LOCUS       NZ_CP019724            10399 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Streptomyces pactum strain ACT12 chromosome, complete genome.
ACCESSION   NZ_CP019724
VERSION     NZ_CP019724.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces pactum
  ORGANISM  Streptomyces pactum
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Streptomyces pactum ACT12 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2017) College of Life Sciences, Northwest A&F
            Unversity, No.22 Xinong Road, YangLing, Shaanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2016
            Assembly Method                   :: SMRT v. 2.3.0
            Assembly Name :: version 2.3.0 of the PacBio Single Molecule Real
            Time
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002005225.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:58:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,315
            CDSs (total)                      :: 7,221
            Genes (coding)                    :: 6,992
            CDSs (with protein)               :: 6,992
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 84 of 229
            Pseudo Genes (incomplete)         :: 195 of 229
            Pseudo Genes (internal stop)      :: 15 of 229
            Pseudo Genes (multiple problems)  :: 62 of 229
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:20:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6551596
            Orig. end                         :: 6561995
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019724.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Northwest A&F Unversity.
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="29"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    3..18
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.56"
                     /tool="antismash"
     gene            805..1413
                     /locus_tag="B1H29_RS28100"
                     /old_locus_tag="B1H29_28130"
     CDS             805..1413
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="B1H29_RS28100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28130"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_055416275.1"
                     /transl_table=11
                     /translation="MDLALPELPFPLRVHGPEGNWSYEDGVLTGTAGPRQDRFVPPTGE
                     ALESASDAPRLLGAPDGDFQLIARVTVGFNGAFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDCNSFTVEGSSVWLRVSRAGRAFAFHASLDGERWTFVRLFTLGDEKE
                     TGAALVGFMAQAPTGQGCEVTYDHLEFRPAWPADLRDGG"
     gene            1451..1954
                     /locus_tag="B1H29_RS28105"
                     /old_locus_tag="B1H29_28135"
     CDS             1451..1954
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329308.1"
                     /locus_tag="B1H29_RS28105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28135"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_199832273.1"
                     /transl_table=11
                     /translation="MIDERVTGIRVIRTVLPAEAETVAALHRRARATYYPDGLPDDGFD
                     WVAVWRRAAERADAHVLCAVERGGLVGIASFRVPDDGPSETVKLFQFHVDPDHWRTGVG
                     TALHSACVDQWRADGRRAAVLDVHVDNLRAQAFYARRGWVPDPEYPPAEGDHHLFLRFR
                     VPAR"
     gene            2041..2754
                     /locus_tag="B1H29_RS28110"
                     /old_locus_tag="B1H29_28140"
     CDS             2041..2754
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976951.1"
                     /locus_tag="B1H29_RS28110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28140"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_055416274.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDGVEVVHRSFELDP
                     GRAKDDVQPVLTMLTAKYGMSEAQARAGEDNLGAQAAAEGLDYRTRDRDHGSTFDMHRL
                     LHLAKERGRQEALLDAFYRANFADERSVFNDDERLVELAVTAGLDAVAAREVVADATAY
                     ADEVRADEREAAQLGATGVPFFVLDRAYGVSGAQPAEVFDQALTQAWGERTPLKLIDDG
                     DAEACGPDGCAVPRN"
     gene            2853..3911
                     /locus_tag="B1H29_RS28115"
                     /old_locus_tag="B1H29_28145"
     CDS             2853..3911
                     /NRPS_PKS="Domain: Aminotran_5 (16-307). E-value: 2.8e-24.
