LOCUS       NZ_KV917378            19005 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Salipaludibacillus agaradhaerens strain DSM 8721 Scaffold1, whole
            genome shotgun sequence.
ACCESSION   NZ_KV917378
VERSION     NZ_KV917378.1
KEYWORDS    WGS; RefSeq.
SOURCE      Salipaludibacillus agaradhaerens
  ORGANISM  Salipaludibacillus agaradhaerens
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae.
REFERENCE   1  (bases 1 to 4319439)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Genome sequencing project for genomic taxonomy and phylogenomics of
            Bacillus-like bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319439)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2017) Agricultural Biological Resources Research
            Institute, Fujian Academy of Agricultural Sciences, No. 247, Wusi
            Road, Fuzhou, Fujian 350003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 227.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002019735.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 15:23:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,010
            CDSs (total)                      :: 3,909
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 101
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 17 of 38
            Pseudo Genes (incomplete)         :: 21 of 38
            Pseudo Genes (internal stop)      :: 7 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:59:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1960717
            Orig. end                         :: 1979722
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV917378.1.
            Bacteria and source DNA available from DNA is available from: DSM
            or Fujian Academy of Agricultural Sciences.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..19005
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="4"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..19005
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..19005
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:14005](-)"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..14005)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     misc_feature    complement(289..302)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.96"
                     /tool="antismash"
     gene            416..952
                     /locus_tag="BK581_RS09250"
     CDS             416..952
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627269.1"
                     /locus_tag="BK581_RS09250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_078577901.1"
                     /transl_table=11
                     /translation="MLTERQTERLVMRQVKAEDAESLFPIWSDPRVTQFMNITTFSHQN
                     EAVDMINYLNELAEQQQALRFSIIEKKSGRIIGSCGYHLLDLKNEKTEIGYELAYPYWR
                     KGYGTEVVKDLIDAAFLKLNLHRIEAKVEPNNVNSIRLLEKVNFLYEGTLRESEKSGDT
                     YSDLQLYSLLKTDSR"
     gene            complement(1183..2529)
                     /locus_tag="BK581_RS09255"
     CDS             complement(1183..2529)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082618.1"
                     /locus_tag="BK581_RS09255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MATE family efflux transporter"
                     /protein_id="WP_078577902.1"
                     /transl_table=11
                     /translation="MRKPALDQTSITTKQYLFLVIPITLSAVTTPLLGAVDTAVVGRMP
                     DPTYIGGVAIGTLIFNTIYWLLGFLKVGTSGFTAQASGMKQPQELIAAFVRPALLAVSF
                     GLLFIILQIPIEKSAFLLIDTTPSVKQLASDYFSIRIWGAPFALLNYCVLGWLIGSARV
                     KLSLALQLYMNISNIFLSVFLVLGLNLGVKGVAFATLFAEVSTVIIGLFVLNKLAIFSF
                     ENWRTNAFLQKEKFVSMLKMNRDLFIRTLCLLSMLGFFTAKGSELGVEILAANAILIQI
                     HYLLAYMYDGLAGANSILTGRAFGQSNYELFKRTKALSFIWGLGLSVVMVILLFVFKEP
                     FLALFTTIDSVKDLALTYYFWILLYPLVTFWGLLLYGMFTGATEGAPIRNSMIVSLILF
                     FAVYALTFPSWQNHGLWLAFIVFSFGRTIFLWAYISRLHTKIAQVCKAA"
     gene            complement(2566..2814)
                     /locus_tag="BK581_RS19945"
     CDS             complement(2566..2814)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK581_RS19945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_143709657.1"
                     /transl_table=11
                     /translation="MTTQLQILDLLIELKKEYHLSYVFITHDLTAVTYICDRVMFMKNG
                     LVVESIDQMENLPHLTHRYSKKLLTSVLDIGCHHQRQ"
     misc_feature    complement(2937..2955)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            complement(3120..4349)
                     /locus_tag="BK581_RS09260"
     CDS             complement(3120..4349)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF017760.5"
                     /locus_tag="BK581_RS09260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_078577903.1"
                     /transl_table=11
                     /translation="MSLANLEEKTKLKEKQVWKNKNFLFLLLGHSAHNFTFHIFALSIP
                     LIIYDLTSSSFYMGTMKAIEFIPNVVLGILIGVLIDWFNRKRIMLLSILIKIFSVIFII
                     LLLATSTIQLWHLYLLGFFIYTCIYAFANTYQSIIPSIVDKSQLTTANAYITFTNTIFN
                     SIGPAMAGAIILYTTYVVGLSITLLGLIVLLVCVLPVKLPSYEPRSHFNLKKDIIEGWT
                     QLYGSKALFSATLLILGNNIASVSTSTILIFYALDVIGITEGQLGLIFSISAIGGVVAT
