LOCUS       NZ_CP019724            10507 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Streptomyces pactum strain ACT12 chromosome, complete genome.
ACCESSION   NZ_CP019724
VERSION     NZ_CP019724.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces pactum
  ORGANISM  Streptomyces pactum
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Streptomyces pactum ACT12 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2017) College of Life Sciences, Northwest A&F
            Unversity, No.22 Xinong Road, YangLing, Shaanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2016
            Assembly Method                   :: SMRT v. 2.3.0
            Assembly Name :: version 2.3.0 of the PacBio Single Molecule Real
            Time
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002005225.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:58:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,315
            CDSs (total)                      :: 7,221
            Genes (coding)                    :: 6,992
            CDSs (with protein)               :: 6,992
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 84 of 229
            Pseudo Genes (incomplete)         :: 195 of 229
            Pseudo Genes (internal stop)      :: 15 of 229
            Pseudo Genes (multiple problems)  :: 62 of 229
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:20:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5599481
            Orig. end                         :: 5609988
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019724.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Northwest A&F Unversity.
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10507
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="27"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10507
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10507
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5507](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5507
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(784..2049)
                     /locus_tag="B1H29_RS23890"
                     /old_locus_tag="B1H29_23945"
     CDS             complement(784..2049)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527564.1"
                     /locus_tag="B1H29_RS23890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_23945"
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_079160832.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.4e-31,
                     bitscore: 108.2, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALSPPGRRPRVLHITQPVDGGVARVVTDLVREQRTHGLDVAVAC
                     PDSALTTRLRALGAQVRHWQATRSPGPSLVREVRQLARLIDQVRPDVVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEAAGEGTAALALVWERWAARWATRVVCVSKAERETGVHAG
                     ISGRWTVVPNGIDPEHYHPAAAGTVRAGLAPLAGVDPAVPLAVCVGRLCRQKGQDVLLR
                     AWDAVVRRVPGARLVLVGDGPDLDRLRRVAPGSVLFTGAVTEPSPWYQAADLVVLPSRW
                     EGMALAPLEAMACGRPVVVTDVDGARESLPPSLTGRCLVRPEDPAELAEAVAGLLLDPP
                     LREFLGDRGRRHVLSLHDVRHTAEQIADVYRDLLGTWSAARGACPSRGAEHGQPLGTGC
                     GTEANRTTQHVESTQNRESIHS"
     gene            complement(2094..3182)
                     /locus_tag="B1H29_RS23895"
                     /old_locus_tag="B1H29_23950"
     CDS             complement(2094..3182)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056483.1"
                     /locus_tag="B1H29_RS23895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_23950"
                     /product="hypothetical protein"
                     /protein_id="WP_055417733.1"
                     /transl_table=11
                     /translation="MRHSLGQRLRSATVGVLALTTMWAPAGSAAAAPPAPEAADLAFAQ
                     RYAALQHGDIVRAANTAISCRTDQARSCPDVQAGGAGVNGGFDMFYVDGDRDPNTYNSS
                     RAEVRLPAGSRVTYARLYWGGNLRVGEQKPPEDNGRVLIAEPGGEYKELLADTVVGHRV
                     TDSADAFQASADVTRLVRDSGPGHYTVAQVNVAAGRSAAGAWGGWTLVVAYENPAEPLR
                     HLALWDGFAPLRSRAAELRMRGLRFPAGAGGSVGLVAYDGDRGTSGDSLTVTTGGPAAD
                     SFRLSDPTNPPGDILNSTISGPGESSPARVPAYANTLGYDSDVLDLAGRLPHAGDEATF
                     RLSSERDAAWAGVLFAAVDARR"
     gene            3181..3312
                     /locus_tag="B1H29_RS39880"
     CDS             3181..3312
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B1H29_RS39880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_279636559.1"
                     /transl_table=11
                     /translation="MTVDALSEQVVAGSGTSTMHPPVVRQEVRNSSHQVGEFTEIQA"
     gene            3558..4232
                     /locus_tag="B1H29_RS38215"
                     /old_locus_tag="B1H29_23955"
     CDS             3558..4232
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826199.1"
                     /locus_tag="B1H29_RS38215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_23955"
                     /product="hypothetical protein"
