LOCUS       NZ_CP020039            10960 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. 3211 isolate 3 chromosome, complete genome.
ACCESSION   NZ_CP020039
VERSION     NZ_CP020039.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 3211
  ORGANISM  Streptomyces sp. 3211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Complete genomes of three Streptomyces isolated from disease
            suppressive soils
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8232231)
  AUTHORS   Heinsch,S.C., Hsu,S., Hansen,L., Kinkel,L. and Smanski,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2017) Biotechnology Institute, University of
            Minnesota, 1479 Gortner Ave, Saint Paul, MN 55108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JAN-2017
            Assembly Method                   :: PacBio v. Jan-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:18:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,254
            CDSs (total)                      :: 8,154
            Genes (coding)                    :: 7,815
            CDSs (with protein)               :: 7,815
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 206 of 340
            Pseudo Genes (incomplete)         :: 257 of 340
            Pseudo Genes (internal stop)      :: 68 of 340
            Pseudo Genes (multiple problems)  :: 153 of 340
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:04:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 136220
            Orig. end                         :: 147180
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Michael Smanski.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10960
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="3"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10960
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10960
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5960](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5960
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(137..1033)
                     /locus_tag="B4U39_RS00675"
     CDS             complement(137..1033)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330964.1"
                     /locus_tag="B4U39_RS00675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_079403276.1"
                     /transl_table=11
                     /translation="MDIDVLGALAVRENGISITPTAPKPRQVLALLALHADQVVPVSAL
                     IEELWAGEPPRSARTTLQTYVLQLRALIAGALERGTATGPGPGAGDDDTPTGTAAGTGA
                     GRVVRTAKDILVTLPGGYLLSSGGGTLDVREFDRLAGTGYRAMDAGDFPGAARLLREAL
                     ALWSGPAFADVQGGIQLDMETRRLEETRLCALDQRIEADLRLGRHRELLAELTVLVTRY
                     RTHENLHGQFMLALHRSGRRGEALDVYQRLRATLVRELGLEPSIALRRLQRSILMAGPE
                     SPAEAHAEPGGERLVRV"
     misc_feature    complement(827..829)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(1060..1075)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.92"
                     /tool="antismash"
     misc_feature    1092..1106
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.18"
                     /tool="antismash"
     gene            complement(1273..2217)
                     /locus_tag="B4U39_RS45635"
     CDS             complement(1273..2217)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="B4U39_RS45635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_335755580.1"
                     /transl_table=11
                     /translation="MVKQERAARTREALVRAAAEVFAEEGFTAASIAVISRRARVSAGG
                     LHFHFAGKTVLARAVQDRAAQALSRITAGPAVPPPTATTPTTTIPAPTTPAPAPAPDPG
                     PASTPTTPEPGPASAPGPAPAPTTPAPVPASAATTPASVPGPDGSAGTGTGTGTGADPG
                     GGGGAGAGAGSGGGSLQVLVDSTHRLMALLAHDAVVRAGFALCADPSFEGTADLRRQWR
                     LWVEAAVAAAAREGLLAEGLLPEDAARGVVAATVGLEVLGAYDRQWLAPHTLTRFWTLM
                     LPRLASPPALDALLPQGSPGSAAPPPDPDTESD"
     gene            complement(2392..3072)
                     /locus_tag="B4U39_RS00685"
