LOCUS       NZ_KV917378            10390 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Salipaludibacillus agaradhaerens strain DSM 8721 Scaffold1, whole
            genome shotgun sequence.
ACCESSION   NZ_KV917378
VERSION     NZ_KV917378.1
KEYWORDS    WGS; RefSeq.
SOURCE      Salipaludibacillus agaradhaerens
  ORGANISM  Salipaludibacillus agaradhaerens
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae.
REFERENCE   1  (bases 1 to 4319439)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Genome sequencing project for genomic taxonomy and phylogenomics of
            Bacillus-like bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319439)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2017) Agricultural Biological Resources Research
            Institute, Fujian Academy of Agricultural Sciences, No. 247, Wusi
            Road, Fuzhou, Fujian 350003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 227.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002019735.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 15:23:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,010
            CDSs (total)                      :: 3,909
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 101
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 17 of 38
            Pseudo Genes (incomplete)         :: 21 of 38
            Pseudo Genes (internal stop)      :: 7 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:59:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 577288
            Orig. end                         :: 587678
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV917378.1.
            Bacteria and source DNA available from DNA is available from: DSM
            or Fujian Academy of Agricultural Sciences.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5390)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    36..51
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 22.93"
                     /tool="antismash"
     misc_feature    66..77
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.15"
                     /tool="antismash"
     gene            143..619
                     /locus_tag="BK581_RS02655"
     CDS             143..619
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897183.1"
                     /locus_tag="BK581_RS02655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6884 domain-containing protein"
                     /protein_id="WP_078576703.1"
                     /transl_table=11
                     /translation="MSELCIIPCGNKKIWSKHPNLEAVPASDAYISTFHRLCRAYAERF
                     FTQYVILSAKHGFLFAEDHVPGPYNVSFSMKSSEVISTETLKKQFENKRLHNYDHYIVL
                     TGKKYVPVINHVMNDENNVEFPLLDYQGIGFMQQALKHALETNEPLHGKKIKNR"
     gene            616..1116
                     /locus_tag="BK581_RS02660"
     CDS             616..1116
                     /EC_number="3.2.2.15"
                     /GO_function="GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017796204.1"
                     /locus_tag="BK581_RS02660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="WP_078576704.1"
                     /transl_table=11
                     /translation="MKRQSMQPIVASGAKILLLGSMPGEQSLIKQQYYGNKRNHFWPII
                     SDLLNVDLTTPSYQYKCQQLIGHGIALWDVIASCEREGSLDSAIRHEKANDIKGLLQQY
                     PTIRWIGLNGTKAYQSFLKYKKKVDIPELPFTKLPSTSPVPGKNVKSYEEKVEAWREIS
                     HYL"
     gene            1134..2018
                     /locus_tag="BK581_RS02665"
     CDS             1134..2018
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017470498.1"
                     /locus_tag="BK581_RS02665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="5'-3' exonuclease H3TH domain-containing protein"
                     /protein_id="WP_078576705.1"
                     /transl_table=11
                     /translation="MSDKTLLLIDGFNLLSRGYFATAYGKSDEQLSKNDQGQYINAVRV
