LOCUS       NZ_KV917378            22126 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Salipaludibacillus agaradhaerens strain DSM 8721 Scaffold1, whole
            genome shotgun sequence.
ACCESSION   NZ_KV917378
VERSION     NZ_KV917378.1
KEYWORDS    WGS; RefSeq.
SOURCE      Salipaludibacillus agaradhaerens
  ORGANISM  Salipaludibacillus agaradhaerens
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae.
REFERENCE   1  (bases 1 to 4319439)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Genome sequencing project for genomic taxonomy and phylogenomics of
            Bacillus-like bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319439)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2017) Agricultural Biological Resources Research
            Institute, Fujian Academy of Agricultural Sciences, No. 247, Wusi
            Road, Fuzhou, Fujian 350003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 227.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002019735.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 15:23:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,010
            CDSs (total)                      :: 3,909
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 101
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 17 of 38
            Pseudo Genes (incomplete)         :: 21 of 38
            Pseudo Genes (internal stop)      :: 7 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:59:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2528638
            Orig. end                         :: 2550764
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV917378.1.
            Bacteria and source DNA available from DNA is available from: DSM
            or Fujian Academy of Agricultural Sciences.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22126
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="opine-like-metallophore"
                     /region_number="6"
                     /rules="(CntL and CntM)"
                     /tool="antismash"
     cand_cluster    1..22126
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(CntL and CntM)"
                     /kind="single"
                     /product="opine-like-metallophore"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22126
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:12126](-)"
                     /cutoff="5000"
                     /detection_rule="(CntL and CntM)"
                     /neighbourhood="10000"
                     /product="opine-like-metallophore"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12126)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(CntL and CntM)"
                     /neighbourhood="10000"
                     /product="opine-like-metallophore"
                     /protocluster_number="1"
     misc_feature    complement(531..540)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    complement(1608..1620)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.29"
                     /tool="antismash"
     gene            complement(3025..4209)
                     /gene="cntE"
                     /locus_tag="BK581_RS11780"
     CDS             complement(3025..4209)
                     /codon_start=1
                     /gene="cntE"
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636411.1"
                     /locus_tag="BK581_RS11780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="staphylopine family metallophore export MFS
                     transporter CntE"
                     /protein_id="WP_078578366.1"
                     /transl_table=11
                     /translation="MNGAMSWPFLRLYLLVLFYFSANAILNVFIPLRGEALGATNTTIG
                     IIMGAYLFTTMFFRPWAGQIIQKHGPLKVLRFILVFNGLALILYTITGLGGYLIARMMQ
                     GVSTAFFSMALQIGIIDALPEEDRSQGISLYSLFSYIPGIIGPLLAIGIWQGGMGYFAI
                     VMVTIAMLTGLFGFSANVDTPKTKPMTDQAHSSGSIVQSLSQLVKNPDLCRSSAFMLVG
                     SLIFGATTIFIPLYAVQVDKGNAGIFLMIMAATVVFARFTLRKRIPSDGKWHAPFMIGT
                     MLLLALGSQSVSIAIYGGAFFFYIGAVLIGTAQALLYPTLTTYLSFVLPEASRNVLLGL
                     FIAMADLGVSLGGIIMGPVADMLSYSRMYMVCAILSVAILGVVVYDRKKIVVQE"
     gene            complement(4467..5210)
                     /locus_tag="BK581_RS11785"
     CDS             complement(4467..5210)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735103.1"
                     /locus_tag="BK581_RS11785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_078578367.1"
                     /transl_table=11
                     /translation="MLKVSHVSKSYKKGGMFSSKKQKVLHNVCFDCQAGECLGIIGESG
                     SGKSTLGRMLLGVEKPDQGNITLNGQIVTNRQVRLGCMSAVFQNYQSSINPFFTVEDAI
                     MEPLMMSKNKINTRQKVDQLLHDVGLSLSYKTTYPHELSGGEVQRVCIARAIATEPEWL
                     ILDEAISSLDMSIQLQILELLKGLKKTYKMSYIFITHDIQAAAYMCDRVLIFKEGQVEE
                     IIAIDQLKYVTSPYAKRLLKALITF"
     gene            complement(5197..6024)
                     /gene="cntD"
                     /locus_tag="BK581_RS11790"
     CDS             complement(5197..6024)
                     /codon_start=1
                     /gene="cntD"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721798.1"
                     /locus_tag="BK581_RS11790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="staphylopine uptake ABC transporter ATP-binding
                     protein CntD"
                     /protein_id="WP_078579926.1"
                     /transl_table=11
                     /translation="MTTLLNVNNLKVWNSHTGQAIIHNSSFDLKQGSCLAIVGESGSGK
                     SVTCRALMKLNKVPLRQSGEILFNGHNVNTLAEKKMRRLRGKHLYMILQNGMGAFDPSC
                     VIGRYVKGILTQHFRWSIKEMEERMMKAMESVMLKNPAHIMTQYPHQLSGGMLQRMMIA
                     LAIVIEPDIIFADEPTTALDTISQFEVVEQLMALREKMGCSMIVISHDLAIVKKLADNV
                     LVMRNGEIVERGQTQAVFSDPEHAYTRYLVSTRLALSDHFKKIMEGDAIVAEG"
     gene            complement(6027..6920)
