LOCUS       NZ_CP020039            10405 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. 3211 isolate 3 chromosome, complete genome.
ACCESSION   NZ_CP020039
VERSION     NZ_CP020039.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 3211
  ORGANISM  Streptomyces sp. 3211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Complete genomes of three Streptomyces isolated from disease
            suppressive soils
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2017) Biotechnology Institute, University of
            Minnesota, 1479 Gortner Ave, Saint Paul, MN 55108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JAN-2017
            Assembly Method                   :: PacBio v. Jan-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:18:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,254
            CDSs (total)                      :: 8,154
            Genes (coding)                    :: 7,815
            CDSs (with protein)               :: 7,815
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 206 of 340
            Pseudo Genes (incomplete)         :: 257 of 340
            Pseudo Genes (internal stop)      :: 68 of 340
            Pseudo Genes (multiple problems)  :: 153 of 340
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:04:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 646026
            Orig. end                         :: 656431
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Michael Smanski.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="7"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    330..344
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.93"
                     /tool="antismash"
     gene            659..1264
                     /locus_tag="B4U39_RS03215"
     CDS             659..1264
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735601.1"
                     /locus_tag="B4U39_RS03215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030387823.1"
                     /transl_table=11
                     /translation="MRLYAQTPARRNRQVLADLIAVGLMAAAVWFALAVHDAIMMLAEP
                     GRRVESSGDSLAGGLADAGDAASNVPFVGDLLKKPLQSAADASSGIADAGQSMQDAVGQ
                     LATLTTVALIVLPVAVVLLLWLPARLRWIRRSVTLRRLFDAPGGADLLALRALTGPLGD
                     LLALPTPPGGLADAWRRGDERVIGELSEAALRRAGLRP"
     gene            complement(1271..1876)
                     /locus_tag="B4U39_RS03220"
     CDS             complement(1271..1876)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061141.1"
                     /locus_tag="B4U39_RS03220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_079403627.1"
                     /transl_table=11
                     /translation="MSSHTTLSPPVVRDHPTRRPREVDNRAAYVSFGLAYVLGHGAAAA
                     SHGAAPVLDLPGWLPMALLGLGLVTGVVLATLAALRAQHGAGAPEILSGKLLGAAWIIG
                     FTALFLAVTGLTSVLDHPDLQSVLWPAGSGLVVGLLYLAEGAVRRNVLHYSLGVWLAIT
                     STAALALAGPGFYWALALGGGGAYALAAALEHRRLHAR"
     gene            complement(1929..2372)
                     /locus_tag="B4U39_RS03225"
     CDS             complement(1929..2372)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (11-144). E-value:
                     4e-23. Score: 74.3. Matches aSDomain:
                     nrpspksdomains_B4U39_RS03225_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /locus_tag="B4U39_RS03225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SRPBCC family protein"
                     /protein_id="WP_079403628.1"
                     /transl_table=11
                     /translation="MHTTIDTTAPVVVRLSTVIDASPATVWELHTDIDDWAAWNDGVDR
                     ARLNGPLEVGSSFTWLTHGLEITSTVLELVPGQRIVWGGTVGGIVGIHVWTFEESGAGV
                     TVRTEESWSGDPVDAAVDELTAALRASLQSWLDSLKARAEQPA"
     aSDomain        complement(1941..2339)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4U39_RS03225_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="4.00E-23"
                     /label="B4U39_RS03225_Polyketide_cyc2.1"
                     /locus_tag="B4U39_RS03225"
                     /protein_end="144"
                     /protein_start="11"
                     /score="74.3"
                     /tool="antismash"
                     /translation="VVRLSTVIDASPATVWELHTDIDDWAAWNDGVDRARLNGPLEVGS
                     SFTWLTHGLEITSTVLELVPGQRIVWGGTVGGIVGIHVWTFEESGAGVTVRTEESWSGD
                     PVDAAVDELTAALRASLQSWLDSLKARAE"
     gene            complement(2449..3942)
                     /locus_tag="B4U39_RS03230"
     CDS             complement(2449..3942)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309675.1"
                     /locus_tag="B4U39_RS03230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_079403629.1"
                     /transl_table=11
                     /translation="MNLGRTKNETEPGAPPRQGAALAALAAAQFAVMLATSIVNVALPQ
                     IRSGVGLSDGGTTWVVNAYGLAFGALLLAGGRAGDLLGRRRVLISGLAVFAAASLAAGL
                     AASPGLLIAARALQGIGAAAIAPAALALAMDLFPPGPGRGRVLGVWGAVSGAGGAGGVL
                     LGGVLTQAWGWPWLFHAVALGTTLVLIAVATLVPRADTGGSGRFDLLGTATVTLALTCL
                     VWALTSARAQGWTDPAVLAALLGAAALLVVFVLVERRRPDALVPARLFTAGRVAAGNLL
                     MALLGSVWIALFFFLPLYQQQVLGMSPLATGAGQLPLAVANMLGALLAPRIAARIGTTA
                     TLITGLLAEAAGLLWLSRMRADGSYLVDALGPSVLVGLGLSLAFVQLTALAVDGVPRQD
                     TGLAGGLVNTTRQVGGAIGLAALATLAGSVTARAATHQPHLEALTAGYRAAFAVSAAVL
                     AATALLAVPLTRPLRRSRRAVPEATPSPQGRAPEHAPAA"
     gene            complement(4049..4951)
                     /gene="thpD"
                     /locus_tag="B4U39_RS03235"
     CDS             complement(4049..4951)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004613.1"
                     /locus_tag="B4U39_RS03235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_053703532.1"
                     /sec_met_domain="PhyH (E-value: 4.7e-38, bitscore: 130.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVSERTLADLYPTRGSEEVLIGRKDPVVWSEPGTPGPFWAREL
                     EDFERDGFLAAEQLIGPEEVEEYRAEMDRLVSDPAVRADERAIVEPGSGEVRSVFEVHR
                     ISEVFARLAADPRIADRARQILGSDVYVHQSRINAKPGFGASGFYWHSDFETWHAEDGL
                     PRMRTVSVSIALTPNHTTNGSLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPTPE
                     ALTRFATACGIRQFTGAAGSATWFDCNAMHGSGDNITPYPRSNVFLVFNSVDNLPAEPF
                     AAPVRRPSFIASRDFTPLP"
     gene            complement(5001..5405)
                     /locus_tag="B4U39_RS03240"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="B4U39_RS03240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_030387828.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.8e-52, bitscore:
                     173.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFQDIEGTDRHVRAASGTWESKRLILAKERVGFSLHETVLY
                     AGTETSMWYANHVEAVVCVEGEAELTDEETGEKHTITPGTVYVLDGHERHTLRVVREFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPDPV"
     gene            complement(5457..6725)
                     /gene="ectB"
                     /locus_tag="B4U39_RS03245"
     CDS             complement(5457..6725)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 3.2e-72.
                     Score: 235.3. Matches aSDomain:
                     nrpspksdomains_B4U39_RS03245_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="B4U39_RS03245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_079403630.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.5e-78, bitscore:
                     262.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTAPIPTVFDTVESEVRSYCRAWPVVFDRAEGSRLHDENGRSY
                     LDFFAGAGSLNYGHNNPVLKQALLDYIARDGITHALDMSTTAKRAFLETFHSTVLAPRG
                     LPYKVMFPGPTGTNAVEAALKLARKAKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHSTPMPFDNYLGGRVPDFLWFERLLEDSGSGLNDPAAVIVETVQGEGGVNVARA
                     EWLRALADLCRRRDMLLIVDDVQMGCGRTGDFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALQTYWRDGVLRERTRVQGARIEQALV
                     DLCADHERDGASYRGRGMVWGLEFTRKERAAEVCHRAFEHGLLVETSGPESEVVKLLPP
                     LTVSADELDEGLDILARSVRETA"
     aSDomain        complement(5631..6641)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4U39_RS03245_Aminotran_3.1"
                     /evalue="3.20E-72"
                     /label="B4U39_RS03245_Aminotran_3.1"
                     /locus_tag="B4U39_RS03245"
                     /protein_end="365"
                     /protein_start="28"
                     /score="235.3"
                     /tool="antismash"
                     /translation="FDRAEGSRLHDENGRSYLDFFAGAGSLNYGHNNPVLKQALLDYIA
                     RDGITHALDMSTTAKRAFLETFHSTVLAPRGLPYKVMFPGPTGTNAVEAALKLARKAKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHSTPMPFDNYLGGRVPDFLWFER
                     LLEDSGSGLNDPAAVIVETVQGEGGVNVARAEWLRALADLCRRRDMLLIVDDVQMGCGR
                     TGDFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATAALQTYWRDGVLRERTRVQGARIEQALVDLCADHERDGASYRGRGMVWGLEFT"
     gene            complement(6862..7422)
                     /gene="ectA"
                     /locus_tag="B4U39_RS03250"
     CDS             complement(6862..7422)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337972.1"
                     /locus_tag="B4U39_RS03250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_079403631.1"
                     /transl_table=11
                     /translation="MTGAHADLATRPRTPAPTTSPSGIRADRPDLCDGPDLWRIARDAK