                     FFQKLFQLTRQYPITHISVAWDGKRQDTSRNQQYAFYKAQRQDLPEPLIEQYHLTKELL
                     DNLNVHQLAVGGYEADDIIGAFSTLWAESGFGQTYIYSNDRDLFQLLNPTTAQILSKKK
                     QEIVYSDEAFQAEYDITPTQWVDVKALLGDASDNIPGCPGVGEKSALPLIRQYGDLKQV
                     FKNVDSLDPTFNRYKKKLREGEETTWISYELSQIITDIEAVTAVNHSELEFSWDEANAL
                     HAMAAKGIKVKIR"
     gene            2265..2756
                     /locus_tag="BK581_RS20505"
     CDS             2265..2756
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013648.5"
                     /locus_tag="BK581_RS20505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_322788410.1"
                     /transl_table=11
                     /translation="MRLVSICPSNTELATYAGLVDHLVGVDNYSDWPSDKLTNLERVGP
                     DLNINMDKVEALNPDLVLASETVPGMEKNIKELKKRGLPYVIVPTPKTLSDISSQLIWI
                     GEKTGYKEKSLKAHKTFNYWINYYKGLSNSVEEKKTIYWEWWAKPIFTPGHSNWLTEMS
                     "
     gene            2757..3086
                     /locus_tag="BK581_RS20510"
     CDS             2757..3086
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK581_RS20510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_322788411.1"
                     /transl_table=11
                     /translation="MAGGINIFADNPQSSVKTTWEDVYKRNPDVMAIIWVGVHKEKVNR
                     KVLEKRPDWQSLKAPTSNNVYILDEPFFCRPSPRLLIGLMQIANILHPHIYPTFVEGVD
                     PMFTD"
     gene            complement(3083..3484)
                     /locus_tag="BK581_RS02675"
     CDS             complement(3083..3484)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499096.1"
                     /locus_tag="BK581_RS02675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="WP_078576706.1"
                     /transl_table=11
                     /translation="MEKRDIILFDGVCHLCQSSVQFIIKRDPQNHFAFASLQSEIAKNL
                     IEQKEITEESNSVVLIKTNGKSYKNSSAALRIAKELKGVWKLAAILLIIPVFIRDPIYR
                     FIARHRYTWFGKSEKCMMPTPSQRRRFLD"
     gene            complement(3776..4840)
                     /locus_tag="BK581_RS02680"
     CDS             complement(3776..4840)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016999.5"
                     /locus_tag="BK581_RS02680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AbrB family transcriptional regulator"
                     /protein_id="WP_078576707.1"
                     /transl_table=11
                     /translation="MSYQIKPFIEAIVISVIGGGLFHVVSLPLAWMLGPLTSVMLWEGL
                     TTRTLVWPDFLKKLGLIILGISFGFYFTFESLKIVGPFIIPYLLITVILILISIFNSAF
                     ITKWINIDKKTSVFGSIPGGLAEMVVASEDVKANSALVMVFQTIRLLVVLFTVPFIITQ
                     FFPHDSNAISAVMTIPDTNILSLHGLWLILPILLGIKYQHSLPAGIMIIPLAVTATISI
                     SSINIPSIPPLIFLIAQLFVGASLGKSISLTDIKLAKKYAIIYIGLACWLIVLSVGFGI
                     LLESMTTMDLQTAILSTAPGGLVEMVLTASIVGADPAIVTSLQLMRVMVIVLVAPSALK
                     WYFSRKINDSNKIA"
     misc_feature    4944..4960
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 25.58"
                     /tool="antismash"
     gene            complement(5001..5390)
                     /locus_tag="BK581_RS02685"
     CDS             complement(5001..5390)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897090.1"
                     /locus_tag="BK581_RS02685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_078576708.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.1e-52, bitscore:
                     173.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVVKLEDIIGTDQEIKGGNWTSRRLILKKDNMGYSVHDTIIKAG
                     TETHIWYKNHLEAVYCIEGDGEVETLSDNKIHPISANTLYALDEHDEHLLRANTDMRMV
                     CVFNPPITGQEVHNEEGVYELVEED"
     gene            complement(5422..6723)
                     /gene="ectB"
                     /locus_tag="BK581_RS02690"
     CDS             complement(5422..6723)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (26-362). E-value: 2.2e-77.
