LOCUS       NZ_CP019724            10288 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Streptomyces pactum strain ACT12 chromosome, complete genome.
ACCESSION   NZ_CP019724
VERSION     NZ_CP019724.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces pactum
  ORGANISM  Streptomyces pactum
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Streptomyces pactum ACT12 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2017) College of Life Sciences, Northwest A&F
            Unversity, No.22 Xinong Road, YangLing, Shaanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2016
            Assembly Method                   :: SMRT v. 2.3.0
            Assembly Name :: version 2.3.0 of the PacBio Single Molecule Real
            Time
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002005225.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:58:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,315
            CDSs (total)                      :: 7,221
            Genes (coding)                    :: 6,992
            CDSs (with protein)               :: 6,992
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 84 of 229
            Pseudo Genes (incomplete)         :: 195 of 229
            Pseudo Genes (internal stop)      :: 15 of 229
            Pseudo Genes (multiple problems)  :: 62 of 229
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:20:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4293137
            Orig. end                         :: 4303425
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019724.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Northwest A&F Unversity.
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="24"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5288](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5288)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    433..448
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.61"
                     /tool="antismash"
     gene            complement(569..2044)
                     /locus_tag="B1H29_RS18100"
                     /old_locus_tag="B1H29_18145"
     CDS             complement(569..2044)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356876.1"
                     /locus_tag="B1H29_RS18100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_18145"
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="WP_079160296.1"
                     /transl_table=11
                     /translation="MRLRTASMPVPLWRPPVWPVPVLWTLALGLWGLSRQHSVWRDEAA
                     TWQVARRPAADIWHMLGQVDVVHGLYYLLMHGLFACFGPGTTTLRLPSVLAMAVAAACV
                     TVIGHRLAGVWAGLGGGLALGLLPAVQFYLQEGRPYALTAAGAGISTLLLVTLLQGRGR
                     TAHWAGYAGAVLVCGLLNWLSLLILPAHAATLLWTRAGRDTWARWAVASAAAAAGVLPL
                     ILFSRQQSRQVSWIPSLTWHMLIGPAVLLTIGAVGALLNRPKAGRLSVAAVGLPLLAVP
                     QLALAGLSLIRPLFLDRYVLFSMLGLALLVGAGLSAAVRAVGPRYPRASSWLVPAVVAV
                     AVVALLPQSLAKRSPASRVDDVLASAADVRRLKRPGDAVVFIPAARRDAALVCPADFAG
                     LRDVALEESPVASGTLKGVEAGPARIRTAMLAQRRILLVTDAPGVARPRSAGRDKAKAA
                     VLEEYFTVVADEQVRGRRLTVYERRSRADGVAR"
     gene            complement(2062..2706)
                     /locus_tag="B1H29_RS18105"
                     /old_locus_tag="B1H29_18150"
     CDS             complement(2062..2706)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374867.1"
                     /locus_tag="B1H29_RS18105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_18150"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_055418867.1"
                     /transl_table=11
                     /translation="MIEIVKLTKTFGTRTLWSGVTVNADRGRMLALVGPSGSGKSTLLN
                     CVGLLDSPTAGEIRHEGADITRFGRQEARRFRRDTLGYLFQNYALIDNATVADNLEVSL
                     STRRARREKSGARIAEALERVGLAGREKERVHRLSGGEQQRVALARLLVKKPSLVLADE
                     PTGALDHANATAVVDILREMSDAGCAVVIATHDDRVRDRCDSVFAVHEKSL"
     misc_feature    complement(2679..2699)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.83"
                     /tool="antismash"
     gene            complement(2750..4867)
                     /locus_tag="B1H29_RS18110"
                     /old_locus_tag="B1H29_18155"