                     TLDVNSPYSYLLWCRDFAATSAVARDGDGRPLGFITGYLRPDTPATLLIWQVAVDADAR
                     GLGIAGHLLDHLTARVAAEHPLDALETTISPGNTASERLFASFAERHGAQLAREVLFAQ
                     EHFPGGAGHHDAEVLHRIAPLAL"
     misc_feature    complement(7575..7589)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.23"
                     /tool="antismash"
     misc_feature    complement(7588..7605)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.88"
                     /tool="antismash"
     misc_feature    complement(7605..7625)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.54"
                     /tool="antismash"
     gene            7744..8295
                     /locus_tag="B4U39_RS03255"
     CDS             7744..8295
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849917.1"
                     /locus_tag="B4U39_RS03255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_079403632.1"
                     /transl_table=11
                     /translation="MSEQQRPADPSPAADDIDEVTHAVLAASQRLASIAARALAAAGDG
                     GRGGHGVTLAQFRMLEVLVARGHVKLVALAGLMAVNPSTAMRMIDRLIAAGLADRRPNR
                     ENRRETVLTATDSGLRLVGEVTARRRAETAAALARLRPAERTVLIGALGAFAATGPQPA
                     EDGPHAFPDLLGCAQPYAQG"
     gene            complement(8337..9932)
                     /locus_tag="B4U39_RS03260"
     CDS             complement(8337..9932)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037768.1"
                     /locus_tag="B4U39_RS03260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_079403633.1"
                     /transl_table=11
                     /translation="MPSSRIRRAWLSAAAAAALAFTGLAGPATAKDLPPDVPGSWIQEP
                     RATEGAPSAFGARAAAAPGGPASPGDFTLSPGEMNAETTRRVARLASVLPESGVQDLLA
                     AANRPIEPGCSRDPFAAAPDPAVKYCLKDDDSVSREWVPQGFTGVSDAKDNELWGAAGK
                     IQLFASYDGWDPGRETDPNPATGDCTAAELEANDACNQKGVRITFVQSRNNPATGSPEI
                     KYRHVLLGWTYVNSADHVSFDGLHAAEYPIQKGVHAGGIVWYGNYLYVADTRGGLRVFD
                     MRFIMDLDPDGDPATHDRMGTDADGVKTTADVEDKTKVGRHDNVWYSFGYRYVMPQVAA
                     WKFKATQSNPKGSFACVSTGAPKASYISLDRSTGPDRLIMGEYCRPESGYPSTGRIASY
                     PVAALEQRSADVTAQGWANYLPLANGGAQGAAAYNGTLYVNESKGTDEPGNLWRYLWSD
                     GRLVQDGQAVKTARGAEDLYVERGTGRLWSLSEHRPGSEDDCRANPGPGDPEGADFCQR
                     VLYAHELSWLDSQP"
     misc_feature    10204..10218
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
ORIGIN
        1 cgtcctcgag cccgaggccg acgggttccg caacttcctg cgggcgggtg agaagctgtc
       61 gccggaggcg cttctgctgg accgcgccag catgttgaac ctgaccgccc ccgagatgac
      121 ggtgctcgtc ggcggcatgc gggccatgaa caccggcttc cggcagtccc cgcacggggt
      181 gttcacggac cgtccggagg cgctgaccaa cgacttcttc gtcaacttgc tcgacatggg
      241 gacgcagtgg aaggcgtcgg agtcggcgga gaacgtgttc gagggccggg accacgccac
      301 gggcgacgtc aagtggacgg cgaccgcggt ggacctcgtc ttcggttcgc acgcccagct
      361 ccgggcgctc tgcgaggtct acgcgtcgaa ggacgcggga gagaagttcg tccgtgactt
      421 cgtctccgcc tgggacaagg tgatgaacct cgaccggttc gatctcgcct aggccggtcc
      481 gacgttcgac gggccggccg gtcggtgacc ggtcggcccg tccggttccg gcggtgggcg