                     /protein_id="WP_055416984.1"
                     /transl_table=11
                     /translation="MPTLSARRIALGALCAALLAGTTGPAAMAADSTSDRVRVVPADAP
                     LTGADALLTQVRNLEARGVELGAVADLLTAVIRTDDGQLPPDEAEELGEAAKEALEEAA
                     AETQAATATATATATPTPATEPLPVTGAELTSDALDAVREAVDRLLESLLPGNAADQVL
                     FSVDDLLTEVGRLVDALTPGDPQASTLPAPATLPGISLPVPLVPGVTLPAISPLGSVLL
                     PS"
     misc_feature    complement(3559..3575)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.92"
                     /tool="antismash"
     gene            4492..4776
                     /locus_tag="B1H29_RS23905"
                     /old_locus_tag="B1H29_23960"
     CDS             4492..4776
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003504.1"
                     /locus_tag="B1H29_RS23905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_23960"
                     /product="hypothetical protein"
                     /protein_id="WP_055416983.1"
                     /transl_table=11
                     /translation="MKPLKVAAVIVGSVIASGAAAPAFAHNAGDLAPTSLNNGTATPFK
                     NVSVLDIPLDDPMNVIDNRNGDSPLYTVADAKNALNGNESLQRGLSLQG"
     gene            5001..5507
                     /locus_tag="B1H29_RS23910"
                     /old_locus_tag="B1H29_23965"
     CDS             5001..5507
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028525.1"
                     /locus_tag="B1H29_RS23910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_23965"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_055416982.1"
                     /sec_met_domain="melC (E-value: 7.4e-50, bitscore: 167.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVISSGDVPVGAEEAAPAPALPGPEGRTRRDVARGLRACALVAAL
                     TPVVAASRPPRSRSVPTDAPGGPDFDQTYRGRRVRGVWIPARGAAGGGRWHVTVDGRPL
                     HLMRRADGTWLSMVDHYCSYPTALDTARAAVDALGPGQRLRAAPASAPLGQGHPHEGGR
                     HGVRA"
     misc_feature    5007..5021
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            5491..6357
                     /locus_tag="B1H29_RS23915"
                     /old_locus_tag="B1H29_23970"
     CDS             5491..6357
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="B1H29_RS23915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_23970"
                     /product="tyrosinase family protein"
                     /protein_id="WP_055416981.1"
                     /transl_table=11
                     /translation="MAYVRKDAGTLTGAERRRFVKALLELKRRGEYDEFVRMHVDHYSS
                     DGERGLRTAHLAPSFLPWHRRFLLDLENALRRVDSSVTVPYWDWTRDRTTTSAPWTADL
                     LGGNGRRSDLRVTTGPFAHREGNWTIRVGVTEGEFLTRDLGRRRAPIDLPTKSDLEWAL
                     KDTAYDVSPWDSTVTRGFRNKLEGWGTGRGSASWRNHNRVHRWVGGEMLTATSVNDPVF
                     WLHHAFVDLQWYRWQRRHRGAAYLPATPPGRGDDQHGRVVARHEKLPPWGVTPGELENV
                     DRIYRYA"
     misc_feature    6472..6485
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.77"
                     /tool="antismash"
     misc_feature    6581..6601
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.91"
                     /tool="antismash"
     gene            6624..6905
                     /locus_tag="B1H29_RS23920"
                     /old_locus_tag="B1H29_23975"
     CDS             6624..6905
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990345.1"
                     /locus_tag="B1H29_RS23920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_23975"
                     /product="hypothetical protein"
                     /protein_id="WP_063787481.1"
                     /transl_table=11
                     /translation="MRKIQKAAVVVAMLGSVSALGAGTAMAGEADTSFDVKGGGCSVSG
                     DSNQLIGNVGVLNGVVGGILTFGNDGDHGTQGNEQGHKVDCSTRGFTG"
     misc_feature    6961..6975
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.75"
                     /tool="antismash"
     gene            7080..7334
                     /locus_tag="B1H29_RS23925"
                     /old_locus_tag="B1H29_23980"
     CDS             7080..7334
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B1H29_RS23925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B1H29_23980"
                     /product="hypothetical protein"
                     /protein_id="WP_055416980.1"
                     /transl_table=11
                     /translation="MQNLKKIAVVAGFLGGSVLMGLGTAQAQSFDIPVCDEKASDGHCG
                     QKREYQYTTENGQHVHVVVAREGCAIKGNSTVDCSIHHP"
     misc_feature    7086..7100
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.49"
                     /tool="antismash"
     gene            complement(7891..8862)
                     /locus_tag="B1H29_RS23930"
                     /old_locus_tag="B1H29_23985"
     CDS             complement(7891..8862)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (51-280). E-value: 3e-21.