                     LIAKYSKDYVNRGYIFILATFTVSLAQFAIFLSSNWYVICMSMFFVGFSATICNVHYFA
                     LRQESTPSHLLGRVIGTSSMIMKLALPFSFLVITYLADIIEVNLIFLMTSMFLLIIAIL
                     ALRTRLTDLK"
     gene            complement(4388..4966)
                     /locus_tag="BK581_RS09265"
     CDS             complement(4388..4966)
                     /EC_number="2.7.7.65"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /locus_tag="BK581_RS09265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diguanylate cyclase"
                     /protein_id="WP_078577904.1"
                     /transl_table=11
                     /translation="MDINIEQKLNKLLEGIDEKYRDKHYTLAFVDIDKCKPEKGALEEI
                     TRTFLEVVKDTLPCDAYYVGRDEFILILEGCKSDKALNLLMDLKHAWKNSEKNTFHLTF
                     SAGIASNSEHGDHASQVLTGAEEGLYRAKQEGRNMIMQPEKDQKIMKSNYYYPYQLKRL
                     KMYAEKTNNKEADILRLALNEFFRRHDEL"
     gene            complement(5001..5897)
                     /locus_tag="BK581_RS09270"
     CDS             complement(5001..5897)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) phosphonates-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474429.1"
                     /locus_tag="BK581_RS09270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_078577905.1"
                     /sec_met_domain="phosphonates-like (E-value: 2.1e-120,
                     bitscore: 398.7, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 2e-102, bitscore:
                     340.0, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 8.7e-69, bitscore:
                     229.5, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPRHLFRHYLNNPDKLIRTIGAHDAISAKIAEKNGFEAIWASGF
                     EISASHGKPDANILTMTEFLRAAEMMVEAVNIPVIADCDSGFGNVNNVIEMVKKYEKAG
                     ITGICLEDKLFPKVNSFINKRQKLADKHEFAAKIKAAKNTQVCDDFVVIARVEALIAGL
                     SIEEAIERAEAYEEAGADMILIHSKKRTSYEIEEFRGRWKSNTPIVIVPTTYPSLTEVQ
                     IKQLKIKMVIYANQGLRSAIKAMNNTLFKLGRSGSLEQINNDLESMESIFELQGMNNML
                     LNEEKYDNYQAKQLSKT"
     gene            complement(5887..6768)
                     /locus_tag="BK581_RS09275"
     CDS             complement(5887..6768)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015411.5"
                     /locus_tag="BK581_RS09275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_078577906.1"
                     /transl_table=11
                     /translation="MKVGWIGTGSLGKAAVMKMLREGERVRVFNRTAEKAVSLVKEGAI
                     LYDSPSHLALDCDIIFLCLTGHQALHDVLYEEGGILQGQKRNLIIVDISTLSPQITRET
                     AHYLSEYNIHYLDCPVSGGPEGALAGKLTAIISGNNEAYFQAERYISLFSNKKYYVGDS
                     GSSQTLKILNNLAEAINLLAASEVIALGLKEGFSLPTLKEVLTETRGNSIYMNILLERL
                     MNPSKEVSASLEVRVKDLKLANELAEKNNLELLQGKLALQQFFSAERNIGKQNDQSECI
                     KLYLKEVDINET"
     gene            complement(6765..7406)
                     /locus_tag="BK581_RS09280"
     CDS             complement(6765..7406)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014299.5"
                     /locus_tag="BK581_RS09280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_078577907.1"
                     /transl_table=11
                     /translation="MLIAFAGTDGSGKSTQVKEVKNRLLKEGYKVQILDKWDILKSEKF
                     PECKFIDTSLNDLRVNIAEMQGISRALFLFWSITITLTKDQLENEDTIYLLDGYWMKHA
                     ASEIEYGCDERWIELTCKQFPKADLTFYFDVPPEIALKRKPELTPYECGLAMDLNKTKF
                     INHQTNLRNRLLNWADTYRWLKISSLEPKEKITNFVVEKISQVTREKNLI"
     gene            complement(7406..8218)
                     /locus_tag="BK581_RS09285"
     CDS             complement(7406..8218)
                     /EC_number="2.7.4.26"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /locus_tag="BK581_RS09285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isopentenyl phosphate kinase"
                     /protein_id="WP_078577908.1"
                     /transl_table=11
                     /translation="MYVVKIGGSLITDKNAYCKPEFENIYQYAKIIKKNWTALKGNLII
                     VLGGGSYGNAVPKRYHIDNSMNHWKPENIAMMTIKMQEWMREFYYAFKKYDIPCYPFQA
                     SAFVVSNEGEHYSSYIDPIKKCLEMGLLPIISGDLVFDEKNDFGIFSSDKIPEVIAEHL
                     DVKKVVMLTDVPGVYLQNNKKEIIKTINKQNYRSVLGETGGSLKQDVTGGMKTKVKSLF
                     KLSQYGCDSVICDGREPENIIEAFNEDKQIGTLIEGGNTNNIMRGDII"
     gene            complement(8291..8839)
                     /locus_tag="BK581_RS09290"
     CDS             complement(8291..8839)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014376.5"
                     /locus_tag="BK581_RS09290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cupin domain-containing protein"
                     /protein_id="WP_169837646.1"
                     /transl_table=11
                     /translation="MITISKDHLQDLSEGRKMLINEVVKEFKEENGQIVFPSFEKVKET
                     AEFLNIPLASFFDEENSDLESGVKILRDGEGFSRTSLKNGNKYYTYNHLVTTNTEPSLM
                     PLRVELHVKDEDHVTLNGGHGSKEMVYITKGVVRMHWEHNKEQKSIDLNKGDSVYIKPG
                     VPHSFISAQDDSELLAFNY"
     gene            complement(8861..9619)
                     /locus_tag="BK581_RS09295"
     CDS             complement(8861..9619)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK581_RS09295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_078577910.1"
                     /transl_table=11