      541 gttggccggc cggggccggt ggaagggtag agaccggcgt acggtcgacc ggccggccgg
      601 gccccgtccg gtggggtccg tacgtcctcg acgtcctgac tcgaacggga gcccgccgat
      661 gcgcctctac gcacagactc cagcacgtcg gaaccgccag gtgctcgcgg acctgatcgc
      721 cgtgggcctg atggccgccg cggtgtggtt cgccctggcc gtgcacgacg cgatcatgat
      781 gttggccgag ccgggccgca gggtggagag ttccggcgac agcctcgccg gcggactcgc
      841 cgatgccggc gacgcggcct cgaacgtccc gttcgtcggc gacctcttga agaagccgct
      901 ccagtccgcg gccgacgcca gtagcgggat cgcggacgcc ggccagtcga tgcaggacgc
      961 cgtcggccag ctggccacgc tcaccacggt ggccctgatc gtcctgccgg tggccgtcgt
     1021 cctgctgttg tggctccccg cgcgcctgcg ctggatccgg cgcagcgtca ccctgcggcg
     1081 cctgttcgac gcaccgggcg gcgccgacct gctcgccctg cgcgccctga ccggaccgct
     1141 gggcgacctc ctcgcgctcc ccaccccgcc gggaggcctc gccgacgcct ggcggcgcgg
     1201 ggacgagcgc gtcatcggcg agctctcgga ggccgccctg cggcgggccg gcctgcggcc
     1261 ctgacggccg tcagcgggcg tgcaggcggc ggtgttcgag ggccgcggcg agggcgtagg
     1321 cgccgccgcc cccgagggcg agggcccagt agaacccggg gccggcgagc gcgagtgccg
     1381 cggtggaggt gatggcgagc cagacgccga gactgtagtg cagcacgttg cggcgtacgg
     1441 cgccctcggc gaggtagagc aggccgacga cgagtccgga gccggccggc cacaggacgg
     1501 actggagatc cgggtggtcg agtacggacg tcaggccggt gacggcgagg aacagcgcgg
     1561 tgaacccgat gatccaggcg gcgccgagca gctttccgga gaggatctcc ggtgcgccgg
     1621 ccccgtgctg ggcccgtagg gcggcgagcg tggccaggac gacgccggtc acgagcccga
     1681 ggccgaggag tgccatgggc agccagccgg gcaggtcgag cacgggcgcg gcgccgtggg
     1741 aggcggcggc cgcgccgtgg ccgaggacgt aggcgagtcc gaagctgacg taggccgcgc
     1801 ggttgtcgac ctcgcggggg cggcgggtgg ggtggtcacg gacgacgggc ggcgagaggg
     1861 tggtgtggct ggacacggtg gagacctcca tcggggggcg tggcgcggtg gggcgggcgg
     1921 ggtgacggtc aggcgggctg ttcggcgcgt gccttgaggc tgtcgagcca gctctgcagg
     1981 gaggcccgca gggcggcggt cagctcgtcg accgcggcgt cgacggggtc cccgctccag
     2041 gactcctcgg tacggacggt gacgccggcc ccggactcct cgaaggtcca cacgtggatg
     2101 ccgacgatgc cgccgacggt cccgccccag acgatccgct gtccggggac cagttcgagg
     2161 acggtggagg tgatctccag gccgtgggtg agccaggtga agctgctgcc gacctccagc
     2221 gggccgttca gccgggcccg gtcgacgccg tcgttccagg ccgcccagtc gtcgatgtcg
     2281 gtgtgcagct cccagacggt ggcgggagag gcgtcgatca ccgtcgagag gcggacgacg
     2341 acgggggcag tggtgtcgat ggtggtgtgc atgggtgagt ccttttcggg tggccgggtc
     2401 gccggatcat cgggtcgccg gggggacggg cttgggatca gacgtggatc aggcggcggg
     2461 tgcgtgttcg ggcgcccggc cctgcgggga gggcgtggcc tccgggacgg cacggcggga
     2521 gcggcggagc gggcgggtga gcggcaccgc gaggagcgcg gtggcggcca ggaccgcggc
     2581 cgagaccgcg aaggcggccc ggtagcccgc ggtgagggct tccaggtggg gctggtgggt
     2641 ggccgcacgg gcggtgacgg agccggccag ggtggcgagg gcggcgagcc cgatcgcgcc
     2701 gccgacctgg cgggtggtgt tgaccagtcc gccggccagt ccggtgtcct gccgcggtac
     2761 gccgtccacg gcgagcgcgg tgagctggac gaaggcgagg ctcaggccga ggccgaccag
     2821 gacgctcggt ccgagggcgt cgacgaggta gctcccgtcc gctctcatcc gtgacagcca
     2881 cagcaggccc gcggcctcgg cgagcaggcc cgtgatcagg gtggccgtgg tgccgatgcg
     2941 ggcggcgatg cggggcgcga ggagtgcgcc gagcatgttg gcgacggcga gggggagttg