                     Score: 77.6. Matches aSDomain:
                     nrpspksdomains_B1H29_RS28115_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329310.1"
                     /locus_tag="B1H29_RS28115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28145"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_055416273.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.6e-19, bitscore:
                     66.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESFENLVRAEFAPKTTYLNTAGTGLLPARTLAAMRDAAASAAAG
                     QPFDMFADVEACRSAFARLVGVPATRVAAGASVATFSGLVAASLPAGAEVLTAEADFSS
                     LVNPLHTRWDLKVRSVPLERIAESVGPDTALVAVSSVQSADGRIADLAALRDAARAHGA
                     RTLVDASQSNGWLPLAADDFDYVAAVAYKWVLCPRGVAFLVVPEDLGAAAGLTPLFAGW
                     ASGERPWESCYGPVEELAHSARRFDESPALLSYAGARHSLALIEELGVAAVHAHDTALA
                     DGFREGVRSLGHEPVPAPGSAIVSVPGLGHRQGELSGAGVQVSDRAGNLRAAFHVYNSA
                     ADVDRLLDVLAG"
     aSDomain        2901..3773
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B1H29_RS28115_Aminotran_5.1"
                     /evalue="2.80E-24"
                     /label="B1H29_RS28115_Aminotran_5.1"
                     /locus_tag="B1H29_RS28115"
                     /protein_end="307"
                     /protein_start="16"
                     /score="77.6"
                     /tool="antismash"
                     /translation="TYLNTAGTGLLPARTLAAMRDAAASAAAGQPFDMFADVEACRSAF
                     ARLVGVPATRVAAGASVATFSGLVAASLPAGAEVLTAEADFSSLVNPLHTRWDLKVRSV
                     PLERIAESVGPDTALVAVSSVQSADGRIADLAALRDAARAHGARTLVDASQSNGWLPLA
                     ADDFDYVAAVAYKWVLCPRGVAFLVVPEDLGAAAGLTPLFAGWASGERPWESCYGPVEE
                     LAHSARRFDESPALLSYAGARHSLALIEELGVAAVHAHDTALADGFREGVRSLGHEPVP
                     APGSAIVSVP"
     gene            complement(4095..4994)
                     /gene="thpD"
                     /locus_tag="B1H29_RS28120"
                     /old_locus_tag="B1H29_28150"
     CDS             complement(4095..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="B1H29_RS28120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28150"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_199832272.1"
                     /sec_met_domain="PhyH (E-value: 1.6e-40, bitscore: 138.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITTTNVTDLYPTRGATEVATPRQDPVVWGSPDTPGPVSAGDLQ
                     ALDRDGFLAIDQLIGPDEVDVYRRELERLTTDPAIRADARSIVEPQSKEIRSVFEVHKI
                     SEVFAKLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTISVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEA
                     LTKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFA
                     APIRRPDFIGARDFTPVK"
     misc_feature    complement(4985..5005)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.93"
                     /tool="antismash"
     gene            complement(5001..5399)
                     /locus_tag="B1H29_RS28125"
                     /old_locus_tag="B1H29_28155"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="B1H29_RS28125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28155"
                     /product="ectoine synthase"
                     /protein_id="WP_043374438.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.2e-54, bitscore:
                     180.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEIEGTDRHVKSASGTWESKRIVLAKEKVGFSVHETILY
                     AGTETSMWYANHIEAVVCTKGDAELTDRETGKTYHITPGTMYLLNGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            complement(5488..6759)
                     /gene="ectB"
                     /locus_tag="B1H29_RS28130"
                     /old_locus_tag="B1H29_28160"
     CDS             complement(5488..6759)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 1.1e-71.