                     /translation="MDRFSHIALAKKLLETAGERPELAYMSILPLVDGEPSFLHRLHSH
                     PLVKGPVVVDCAKVVFGTIKGHVPTLNTDTFELRRFEQEKQQFIDAFNEAIGTDKEMAI
                     DDGYGALLSVISHSYFDTYNNAVQAFAPYESYCAGQYEMWSKVDYFNYRIKWYQETAPS
                     VRNKVVSEKFWDNYSFTAEELVKGMVNRVASFTRPRVSEETIKRVEHDLGVSHLPYNKK
                     VEEFYLNLEETLEKHLIESVNETENIVVQL"
     gene            complement(9644..10795)
                     /locus_tag="BK581_RS09300"
     CDS             complement(9644..10795)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012885.5"
                     /locus_tag="BK581_RS09300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LeuA family protein"
                     /protein_id="WP_078577911.1"
                     /sec_met_domain="HMGL-like (E-value: 4.4e-74, bitscore:
                     247.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKRFITIEDTTLREGEQTPGVAFNEKQKRTIVEKLADANVKFIE
                     VGIPIMGSLEYQTIKNLASDPNVPTLIGWNRGRKEDLDKTLEAGLKALHIGLPSSDIHI
                     KEKFNKSYDWVIETAIELVEYAKKQGAEFISVSAEDMGRANSEFLKRYARALAKVGASR
                     MRLSDTVGCLMPSQVENIVKMLKKEVPEDFDFQVHMHNDFNLALANVLAGVDAGASQVH
                     VTINGLGDRAGLASFHQVIPALESLKGYQTDINMKKIPEISRLVSEYSKLPVAGNEPIV
                     GEHVFTHESGIHVDGMLKVKESFEAFDPEMLGRKHEFVLGKHSGSAAIQYILAQEGLDI
                     SRDIAKDIIPAIREVSTILGTHVSPSLAVSLAKNVMKSKSLVV"
     misc_feature    complement(10814..10828)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.04"
                     /tool="antismash"
     misc_feature    complement(10935..10949)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.34"
                     /tool="antismash"
     gene            10966..11886
                     /locus_tag="BK581_RS09305"
     CDS             10966..11886
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037898.5"
                     /locus_tag="BK581_RS09305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_078577912.1"
                     /transl_table=11
                     /translation="MKATDLVKIGESIRYYRKFRKLTQKELAEDICTQAQISKMEKGEV
                     LPLSSTLFLISIKLGIKVNDLFYESAYTRYDYMKTLTRVIRTKITNHEYADVLQIIDLE
                     KDNYFNSTEMTQFILWHKGIGIAHVHKDYQHSIDLLKESLSMTNKKNGFCSERELEIIN
                     SLAIVYDLSGNSKEAHKYYKRALKESTILPSLDPNIKARMLYGLAKIEKSLGNLEKSIE
                     VSEEGRLFCINNGLLYLLGEFLYQKGYCMILLEEKTGDEYVNLAIMIFKLQGNYHLAEL
                     AERNLNEALQKVEHKKEKGLNKRLS"
     gene            complement(11932..12675)
                     /gene="map"
                     /locus_tag="BK581_RS09310"
     CDS             complement(11932..12675)
                     /EC_number="3.4.11.18"
                     /GO_function="GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene="map"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1172: methionine aminopeptidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /locus_tag="BK581_RS09310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I methionyl aminopeptidase"
                     /protein_id="WP_078577913.1"
                     /transl_table=11
                     /translation="MQKKTHKEIDRIQKSGNIIKECHEELHHFIKPGITTLDIDSYVES
                     FIRKNKAYPSLKGFNNYPFATCCSVNQSVAHEPPSKRTLKEGDIVSVDIVANVEGYHAD
                     RCFTYSVGEVDSEMKHLINVADTALKIGVSQCKEGRKISEVGQEIEDYVLKHQFSIVYQ
                     LGGHGIGKAIHEKPFFPHLGAIEEEWDFFLEEGMVLTIEPMLNIGLPEIVLKEDGWTYE
                     TIDRKNSAHFEHTILIKDDCAEVLT"
     gene            complement(12693..12824)
                     /locus_tag="BK581_RS20440"
     CDS             complement(12693..12824)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK581_RS20440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_276327422.1"
                     /transl_table=11
                     /translation="MGKNILKVCLMGLAITFGTLTISFTDHPHEASEYKASDLPHVH"
     gene            complement(12842..14005)
                     /locus_tag="BK581_RS09315"
     CDS             complement(12842..14005)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_5"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF026257.5"
                     /locus_tag="BK581_RS09315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_169837648.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 3.6e-10, bitscore:
                     37.6, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDHDLYNMAVNGVSLKIPIDMKQLKFHKLDAIYLKHYQDVEIDGE
                     SWCSFLTSETKRKNELLKVVEVLNHKKIDYLLLKGLSFQKYYPSTLYRYSLDFDFSVSN
                     FEEFLKCADTLFSRGFEYESYPQFSYNQEMRGVIKLIKYLDGGDTLSLEFNIGGFPASD
                     FTWIDYSIISKDSRTMYKNEVAINIPSHIANFIIFLAEINERDVLRVRDALDFKYMIEY
                     IKIEKVSTTLKSLYLYKNVRRVSKYLENLDGGKQENQWFRNIRRKYHTLFLDKNHIIPM
                     IYRNHYTPLRDLSMNYYKRVGEVLIDNDFFINNIKKFELLLGVRKRFYAGILTHFIPIN
                     HDKYDSFQWLTEENMDLVETPLGLFLASNFCVHTEVELEKVEELTNF"
     gene            complement(13995..14915)
                     /locus_tag="BK581_RS09320"
     CDS             complement(13995..14915)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025054.5"
                     /locus_tag="BK581_RS09320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_169837649.1"
                     /transl_table=11