                     /gene="cntC"
                     /gene_synonym="opp1C"
                     /locus_tag="BK581_RS11795"
     CDS             complement(6027..6920)
                     /codon_start=1
                     /gene="cntC"
                     /gene_functions="transport (smcogs) SMCOG1085:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /gene_synonym="opp1C"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915622.1"
                     /locus_tag="BK581_RS11795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="staphylopine uptake ABC transporter permease
                     subunit CntC"
                     /protein_id="WP_078578368.1"
                     /transl_table=11
                     /translation="MKLLKQLGQDKLAAISLIIIVITVVVGIFAPLCAPHPPNDVNMAL
                     RHASPSWSYLLGNDHLGRCVLSRLIYGIRPSVLAVLVALIVSVLIGALLGFLAGYFRGK
                     VDAVIMRCCDVMLSFPGYVMTLAFVGIFGVGLENILIAFILVKWVWFARVIRTSVMQYA
                     ELEYVTFSKAIGMRHVTIMYKHLLPVTFLDIAVISSSAIGSMILQISGFSFLGLGIQAP
                     NAEWGMMLNEARDIMFTQPQVMLAPGLAIIIVVSAFNFLSDALQVALDPKLINAKQRKV
                     GGKPLSKVVIKEKEVI"
     gene            complement(6921..7853)
                     /gene="opp1B"
                     /locus_tag="BK581_RS11800"
     CDS             complement(6921..7853)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="opp1B"
                     /gene_functions="transport (smcogs) SMCOG1118:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014650326.1"
                     /locus_tag="BK581_RS11800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="WP_078578369.1"
                     /transl_table=11
                     /translation="MGRYIVKRVVIAIPMLLIISFLTFLFINLAPLDPAEVVLKAQGVP
                     TITDELMAQTKEELGMDRLFIVQYFNWLIASLQLDFGSSYVTGKPVWSLLGPAFLNTFK
                     LTIVSSFFIIILSILLGVICALQEGRVMDKSIRGVSFFLTAIPSYWLASMMIWFVSVKL
                     DLLPTNGMTSYQSYILPVTVIVISYAGIYFRNVRTSMISNLNEDYVLYGRACGLPEKTI
                     TIHILRNSLQVAMSIFGMAVPIILGSTVVIENVFAWPGLGSLTVRAILSRDFPIIQAYV
                     LILAVAFIAFNTLSDIITAAMNPKIRNDL"
     gene            complement(8046..9647)
                     /gene="cntA"
                     /locus_tag="BK581_RS11805"
     CDS             complement(8046..9647)
                     /codon_start=1
                     /gene="cntA"
                     /gene_functions="transport (smcogs) SMCOG1068:
                     extracellular solute-binding protein family 5"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016098175.1"
                     /locus_tag="BK581_RS11805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding lipoprotein"
                     /protein_id="WP_245829213.1"
                     /transl_table=11
                     /translation="MMFVSFIVLIGRVLIACAGGEESDADIEASTQAQEEIVYATAQDI
                     NDMNPHLYTGSMPAQGMIYESLVENTEEGIKPLLAESWEISDDGTVYTFHLREDVTFHD
                     GEPFNAEAVKKNIEAVQANAETHAWIKLSTQIEDCRVVDEYTVELTLSEAYYPTLVELS
                     MTRPYVFISPNDFIDGETKEGVSGFHGTGPYMLTDHKVDEYATFTAHDAYWGGEPAIET
                     ITSKVLPAGETTFLALQKGEVNFVFTDDRGVDTLDVEAIDQLVASGAYQLIRSDAMNTK
                     MIVANSSQDESPVSERAVREAIWHAIDRETIAEEILDGSETVADTLFSSNIMYADVDLD
                     VRSFDLEKATDILDEAGWLLEDGSNVRMKDGNTLTMTLYYDSHSSSQKTQAEFIQATLI
                     EAGVQLEIVGEESTSIANRRSTGEYELLFNQTWGLAYDPQSTIAAFTSEASYLHTTSGI
                     AEADTLYQKIEEVMVTTDEDMRQNLYGDILTIVHDEAVFIPISNGSLTVVAPAELEGVS
                     FKQTQFELPFEHMSFK"
     gene            complement(10001..11302)
                     /locus_tag="BK581_RS11810"
     CDS             complement(10001..11302)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     opine-like-metallophore: CntM"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006210265.1"
                     /locus_tag="BK581_RS11810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="opine metallophore biosynthesis dehydrogenase"
                     /protein_id="WP_078578370.1"
                     /sec_met_domain="CntM (E-value: 2.1e-181, bitscore: 601.2,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAFKRVLLLGTGPVAVQLAVIIKRHLTCEIGIAGRKSVRSDAFF
                     DALTDQHQRLDVTTQNSKNDNLAGDCAVDEVFTSYETVKGDWDTLILTVTTDAYLDVLK
                     QLDKTVLEGVKCVVLISPTFGSNRLVDAYMSAFDDTIEVISFSTYLGDTRWRDDTPSNR
                     VLTTAVKHNLSIGSTHGKSENVNTLCDLYDTLGVNVNVMRSPIEAESRNISLYVHPPLF
                     MNDFSLRHIFEEQETKTYVYKMFPEGPITYALIRHMLAQWKEIMGILEALNIDGINLLK
                     FMTEDNYPVRPESLPREDIENFNQLAPIHQEYLVYIRYTSLLIDPFSEPDKDGRYFDFS
                     AVPIRPVFVNREGYLDIPRMPKEDYYRLKIIQGIAHHLQVKSDTIDHFIQTYEEAIRNA
                     AQSHDGKPLSDAFRVQTFHDDVAMIVRNLKSVTS"
     gene            complement(11332..12126)
                     /locus_tag="BK581_RS11815"
     CDS             complement(11332..12126)
                     /GO_function="GO:0030410 - nicotianamine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030418 - nicotianamine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     opine-like-metallophore: CntL"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006638079.1"
                     /locus_tag="BK581_RS11815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nicotianamine synthase family protein"
                     /protein_id="WP_078578371.1"
                     /sec_met_domain="CntL (E-value: 8.7e-98, bitscore: 324.8,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTLTTFQGYLSRLLTKFDDLAGNYDDTMTYSPELERLIDEYSTF
                     ITHEANKNVWEQLIHEDNAQLAQLVVNLRRQSARCVAIMEKYRALKLLNHHESMTDYFK
                     NIESSIESEFGSFHVTAKSKVLLVGSGAFPMTLLLIAKRTGADVIGIDIDDEAVQLGRE
                     VVEKLGKGLNVRLEQSPIENIDKLKTVSHVIFSSTVEQKYDLLDHLHPLTRNDVVVAMR
                     YGDQLKSLFNYPSREVDKHKWRKVNTIVRPEHVFDIILYQKA"
     gene            complement(12149..12982)