     3001 gcccgcgccg gtggccagcg ggctcatgcc gaggacctgt tgctggtaga gcgggaggaa
     3061 gaagaacagc gcgatccata cggatcccag cagcgccatc aggagattgc ccgcggcgac
     3121 ccggccggcc gtgaacagcc gggccgggac cagggcgtcg ggtcggcggc gttcgacgag
     3181 gacgaagacc acgagcagcg cggcggcgcc caggagggcg gccagtactg cggggtcggt
     3241 ccagccctgg gcgcgggcgc tggtcagtgc ccacaccagg caggtcaggg cgagggtgac
     3301 ggtcgcggtg cccagcaggt cgaaccgccc ggagccgccg gtgtcggcgc ggggtacgag
     3361 cgtcgccacg gcgatgagga cgagcgtcgt gccgagcgcg acggcgtgga agagccaggg
     3421 ccagccccat gcctgggtga gtacgccgcc cagcaggacg ccgcccgcgc cgcccgcgcc
     3481 ggagacggcg ccccacactc cgaggacccg gcccctgccg gggccgggcg ggaacaggtc
     3541 catcgccagg gcgagcgcgg cgggtgcgat ggcggcggcg ccgatgccct ggagggctcg
     3601 ggcggcgatc agcaggcccg gcgaggcggc cagtcccgcc gccagcgagg ctgccgcgaa
     3661 aacggcgagg ccggagatca gcacccggcg gcggccgagc aggtcgcccg cccggccgcc
     3721 ggcgaggagc agtgcgccga acgccaggcc gtaggcgttg accacccagg tggtgccgcc
     3781 gtccgacagg cccactccgg agcggatctg tggcagcgcc acgttcacga tggaggtggc
     3841 cagcattacg gcgaactgcg ctgcggccag ggcggcgagt gcggctccct ggcggggtgg
     3901 ggctccgggt tcggtttcgt tcttcgtgcg tccgaggttc atggcgcaca gcctcgtgtc
     3961 gggcggcgtc cacaatccat gcccgacagg ggcggtcact gtccagtcgt gtccgcccct
     4021 gtccactcgg ccttgccggg tacgtgcctc agggcagcgg cgtgaagtcg cgcgaggcga
     4081 tgaaggacgg gcgccggacc ggggccgcga agggctccgc gggcaggttg tccacgctgt
     4141 tgaacaccag gaagacgttg ctgcgcgggt agggggtgat gttgtccccg gaaccgtgca
     4201 tcgcgttgca gtcgaaccag gtggccgatc cggcggcccc ggtgaactgc cgtatgccgc
     4261 aggcggttgc gaaccgggtg agcgcctcgg gggtgggcgt tccggcgtcc tgcatctgca
     4321 gcgacttctt gtagttgtcc ttcggggtcg cgcccgcgca gccgaggaac gtgcggtgcg
     4381 aacccggcat gatcatcagg ctgccgttgg tcgtgtggtt cggcgtgagg gcgatggaca
     4441 ccgataccgt ccgcatgcgg ggcaggccgt cctcggcgtg ccaggtctcg aagtcggagt
     4501 gccagtagaa gccgctggcg ccgaagccgg gcttggcgtt gatgcgggac tggtggacgt
     4561 acacgtcgga gccgaggatc tgccgggcgc ggtccgcgat ccgcggatcg gcggcgaggc
     4621 gcgcgaagac ctcgctgatg cggtggacct cgaagaccga gcggacctcg cccgatcccg
     4681 gttccacgat ggcccgttcg tcggcccgca cggcggggtc gctcaccagc cggtccatct
     4741 cggcccggta ctcctcgacc tcttccgggc cgatcagctg ctcggcggca aggaagccgt
     4801 cgcgttcgaa gtcctccagt tcgcgcgccc agaacggtcc gggggtgccg ggttcggacc
     4861 acacgacggg gtccttgcgg ccgatgagga cttcttcgga gccccgggtc gggtacaggt
     4921 cggcgagggt gcgttcggag acagtggtca tggtggtgct tgcctctcct ctcggggtcg
     4981 gtgggttcct gcggggtggg tcagaccggg tccggctcgg tgagcagcgg gtacacgccg
     5041 ttctcgtcgt ggtcctcgcg gccggtgacc ggcgggttga agacgcacag gcagcggaac
     5101 tcgcggacga cgcgcagggt gtggcgctcg tggccgtcga ggacgtagac ggtgccgggc
     5161 gtgatggtgt gcttctcgcc ggtctcctcg tcggtgagtt cggcctcgcc ctcgacgcac
     5221 acgaccgcct cgacgtggtt cgcgtaccac atggacgtct cggtgccggc gtacaggacg
     5281 gtttcgtgga gggagaagcc gacgcgttcc ttcgccagga tgaggcgctt gctctcccag
     5341 gtgcccgacg ccgccctgac gtggcggtcg gtgccttcga tgtcctggaa ggatcggacg
     5401 atcatggtgg tgcggccttt cgtggtgacg gcggaggggg tggtgggtgc ggcgggtcag
