LOCUS       NZ_CP020039            10486 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. 3211 isolate 3 chromosome, complete genome.
ACCESSION   NZ_CP020039
VERSION     NZ_CP020039.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 3211
  ORGANISM  Streptomyces sp. 3211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Complete genomes of three Streptomyces isolated from disease
            suppressive soils
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2017) Biotechnology Institute, University of
            Minnesota, 1479 Gortner Ave, Saint Paul, MN 55108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JAN-2017
            Assembly Method                   :: PacBio v. Jan-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:18:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,254
            CDSs (total)                      :: 8,154
            Genes (coding)                    :: 7,815
            CDSs (with protein)               :: 7,815
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 206 of 340
            Pseudo Genes (incomplete)         :: 257 of 340
            Pseudo Genes (internal stop)      :: 68 of 340
            Pseudo Genes (multiple problems)  :: 153 of 340
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:04:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 490922
            Orig. end                         :: 501408
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Michael Smanski.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10486
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10486
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10486
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5486](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5486)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    730..746
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.91"
                     /tool="antismash"
     gene            920..2476
                     /locus_tag="B4U39_RS02445"
     CDS             920..2476
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B4U39_RS02445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="PKD domain-containing protein"
                     /protein_id="WP_079403515.1"
                     /transl_table=11
                     /translation="MLKTAASAFTFSSPADRSVQQNRPAAGKKAAAAPQASGATAGADL
                     KVGLSGTGTSARAIELKSDITSSMGTPLTVVIDWGDGTASTEHAGGSETKTSHHVYAEV
                     GSYTVKVTVYGVDGANGPSATNEMPFVTPGSEFTPHSPTRLLDSRDGTGAAQAAQVPAL
                     GEARVKVAGNGGIPADVAAVVLNVTVTNTRSAGYVSAYADGTERPTTSNVNYAAGDSVP
                     NLVIVPVGKNGYVNLYNYGERGVDLIADVTGYFTRSSANGYTPTDPTRFVDTREGLGTS
                     RGMVPGRGQFGVQVAGTRGIPAGAKAVALNVTVTNPRTAGYLTVFPGGQSAPNTSNVNF
                     SSGQTIANSVIVPVDADGKIMVRNGSWDPADVVVDVVGYYSPDSQSAYIPVRPVRDLDT
                     RDASQWPSGPLKAGRYLYSTYGEENPTATAMVLNTTVTNTASAGYLTVSPDPNALWQYQ
                     NKTHTPPIPPNSSTLNWTAGKTVPNLVQASSGVNGIVDFFNRSGNNIDLVIDIFGVYDR
                     H"
     gene            complement(2782..4011)
                     /locus_tag="B4U39_RS02450"