                     /locus_tag="BK581_RS11820"
     CDS             complement(12149..12982)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676614.1"
                     /locus_tag="BK581_RS11820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_078578372.1"
                     /transl_table=11
                     /translation="MKREIDFVKFNPTQNMTILIDDSNVVQDYEKIATKIMAYENVYGE
                     QVGFIKTPLNKKASALLRMAGGEFCGNACMALGTLKAVEQGLELNETLDIVLEVTGTDH
                     LITCQVRRTPHKYYCQLNMPIPTTLEQTTLHYEGSELSVTFVTYREAIHLIIEADTFND
                     SLKDKAESLARLLGGTLNTHLIGILMYSPISNELAPLIYVPHLDSMIWERGCGSGTASI
                     GAYLTWKNKRKTVADISQPGGSITVSGDYIAGEIRDLNIEGSVELVAKGTAYINL"
     misc_feature    complement(13501..13511)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            complement(13540..13752)
                     /locus_tag="BK581_RS11825"
     CDS             complement(13540..13752)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010096922.1"
                     /locus_tag="BK581_RS11825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_245829016.1"
                     /transl_table=11
                     /translation="MVLFLTVSFIIIGFVVVISMKGKMERKLAFIQENADNHKNANTPI
                     ILWIVGTTFWGIVSMALIIGIFFMN"
     gene            complement(13937..14560)
                     /locus_tag="BK581_RS11830"
     CDS             complement(13937..14560)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003323752.1"
                     /locus_tag="BK581_RS11830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_078578374.1"
                     /transl_table=11
                     /translation="MLGAILHGMILSFGLILPLGPQNIFLFNQGAAHSKVKFALPAVIT
                     ASLCDTLLIILAVLGVSVIVMTIPAFQLIFFAIGFFFLIYIGWSIWNSNTSEVNKKHAA
                     MTGKKQAMFAISVSILNPHAVLDTVGVIGTSSIRYTAIPEKIAFAAACILVSWVSFFAL
                     AYIGKTIRSIDKEGKIVRMINKVSAIMIWGVSLFIGYQLYKIIF"
     gene            complement(14585..15589)
                     /locus_tag="BK581_RS11835"
     CDS             complement(14585..15589)
                     /EC_number="1.14.19.-"
                     /GO_process="GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) delta12_cd03507"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486715.1"
                     /locus_tag="BK581_RS11835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="fatty acid desaturase"
                     /protein_id="WP_407690336.1"
                     /sec_met_domain="delta12_cd03507 (E-value: 2.7e-11,
                     bitscore: 40.8, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVMTSEDKKLKKATFSKEIVNQVRALQTRNNYTNLFSILFDWLV
                     IIASAYGAILLSNWWSYLIAITIIGSRMRAFDNLMHEASHVLLFKNKIMNKWITCFFVA
                     FPVFTSFTAYCNSHHAHHRHLWNIDKDPDTQRYSLIGLDKPQKSIKTFIFKHIIKPLTL
                     THVPKYIYGTLKVNVMSKEEPLSEKVAKPIFWCIIITCSIIFNFWLHIILFWFVPLLTT
                     FQVIRYWAEMAEHSGLKNDHELYASRNTFGNPIQIFLLHPHHDNYHLVHHLFPAVPHYN
                     LKKVHLILMEDYDYKKAHHCTGFFKTFLPGFKSVIEDICSDATKKISRYQRQK"
     gene            16838..17620
                     /locus_tag="BK581_RS11845"
     CDS             16838..17620
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228843.1"
                     /locus_tag="BK581_RS11845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_078578376.1"
                     /transl_table=11
                     /translation="MGINFHSDKNSTTYTTRKADKTWVEAIRKLVPIEEVSYAADIGCG
                     GGIYSKALSDMGVDEVTALDFSKSILNGAKENCKDYQTISFKHGNAFETGLASHHYELI
                     LERALIHHIEDLNACFNEAFRLLKDGGYYILQDRTPEDCLVKGEDTHIRGYFFELFPKL
                     VEVETSRRHHSQHVIDMLKKVGFKDVEEVKLWETRHVYHTKEPLLNDLYERTGRSILHE
                     LEDDELHLVIDHIDQSLPKNTPIVEKDKWTIWKAVKSV"
     misc_feature    complement(16849..16863)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.15"
                     /tool="antismash"
     gene            complement(17699..18013)
                     /locus_tag="BK581_RS11850"
     CDS             complement(17699..18013)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK581_RS11850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_078578377.1"
                     /transl_table=11
                     /translation="MKRKFRIIISIILMIGIGISGYIFYAKNKLESDVIEHVMVEMKIP
                     TESIESSEAFIANLPGLRNYMVSIKIENDTNTYFYYKDKNDDIILESYTDENGVEYIIE
                     "
     misc_feature    18150..18165
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 23.04"
                     /tool="antismash"
     gene            complement(18204..18551)
                     /locus_tag="BK581_RS11855"
     CDS             complement(18204..18551)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172069.1"
                     /locus_tag="BK581_RS11855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4260 domain-containing protein"
                     /protein_id="WP_078579929.1"
                     /transl_table=11
                     /translation="MNKIILHIEGLVILSLSLYFYSYNQLSWVLFVLLLLVPDISMLGY
                     VINNKMGAVIYNICHTYILSIGVVICGLVLSNQTVLAIGIIWTAHIGMDRAVGLGLKYP
                     TDFKDTHLNRV"
     gene            complement(18763..19236)
                     /locus_tag="BK581_RS11860"
     CDS             complement(18763..19236)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /locus_tag="BK581_RS11860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="STM3941 family protein"
                     /protein_id="WP_143709675.1"
                     /transl_table=11
                     /translation="MKVRFIALGFLMCVVTFNDGDYVISALGAVTGLFFAFLFIMLVKQ
                     QLTSKPYLILTKEELITSAASKNPIPIAWQDIRGYNLIKVNGSKILEIMLEDEEKYRLQ
                     MTKTTRWLNKLNDAMNFAPFAIALGQVKRKDRDKLLGELDKRTFGEDLTMEEA"
     gene            complement(19630..20907)
                     /locus_tag="BK581_RS11865"
