LOCUS       NZ_KV917378            20822 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Salipaludibacillus agaradhaerens strain DSM 8721 Scaffold1, whole
            genome shotgun sequence.
ACCESSION   NZ_KV917378
VERSION     NZ_KV917378.1
KEYWORDS    WGS; RefSeq.
SOURCE      Salipaludibacillus agaradhaerens
  ORGANISM  Salipaludibacillus agaradhaerens
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae.
REFERENCE   1  (bases 1 to 4319439)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Genome sequencing project for genomic taxonomy and phylogenomics of
            Bacillus-like bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319439)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2017) Agricultural Biological Resources Research
            Institute, Fujian Academy of Agricultural Sciences, No. 247, Wusi
            Road, Fuzhou, Fujian 350003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 227.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002019735.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 15:23:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,010
            CDSs (total)                      :: 3,909
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 101
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 17 of 38
            Pseudo Genes (incomplete)         :: 21 of 38
            Pseudo Genes (internal stop)      :: 7 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:59:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3833434
            Orig. end                         :: 3854256
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV917378.1.
            Bacteria and source DNA available from DNA is available from: DSM
            or Fujian Academy of Agricultural Sciences.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20822
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="7"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20822
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20822
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10822](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10822)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(685..696)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.54"
                     /tool="antismash"
     gene            751..2490
                     /locus_tag="BK581_RS17630"
     CDS             751..2490
                     /EC_number="2.7.13.3"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018305.5"
                     /locus_tag="BK581_RS17630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_078579394.1"
                     /transl_table=11
                     /translation="MLMFRKYRIHSIFFASFFIFTVSILIVIIFFSYHYSSTQIIDTTT
                     EYQKNNLYRLSEELGDNLKSFQDYSVILSRQHNFRQVISGRQSVREQASGRTSLTNDFS
                     NVIYSVQALHTVEIFMSSPPVDNIQFPVRYSELASIFEKEWFQDIEDSAYSWLGHREVD
                     TTAGPTEVISLGRKINTSNGDLSAVMIINLDPLIVENWLRKDYEDSHIVLLDHMGTIVA
                     STNQEEIGGNEYGGLINGSQESFFDSPSQSQRQLDNNLVVTSSIPSVNWTVIQITPYEE
                     LTEGSVNMAKKLGLIGVVGVLIVLIVTFFLTKKFTYPILQLANVMKSYRLTQTKVSLPQ
                     GYRNEFGELFEGFEELTNNMEELYKSLDDQHHRQRKAEIKALQANINPHFLYNTLDQLN
                     WMAIEKGNIDISRMLELLGKMLRIGLSKGESIITIRDELKYLEYYMNLQKIQLEDSLTY
                     DMRVSDNVLNYYIPKLTLQPFVENAIIHGFRDRRHGVIMIDMKENAQDLILYVIDNGIG
                     FSKPPLLTNSQQHLGGYGIKNVIERLNVYFGDKADVQIDSEEGRTTVSITIPKVSNKSH
                     LSA"
     gene            2664..4322
                     /locus_tag="BK581_RS17635"
     CDS             2664..4322
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012301.5"
                     /locus_tag="BK581_RS17635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_078579395.1"
                     /transl_table=11
                     /translation="MWKVVIVDDDDKVLRGMRNIIPWEELSCEWVGEARNGEEGHHVIM
                     DLEPDLVITDIYMPVMNGLEMIEQVRASGLNTRVIILSGYNEFEYARQAMRLSIDDYLS
                     KPASPDTIKDVLISCVNSLEKEAIKQMEVSELRERVALYEPLVEKEWVKAVITGTTTHL
                     TNLPRTLDQLTRRWSQQQHVIMTISYDQSLEASSLYKSGWYLFRFATENVINDAVNKYI
                     DDSHYIELHSHQTALCLHINQTKGKVNDELLNHIKHDIVTKLDTYFKLKVRVGFGCFKN
                     EWADMAISLKEALMDIDQEVQEVTGLYNEFMFNEETKLNSKSLWLDSIENIHRLSEAIR
                     YADKASAQHVIQAAYFYYKDHPFNMSEVMRAGIEMWTIMTYSLYDIGIKIDEMFSDTFD
                     FHSGLTKLSHWKEFKNYMNDLISHICDHQQWDENLKHRQLIEQMLDYIHKNISKNITLQ
                     DIANELYISRNYLGQIFKKVVGESFKNYLTRVRMEKAKKMIQEGDHLIYEISEKVGFIN
                     PAYFTTTFKKYTGYTPSELINRKLMAAHEQTVQDA"
     gene            4674..5429
                     /gene="kduD"
                     /locus_tag="BK581_RS17640"
     CDS             4674..5429
                     /EC_number="1.1.1.127"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (10-126). E-value: 4.9e-09.
                     Score: 28.3. Matches aSDomain:
                     nrpspksdomains_BK581_RS17640_PKS_KR.1"
                     /codon_start=1
                     /gene="kduD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013171426.1"
                     /locus_tag="BK581_RS17640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="WP_078579396.1"
                     /sec_met_domain="adh_short (E-value: 3.5e-32, bitscore:
                     109.7, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEMFSLEGKTAVVTGATRGLGQGMALGLAEAGADIIGISYLPQK
                     ETQKEVEALGRQFFDINADLSIQDELASVVKDAISKTGKVDILVNNAGIIRREQAENFS
                     DRDWDDVVNVNLNAVFRLSRDFGKHMIENGFGRIVNVASMLSFQGGLKVPSYSATKHGV