     CDS             complement(2782..4011)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554413.1"
                     /locus_tag="B4U39_RS02450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_079403516.1"
                     /transl_table=11
                     /translation="MASLDTARPLSARPALPRVFLVWLAGTRASLFGDAVLYFALGWAA
                     SAHGGGTGALVLTAITLPRTLLLLLGGAVGDRFGARLVMITGDVVMLTVTLVLALVGPD
                     LGASPWLLVLVAVVVGTVDAFYLPATGSMPRRLVGKEQLPRALAMQQAGSQIVSLLGAP
                     VGAVLVAAAGLTGAAAADAVTFAIVLVVLVRVRPAFDVERSPREEGLLAAVADGVRIAL
                     GGPVLRPALLLTAAAAAFLLPVVSLLNPLLAREHGWSAGVAGLIAGGQSLGMIAVALVV
                     SRRGTMYRIGRGAALGLCVSAPGITAVALADTAAVAVAGGIVVGAGSGMFACHIGPLVL
                     TNVPDTHLARMQALLVLVQSLALVVGNNALGALADAQGATLAAAVCAVGACAAGTVGLV
                     LTPVRRLRQE"
     gene            complement(4190..4834)
                     /locus_tag="B4U39_RS02455"
     CDS             complement(4190..4834)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712128.1"
                     /locus_tag="B4U39_RS02455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_079403517.1"
                     /transl_table=11
                     /translation="MVPIAVSLGAGLLLGLVGPVAGKWGNPVCVATGIVFSSGWPWACY
                     AFLVGYSRRPKIESALLASLGLIIGVVTYYIFKDMNPAIPAGMESAPSVPAGMELESGA
                     SSGGQILVWGIAAFVFGAPVGLMGNVARTPGIGGLLFRLLVPFIAFFEATQRLNVEADS
                     QGPVFDMTWNATRVAACVVAVALVAHTVWSWRVRRGRADGRAEAVAPERRL"
     gene            complement(5001..5486)
                     /locus_tag="B4U39_RS02460"
     CDS             complement(5001..5486)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950154.1"
                     /locus_tag="B4U39_RS02460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_079403518.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 5.4e-07,
                     bitscore: 28.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISGRSVAFAIAGAAVAAVGLATPATADASAAASASGATVTVHH
                     RGDGTQPPAELGNPSEWGVVTFKMDAAAASFGAQSQACISPSTGGNWCYGWYAREVSPG
                     VINKYCYSNYYQNTKGHSSTVKLAGSTRRVWAAAGNTSNANLTAGLAYTCSTYCSVD"
     misc_feature    complement(5573..5586)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.55"
                     /tool="antismash"
     gene            5988..6563
                     /locus_tag="B4U39_RS02465"
     CDS             5988..6563
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B4U39_RS02465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_079403519.1"
                     /transl_table=11
                     /translation="MNALLKRVWIGLAATLIVSLAAAVLPDQIQINDDGRIHLGAPAPS
                     PSTAAPAPESTSPPLTRDAVDEVIRTAVSAAGLDPAQGRPIGAAGADSNKTGWMAIREQ
                     TAAQSDMNAICADLERQGWRLNPSGDRPEASRSYKRGGWYLLVSTRGKTQAPSTTSALT
                     DSQTYLTVTGLQLNLSDLPLPEITVRIG"
     misc_feature    6620..6635
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            6659..7324
                     /locus_tag="B4U39_RS02470"
     CDS             6659..7324