                     Score: 233.6. Matches aSDomain:
                     nrpspksdomains_B1H29_RS28130_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="B1H29_RS28130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28160"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_055416272.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.7e-75, bitscore:
                     251.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRALGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNAVLKRALIDYLERDGVTHGLDMSTTAKRRFLETFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVART
                     EWLRALSELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTATATLEAYWADGSAMEKQTRARGEQVEQHM
                     IAITEENLADVKEYRGRGLVWGLEFHDKDRAGKVAGRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGMKTLARAVRETA"
     aSDomain        complement(5662..6672)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B1H29_RS28130_Aminotran_3.1"
                     /evalue="1.10E-71"
                     /label="B1H29_RS28130_Aminotran_3.1"
                     /locus_tag="B1H29_RS28130"
                     /protein_end="366"
                     /protein_start="29"
                     /score="233.6"
                     /tool="antismash"
                     /translation="DRALGSRMYDEDGHEYLDFFAGAGSLNYGHNNAVLKRALIDYLER
                     DGVTHGLDMSTTAKRRFLETFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFLWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARTEWLRALSELCERQDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTVSKSISGYGLPMALTLFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATATLEAYWADGSAMEKQTRARGEQVEQHMIAITEENLADVKEYRGRGLVWGLEFH"
     gene            complement(6893..7408)
                     /gene="ectA"
                     /locus_tag="B1H29_RS28135"
                     /old_locus_tag="B1H29_28165"
     CDS             complement(6893..7408)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121394.1"
                     /locus_tag="B1H29_RS28135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28165"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_055416271.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPRVADGAALWRIARDSKVLDLNSSYSYLLWCRD
                     FAATSAVARDERGEPVGFVTGYVRPDRPDTLLVWQVAVDQAYRGRKLAAALLDGLAART
                     VSECGLTTLETTISPDNTASQRLFTSFAERHGARLEREVLFDTDLFPDGPHEPEVLYRI
                     GPLPAAAH"
     misc_feature    complement(7408..7422)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.76"
                     /tool="antismash"
     misc_feature    complement(7433..7445)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    7521..7537
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 18.33"
                     /tool="antismash"
     misc_feature    complement(7564..7579)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7574..7589
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     gene            complement(7737..8864)
                     /locus_tag="B1H29_RS28140"
                     /old_locus_tag="B1H29_28170"
     CDS             complement(7737..8864)