                     AGLTKSFANEWGSKGINVNAIAPGYMVTDNTAPLRADKNRSDYITSRIPKGEWGTPEDL
                     KGPVVFLASEAGRYVNGHILCVDGGWMSS"
     aSDomain        4704..5051
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK581_RS17640_PKS_KR.1"
                     /evalue="4.90E-09"
                     /label="BK581_RS17640_PKS_KR.1"
                     /locus_tag="BK581_RS17640"
                     /protein_end="126"
                     /protein_start="10"
                     /score="28.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TAVVTGATRGLGQGMALGLAEAGADIIGISYLPQKETQKEVEALG
                     RQFFDINADLSIQDELASVVKDAISKTGKVDILVNNAGIIRREQAENFSDRDWDDVVNV
                     NLNAVFRLSRDF"
     CDS_motif       4710..4775
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK581_RS17640_0001"
                     /evalue="1.10E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="BK581_RS17640"
                     /protein_end="34"
                     /protein_start="12"
                     /score="11.7"
                     /tool="antismash"
                     /translation="VVTGATRGLGQGMALGLAEAGA"
     gene            5875..7026
                     /locus_tag="BK581_RS17645"
     CDS             5875..7026
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429953.1"
                     /locus_tag="BK581_RS17645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="WP_211273985.1"
                     /sec_met_domain="Fe-ADH (E-value: 2.3e-123, bitscore:
                     409.4, seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMGKVFHVPETNLMGCGCLAEIGVYISELNVKKALLVTDSMLSKN
                     GVVDKVIDVLKKANIAYTTFDHVKPNPTVQNVTDGVRHYKDANCDFIISVGGGSPQDAA
                     KGIGLMITNGGDLRDYEGVGKTTYKSVPIVAINTTAGTSAEYTINYVITDEERHVKMVM
                     VDKNSLVKISVNDPELMVSKPSNLTAATGMDALTHAIEAIVTPGAYTVTDATALSAVEI
                     IFYYLPRAVADGKDMEAREQMVYAMFLTGMAFNNAGLGYVHAMSHQLGGVYDLPHGVCN
                     AMLLPVVEKENAKRHPDKFPLIAKAAGINIEGKTPDQCADEVIKKIKALSKVVGIPTRL
                     SELGIDSVDINLLAENAMKDACASGNPFIPTRQEVIDMFKEIV"
     gene            7047..7877
                     /gene="rhaD"
                     /locus_tag="BK581_RS17650"
     CDS             7047..7877
                     /EC_number="4.1.2.19"
                     /GO_function="GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rhaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744740.1"
                     /locus_tag="BK581_RS17650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="WP_078579398.1"
                     /transl_table=11
                     /translation="MTETVKKTILSADFMQDMIKTTTNLYRLGWDERNGGNISYLLKEE
                     EVAAFTDVNQTIKTIPLNFHIPELSYHYFLVTGSGKYFKNIVDAPEENLGIIRINEGGE
                     TAAIIWGFSHGTLPTSELPSHLMAHKERLARDASHRVVMHTHTTHLIGMTFTHTLIEKE
                     FTRTLWKMCTECIVVFPEGVGIIPWMIPGTDEIGKATADKMSDVRLVVWPHHGVFGTGN
                     TMDEAFGLIETAEKAAEIYSLVEGKIKQTITDLQLYDLAQAFNVKPREGYLNET"
     gene            8189..9874
                     /locus_tag="BK581_RS17655"
     CDS             8189..9874
                     /GO_component="GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1061: methyl-accepting
                     chemotaxis protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095591.1"
                     /locus_tag="BK581_RS17655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_078579399.1"
                     /transl_table=11
                     /translation="MKSSLAIKLNVLIFSILILFSIVLGSVLYKQITDGIKEAAVEKAA
                     SDLELGFRYIDKAIPGEWYVEEGVLYKGDTPMNENYDLVDEIGDMTGGTVTLFLHDTRI
                     ATNVILDGERAVGTQVSEEVAEAVLSREEIYLGEADVAGYSYQTAYMPITSANGEVVGI
                     WYVGASQEFIDSTVKSAMQGFLIVLTAVMVVAVGAVFVFTKRLTRRLSKLTDGVKAAGE
                     GDFSIQFSDTSKDEFGQLAGSFNTMVKKIHRLLSNVSHLSQQVAASSEQLTASAEETTK
                     ATDQISESVQNVAAGTEKQLEKAVVSNEVAETISFEMENISHHIEQANEKANRTSKTAE
                     EGGKVVDQTLMKMKDIQDKSESGVQVIRSLSNKSVKIGDILSVISGISEQTNLLALNAA
                     IEAARAGEQGKGFAVVAEEVRKLAEESGDSAKQISTMIGEIQEGISESVTAMESSRTSV
                     ASGIENADKAKETFKDISEAIKDVSDQVMNVTEATQHVTTNIGKMVTSINESKLITENA
                     AGYSQNVAASTEEQTATMEEIASSAFTLAKMAEDLQKAVSAFKL"
     gene            complement(10001..10822)
                     /locus_tag="BK581_RS17660"
     CDS             complement(10001..10822)
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415444.1"
                     /locus_tag="BK581_RS17660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="WP_078579400.1"
                     /sec_met_domain="PT_phytoene_like (E-value: 3.6e-30,
                     bitscore: 103.0, seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="phytoene_synt (E-value: 1.4e-14, bitscore:
                     51.8, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNEKQLHKEAMGQLKKTSRTFYIPITLLEPTLKKTVASAYLCMR
                     AIDEIEDHENLEPATKSHLLSKTSVLLSDTFNNDAYLELIEPYKQLLPEVTIRLGDWLS
                     VCPADIIEKVQESTSIMAQGMAKWAEKNWDIQTKEDLDDYTYYVAGLVGVMLSDIWEWR
                     DGTKTDRDYAIAYGRGLQTVNILRNQEEDKDRGISFIPKNWNIADTFQYAEANLALGDE
                     YMKDITSKNVSLFCKIPLELAKRTLHVLKSGREKMSRDEVNQTVEDIKNYH"
     misc_feature    11034..11045
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 21.4"
                     /tool="antismash"
     gene            11320..11676
                     /locus_tag="BK581_RS17665"
     CDS             11320..11676