                     Score: 252.3. Matches aSDomain:
                     nrpspksdomains_BK581_RS02690_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013488592.1"
                     /locus_tag="BK581_RS02690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_078576709.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.2e-76, bitscore:
                     255.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKNHLEIIEEHESCVRSYVRSFPTVFTQARGYKMWNEDGKEYID
                     FFSGAGALNYGHNEPNMKKKLVEYILDDGITHSLDKATEAKSEFLHKFHEVILQPRNLD
                     YKVMFPGPTGTNTVESALKLARKVTGRTEVISFTNGFHGMTIGALSVTGNSMKRKGAGI
                     PLNHSVTMPYDQFVNESDEMDTLAYLERFLDDNGSGVSIPAAIILETVQGEGGLNAARF
                     EWLNKLDAICKKWGILLIIDDVQAGVGRTGTFFSFEPADIKPDIVCLSKSIGGYGLPLA
                     LTLINPELDKWKPGEHNGTFRGNNHAFITATEALSYWEDPKFEKSIQDKAVKITDFLTN
                     MVEKYPEMKGFVKGRGFMQGISSDIDGFSEKVCENAFNHGLIMETAGGHDQVFKLFPAI
                     NIDEEGLEKGFELIERSIKDTIKQMKLEKETVTN"
     aSDomain        complement(5638..6645)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK581_RS02690_Aminotran_3.1"
                     /evalue="2.20E-77"
                     /label="BK581_RS02690_Aminotran_3.1"
                     /locus_tag="BK581_RS02690"
                     /protein_end="362"
                     /protein_start="26"
                     /score="252.3"
                     /tool="antismash"
                     /translation="FTQARGYKMWNEDGKEYIDFFSGAGALNYGHNEPNMKKKLVEYIL
                     DDGITHSLDKATEAKSEFLHKFHEVILQPRNLDYKVMFPGPTGTNTVESALKLARKVTG
                     RTEVISFTNGFHGMTIGALSVTGNSMKRKGAGIPLNHSVTMPYDQFVNESDEMDTLAYL
                     ERFLDDNGSGVSIPAAIILETVQGEGGLNAARFEWLNKLDAICKKWGILLIIDDVQAGV
                     GRTGTFFSFEPADIKPDIVCLSKSIGGYGLPLALTLINPELDKWKPGEHNGTFRGNNHA
                     FITATEALSYWEDPKFEKSIQDKAVKITDFLTNMVEKYPEMKGFVKGRGFMQGIS"
     gene            complement(6775..7284)
                     /gene="ectA"
                     /locus_tag="BK581_RS02695"
     CDS             complement(6775..7284)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017187169.1"
                     /locus_tag="BK581_RS02695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_078576710.1"
                     /transl_table=11
                     /translation="MSKSTTAVIETFSLEQPTKEDGQAMWALAKNTTLDLNSAYKYIMM
                     AEYFSETCVVAKERGEVVGFITGFIQPEHQDVIFIWQVGVDSSQRGKGVASKMLNDLLE
                     RKACKNVRYVEATITEENKASQSLFKRLARDHDTAYEVKECFPEEIFPEEGQKAEYTYR
                     IGPFSK"
     misc_feature    complement(7286..7296)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            complement(7606..9453)
                     /gene="typA"
                     /locus_tag="BK581_RS02700"
     CDS             complement(7606..9453)
