac ccggccaccg cccccggcgc cgatcccctg gtcggccgca
     8641 ggatgcccga catcgccctg agcgcgcccg gttcggacgc gacacgggtg tacgagctcc
     8701 tgcaccgggg cggcttcgtc ctgctccgcc tcgccggtgc gcgggacccg cacccggagc
     8761 cgtacgaggc ccccgcccgg cacccgcaca tcagcgccgt caccgcccac gcgtacgagg
     8821 agcacccgga gctcgacggg gtgcgcgagg tcctggtccg ccccgacggg cacatcgcct
     8881 gggccacccg cagcgccgac gccggcctgc ggcgcaccgg gcgcgaggcg gcgctcaccg
     8941 catgggcggt gcggtcgccg gcgtgagccg ggcgagggcc cgggcgctcg ggctgtccgg
     9001 ttcggcgtgg tagaccacca gctgctggcc cggcgccccg cgcacgtcga aggactggaa
     9061 ggtgagcgag agccggccgg cgtcggggtg gtcgagttcc ttggcctcgt aggtcttgcc
     9121 ccggacggtg tgcgcggacc acagggcggc gaactctgcg ctggccgagc cgagttcacg
     9181 gaccaggtcg cgcagcctcg ggtagtgcgg gtcgaggccg gtcgcgtggc gcagtcccgc
     9241 caccaccgcc tcggccgcac ggtcggggtg cacgaagaac cgtcgggccg cggggtcgag
     9301 gaaggtcatc cgggcgaggt tgtcccgctg cgcgaacggg gagaagagcg cgtcggccag
     9361 cgcgttggcc gccaagaggt cggtggcggg gttgaggacg aacgccgggg tgctcgtgta
     9421 cccgtccagc agctgccgca gggaggccgc gaccgcctcc cgtggctgcg gccggtcacc
     9481 gccgggcgcg gtgcccgcca gcctgaacag gtgttcccgc gcggcctcgt ccatgcgcag
     9541 ggcggcgctg atcgcgtcga ggacctgcgg ggagggtccg cgctcgcgcc cctgttccag
     9601 ccgggcgtag tagtcgctgt tcattccggc caggacggcg acctcctcgc ggcgcagccc
     9661 tgccacgcgg cggcggccgt aggaggcgag tcccacgtcg ccgggccgca ggtgcgcgcg
     9721 ccgggcgcgc aggaagtctc cgagatcgct gctggtcaca ccttcaggtt agaaggcggc
     9781 tctgcgtggc ggaactgccg ttgtttattg gcgcgttccc gctgcttccg gcgcccgccc
     9841 cacctgatga tcttgcgtag gctcgtgatc gaccgacctg acagaccaga cctgacagac
     9901 ccgaccggac cggaccgacc cgaccgacac cgccgcccga cccgacgggt cgacaccggc
     9961 cgttcgacgc ccggcggacc ggattccgcc cccaccggag gagcatcgtg ccccgaccca
    10021 tgaaccgacg cctggccctc ggcgcagccc tgctgggggt gatggccgcc gtggctccgg
    10081 cgaccaccgc ccacgccgcc ccgaccaccc cggacctcga agcgctgtcc caggcgctgc
    10141 ggaacacgac cgccgccgga gcgccgggcg ccatggcccg tttcagcggt cctgacgggg
    10201 tcagcgtccg gatggagggg gtgcgcgaca aggtcacggg gtcggcgatg gacccgcagg
    10261 cccgcttccg gatcgggagc gtctccaaga ccttcacctc cgtcgtcctg ctgcagctgg
    10321 tggacgaggg caaggtcgat ctggacaccg ccgtgaaccg gtacctgccc gggctgctgc
    10381 ccgacgaccg gatcacggtg cgccacctgc tgagccaccg cagcggcctg tcggactaca
    10441 ccaatgccat gttcgagcac accgtgccgg gcttcgaggc cgtgcgcaac cgggtgttca
    10501 gctaccagga gctggtgaac ctctcgctcg ccgaaccgcg gacgaacgag cccggcgccg
    10561 cgtacgcgta ctcgaacacc aacttcgtcg tcctcggcat gctcatcgag aaggccaccg
    10621 gccacggcgt ggccaaggag tacgagcggc gcatcttcaa gccgctgaag ctgcgcaaca
    10681 cctcctacgt gcaccccggg acgcagatca gggggctgca cgcgcacggc tacctccacc
    10741 ccgacgaggc gggcgccccg ctcgtcgact ccaccgagca gacggtgtcg tgggcccagt
    10801 ccgcgggagc ggtgatctcc aaccccgctg acctgaacac cttcacctcc gcgctgctcg
    10861 gcggccggct gctgcccgcg cgcctgctgg acgccatgct caccgcgacc ccgaccgacg
    10921 ccacgaacac ccgcttctac gggctcggcc tgcgccgcta cgacctgtcc tg
//