                     Score: 68.1. Matches aSDomain:
                     nrpspksdomains_B1H29_RS23930_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987903.1"
                     /locus_tag="B1H29_RS23930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_23985"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_055416979.1"
                     /sec_met_domain="PF00561 (E-value: 5.7e-16, bitscore: 57.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 5.1e-10, bitscore:
                     39.1, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAVTDTQELTAERSRPVAAPPERVEERGLVVDGFAYDCRIVHQ
                     RAPRTEPLLLLGGSSQNKYSWQGHEKYLAPLCTLITMDLPGYGTADFLPARYGIDFLAS
                     AVKHMLVEIKMPRVNLFGGCFGEVVGLRFAQLYPEFVSRIIFCGAAKRLPEIYTAAVPR
                     LSHALERGRIEEAADGLVRLFMSNPSAGRVRKHAAVSRLLQRQFLDQTPDEITKAVEHN
                     TRLLTHGCYQPLPVPDVPSLVFTGEYDTLCTPQMGRELAAAMPAARFTTIKEADHLVTV
                     ERRQESADLIGRFCTDDPIDDLPYCTPMESPRTGPVAAAGCR"
     aSDomain        complement(8023..8709)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B1H29_RS23930_Abhydrolase_1.1"
                     /evalue="3.00E-21"
                     /label="B1H29_RS23930_Abhydrolase_1.1"
                     /locus_tag="B1H29_RS23930"
                     /protein_end="280"
                     /protein_start="51"
                     /score="68.1"
                     /tool="antismash"
                     /translation="PLLLLGGSSQNKYSWQGHEKYLAPLCTLITMDLPGYGTADFLPAR
                     YGIDFLASAVKHMLVEIKMPRVNLFGGCFGEVVGLRFAQLYPEFVSRIIFCGAAKRLPE
                     IYTAAVPRLSHALERGRIEEAADGLVRLFMSNPSAGRVRKHAAVSRLLQRQFLDQTPDE
                     ITKAVEHNTRLLTHGCYQPLPVPDVPSLVFTGEYDTLCTPQMGRELAAAMPAARFTTIK
                     EADHLVT"
     tRNA            complement(9216..9288)
                     /anticodon=(pos:complement(5608734..5608736),aa:Arg,
                     seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="B1H29_RS23935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="B1H29_23990"
                     /product="tRNA-Arg"
     gene            complement(9216..9288)
                     /locus_tag="B1H29_RS23935"
                     /old_locus_tag="B1H29_23990"
     misc_feature    9580..9594
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.96"
                     /tool="antismash"
     misc_feature    9583..9599
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.58"
                     /tool="antismash"
ORIGIN
        1 acgacgggga gtcggcgtcg atctcccaga tcacgcagcc cgactcggcc agcgtgcgca
       61 gcagcggccc ccgttcggcg cgtaccgagg ggtgaacggc cagcaccgcc cgcaccccgt