     CDS             complement(19630..20907)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019156537.1"
                     /locus_tag="BK581_RS11865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_078578379.1"
                     /transl_table=11
                     /translation="MSNKLTHSAHIYVDQPEKQKQLYKRTLIIVVISQIFGGAGLAAGI
                     TVGALLAEQMLGTNAFAGLPAALFTLGSAGAALTVGRLSNRFGRRMGLATGFITGGLGA
                     VGVVLAATLNSVFLLLASLFIYGAGTATNLQARYAGTDLATNKQRGTAVSIAMVSTTFG
                     AVAGPNLVDVMGDVALSIGVPSLAGPFILSAAAFLIAGLIFFIMLRPDPLIIARAIERD
                     KEEKNVNGISAHTGQMENKRGIVVGATMMVLTQIVMVAIMTMTPIHMSHHGHALSAVGL
                     VIGFHIGAMYLPSLVTGVLVDKLGRTTMAIASGATLLLAGIVGAIAPGDSMILLIVALS
                     LLGLGWNFGLISGTALIIDSTAPSIRAKTQGTVDVFIALAGASGGAMSGMVVAGSSFEM
                     LSLAGGILSLALIPVVVWSRKDKGSE"
     gene            complement(21059..21421)
                     /locus_tag="BK581_RS11870"
     CDS             complement(21059..21421)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897022.1"
                     /locus_tag="BK581_RS11870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_245829018.1"
                     /transl_table=11
                     /translation="MEKDVLQFKGGHNIALKIPKFKYEETVQFYKDVIKLPYLGFNSES
                     HAFQFGDVTLWLDCMDNYAQQDVWLEIQTNDLKEADVYLKQNNINRRDEVEVHEDSKGY
                     WISDPSGTIVRVNPER"
     misc_feature    21791..21804
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.7"
                     /tool="antismash"
ORIGIN
        1 attcgcccat actgagcgag ctaaagaaac cattcgcacg agtgaaccga agtttgtaag
       61 gaagcatatt gatcaaatgt taaacgacgt ttgggaagct taccgcatta tcacgattag
      121 ttcaagcatc cccattcact cactggatga catgtcagct taccttcaac aggcaaagaa
      181 aggattgtac ttacaaccta gtcaattaca acgcgtcctc tccttccttg agcattgccg
      241 caaacttaac acgtttatgc gcgataaagt gcctgtagcg ccaaacgttg ccaattatgt
      301 gttatcgata aacgatatga gtgatactga aaacgccatc gctggcagcc ttcgtcacgg
      361 tcaaattgat gatcatgctt caccagaact ggcaaatatc cgtcgccacc gtcggataaa
      421 gcaagcagaa ataaaagaga aagcagaagc aatatgccgt tcaaaaaaat gggctccgta
      481 tcttcaagac agcctcgttg ttgagaagaa cgacaggtta accattccgg taaaaaaaca
      541 atatcgttcg aaagtatccg gcactatgat tgacacgtct tcatctggcg ctactatctt
      601 tgtggaacca aaagaattag ccactctcta tgagcaatta aatgacttaa ctatggcgga
      661 aacgtatgaa gtggaaacga ttctttatac gttaactgcc agcatattag acatagaaag
      721 cgacttgcat atggccatgg acattatgca tcactatgac gtcctcttcg ccaaagcgaa
      781 atatggtcaa tccatcaacg ccgttattcc gaagcttaat gaagactaca tgatccacct
      841 tgatgcagca cgccatccac tactaggaga gcaagctgtc ccattgtccc ttaaactagg
      901 catacgagac cgtgcactgg tgattaccgg tccaaataca gggggaaaaa cggtgacgtt
      961 aaagactatc ggactgctta cgcttatggc acaaacgggg ctgatggttc cggctggccc
     1021 caacacatcg cttcacgtct ttcaaaaact atttgtcgac attggtgacg gtcaaagcat
     1081 tgaacaaaat ttaagtacct ttagttctcg cctaacgaat attattgaca ttttggaaat
     1141 aactgatgat aaaacattag tccttattga tgaactaggc tcaggcaccg atccgaacga
     1201 aggcatggct ctcgcccata ttattcttga acaattatat gctaaagggg cgacgattgt
     1261 agccacgact cactatcgcg agttaaaatc attggcacag acacacgatg gatttctaaa
     1321 tggctcaatg acctttgatg tggacacatt gcagcccact tatcggcttt tattagggga
     1381 gacagggaat agtcaggcat tcgatatcgc cttcaaacta ggtcttcacc caacactcat
     1441 tcaccgggct caacaattat gcggaaatct ggtcgatgag acatcctatt ccatagcaga
     1501 catcgaccaa acgcaaaaaa ggcgttatga caaacaactg gccacaacga actatgtgag
     1561 gacacaacag aaaaagaaaa aaacagctat ccctttattt aatcagggag acaatgtcac
     1621 agtatccccc catcaagaag taggcattgt ttataaaggt cctgatgaga aaggagacta
     1681 catcgttcaa attaaagggg aaaaacaccg cattaaccat aagcggctga cccttcacat
     1741 tccagcaagt gagctatacc caccagacta tgacttcgat attattttta aatcggtgga
     1801 ttatcgcaaa acaaaacacc tgcttgaacg aaaacacgta gatggcctcg tcttagacga
     1861 cgaagagtaa taaggggtaa aaatgttaga agggactgta tcgacgttat gatacagtcc
     1921 cttttaaatc atgcactcat aaagagtgcg acaaagcatc tatcaagacc gaactttaat
     1981 tacttatttc aatccacgca ctcataaaga gtgcgacagc gaaaatctcc caattttcag
     2041 gaattcactt accgtatttc gagatttctt taaattatgc cttaaatgag agagttattc
     2101 cctatcattc aggtttttct tggtgcgggg cctctaggga ttttatgtgc gcttatggtt
     2161 cgcaccagtt ttaatcggtg aacttcaatc aggacattag cgcctcgtta tctcccacct
     2221 aattagtata aaaagaagtg ctgccgctag caattcaaaa aagagaagtc atatacccaa
     2281 tagaaatcca gaagtattta aagctaagtg ggaggacgat gaaaaaagtg ctttctcaac
     2341 cagttgataa aaagctaatt tccttctgtc atcgtgagga tgtcccctta tcggtagggg
     2401 atcagattaa gaagccaata tgagaaatag gcggaaaaaa tccccttaaa tagtcatttg
     2461 ccataacaat agcttgaata ggcggagaga ttccgctaat gactcggaaa atctgaaaaa
     2521 tggatcgttt tattttgcat aaacggaaaa cttcctccta tcttcccaaa aatgagctct
     2581 cccctgcagc taaccgaaaa atctccgctt attttactct cataactcaa tcaattattg
     2641 ataagccgtc ttatctaaag gtaaagacat ttataccgag gttttttatt aatgacaagc
     2701 ctcaggtggt aacccgtcta aatctgcctt taaccgggta ttcccttctt tatctctaaa
     2761 caattaggat tattaaactg aaaattatga cattattgat acaatgctag tgaagttaat
     2821 tatataaaaa taattcgaaa tgtaaaccat cattccccat tcgtgaccat aactttaaaa
     2881 ttgtctctat tttgagccgg gcgttttacg aacgggttag ctgtgataaa tgtcgaaaca
     2941 ccctaagcac gaaaaactcc cccaccgtga cagagcttat acctgccacc tatatattgg
     3001 ggagcttaac aaaggcacca ttattcactc ctggacgacg attttttttc gatcgtaaac
     3061 gactacacct agtatggcga cacttaaaat ggcgcatacc atatacatcc tcgagtagga
     3121 aagcatatcc gctaccggtc ccataatgat accgcctaac gagacaccta gatccgccat
     3181 ggcaatgaac agccctaata acacattgcg actcgcctca ggtagaacaa agcttaaata
     3241 agtggttaac gtcgggtaca gcagtgcttg cgcggtccct atcaaaaccg cccctatgta
     3301 aaaaaagaag gcgccgccgt aaatggctat gctaacactc tgtgacccta acgctaataa
     3361 aagcatcgtc ccgatcataa aaggggcatg ccacttgcca tcggaaggta ttcttttcct
     3421 taatgtaaag cgagcaaaca ctacggtagc agccataatc ataaggaaaa tcccagcgtt