                     /NRPS_PKS="Domain: Aminotran_5 (73-300). E-value: 2.7e-28.
                     Score: 90.9. Matches aSDomain:
                     nrpspksdomains_B1H29_RS28140_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976957.1"
                     /locus_tag="B1H29_RS28140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28170"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_055416270.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.3e-21, bitscore:
                     73.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAGRFAAIEDQVAGLLGTGQDVVIMQGEALLPLE
                     GAIRAAAGPGTVALNVVTGPYGQTFGDWLRDCGATVHDLSVPFHTAVSAEQVRQAFARH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPGAPRRSYLSLLDWKERWIDGGRKALPHAP
                     AQLEMLALQACVKRIEAEGPQRVMARHASAAAATRAGALALGVLEPYVYEARDAAPVAT
                     TLRTPPDVDASELVARALESDPALPLAAGGGALAGEMIRVNHYGPGATRGAVRGCLAAL
                     GAVLAEKGLKADPEAARRAVEDAWARPAGPGLSEA"
     aSDomain        complement(7965..8645)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B1H29_RS28140_Aminotran_5.1"
                     /evalue="2.70E-28"
                     /label="B1H29_RS28140_Aminotran_5.1"
                     /locus_tag="B1H29_RS28140"
                     /protein_end="300"
                     /protein_start="73"
                     /score="90.9"
                     /tool="antismash"
                     /translation="LRDCGATVHDLSVPFHTAVSAEQVRQAFARHPEIDFVSLVHAEAA
                     TGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVS
                     AVSVSERAWARMAANPGAPRRSYLSLLDWKERWIDGGRKALPHAPAQLEMLALQACVKR
                     IEAEGPQRVMARHASAAAATRAGALALGVLEPYVYEARDAAPVATTLRTPPDVDASELV
                     ARALE"
     gene            9034..9528
                     /locus_tag="B1H29_RS28145"
                     /old_locus_tag="B1H29_28175"
     CDS             9034..9528
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028017.1"
                     /locus_tag="B1H29_RS28145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_28175"
                     /product="hypothetical protein"
                     /protein_id="WP_055416269.1"
                     /transl_table=11
                     /translation="MFALLLLVLVAVVTAVVLGRRAGTYARTAPEDAAPAPARPGGGFR
                     VLAGCAGAAAAVLYAWGALCVAFAVMEAEDGGTGSAPLRPCRTSEHPELSARIVDYSAS
                     YLPLRFRCETADGDGYDSDNVPGYVNPGVAVLALTAAAGAVAAGYATESRARAAARKEA
                     R"
ORIGIN
        1 cggtgacggt ctggtcaagt ccctcaccga cgcggtcagc gacaccgacg ccggggtgct
       61 gctcgaccac cgcatgcggg acaacttcgt cgccggggtc gccgagcgcg cggcgctgcc
      121 cgacgtggac tccccggtga cgccgggctc gggcggcgag cacaccgtcg ccgccgggtt
      181 cctgaccgtg ccggccgggc ggctgacggc ggagggcgag cacgacctgc tcctcgagga
      241 gtgcttcggg ccggtcaccg tggtggcccg ctacgaggac gaggccgagg tgcggggcgt
      301 gctgtcccgg ctccccggca acctcacggc gaccgtgcag ctctccgccg acgaggcggc
      361 gggccggggg cgcggggcgg agatcctcgc cgagttgacg ccgttggccg gacgggtgct
      421 ggtgaacggc tggccgaccg gggtcgccgt ggccgcggcg cagcagcacg gggggcccta