      121 tctggatgac cgcccgctcg atctcctcga ccgtccgcag caccgggatc ctctcggtgc
      181 cgtccggctg ctcggcgacg atccccaccg ggcgcacgcc gcacggtgga tggcgcagca
      241 cggcgtcggc gacgcgctgc gcggtcgcgg ccgggccgat gacgagtgcg gcgcgcgggt
      301 ggcgcagcag tgcggcgcgc cgccgtccgt gcacggccgc ccgcagcaca cagccggccg
      361 tcgcctgcag ggcgaggccc gtcagcacgg tgtcggcgga cagtgcgtgc tcgggccggt
      421 acgccgccca gagcgcggcg gacgccagcc aggcgacggc gatgcgcccg cacacggcgg
      481 gcagttcctc cagcagcccc ggcaccggcg gccgcctgtc ccgcggacgc agcagcagcg
      541 atgcggcgac aagcagggcg atgagcagcg ggtggcgctg ggcgtcggtg agcgccagcg
      601 tgcccagcag cgcggcgacg gcgtccgcgg cgatcagcgg gaggggcgaa gccggccggg
      661 ccggggaccg cggggcgggg gaccgggaac cggtggcggt gccgagcggc ggcatgacgg
      721 agacggacga gagttcgtgg tcccgtggcg ttacgcccag ggagggcacg gtacgttcgg
      781 cagtcacgag tggatggact ccctgttctg ggtggactcg acgtgctgtg ttgtccggtt
      841 cgcctcggtg ccacagcccg tgccgagcgg ctgaccatgc tcggcgccgc gggacgggca
      901 cgcccctcgt gcggcggacc aggtgccgag caggtcgcgg tagacgtccg cgatctgctc
      961 ggcggtgtgc cgtacgtcgt ggagggacag cacgtgccgg cggccccggt cgccgaggaa
     1021 ctcgcgcagc ggcgggtcga gcagcagtcc ggcgacggcc tcggccagct ccgccgggtc
     1081 ctccggccgg accaggcacc gcccggtgag ggaaggcggc aggctctcgc gggcgccgtc
     1141 gacgtccgtg accaccacgg gccgtccgca ggccatcgcc tccagcgggg ccagcgccat
     1201 gccctcccag cgagacggca ggacgacgag gtcggcggcc tggtaccagg gggagggttc
     1261 ggtgacggct cccgtgaaca gcacggaccc gggggcgacc cggcgcagcc ggtcgaggtc
     1321 cggtccgtcg ccgaccagga cgaggcgggc gccgggcacc ctgcgcacta cggcgtccca
     1381 cgcccgcagc aggacgtcct gccccttctg ccggcacagc cgtcccacac agaccgccag
     1441 cggcaccgcc gggtcgacgc cggccaacgg agcgagcccg gcccgtacgg tgccggcggc
     1501 ggccgggtgg tagtgctcgg gatcgatgcc gttggggacg acggtccacc ggccggagat
     1561 cccggcgtgc acgccggtct cgcgttcggc cttgctcacg cacaccaccc gcgtggccca
     1621 gcgggccgcc caccgctccc acacgagcgc gagggcggcg gtgccctcgc cggccgcctc
     1681 gaacgaccag gcgtgcggct ggaagaccgt cggaatccgc ccgcgcactg cgagccgccc
     1741 ggcgagcccg gccttcgcgc tgtgcgcgtg caccacgtcg gggcgcacct ggtcgatcag
     1801 gcgcgcgagc tgccgtacct cccgcacgag cgaaggaccc ggcgaccgcg tcgcctgcca
     1861 gtgccgtacc tgcgcgccga gcgcccgcag ccgggtggtg agcgcgctgt cggggcaggc
     1921 cacggcgacg tccaggccgt gcgtccgctg ttctcggacc aggtccgtca cgacgcgcgc
     1981 gaccccgccg tccaccggtt gggtgatgtg caggacccgc ggccgacggc cggggggcga
     2041 cagggccacg gattcctcgc tcgggtaggc gccggggacg gggtgcggcg tgttcaccgc
     2101 cgggcgtcga ccgcggcgaa gagcacaccg gcccacgccg cgtcccgctc ggagctgagc