     3481 ccccttgtcc acttgaacag catacagagg aataaaaata gtggtagctc caaatataag
     3541 ggaaccgact aacatgaagg cactacttcg acataagtct ggatttttaa ctaactgact
     3601 taatgactga actatgctac cgcttgaatg agcttggtca gtcataggtt tggttttagg
     3661 agtatctacg ttagcactaa aaccaaacaa accagtcaac atggcgatgg tgaccataac
     3721 gatggcgaag tatcccatcc cgccttgcca aatgcctatt gccagcagcg gcccaataat
     3781 tcctggtata tatgaaaata acgaatacag ggaaatccct tgagaccggt cttcttccgg
     3841 taaagcatcg ataatcccta tttgtaatgc cattgaaaaa aacgctgtgg aaaccccttg
     3901 catcatgcgc gcaatcagat agccacccaa tcctgttatc gtatataaga ttaaagccaa
     3961 accattgaaa acgagtataa aacgtaatac ttttagcggc ccatgctttt gaataatttg
     4021 tcctgcccac ggtctaaaaa acatcgtcgt aaacaagtag gctcccatta taatgccaat
     4081 ggttgtattc gtcgctccta gtgcttcccc acgtaaagga ataaacacat ttaaaatagc
     4141 attggcacta aaataaaaga gtaccaatag atacaaacgc agaaaaggcc atgacatggc
     4201 tccattcaca cttttcccct ccaagctgta actattctct ctagcattcg ttcagacatt
     4261 tgctagcgaa cacctctcgt ttattcaata aaaagcttgt ctgacatatc gaacgaacct
     4321 tcaataattg gaggttttcg ctcttcccca ctgattgata attgcgtcaa tcaggacatt
     4381 accgcccgtt atctctcctc tctattttga accgaaagct gtacggacgg ttatctgtga
     4441 tagatcatcc tctctagcgg ttgacgttaa aacgtaatta acgcctttaa gagccgcttg
     4501 gcatatggtg aggtgacata cttcaattgg tcaattgcga tgatttcctc cacttgaccc
     4561 tctttgaaaa tcaatactct atcacacata taagcagcgg cttgtatgtc atgagtaata
     4621 aaaatatagc tcatcttata cgtctttttt aaccctttta agagctccaa aatctgtagt
     4681 tgaatagaca tatctaacga gctgatcgct tcgtctaata tcagccattc aggctctgtt
     4741 gcaatcgctc ttgcaatgca tacccgttga acctccccgc cggataattc atgagggtat
     4801 gtcgttttgt aggaaagact taaccctacg tcatgtaata attgatccac tttctgacga
     4861 gtgtttattt tgttcttact catcatgagt ggttccataa tggcgtcctc aactgtaaaa
     4921 aaggggttga tagacgattg atagttttga aacacggcac tcatgcagcc taatctgacc
     4981 tgtctgttgg taacaatttg tccgtttaac gtgatgtttc cttgatccgg tttttcaacc
     5041 cctaacagca tacgccctaa tgtggatttg ccactgccac tctcaccgat aatgcctaga
     5101 cattcgccag cctgacagtc aaaacacaca ttatgcagta ctttctgctt tttggaagaa
     5161 aacatcccgc cttttttgta ggacttggac acatgactaa ccttcagcaa cgatagcgtc
     5221 tccttccatt attttcttaa aatggtcact taacgctaat ctagtcgaca ctaaatagcg
     5281 ggtatacgca tgctctgggt cagaaaaaac agcttgtgtc tgacctcttt caacaatctc
     5341 accattcctc atcactaaca cgttgtccgc tagctttttc acaatagcta aatcatgaga
     5401 aataactatc atagaacagc ccatcttttc ccgcagcgcc atgagctgtt ctaccacttc
     5461 aaactgtgaa atcgtatcaa gcgcggtcgt tggttcatca gcaaaaatga tatcaggctc
     5521 tatcacaata gctaacgcaa tcatcattcg ttggagcata ccaccagaca gttgatgcgg
     5581 atattgcgtc ataatatgag ctgggttttt cagcatgaca ctttccatcg ctttcatcat
     5641 tctttcttcc atctctttta tactccaacg aaaatgctga gtgagaatcc cttttacata
     5701 acgtccgatc acacaagagg gatcaaatgc gcccatgcca ttttgtaaga tcatatacag
     5761 gtgtttgcct ctcagtcttc tcattttctt ttccgcaagt gtattaacgt tgtgaccgtt
     5821 aaataaaatc tctcctgatt gccttaacgg aactttattc aacttcataa gcgctcggca
     5881 cgtcacagat ttaccgcttc cgctttctcc aacaatcgcc aggcagcttc cttgctttaa
     5941 atcgaaagaa ctattatgaa taatcgcttg tcctgtgtgg ctattccaga cttttagatt
     6001 gttaacgttt aaaagtgttg tcatcatcag atcacctctt tttccttgat cacaactttt
     6061 gagagaggtt tgccgccgac tttacgttgc ttagcgttga ttaatttggg atctaacgcc
     6121 acttgaagcg catcagataa aaaattaaag gctgacacga caatgataat ggctaagcca
     6181 ggtgctaaca tgacttgtgg ttgcgtaaac ataatgtctc tcgcctcgtt tagcatcatc
     6241 ccccactcag cattaggcgc ttgaatgcct aagcctaaaa acgagaaacc ggatatttgc
     6301 aaaatcattg acccgatagc actgcttgat atgaccgcaa tatctaaaaa cgtaacgggt
     6361 aacaagtgtt tgtacataat ggtcacatga cgcatgccga ttgccttgga aaaagtcaca
     6421 tactccaatt cagcgtattg catgacagac gttctaatga cacgagcaaa ccagacccat
     6481 ttcactaaaa taaacgcgat taatatattt tcaagaccga cgccaaaaat accgacgaag
     6541 gctaatgtca tgacgtaacc ggggaaagag agcatcacat cacagcacct cataataaca
     6601 gcatccactt tccctctaaa ataaccggct aaaaacccta aaagggcacc gattagtaca
     6661 gacacaatta acgctacgag cacggccaat acactgggac gaataccata tatcagtctg
     6721 gacaacacgc atctccctaa gtgatcatta cctaaaaggt atgaccatga cggtgaggca
     6781 tgtcttaaag ccatgttcac gtcgttaggg ggatgaggcg cacataaagg agcaaaaatg
     6841 ccgacaacaa ccgtgatgac aatgattatg agagaaatag cggctaactt gtcttgacct
     6901 agttgtttta acaacttcat ttaaagatca ttccttatct tcggattcat cgccgccgtt
     6961 ataatgtcag aaagcgtatt aaatgcgatg aatgccactg ctaatatcaa aacatacgct
     7021 tgaataatcg ggaaatctcg gcttaagatc gctctcaccg ttaaacttcc tagccctggc
     7081 caggcaaaaa cattttctat caccacggta cttcccaata taatcggcac tgccatccca
     7141 aaaatagaca tagccacttg taatgaattt cttaaaatgt gtatcgtgat tgttttttct
     7201 ggtaacccgc acgctctccc atataacaca taatcttcat tcaagttact tatcatcgac
     7261 gttctcacat ttctaaaata aatacctgca taacttatga cgatgaccgt cacagggagg
     7321 atgtagcttt gataagacgt catgccattt gtcggtaata aatccaactt aacagacaca
     7381 aaccatatca tcatggatgc aagccaataa gacgggatcg ctgtaaggaa aaaggataca
     7441 cccctgatcg acttatccat cacccttcct tcctgcaatg cgcagatcac ccctaataaa
     7501 atcgacaaaa tgatgatgaa aaaagaggac acgattgtta atttaaatgt attgaggaaa
     7561 gccggcccta ataaggacca aaccggtttt cccgtcacat aggacgatcc aaagtccagc
     7621 tgtaaagacg ctataagcca attaaaatat tggactatga aaagtctatc catgcctaat
     7681 tcttctttcg tctgcgccat taactcatct gtaatcgtcg gcactccttg cgctttcaaa
     7741 accacttctg ctggatctaa tggcgcaaga ttaatgaata aaaacgttaa gaacgagatg
     7801 attaacagca tggggatggc gataacgact ctttttacaa tataccttcc cataaaaatc
     7861 tccttccaaa tttagcagac aatctcactt tctttataaa acgctccttt catcaatggc
     7921 tgctttcgcc cttcctccag tcaaagagga cattatcggc cgttagctca cgcctaaata
     7981 gacctcgctt ttctctctat tttgaggcgg gagctttacg gacgttatct gtgggagaaa
     8041 ttctcctatt taaaggacat gtgctcaaat ggcagctcga attgtgtttg cttaaaagaa