      481 cccggccacc acgtcgacgt ccacgtcggt cggcggtacg gcgatcgagc ggtggctgcg
      541 gccggtcgcg taccagggga cgcctgaagc gctgctgccg ctcgagctgc gggacggcaa
      601 tccgctgggg cttcctcgcc ggttcaacgg ggttctggag cggtaaccca gtgggggcgc
      661 gggtcggtcg cggctgcggg ctgcgggctg cgggctgcgg gctgcgggct gcgggctgcg
      721 cgctgcgcgc tgcgggctgc gcgcggccgg tcgcgcggtt ccccgcgccc ctgggacgtc
      781 caccggtcct gagaggatca cctgatggat ctcgcactgc ccgaactgcc ctttcccctc
      841 cgcgtccacg gccccgaggg gaactggtcc tacgaggacg gcgtcctcac cggaaccgcc
      901 ggtccccggc aggaccggtt cgtgccgccc accggcgagg cgctggagtc cgcgtccgac
      961 gcgccccggc tgctgggcgc tccggacggg gacttccagt tgatcgcccg tgtcacggtc
     1021 ggcttcaacg gcgccttcga cgccggggtc ctctacgtcc acgtcggcga gcgggcctgg
     1081 gccaagctct gcctggagta ctccccggac gtgcccaccg tctgcacggt ggtcacccgg
     1141 ggacactccg acgactgcaa ctccttcacg gtggagggga gttccgtctg gctgcgggtc
     1201 agccgggccg gacgggcctt cgccttccac gcctccctcg acggcgagcg ctggaccttc
     1261 gtccggctgt tcaccctggg ggacgagaag gaaaccggcg cggcactggt cggcttcatg
     1321 gcccaggcgc cgacggggca ggggtgcgag gtgacgtacg accacctgga gttccggccg
     1381 gcctggccgg cggacctgcg cgacggcggc tgacgcaacc gggcggtccg gatcccgcgt
     1441 cctgtgttgc gtgatcgacg aacgtgtgac cggaatccgc gtgatccgta ccgtcctgcc
     1501 cgccgaggcg gagaccgtcg ccgccctgca ccggcgggcc agggcgacgt actacccgga
     1561 cgggctcccc gacgacggct tcgactgggt ggccgtctgg cgccgcgccg ccgagcgggc
     1621 cgacgcgcac gtgctgtgcg ccgtggagcg gggcggcctc gtcggcatcg cctccttccg
     1681 ggtgccggac gacggcccct cggagacggt caagctgttc cagttccacg tcgaccccga
     1741 ccactggcgc accggcgtcg gcaccgccct gcactcggcc tgcgtcgatc agtggcgggc
     1801 cgacggcagg cgggcggccg tcctggacgt gcacgtcgac aacctgcgcg cccaggcctt
     1861 ctacgcccgc cggggctggg tccccgaccc ggagtacccg cccgccgagg gcgaccacca
     1921 cctgttcctg cgcttccgcg tgccggcgcg atgacacggg aatgaggccc gccgcccgga
     1981 cgttcccgta cgaggcgaag agggccttcg cacccgtacg acctggagaa gagccgagac
     2041 atgcgcgtcg agatctggag cgacatcgcc tgcccgtggt gctacgtggg caaggcccgc
     2101 ttcgagaagg cgctggcggc cttcccgcac cgtgacggcg tcgaggtggt ccaccgctcg
     2161 ttcgagctgg accccgggcg ggccaaggac gacgtccagc ccgtgctgac gatgctcacc
     2221 gccaagtacg gcatgagcga ggcgcaggcg cgggccggtg aggacaacct cggtgcgcag
     2281 gccgccgccg agggactcga ctaccgcacc cgggaccgcg accacggcag caccttcgac
     2341 atgcaccggc tgctgcacct cgccaaggag cggggccggc aggaggcgct gctggacgcg
     2401 ttctaccggg ccaacttcgc cgacgagcgc tccgtcttca acgacgacga gcggctcgtg
     2461 gagctggccg tcaccgccgg tctggacgcg gtcgcggcgc gcgaggtggt cgccgacgcc
     2521 accgcctacg ccgacgaggt gcgcgccgac gagcgcgagg ccgcgcagct cggcgccacg
     2581 ggggtgccgt tcttcgtgct cgaccgggcg tacggcgtct cgggcgccca gcccgccgag
     2641 gtcttcgacc aggcgctgac gcaggcgtgg ggcgagcgca caccgctgaa gctgatcgac
     2701 gacggggacg ccgaggcgtg cggcccggac gggtgcgcgg tgccccggaa ctgagaatgc
     2761 gcaggtggga gcccgtccat aagcgttgct tggaggaatc gcgtagaaat acgcaatgga
     2821 cggggaggtc gcggggacgc acagtggtgc ccatggagag cttcgagaac ctcgtccgtg
     2881 ccgagttcgc cccgaagacc acgtacctga acaccgccgg caccggcctg ctgcccgccc
     2941 ggaccctcgc cgcgatgcgt gacgcggcgg cgtccgcggc cgcggggcag ccgttcgaca