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK581_RS17665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_078579401.1"
                     /transl_table=11
                     /translation="MSQKIQAYFKTENDAEKAVAHLQKLNTSNVIVDELPEGGRSLILF
                     PVANLGTTGSGMGNFGAPGFFGSKEEIKSMGSNKSETFTHIVEFNISEDEAKEAFATLK
                     EADAYIDSELNSSP"
     gene            11903..13408
                     /locus_tag="BK581_RS17670"
     CDS             11903..13408
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S41"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015531.5"
                     /locus_tag="BK581_RS17670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S41 family peptidase"
                     /protein_id="WP_078579402.1"
                     /sec_met_domain="Peptidase_S41 (E-value: 4.8e-11, bitscore:
                     40.4, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMTWIISLLILCSLIAGCSEVVEPISDIEKLRDITIEERFSESVR
                     RDEIEKYLHKENGDNEIESDDLNMLKVSNGRAETVTKEEMLLDSHLLNQIWSHSYALYE
                     VYGGQDSFSQAHELLTATIEDEYSEGEEANYEEFIALISDHYAFITDQQLLIDGSPFFD
                     REHQFYITEELTFVKDDNNNYWLYKDNHKKDKVVSINGDDPDVYLLPSLNFEGELVYFP
                     GMYTDYLPQKLWDIDLENQETIEVTVRQSGLDRLRDTSFDVYEDEEVPIIQLRNMLVYK
                     EDSYNYEDMLGSVDQVEDESTFILDLRNNNQGHIKFAQEWYKRLTSHEPSIKSYSAQLM
                     TPTTITFLEDKVERNREAGVEWGGLLDTEDPFNINEYHDVTRLQWSIEEMETDGITELD
                     STIYILIDEATSASAEILVAYLRQHPNVYIVGAPSEGAMSSGNSQLIELPHSGAKVSIP
                     SMFRYHPQFLEKEAIGIEPDLWVYPRFARERVVKWIDNNNDDL"
     gene            complement(13678..14991)
                     /locus_tag="BK581_RS17675"
     CDS             complement(13678..14991)
                     /EC_number="3.2.1.86"
                     /GO_function="GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009429.1"
                     /locus_tag="BK581_RS17675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="WP_078580011.1"
                     /transl_table=11
                     /translation="MKKGLKIATIGGGSSYTPELVEGFIKRYDSLPISELWLLDIPEGE
                     EKLTIVGNLAKRMVEEAGVPMTIHLTLDREKALKDADFVTTQFRVGLLDARAKDERIPL
                     KYDVIGQETNGPGGLFKGLRTIPVILDIIQDMERLCPHAWLINFTNPAGMVTEAIYRHS
                     NWRKVVGLCNVPVGIKMGVAKALDVEANRIHVDFAGLNHMVYGMDVYLDGKCVTEDVIN
                     KITSGDSSMTMKNIVDIGWEPGFLKALNMLPCPYHRYYYKTSDMLAEEKKNAAEEGTRA
                     EVVQQLEKELFELYKDETLRTKPEQLEKRGGAYYSDAACNLIDSIYNDRGDIQPVNTLN
                     KGAIGSIPDDSAVEVNCIITKDGPKPLAVGDLPVSVRGLVQQIKSFERIAAEAAVSGDY
                     DQALLAMTINPLVPSDTTAKLILDEMLEAHKEYLPQFQ"
     gene            15155..15877
                     /locus_tag="BK581_RS17680"
     CDS             15155..15877
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524727.1"
                     /locus_tag="BK581_RS17680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="WP_078579403.1"
                     /transl_table=11
                     /translation="MFTPKQLARFSDLDTLLYHYILAHGEKVAYMRIRELAEVTHVSPT
                     TILRFCKKLDCDGFSEFKTKLKLYLEKAETKWLNHSYESMNEFLERTLKSDYQAQLSAI
                     ADEIVSSSQVVFIGSGSSGILAEYGARYFSSLNTFSSAISDPFYPINGKQIRHSLAIIL
                     SVSGETQHIVSKLHRFREEGATIVSITNTKSNTVAQLADYPLAYYINTEFNDISNVTTQ
                     LPVLYLIETLGKIVFEKL"
     gene            complement(16172..17149)
                     /locus_tag="BK581_RS17685"
     CDS             complement(16172..17149)
                     /EC_number="1.13.12.-"
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330344.1"
                     /locus_tag="BK581_RS17685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nitronate monooxygenase family protein"
                     /protein_id="WP_078579404.1"
                     /transl_table=11
                     /translation="MELPQLKIAQMKPKVPIIQGGMGVGISLSNLAAAVANAGGMGIIS
                     GTGIPIEEMKGHIRRARKLTKGIGYIGVNVLYAMTDFADTMKAALEEKVDFIISGAGIS
                     RDMYAWGKEYNTPVISIVSSGKLAKMSQRLGASAVVVEGFEAGGHLGTDRPLFDILPEV
                     VESVDIPVIAAGGIINGNDVKKALSLGASGVQMGSRFVASTECDAPQSFKEKYLSSKKG
                     DTTLITSVVGLEGRAIKNTFTNKISDGKKVKIEKCMSCLKKCSHRFCILDSLLKSRAGD
                     AEEGLVFSGSRVHEINDILPVKNIIDNIIKEYRDLTSTLSNQPT"
     misc_feature    17293..17307
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.3"
                     /tool="antismash"
     misc_feature    complement(17467..17482)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     gene            17547..18527
                     /locus_tag="BK581_RS17690"
     CDS             17547..18527
                     /EC_number="1.14.19.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) delta12_cd03507"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019420921.1"
                     /locus_tag="BK581_RS17690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="fatty acid desaturase"
                     /protein_id="WP_407690354.1"
                     /sec_met_domain="delta12_cd03507 (E-value: 2.2e-95,
                     bitscore: 317.9, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPFEKTERKISIKQMINTLGPLVILWYLAYLSLEVSYWMTIPLL
                     ITAAGFLVRTFIIFHDCCHQSFFKSRKANDILGTITGILTLVPYQQWKNSHNIHHATSS
                     NLDKRGTGDMWLLTIEEYAAASIWRKIAYRLYRNPVVMLGLGPLAVFCIDYRFNTKGAK
                     RKERLNTYVTNVSIVALYGALIWAIGWQSFLLIQGPILLVSGMLGIWLFYVQHQFEDTY
                     FEHDDEWSYVQAAVEGSSYYKLPKVLQWITGNIGFHHVHHLSPRVPNYYLEKAHEATPP
                     LQKATTITIATSFKSLRYRLWDETTKSFVTFKEAKKLMMKKTHLA"
     gene            18631..19746