                     /translation="MEKIKDINYFITEDWHLQKQSLLYADHTVRDFKGEWYFSKGHIEF
                     LISYIEGVNDFSGLTEGYIEIYTKYDNDTFKCDFSRRLENFITMKLATVDVVYIEMAIP
                     NGSKQSDVNKILKNNSNLSLYTTLVEKSIKKMPMHKTAMRDIECRAIMNEDVEDVLTCL
                     KKAYSVGLLSELRNEINVHNFNENIQDFYKQNLLSSAMISGVVTYKNKFAGHVTFSKNE
                     EESEATLVDLFIIDEYKGLGLGKELMEWGGRECLNNGIKTLKGTVDTNLSNQDPLVSNL
                     MRDNWKISKFVFLVNGKEGNLRCGS"
     gene            complement(15424..17010)
                     /locus_tag="BK581_RS09325"
     CDS             complement(15424..17010)
                     /EC_number="4.2.1.53"
                     /GO_function="GO:0050151 - oleate hydratase activity
                     [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /GO_process="GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424473.1"
                     /locus_tag="BK581_RS09325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="oleate hydratase"
                     /protein_id="WP_078577916.1"
                     /transl_table=11
                     /translation="MTKRNDERHVYFVGGGLASLAGAAYLIRDCGFKGENIHVLEGLSV
                     LGGSNDGGGSPIKGFVCRGGRMLNEETYENFWELFSSIPSLEEEGMSVTEEILAFDDAH
                     PTHSNARLINKQGKILDVMSMGFNNEDRIALGKLLLTPEEKLDHLKISDWFGTHFFETH
                     FWYMWQTTFAFQKWSSLFEFKRYMNRMIFEYSRIHTLEGVTRTKYNQYESVILPLKTFL
                     DNHGVDFSLKCTVTDLDFANTQEITVSTIHYTTEKGEGTISLKEKDLCIVTNGCMTDCA
                     TLGDLHTPAPFNAEDPISGNLWANIAAKKQGLGNPKPFFSDPGQTNWQSFTVTLKGNKL
                     LKRIEAFSRNVPGSGALMTFKDSSWLMSIVVAHQPHFKGQTEDETIFWGYGLYTDQLGD
                     YVKKPMRECTGEEILIELLHHLHFDKDVADILADVVNVIPCIMPYIVAQFQPRAMTDRP
                     KVVPEGSTNFAMISQFVEIPEDMVFTEEYSVRAARIAVYELLGITKKICPVTPHQYDIR
                     TLFKAINTTFR"
     gene            complement(17367..17921)
                     /locus_tag="BK581_RS09330"
     CDS             complement(17367..17921)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636662.1"
                     /locus_tag="BK581_RS09330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_078577917.1"
                     /transl_table=11
                     /translation="MSHRTKKALAFSLKKFLEQTTLEKMTVKDIVKECGVNRQTFYYHF
                     HDIYELLEWLFRTEVTEVIDNKNTYETWQQGFLRVLNYIETNKKVVMNTYHSLEREHLE
                     EYLYRFVYTLMIDVINEQAKGIRVTEENKQFIADFYKYAFVGIVIEWIGEGMQECPEMI
                     SDNLSKLVEGDITKALLYLQK"
ORIGIN
        1 tcaaaatatc attttcctct aaaatttctt ctagccctaa agcatctata ttttctaaat
       61 ctgtactact tttgcaatac tcccactagt tgttactgga gagacaacta aaagcattgc
      121 aattaacaaa aataagcatt tcactttccc cttcaaaacc ctccttattt acatgagtat
      181 aataaattct acatttattg gctaaatcct gcttattttt ttatttaatc caatttaatc
      241 atttactctc aaacttgctt actagaaaag ccctcgtttt atcatcatca aactagcatt
      301 tccatcttga aacgatgtat aatttaacca cttctatctt agaaagggtt gaatgatgca
      361 cagaaacgat tattttttcc tcatataatc attctataaa tggaggtaaa ataatttgct
      421 aacagaacgc caaactgaac gattagtcat gagacaagtg aaagcagaag atgctgagag
      481 cctttttcct atctggtctg atcctcgcgt cacacagttt atgaatatca caaccttcag
      541 ccaccagaac gaggctgttg acatgattaa ttacttaaac gaacttgctg agcaacaaca
      601 ggcgctccgt ttctcaatca ttgagaaaaa atccgggcgc attattgggt catgcggcta
      661 tcatttactt gatctaaaaa acgaaaaaac cgagattggt tatgaactcg cttaccctta
      721 ctggagaaaa ggttatggca ctgaggtggt caaagattta atcgatgccg ccttcttaaa
      781 actcaacctt catcgtattg aagcaaaagt tgaaccaaac aacgtgaatt caattcgact
      841 tttagaaaag gtgaacttcc tatacgaggg aaccttgagg gaaagtgaaa aatctggtga
      901 cacttatagt gatctccaac tctattcttt attgaaaact gatagtcgct aactgtatct
      961 cctattttaa aaaaggatga ctcatatgtt taacgaagct gtcatcatat caagggttta
     1021 gcaaccacaa acatcgacta tcagatggcg gctcaaagcc gcctgatcaa aagcccctac
     1081 ctacgcttag gtgggggcta taacttttaa aatacttaag aatcagggag aagggctaaa
     1141 gcgctcactg attgaaggtt cgttctattc tattaatctt ttttaagctg ctttacatac
     1201 ttgggctatt ttggtgtgta atcgcgaaat atatgcccat aaaaatattg tacgtccaaa
     1261 actaaacacg ataaatgcta gccacaatcc atgattttgc catgaaggaa atgtcagcgc
     1321 atatacggca aaaaataaaa ttaacgatac tatcattgag tttcgaatgg gggctccttc
     1381 tgtagctccg gtaaacatcc catacaataa taatccccaa aacgttacta aaggatataa
     1441 caatatccaa aaataatacg ttaacgcgag atcttttaca ctgtcaattg ttgtgaaaag
     1501 tgctagaaat ggctctttaa atacgaacag taggatcacc atcaccacgg acagacctaa
     1561 cccccaaata aaagacaagg cttttgttcg tttaaacaac tcatagttag attgtccgaa
     1621 cgcccttcct gttaaaatac tgttagcgcc agcaagtccg tcatacatgt atgccaataa
     1681 ataatgaatt tgaattaaaa tagcattggc tgcaagtatc tccacaccca attccgaacc
     1741 ttttgcagtg aaaaagccca acatagataa taaacatagt gtccgaataa ataaatctct
     1801 gttcattttt aacatagaaa caaacttttc tttttgtaaa aaagcattgg tacgccaatt
     1861 ttcaaatgag aagatagcca gtttgtttaa tacgaaaaga ccgataatga cggtgctcac
     1921 ttcagcgaat aatgtcgcaa atgccacccc tttaactccg agattgagac caagaacaag
     1981 aaaaacacta agaaaaatat tgcttatatt catgtaaagt tgcagagcaa gtgataactt
     2041 cacccttgct gaaccaatta accagccaag cacacaataa tttaataatg caaatggtgc
     2101 gccccaaatt ctaattgaga aatagtctga tgccaactgt tttacagaag gtgttgtgtc
     2161 aattagcaaa aaagcactct tttcaattgg aatctgtaaa atgataaata gtaagccaaa