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /GO_process="GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="typA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898783.1"
                     /locus_tag="BK581_RS02700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="translational GTPase TypA"
                     /protein_id="WP_078576711.1"
                     /transl_table=11
                     /translation="MTIRSDIRNIAIIAHVDHGKTTLVDELLKQSGTFRENEQVNERAM
                     DNNDIEKERGITILAKNTAVDYEGVRVNILDTPGHADFGGEVERILKMVDGVLLVVDAF
                     EGCMPQTRFVLKKALEQKLTPVLVVNKIDRPMARPEEVVDEVLDLFIELGADEEQLEFP
                     VVYASAINGTASADSDPTKQDDNMRVLFNTVIETVPAPIDNSQEPFQFQVTMLDYNDYL
                     GRIGVGRVFRGTIRKGDQASLVKRDGSIKTFRISKLFGFLGLKRLEIDEAKAGDLIAIA
                     GIEDIMVGETICPSDNPDPMELVRIDEPTLQMTFLVNNSPFAGREGDHVTSRKIEARLL
                     TQVETDVSLRVENTSSPDIWTVSGRGELHLSILIENLRREGYELQVSKPEVIIREVDGK
                     PCEPFENAQVDVPEEYTGAVMESLGERKAELLNMVNKGDGQVRMDFLVPSRGLIGYTTE
                     FMAQTKGYGILNHTFDSYQPVAQGYVGGRRQGVLVSMENGKATPYGMLQVEDRGILFVE
                     AGTEIYEGMIVGEHNRDNDLTVNITKMKQQTNIRSATKEQTVTMKKPRLLTLEEALEFL
                     NEDEYCEVTPHSIRLRKKILDKSMREREEKRKKLSAKNN"
     misc_feature    9438..9453
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.3"
                     /tool="antismash"
ORIGIN
        1 cgtccgtaaa cctcccgctt caaattgccc gggaggttta cggatgttaa ctgtgataaa
       61 ttaatgaaaa tgctttctgt taagtgaacg aagctaatat gtgataggat agtagagtaa
      121 atgtagataa ggagcgtaaa atatgagcga attgtgtatt attccatgtg gtaacaaaaa
      181 gatttggagc aaacatccta acttagaggc agtaccagct tctgatgcct atattagcac
      241 gtttcatcgc ctctgtagag cttatgcaga gagatttttt acccaatacg ttattttatc
      301 tgctaaacac ggctttcttt ttgcagaaga tcatgttcct gggccataca atgtcagttt
      361 ctccatgaaa tcttccgaag ttatctccac agaaacgtta aaaaaacaat ttgaaaataa
      421 aagattacac aattatgatc actatatcgt tttaacagga aaaaaatatg taccagtcat
      481 taatcacgtt atgaacgatg agaacaacgt agagtttcct ttattagatt atcagggaat
      541 tggatttatg caacaagcat taaagcatgc tctagaaaca aatgaacctc ttcatggaaa
      601 gaagataaaa aaccgatgaa acgccaatcg atgcaaccaa tcgtcgcttc tggagcaaaa
      661 attcttttgc ttggttccat gcctggggag cagtctctca taaaacaaca gtactatggg
      721 aacaagagaa atcatttttg gccaatcata agtgaccttc tcaatgtcga tcttacaacg
      781 ccttcatatc agtataaatg tcaacaactt attggacatg gtattgcgtt atgggatgtg
      841 attgccagct gtgaacgaga agggagtctc gattcagcta ttcgtcatga aaaagcaaat
      901 gatattaaag gattgttgca gcaataccca acgatccgtt ggattggatt aaacgggacc
      961 aaagcctacc agtcgtttct aaaatataag aaaaaggttg atatcccaga gttaccgttt
     1021 acgaagctac catctactag tcctgttcca ggtaaaaatg tgaagtccta tgaagagaag
     1081 gtagaggctt ggcgagaaat aagtcattac ttataatttt aaaggagtac atcatgagtg
     1141 ataaaacatt gttattaatt gacggattca atttattaag taggggctat tttgcaactg