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B4U39_RS02470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_079403520.1"
                     /transl_table=11
                     /translation="MAGWHGGWVRGIRRTAVALAAPPLAAALAFGVARQSAYGSLGNWG
                     FFLGGVLLPLVLLGWHAAVAARREASLIALSALPAALGAILALASVGHSTLEDRGVEIS
                     CVVLKVTERTETETSMDAEGHWTSTSRTSYDHRLDCPAGGPEYLNTPSQLAKEGEPLAV
                     VHDPQGKVSPRAARDVHGRGMRTAAAVAVGTAVLLGLAGGIREAYEKRRRRPRRPTGR"
     gene            complement(7847..8551)
                     /locus_tag="B4U39_RS02475"
     CDS             complement(7847..8551)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006344589.1"
                     /locus_tag="B4U39_RS02475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1345 domain-containing protein"
                     /protein_id="WP_079403521.1"
                     /transl_table=11
                     /translation="MTEQDPAETDRTSAREPGPTPGVHTESRWPMAAAVVASAVLTLLL
                     PDDLRLGPRWALPVAEGLLLVALISGDPGRISRRSTVLRSVAIALVVVLVGSAIWSTVQ
                     LIDDLIHGGSETNSANALLLAGGSVWASTVLAFSLLYFELDSGGPAARAHHMPPTPALA
                     FPQQLSPELNAPHWRPHYIDYLYLGFTNSTALSPTDVMPLAPWAKSVMTLQSILSLMIL
                     GLVVARAVNVLA"
     gene            complement(8605..8736)
                     /locus_tag="B4U39_RS45285"
     CDS             complement(8605..8736)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B4U39_RS45285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_257789844.1"
                     /transl_table=11
                     /translation="MRAGPGPAVEPAGATGPYAFTCEERHMNPKSRVTGTVRAADAA"
     misc_feature    8786..8801
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     misc_feature    9043..9057
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.78"
                     /tool="antismash"
     misc_feature    9049..9062
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.18"
                     /tool="antismash"
     misc_feature    9112..9121
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.85"
                     /tool="antismash"
     misc_feature    9711..9720
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     misc_feature    10461..10477
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.23"
                     /tool="antismash"
ORIGIN
        1 catactgctt tccggccggc gattcgaacc gccgaccggt ggccccctga ggatccgggg
       61 cggctggatg gtcgtcctgc cgtgggcggc actcctgccg gcggcactgc tcatgccgct
      121 gcaatacctg atcatgatgg gcgcccagtt tgcctacgtg atctgggtcc tgccgagcga
      181 ggactcccgc gacacggcga acatcctgcg catgctcgcc gatccggccg tcccggcgga
      241 acagcgggcc aggattgccc aagcggtcgc cgaactccgg accaggccgg tcgtcgtagc
      301 gctggtagcc gctgcgcagg acgaggaacc ggaagtcgcc gaagcggcac tgacaagcct
      361 ctgcgccata tggcagcacg acggcgtcgt cggcgaagac ctactgctga agctgacgga
      421 ccaggaacag gaccgtgtac gggcactggg cgtcaaggtc cgcagcccct ggtaggcagc
      481 aggtccctca gcaagcggta acccgagcct ggtctcccga ccccggtagg gccgggccct
      541 tcagggacga tgcgagcagc cattgacaca gtcccacacg tacgccgaca ccgaatccgc