                     /locus_tag="BK581_RS17695"
     CDS             18631..19746
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758573.1"
                     /locus_tag="BK581_RS17695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_078579406.1"
                     /transl_table=11
                     /translation="MKNWTKKFRKNTGLSPYVWVVFYILPFYFILQDSSTLETMIGIVM
                     TIVFFLCYVLSFASHGWLVYLGASMQIAISTAMSLLFGYIYFFLFLAFFIGNLKNRVAF
                     FTLYIILLITTFGTSYYGFLMHSVFITQLPFVFLSIIAVILLPVNTYNKNKEEQLQGQL
                     NDANKRIAELVKLEERQRISRDLHDTLGQKLSLIGLKSDLAAKLVEKNPSKAQLEIKDV
                     QQTARIALKEVRELVMEMRGTKLNDEINRVEQILQAATIKFHLEKPDELPRIPLIIENV
                     VSMCLKEAVTNVVKHSQASKCAIIIKALKTELTITISDNGIGIGKSERNKNSGLQGMKE
                     RLEFVNGHLKIEDTNGTNVIINVPVISNRKR"
     gene            19752..20357
                     /locus_tag="BK581_RS17700"
     CDS             19752..20357
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007428925.1"
                     /locus_tag="BK581_RS17700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_078579407.1"
                     /transl_table=11
                     /translation="MIRIIIAEDQKMLLGALAALLDLEEDMTVVGSASNGKDAIELVYK
                     HQPDVCIMDIEMPIKTGLDAAEELKLKDIACKVIILTTFARTGYFQRAVKAGVDGYMLK
                     DSPSEELVNAIRSVMSGRRIYAPELVDEFYSTPNPLTERENDVLRLMADGKSTKEIADL
                     LHLTNGTIRNYISSILEKLEVSNRIEAITRFKEKGWFK"
ORIGIN
        1 ggtgggggag ccattttgtt cagctttaat acccttctct atatgggatt tggactgaga
       61 ttactatttg ggacgttatt agttatcctt tttatcatgt atttactcac gtcaatttat
      121 atatttccag tattagtgca ttataatacg tcccttatga agcatattcg ttttgcactt
      181 atgattggac tcgcttattt acctcattcg ttgttgattg gagttactat tgtgttgatc
      241 ggggttattt atcaggcttt tccaggtctc attatgttct atttaatgag ctttcctgta
      301 gcagtcattt tacctatcgt tttacatgtg tttaaatcaa tcgaagaaaa agaagtagta
      361 ccagaacgca tgtaacttgc ttcggttatt ggcacaccac ttcttaagtt aaaaaaatgg
      421 gttcaaatga actttcatca gtgggcgctt tcattctctc ccccctgatt ggtcgttgag
      481 tcaatcatga catgaccatc cgttatcttc cagcttaata gatttccttt gctctctatt
      541 ttgagatgcg tgctttaagg acggttatct gtgataaacc gcaaaattct acatctttat
      601 ttcgtgaatc atggtatttt agtgttagag ataatagacg atgtgcatgt gaacctaaca
      661 aatttattta caaaacctca ttatgataac gctttcttaa taaagccatc tcacagcttg
      721 tagattagtt ggagaggatg tgacagtcat atgcttatgt ttagaaaata tcgcattcat
      781 agtattttct ttgcaagttt ttttatattt acggtttcta ttttaatcgt tatcattttc
      841 ttcagctacc actattcatc aacgcaaatt attgatacaa cgactgagta tcaaaaaaat
      901 aatctctacc gcttaagtga agaattagga gacaatttga aatcttttca agactactct
      961 gttattttgt cacgacagca taattttagg caggttatta gcggacggca atcggtaagg
     1021 gaacaagcta gtggtcgaac ctcgttaacg aatgatttct ctaacgtgat ttatagtgtt
     1081 caagcgttgc atactgtgga aatttttatg tcttccccac cagtggataa cattcaattt
     1141 ccggttagat attcagaact agcgtcaatt tttgaaaagg aatggtttca agatatagag
     1201 gatagtgctt attcgtggtt aggacatcga gaagttgaca cgacagctgg acctaccgaa
     1261 gtcattagct taggacgaaa aatcaataca tcaaacggtg acttatctgc tgtcatgatt
     1321 atcaatcttg acccgctcat tgtagaaaat tggcttagaa aagattatga agattctcat
     1381 attgttctgc tagatcatat ggggacgatc gttgcttcca ctaaccaaga agaaattggg
     1441 gggaatgaat atggtggctt aataaatggt tctcaagaat catttttcga ttcgccatca
     1501 cagagccaac gtcagttaga caataacctt gttgtcacaa gttcgattcc ctctgttaat
     1561 tggacggtta ttcaaattac accttatgag gaattaaccg agggaagtgt gaatatggct
     1621 aaaaagctag gtctaatcgg cgtcgtaggt gtgctgatcg ttcttatcgt gacgttcttt
     1681 ttaacaaaga aatttacata tcctatctta caattagcca acgtaatgaa atcttatcga
     1741 ttaacacaaa caaaggtatc gctcccgcaa ggttatcgaa atgaattcgg tgagcttttt
     1801 gaggggttcg aagaattgac aaacaatatg gaggaacttt ataaatctct tgatgatcag
     1861 caccatcgac aaagaaaagc agaaattaaa gcgctgcaag cgaatattaa tccacacttt
     1921 ttatataata cgctggatca gttaaattgg atggcgatag aaaaagggaa tattgatatt
     1981 agtcgtatgc ttgaactact gggtaagatg cttagaattg ggttgtccaa aggtgaaagt
     2041 ataataacga taagagatga attgaagtac ttagaatatt atatgaacct tcaaaaaata
     2101 caattagaag atagcctaac ctatgatatg agagtaagtg acaatgtgtt aaattattat
     2161 attccaaaac tcacgttgca accctttgtt gagaatgcga ttattcacgg tttccgtgac
     2221 agaaggcatg gtgtcattat gattgatatg aaagaaaatg cgcaagactt aatcctttat
     2281 gtgatagata atggcattgg tttttcaaaa cccccacttt taacgaatag tcaacaacat
     2341 cttggcggat acgggataaa aaatgtaata gaacgtttaa atgtttattt tggagataaa
     2401 gcagatgttc agattgatag tgaggaaggg agaaccactg tctcaattac aatcccgaaa
     2461 gtcagtaata aatctcattt gtcagcttaa tttgattcat cacagagaac cgtccgaaaa
     2521 cgcccatctc aaaatacagc ggagataaat ttatttaggt gggatgtcct gattcacttt
     2581 actaccaatt ggtggcagaa gaacgaaaat gccactgatt gaagtttcgt tttataaact
     2641 ttgcttgtag ggaggccttc agcatgtgga aagtcgtgat tgttgacgat gatgataaag
     2701 tattacgagg aatgcgaaac attattccgt gggaagagtt gtcctgtgaa tgggttggag
     2761 aagcgaggaa tggtgaagaa gggcatcatg tgataatgga tttagaacca gatttagtca
     2821 ttacagatat ttatatgcct gttatgaatg gtcttgagat gattgagcag gtgcgagcct
     2881 caggattgaa cacacgggtg atcatattaa gcggctacaa tgaatttgag tatgctagac
     2941 aagcgatgcg attaagcatt gatgactatc tgtcgaaacc ggcttcacca gatacaatta
     3001 aagacgtact catctcatgt gttaatagtc tggaaaagga ggctattaag caaatggaag
     3061 tgagtgagct gagggaaaga gtagcgcttt atgaaccatt agtggaaaag gaatgggtga
     3121 aggcggtcat aacaggaacg acaactcatt taactaattt gcctcgtact ctcgatcaat
     3181 taacccgtcg ttggtctcag caacaacatg tgattatgac gattagttac gatcaatcat