     2161 cggaaggtcg cctcgtcacc ggcgtgcggc aggcgtcctg cgagatcgag cacgtcggag
     2221 tcgtagccga gggtgttggc gtacgccggc acacgggccg gggaggactc cccgggcccg
     2281 ctgatggtgg agttgaggat gtcgcccggg gggttggtgg gatcggagag gcggaaggag
     2341 tcggctgcgg gcccgccggt ggtcacggtg agcgagtcgc cgctggtgcc gcggtcgccg
     2401 tcgtacgcca ccaggcccac gctgcccccg gcgcccgcgg ggaagcgcag gccgcgcatc
     2461 cggagttccg ccgcgcggga ccgcaggggc gcgaagccgt cccagagcgc gaggtgccgc
     2521 aggggttccg ccgggttctc gtacgccacc accagcgtcc agccgcccca tgccccggcc
     2581 gccgacctgc cggcggccac gttgacctgg gccacggtgt agtggcccgg cccgctgtcc
     2641 cgcaccagcc gcgtcacgtc cgccgaggcc tggaaggcgt ccgcgctgtc ggtcacgcgg
     2701 tgtccgacga ccgtgtccgc gaggagttcc ttgtactccc cgccgggttc ggcgatcagc
     2761 acccgcccgt tgtcctccgg cggcttctgc tcgcccacgc gcaggttgcc gccccagtac
     2821 aggcgtgcgt acgtcacccg cgagccggcg ggcaggcgga cctccgcgcg ggaggagttg
     2881 taggtgttcg ggtcgcggtc gccgtccacg tagaacatgt cgaagccgcc gttcacaccg
     2941 gcgccgcccg cctgcacgtc cgggcacgaa cgtgcctggt ccgtgcggca actgatggcg
     3001 gtgttggccg cgcgcacgat gtcgccgtgc tggagcgcgg cgtaccgctg ggcgaacgcg
     3061 aggtcggccg cctcgggtgc gggcggtgcc gccgccgcgg aaccggcggg tgcccacatc
     3121 gtggtgaggg cgaggacgcc caccgttgca ctgcgaagcc gctgacccag ggaatggcgc
     3181 atgaccgtcg atgccctttc ggagcaggtg gtggcgggca gcggcacgag cacgatgcat
     3241 ccgcctgtag tgcgtcagga ggttcgcaac tctagccatc aagtgggaga attcaccgaa
     3301 attcaggcat aaggaaaact gtgttgggaa tgtgaaggcc cctgcggtcg gtcgtcccgc
     3361 cctgaggacc ggcccagtgc ggcacctgcc gttccgccgg acggggaggg gtgcggccgg
     3421 cccggcgtag cgcctgatca ggcatgtcgg ggcagcaggg tgaagggcgg gacggcgaac
     3481 gacgcacggc cggttgcggg gctccatggg cgcgttcacg gtgagctcaa gctccgagct
     3541 ccgtcgaaag gaaccccatg cccactctgt ctgcacggcg catcgcactc ggcgcactgt
     3601 gcgccgccct cctggccggg accaccggcc ccgccgccat ggcggccgac tcgacctccg
     3661 accgcgtccg cgtggtgccg gccgacgccc cgctcaccgg cgccgacgcg cttctcacgc
     3721 aggtcagaaa cctcgaggcc cgcggggtcg agctcggagc ggtcgccgac ctgctgaccg
     3781 ccgtgatcag gacggacgac ggccaactgc ccccggacga ggccgaggag ctgggtgagg
     3841 ccgcgaagga ggccttggaa gaggcggcgg cagagactca ggcggccacg gccacggcca
     3901 cggccacggc cacgccgacc ccggcgacgg agccgctgcc cgtcaccggg gccgagctca
     3961 cgagcgacgc gctggacgcc gtgcgggagg cggtcgaccg cctcctggag tcgctcctgc
     4021 ccggcaacgc cgccgaccag gtgcttttct cggtcgacga cctgctgacc gaagtgggca
     4081 ggctcgtcga cgcgctcacc cccggcgacc cgcaggcgtc gaccttgccc gctccggcga
     4141 cgctgccggg catctcgctc ccggtgccct tggtgccggg cgtcaccctt cccgcgattt
     4201 cgccgctggg atcggtgctc ctgccgtcgt agcgagcgcc gcacgagtgt gagtacggcg