      601 gtacctcggc gtgcagcacg ggtttgtgcg gccggcgcgc ggggtgcggg cggcgacgca
      661 ccgttgacac cagggcagtt cacccggtcg cgccgcccta agaccccctc ctcgccgagc
      721 ggcgcagttc tgtaatgtga tcacttccta gagatcaact cgagtgttgg acaacataat
      781 tggggggccg gtgcgcagtc gccgacttcg tttttccgct gccgtgatcg cagcaggcgt
      841 cgggatcatg ccggggggca tggccgaggc cgcgggaccg tccgctgacg gtgcccatgg
      901 catcacttcg tccaaggatg tgctcaagac cgccgcgagc gctttcacct tcagcagccc
      961 ggccgaccgg tcggtgcagc agaaccggcc ggccgcgggg aagaaggccg cggcagctcc
     1021 gcaggcctcg ggcgccacgg cgggcgccga tctcaaggtc ggtctcagcg gtacgggtac
     1081 cagtgcccgg gcgatcgagc tgaagtccga tatcaccagc agcatgggta cgccgctcac
     1141 ggtcgtgatc gactggggcg acggcacggc cagtaccgaa cacgccggtg gttccgagac
     1201 caagacctcc caccacgtgt atgccgaggt gggcagctac acggtcaagg tcaccgtgta
     1261 cggcgtcgac ggcgcgaacg gcccctcggc gaccaacgag atgccgttcg tcaccccggg
     1321 ttccgagttc accccgcaca gccctacccg tctgctcgac agccgggacg gcacgggcgc
     1381 cgcgcaggcc gcccaggtgc cggcgttggg tgaggcccgc gtgaaggtcg ccgggaacgg
     1441 cggcatcccg gccgatgttg ccgccgtggt cctcaacgtc acggtcacca acaccaggag
     1501 cgccggttac gtcagcgcct acgccgacgg caccgagcgt ccgaccacgt cgaacgtgaa
     1561 ctacgcggcc ggcgactcgg tgccgaatct ggtcatcgtg ccggtcggca agaacggcta
     1621 cgtgaacctg tacaactacg gcgaacgggg cgtggacctg atcgccgacg tgaccggcta
     1681 cttcacccgg tcctcggcca acggctacac gccgacggac ccgacgcgtt tcgtcgacac
     1741 ccgcgaaggc ctcggcacgt ccaggggcat ggtccccgga cgcggccagt tcggcgtcca
     1801 ggtcgccggt acccgcggta tcccggccgg cgccaaggcg gtggcgctca acgtgaccgt
     1861 caccaacccg cggacggccg gctacctgac cgtcttcccc ggcgggcaga gcgcgccgaa
     1921 cacgtccaac gtgaacttca gcagtggcca gaccatcgcc aactcggtga tcgttccggt
     1981 cgacgcggac ggcaagatca tggtccgcaa cggcagctgg gatccggccg acgtcgtcgt
     2041 cgacgtcgtc gggtactaca gcccggacag tcagagcgcc tacatcccgg tccgccccgt
     2101 ccgggacctc gacacccgcg acgcctcgca gtggccctcc ggcccgctga aggccggccg
     2161 ctacctctac tcgacgtacg gggaggaaaa ccccacggcc accgccatgg tcctgaacac
     2221 tacggtcacc aacaccgcca gtgccggata cctgacggtc tcccccgacc ccaacgccct
     2281 ttggcagtac cagaacaaga cgcacacccc gccgatcccg ccgaactcct ccaccctgaa
     2341 ctggacggcc ggcaagaccg tgccgaacct ggtccaggcg agcagcggcg tcaacggcat
     2401 cgtcgacttc ttcaaccgca gcggcaacaa catcgacctc gtcatcgaca tcttcggcgt
     2461 ctacgacagg cactgatgcg tccccgccgg cccacgccac acctcccgag gaccacggct
     2521 gtcgtcgccg ccatcggatt cccgaaggcc ctgtgaggcg aaggcggttg gcctggcccg
     2581 aacgcgacgc gtggggccct caacctgcgt gcttcaggcg ccgccagggt ccgggccacc
     2641 ctggcggcgc gcccacgccg gtgcggcccg gcgtcccggc cgggcgggcg tgggaaggca
     2701 tccgccgggg gctgggccac ctgcacccgg cggtgtcagc caccgctggc acaccgcgca
     2761 ccggattggc ctgcccccac ttcattcctg acgcaggcga cggaccggcg tcaggacgag
     2821 acccacggtc cctgcggcgc aggcacccac cgcgcacacc gccgcggcga gcgtggcccc
     2881 ctgggcgtcg gctagtgcgc cgagcgcgtt gttccccacc accagcgcga gactctgaac
     2941 gagcaccagc aacgcctgca tcctggccag atgggtgtcc gggacattgg tcaggaccag
     3001 cggaccgatg tggcaggcga acatcccgga acccgcaccg acgacgattc ctccggccac
     3061 ggccacggcg gccgtatcgg ccagggccac cgcggtgatt cccggcgccg acacacaaag