     3001 tgttcgccga cgtcgaggcg tgccgctccg ccttcgcccg gctcgtgggg gtgcccgcca
     3061 ccagggtcgc ggccggcgcc tcggtcgcca cgttcagcgg gctcgtcgcc gcctcgctgc
     3121 ccgcgggcgc cgaagtcctc accgcggagg ccgacttcag ctccctggtg aaccccctgc
     3181 acacgcgttg ggacctgaag gtgcgcagcg tgccgctgga gcggatcgcc gagtcggtgg
     3241 gaccggacac cgcgctcgtc gcggtcagtt ccgtccagtc cgccgacggg cggatcgcgg
     3301 acctcgccgc gctgcgggac gccgcccggg cgcacggcgc gcggacgctg gtcgacgcct
     3361 cccagtcgaa cgggtggctg ccgctggccg cggacgactt cgactacgtg gccgccgtcg
     3421 cctacaagtg ggtgctctgt ccccgcggcg tcgccttcct cgtcgtaccc gaggacttgg
     3481 gagcggctgc cggactgacc ccgctgttcg cggggtgggc gtcgggtgag cgtccctggg
     3541 agagctgcta cggccccgtc gaggaactcg cgcactccgc acggcgtttc gacgagagcc
     3601 cggctctcct gtcctacgcg ggtgcccggc actcgctggc cctgatcgag gagctcggcg
     3661 tggccgcggt gcacgcccac gacaccgcgc tcgccgacgg gttccgggag ggagtgcgct
     3721 cactgggcca cgagccggtg cccgcgccgg gctccgcgat cgtgtccgtg cccggactcg
     3781 gccaccggca gggcgagctg agcggggccg gcgtgcaggt ctccgaccgg gcgggcaatc
     3841 tgcgggccgc gttccacgtc tacaactccg cggcggacgt cgaccggctg ctggacgtgc
     3901 tggcgggctg agacggttcc ggcgcccttc cggcgccctt cggtccggct tccttccgcg
     3961 cgcttccggt gcccggaaag cgccgcaggg gcgtcgggga agcgccggaa gggcgccgtg
     4021 aatgggccgg gacagtatcg ggcaggggcc tcccgtccca cacgaaaagg cccctgcccg
     4081 agatcagatg cgggtcactt caccggtgtg aagtcccgcg ccccgatgaa gtccggccgc
     4141 cggatcggtg ccgcgaacgg ctcgaccgcc gtgttctcca cgctgttgaa cacgatgaac
     4201 acgttgctgc gcgggaacgg cgtgatgttg tcgccggaac cgtgcatgca gttgcagtcg
     4261 aaccaggtcg ccgagccggc cttgccggtg aacagcttga tgccgtactc cgaggccatc
     4321 ttcgtcagcg cctcgtcgga cggcgtcccc gcgtcctgca tctgcaggga cttcttgtag
     4381 ttgtccttcg gcgtggcccc cgcgcacccg aggaacgtct tgtgcgaccc cggcatgatc
     4441 atgagaccgc cgttggtgtc gtagttctcg gtgagcgcga tcgaaaccga gatcgtccgc
     4501 atgttcggca ggccgtcctc ggcgtgccag gtctcgaagt ccgagtgcca gtagaagccg
     4561 ctggccccga agcccggctt gacgttgatc cgggactggt ggacgtagac gtccgagccg
     4621 aggatctgcc gggcgcgccc gaccacgcgc tcgtcccgca ccagcttggc gaagacctcg
     4681 ctgatcttgt gcacctcgaa gaccgaccgg atctccttgg actgcggctc cacgatcgag
     4741 cgcgcgtccg cgcggatggc cgggtcggtg gtgagccgct ccagctcgcg ccggtagacg
     4801 tcgacctcgt ccggtccgat gagctggtcg atggcgagga agccgtcgcg gtccagcgcc
     4861 tgcaggtcac cggccgagac gggaccgggc gtgtccgggg agccccagac gaccgggtcc
     4921 tgccggggag tcgccacctc ggtggcgccg cgggtgggat agagatcggt gacgttcgtg
     4981 gtcgtgatgg tcacggtgac tcacacctcc tcggtgagca gcgggtagac gccgttctcg
     5041 tcgtgatcct cccgtccggt cacgggcggg ttgaagacac agatgcagtg gaagtcctcc
     5101 ttgaccttga gcgtgtgccg ctcgtggccg ttgaggaggt acatcgtgcc cggggtgatg
     5161 tggtacgtct tcccggtctc gcggtcggtc aactcggcgt cgcccttggt gcagacgacg
     5221 gcctcgatgt ggttcgcgta ccacatggac gtttccgtac ccgcgtacag aatcgtctcg
     5281 tgcacggaga agccgacctt ctccttcgcg aggacgatgc gcttgctctc ccaggtgccg
     5341 gacgccgact tcacgtgccg gtcggtgcct tcgatctcct tgaacgaacg gacgatcacg
     5401 gtgttgcgat gcctcctagg tgaagggtgt gcaagggtcc ccggcgcctt cggccgcatg