     1201 cgtacggaaa atctgatgag caattgtcta aaaatgatca aggtcagtac attaatgctg
     1261 tgcgtgtttt ctttcaaaaa ttatttcaat taacgcgaca atacccgatt acacatattt
     1321 ctgtcgcatg ggatgggaaa cgtcaggata cgtcacgtaa tcaacagtac gcgttttata
     1381 aggcgcaacg acaagattta ccggagccac tgatcgaaca gtaccactta acaaaagagt
     1441 tgctagacaa tttaaacgta catcaactag ccgtaggcgg ttatgaggcg gatgatatca
     1501 tcggtgcttt ttcgacattg tgggcagaaa gtggctttgg acaaacgtat atttatagta
     1561 acgatagaga tttatttcaa ctgctcaacc ctacgaccgc tcagattttg tctaaaaaaa
     1621 agcaggaaat tgtctatagt gatgaagcat ttcaagcaga gtacgatatt acacctactc
     1681 aatgggttga tgtaaaagcg ttgctaggag atgccagtga caatattcca ggttgtcccg
     1741 gtgtaggtga aaaatctgcg ttacccctta tccgtcagta tggcgatctt aaacaagtgt
     1801 ttaaaaatgt cgattcattg gatcctactt ttaatcgtta taagaaaaaa cttcgtgaag
     1861 gagaagaaac gacgtggatc agttacgaac tttcgcaaat tatcacagat attgaagccg
     1921 tcacggcggt gaaccattct gaacttgaat tttcatggga tgaagcgaat gcgttacacg
     1981 caatggctgc taaaggaatt aaagtaaaaa taagatgatc atgagagaga aagaagggtg
     2041 attagtgttt aacctattca ggcgggcaaa aaggtgtact ttttgtgggc gaaaaacaat
     2101 gaagccaaca agttattata acgatcatgg aaatgagctc tttgtgtgct ttcaatgtgt
     2161 gccacaagca gaaaggcgct cattacttaa gaagtgaaat tggaaaggat catcattctg
     2221 accgtcaatc acttaattaa tgctataaaa agggaggacg cttcatgcgt ttagtatcta
     2281 tttgcccgag taatacagaa cttgctacct atgcgggttt agttgatcac ctcgtcggtg
     2341 tcgataatta ttcggactgg ccttcagata aattgacgaa tctagagaga gtgggcccag
     2401 acctgaatat aaatatggat aaggtagaag cattaaatcc agatctcgtg ttagcctctg
     2461 agacggttcc tggaatggaa aaaaatatta aagagttgaa aaagagaggc cttccatatg
     2521 tgatcgttcc tacgcctaag acattatctg atattagcag tcagttaata tggatcggtg
     2581 aaaaaacagg ctataaggag aaaagtttaa aagctcacaa gacatttaat tattggatta
     2641 attactataa aggtttgagt aattccgtgg aagaaaagaa aacgatctac tgggagtggt
     2701 gggctaagcc tatttttact ccaggtcact ctaattggct aaccgaaatg tcttaattgg
     2761 caggagggat caatatattt gctgacaacc ctcaatctag tgtaaaaacg acatgggaag
     2821 acgtgtataa gcgtaatccg gacgtgatgg ctattatatg ggtcggcgtt cataaagaaa
     2881 aagttaatcg taaagtgttg gagaagcggc ctgattggca atcgttaaaa gctcccacat
     2941 caaacaatgt ctatatcctt gatgaaccat tcttttgtcg gccttcacca cgattattaa
     3001 ttgggcttat gcaaatagcg aacattctac accctcatat ttatcctacg tttgtcgaag
     3061 gcgtagaccc gatgtttacc gattagtcta gaaagcgccg gcgctgtgaa ggcgtcggca
     3121 tcatgcattt ttcagatttt ccaaaccacg tgtatctgtg acgagcaata aagcggtaga
     3181 taggatcgcg aataaaaacg gggataataa ggagaatggc tgctagtttc catactcctt
     3241 ttaactcttt tgcaattcga agggcggccg atgaattttt atatgatttt ccgtttgttt
     3301 taattaggac aacgctgtta gactcctcag ttatctcctt ttgttcgatc aaatttttgg
     3361 cgatttcact ttgtaatgaa gcaaaggcga agtgattttg cggatcgcgt tttataataa
     3421 attgaacgct tgattggcac aaatgacaaa ccccatcaaa caagataata tctctttttt
     3481 ccatttgatc aactcctttt ctttttttca aagtttatga ttaaactaat tttcacatac