     8101 acaccttcta gctctgctgg cgccactacc gtgagactgc catttgaaat aggaataaac
     8161 acagcttcgt catggacgat cgtcaaaata tccccgtaga gattttgtct catgtcttca
     8221 tccgtcgtca ccattacctc ttctattttt tgataaagtg tgtcggcttc cgcaatccca
     8281 cttgtggtat gaagatatga ggcttctgat gtaaacgcag cgatcgtgct ttgcggatca
     8341 tacgccaacc cccatgtttg attaaataac aactcatact ctcctgttga ccgtctatta
     8401 gcgattgaag tggattcttc tccgacaatc tctagttgaa caccagcttc tattaaagtg
     8461 gcctgaataa attcagcctg tgttttttgt gaagaagagt gactatcata ataaagcgtc
     8521 atcgttaatg tgttcccgtc cttcattcgc acgttagacc catcctctaa taaccaacct
     8581 gcttcatcta aaatgtccgt cgctttttct aaatcaaatg atcttacgtc caaatccaca
     8641 tcagcataca tgatattaga agaaaagagg gtgtccgcca cggtttcact accatctaaa
     8701 atctcctccg caatcgtttc ccgatcaatg gcatgccaaa tcgcttctcg tacagctcgt
     8761 tcactcactg ggctctcatc ttgactacta ttggccacga tcattttcgt gttcatggca
     8821 tcacttctaa tgagttggta cgcccccgaa gctactaatt gatcaatcgc ttccacatcc
     8881 agcgtgtcaa caccgcgatc atccgtaaaa acaaagttta cttccccttt ttgtaaagct
     8941 aaaaacgtcg tttctcctgc tgggagcact ttagacgtaa tcgtctctat ggcaggctcc
     9001 cctccccaat aagcgtcatg ggcggtgaag gtggcgtact catcgacttt atgatctgtt
     9061 aacatgtatg gtccggtccc atgaaaccca cttacaccct cttttgtttc cccatctata
     9121 aaatcgtttg gagagataaa aacgtaaggt cgcgtcatgg ataactcaac taacgttgga
     9181 taatacgctt cagataatgt cagctcaacc gtgtactcat caacgacacg gcaatcttct
     9241 atttgtgtag ataatttaat ccatgcatgc gtctccgcat tagcttgtac ggcctcaata
     9301 tttttcttca ccgcctcggc attaaacggt tccccatcat ggaaggtcac atcttccctt
     9361 aaatgaaatg tgtagacggt gccgtcatca gatatctccc aagactccgc taatagcggc
     9421 ttaataccct cctctgtatt ttcaacgagt gattcatata tcatcccttg ggctggcatt
     9481 gaccctgtat acagatgagg attcatatca ttgatatctt gtgccgtagc atagacgatt
     9541 tcctcttgag cctgggtgct tgcctctatg tcagcatccg actcctctcc ccctgcgcaa
     9601 gctattaaca cccttccgat taaaactata aaacttacaa acatcatctc tttcactctc
     9661 aatgtacgtc gctcctttac cttgcgttaa gtatgaacat tcttgcattc ctatcattga
     9721 acatgttggg taaaatcatg gctccctttt ccattatgaa aaggccactt ttactatgtg
     9781 aggtctggct ggccttcgta ttgggcgttc cgctcgtttt aagaaaaacg aaacgtttac
     9841 tgttttcata aaaaacttca attagtcgcc gttttcgtcc ttctcctact gttaaaggag
     9901 cgtcaatcag gacatagcgc ccgtcttaat agatgtcgct ctcctctcta ttttgaaccg
     9961 gcagctagta tattttaaag ggacaggtca tctctctttt ttaggaagtg acggatttta
    10021 aatttcggac gatcatcgcc acatcatcat gaaacgtttg aacacggaaa gcatcagata
    10081 aaggctttcc atcgtgagat tgtgccgcat tacgaatggc ctcttcatat gtttgtatga
    10141 agtgatcgat tgtgtcgctt tttacttgta aatgatgggc aattccttgg atgatcttta
    10201 aacggtaata gtcttcctta ggcattcttg gtatatctaa atacccttct ctgttaacga
    10261 aaacgggtct tataggaacc gcagaaaaat caaaatagcg gccatcctta tccggttcgg
    10321 aaaaagggtc aatcagcaaa gatgtataac ggatatacac taaatactct tgatggattg
    10381 gagctaactg attaaaattt tctatatctt cccgtggtaa gctttccggt cttactgggt
    10441 aattgtcctc tgtcataaac ttcagcaagt tgatgccgtc aatgttaagc gcctctaaaa
    10501 ttcccatgat ttctttccat tgggccaaca tatggcgaat taaagcgtac gtaatcgggc
    10561 cttctggaaa cattttgtat acgtatgttt tcgtttcttg ctcttcaaaa atgtgacgaa
    10621 gtgagaaatc attcataaat aatggcggat gaacgtataa ggagatattt cgcgattctg
    10681 cctcaatagg tgatctcatc acgtttacat tcacccctaa tgtgtcataa aggtcacata
    10741 atgtgttaac attttccgat ttaccatgcg tagaaccgat agaaaggtta tgtttgaccg
    10801 ctgtcgttaa aacacgatta gacggcgtat catctcgcca tcttgtatcc cccaagtacg
    10861 ttgaaaagct gataacctct atagtgtcat caaaagcact catataagcg tccactaaac
    10921 gattggagcc gaacgtcggg gaaattaaga cgacacattt gactccttct aaaaccgttt
    10981 tatccaactg cttgagtaca tctagataag catctgttgt gactgtcaga atgagcgtat
    11041 cccaatcacc tttaaccgtt tcatagcttg taaacacctc gtcaaccgcg caatcacctg
    11101 ccaggttgtc attcttgctg ttttgagtgg tcacgtctag acgttgatgc tggtctgtta
    11161 aagcgtcaaa aaaagcgtcg gagcgaacag attttcttcc ggcgatacca atttcacacg
    11221 tgagatgacg tttaataatc accgcaagtt gaaccgcaac aggtcccgtc cctaatagta
    11281 agactcgctt aaaagcattc atctgatcct tcctttctca gcatgtctaa gctatgcctt
    11341 ttgatacaaa ataatgtcaa atacatgctc tggacgtacg attgtgttga ctttccgcca
    11401 tttatgctta tctacctctc ggcttgggta attaaataac gatttcaact ggtcaccata
    11461 acgcatggcc accactacgt cattacgggt taatggatgt aagtgatcta gtagatcgta
    11521 cttttgttca acagtggaac taaaaataac atgagagacc gtttttagct tatctatgtt
    11581 ttctattggt gactgttcta acctcacatt taatcctttt cctagttttt caacgacttc
    11641 tctgccaagt tgtaccgcct cgtcatcaat atcgatccca atcacatctg ctcctgtccg
    11701 cttagcaatt agcaatagtg tcatggggaa tgcacctgaa ccgactaata agacctttga
    11761 cttcgctgtg acatgaaagc taccaaactc actttctata cttgactcaa tgtttttaaa
    11821 atagtctgtc atgctttcat ggtgatttaa tagttttaat gcgcggtatt tctccataat
    11881 tgccacacag cgcgctgatt gtcttcttaa gttcaccaca agttgtgcta actgagcatt
    11941 gtcttcatgt atgagctgtt cccatacgtt tttattcgcc tcatgtgtaa tgaatgtgga
    12001 atactcatcg attaacctct ctaactctgg actgtaagtc atcgtatcat catagttgcc
    12061 tgctaaatca tcaaatttag ttaaaagcct actcaaatac ccttgaaagg ttgttaacgt
    12121 attcatttct tcaccccaat atgcttagtt aaaggtttat gtaagctgtt cctttggcaa
    12181 cgagctcaac acttccttca atgttaagat cgcgtatttc tccagctata taatcccctg
    12241 acaccgtgat agagccaccg ggctggctaa tatccgctac agtcttcctt ttatttttcc
    12301 aagtgagata ggctccgata gaagctgtcc cagatccaca acctctttcc caaatcatgc
    12361 tatcgaggtg tggcacatag ataagtggtg ccaattcatt ggatataggg ctatacatta
    12421 aaataccgat tagatgggtg tttagagtcc ctcctaacag ccttgctaac gactctgctt
    12481 tgtcctttag tgagtcattg aaggtgtcag cctctatgat gagatgaatc gcttctctat
    12541 aggtgacgaa cgtgacagac agctcacttc cttcatagtg taaggtcgtt tgctctaacg
    12601 ttgtaggaat aggcatgttc agttgacagt aatacttatg gggcgttctc ctcacttggc
    12661 acgtgattaa gtgatctgtg cccgtaactt ctaacacgat atctaacgtt tcattaagtt
    12721 ccagcccttg ttcgaccgcc tttaaagtgc ctaatgccat gcatgcatta ccacaaaact