     5461 ggccgaaggc gccggaagta ggtgctgtca ggcggtctcg cgcacggccc gcgcgagggt
     5521 cttcatgccc tcgtccagct cgtcgggcgt gatggtgagc gccgggagca gcttgacgac
     5581 ctcgctctcg gggccggacg tctcgatgag cagcccgagt tcgaaggcgc gccccgcgac
     5641 cttgcccgca cggtccttgt cgtggaactc caggccccag accagaccgc ggccgcggta
     5701 ctccttgacg tcggcgaggt tctcctcggt gatggcgatc atgtgctgct cgacctgctc
     5761 accgcgggcg cgggtctgct tctccatcgc cgacccgtcg gcccagtacg cctcgagcgt
     5821 ggcggtcgcc gtgacgaagg cggggttgtt gccgcggaag gtgccgttgt gctcgcccgg
     5881 ctcccagacg tccagctccg gcttgaacag ggtcagcgcc atgggcaggc cgtagccgct
     5941 gatcgacttg gagacggtga cgatgtccgg cgtgatgccc gcctcctcga aggagaagaa
     6001 ggcgccggta cggccgcagc ccatctggat gtcgtcgacg atgagcagca tgtcctggcg
     6061 ttcgcacagc tcggacaggg cgcgcagcca ctcggtccgg gcgacgttga tgccgccctc
     6121 gccctgcacg gtctcgacga tcacggcggc cggcttgttc aggccggagc cctggtcctc
     6181 gagcagccgc tcgaaccaca ggaagtcctc gacggtgccg tcgaagtagt tgtcgaacgg
     6241 catgggcgtg ccgtgcacca gcgggatgcc ggcgccggcc cgcttgaagg cgttgccggt
     6301 cacggcgagc gagcccagcg acatgccgtg gaaggcgttg gtgaacgaga cgatcgactc
     6361 gcgccccttc accttccgcg ccagcttgag cgcggactcc acggcgttgg tgcccgtcgg
     6421 gcccgggaac atgaccttgt acggcaggtc acgcggccgg agcaccaggt tctggaacgt
     6481 ctccaggaag cggcgcttgg cggtggtcga catgtcgagc ccgtgcgtga cgccgtcccg
     6541 ctccaggtag tcgatcagtg cgcgtttcag tacggcgttg ttgtgcccgt agttgagcga
     6601 gcccgctccg gcgaagaagt cgaggtactc gtggccgtcc tcgtcgtaca tgcggctgcc
     6661 gagcgcacgg tcgaagacgg tgggccagcc gcggcagtag ctgcgcacct cggactcgac
     6721 tgtctcgaag acactgaggt cgggctgggt gatggtcacg acgaatcgct cctcgttggg
     6781 tgagggtggg agaggaaggt cgtacggatc cgggggaccg gtgcggtccc ggccgggaag
     6841 tctgaagggc cggactggca cggggtgccg ggccgtccgg gcgaccggtc ggtcagtggg
     6901 cggcggcggg cagtggcccg atgcggtaca ggacctcggg ctcgtggggc ccgtcgggga
     6961 agaggtccgt gtcgaacagc acttcgcgct ccagtcgggc gccgtgccgc tcggcgaacg
     7021 aggtgaacag tcgctgggag gcggtgttgt cgggcgagat cgtggtctcc agggtggtca
     7081 gcccgcactc cgacacggtc cgggcggcca gcccgtcgag cagggcggcg gccaacttcc
     7141 gtccacggta cgcctggtcc accgccacct gccacacgag cagggtgtcc ggccggtccg
     7201 gtcgtacgta accggtgacg aagccgaccg gctcaccgcg ctcgtcccgg gcgacggccg
     7261 acgtggcggc gaagtcccgg caccagagca gatagctgta ggacgagttc aggtcgagga
     7321 ccttcgaatc ccttgctatc cgccacaggg cggccccgtc ggccactctc ggacggtcga
     7381 tttgcaggtc tgcttgcgcg gcagtcatgc aaataaaatt tacccagcga aatttgaaat
     7441 tgcatcgccg gatggggtta cgtatgagcg cgtcatgtgt tatcacgcgg gcgcgtgccg
     7501 ccgcgcgagg tggcggcgat gtgtcataat ttgacctggt cataggccgc aaaacgggca
     7561 gcatgtggat cgggtcacaa tcgcgtaacc cgtgcgagac gtgcccgaaa tgcgaaggtc
     7621 gaggttcgtg aaatctttgt gtttaggtct cgggaaagcg ggcagaagaa tacgggaagc
     7681 tgtgctgagc gtaattactt aattctgttt gtgtgagctt cgggaattcc ctgaattcac
     7741 gcttccgaaa ggccgggtcc cgcgggccgg gcccaggcgt cctcgacggc ccgccgagcc
     7801 gcctccggat cggccttcag ccccttctcg gccagcacgg cacccagtgc cgccaggcag
     7861 ccccgcacgg ccccgcgcgt cgcgcccgga ccgtagtggt tgacgcggat catctccccg