     4261 ccgggccgtt tcgtcgtggc gggtcttcgg gggcgattcc acgaccgacg ttctttccgg
     4321 ccttctccgc tccttctcgc gggcgtcccc ctctcggggg ccggtctttc atagttctca
     4381 tatgagaact cggcaatctc cggtcctccc ggttccgttc cgccgctggg ctcgttggac
     4441 aggcgacaga aaaagcctcc ggcgacctgc gttgccgaag aaaggaacac gatgaagccc
     4501 ctgaaagtcg ccgccgtcat cgtcgggtcc gtgatcgcct ctggtgccgc cgcgcccgcg
     4561 ttcgcccaca acgccggtga cctggcgccc accagcctca acaacggcac ggcgaccccg
     4621 ttcaagaacg tgtccgtcct cgacatcccg ctggacgacc cgatgaacgt gatcgacaac
     4681 cggaacggcg actcgccgct ctacacggtg gcggacgcca agaacgcgct gaacggcaac
     4741 gagtcgctgc agcgcggtct gtcgctgcag ggctgagacc acgccttcga aggacggccc
     4801 tcgacggagt ccgagggccg tcctttcgtg tgcacggccg gtgactccgg ctcgcttccc
     4861 gtgccgggcc ggtgagccgg gggacgcgtc cgtatccccg aaccggtcgc ggatggtcac
     4921 cggtgcggcc gcacggcggt gaccgcgagg gacgttcggc ggttacggct gcgaagagcc
     4981 gaacggacga ggagtgcacc atggtcatca gcagtggcga cgtaccggtg ggggcggagg
     5041 aggcggcacc ggccccggcg ctgcccggac cggagggcag aacacggcgg gacgtggcgc
     5101 gcggtctgcg cgcctgtgcg cttgtcgccg ccctgactcc ggtggtcgcc gcatcccggc
     5161 cgccacgctc ccggtccgtg cccacggacg ctcccggcgg tcccgacttc gaccagacct
     5221 accgcggccg ccgcgtccga ggtgtgtgga tccccgcgcg cggcgcagcc ggtggcggcc
     5281 ggtggcacgt cactgtcgac gggcgccccc tgcacctgat gcgccgcgcc gacggcacct
     5341 ggctgagcat ggtcgaccac tactgctcgt acccgacagc gctcgacacc gcccgcgccg
     5401 ccgtcgacgc gctcggcccg gggcagcggc tgcgggccgc cccggcttcg gcgccgctgg
     5461 gccaggggca cccgcacgag gggggccggc atggcgtacg tgcgtaagga cgccggcacg
     5521 ctcacggggg ccgagaggcg cagattcgtc aaggcgctgc tggagctcaa acggcgcggc
     5581 gagtacgacg agttcgtgcg gatgcacgtc gaccactact cctccgacgg cgagcgcgga
     5641 ctgcgcaccg cgcacctggc gccgtccttc ctgccttggc accgccggtt cctgctcgac
     5701 ctggagaacg ctctgcgccg ggtggattcc tcggtgaccg tcccgtactg ggactggacc
     5761 cgcgatcgca cgacgacctc cgccccgtgg acggcggatc tcctcggcgg caacgggcgc
     5821 cgctccgacc tccgggtgac gaccggtccg ttcgcccacc gggagggcaa ctggaccatc
     5881 agggtgggcg tgaccgaggg cgagttcctc acccgggacc tcggcaggcg acgcgccccg
     5941 atcgacctgc ccacgaagag cgatctggag tgggcgctga aggacaccgc ctacgacgtg
     6001 tcgccctggg actcgacggt cacccggggc ttccgcaaca aactcgaggg ctggggcacc
     6061 ggccggggca gcgcctcatg gcgcaaccac aaccgggtgc accgctgggt cggcggagag
     6121 atgctcacag ccacctccgt caacgacccc gtcttctggc tgcaccacgc tttcgtcgac
     6181 ctgcagtggt accgctggca gcgccggcac cgcggcgccg cctaccttcc cgcgacaccg
     6241 ccgggccgcg gcgacgatca gcacggccgg gtcgtggcac ggcacgagaa gctgccgccg
     6301 tggggcgtga caccgggcga gctggagaac gtcgaccgga tctaccggta cgcatgagat