    12781 cacctcctgc catgcgaagt aatgcggacg cttttttgtt taaaggtgtt ttaataaacc
    12841 ccacttgttc cccatacaca ttctcgtagg ccattatttt agtggcgatt ttttcatagt
    12901 cttgcaccac attgctatca tcaattagaa ttgtcatgtt ttgagtcggg ttaaatttca
    12961 caaaatctat ctctcgcttc atcttcacca ccctctaaac gccattcata tgacttatta
    13021 ataggttatt cgttgataat cgtaattatt acgatttgct ttataaaagg attatagcat
    13081 aatattggca gtcgtcaact tttaatttaa aaaagtaaat ccatttaata atagaaagaa
    13141 cacctgtcaa aagtgactca tccagtgatc ttctgataga gatgggcgac taaatggggc
    13201 gataaaatct tttcttataa ggggaatttt cagacgtcac tgaggcacta ggaaccaccg
    13261 tcatttactc catcccagac gattgatcat tgccgcttgt tagtgttgaa cattcgatta
    13321 tgtcagttat tcacggtata taactgaaaa agaaggaatg cctcagtggc atcccttctt
    13381 ttaaagagcg tatttaatca tttaagtacg gtgctcttat ggttagttta agtcttccag
    13441 agtctactca ataatttctc tttcgaaact aaagggaccc tcgctttcag ccaaaaatgg
    13501 gtgtcattgc aatatcggct ccacattcgc tgacctgtct tagttcataa agaaaatacc
    13561 gataataaga gccatactca ctatccccca aaacgttgtt cctactatcc acaagatgat
    13621 aggtgtatta gcatttttat gattatcagc gttttcttgt ataaaggcga gctttctttc
    13681 catttttccc ttcatagaaa tgacaacaac aaatccgata atgatgaaac taaccgttag
    13741 gaataaaacc atttttatcc ccctttttgt gacctgctct ttgcatctcc ccctagttta
    13801 acatgtatgc acgtatgaat tcccgtcaat agaacaatta tttaaactct ttttctgtaa
    13861 atgctcttga tttttttcac acattttata aacgccctag tcaacacagt cataaatgag
    13921 cacaacttga aaactattaa aaaatgattt tgtataactg ataacctatg aagagagaca
    13981 ctccccaaat catgatcgca gatactttgt taatcattct tacaatcttt ccttctttat
    14041 caatagatcg tatggtttta ccaatgtatg caagggcaaa aaaggagacc caagatacga
    14101 gaatacaggc cgctgcgaat gctattttct ctggaattgc ggtatacctt atagagctag
    14161 ttccgatgac tcccactgta tctaaaacag catgagggtt taaaatcgag acggaaatag
    14221 caaacatggc ttgcttttta cctgtcatgg cggcatgttt tttgttcact tcagatgtat
    14281 tgctattcca gatcgaccag cctatataaa ttaaaaagaa aaaaccaata gcaaaaaaga
    14341 ttaactggaa ggccggtatt gtcataacga tgaccgacac acctagaact gccaatatta
    14401 ttaataacgt atcacacaga gacgctgtga tcactgcagg caaagcaaat ttgactttac
    14461 tgtgggcagc gccttgatta aatagaaaga tattttgagg gcctaaaggc aatattaagc
    14521 caaaagataa tatcatgccg tgtaaaattg ctcctaacat ttaaaatccc ctaactcatc
    14581 tattttattt ttgccgttga taacggctga tttttttggt tgcatcactg caaatatctt
    14641 ctatgacact tttaaatcct ggtaggaatg ttttaaaaaa gcctgtgcaa tgatgggctt
    14701 ttttataatc atagtcttcc attaaaatta aatggacttt ctttaaattg tagtgtggaa
    14761 ctgccggaaa taaatgatga actaaatgat aattatcatg gtgtgggtgc aaaagaaaga
    14821 tttgaatagg attgccaaaa gtattacggc tggcatataa ctcgtggtca ttttttagac
    14881 cagaatgttc cgccatttct gcccaatatc taatgacctg aaaagtcgtt aataaaggaa
    14941 cgaaccagaa caaaataata tgtaaccaaa agttaaaaat gatactgcat gttattataa
    15001 tacaccaaaa tatgggcttt gcaacttttt cagaaagcgg ctcttcttta gacataacat
    15061 tcacttttaa agtcccgtaa atatattttg gcacgtgagt taatgttaat ggcttaatga
    15121 tgtgtttaaa aataaaagtc ttaatacttt tttgtggttt atccaatcca ataagcgaat
    15181 acctttgagt gtctgggtcc ttgtcaatat tccagagatg tctatggtga gcatggtgag
    15241 agttgcaata ggctgtaaac gaagtgaaaa caggaaaggc aacaaaaaaa cacgttatcc
    15301 atttgttcat tattttattt ttgaacagaa gaacgtggct ggcttcgtgc attaaattat
    15361 caaaagctcg cattctactt ccgataatcg taatggctat caaataggac caccagtttg
    15421 ataataatat ggcgccataa gccgacgcta ttatgactag ccaatcaaat aagatggaaa
    15481 atagattcgt ataattatta cgtgtttgaa gcgctcttac ttgatttaca atctctttag
    15541 agaatgttgc tttctttaat tttttgtcct cactcgtcat tacagtcatt ggtcaacccc
    15601 tccatcatca tagattctac ccaatcttta aatggttaat gtctacatgt tcatctattt
    15661 cacagcaatg accgcgccta tacttatctt cttctctaat gttatctgcc cttttttctc
    15721 cttttaaagt tctattagga gcataagtgt tatattaatc aaatgtcact attttaataa
    15781 ctagacatgt tccaagttat tcatgattcg gtttttacac gccttttctc tactaagtag
    15841 caggattctc tcttgttgcg acactcaatg cccttcttac ggctgagtgg cgctggttct
    15901 ctcttttcgc gacactcgat gctcttcttt cgactgagtg gcatggcttc tctcttttcg
    15961 cgacactcag tgtgcttctt acggctgagt ggcgcagatt ttcccttttc gcgacactca
    16021 gtgttctttt tacggctgag tggcgctggt tttccccttt cgcgacactc aatgttcttt
    16081 ttacggctga gtgacgtggt ttctctcttt ccgtgacact cgatgctctt ctttcggctg
    16141 agtggcgctg gttttccctt ttaacgacac tcgatgccct tctttcgact gagtggcgct
    16201 ggtttctctc tttccgtgac actcgatgct cttctttcga ctgagtggcg caggttctcc
    16261 cttttcgcga cactcaatgc tcttcttacg gctgagtgac gcaggttttc ccttttcgcg
    16321 acactcgatg ctcttttttc aactgagtgg cgctggtttt cccttttcgc gacactcaat
    16381 gttctttttc tggctgagtg gcgctggttt tcccctttcg cgacactcaa tgctcttctt
    16441 tcgaccgagt ggcgtggatt ctcttttttc acgacactcg atgctcttct ttcgactgag
    16501 tggcgcaggc tttccctttt cgcgacactc agtgtgcttc ttacggctga gtggcgcaga
    16561 ttttcccttt tcgcgacact cagtgttctt tttctggcta agtggcgcca attcacacaa
    16621 gtgacgatga ctataaaaga ttatatccta caaaattatc ctaaatcaac cataatttat
    16681 taattattag taaatttatt ctaaaggtgt gtaataaagg agcaagctgg tgaagagagt
    16741 atgtcgtgtc aacagtgggg gacttcacta agtgaaataa tcaataaacc atagtaaaat
    16801 ttaactgaca gcacatttag aaaggacggg atgtagagtg ggtataaatt ttcatagtga
    16861 taagaatagc acgacttata caactcgcaa ggctgacaaa acatgggtgg aagctataag
    16921 aaaactcgtt cctattgaag aagtgtctta tgctgcagat attggctgtg gtggtggtat
    16981 ttattctaag gcgttatcgg atatgggagt ggatgaagtc actgccctcg atttttctaa
    17041 gtccatcctt aacggagcga aagaaaactg taaggactat caaactattt cttttaaaca
    17101 tggcaatgct tttgaaacag gtttagctag tcaccattat gagttaatcc ttgagagggc
    17161 tttaattcac catatagagg atttaaacgc ttgttttaat gaggcattta ggttgttaaa
    17221 agatggtggt tactatattt tacaagatcg tacgccagag gattgtttag taaagggtga
    17281 ggacactcat attagagggt atttttttga actctttcca aagttagttg aagtagaaac
    17341 gagccgcaga catcatagtc aacatgtcat tgacatgcta aagaaagttg gatttaagga
    17401 tgttgaggaa gttaagttgt gggaaacacg acatgtttat catactaaag agccgttact