     7921 gccagcgcgc ccccgcccgc ggccagcggc agcgccgggt cggactccag ggcccgggcg
     7981 accagctccg aggcgtcgac gtccggcggc gtccgcagcg tcgtcgcgac cggcgccgcg
     8041 tcccgggcct cgtacacgta gggctccagg acccccagcg cgagcgcgcc ggcccgggtg
     8101 gccgcggcgg ccgacgcgtg ccgcgccatc accctctgcg ggccctcggc ctcgatgcgc
     8161 ttcacgcacg cctgaagcgc gagcatctcc aactgcgccg gagcgtgcgg cagcgccttg
     8221 cgcccgccgt cgatccagcg ctccttccag tccagcagcg agaggtacga gcggcgcggc
     8281 gccccggggt tcgccgccat ccgcgcccac gcccgctcgc tcaccgacac cgccgacacc
     8341 ccggccggac cgcccatcgc cttctgcgcg ccgatgacgc acaggtccac cccccacgcg
     8401 tccggcagta ccggctcggc gcccaccgag gccacggcgt ccaggtagaa cagcgcgccg
     8461 tgcgcccgga ccacctcgcc gatctccgcg accgggttgg tgttcccggt cgccgcctcg
     8521 gcgtgcacca gggacacgaa gtcgatctcc gggtgccggg cgaaggcctg gcggacctgc
     8581 tcggcggaca cggccgtgtg gaacggcacg gagaggtcgt gcacggtcgc gccgcagtcc
     8641 cgcagccagt ccccgaacgt ctgcccgtac ggccccgtga cgacgttcag cgcgacggtg
     8701 cccggccccg cggcggcgcg gatcgcgccc tccagcggca gcagtgcctc gccctgcatg
     8761 atcacgacgt cctggccggt gccgagcagg cccgcgacct ggtcctcgat cgcggcgaaa
     8821 cggccggcgc tcagcggggc gaggtccagg aaggggtggg tcactgtggt gctctcttcg
     8881 ctcacgggca ggtgtacggc acgtacgaag tcgagagtaa ccacggcccc agccgccact
     8941 cgcgccggag ggcatccgtt gcgacacccc cacgcccgga attgtggctt ccgccacagc
     9001 ggtgatgatc gtcgggctgg tggactcgga cccgtgttcg ctcttctgct gctcgtcctc
     9061 gtggcggtcg tgaccgccgt cgtcctcggc cgccgcgccg ggacgtacgc ccgcaccgcc
     9121 cccgaggacg ccgctcccgc gcccgcccgc cccggtgggg ggttccgcgt actggcgggg
     9181 tgcgccggtg cggccgccgc cgtgctgtac gcctggggcg cgctgtgtgt ggcgttcgcg
     9241 gtcatggagg cggaggacgg cggcacggga tcggcgccgt tgcggccctg ccgcacgagt
     9301 gagcacccgg agctgtccgc ccggatcgtc gactactcgg cctcctacct gcccctccgc
     9361 ttccgctgcg agaccgccga cggcgacgga tacgactccg acaacgtccc cggctacgtc
     9421 aaccccggcg tcgccgtcct cgccctgacc gccgcggcgg gcgccgtcgc ggccgggtac
     9481 gcgaccgagt cccgcgcccg cgcggcagca cggaaggagg cccggtagac cgggccttcg
     9541 aaccctcccg gtgcccggtc taaggtgcgg gcatgagcga tcgcacggtg ctgcacgtga
     9601 agggtcgggt gctcgtcggg ccgcgggacg aggacgtccg cgacgagctg tgggtgatcg
     9661 acggccggat ctcgtacgac cgccccgccg gggccgccga cgtccgcacc gtcgagggct
     9721 gggccctgcc cggcctggtc gacgcgcact gccacgtcgg cctcgaccag cacggggccg
     9781 tccccgagga cgtcgccgag aagcaggcgc tgaccgaccg cgaggcgggc accctcctca
     9841 tccgcgacgc gggctccccc tccgacaccc gctggatcga cgaccgcgag gacctcccga
     9901 agatcatccg ggccggccgg cacatcgccc gcacccggcg ctacatccgc aactacgcct
     9961 gggaggtcga gcccgaggac ctcgtcgcct acgtcgccca ggaggcccgg cgcggggacg
    10021 ggtgggtgaa gctggtcggc gactggatcg accgcgacct cggcgacctg tccgcgtgct
    10081 ggccgcgcgg cgccgtcgag gcggcgatcg cggaggccca ccggctgggc gcccgcgtca
    10141 ccgcgcactg cttcgccgag gactccctgc gcgacctggt cgaggccggc atcgactgcg
    10201 tcgaacacgc cacgggcctg acggacgaca ccatcccgct cttcgccgag cggggcgtcg
    10261 ccatcgtgcc caccctcgtc aacatcgcca ccttcccggc gctcgccgac ggcggcgagt
    10321 ccaggttccc gcgctggtcg gcccatatgc gccggctcca cgaacgccgc tacgacaccg
    10381 tgcgcgacgc ctacgacgc
//