     3241 tggaggcatc gtctctctat aaaagtggtt ggtacttatt tagatttgcg acggaaaatg
     3301 tgattaatga tgctgttaat aaatatattg atgatagtca ttatattgaa ctgcatagtc
     3361 atcaaacagc gttatgcctc cacataaatc aaactaaagg taaagtaaat gacgagctac
     3421 tcaatcatat taaacatgat atagtgacga aattagacac atattttaaa ttgaaagtac
     3481 gcgttggctt cgggtgtttt aaaaatgaat gggctgatat ggctatttcg ttgaaggaag
     3541 cattaatgga tatagaccag gaagttcaag aggtgacagg cctttataat gaattcatgt
     3601 ttaatgaaga gaccaagctg aattcaaaga gtttatggtt agattctata gaaaatatcc
     3661 accgattatc cgaagctatc cgctacgctg ataaagcgag tgcacaacat gtcattcaag
     3721 cagcgtattt ttattataaa gatcaccctt ttaacatgtc agaagtcatg cgagcaggta
     3781 tcgaaatgtg gacgattatg acgtattccc tctatgatat cggaataaaa attgatgaga
     3841 tgttttcaga tacatttgat tttcattcag gtttgacaaa gctctctcat tggaaagaat
     3901 ttaaaaatta tatgaatgac cttataagtc acatttgtga tcatcaacaa tgggatgaga
     3961 atcttaaaca ccgtcaatta attgaacaga tgctagatta tattcataaa aatatcagca
     4021 aaaatattac attacaagat atcgctaatg aattgtatat ctctcgtaac tatctcggtc
     4081 aaatttttaa aaaagtggtg ggggaatctt ttaaaaatta tttaacgaga gtacgtatgg
     4141 agaaagctaa aaaaatgatt caagagggcg accacttgat ttatgaaatt tccgaaaaag
     4201 taggatttat aaaccctgct tactttacaa cgacgtttaa aaaatatacc ggttacacgc
     4261 cttctgaact tatcaatcga aaattaatgg cagctcatga acaaacagtt caggatgctt
     4321 aattgcttgt tttccctcac atatcacgta agggttttat tttcatctat ctatctaaat
     4381 tgttttctag atatgttatt cacctactta ctttcaccag acgtcaaaat tggaataatg
     4441 aatgtgaact gtatttcaga cgaacccttt cccgggggct tgtcttcagc taacttttcc
     4501 tcaataaatc ttgtttttga aacatcatta taaaatcacc tcaaagtaca aatttaatgc
     4561 caatttcaat ctcgttttct tataaataaa tacccaattt tcaaagagta tgttacaatc
     4621 aaactataat gaactttatc gaacataata aaacataact agggagtgaa aatatgaagg
     4681 agatgttttc attagaaggc aaaacagctg tggtaacggg ggctacaaga ggtttaggac
     4741 aagggatggc tcttggttta gcagaagctg gcgctgatat tatcgggatt agttacttgc
     4801 cacaaaaaga gactcaaaag gaggttgaag cactaggtcg tcaatttttt gatataaatg
     4861 cggatttgtc tattcaagac gagttggctt ctgtagtaaa agatgcgatt tccaaaacag
     4921 gaaaagttga catccttgtt aataatgctg gcattattcg aagagagcag gcagagaatt
     4981 ttagtgatag agattgggat gatgttgtaa atgtcaattt gaacgccgtt tttcgtttgt
     5041 cgagagattt tggtaaacac atgattgaaa acggtttcgg tcgcattgta aatgtggcgt
     5101 caatgctctc ttttcaggga ggtttaaaag taccttcgta ttctgcgaca aaacatggtg
     5161 tggctggctt aaccaaatca tttgccaatg agtggggaag taaaggtatt aatgtgaatg
     5221 ccattgctcc tggatatatg gtcacggata atactgcacc acttagagcg gataaaaatc
     5281 gttctgatta tattacgtct cgcattccaa aaggcgagtg gggaacacca gaagatttaa
     5341 aaggaccagt tgtatttcta gcttctgaag caggtcgtta cgttaatggg catattttat
     5401 gtgtggatgg aggatggatg agctcataaa taacaagaac tctccccttg atttatacct
     5461 cataagagat cacttgctct cttaattgat gattaaggcg atatacactt aatagtggag
     5521 tgatagcaca tctttgttgt tcacttttct ccaaatcatg acgattagtt atgttggttt
     5581 cttgagaggt aagctttgta gcttaaagga aagtccttta ctaggaaaac aatgttttaa
     5641 aaacgagctg ggtgtgattt tagagctaag ctcgtttttg ttatgatttt ttaaatagtt
     5701 aaagattaaa atatttgaaa taaaaatata aatatatatt ttgtgtgaaa aataaataat
     5761 tttattgttt taaaagaaaa acaacttata attaaactat attaaacgac ttgattaggt
     5821 gcttttgctt aagatttaaa tgtgaatacc tgaacataat gacaggaggt tttcataatg
     5881 gggaaagttt ttcacgtgcc tgaaacaaat ttaatgggat gtggttgttt agcagagata
     5941 ggagtctata tatccgaatt gaacgtcaag aaagccttac ttgtaacaga ctctatgctt
     6001 tcgaaaaatg gtgtggttga taaagttatt gacgttttaa aaaaagcgaa catagcttat
     6061 accacatttg accacgttaa acctaatcca actgttcaaa atgtgacaga tggtgtaaga
     6121 cattataaag acgcgaactg tgattttatc atatcagttg gaggaggatc tccacaggat
     6181 gcggcaaaag ggattggctt aatgataacg aacggtgggg atttacggga ttatgaaggc
     6241 gttgggaaaa caacatataa atctgttccc attgtagcaa tcaatacgac agcaggcaca
     6301 tcagcagaat atacgatcaa ttacgttatt acagatgaag agcggcatgt caaaatggtg
     6361 atggtcgata aaaacagcct tgtaaaaatc tcggttaatg atcctgaatt aatggtcagt
     6421 aagccatcta atttaactgc agctacaggg atggatgcgc tcacacatgc tattgaggcg
     6481 attgtaacac caggtgccta tacggttacg gatgctactg cattgtctgc agtggaaatc
     6541 atcttttact atttaccacg ggcagtggca gatggaaaag atatggaggc gcgtgagcaa
     6601 atggtatatg ccatgtttct aacgggtatg gccttcaaca atgccggttt aggctatgtc
     6661 catgctatgt cccatcagct tgggggcgtc tatgatttac cacacggcgt atgtaatgcc
     6721 atgcttctgc cagtggttga gaaggagaat gcaaaacgac atccagataa attcccgcta
     6781 attgcgaaag cagcaggtat taatattgaa ggaaagacgc ctgatcaatg tgcagatgaa
     6841 gtgattaaaa aaataaaagc attatccaaa gtagtcggca ttcctactag attatcagaa
     6901 ttaggaatag atagtgtaga tattaatctt ttggcggaaa atgccatgaa agatgcgtgt
     6961 gcatcaggga atcctttcat ccccacaaga caggaagtca tcgacatgtt taaagagatc
     7021 gtataatgag gaggagcgga ttaagaatga ctgaaacagt taagaagacg atactgagtg
     7081 ctgactttat gcaagacatg ataaaaacga caacaaacct ttataggtta ggctgggatg
     7141 agcgtaatgg tggaaacatc agctatcttt taaaggagga agaagtagct gcttttacag
     7201 atgttaatca aacgattaaa actattccgc taaattttca tatacctgag cttagttatc
     7261 actatttttt agtgacaggg tcgggcaaat attttaaaaa cattgtggat gctcctgaag
     7321 aaaaccttgg tatcattcgt attaatgaag gaggggaaac tgcagctatc atttgggggt
     7381 ttagtcatgg caccttaccc actagtgaat taccgtcaca tttaatggca cataaggaaa
     7441 ggttagcaag ggatgccagt catcgggttg ttatgcatac acatactaca cacttgattg
     7501 ggatgacgtt tacgcatacc ttaatagaaa aggagtttac aagaacacta tggaaaatgt
     7561 gtacagagtg tatcgttgtc tttccagaag gtgtcggtat aattccttgg atgatcccag