     3541 aaaatacacg tgtaggaaac gacttacaca gtgatcttac gctaatgtga gatagctgat
     3601 ttatcattgt tcctattgaa aatttaattg tgatacattt ttctgtttta taaatgaaaa
     3661 agagcataat tagatagggc tagtcacttt tggagttcta attgagaaaa gaagtgttaa
     3721 gtattgtgtt agcgaattaa taagtaaaga taatataacc gtttttgtaa aacgtttaag
     3781 caattttatt gctatcgttt atttttcgag aaaaatacca ttttaacgca cttggagcaa
     3841 ctaaaacaat aaccataaca cgcattaact gtaacgacgt cacaattgct ggatcggcgc
     3901 ctacgattga ggctgttaat accatttcca ctaatcctcc tggtgctgtg ctcaatatcg
     3961 ctgtctgtaa atccattgtt gtcattgatt caagtaatat gccaaaacca accgataaaa
     4021 ctattaacca gcaagcaagc cctatgtaaa taatggcgta tttttttgca agtttaatat
     4081 ctgttaacga aatactcttg ccaagagaag cccctacgaa tagctgggcg attagaaaaa
     4141 tcaggggagg aatagaagga atattaattg agctgatact tatagtagcc gttacagcta
     4201 acgggataat cattattccc gcaggcaaag agtgctggta tttgatacct aacaaaatcg
     4261 gtaatatgag ccataatcca tgtagcgata atatgtttgt gtcaggaatc gtcattactg
     4321 ctgatatggc attgctgtca tgggggaaaa attgggtaat aataaatgga acagtgaata
     4381 aaacgactag taaacgaatt gtttgaaaaa ccatgaccaa agctgaattt gctttaacat
     4441 cttcacttgc tacaaccatt tccgctagtc caccaggaat tgaaccgaaa acactcgttt
     4501 ttttatcaat gttaatccat tttgttatga atgcactatt aaaaatactt ataagaatga
     4561 gtataactgt aatgagtaag tacggaataa taaagggtcc aactattttt aacgattcaa
     4621 acgtaaaata aaatccaaat gatattccta gaataataag gcccaatttt tttaggaaat
     4681 ccggccaaac taacgtcctt gtagtcagtc cttcccataa catgacacta gtgagcgggc
     4741 ctagcatcca agcaagtggt agagacacga cgtgaaaaag ccctccacct atcactgaaa
     4801 taacgatcgc ttcaataaac ggttttattt gataactcat tgtatttcat ctccaaataa
     4861 gtctaatctt tattgtaaca tgagaaaaga ggagagaggt gggggatgat ggataataag
     4921 gtagtattac agagaaaacc ctgctgcata caagatgcaa cagggaagtg ttagacattt
     4981 taagcagtgt ggcagatcat ttaatcttct tcaactagtt cataaacgcc ttcttcgtta
     5041 tgaacttctt gacctgtaat aggtgggtta aatacacaga ccattctcat atcggtattt
     5101 gcacgaagta agtgttcatc atgttcatct aaggcataga gtgtattggc tgaaataggg
     5161 tggattttat tgtcagacaa tgtttccact tcaccgtcac cctcaataca ataaacagct
     5221 tcgaggtgat ttttgtacca aatgtgcgtt tcagttccag ctttaatgat cgtatcatga
     5281 actgaatagc ccatattatc tttttttaga ataagtcgac gactcgtcca gttaccacct
     5341 ttaatttctt gatctgttcc aataatatct tctagtttaa caactttcat ggatgttcct
     5401 ccttgtgtgt acagttgatt attaattggt gactgtttct ttttcaagtt tcatttgttt
     5461 aatggtatct tttatgcttc gttcaatgag ttcaaaacct ttttctaatc cttcttcgtc
     5521 aatgttaata gcagggaata atttaaatac ttgatcatgt ccacctgctg tttccataat
     5581 tagcccatga ttaaaagcat tctcgcaaac tttttcacta aagccatcaa tgtcagaaga
     5641 gattccttgc ataaagcctc ttcctttaac gaaccctttc atctcaggat atttttctac
     5701 catgttagtg aggaaatctg tgattttaac tgctttatct tgaatactct tttcaaattt
     5761 cggatcttcc caataactta aagcctctgt ggcagtaata aaagcatgat tattaccacg