     CDS             complement(2392..3072)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676872.1"
                     /locus_tag="B4U39_RS00685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_030390789.1"
                     /transl_table=11
                     /translation="MARQERAVRTRRAILEAAAAVFDERGYDAATIADILARAGVTKGA
                     LYFHFSSKQELAQGVLDEQFAEGGVPHRESKLQELVDVAMVLAYRMKREPMLSAGARLS
                     LGPGMRDIFGGGSVPGWIEFTRTLLVRAKEQGELLPHVDPAETAWILSACWTGAQIYSQ
                     TLSDRSDIEQRVAAVYQHICPSIATPAMLARLDIAGDRGERVSAEMRRGPGRGAAQESE
                     PALS"
     gene            complement(3193..4512)
                     /locus_tag="B4U39_RS00690"
     CDS             complement(3193..4512)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004948158.1"
                     /locus_tag="B4U39_RS00690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_079403277.1"
                     /sec_met_domain="p450 (E-value: 1.2e-13, bitscore: 49.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRQCPFAIDALGGDVHAEAARVREQGPATRVLLPGGVSAWAVGR
                     SADMRRLLVDPRVSKDAYRHWPAWISGEVGEQWPLAIWVSVQNMITAYGSEHARLRRPA
                     AAAFTARRAAGLRPRIEEVVGELLEDLAASAPGGAVDLRTRFAHELPTRIVFELFGIPE
                     HSWGPLHKIINAFFDTGITAEEAQRNAADLDVVMADLVAHRRRHPGDDMTSALIAARDT
                     ATDPRTATDARTATAAADVGAGGGVEGAGLSEKELVDSLILLFTAGYETSVNLICTALH
                     ELLRNPAQLALVRAGLASWEDVVEEALRIEPPAFYGLLRFAVEDIDMGEGVVIGQGDPI
                     LISFGSAGRDPEVHGADADRFDVLRATRGQHLSFGFGTHHCLGATLARLECAVAVRRFF
                     ETFPAASLAGVGELGRVESFISNGLRELPVVLGPRAPVAD"
     misc_feature    4648..4663
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.25"
                     /tool="antismash"
     misc_feature    4977..4992
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.07"
                     /tool="antismash"
     gene            5001..5960
                     /locus_tag="B4U39_RS00695"
     CDS             5001..5960
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106284.1"
                     /locus_tag="B4U39_RS00695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_237296535.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-20, bitscore: 72.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMLTIGKHAPVLPLPGTEQPWMRCNDARQLTTTVPREYVHRSSL
                     AEVFLTGCERTGQDTFTLTGQWPRAHTFFTSADGTGHDPVQAGETVRQVGLYLCHAVYG
                     VPLGHNVLLWSLDFTTRPEHLRIGRGPSDLTMNALCTDFGWKGNRFSGYLSVDIHRNGE
                     LAARGTARFSCVAPATYRRLRGDRGRLALTRPLPRRTPVPAHTTGRLLPFDVVLAPTDH
                     PGRWQLNPDLDHPILFEHANDHHPGMVLVEAARQAASALHTPTTFTPAAIATEFHHYAE
                     LDAPCWIDATLTTPGHVTITGHQENRTIFHSTVTATTT"
     misc_feature    complement(6543..6557)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     misc_feature    complement(6650..6663)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.39"
                     /tool="antismash"
     misc_feature    complement(6839..6853)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     misc_feature    7566..7577
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 16.74"
                     /tool="antismash"
     gene            7890..8294
                     /locus_tag="B4U39_RS00700"
     CDS             7890..8294
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124490.1"
                     /locus_tag="B4U39_RS00700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_079403278.1"
                     /transl_table=11
                     /translation="MRIGELARRTGVSPRSLRYYEDQGLLTSSRSDAGQRHYADAAVQR
                     VTLIRQLFDAGLSSRVIATVLPCVDIPGDPGVAEETFTAMMRERDRIDADIAHLIETRT
                     ALDALIRANSRHRAQLSTAPRTGDAADLVL"
     gene            8394..9317
                     /locus_tag="B4U39_RS00705"
     CDS             8394..9317
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (21-225). E-value: 3.2e-29.