     2221 gcttactgct aaaagggccg gccgaacaaa cgcagctatt aactcctgag gttgtttcat
     2281 gccacttgct tgagcagtga atccagacgt tcccactttc aaaaatccaa gtaaccaata
     2341 aatcgtatta aatattaatg tgcctattgc aacgccacct atataagtag gatcaggcat
     2401 gcgacctacg acagctgtat caaccgcccc tagaagcggt gttgtaactg ctgatagtgt
     2461 gataggaata actaaaaaaa gatactgttt agttgttata ctcgtttgat caagcgctgg
     2521 ttttctcata acttttactc attaccttta cctttcactt ttatatcatt gtctttgatg
     2581 atggcaaccg atgtctaaaa cagaggtaag taacttttta gaataacgat gtgtcagatg
     2641 gggcaaattt tccatttgat cgattgattc cactacaaga ccatttttca taaacatcac
     2701 ccgatcacaa atatacgtca cggctgttaa atcatgcgtg atgaatacat atgataaatg
     2761 atactctttc tttagctcaa tgagaagatc caatatttga agttgggtcg tcacatctag
     2821 agagcttatt gcttcatcta ataaaataac ttttggatgg gtcgccccag cccttgcaat
     2881 tcaattccat tgtaactgcc ccccactcag ttcgtgtgtg gggggtacta gtcatatgat
     2941 gtgagcttaa accaacttca ttaagcaaat gtggtttttt ctcacatgtc acttatggat
     3001 atttttctgt cgtgtgcaaa agcccagtta tccataggat acactgtaaa tctagtattt
     3061 acttgtgacg caatctagag agacacccta cttctccagc tacagcttta caaacacaat
     3121 tatttaagat ctgttaaacg tgttcttaaa gccaatatgg ctatgattaa caaaaacatt
     3181 gaggtcataa gaaaaatgag atttacctct attatgtcgg caagataggt aatgactaaa
     3241 aaagaaaatg gcagagctag cttcatgatc attgaagatg tcccaatgac tcttcctaaa
     3301 aggtgcgatg gggtgctttc ttgtctcaaa gcaaaataat gcacattaca gatagtcgca
     3361 ctaaatccta caaaaaacat actcatacaa ataacatacc aattagaaga cagaaaaata
     3421 gcaaattgag ctaggctgac agtaaaagta gccaggataa atatgtaccc tctatttaca
     3481 taatctttac tatatttagc tattagtgtt gcaacaacgc caccaatagc tgaaatacta
     3541 aaaatcaagc ctaattgtcc ttcagtgata ccaataacat caagtgcata aaatattaga
     3601 atcgtactag tagacactga tgctatatta tttcctaaaa ttaataatgt ggcactgaac
     3661 aaagctttac ttccataaag ttgtgtccat ccctctataa tatctttttt taagttaaaa
     3721 tgggatcgcg gctcatagga aggaagcttt acaggtaata cacaaactaa taaaacgatt
     3781 aatccaagca aagtaatact caggcctaca acataagtcg tataaagtat aattgcccca
     3841 gccatagcag gtcctattga attaaaaatc gtattggtaa acgtaatata agcattagcg
     3901 gtagtcagct ggctcttatc aactatagaa gggataattg attgataagt attcgcaaaa
     3961 gcataaatac acgtgtaaat aaaaaagcct agtaaatata gatgccatag ttgaatagtt
     4021 gaggtagcaa gaagtaagat gatgaatata acggaaaaaa ttttaatcaa aattgataac
     4081 agcatgattc tctttcgatt gaaccaatca atgagaaccc caataagaat ccctaaaacg
     4141 acattaggta taaactcgat cgctttcatt gttcccatgt aaaaagatga ggatgtcaaa
     4201 tcataaatga tcaaagggat acttaaagca aatatatgga atgtaaaatt gtgtgcagaa
     4261 tgaccaagca ataaaaataa gaaattctta tttttccaaa cttgcttttc ttttaatttt
     4321 gttttttctt ctaagtttgc taaactcaca ttttgctccc cctaaaaagg cggtattctt
     4381 ctaccgccta aagttcatcg tgacgtctaa aaaactcatt taaagcaagc ctaagaatat
     4441 cagcttcctt attattcgtc ttttcagcat acatttttaa gcgtttcagc tggtaagggt
     4501 aatagtaatt acttttcatt attttttgat ctttttctgg ctgcataatc atatttctac
     4561 cttcctgttt agcacggtaa agaccctctt cagcacctgt tagaacttga cttgcatgat
     4621 caccatgttc tgaattagaa gcaattcctg cactaaatgt tagatgaaac gtgttcttct
     4681 cactattttt ccatgcatgc ttcaagtcca ttaacagatt aagtgcttta tcacttttgc
     4741 agccctctaa aataaggata aattcatctc ttcctacata gtatgcatcg caagggagcg
     4801 tatctttaac tacctctaaa aatgttcgtg taatctcctc taaagctcct ttttcaggtt
     4861 tgcatttgtc tatatcaaca aatgctaatg tgtagtgctt atctctgtac ttctcatcaa
     4921 taccttctaa caacttattt aatttctgtt caatattaat atccatatga tcattatata
     4981 cataaacata tatatgtcaa ttaggtttta gatagttgtt tcgcttggta attatcatat
     5041 ttctcttcat ttaataacat gttattcatc ccttgcaatt caaaaatgct ttccattgat
     5101 tctaaatcat tatttatttg ttctaaagat cctgaacgcc ctagcttgaa tagtgtatta
     5161 ttcatcgctt taatagcaga gcgtaacccc tgattagcgt aaattaccat tttaatcttt
     5221 aattgtttta tttgcacttc tgttaaagag ggataagtcg ttggaacaat gactataggc
     5281 gtgtttgatt tccacctccc cctaaattct tcaatttcat atgaagtcct ttttttcgaa
     5341 tggataagaa tcatatctgc cccagcttcc tcatatgctt ccgctctttc aatagcctct
     5401 tctatgctca aacctgcaat caaagcttcc actctagcaa tgactacaaa atcatcacat
     5461 acttgggtat ttttagccgc ttttattttc gccgcaaatt catgtttgtc ggctaatttt
     5521 tgtctcttat taataaaact attaacttta ggaaatagtt tatcttcaag acatatccct
     5581 gtgataccag ccttctcgta ctttttcacc atctctataa cgttattaac attaccaaac
     5641 cctgagtcac agtctgctat aactggaata tttactgctt ctaccatcat ttcagcagct
     5701 cggagaaact ccgtcatcgt tagtatgttg gcatctggct taccatgact agcagatatc
     5761 tcaaatccac tagcccatat cgcttcaaat ccattctttt ccgctatttt ggcactgata
     5821 gcatcatgag caccaattgt acgaataagc ttatctggat tatttaaata atgtctaaaa
     5881 aggtgtctag gtttcattta tatcaacttc tttcaaataa agtttgatac actcagactg
     5941 gtcattttgt ttaccgatat ttctttcagc actgaagaac tgttgcaaag ccaattttcc
     6001 ttgtaaaagt tccagattat ttttttctgc taattcattt gccagtttta agtcttttac
     6061 acgcacttct aaagaggctg atacttcttt gctagggttc atgaggcgct ctaaaagtat
     6121 attcatatag atagagttcc cacgtgtctc tgttaatact tcctttaaag tgggcaaact
     6181 gaatccttct tttaacccta gggcaatgac ttctgaagct gctaataaat taattgcttc
     6241 cgctaaattg ttaagtattt ttaacgtttg ggaagagccg gaatcaccta cataatactt
     6301 tttattcgaa aaaagagaaa tatacctttc tgcttgaaaa taagcctcat tatttccaga
     6361 aataatcgca gttaatttac cagctaaagc tccttctggt cccccagaaa caggacaatc