     5821 gaatgtcccg ttatgttcac caggcttcca cttatctaat tcaggattta ttaatgttaa
     5881 tgcaaggggg agaccatagc caccaataga tttagataag caaactatat caggtttaat
     5941 atccgcaggt tcaaacgaaa agaatgttcc tgtccggcca acaccagctt gcacgtcgtc
     6001 aataatgaga agaatacccc attttttgca tatagcatct aatttattta gccattcaaa
     6061 gcgcgctgca ttaagaccac cttcaccttg aacggtttca aggataatgg ctgcaggaat
     6121 ggagacgcca ctgccgttat catctaaaaa tctctcgagg taggcaagtg tatccatctc
     6181 atcactttcg ttaacaaact ggtcgtatgg catagtgacg gagtggttta atgggatacc
     6241 agcgcctttt cgtttcatag aattacctgt gactgacaaa gcgccaatcg tcatgccatg
     6301 aaagccattt gtaaaactga tgacttctgt tctacctgtg acttttcttg ccaactttag
     6361 ggcactttca accgtattcg ttcctgttgg tccaggaaac atgactttgt aatcaagatt
     6421 ccttggttga agtataacct catgaaattt gtgaagaaac tcactttttg cttctgttgc
     6481 tttatctaaa gagtgagtaa taccatcatc aaggatgtat tctactaatt ttttcttcat
     6541 gttaggttca ttatgtccgt agtttagagc gcctgctcca gaaaagaagt caatatattc
     6601 ttttccgtct tcattccaca tcttataacc tctagcttgg gtgaaaactg ttggaaagct
     6661 tctcacataa cttcttacac aagattcgtg ctcttcaata atctctaaat ggtttttagt
     6721 cataatcgtc aaatccctcc gtctttttta ttcatacttg tttagccaga tcatttactt
     6781 ggaaaaaggt ccaatacgat acgtatattc agctttttgg ccttcttcag gaaatatctc
     6841 ttccggaaag cattctttca cttcataagc tgtatcgtga tctcgtgcca gtcgtttaaa
     6901 taaagactga gaggctttgt tctcttcagt gattgtcgcc tcaacgtaac gaacattttt
     6961 acatgcttta cgctcaagta agtcatttaa catttttgag gcaacccctt ttccgcgttg
     7021 agatgaatcc acaccaacct gccagataaa aatcacgtct tggtgttcag gttgaataaa
     7081 acctgtgatg aaaccaacga cttcacctcg ttctttagca accacacatg tctcagagaa
     7141 gtactcggcc atcataatgt acttataagc ggagttcaaa tcgagagtgg tgtttttggc
     7201 taatgcccac atagcctgcc catcttcttt agttggctgc tctaacgaaa atgtttcaat
     7261 tactgccgtt gtgcttttgc tcatcgtgac attccacctt tgttttaaat tgtattgagt
     7321 tgttgttcca ctcaacacgt ttcatctcaa ccagtcaaca ttattaatga taggtagttc
     7381 gtgttaaatt atcaaacatg gttaatctcg atgagtacgt atatatgtgt gtctgtatag
     7441 cttagtgctc ctcagaccgt tcagcttatt tattgctctt gtattgttag aattacagca
     7501 gatttgaatg taggtttttt gtaaattgac gatattagaa gcatatagaa aagagacctc
     7561 gctgtttcca gcaagatctc ttctaaggcg tacatttaga gtatattaat tgtttttagc
     7621 agaaagtttc tttcttttct cttcacgttc acgcatactt ttatcaagaa tttttttacg
     7681 taatcggata gagtgagggg tgacttcaca atattcgtct tcgtttaaaa attctaacgc
     7741 ttcttctagc gttaaaagac gtggtttttt catggtaacc gtctgttctt tcgttgcaga
     7801 acgaatattc gtctgttgtt tcatttttgt aatgttaaca gtcaggtcat tatctcgatt
     7861 atgttcaccg acaatcatac cttcataaat ttcagtacca gcttcaacaa aaaggatacc
     7921 gcgatcttct acttggagca tgccataagg cgtcgcttta ccattttcca ttgaaactag
     7981 aacaccttga cgccgtccgc ccacatagcc ttgcgctaca ggctgatagc tgtcaaatgt
     8041 atgatttaga ataccataac cttttgtttg agccataaat tcagtcgtat aaccaatcaa