                     Score: 94.0. Matches aSDomain:
                     nrpspksdomains_B4U39_RS00705_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124491.1"
                     /locus_tag="B4U39_RS00705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_107469887.1"
                     /sec_met_domain="ADH_N (E-value: 2.3e-08, bitscore: 32.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGQAWGFDRHGGPEVQAFFERPDPVPGRGEVLIRVDVAGVNPLDH
                     LLRSGPVDGLEDGRPFPRVLGMEAAGTVLARGQDVTGLEVGDAVFGFALTGGGTYARTT
                     VLSAANTARIPAGLSPTVAATLPVAGTTAVDVLDQLGLPAGATVLVNGVGGGVGLAVAR
                     LAIGRRLHVIGTGSTAKREHAESLGALFIDYTTQDVAAAARQLVPDGFDAIVDLVAGTS
                     LQAVAPLARDPRNIIAVSDTSVAGLGGRFVERRLDRENLERSARLALDGLLAPAITAVH
                     PLSEAPAALAAVENGHTTGKTVIKVT"
     aSDomain        8457..9068
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4U39_RS00705_PKS_ER.1"
                     /evalue="3.20E-29"
                     /label="B4U39_RS00705_PKS_ER.1"
                     /locus_tag="B4U39_RS00705"
                     /protein_end="225"
                     /protein_start="21"
                     /score="94.0"
                     /tool="antismash"
                     /translation="PDPVPGRGEVLIRVDVAGVNPLDHLLRSGPVDGLEDGRPFPRVLG
                     MEAAGTVLARGQDVTGLEVGDAVFGFALTGGGTYARTTVLSAANTARIPAGLSPTVAAT
                     LPVAGTTAVDVLDQLGLPAGATVLVNGVGGGVGLAVARLAIGRRLHVIGTGSTAKREHA
                     ESLGALFIDYTTQDVAAAARQLVPDGFDAIVDLVAGTSLQA"
     CDS_motif       8826..8888
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4U39_RS00705_0001"
                     /evalue="4.00E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="B4U39_RS00705"
                     /protein_end="165"
                     /protein_start="144"
                     /score="10.0"
                     /tool="antismash"
                     /translation="GATVLVNGVGGGVGLAVARLA"
     misc_feature    complement(10480..10492)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
ORIGIN
        1 tcctgcaccg cgtcgtctcc tctcgccccg tggaggtccc cgtgttcccg gccgcgggga
       61 cctgtggtgt gtcccggggc cgggccgggt cgtgccgctt tttcggccgc ccccggcggg
      121 cgggggtggt cgggcgtcag acccgtacga gccgttcgcc gccgggctcc gcgtgggcct
      181 cggccgggct ctcgggtccg gccatcagga tcgagcgctg caggcggcgc agggcgatgg
      241 agggttccag tcccagttcg cgcacgaggg tggcgcgcag tcgctggtag acgtccaggg
      301 cctcgccgcg gcggccggag cggtgcaggg cgagcatgaa ctgtccgtgg aggttctcgt
      361 gggtgcggta gcgggtgacc aggacggtca gttcggccag gagttcgcgg tggcgtccca
      421 gccgcaggtc ggcctcgatc cgctggtcca gggcgcacag ccgggtctct tccaggcgtc