     3121 ccccagtgcc gcacccctgc cgatccggta catggtgccc cgccgcgaga ccaccagcgc
     3181 cactgcgatc atccccaggc tctggccacc ggcgatcaga ccggccaccc ccgcactcca
     3241 gccgtgctcc cgcgccagga gcgggttcag cagggacacc accggcagga ggaaggcagc
     3301 ggcagcggcc gtgagcagca gcgcgggccg caggaccggg ccccccaggg cgatccgcac
     3361 cccgtcggcc accgccgcca ggaggccctc ctcgcgaggg gaccgttcga cgtcgaaggc
     3421 cgggcggacg cggacgagca ccacgaggac gatcgcgaag gtgaccgcat cggctgctgc
     3481 cgcgccggtc agccctgcgg ccgcgacgag tacggcgccc accggagccc cgagcagcga
     3541 gacgatctgg ctgccggcct gttgcatggc caacgcccgg gggagctgtt ccttccccac
     3601 caggcggcgg ggcatggacc cggtggccgg cagatagaac gcgtcaaccg tgccgacgac
     3661 gacggcgacc aggacgagga gccagggcga tgctcccaag tcgggtccca ccagggccag
     3721 tacgagagtg accgtgagca tcacgacgtc gccggtgatc atcacaaggc gcgcgccgaa
     3781 ccggtcaccg acggcaccac ccagcaacag cagcagggtg cgtggaagcg tgatggcggt
     3841 caggaccaga gcgcccgtcc caccaccatg ggcactggcc gcccagccga gcgcgaagta
     3901 caggacggca tcaccgaaca gcgaggcacg ggtgccggcg agccagacga gaaagacgcg
     3961 aggaagggcc gggcgcgctg agaggggccg ggcggtgtca agggatgcca tgggcgctga
     4021 cgctaaggcc ctcgagcagg tcgagggcaa gcgtccgcct gagccgggcc ggtccgcggc
     4081 gccgtgcaga gccggtggtg tcgaagccga agctcagccg gtcgaccggg cctcttcgga
     4141 gggcggggct ttgcattgtt gcagtccagc tgccccggca gaacaagggt cagagacgac
     4201 gttcgggcgc gacagcctcc gctcgcccgt cggctcggcc acgccgaaca cgccagctcc
     4261 acacggtgtg tgccacgaga gccacggcga cgacgcaagc tgccacgcga gtcgcgttcc
     4321 aggtcatgtc gaaaaccggc ccctgcgagt ccgcctctac gttcaatcgc tgggtcgcct
     4381 cgaagaaggc gatgaacggg acaagcagac ggaacagtag gccaccgatt ccgggcgtgc
     4441 gcgctacgtt ccccatgagt cccacaggcg caccgaacac aaaagcagct attccccaca
     4501 cgagaatctg ccccccgctg gaggcgccgg attccaactc cattccagcc ggaacagagg
     4561 gggcggattc cattcctgcc ggaatggcgg gattcatatc cttgaaaatg tagtaggtga
     4621 ccacgccgat gatcaaaccc aaagacgcca gcaatgccga ctcaattttg ggtcggcggg
     4681 aatagcccac cagaaatgcg tagcaggccc acggccaacc actggaaaag acaatgcccg
     4741 tggcgacgca aacgggattg ccccatttcc cggcaacggg gccaaccaga ccgagcagca
     4801 aacccgctcc gagagacaca gcgatcggca ccatgtgagc acgtgtgaag cgtgtgattg
     4861 ttctaccccc gttcagtggg tgagtccgag ttggaggtgg caccactccg gggctgcacg
     4921 ggcgaccgag cagccccgga gtgactcatc aacctaccgc accttctgag gagttactga
     4981 ttggtgcggg cgaccagaac ctagtcgacg ctgcagtagg tggagcaggt gtaggcgaga
     5041 cccgcagtga ggttcgcgtt agaggtgttc ccggccgcgg cccacacgcg acgcgtactg
     5101 cccgcgagct tcaccgtgga cgagtgcccc ttggtgttct ggtagtaatt cgagtagcag
     5161 tacttgttga tcaccccggg gctgacctct cgcgcgtacc agccgtaaca ccagttgcca
     5221 ccggtggacg ggctgatgca cgcctgcgac tgcgcgccga aagaggctgc agcagcatcc
     5281 atcttgaatg tcaccacacc ccattcggag gggttgccca gctctgcagg cggctgagtg
     5341 ccgtccccgc gatggtggac ggtgacggtg gctccggagg cgctggcggc tgcagacgcg
     5401 tccgcggtgg ccggcgtcgc taggccgaca gctgcaacgg ctgcgcccgc gatggcgaaa
     5461 gcgacggacc tgccggaaat cttcacgagt ttcctccctg attccccctg ttgggaacga
     5521 gagagatcct tcacgggtcc gcacttgcca ctgaagtgtc ggactgggca acttttttcg