     6421 caaataatgg atgttatatt cacttaaata gtgagctgtt tctcgagtta tttgtgggga
     6481 tagcgtacta atatcaacaa tgattaaatt tcttttttgt ccttgcaata taccaccttc
     6541 ttcatacaaa acatcgtgta atgcttgatg tcctgtcaag cagagaaaga taatatcaca
     6601 gtctaaagca aggtgtgaag gagagtcata taatatagcc ccttccttta ctaaagatac
     6661 tgctttctca gcagttctat taaaaactct tactctttca ccttcacgta acattttcat
     6721 cacagctgct tttcctaagc ttcccgttcc aatccagcca actttcatat taaatttttc
     6781 tccctcgtta cttgtgatat tttctcaacc acaaagttgg tgattttttc ttttggttct
     6841 aaggatgata tttttagcca ccgataagta tcggcccaat ttaaaagcct atttcttaag
     6901 ttagtttggt gatttataaa cttagtctta tttaaatcca tagctagtcc acactcataa
     6961 ggggtcaact caggtttacg ttttaatgct atttcaggtg gaacatcaaa ataaaaagtc
     7021 aaatctgcct taggaaactg tttacacgtt agctctatcc atctttcatc acacccatat
     7081 tcgatttcgg aagccgcgtg tttcatccaa tatccatcga gtaaataaat cgtatcctca
     7141 ttttctaact gatcctttgt taatgtgatg gtaatactcc agaataaaaa taatgctcta
     7201 cttattcctt gcatttctgc aatattaact ctcaaatcat taagtgatgt atctataaat
     7261 ttacattcag gaaatttttc cgattttaaa atatcccatt tatcgagaat ttggacttta
     7321 tacccttctt ttaatagcct gtttttaact tcttttactt gtgtactctt tcctgatcca
     7381 tcagtccctg caaaggctat tagcattaaa tgatgtctcc tctcattatg ttatttgtgt
     7441 tacctccctc aattaaggta ccaatttgct tatcctcatt gaatgcctca ataatatttt
     7501 ctggttctct tccatcacaa ataacgctgt cacaaccata ttgcgataac ttaaaaaggg
     7561 actttacctt tgttttcatt cctccggtta catcttgttt caacgatccg cccgtttctc
     7621 caagtactga acgataattt tgtttattta tcgtctttat aatctctttt ttattatttt
     7681 ggaggtagac tcctggtaca tctgttaaca tgacaacttt tttcacatct aagtgctctg
     7741 ctataacttc tggtatttta tcacttgaga aaataccaaa gtcatttttc tcgtcaaaca
     7801 cgagatctcc tgaaataatt ggaagtaacc ccatttctaa acacttttta atagggtcaa
     7861 tatatgaaga ataatgctcc ccttcattgg aaaccacaaa agcacttgct tggaatggat
     7921 aacatggaat atcatatttt ttaaaagcat agtaaaattc tctcatccac tcttgcatct
     7981 ttatcgtcat catcgctata ttttcaggtt tccaatggtt catactgtta tctatgtgat
     8041 accttttagg aacggcatta ccatatgagc ccccacctaa aacgataatt aggttacctt
     8101 tcaaagcagt ccaatttttt tttataattt tggcatattg atagatattt tcgaattcag
     8161 gcttacaata agcattttta tctgtgatca aagagcctcc aattttaaca acgtacattg
     8221 atacatcact tcctctagtt aatagatgaa attaaagcta tggaaatcca tagctttaat
     8281 tcaaaaaatc ctaataatta aaagccaaca attctgagtc atcttgagct gaaataaaac
     8341 tatgaggaac tcctggtttt atatacacag aatcaccttt atttaaatca atgctttttt
     8401 gctctttatt atgttcccag tgcattctca caacaccttt tgtaatgtag accatttctt
     8461 tagagccatg tcccccattt aaagtaacat gatcttcatc tttcacatgt aactcaacac
     8521 gtaaaggcat taaactaggt tccgtatttg tcgtaactaa atgattatag gtgtaatatt
     8581 tattaccgtt cttcagactc gttcgtgaaa agccttctcc atctcttaaa atctttacgc
     8641 cactctctaa atcgctgttc tcttcatcaa aaaatgaagc aagaggaatg tttaagaatt
     8701 cagctgtttc tttcactttt tcaaacgaag gaaagactat ttgaccattc tcttctttaa
     8761 attctttaac cacttcatta attaacattt ttcttccttc acttaggtct tgtaaatggt
     8821 ctttactaat tgtaatcatc atcatcacct catataattt ttatagttgt acaacgatat
     8881 tctcagtttc attaaccgac tcaattaagt gcttttctaa cgtttcttcc agattcaaat
     8941 agaattcttc gacttttttg ttgtatggta aatgagagac tcctaaatca tgttcaactc
     9001 tttttattgt ttcttcagat actcttggtc gcgtaaagct agctactcga tttaccatac
     9061 ctttaactaa ttcttcggct gtaaaagaat agttgtccca aaatttctct gacaccactt
     9121 tatttcttac tgagggtgct gtttcttgat accacttaat acgatagttg aaataatcta
     9181 ctttactcca catctcatat tggcctgcac agtaactttc atacggtgca aatgcttgaa
     9241 cagcattgtt atacgtgtcg aaatatgaat gtgagataac agataatagt gcgccatagc
     9301 catcatcaat tgccatttct ttatccgtcc ctattgcctc attaaaagca tcaataaatt
     9361 gttgcttttc ttgttcaaat ctccttaatt caaacgtatc tgtattaaga gtaggtacat
     9421 gaccctttat cgttccaaaa actacttttg cacaatcaac caccactggc ccttttacta
     9481 gtggatgact atgcaaccta tgaaggaaac ttggctcacc atccactaat ggaagaattg
     9541 acatataggc taattctggc ctctctccag ctgtctctaa caatttttta gctaatgcga
     9601 tgtgcgaaaa acgatccatc ttgtcaccac tcctttatat gttttataca actaatgatt
     9661 ttgatttcat tacatttttt gctagtgata cagctagaga tggacttaca tgtgttccta
     9721 aaatagtaga gacttctctt attgctggaa ttatgtcttt tgcaatgtct cttgatatat
     9781 caagcccttc ttgagctaaa atatattgaa tggctgctga accactgtgc ttacctaaaa
     9841 caaactcatg tttacgccct aacatttcag gatcaaaagc ttcaaaactt tcctttacct
     9901 ttaacatgcc gtcaacatga ataccggatt catgagtaaa cacatgttca cctactatcg
     9961 gttcgttccc agctacagga agtttgctat attctgaaac tagtcgagaa atttcaggta
    10021 tttttttcat atttatatct gtttgatacc ccttcaaact ttctaatgct ggaatgactt
    10081 gatgaaaaga agctaagccc gctctatcac caagtccatt aatggtgaca tgtacttggc
    10141 tagctcccgc atcaactcct gccaaaacgt tagctagtgc gagattaaaa tcattgtgca
    10201 tgtgaacttg aaagtcaaaa tcttcaggta cttctttctt aagcatttta acgatattct
    10261 caacttggga aggcattaaa caccctactg tatcagataa tctcatacgc gaagctccta
    10321 ctttagccag ggctcttgca tacctcttta aaaactcact gttagctctt cccatatctt
    10381 cagcactaac tgaaataaat tccgcgcctt gtttttttgc atattccacc agttctatcg
    10441 ctgtttcgat aacccaatca taacttttat taaatttttc cttgatgtga atatctgacg
    10501 agggtaaccc tatgtgtaaa gcttttaacc ctgcttctag agtcttatcc agatcttcct
    10561 ttctccctct gttccaccca atcagcgtcg gaacatttgg atcagatgcg agatttttaa