      481 tggtctccat gtcgagctgg attccgccct gtacgtcggc gaaggcgggt ccggaccaca
      541 gggccagggc ctcgcgcagc aggcgggcgg cgccggggaa gtcgccggcg tccatggcgc
      601 ggtagccggt gccggcgagg cggtcgaact cgcggacgtc gagggttccg ccgccgctgc
      661 tgaggaggta tccgccgggc agggtgacca ggatgtcctt ggcggtccgc accacccgcc
      721 ccgcccccgt gcccgcggcc gtaccggtgg gggtgtcgtc gtccccggca ccggggccgg
      781 ggccggtggc ggtgccgcgt tcgagggcgc cggcaatgag ggcgcgtaac tgcaggacgt
      841 aggtctgcag tgtggtgcgg gcactgcgcg gcggttcgcc cgcccacagc tcctctatca
      901 gtgccgatac gggtaccacc tgatcggcat ggagggccag gagggccagg acctgccggg
      961 gcttgggggc ggtcggcgtg atggagatcc cgttctcccg caccgccaac gcgcccagta
     1021 catcgatgtc cacgccgtac tcccctgtcc ttggatgagt cgcacagcca gtagaaaaca
     1081 ggaccggcgg tttgtcaata tcaaaccggt cgtgctgttt tgatggtcgg tcatgtccga
     1141 cggaacgtca gatacctttg ctggcctgcg atttcgggtg ggtccgggtg cggtggggtg
     1201 gcttcaggac gcctcgatcg cgcctcgacg agggccacag gagggtggtg gaccccacct
     1261 gaaagagacc gctcagtctg attcggtgtc cgggtcgggg ggtggggcgg cgctcccggg
     1321 cgatccctgc ggcagcaggg cgtccagggc cgggggcgag gcgagccgcg gcagcatcag
     1381 cgtccagaac cgggtcaggg tgtgcggtgc gagccactgc cggtcgtagg cgcccaggac
     1441 ctccagtccc accgtcgcgg ccaccacccc gcgcgccgcg tcctcgggca ggagcccctc
     1501 ggcgagcagg ccctcccggg ccgcggccgc cacggccgcc tcgacccaca gccgccactg
     1561 ccggcgcagg tccgccgtcc cctcgaagga ggggtccgcg cacagcgcga acccggcccg
     1621 caccaccgcg tcgtgcgcaa ggagggccat cagccggtgc gtggagtcca ccagcacctg
     1681 caacgaaccc ccgcccgaac cggcacccgc acccgcgccc ccgcccccgc ctgggtctgc
     1741 gccggtgccc gtgccggtgc cggtgcccgc gctcccgtcc gggcccggga cggaggcggg
     1801 ggtggtcgct gcggaggcag ggaccggggc gggggtggtc ggtgcgggtg ccggacccgg
     1861 cgcggaggcc ggacccggtt cgggggtggt cggggtcgag gccggacccg ggtccggggc
     1921 tggggcgggg gcgggggtgg tcggtgcggg gatggtggtg gtcggggtgg tggcggttgg
     1981 cggcggtacg gccgggccgg ccgtgatgcg cgacagggcc tgcgcggccc ggtcctggac
     2041 ggcgcgggcc aggacggtct tgccggcgaa gtggaagtgc agtcccccgg cgctgacccg
     2101 ggcccggcgg ctgatcaccg cgatcgacgc ggcggtgaac ccctcctcgg cgaacacctc
     2161 cgcggccgca cgcaccagcg cctcccgcgt acgggccgca cgttcctgct tgaccatgga
     2221 gtctccacaa accgccaacg cggtttgccc aagggggtgt aaagatcgtt ccggtcacgg
     2281 tccgcgccgc caccggagcc ccgctcgcgt cccgctggag ccggccggcc ccaccggccc
     2341 cacccgcccc accgggggcc gccgtcgggc gggcagaggg gcgggcgggc ctcaggacag
     2401 ggccggttcg ctctcctgcg ccgccccccg gccgggcccg cgccgcatct ccgcgctcac
     2461 ccgctccccc cggtcgccgg cgatgtcgag cctggcgagc atcgcgggcg tcgcgatgct
     2521 cgggcagatg tgctggtaga cggcggccac ccgctgctcg atgtcgctgc ggtcgctcag