     5581 actaatcatc ctaggtcagg cgataggaaa tctaggaata tccaaacgat gccacatgaa
     5641 tcgggcacgg cggttttcgg tcaacggcgc tatttccctt tacccattca tcgccttcag
     5701 tgttgatcga tggctgatat ctaccgcttc tcttggccaa tggcgtcggt gtgcccaccc
     5761 atcgcgttcg cgcgatcccg gacgcaaacg accgccgtgc agagcccgcc gtgaagagac
     5821 cgcccctgcc cccaccgtgc ccggacaggt cggaccaccg gccgcacgcg gccggcaccg
     5881 tccccgccgg cacgacggcg ccggcgcccc cgctcgctca gggctgccgg gcgccgagca
     5941 gtatgtgtga gactccctct gaatgatcac tcggggggag tttcgcgttg aacgcgctgt
     6001 tgaagagggt gtggatcggg ctggccgcga ccttgatcgt gagtctggcc gccgcggtcc
     6061 tgccggacca gatccagata aacgacgacg ggcgcatcca cctgggcgcg cccgcgccgt
     6121 cgccgtcaac ggcggcgccc gcaccggagt ccacgtcacc gcccctcaca cgggacgccg
     6181 tggacgaggt gatccggacc gcggtcagcg cggcgggcct cgacccggcg cagggccgcc
     6241 cgatcggcgc ggccggtgcc gactcgaaca agacgggctg gatggccatc cgcgagcaga
     6301 ccgccgcgca gtcggacatg aacgcgatct gcgccgacct ggagcgccag ggctggaggc
     6361 tgaacccgag tggcgaccgc cccgaagcct cccgcagcta caagcgcggg ggctggtacc
     6421 tgctggtgag cacgcgcggg aagacccagg ctccgtccac cacctccgcc ttgaccgact
     6481 ctcagaccta cctgaccgtg accgggctcc agctgaacct ttccgaccta cccctccccg
     6541 agatcaccgt ccgcatcggc tgacccctca ctccgggccg gacgatgtcc agggctcttt
     6601 acgtgcccgt gccgtcgcgg ttacctggag gtcacaggta cgagccacgg gggcaggcat
     6661 ggcgggctgg cacggggggt gggtacgcgg gatccggcgg accgcagtgg cgttggccgc
     6721 gccgccgctg gccgcggccc tggcgttcgg ggtggcacgg cagtcggcgt acgggtcgct
     6781 gggcaactgg gggttcttcc tcggcggggt gctgctcccg ctggtcctgc tcggctggca
     6841 cgccgccgtc gccgcgcggc gcgaggcctc gctcatcgcg ctgtccgccc tcccggccgc
     6901 cctcggcgcc atcctggcac tcgcctccgt ggggcacagc acgctggagg accggggcgt
     6961 cgagatcagc tgcgtcgtgc tgaaggtcac cgagcgcacc gagacggaaa cctccatgga
     7021 cgcagagggc cactggacct ccacgagccg gacctcgtac gaccaccgcc tggactgccc
     7081 cgcgggcggc ccggagtacc tcaacacccc ctcgcagctc gcgaaggagg gcgagcccct
     7141 ggccgtggtg cacgacccgc agggcaaggt ctccccgcgg gccgccaggg acgtccacgg
     7201 ccggggcatg cgcaccgcag ccgcggtggc ggtcgggacg gcggtgcttc tggggctggc
     7261 cggcgggatc cgggaggcgt acgagaagcg gcgccgcagg cctcggcggc cgacagggcg
     7321 gtgaagcccc gcccgcggac ggacgcccgc cccgagctcc cccgcaccca cccacaggcg
     7381 cgaccagcac ctcccccgtg cacggcaacg acctccacgg cgtacgggtg gagtcgcgtt
     7441 cttcggcccc gaggagccct gagtcccggc cgcccccgca ccggccgctc gcgcacggct
     7501 cgaccgggca cggaaccggc tggccgccac cttccgcggc acgaccgccc gagccgacga
     7561 ggtccggtgc gtgccactgg ggcagcccgg aggagctcgc cctgctgaag ctccccgaca
     7621 cggaagccga cgccgatctt cgcccgcgaa ctgaccggcg gccgcggcgc ccacgcgtac
     7681 gacgtcggca acatcggcgc ctccttccac cgctcggcgg tcgcggttcg tccggccgga
     7741 agaggcggcg gaaggcgtac ggaccgaact ggccgcctgg ctcgtgcgcg cggcccccgc
     7801 acgcaccgcg ccaccgctac gcctgacgca gggccgccgg gccgtttcag gccaggacgt
     7861 tcacggccct ggcaacgacg agtcccagga tcatgagcga aaggatggac tggagggtca
     7921 tgacgctctt ggcccagggg gcgagcggca tgacgtcggt ggggctcagc gcggtggagt
     7981 tggtgaaccc gaggtagagg tagtcgatgt agtgggggcg ccagtgcggg gcgttcagtt