     7621 gaacagatga aattggcaaa gccactgctg acaagatgtc tgacgttcgt cttgttgtct
     7681 ggccccacca cggtgtattc gggacgggta atacaatgga tgaagcattc ggattaatag
     7741 agacagctga aaaagcggcg gaaatttatt ctctcgtgga ggggaaaatc aagcaaacaa
     7801 ttacggactt gcaattatac gatttggctc aagcgtttaa tgtaaagcct agagaaggct
     7861 atctaaatga gacttaaatt aacgaccaaa aaagcgcctt attgaaaggc gctttttact
     7921 tttgtagtag atctttttag gttgccatgt tcatcgactt acagtaaccg taaaacggat
     7981 taacgaaaac gctagaaggg gatgagaact accttacatc tatagtatta aatggtattt
     8041 aacaaggaat aaacaattgt ataattaagg acttttgatt ttgtgaactt tttgtgacat
     8101 ctattttcaa catctatgtt actatatacg aatctaaaaa attgaaaata attaggtata
     8161 aaagactaaa taagagaagg ggttcaaaat gaaaagtagt ttagctataa aacttaacgt
     8221 tcttattttt tcaatattaa ttttgttttc catcgtgcta ggtagtgtcc tttacaagca
     8281 aattacagat gggatcaaag aagcagctgt agaaaaagct gcttcagatt tagaattggg
     8341 atttcgttat attgataaag cgatacctgg agagtggtat gtagaagagg gtgttcttta
     8401 taagggtgac acacctatga atgaaaatta tgatcttgtt gatgaaatag gagatatgac
     8461 tggcggtaca gttaccctat ttcttcatga tacacgaata gctacaaatg ttattttgga
     8521 tggtgaacgg gctgtgggga cccaagtttc agaagaagtt gcagaagcag tattgtcacg
     8581 agaagagatt tacttaggtg aagcagatgt agcaggttac agttatcaga cagcttatat
     8641 gccaataaca agcgctaatg gtgaagttgt tggtatttgg tacgtgggtg cttctcagga
     8701 gtttatagat agcacagtta agagtgccat gcagggattt ctaattgttt taactgctgt
     8761 tatggtagtt gcagtaggtg ccgtatttgt attcactaag cgtttaacac gccgtttaag
     8821 taaattaact gacggtgtga aagcggcagg agaaggtgat ttttcaattc aattttctga
     8881 tacgtctaaa gacgaattcg gccaattagc tggaagtttt aatactatgg taaaaaaaat
     8941 tcatagattg ttatcaaatg tgtctcattt gtctcaacaa gtagctgcgt catctgaaca
     9001 gttaacagct agtgctgagg agacaacgaa ggcgacagat cagatctcag aatcggttca
     9061 aaatgtagca gctggtactg aaaagcagtt agagaaagcg gttgtttcta atgaagtagc
     9121 tgaaacaatc tctttcgaaa tggagaatat ctctcatcat atagaacagg caaatgaaaa
     9181 agcaaatagg acttccaaaa ctgctgaaga aggaggtaag gttgtcgatc aaacgttgat
     9241 gaaaatgaaa gacattcaag acaagtcgga gagtggcgta caagttattc gtagtcttag
     9301 taataagtct gttaaaatag gggacatttt gtccgtcatt tcaggaatct ctgaacaaac
     9361 gaatctttta gcattaaatg ctgcaattga ggcagcgagg gctggcgagc aagggaaagg
     9421 atttgcagtt gttgctgaag aagttagaaa gttagctgag gaatctgggg attctgctaa
     9481 acaaattagt acgatgattg gtgagatcca agaaggcatt agtgaatctg tgacagcgat
     9541 ggaaagtagt cgcacgtctg ttgcatcagg aattgagaat gctgataaag caaaagagac
     9601 ttttaaagat atcagtgagg cgattaagga tgtttccgat caagtcatga atgtgactga
     9661 agcaactcag cacgtgacaa caaatatagg gaaaatggtt acctcaataa atgaaagtaa
     9721 gcttattacg gaaaatgccg ctggttattc acagaatgtg gctgcttcta ccgaagaaca
     9781 gacagctact atggaggaaa ttgcatcatc tgctttcacg ttagctaaaa tggctgaaga
     9841 tttacaaaaa gcggtaagtg cttttaagtt ataagaggat gaaataaaga gagttttttt
     9901 aaatagtatg tttggaagtg gcacattact taagggcaac cgtctatttg agacgttgcc
     9961 cttaaaaaat acatatattc agaacaatag tatatctaaa ttaatgataa tttttaatat
    10021 cttcaaccgt ttgattcact tcgtctctag acattttttc acgaccagac tttagaacat
    10081 gaagtgttct cttggctaat tctagcggga ttttgcaaaa gagactaaca tttttactag
    10141 tgatgtcttt catatattca tctccgagag caaggttggc ctcagcgtac tgaaacgtat
    10201 cagcaatatt ccaattttta ggtatgaatg aaattcctcg atctttatct tcttcttgat
    10261 tacgaagaat attgactgtc tgtaggccgc gtccgtaagc aatagcatag tctctgtccg
    10321 ttttcgttcc atctcgccat tcccaaatat cagaaagcat aacacctaca agaccggcta
    10381 cataataggt gtaatcgtct aaatcttctt tcgtttgaat atcccaattt ttttcggccc
    10441 attttgccat accttgagcc ataatacttg tagattcctg cactttttct ataatatccg
    10501 cagggcaaac tgaaagccaa tcgccgagtc ttattgtcac ttcagggagt agctgtttgt
    10561 agggctcaat caattcaagg tatgcgtcat tgttaaaagt gtcgcttaat aaaacacttg
    10621 ttttcgagag aagatgagat ttcgttgcag gttcaagatt ctcatggtct tcaatttcgt
    10681 caattgccct catacataag taggcggaag ccactgtttt ttttaatgtg ggctctaata
    10741 atgtgattgg aatataaaag gttctacttg ttttttttaa ttgtcccata gcctctttat
    10801 gtaactgttt ctcattcttc atatcatttc ctccttgatt ttaccccaaa gcttatatca
    10861 tcactaaaag gacatcgcac ttaaatttaa caaacgcgat atagtgaaaa gtgtttagaa
    10921 atatgtcgag taccaagtat catttactct aaaatactac aaaagcttat cattattcaa
    10981 cttttttaag aagagacagg ataaagactt ttatctttct tttatatttt ttaagaaatg
    11041 aacgcctttc attatacaaa ctaccgctgt aacaaacgcc tcatacatta atcttctact
    11101 gtaggcgggc gctttctcga ggtttgtttt ttactacagc ctacttttta aaaacgagca
    11161 ggtattattg tcccttggtc gtgttaaacc ttcaatatgt aacagtcaat agtataccac
    11221 cttctggcac taattcatta ttttaattct aaaggtggaa gtgtttacat gtcactatga
    11281 aagggaacag atcagtaaaa cttgaaagga gatttacgta tgagtcaaaa gattcaagcg
    11341 tatttcaaaa ctgaaaatga tgctgaaaaa gcagtagcac acttacaaaa attaaacacg
    11401 tctaatgtaa tagttgatga acttcctgaa ggtggaagat cgttgattct tttccctgtt
    11461 gctaatctgg gtaccacggg aagtgggatg gggaactttg gagctcctgg cttttttgga
    11521 agtaaggaag aaataaaaag tatgggatca aataaaagcg agacgtttac tcacattgtg
    11581 gaatttaata tatcggaaga tgaggctaaa gaagcgtttg ctacgcttaa ggaagcggat
    11641 gcatatattg attctgagct gaacagttct ccctaaactt gaacctaccc ttccacattt
    11701 aagccgacta aatgtgttag aaaaagttga taggttatat aggctcgcct attggcgggc
    11761 tttcttaaat gtgtgtgtgc aagagcttga gagaaatact cgcctgtatg tatgaaaaag
    11821 atatagtaaa tttagaatgt tttcttttca tagaaacttt ttcataaata tattcgtaat
    11881 gatagaaggg agggaacgat gaatgatgac atggataatt agccttctta ttctatgcag
    11941 tctcattgct ggatgcagtg aagtagtaga accaataagt gacattgaaa aacttcgtga