    10621 ttgtttgata ctctaaagaa cccattattg gtataccaac ttcaataaac ttcacattgg
    10681 catctgctaa cttttcaaca attgttcttt tttgcttttc attaaaagct actcctgggg
    10741 tttgctcacc ttctcttaaa gttgtatctt caattgtgat aaatcgcttt ttcatacaca
    10801 ccaccctttc gtcaattttc ttaatattat attaatatca attttcttgc atttctgaaa
    10861 ataaagagtt taaattataa tataagtaaa taacatcaaa atagtgctaa aatctctttt
    10921 tttattcttt taggaatttt ctaaaaatag gagggtgatt ttgaggtgaa agcgacagat
    10981 ttagtgaaaa ttggtgaatc gatccgatac tatcgtaagt tcagaaagct cactcaaaaa
    11041 gagcttgccg aagatatctg tactcaagcc caaataagta aaatggaaaa aggagaagtg
    11101 cttcccttat cctcaacact atttctcatc tccattaaat taggtattaa agttaatgat
    11161 ctcttttatg aatctgctta tacgaggtat gattatatga aaaccctcac gagagttatt
    11221 agaactaaaa ttaccaatca tgaatatgca gacgttcttc aaataattga cttagaaaag
    11281 gacaactatt tcaactcaac tgaaatgaca caattcattt tatggcacaa gggtattggc
    11341 attgcacacg tacataaaga ttatcaacat agtatagacc ttttaaaaga atcattaagt
    11401 atgactaata aaaagaatgg attttgctca gaaagagagt tagaaattat aaatagctta
    11461 gcaattgttt atgatttgtc tggaaatagc aaggaagcac ataaatatta caaaagagct
    11521 ttaaaagaaa gcacaatttt accaagtcta gaccctaata ttaaggctag aatgttatat
    11581 ggattagcca aaatagaaaa aagccttggg aatttagaaa agtcaataga ggttagtgag
    11641 gaagggagat tattttgtat aaataatggt ctattatatc tcttaggtga atttctatac
    11701 caaaaagggt attgtatgat tttactagag gaaaaaacag gagatgaata tgttaacctt
    11761 gctataatga tttttaaatt acaaggtaat tatcacttag ctgaacttgc tgaaaggaat
    11821 ttaaatgagg ctttacagaa agtagaacat aaaaaagaga aaggattaaa taaacgtctc
    11881 tcctgatatc atataggaga ggcaaacctt tcgctgattt aatcctttag gttaagtaag
    11941 cacttcagca caatcgtctt ttattaaaat agtatgctca aaatgagctg aattctttct
    12001 atctattgtt tcatatgtcc aaccatcttc ttttaggact atctctggta atcctatatt
    12061 taacataggc tcaattgtta ataccatgcc ttcttcaagg aaaaaatccc actcttcctc
    12121 tattgcccct agatgaggaa agaatggttt ttcatgaata gctttgccga tcccatgccc
    12181 tcctaattga taaacaatcg aaaattgatg ctttaaaaca taatcttcta tttcttgtcc
    12241 cacttcactt atttttcttc cttctttaca ttgagacacg cctattttca gagcagtatc
    12301 agcaacattt atcaaatgct tcatttcact atcaacttca ccgacagaat aagtgaaaca
    12361 acgatcagca tgataacctt ccacattggc tacaatatcg actgaaacga tatcaccttc
    12421 ttttaatgtt cgtttactgg gaggttcatg tgcgacagac tgattaacag aacaacacgt
    12481 agcgaaaggg taattgttaa atccctttaa ggaagggtag gctttatttt ttcgaataaa
    12541 agattccaca tagctgtcta tatcgagtgt agttatccct ggcttaataa aatgatgcag
    12601 ttcttcatga cactctttta ttatattccc agatttctgt attctatcaa tttctttatg
    12661 tgtttttttc tgcattaaat ttctccacct atttagtgaa catgaggcag atcactagct
    12721 ttatattctg atgcctcatg agggtgatcg gtaaaagaaa tagttagtgt cccaaaagta
    12781 atagctaacc ccattaaaca aaccttaaga atattttttc ccatttttat tccctcctgt
    12841 tttaaaaatt tgttagttct tctacttttt ccaattctac ttccgtatga acacagaaat
    12901 tactagccaa aaaaaggcct aaaggtgttt ctacaaggtc catattctcc tcagttaacc
    12961 actgaaaaga atcatactta tcgtgattaa ttggaataaa gtgagttaat attcctgcat
    13021 aaaacctctt tctaacacct agtaacaact cgaatttttt aatattattt ataaaaaaat
    13081 cattatcaat caatacttct ccaactcttt tgtagtaatt catcgacaaa tctcttagag
    13141 gcgtgtaatg attcctatat atcattggta ttatatgatt cttatctaga aataatgtgt
    13201 gatattttct tcttatattt ctaaaccatt gattctcttg ctttccgcca tcaaggtttt
    13261 ctaaatactt agagactctt cgaacatttt tatacaaata aagggatttt aaagtggtgg
    13321 acactttttc gatttttata tactcaatca tatacttgaa atctaaggca tctctaactc
    13381 gtaacacatc cctttcatta atttctgcca aaaatataat aaaattagcg atatgggaag
    13441 gtatattaat tgctacttca tttttataca ttgttctcga atctttagaa atgatcgaat
    13501 aatcaatcca agtaaaatca cttgctggaa atcctccaat attaaattca agtgatagtg
    13561 tatccccacc atccaagtat ttaataagtt ttattactcc tctcatttcc tgattataac
    13621 taaactgagg atatgactca tattcaaaac ccctcgaaaa taaggtgtct gcacacttca
    13681 gaaattcttc aaaatttgac acactaaagt caaaatctag actataccta tataatgtac
    13741 taggatagta tttttgaaat gagagtccct ttaataataa ataatctatt tttttgtgat
    13801 ttagtacctc tactaccttc aatagctcat tttttctttt agtttctgaa gtaagaaagg
    13861 agcaccagct ttccccgtct atctcaacgt cttggtaatg ttttaaatat atggcatcta
    13921 gcttatgaaa ttttaattgt ttcatatcaa ttgggatttt aagagacaca ccatttacgg
    13981 ccatattgta aaggtcatga tccacaacgt aaattcccct cctttccatt aactaaaaat
    14041 acaaatttag agattttcca attatctctc attagattcg atactaaagg atcttggtta
    14101 cttaaattag tatccacagt ccctttgagc gtttttattc cattatttaa gcattccctt
    14161 cctccccact ccatcagttc tttacctaac ccaagtcctt tatattcatc aattataaat
    14221 agatctacga gagtggcttc tgactcttct tcattttttg aaaaagtcac atggcctgca
    14281 aatttatttt tataagtaac cactcctgaa atcatggcag aagaaagaag gttttgttta
    14341 tagaaatctt gaatattttc attaaaatta tgcacgttta tttcatttct taattcagat
    14401 aataagccta cagaataggc tttcttcaaa caagttaaca cgtcctctac atcttcattc
    14461 ataatagctc gacactcaat atccctcatt gctgttttat gcattggcat tttttttatg
    14521 cttttttcta ccaaagttgt atatagagat aaatttgaat tgtttttaag aattttattc
    14581 acatcacttt gtttactacc gttaggaata gccatctcta tgtaaaccac atcaactgtt
    14641 gctagtttca ttgtaataaa attttctaac cttctagaaa aatcacattt aaaggtatca
    14701 ttatcatatt ttgtgtagat ttctatataa ccctcagtta aaccggaaaa gtcgttaact
    14761 ccctctatgt agctaattag gaattctata tgccccttcg aaaaatacca ttccccttta