     5461 gccgtctcgc ggacggaccg cgcgaggatg tcgaggccct cgtcgagctc gtccgccgat
     5521 acggtgagcg gcggcagcag cttgaccacc tcgctctccg gcccggaggt ctcgacgagc
     5581 aggccgtgct cgaacgcccg gtggcagacc tcggcggcgc gctccttgcg ggtgaactcg
     5641 aggccccaca ccatgccgcg gccgcggtac gaggcgccgt cccgctcgtg gtccgcgcag
     5701 aggtcgacga gcgcctgctc gatgcgggcg ccctggacac gggtgcgttc gcgcaggacg
     5761 ccgtcgcgcc agtaggtctg cagtgcggcg gtcgccgtga cgaaggcggg gttgttgccg
     5821 cggaacgtgc cgttgtgctc accgggctcc cagatgtcga gctcgggctt gaacaggcag
     5881 agcgacatgg gcagtccgta gccgctgatg gacttggaga cggtgacgat gtcgggtgtg
     5941 atgcccgctt cctcgaagga gaagaagtcg ccggtccgtc cgcagcccat ctgcacgtcg
     6001 tcgacgatca gcagcatgtc ccggcggcgg cacaggtcgg cgagggcacg cagccactcc
     6061 gcgcgcgcca cgttgacgcc gccctcgccc tgtacggtct cgacgatcac ggcggccggg
     6121 tcgttgaggc cggatccgct gtcctccagg agccgctcga accagaggaa gtccgggacg
     6181 cggccgccga ggtagttgtc gaagggcatg ggcgtgctgt ggaccagcgg tacgccggcg
     6241 ccggcgcgct tgaaggcgtt gccggtgacg gccagcgagc ccagggacat gccgtggaag
     6301 gcgttggtga aggagacgat cgcctcgcgc cccttggcct tgcgcgccag cttgagcgcc
     6361 gcctcgacgg cgttggtgcc ggtggggccc gggaacatca ccttgtacgg cagtccgcgg
     6421 ggggcgagga cggtggagtg gaaggtctcc aggaaggccc gcttggcggt cgtcgacatg
     6481 tccagggcgt gcgtgatgcc gtcgcgcgcg atgtagtcga gcagggcctg tttgaggacg
     6541 gggttgttgt gcccgtagtt gagggagccg gcgcccgcga agaagtccag gtaggagcgg
     6601 ccgttctcgt cgtggaggcg gctgccctcc gcgcggtcga agacgaccgg ccaggcgcgg
     6661 cagtagctgc gtacctccga ctcgacggtg tcgaagacgg tcgggatcgg tgctgtcgtg
     6721 gtcaaggggg acctgcctct cctgcggtgt ccgccggcgg acggtggcgg acggtgatgg
     6781 acgtggtggt cggcggccgg acgcggtggt cggcggccgg gggtgggggc cggtggccgg
     6841 tggccggggt ggtggccggt gtcagagggc caggggcgcg atgcggtgca gcacctcggc
     6901 gtcgtggtgt ccggcgccgc cggggaagtg ctcctgcgcg aagaggacct cccgggcgag
     6961 ttgggcaccg tggcgttcgg cgaaggacgc gaacagccgt tccgaagcgg tgttccccgg
     7021 gctgatggtg gtctcgagcg cgtcgagcgg gtgctcggcg gcgacccgtg cggtgaggtg
     7081 gtcgagaagg tgcccggcga tccccagccc gcgggcgtcg gcgtcgacgg cgacctgcca
     7141 gatcagcagg gtggcggggg tgtcggggcg caggtacccg gtgatgaagc cgaggggccg
     7201 gccgtcgccg tcgcgggcga cggcggaggt ggcggcgaag tcacggcacc agagcaggta
     7261 gctgtagggg gagttgacgt ccagcgtctt cgcgtcgcgg gcgatgcgcc agaggtcggg
     7321 gccgtcgcac aggtcggggc ggtcggcccg gattccggag ggactcgtcg tgggggcggg
     7381 ggttcggggg cgggtggcca ggtctgcatg agcaccggtc atgcgggccg aagctaccga
     7441 tcatcggctg aaaagacatg cccgaacggg gttccgtaca cgctgcgtcc gtgttattcg
     7501 gcagcggcgc ccgggtggca ctggcccgtt ctcccccacg aagaaaacag ctatttatcc
     7561 ggacaaccca gcccaaaatt ctggaagata acgagactat aacgtctttg ccctgtgcca
     7621 tatttgcggt gtgaaagcca cgacattcgc gagtcccccc gtttcggctc cggagaacgg
     7681 gaaaggcgag tgggcccttt cggcccgtcg tgtgtcccgg acagccgggc ggtacaaagg
     7741 ggtatgagcg agcaacagcg acccgcggat ccgtcacccg ccgcggacga catcgacgag
     7801 gtgacgcacg cggtcctggc cgcctcccaa cggctcgcgt ccatcgccgc ccgcgccctg
     7861 gcagccgccg gggacggggg ccgcggcggc cacggggtca cgctcgccca gttccggatg