     8041 cggggctgag ctgttgcgga aaggcgagcg ccggagtggg gggcatgtgg tgggcccggg
     8101 cggccggccc gccgctgtcg agttcgaagt acaggaggga gaaggcgagg acggtggagg
     8161 cccagacgct gccgccggcc agcaggaggg cattcgccga gttggtctcg ctcccgccgt
     8221 ggatcagatc gtcgatcagc tggaccgtcg accagatggc gctgcccacg agcacgacga
     8281 ccagggcgat cgccactgag cgcaggacgg tggagcggcg gctgatccga ccggggtctc
     8341 ccgagatcag ggccaccagc agaaggccct cggcgacggg aagcgcccag cggggtccga
     8401 ggcggaggtc gtcggggagc agcagggtga gtaccgccga ggcgacgacc gctgcggcca
     8461 tcggccagcg gctctcggtg tggacacctg gtgtcggccc cggctcgcgc gcgctcgtgc
     8521 ggtccgtttc ggcggggtcc tgttcggtca cggccgatcg cttccccggt ccggtggcag
     8581 gtcagatcgc atgtgccgtc ggactcatgc cgcgtcggcg gcgcggacgg tgccggtcac
     8641 ccttgatttc gggttcatgt gccgctcctc gcaggtgaag gcgtacgggc cggttgcccc
     8701 ggcgggctcg acggccgggc ccgggccggc gcgcatcgac ctgagcgtgc tcgacgaccg
     8761 atccacgacc gatccacacc ttcagggtga tccacctcac actccgccac aactccaagc
     8821 cgcatggcgc gctgtcgacg aatacgtgag ggcggggcgg gacagcggga tcgggcgccg
     8881 tgtcgtgttc ctcgggtgaa ctcctgcgcc tcgcgcgggc ttcgacggcc ggtcggagca
     8941 atacgtggcc ctcgcacctc acgggtcgtg accgaaggaa ttcggctggt agcaattagg
     9001 tgaattcctg actgaattcc cgtcaataag cctccgcttc atatctcgat ttgataacaa
     9061 cccaccctcg aaggactgga ccattgcggg gcgacgcgcg taactggtct ttatttattg
     9121 agagttgatt gttcgtcaaa tcaggccgat aggtaacgga atgatttctc ggggcgcgcc
     9181 cccgtttgtc cgaattctcc tccgcattcg tctggcaggc ttccgcgcac ccagtcttcc
     9241 gacaacgatt tgaggtgccc atgacaggac gtcgcagacg gcctgccgcc gccgaccacc
     9301 gccgcaaacc ccggcggttg atactcgtca ccgccgccac cgcagcggcg gggctgatag
     9361 ccgcaggcat cgcctattcc ggaatcggcg acggcgccca ggcggcggcg cccgtacagg
     9421 ccgccgccga ggcaccggcc gccgcgcccg cgcgcgacca ggagcccgag ccgatcgcgg
     9481 ccgcgccctc caacgagacc gcgcgcggca tgatcttcga cgggctggag gccgcgccca
     9541 agggcgacaa gtgcgtgggt gtctaccgca ccaccgccgg gctgtgcacg cacggccccg
     9601 acgccccgcc caagggcgtc gacatcacga aggacatcga gccggccgtc aaggagactg
     9661 ctccggcggc cgatccggcc agccccgcgg ccgccgaccc ggccagcaag gaaggcggcg
     9721 ggcgtcctca ggacgcgccc gcggccgacg cctcggccgc caaggcctcc gcgcccgaac
     9781 cggcggccgc ggccggcagc cagaacgtcg ccgccggccc cgcgggccag accgtccagt
     9841 gcgacggcga cggcagcacc ggcaaccgcg tacaggtcgt ctacgtccac gcgccgggca
     9901 aggaccgcta ctccgagtac gtcgcctcgt tccgcaagtg ggccgccgac gcggacctca
     9961 tctactcggc gagtgccaag gagaccggcg gagtacgcca catccgctat gtgacggccg
    10021 ccgactgcac cccgaccgtg ctcaacatcg agctcccggc ctcggcgctg gccgagttca
    10081 gcgcgaccaa caacgcgctc gccggcaagg gcctcgaccg tcgcgaccgc aagtacatga
    10141 tcttcgctga cacccaggtc tactgcggca tcggcacgtt cgcgggcgac gagcggcccg
    10201 gccaggccaa ccagagcaac ttcggcccct cctacgggcg tacggactcc ggctgctggg
    10261 gcggtcacac cgccgcgcac gaactcggcc acaacctggg cgcggtcaac aacagcgccc
    10321 cgaacaccag ccgtggcgcg cactgcaccg acgagtacga cgtgatgtgc tactcggaca
    10381 ccccgtacta cccgcagatg cgcaacatct gcaccaaccc ggcggccgag aacatcctgg
    10441 actgcaacca cgacgactac ttccacacca gtcccaagcc cggcag
//