    14821 aaatctctta ctgtatgatc cgcatataaa agagattgct tttgtaaatg ccaatcttca
    14881 gttatgaaat agtttatgtc tttaatcttt tccatcatta cccctccgtt cctagtttat
    14941 aaggaggccc tccaacagtt atattggagg gttaaagata taaattacat attcttaatg
    15001 agttacctta cttacgacgt ttaatttttt taatttgctt ataagttaca gttgcaccca
    15061 tttagctcac ctcccacttt ttttatacac accacaagac ataaatacat taacgtatat
    15121 atgtctttgt gtatatgtta atagcttttt caaataatgt caactgattt ttttaatttt
    15181 tcaaaatata ttttaaattt gtatctaaca ttttccattc cactttcatt aaccctgacg
    15241 ttaaattaag aggcgcaaat gaatcatata gctataagcc tttataaact tatctaatat
    15301 ccatttctct tcataaaaaa tttactccag gcaaaaaaac ttccctaagg gtagacataa
    15361 ttctttatac gtctactttt aggaaagcgg atcatgatca tggttccttt ttttgctgtt
    15421 tatctaccta aacgtcgtat ttatcgcttt aaataatgtt cgaatatcat actgatgagg
    15481 cgtaacaggg cagattttct tcgttatccc caacaactca taaaccgcga ttctcgcagc
    15541 acgaaccgag tactcttccg taaacaccat gtcttcagga atctcaacaa actgactaat
    15601 catcgcaaag tttgttgagc cctctggaac gacttttggt cgatcagtca tcgccctcgg
    15661 ttggaattgt gccacaatat acggcatgat acacggaatt acgttaacaa cgtcagctaa
    15721 aatatcagcc acgtctttat caaagtgaag gtgatgaagc agttctatta atatctcttc
    15781 accggtacac tctcgcatag gctttttcac ataatcccct agctgatctg tgtataatcc
    15841 atatccccaa aagatcgttt catcctcagt ttgtccttta aaatgtggct ggtgtgccac
    15901 cacaatagac atgagccagc tggagtcttt aaacgtcatt aaagcaccac ttcctggcac
    15961 attacgagaa aaagcttcta ttctcttcag cagtttgtta ccttttaacg tgacagtaaa
    16021 cgattgccag ttcgtttgtc cggggtccga aaagaaaggt ttcggattac caagcccttg
    16081 ttttttagct gcaatgttgg cccacaaatt ccctgaaata ggatcttcag cattaaatgg
    16141 ggcgggggtg tgaagatcgc ctaatgtggc gcaatctgtc atacatccat ttgtcacaat
    16201 acaaaggtct ttttctttta atgaaatcgt cccctcgcct ttttctgtcg tataatgaat
    16261 cgttgacaca gtaatctctt gcgtattagc aaaatctaag tccgtcaccg tacattttaa
    16321 tgagaaatcg accccatgat tatctaaaaa agttttgagt ggtaaaatca cggattcgta
    16381 ttgattatac ttcgttctcg ttactccttc taatgtatga atacgcgaat attcaaagat
    16441 catccgattc atatagcgct taaattcaaa taagcttgac catttttgaa aagcaaatgt
    16501 cgtttgccac atgtaccaaa aatgcgtttc aaaaaaatgc gtcccaaacc aatcggatat
    16561 ttttagatga tctagttttt cttccggtgt tagtaataat tttcctaggg ctatacgatc
    16621 ctcattattg aagcccatac tcatcacgtc taaaatctta ccttgtttat taataagacg
    16681 agcgttggaa tgtgtcgggt gtgcgtcatc aaatgctaga atctcctctg tgacactcat
    16741 gccttcttct tcaagtgatg gtattgagga aaataactcc cagaagtttt cgtatgtttc
    16801 ttcattcagc atacgtccgc ctcggcaaac aaaccctttt atcggagacc caccgccatc
    16861 atttgaccca cctagaacag atagtccttc aagaacatga atgttttctc ctttgaaccc
    16921 acaatcccta atcaagtacg cagcaccagc caatgaagcg aggcctcctc ctacaaaata
    16981 gacgtgtctc tcatcattcc tttttgtcat ggtcaattcc tccaccaata cgttttacaa
    17041 ggaaggtacc cccttgttct atatagtgtg actggattga tgttggagac atatagacaa
    17101 ttaaggcctc gtgtcaaaac tttcaacaaa aaccctcgat tgtctaacaa taagtatgat
    17161 aagagattgc tttttaattg ctctgaagaa attaaacata tttggctata atctatatgt
    17221 taagttctat ctcaaactct gtcgtatggc atttttacta tgaagtaatg atgtgtataa
    17281 aagataaata gcttgtctga agtggagaat gacaaacgag gggtgtcctt cattataaaa
    17341 tggctgttta aaagcttact ctataactat ttttgtaaat ataacagtgc tttagtgata
    17401 tcaccctcaa ctaacttact taaattgtca cttatcattt ctggacattc ttgcatacct
    17461 tcgcctatcc attctatgac aattccaaca aaagcatatt tgtaaaagtc agcaataaat
    17521 tgtttattct cttctgtgac acgtattcct tttgcttgct cgttaataac atcgatcata
    17581 agagtgtaaa caaagcgata cagatactct tctaaatgtt cacgctccaa tgaatggtat
    17641 gtgttcatca cgaccttttt gttagtttct atatagttta gaactcgtaa gaaaccttgc
    17701 tgccatgttt catatgtgtt tttattatca atgacttcgg ttacctccgt acgaaataac
    17761 cactctaata attcataaat atcgtgaaaa tggtaataaa aggtttggcg atttacgcca
    17821 cactccttca caatatcttt aacagtcatt ttctctaaag tcgtttgctc caaaaatttt
    17881 tttaaagaaa aggctaacgc tttttttgtt ctgtgagaca tacaacattc ctcctcacca
    17941 ttcttacgag cgaccttcac tactttaaaa tgtttagaca tctggatgaa aaaaatcaac
    18001 gtcggccact agagctataa atccttctta gtacagttaa tccacttaaa acgccctttc
    18061 aatcaatggg cgttttcgat cttctcccac tgagtgatag ttgatttaat caggacatta
    18121 gcgtccgtta actcccgcct aaatagcttt gctctctatt ttgagcgaag agtttgacgg
    18181 acgtttatat gtgataaaaa gctattacct atagtttact ataattggga ttgaacttat
    18241 aactacttta attgaagttt ttttataaac acaatgttgg tttccttgta aatatatcgc
    18301 tcaaggagga tcgttttact cttacgtttg gaaaaatttg atgggttttt tcataagttt
    18361 gaaaccataa aacgaatttt ttcagtggaa gttttcattc ttctacaact gattggtagt
    18421 taaatcaatc aggacttatc tgtcataaaa aagatctgtg cctttaaaac aagacacaga
    18481 cctcattcat tcaatactct attatttgct cttctactct acactttcgt actgaaaatc
    18541 atctatttct atcacaaatt ggccagtatt tatactcctc aatgtctctt ccatttcttc
    18601 cgttaacgct aagccaaaag gaatagcatg gttggattca agggtgccaa tatcctcctt
    18661 agaaagaact acgtgatgtt ggtcccagac cttcttctcg tctgtaccgt cccccatcgt
    18721 taaagagaat gaaatatctt gaaggttaaa aggcagtcga ttaacaatta aaaatacaag
    18781 ttgagtgcct tcaccaaaat cttcataaat atcaaatgaa tgaaagctca catcgttagc
    18841 cgttcctaat tcaggggtct cttcaataaa actcgttaat tcaacaagtg cgcgcgggga
    18901 gccctgtcct gccatttcta gcgttaactc cttcatttcg tcattgttat cttctccaga
    18961 gcttacggaa gagttacagc cagttataag taataaggtt attaa
//