    12001 tattacgatc gaagaacgtt tttctgaatc cgtgaggagg gatgagatag aaaaatactt
    12061 acataaagag aatggtgata acgagattga gtcagatgat ttaaatatgc taaaagtgtc
    12121 taatgggaga gcggagacag tcacaaaaga ggaaatgtta ctggatagtc atctgttaaa
    12181 tcaaatatgg tctcattctt atgcattata tgaggtgtat ggtggtcaag atagttttag
    12241 tcaagcacat gagttattga cagcgactat tgaagacgag tattccgaag gggaggaggc
    12301 taattacgag gagttcattg cacttattag tgatcattat gcttttataa cggatcaaca
    12361 gttattaatt gatggttccc cattttttga ccgtgaacat cagttttata ttactgaaga
    12421 actgactttt gtgaaagatg ataataataa ttattggctt tataaagata atcataaaaa
    12481 agacaaggtg gtctctatca atggagacga tcctgatgtg tatttgctcc caagtcttaa
    12541 ctttgaaggg gaactagttt attttccagg gatgtataca gattatttgc cgcaaaaatt
    12601 atgggatatt gatttggaga atcaagagac tatagaggtg accgtacgtc aatctggatt
    12661 agaccgttta agagacacat cgtttgacgt ttatgaagac gaagaggtac caattattca
    12721 gctgagaaat atgttagttt acaaggaaga ttcatataat tatgaagata tgttgggctc
    12781 agtggatcag gtagaggatg aatcgacttt tatccttgat ttacgaaata ataatcaagg
    12841 gcacattaag tttgcccagg aatggtataa gagattgaca tcacatgaac cgagtataaa
    12901 aagttattct gctcaattaa tgacgcctac taccattacc tttttagagg ataaggtaga
    12961 aagaaacaga gaagcggggg ttgaatgggg aggtttattg gatactgaag atccttttaa
    13021 cattaacgag tatcatgatg tgactcggtt gcagtggtcg attgaagaaa tggagacaga
    13081 tggcataact gagcttgatt ccactattta tattttaatt gatgaagcga cgagtgcatc
    13141 tgcagaaata ctagtggctt acttacgaca gcatcctaat gtgtatattg taggtgcacc
    13201 tagtgaaggt gccatgagtt caggaaacag tcaattaata gaacttcccc actcaggggc
    13261 aaaagtgagt atacctagta tgttccgcta tcatccacaa tttttagaaa aagaggccat
    13321 tggtatagag ccagatttat gggtttatcc ccgttttgcg agagaacgtg tcgtgaagtg
    13381 gatagataat aataatgacg atttataaac ttttaaatta agttaaagag ttttttattg
    13441 cacagataag ggtttgtaaa acctctcgcc acaaataaag aggagagcta actctattaa
    13501 ggagggaaaa aacgaccgct aatatcctaa gtaaagcaac tcccactgat tgaagggccg
    13561 ttttatagag gcgcgtatag atgacgtaaa aatgaagaac gatccttttg ttcgctcatt
    13621 agagggactc aaaaagggag gccctcaatt gtctttgggg cgtcccttag ttactgctta
    13681 ctgaaattga ggtagatatt ctttgtgcgc ttcaagcatt tcatcgagta tgagtttagc
    13741 ggttgtatca gacgggacaa gtggattaat tgtcattgct aacaaggctt gatcgtagtc
    13801 accactaacc gccgcttcag cagcaatgcg ttcgaaagat ttaatttgct gtacgagccc
    13861 tcttacagag acggggagat cgccaactgc taacggttta ggaccatctt ttgtaatgat
    13921 gcagtttact tctacagcgg agtcgtccgg tatactgcca atcgcccctt tgtttaacgt
    13981 attgacaggt tgaatatcac cacgatcatt ataaatggaa tcgataagat tacaagctgc
    14041 atcactataa taagcaccac cgcgtttttc tagttgttca ggtttggtac gcaaggtttc
    14101 atctttatat agttcaaata actctttttc caattgttga acaacttcag cccgagttcc
    14161 ttcttcggct gcgtttttct tctcttctgc caacatatca gacgttttat agtaataacg
    14221 atggtatgga caaggaagca tatttaatgc tttcaaaaat cctggttccc acccgatgtc
    14281 tacgatgttt ttcatcgtca ttgaagaatc tcctgacgtt attttattaa taacgtcttc
    14341 tgtgacacac ttgccgtcta aataaacgtc catgccgtat accatatggt ttaatccagc
    14401 aaaatcaacg tgtattctat tggcttccac atcaagagct ttggctacac ccattttaat
    14461 ccctacaggc acgttgcaga gcccgacgac tttgcgccag ttagagtgtc tataaatagc
    14521 ttctgttacc ataccagcag gatttgtgaa attaattaac catgcatggg ggcatagacg
    14581 ttccatatct tgaataatat caaggatcac tgggatagtt cttaaccctt taaaaagccc
    14641 tcctggacca tttgtttcct gcccgattac atcgtacttc aaaggaattc gttcgtcttt
    14701 agcacgcgca tcgagtagac caacacgaaa ttgagttgtg acgaaatcgg catcttttag
    14761 ggccttttca cggtcaagtg tgagatgaat tgtcatggga acaccagctt cttctaccat
    14821 tcgtttagca aggttcccta cgattgtgag tttttcttct ccttctggaa tatcaagtaa
    14881 ccataactca ctgatcggta atgagtcata tcgtttaata aatccttcga ctaactctgg
    14941 tgtatagcta gaaccgccac caatagttgc aatttttaat ccttttttaa tcatgtttaa
    15001 cactccttta atattataaa tataagataa gatggacgaa aaccatttca tttgtataat
    15061 agaaagtgaa ataaacgtat gagcgtttta tactatttga aggcgcttaa ataatgacaa
    15121 tatggcgtaa gccaatgtaa aaaggacgat gctaatgttt acaccgaaac agcttgcccg
    15181 cttttctgac ttagacacac ttctatatca ctacatacta gctcacggag aaaaagttgc
    15241 ttatatgcgg attagagaac tggcggaagt aacacatgtt tctccaacga ctattttacg
    15301 cttctgtaag aaacttgatt gtgacggttt ttcagaattt aaaactaagt taaagcttta
    15361 tttagagaaa gcggaaacga aatggttaaa tcatagttat gaatcaatga atgaattttt
    15421 ggaacggaca ttgaaatcag attatcaagc tcaattaagt gccatcgcag acgagattgt
    15481 atcttcctca caagttgtct ttatcggaag tggtagttca ggcatcctag cagaatatgg
    15541 tgctcgttat ttttcaagct taaatacttt ttcatcagcc ataagtgacc cattttatcc
    15601 gattaacgga aagcaaatac gtcatagtct tgctattatt ttatctgtat ctggtgagac
    15661 acaacacatt gtttctaagc tacatcgctt tcgagaagaa ggtgccacga ttgtaagtat
    15721 taccaataca aaaagtaaca cagtagcaca gttggctgac tacccattag cgtattatat
    15781 taatactgaa tttaatgata tttccaatgt gacgactcaa cttcctgttc tctatttaat
    15841 agaaacgctg gggaaaatag tgtttgaaaa gttataactc attgtgatgc gtagatgcct
    15901 tattttaggg taatgcgccc ttttaaagct ttcagtcacg ttgaaggtat gctttgtaat
    15961 gataataacg taatgccttt aaagcgggtt ttctagagag aaactctcaa tttaaaattt
    16021 gtgtcggccc tcctttgtgg tcttacacta tttattatta ctgtcttgct ttgttcagta
    16081 aagagtatat aggcaatgag gaaaacgtct atataaaaag aaacgtgttc caacagagga
    16141 acacgctgta ccatattgaa cgttttatgg gttaagtagg ttgattagaa agtgtactcg
    16201 ttaaatctct atattcttta ataatattat caataatgtt ctttactggg aggatatcat
    16261 tgatttcatg cacacgagac ccactaaaga caaggccttc ttctgcatcg cctgcacggg
    16321 attttaacaa agaatctaaa atacaaaagc gatgtgagca tttctttaaa catgacatac
    16381 atttttcaat ttttactttt tttccatcac ttattttgtt tgtgaacgta tttttaatgg