     6361 gaaaacgcat gagacgacgc ggtacggggg agccccggcg ccggagggcg cgccagggct
     6421 ccgggtgctg tccccgcgcc gggaccccga cacgccggtc tcggccgtcc gatttctaca
     6481 caagagctac acaacgcctg atgcgaagaa aatttacgaa gtcgggtgtt tcgtcgcgcg
     6541 aagtcaggag atgtcgtcta gagtgaacac cgtaagtgac cagcccagca cgggctgtgt
     6601 tgcccagagg aaacggagag gtaatgcgca agatccagaa ggctgccgtc gtcgtcgcga
     6661 tgctcggcag tgtcagcgcc ctcggcgccg gaaccgcgat ggccggcgag gccgacacga
     6721 gcttcgacgt caagggtggc ggctgctcgg tgtccggtga ctcgaaccag ctcatcggca
     6781 acgtcggcgt tctgaacggc gtcgtcggtg gcattctgac cttcggcaac gacggtgacc
     6841 acgggaccca gggcaacgag cagggccaca aggtcgactg ctccacccgc gggttcaccg
     6901 gctgacgccg agtagacgca tgaggacagg tcccggggct cgccccgggg cctctccccg
     6961 acctttctca caatcccgag cgttgatctc gcgccaactc gtcaaggggc acgcaggcag
     7021 tggccgcacg gttcgagacg cccagcgcct ggggggacct accgcaaagg acaaagcgga
     7081 tgcagaattt gaagaagatc gcagtggtgg cgggtttcct gggaggctcc gttctgatgg
     7141 gcctcggtac tgcccaggcc cagagtttcg acatccccgt ctgtgacgag aaggccagcg
     7201 acgggcactg cgggcagaag cgtgaatatc agtacaccac tgaaaacggt cagcacgtcc
     7261 atgttgtcgt cgcgcgtgaa ggctgtgcga tcaaggggaa cagcaccgtc gactgctcca
     7321 ttcaccaccc ctgacccgct cgaggcaatg gcgcccgctc gacgcagccg tgattcctgc
     7381 tgaggtacgc ctggctcccg ccgcagcgtc atgggcagcg gctgctccac aagcctgatg
     7441 gccggacccg cctttccggg gccggccatc aggcgtgtac gcggcgcgat ggacgcggaa
     7501 ctgctccgcc atgagtgcgc ggttttgccg accggccttc gtgatctagc ttcgtgatct
     7561 tcgacgttac gctgacgccg gtccatcgga tggccgctga catcccgtac cactccgggg
     7621 acacgcgcca cggtatgcac gtccggatcc ttgccgcccc gttcggccgt ccgccgaggg
     7681 cttcaccggc cttgcccggt tccgttcacg agtgggccgc tgtagcgcag accggatgac
     7741 cggcctcgtg cggtccgtcc tcacccctcc cctccgaacg gccccgcgcc ggccttggcc
     7801 ggtcgccgag ggcgccgccc gccatgcggg gcgtccggcc tgcggccgtc actgacctgc
     7861 cgagtgcggc caagtgaacg aagcggtgcc tcagcgacac cccgccgccg cgacagggcc
     7921 tgtgcgcggt gactccatcg gggtgcagta cggcagatcg tcgatggggt cgtccgtgca
     7981 gaaccggccg atcaggtccg cggactcctg cctgcgttcg accgtgacca ggtggtcggc
     8041 ctctttgatg gtcgtgaacc gcgctgcggg catcgccgcg gcgagctcgc ggcccatctg
     8101 cggggtgcac agggtgtcgt actctccggt gaacaccagg gacggcacgt cggggacggg
     8161 cagaggctgg tagcaaccgt gcgtcaaaag cctcgtgttg tgctcgaccg ccttcgtgat
     8221 ctcatccggc gtctggtcca ggaactgccg ctggaggaga cgcgacacgg ccgcgtgctt
     8281 gcgaacccga ccggcgctcg ggttcgacat gaacaaccgc accagcccgt cggcggcctc
     8341 ttcgatcctg ccgcgttcga gtgcgtgcga caggcgcggt acggcggcgg tgtagatctc
     8401 gggaaggcgc ttcgccgcgc cgcagaagat gatgcgactg acgaactcgg ggtagagctg