     2581 ggtctgcgag tagatctgcg cgcccgtcca gcaggcggac aggatccagg ccgtctcggc
     2641 gggatccacg tgcggcagca gctcaccctg ctccttcgcc cgcaccagca gggtccgcgt
     2701 gaactcgatc cagcccggca ccgaaccccc gccgaagatg tcgcgcatgc ccggccccag
     2761 agagagccgg gcacccgcgc tgagcatggg ctcgcgcttc atccggtacg ccagcaccat
     2821 cgcgacgtcc accagttcct gcagcttgct ctcccggtgg ggcaccccgc cctcggcgaa
     2881 ctgctcgtcc aggacgccct gcgccagctc ctgcttggag gagaaatgga aatacagggc
     2941 gcccttggtg acgcccgccc gcgcgaggat gtccgcgatg gtggccgcgt cgtaaccgcg
     3001 ctcgtcgaag accgccgccg ccgcttccaa gatcgcgcga cgcgtacgta ccgcacgctc
     3061 ctgccgtgcc accggtgcct ccgttgtcca tggtccaata cggccggatc caccgcgcgg
     3121 atccaccgcc ccattaaaac caaccagaag gtccgattct tttcaagcag gccaggcgtc
     3181 gccccgacgc ggtcaatcgg ccacgggcgc cctgggaccc agcaccacgg gcaactcccg
     3241 cagcccgttg gagatgaacg actccacccg ccccaactcc cccacccccg ccagagacgc
     3301 cgccgggaac gtctcgaaga accgccgcac cgccaccgcg cactccagcc gcgccagcgt
     3361 cgcacccagg cagtgatgcg tgccgaaccc gaacgacagg tgctgtcccc gcgtcgcccg
     3421 cagcacgtcg aagcggtcgg cgtccgcacc gtgcacctcg ggatcacgcc ccgccgaacc
     3481 gaaggagatc aggatcggat caccctgccc gatcaccacc ccctccccca tgtcgatgtc
     3541 ctccaccgcg aaacgcagca gcccgtagaa ggccggcggc tcgatccgca gcgcctcctc
     3601 caccacgtcc tcccacgaag ccaaccccgc ccgcaccagc gccaactgcg ccggattgcg
     3661 cagcagttcg tgcagggccg tgcagatcag gttgacggag gtctcgtaac cggccgtgaa
     3721 aagcaggatc aggctgtcga cgagctcctt ctcgctcaac cccgccccct ccacaccccc
     3781 acccgcaccc acatcggcgg cggcggtagc ggtacgggcg tcggtggcgg tgcgggggtc
     3841 ggtggcggtg tcgcgggcgg cgatgagtgc gctggtcatg tcgtcgccgg ggtggcggcg
     3901 gcggtgggcg accaggtccg ccatcaccac gtccagatcg gccgcgttgc gctgcgcctc
     3961 ctcggcggtg ataccggtgt cgaagaacgc gttgatgatt ttgtgcagcg ggccccagga
     4021 gtgctccggt atcccgaaca gctcgaagac gatccgggtc ggcagctcgt gggcgaaccg
     4081 cgtgcgcagg tccaccgcgc caccgggcgc ggaggcggcc aggtcctcca ggagttcacc
     4141 gaccacctcc tcgatccggg ggcgcaggcc ggccgcccgg cgggcggtga acgcggccgc
     4201 cgccggtctg cgcagccggg cgtgctcact gccgtaggcg gtgatcatgt tctgcaccga
     4261 gacccagatc gccagcggcc actgctcccc cacctcgccg ctgatccagg ccggccagtg
     4321 gcggtaggcg tccttggaga cccgcgggtc caccaggagc cggcgcatgt cggccgagcg
     4381 gcccacggcc caggcactga ccccgcccgg cagcaggacc cgtgtcgcgg gcccctgttc
     4441 acgcacgcgc gcggcctcgg cgtgcacgtc cccgcccagg gcgtcgatcg caaaagggca
     4501 ctgacggttc atctcacaac tctccaggat ggacggcggc agatcggcga cagggtgcag
     4561 cggcgtgtcc acaccccgta ttccaaaacc gcccatccgg tttctgtttc accggcggcg
     4621 gccccgagtc tgacacgacc cgacccgcat gccaatggtt ttcggggccc ggcccgccgc