    17461 aaatgatttg tatgaaagga ctggacgaag tattttacat gaattagaag atgacgagtt
    17521 acatttagta atcgatcata tagatcaatc attacctaaa aatacaccta tcgttgagaa
    17581 agataaatgg accatttgga aggcagtcaa gtcggtttag tagtttgagc tattctttta
    17641 aacaaagggt gtcatatcag tattgtcata aaaagcgaat aagaaatatt cgcttttctt
    17701 attcgattat atattctact ccgttttcat ccgtgtaaga ttctaaaatt atgtcgtcat
    17761 ttttatcttt ataataaaag tacgtattgg tatcgttttc tatttttatg cttaccatat
    17821 agtttcttaa tcctggtaaa ttagctataa aagcttcact agattctata ctctctgttg
    17881 gaatcttcat ttcaaccatg acgtgttcaa ttacatcact ctctagttta ttctttgcgt
    17941 agaaaatata ccctgaaata ccgatcccaa tcattaatat aatcgatatt ataatcctaa
    18001 attttctttt cattttcatc atccttttcg aaaatggcac aatagaggta atatcatgaa
    18061 aagattttta atattgtttt caactttccc tttaaaaatc gtcatggtgt gcgtattaaa
    18121 tatgtgtttt ttaaggaaca cgtctactct attaaaatca ttatctattc caattgctaa
    18181 ggcgccgtta aatgctaatt aggttaaaca cgatttaaat gtgtgtcttt aaaatcagtt
    18241 gggtatttca atcccaatcc aaccgccctg tccataccaa tatgagcagt ccatattatc
    18301 cctatcgcta agacagtttg atttgataac actaagccac atatgacaac gccaattgat
    18361 aaaatgtatg tatgacatat gttataaatc acagcaccca tcttgttatt aatcacatac
    18421 cctagcatcg atatatctgg aactaataac aatagcacaa ataacaccca gctcaactga
    18481 ttatacgagt aaaaataaag actgagtgat aagatcacaa gcccttctat gtgcaaaatt
    18541 attttattca cacccattcc ccattccttc tttattcatg agtaaaaagc ggtctctagg
    18601 aaacgatggc tcttttttaa tgttgtcatg tcgattaatc attttagaaa gcgactgtac
    18661 atcgatcttc cactacagca tgatgctttc tcaagggctc atcttcagct tattaatcac
    18721 caacgttatc attcttaaca tggttagacg tccgccattt catcacgctt cttccatggt
    18781 tagatcttca ccaaacgtac gcttatctaa ttcgcccaac agcttatctc tatccttgcg
    18841 tttaacctgc cctaacgcaa tggcaaacgg agcgaaattc atagcatcat ttagtttgtt
    18901 caaccatctt gttgttttcg tcatctgaag acggtatttt tcttcatcct ctaacataat
    18961 ttctagtatt ttagagccgt tgactttgat caagttatat cctcttatat cctgccatgc
    19021 aataggaatt ggattctttg atgcagcact tgtgattaat tcttccttcg ttaaaatcag
    19081 ataaggtttt gaagtcagtt gttgtttaac taacattatg aataagaagg caaaaaataa
    19141 gcctgtaaca gcccctaaag cacttatgac ataatcgcca tcattaaagg taacgacaca
    19201 cattaaaaac cccaatgcta taaaacgaac cttcaatcag tgggagtttt cgttcttctc
    19261 ccactgattg gtagttgagt gaatcaggac attagcgtcc gttatctccc gcctaaatag
    19321 atttatctct cctctctatt ttcaggcggg agttttacgg acggttatct gtgataaatg
    19381 ccaaacacaa tagcagtagt atgaaatttt tacccttcga tggataaaat tgtagttctt
    19441 ccataatccc cttcaacctc caaattctct ataagtgaca ctatattata accagtgcac
    19501 actatttttg acaacgggat atctgcaatt aaccaacgat gggcatgttg tttagttacg
    19561 ctacgccata gattagcaga tgagagattg ggccgctaat gtgtttatac ccatcacact
    19621 cacactctct cattccgaac ctttgtcttt acgagaccac acaaccacgg gtataagtgc
    19681 taaggacaaa atccctccag ccagtgatag catttcaaaa ctggaaccag ccacaaccat
    19741 tcctgacatg gcaccaccag aagcaccagc taatgctata aaaacgtcga ctgtcccttg
    19801 ggttttagcc cgtattgaag gggctgttga atcgataatc agtgctgttc cgcttatcaa
    19861 accaaaattc caccctaaac caagtaaaga aagggccact attaagagaa tcatagaatc
    19921 tcctggcgca atagctccca caatcccagc tagcagcaac gtagcaccag aagcaatggc
    19981 catcgtcgtt cgaccaagtt tatcaactag aacgccagta acgagagaag gaaggtacat
    20041 cgcccctata tgaaagccaa ttacaagtcc tactgcactc aatgcatggc cgtggtgact
    20101 catatgtatt ggagtcattg tcataatagc taccatgaca atttgagtaa ggaccatcat
    20161 agtagccccg accacaatcc cgcgtttatt ttccatttgc ccagtatgtg ctgagatccc
    20221 attaacattc ttttcttctt tatccctttc gattgcccta gctataatta aaggatctgg
    20281 tcgaagcatg ataaaaaata taaggccagc tataagaaat gccgcagctg ataagataaa
    20341 cgggcctgct agtgacggta caccaattga aagagcgaca tcccccatga catctactaa
    20401 gtttggacct gccactgcac cgaatgtagt agataccatg gctatactca ctgccgtgcc
    20461 tcgttgtttg ttagttgcta agtcggtacc agcatagcga gcttgtaaat tagtcgccgt
    20521 acctgcacca taaatgaata gggaggcgag taatagaaac acactattta aagtagcagc
    20581 cagtacaacc cctactgctc caagtccacc tgttataaat cctgttgcaa gccccatccg
    20641 tctaccaaaa cggttagaaa gtcttcctac tgttaacgcc gctcctgctg aacctaacgt
    20701 aaataacgca gctggaaggc cagcaaatgc gtttgttcca agcatttgtt ctgcaagtaa
    20761 tgcgccaact gttatgccag cagctaaccc tgcaccacca aatatttgtg aaatcacgac
    20821 aatgattaac gtacgcttat ataattgttt ctgtttctct ggctgatcga catatatgtg
    20881 tgctgaatga gtcaatttat tcgacatgtt gaatacctgc ttttccctta gtttacttat
    20941 cccttaaaag aaagatctcg tgtgaattta acaggcttta cggacggtta tctgtcataa
    21001 agaaatcatt acccttgctc attgttaaca cgtcatgctt tcctgttaaa acgcccttct
    21061 acctctctgg attaactcta acaattgttc cagaagggtc agaaatccag taaccttttg
    21121 aatcctcatg cacctctact tcatctcttc tattaatatt gttctgcttc aaatacacgt
    21181 cagcttcttt cagatcatta gtctgaattt ctaaccatac gtcctgttgc gcataattgt
    21241 ccatacaatc caaccataat gtaacatcac caaattgaaa agcatgtgac tccgaattaa
    21301 accctaaata tggaagctta atgacatcct tgtaaaactg aacggtttct tcatatttaa
    21361 atttaggaat cttcaaagca atgttatggc cgcctttaaa ttgtagcacg tccttttcca
    21421 aatggttaac ctccatttcg ttggcatatt cttttatagt atcatcatat gtttttcctt
    21481 agcatcccat ttcttaggga ttgttcattt tggtaagctg ttttttaaaa gtcggtctta
    21541 gaatgccaac tgacatgttg cttcacctaa cgctgtaaaa cacctgacct gcgccttctt
    21601 ttttcctgct aatcacttct attacgataa cacgtcggta tgggcgagaa gatgctggtg
    21661 tagacatgcg taagagcgtg cctgcactat atattatcat cctcgtattg agggaactaa
    21721 tagactcatt tttattgacg gattatcttc tcaattcatg aggtccacct aaagcagttt
    21781 aagttttttg ttttttcaaa aaaagagttg ccatgcctca ccgctttaga tatgtttatt
    21841 taagcaaagg tattttaatg aaaagaagac gtgattgacg tgagattatg atgaaaaaaa
    21901 ggaggtaaca tcatgaacaa acgattttgg cctttattga tgttttcact tattattctt
    21961 tcaggttgtc tcttgaaggg tcaagcagat cctgacttct caagagaaaa atatgatagc
    22021 tggtatgaaa aagaccaata tgaattggct ttagaagatc tgaacgaacg attagaagca
    22081 taccctgaag atccatacct gcataatgag aaaggctatg ctctcc
//