     8461 cgcgaagcgg aggccgacga cctcaccgaa gcagccgccg aagaggttca ctcgcggcat
     8521 tttgatctcc accagcatgt gcttgaccgc ggaggcgagg aagtcgatgc cgtatcgagc
     8581 gggcaggaag tccgcggtgc cgtagccggg aagatccatc gtgatcaacg tgcacagcgg
     8641 tgccaggtac ttctcgtgac cctgccatga gtacttgttc tgggacgagc cgccgagaag
     8701 caacagcggt tccgtccgcg gcgcacgctg atggacaatg cggcagtcgt acgcgaaacc
     8761 gtcgacaacg aggccgcgtt cctccacgcg ttccggcggt gcggcaacag gccgggagcg
     8821 ctctgcggtg agttcctggg tgtcggtgac ggcagtgcgc atggagactc cggtaaagac
     8881 gaagggcagc gagccgttcc atcattacca agagtcccac caacctgatg taatcactca
     8941 attgtgggcg atcccggctg aagtcgctta cctgcgcagt ggttaccgcc agtgctgtgg
     9001 gtctgctgag gtctcacggc atcgagacag tgaccggttt ccgggggtcg ggggccggag
     9061 tcgaccgcca cgggccgaag cggcgttgcc gggaacgacg gaccgcgcgg tggcggactg
     9121 gcgggaacgg cggaccgcgc ggtggcggac tggcgggacc gccgaaaccg gtcgaggccc
     9181 ggcggggcgc ggtatcccgc gctttcgggt gtaggcgccc ccggcaggac tcgaacctgc
     9241 ggccaagcgc ttagaaggcg cctgctctat ccactgagct acgggggcca ggtgtggtgg
     9301 cctcggtcgt ggtgcccggg tgtgggctga accgtgacac tgccggggac aaggataggg
     9361 ctcccggctc catgtccctg atgcttcgct tccgtgcctc gatgtggagg ttcagtgaag
     9421 cgatcctgat aatcgcaggt aggtacgaat cgtgcatcgc ttttggcatc tcgcgcatcg
     9481 ggtgttgtcc actcgttatg cctggactgc tcacgcgtcc cagtagtccc ttccgtctgg
     9541 agtgtccggt cggcgcgcag gcatactcat atgcttcaaa attcccctaa aattgggcat
     9601 tcttcacatg tggtgacctt ggacgtacgg cctcagctgc tcgacgcact ctccgccctg
     9661 cgcgaccgtg tcgcggccgc acgcttcccg ctgcccctgg cgggggcccc acgcgcgcgt
     9721 gccaaccgcg acgaactgct cgcacagctc gacgactact tggtgccccg gttgaaggag
     9781 cccgaagcgc ccttgctggc cgtggtgggc ggctccaccg gcgccgggaa gtccacgctg
     9841 gtgaactccc tcgtggggcg gcgggtcagc gaggcggggg tgctgcggcc cacgacccgg
     9901 acgccggtgc tggtgtgcca tccggaggac caccactggt tcagcggcat gcgggtgctg
     9961 cccgacctca cgcgcgtgtg ggtgccccac cgggaaccgg acgacgacct gctcctgccg
    10021 ggcgagaacc ccgcgcgcgt actgcgcgtc gagaccgccg agaccctgcc gcccggcatc
    10081 gccctcctgg acgcgcccga catcgactcc ctcgtcgccg acaaccgcgt cctggcggcc
    10141 gaactgctct gcgccgccga catctgggtg atggtcacca cagccgcccg gtacgccgac
    10201 gccgtgccct ggcacctgct gcgcaccgcc aaggagtacg acgccaccct ggtcaccgtg
    10261 ctcgaccggg tgccccacca ggtggtgtcg gaggtgtcgc ggcagtacgg ggcgctgctc
    10321 accaaggccg ggctcggcga ggtgccccgc ttcaccgtgc ccgagctgcc ggagtcggcc
    10381 tggggcggcg ggctgctgcc ggccagcgcg gtggcgccgc tgcggtcctg gctcgtgcac
    10441 cgcgcgcagg atctcgacgc ccgccaccac gccatggccc gtacggcgca cggcatcctg
    10501 gactccc
//