     4681 atccggtccc ccgaagtcgc cctggcgcgg gcctggttga acggacggcg cccgcaccct
     4741 gtccgaccgg cgcatatgac cggaaccacg gttcccactt ggggcggtgg tgtgcgcgtc
     4801 ccctgcggcc gcaccctgtc ggatatccgc tggtccaggt cctgacagcc ctcccggagg
     4861 gcgcccggcg ggccgccggg ccgacgcgcg ggccgcatcc tgcccggctg agctccttgc
     4921 aaacaaaacc ggagagtacg tatcttgcgt ctcgcgagcg caacccgtcc catccgtcga
     4981 acggctctgc gggagaagtc atgaccatgc tgacgatcgg aaagcacgcg cccgtcctgc
     5041 ccctgcccgg gacggagcag ccatggatgc gctgcaacga cgcccgccag ctgaccacca
     5101 ccgtgccccg ggaatacgtc caccgcagct cactggccga ggtcttcctg accggctgcg
     5161 aacgcaccgg ccaggacacc ttcaccctga ccgggcagtg gccccgcgcc cacaccttct
     5221 tcaccagcgc cgacggcacc ggccacgacc ccgtccaggc cggcgagacc gtccgccagg
     5281 ttggcctcta cctctgccac gccgtctacg gagtccccct cggccacaac gtcctgctgt
     5341 ggagcctgga cttcaccacc cgccccgaac acctgcgcat cggccgcggc cccagcgacc
     5401 tcaccatgaa cgccctgtgc accgacttcg gctggaaggg caaccggttc tccggctacc
     5461 tcagcgtcga catccaccgc aacggcgaac tcgccgcccg cggcaccgcc cgcttctcct
     5521 gcgtcgcccc cgccacctac cgccgcctgc gcggcgaccg cggccgcctg gccttgaccc
     5581 gccccctgcc ccgccgcacc cccgtccccg cccacaccac cggccggctc ctgcccttcg
     5641 acgtcgtcct cgcccccacc gaccaccccg gccgctggca actcaacccc gacctcgacc
     5701 accccatcct cttcgaacac gccaacgacc accaccccgg catggtcctc gtcgaagccg
     5761 cccgccaggc cgccagcgcc ctccacaccc ccaccacctt cacccccgcc gccatcgcca
     5821 ccgaattcca ccactacgcc gaactcgacg ccccctgctg gatcgacgcc accctcacca
     5881 cccccggcca cgtcaccatc accggccacc aggaaaaccg caccatcttc cactccaccg
     5941 tcaccgcaac caccacctga cccaccccgc ccgggggaca gcgggcgcag cggggcatcg
     6001 ggagccgggg gcgggggcgg gccggcaggc acaccccgcc cgcacaggag cgggaacacc
     6061 tccctgggca acggcttcac caccacgacc acgaccgggc aacaccccac cccgcaacta
     6121 cgttcacacc gggggcgggg cgggggcggg caggcgcaca cccgcccgca agggggggga
     6181 acacctcccc ccggcaacgg ctcagccacc gcgaccgcat ccgcgaccgg gcaacacccc
     6241 gaccacgcga caacgttcac atcggggggc cgcccctgcc cgcacaccac cagcccagcc
     6301 ggcgccgcag gaacacccgg ccggaacacc ggccgggacc cccctctgcg gccccgaacg
     6361 cccccaccac gaggcgcagg cacacccaac aaaggggaag ccgacccggg caaccgaccg
     6421 gatacctccc ctcgcagcag cccgcgccca tgccaggcct ccgcccctga accagggggc
     6481 gcggacaccc gggggcacag gggatggact cgcatcggct gcccggggac aacacccttt
     6541 accgaaaacg acgttcacgc ccgccgcagc catgacggcg ccacgagcga gcgcgcccac
     6601 cgaagccggg cccgaccccg tccccgggca ccgcgccggc cccctcgctt cagcctctgt
     6661 ccacgccggg cccccgcagc agcccgtggt tcacgccagg accattgccc cgcaccacgg
     6721 cgcaggcagg cacgccgcac cagagtgtcc ggcccggacc ccggaccggc tccgcacccc