    16441 cacgtccttc taacccgacg acagatgtta ttaaagtggt atctcctttt ttactagaga
    16501 gatacttttc tttgaaagat tggggggcat cacattcagt gctagcaaca aatcgagagc
    16561 ccatttggac accagaagcg cccaaagata acgctttttt aacatcgttg ccattgataa
    16621 tgccaccagc agcaatcact ggaatatcaa ctgattccac gacttcaggt aatatatcga
    16681 aaagtggtct atctgtccct aaatgtcctc cggcttcaaa tccttcgacg acgacagctg
    16741 atgctcctaa tcgttgagac atttttgcta attttccaga agaaacaata gagatgacag
    16801 gcgtgttgta ttctttcccc caagcataca tatctctaga tataccagca cctgaaataa
    16861 taaagtccac tttttcttcc agagccgcct tcattgtgtc agcaaaatct gtcatggcat
    16921 atagaacatt tacacctata tagccaatac cttttgttag tttgcgagct cttctaatgt
    16981 ggcctttcat ttcctctatt gggatacctg ttccggaaat gatccccatc cccccggcat
    17041 ttgcaacggc tgctgctaaa ttgctaagag atatacctac acccattcct ccttgaatga
    17101 tcggtacttt aggcttcatc tgtgcaattt ttagttgtgg taattccatt caactcaccc
    17161 tcttattgtg tcagtagagg tcaatggtaa gaatatgttt acttaacatg acctatacag
    17221 atcgtatcaa cacaaaagtg tcatgacaat aaaaattacc accagcctat gataattggt
    17281 tttaattatg aaaattttat gacaattcga aagtgacctt tttatgatta agagtaagga
    17341 ttcatgtggg gcatagtgtt agaacgaggg aggtgcttca tcaatttgtc gatatgacat
    17401 ttgtcatatc atttatctga cgcttgtgac ttatatggga aacagattgt aggtatactt
    17461 ataaaagtga caaaaatgtg tcctttaaaa ttgttcagga ggcgagtgcc ttgagtaaag
    17521 aaagtattgt taagttaaag aaagagatgg cacctttcga aaaaacagaa cgaaagataa
    17581 gtattaaaca gatgattaac acgttagggc ctcttgtgat tttatggtac ttggcttatt
    17641 taagtcttga agtgtcttat tggatgacaa tccctttact tattacggca gcgggttttt
    17701 tagttagaac ctttattatt tttcacgact gctgtcacca atcttttttc aaaagtcgta
    17761 aagcgaatga tatacttgga acaatcacag ggattctaac cctggttcct taccaacaat
    17821 ggaaaaatag tcataatatt catcatgcaa caagcagtaa cctcgataaa agaggaacag
    17881 gagatatgtg gctcttaacg atagaagaat atgcagcagc ctccatctgg cgaaaaatag
    17941 cttatcgcct ttatagaaat cctgttgtta tgctaggact tggcccactt gcagtctttt
    18001 gtattgatta tcgttttaat acaaagggcg cgaaacgaaa agaaagactt aatacgtatg
    18061 tgacaaacgt gtctatcgta gctttgtacg gagcattaat ttgggctatc ggttggcagt
    18121 cttttttact aattcaagga ccgatcctac tcgtttctgg catgttaggt atttggttat
    18181 tttacgtcca acatcagttt gaagatacgt attttgaaca tgatgacgaa tggagttacg
    18241 tgcaggcagc tgtggaagga agctcttact ataagcttcc aaaggtgttg caatggatta
    18301 caggtaacat tggttttcat catgtccatc acttaagtcc gagagttcct aactattatt
    18361 tggagaaagc gcacgaagcc acaccacctc tccaaaaagc aacgacaata acgattgcaa
    18421 cgagcttcaa atcattacgc tatcgtcttt gggatgaaac gacaaaatct tttgttacat
    18481 ttaaagaagc taagaagtta atgatgaaga aaacgcactt agcataggtg aaatgagtgg
    18541 taatatgggg aaaacatgcc tatataacgc ctcatataag ctataatata gaggaacaga
    18601 agacgctata aatggaaaag aggtgggatg atgaaaaatt ggacgaaaaa atttcgaaaa
    18661 aacacggggc ttagtcctta tgtttgggtc gttttttaca tcctgccctt ttatttcatt
    18721 ttacaagatt cctccacgtt ggaaacgatg attggtattg tgatgaccat tgtttttttc
    18781 ctatgttatg tcttatcgtt tgcttctcat ggatggcttg tttatttggg agccagcatg
    18841 caaattgcca tttcaactgc catgagtttg ttgttcggtt acatttattt ttttctcttt
    18901 ctagcctttt ttatcggcaa ccttaaaaat agagttgcct tttttacgtt atacattatt
    18961 ttactcatta caaccttcgg aacgagttac tatggattcc ttatgcatag tgtttttatt
    19021 acacagcttc cgttcgtctt tttaagcatt attgctgtta tccttctacc ggttaatacg
    19081 tataacaaaa acaaagagga acagcttcaa ggacaactta atgatgccaa taaacgtatc
    19141 gcagagttag tgaaattaga ggagcggcaa cgtatatcgc gagaccttca tgataccttg
    19201 ggacagaagc tttctctgat tggtttaaaa agtgatttag cggcaaaatt agttgaaaaa
    19261 aatccctcaa aagcacagct tgagattaaa gatgttcagc agacagctag gatcgcctta
    19321 aaagaagtgc gagaattggt tatggaaatg cgagggacaa aactaaatga tgaaatcaat
    19381 cgggttgaac aaatattgca agcagcaacg attaaatttc atcttgaaaa gccagacgaa
    19441 ctaccacgta ttccactaat cattgaaaat gttgtgagca tgtgtttaaa agaagcagtc
    19501 acgaacgtcg ttaaacacag tcaagcatcc aagtgcgcta tcataataaa agctttgaaa
    19561 acagaattaa ctattactat ttctgataat ggcattggga taggaaaatc tgaacgtaat
    19621 aaaaatagtg gcttacaagg gatgaaagaa aggctggaat ttgtgaacgg gcatttgaaa
    19681 atagaggata ctaacgggac caatgtcatt attaatgtac ctgtaataag caatcgaaag
    19741 aggtgaatca gttgattcga attattattg ccgaagatca gaaaatgtta ctcggcgctc
    19801 ttgctgcttt gctggattta gaagaggata tgacggttgt tggtagtgcg agtaatggaa
    19861 aagatgctat agagctagtt tataaacatc aaccagatgt atgcattatg gatattgaaa
    19921 tgccaataaa aacaggactg gatgctgccg aagagctgaa attgaaagat atagcgtgta
    19981 aagtcatcat tttaacaact tttgcccgaa caggttattt tcagcgtgca gtaaaggcgg
    20041 gagtcgatgg ctacatgtta aaagatagcc cgagtgaaga gcttgtgaat gcgatcagga
    20101 gcgtcatgtc aggaagacgc atttatgcac cagaactagt ggacgaattt tatagtacgc
    20161 cgaatcctct tactgaacgt gaaaacgatg tacttcggtt aatggctgac ggaaaaagta
    20221 caaaagaaat tgcagattta ctacatttaa caaatggtac aattcgaaac tatatctctt
    20281 ccattcttga gaaacttgaa gtcagtaatc gtattgaagc gattacgcgc tttaaagaaa
    20341 aaggttggtt taaatgattc tgtcataaca aatgttgata tgttcactag acatttactc
    20401 gcatctaggc aaaggtatta ctggatataa tcttgtgatg tctttctcgt cctcaggaat
    20461 tttatgttga aaaagcagcg acatcacgat agaaacggtc agccagtatc ggattcaagt
    20521 catttttttt gaattatcga gcgcataaat aatcacatca taacgcagtt taattgtatt
    20581 taatggtaag tgtaatggtg aaatacttgt aatgttctgg aaagaatgta ttataaaata
    20641 aaaaagctcc gccattggcg aagctttaca cgctatttta aaataaaccg atggagctta
    20701 agaaaataat taagatggcg attggcgcaa tcgttcgtaa aataaatatc cacgtatgtc
    20761 caatccatgt atctccaaaa tctgatgctt ccatagcctc tgctttgttc cagcctatcc
    20821 cc
//