     7921 ctcgaggtcc tggtggcgcg cgggcacgtg aaactggtcg ccctcgcagg gctgatggcg
     7981 gtcaacccgt cgaccgccat gcgcatgatc gaccggctga tcgccgcggg cctggcggac
     8041 cgcaggccca accgggagaa ccggcgggag acggtgctca ccgccacgga ctccggcctg
     8101 cggctggtgg gcgaggtgac ggcccggcgc cgggcggaga cggccgccgc cctcgcccgg
     8161 ctccggcccg ccgaacgcac cgtactgatc ggcgcgctcg gcgcgttcgc cgccacgggc
     8221 ccgcagcccg ccgaggacgg accccacgcc ttcccggacc tcctcgggtg cgcgcagccg
     8281 tacgcccagg gctgaggcgg gccgcgcgcg gctgcggccg gggcgagggg ccggggctac
     8341 ggctggctgt cgagccagct caactcgtgc gcgtagagca cccgctggca gaagtccgcg
     8401 ccctcgggat caccggggcc cgggttcgcc cggcagtcgt cctccgaacc cggacggtgt
     8461 tcggagaggg accacaggcg tcccgtgccg cgctcgacgt agaggtcctc ggcgccccgg
     8521 gcggtcttga cggcctgacc gtcctggacc agccggccgt cactccagag gtagcgccac
     8581 aggttgcccg gctcgtccgt ccccttggac tcgttcacgt acagcgtgcc gttgtacgcc
     8641 gccgcgccct gggcgccgcc attggccagg ggcaggtagt tggcccagcc ctgcgccgtc
     8701 acgtccgcgg agcgttgctc caatgccgcg accgggtacg aggcgatgcg gcccgtggag
     8761 gggtagccgc tctccggacg gcagtactcg cccatgatca ggcggtccgg acccgtactg
     8821 cggtccaggg agatgtagga cgccttcggg gcgccggtgg agacgcaggc gaaggagccc
     8881 ttggggttgc tctgcgtcgc cttgaacttc catgccgcca cctgcggcat gacgtagcgg
     8941 tagccgaagc tgtaccagac gttgtcgtgc cggccgacct tcgtcttgtc ctcgacgtcg
     9001 gcggtggtct tcaccccgtc ggcgtcggtt cccatccggt cgtgcgtggc ggggtcgccg
     9061 tccgggtcga ggtccatgat gaagcgcatg tcgaagacgc gcaggccgcc ccgcgtgtcc
     9121 gcgacgtaga ggtagttgcc gtaccagacg atcccgccgg cgtggacgcc cttctggatc
     9181 gggtactcgg cggcgtgcag gccgtcgaag gagacgtggt ccgcggagtt cacataggtc
     9241 cagcccagca gcacgtgccg gtacttgatc tcggggctgc cggtggccgg gttgttccgg
     9301 ctctgcacga aggtgatccg gacgcccttc tggttgcagg cgtcattggc ctccagctcg
     9361 gcggcggtgc agtcgccggt cgccgggttc gggtccgtct cgcggcccgg gtcccagccg
     9421 tcgtaggagg cgaacagctg gatcttcccg gctgcgcccc agagctcgtt gtccttggcg
     9481 tcggagacgc cggtgaaacc ctgcgggacc cactccctgg agacggagtc gtcgtccttc
     9541 aggcagtact tgacggccgg gtcgggggcg gcggcgaacg ggtcgcggga acaccccggc
     9601 tcgatgggac ggttcgccgc ggccagcagg tcctgtacgc ccgactcggg cagcaccgag
     9661 gccaggcggg cgaccctccg ggtggtctcg gcgttcatct cgcccggcga cagcgtgaaa
     9721 tcgccgggcg aggcggggcc gccgggtgcg gcggcggcgc gggcaccgaa ggccgagggg
     9781 gcgccttcgg tcgcgcgggg ctcctggatc caggaccccg gcacatcggg cggaaggtcc
     9841 ttcgccgtcg ccggtcctgc gagaccggtg aacgcgagcg ccgccgcggc ggcggccgag
     9901 agccaggcgc gacgtattcg acttgagggc atgcgggagt tgctccgttg ctcgtgtgac
     9961 aggtgagtca agcggaggct tccagaacac gtgcacggaa cacaagggtg atcgactcgt
    10021 caatggccgg tccgagcagg ccggttgaag acccccgtca acggtgggga tcgcgccaca
    10081 tgggccacag cgtcggtccg ccgcccggga gccggatctc gccggtgacg cggaagccca
    10141 gccgttcgta gaaggggacg ttggcgggcg cgctggactc caggtaggcg ggttgtccga
    10201 gccggtcgca gtcggccagt tgctggcgca gcagcgccga gccgactccc ctcccctgaa
    10261 gtccccggtc cgtccccacg gagggcaggt accagtgcgg tgtccgcgga tggacctcgt
    10321 gcatggcgtt ctggacctcc ccggcccgcg gcatcccgcg gaggccgaag acacccgcgt
    10381 accggggcag cgcggccagg tcctg
//