     6781 agccgtcccc gggaaaccgg gcacgcagcg gccgaacaca caatgcggcc gcccggacgg
     6841 ggccggtcgg accccctgca gcaaccgcag cccacaccaa cgccccgccc cgccccggca
     6901 cggcccggcc cggcacgcgg gggcgcagtc cccgaagcgg aggaggctca tccggggacg
     6961 gccgacccga cagcctcccc caccacagcc gatgttcacg ccggagccgc cgcccggacc
     7021 caggggcttt catgcaccga aacagggggc atccacaccc gttcgagcgg ccggacagcc
     7081 ccgcacggca gcccccgttc acgccagggc ccgtaacggg acccccgaag gggcgcaggc
     7141 aacggaacgc aacggcacgc aacggcacgt aggggcggcc cggcccggac accggaccgc
     7201 acaacacccg gcgcagtaca cgatgcgcac ccgcaggccg taccggggca ggggagcgcg
     7261 ggcaccggac ccggatcccg ccccgaacac cgggacagac acgcccctgc ggcagccgat
     7321 gttcacgccg ggggctgcca cccggcacga aagttcaggc acgaaaccag gggcccgccc
     7381 gacccgggag accgacccga cagcacccgc gcgcgcatcc ggcgtcccca cacggccacg
     7441 ccccgggaac ccggcccccg gacagcaggt cacccggccc aggacctccg accggccacc
     7501 ccctcacggc aacccgcgcc cacaccaggc ccccaccccg gaaccagggg acgcagaggc
     7561 accgggaaaa cgatgacccg acccggctac cccggcccgg caacacccct ggcagcagca
     7621 gaagttcacg cccaccgagt ggggtgtggc ggcatcacgg gccgcgggca ccgaagccgg
     7681 acgccccccc gcccccgggc accgcaccgg agccccctcg cggcagcccg tacccgcata
     7741 cggcgacaag ggctcagacg ggtacgaagg cacgtgctca gccgtggggc ggcggggacg
     7801 gcacccctgc ggcgacaggg gggtgtccgc aggcgcccgt gacgcaccgg cgctccgcac
     7861 ccgcaggccg cgggcgtagg ctctgcggga tgcggatagg cgagcttgcc cggcgcacgg
     7921 gcgtgagccc gcgctcactg cggtactacg aggaccaggg gctgctgacc agctcacgct
     7981 ccgacgcggg gcagcgtcac tatgccgatg ctgcggtcca acgcgtgacg ctcatccgac
     8041 agttgttcga cgcggggctg tccagccggg tgatcgcgac cgtgctgccg tgtgtggaca
     8101 tccccggtga cccgggcgtc gccgaggaga cgttcacggc aatgatgcgc gagcgcgacc
     8161 ggatcgacgc cgacatcgcg cacctgatcg agacccggac tgcactcgac gcactgatca
     8221 gggccaacag ccggcaccgg gcgcagctgt ccacggcccc cagaaccggc gacgcagccg
     8281 acctggtgct gtgacctgca tctcatcgcg ttgcccctga catcaatggc aggggtgagg
     8341 atggcgacag cgcccgcaat cagcggggtc ggtcgtacga gaggcggcgc aagatgggac
     8401 aggcatgggg tttcgacagg cacggcgggc ccgaggtgca ggcgttcttc gagcgccccg
     8461 atcccgtacc cggtcgcggt gaggtactga tccgggtcga cgtcgccggc gtcaatcccc
     8521 tcgaccacct gctgcgctcc ggcccggtcg acgggctcga ggacgggcgt ccctttcccc
     8581 gcgtgctggg catggaggca gccggaaccg tactcgcccg gggccaggac gtcaccgggc
     8641 tcgaggtggg cgacgcagtc ttcggcttcg cactgaccgg tggcggcacc tacgcccgga
     8701 ccacggtgct gtccgcggcg aacaccgcgc gcatcccggc gggcctgtcc ccaaccgtgg
     8761 cggcaacgct gccggtggcc gggacgaccg cggtcgacgt cctcgaccaa ctcggactcc
     8821 cggccggcgc cacggtcctg gtcaacggag tcggaggcgg agtcggcctc gccgtcgccc