     8101 gcctcttgat ggcactaaga agtccattct tacttgaccg tcacctttat tcaccatatt
     8161 taatagttcg gctttacgtt ccccaagcga ttccattacc gcacctgtat attcttcagg
     8221 tacgtccact tgagcgtttt caaatggttc acatggctta ccatctacct cacgaataat
     8281 aacttcaggc ttggacactt gtagttcata gccttctcgt cgtaagtttt cgatgaggat
     8341 ggagaggtga agttcaccac gacctgatac ggtccaaatg tctggagagc ttgtattttc
     8401 cactctgaga cttacatccg tttcaacttg tgttaataac cgagcttcaa ttttacgact
     8461 agtgacatga tcaccttcac gacctgcaaa cggactgttg tttacaagga aagtcatttg
     8521 caaagtaggt tcatcaatac gtacgagttc cattgggtca ggattatcag aaggacagat
     8581 agtttctcca accataatat cctcaatacc agcaatggca attaaatcgc ctgctttagc
     8641 ttcatcaatt tcaagtcgtt ttaatccaag aaatccgaaa agttttgata ttctgaacgt
     8701 tttgattgaa ccatcacgtt tcacaaggga agcttgatca ccttttcgaa tggtccctct
     8761 aaatacacga ccaacaccaa ttcttcctag gtagtcatta taatcaagca tcgtcacctg
     8821 gaattgaaac ggttcttgtg agttatcaat aggtgcaggg actgtttcga tcaccgtgtt
     8881 aaagagtacg cgcatgttat cgtcttgttt tgtaggatca gaatcagcgc tagctgtgcc
     8941 gttaatagca gaagcataca cgacagggaa ctctaattgt tcttcatccg ctcctaattc
     9001 tatgaagagg tcaaggactt catccacaac ttcttctgga cgggccatcg gacggtcaat
     9061 cttgtttacg acaagaacag gtgttaattt ttgttccaac gcttttttaa ggacgaatcg
     9121 tgtttgcggc atacagcctt caaatgcatc tacgactagt aaaacgccgt ccaccatttt
     9181 taaaatccgt tctacttcgc caccgaagtc tgcgtggcca ggggtatcaa gaatattaac
     9241 gcgaacacct tcataatcaa cggctgtgtt tttcgctaaa atagtgatac ctcgttcctt
     9301 ttcaatatca ttattgtcca tggctcgttc gttcacttgt tcattttctc tgaaagttcc
     9361 tgattgtttt aacagttcat caacgagtgt tgtttttcca tggtctacgt gggcgataat
     9421 tgcaatattt cttatgtctg accgtatagt cattataaaa cgctcctgtc taacttaaaa
     9481 ttgttcaact atactataat atcataaaaa agcaaccagt tcattaaaat tgtatgttat
     9541 tatgtttatt ttactaattg aatcgatttc attaagatgt ttttacaaca ataacatgat
     9601 tatgcggatg ataaaaagtg actgacatat atgacgacga ctcctgtgag aggaatcgac
     9661 ctctgaaaac attctgactg agctttgcaa aggcagggtg agttgaggat gagccccacg
     9721 gaaagcgttc atcagtcatg caaatatcgt taggaaataa cgatacaacc acgatttatc
     9781 aaatcgattc aattacttat ttaaaattaa aactataaat cccaattaaa agtatatcca
     9841 gtgaatgacg cataaatatc cctgtgattg ttactatacg aaaaataaaa gaactgtata
     9901 cgaagaatag tttattactt tgaaaatgaa caactaattg caagtattat catacaaatt
     9961 tgtggtgtaa aattccaaag tgttttttaa atggggaata ggggaggaga agagattaat
    10021 gtctaactaa ataaaaaacc atccaaaata ataatggacg gtaaaaatga tggatggctt
    10081 tatttttatt ttgtttgtcc ttcaccatat acatggtatt tcgttgttgt taatgcttca
    10141 agtcccattg gtccccgtac atgcagcttt tgtgtgctaa ttcctatttc tgcaccaaaa
    10201 ccaaattcaa acccatctgt aaaccgtgta gaggcgttat gataaacagt agaagcatca
    10261 attttattga aaaagagagc cacattttct tttgtctcgg aaataatcgc ttctgagtgc
    10321 tttgtcccat aaatctcaat atgatcaagg gcttcatgaa tgttcggaac aattttaaca
    10381 gctaacgtta
//