     8881 ggctggccat cggacgccgg ctgcacgtga tcggcaccgg gagcaccgcc aaacgcgaac
     8941 acgccgagag cctcggggcg ctcttcatcg actacaccac ccaggacgtc gccgccgcgg
     9001 cacgccagct cgttccggac ggcttcgacg cgatcgtcga cctggtcgca ggcacctcac
     9061 tgcaggcggt cgctcccctg gcccgcgacc cccgcaacat cattgccgtc agtgatactt
     9121 ccgtggccgg tctcggcggg cgcttcgtcg agcgccgcct cgaccgcgag aacctcgagc
     9181 gatcagcccg actcgccctc gacggactcc tcgcacccgc aatcaccgca gtccatccac
     9241 tctccgaagc cccggccgcc ctcgccgccg tcgagaacgg ccacaccacc ggcaagaccg
     9301 tcatcaaggt gacatgagaa acacccggcc acccgacaac atcaaccgtc caacgcggac
     9361 cggcaaacac gacaaccatc ggcaacggag ccgggcggac gcgacccagg gtcggcgcat
     9421 caccgacacc caccacgctc acccgtaccg aatcgaacac cttcacccat acgccaccag
     9481 cccgtgccca caccaggcca ccccgcaacc acgagccgaa gacaccgaac accaggaaga
     9541 cccagcccgg ctatccggcg gacaacacac ctcgtgacac acaacgttca caccctgcca
     9601 aacccgtgac ggcgcccagg gcacacaggc caccgcaccg aaaccacggg ccgcggcgac
     9661 aacaccccgt gcatcagcag acgttcacac cccgccaagg ccctgcccgc ggcactgaac
     9721 gtctcccgcc gtgacaggca tccgccgggc agccggcgtg accgcgccag gacacaccgg
     9781 ctgccgcgcc gaaaccatgg ggccaccgcc ccacggcacg acccgccccg acggccgggc
     9841 atacaacacg cctcgcgaca ctccatgctc acgcccgcca aagccgtgcc agcgccggcg
     9901 gacgcacgga cgccgaagcc ggaagccacc cggtgcgagc gccgcaccgg ccccacacga
     9961 cagcccctgc ccacaccaga ccaccacccc acaacccagg gtccgaagac accccacaca
    10021 gcaaagaccc gaccccggct gccccacgga caacaccccg tgcatcaaca gacgttcaca
    10081 ccccgccaag gccgtgccag cagcgctgaa cgtctcccgt cgtgacaggc atccgccggg
    10141 cagccgacct gaccgcgcca ggacacacgg gcgccgcacc ggaaccacgg gccaccgcct
    10201 cccggcacga cccgccccga cggccgggca tacaacacgc ctcgcgacac tccatgctca
    10261 cgcccgccaa tgccgtgcca gcgccggcgg acgcacggac gccgaagccg gaagccaccc
    10321 ggtgcgagcg ccgcaccggc cccacacggc agcccctgcc cacaccagac caccacccca
    10381 caacccaggg tccgaagaca cccgacacag caatgacccg ccccgctgcc ccacggacaa
    10441 caccccgtgc acagcggacg ttcacacccc gccaaggccg tgccagcggc actgaacgtc
    10501 tcccgccgtg acaggcgtcc gccgggcagc cggcgtgact gcgccaggac acacgggctg
    10561 ccgcgccgga accacgggcc accgcctcac gaacgacccg ccccggcggc cgggcacaac
    10621 acgcctcgcg acacgccctg ttcatgccca ccagggctgt gacggcgcca gcggcgcaca
    10681 gacaccgaag cccgggcccc gcaccgaagc cccacccgac agcccgcccc cacgccgggc
    10741 cacccggccc gcaaccacca gccgcagaca ccccacacca caacgacccg acccccagcc
    10801 ccagccaggg ccccggcccc cgagccccag ccccagcccc agccccggcc ccggcgcccg
    10861 ggccgggccc gagaaggggg agggcatcgg ggccgtcagg cagggttccc gcccgacagg
    10921 accgaccggt accacggagc acacgccgcg aacgcctcca
//