     CDS             complement(2750..4867)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B1H29_RS18110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B1H29_18155"
                     /product="hypothetical protein"
                     /protein_id="WP_055418868.1"
                     /transl_table=11
                     /translation="MLHRGIKFVHAAVLVTSAMMAFLFVRGLDEQLVLGNSAQVWVFAS
                     DDSASGARVAREIAAFSDEHAVSVAREVPDARNPDGLRHLYLASGDPRSDAASWQYGGY
                     PDFSRHLDTRVHPITEIGQRDPRGFYYVFGPPEAADALVAEFTGLGLSASVRHPLSAGE
                     LALAFSGDDLFWTFWVVALAAATMTGASVLLSAKAYGVLRLQGKSVRDLLVRDLGRLAG
                     FWSITVCAVAAATVALLGLYNGLARAGLFASVAGILAGLLVVLVLVTHAGALVLLSRVG
                     IPRALKGELPARAALIATYLVRVPALLLALAIAMDVALAGQNVVARQDTQGTYARAGDA
                     VTIRLNGSLVGEEKEVVTQVGGWLRRADAAGEIIVAGRRDLQDVSSDVRLPQGELLVVN
                     ETFLARQPVLDSTGRRYTALPRDGENADAPAVRLIVPDSLARHTADIRAGIPDVFGRLA
                     PDVRRALEVKSLRARNGQRLFGYNPGSLSPNTAHRPDDDRSLVRDPVLVVVPNGSRFLS
                     DDTYTAFASQEGIVFPDPDDALEAIAADRNGLRTYVTSVRPVGQNAALRLRDAVNGFRL
                     QLFNLVAAAAVLLITGVGVCMLHARRRAQTIFVRHISGWRFTANHRSVLTAEGILAVLL
                     TCWVPVQAWRQNQELAEFTARGIPAPFPAVQTTASDFAVIAGLVAVQVCAVLVALGVFH
                     RRVVKEGAAGS"
     misc_feature    4906..4917
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.75"
                     /tool="antismash"
     gene            complement(5001..5288)
                     /locus_tag="B1H29_RS18115"
                     /old_locus_tag="B1H29_18160"
     CDS             complement(5001..5288)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="B1H29_RS18115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_18160"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_055418869.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.1e-11,
                     bitscore: 43.0, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNGLKAAAAAGAIVMAVATPALAKSVDVSGGRWSYDVGANYAYS
                     NFYHSVNYHSSSVHGKSWAYSGCTANKKWSKASVEKSSIHVNSAHYDAAC"
     misc_feature    5286..5299
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     gene            complement(5359..5634)
                     /locus_tag="B1H29_RS18120"
                     /old_locus_tag="B1H29_18165"
     CDS             complement(5359..5634)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763151.1"
                     /locus_tag="B1H29_RS18120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_18165"
                     /product="hypothetical protein"
                     /protein_id="WP_055418870.1"
                     /transl_table=11
                     /translation="MLVIGPLVTTAIAIVLPLSVAVPAEAARGWTAHFQCTKKRISDDT
                     AHGFVQGHGTGKTASEAKKAAKKNANDQMPRGYRAKHCTQKSVKRL"
     gene            6043..6477
                     /locus_tag="B1H29_RS18125"
                     /old_locus_tag="B1H29_18170"
     CDS             6043..6477
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521577.1"
                     /locus_tag="B1H29_RS18125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_18170"
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="WP_055418871.1"
                     /transl_table=11
                     /translation="MKQRQQKKLSRHLFEAILLGDTARVRTMLRAGADPERADGEGTLP
                     LYEAAVSGKTEIVRLLLDSGASPDAESGGIGAEGTPLCAAACWGHTETVRELLAHGADA
                     GLREDHGTGRSPLDWANEGPHPETAALLIAAGAAPRTAGG"
     misc_feature    6059..6072
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.21"
                     /tool="antismash"
     gene            complement(6585..7556)
                     /locus_tag="B1H29_RS18130"
                     /old_locus_tag="B1H29_18175"
     CDS             complement(6585..7556)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1208: pirin
                     domain-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014673857.1"
                     /locus_tag="B1H29_RS18130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_18175"
                     /product="pirin family protein"
                     /protein_id="WP_055418872.1"
                     /transl_table=11
                     /translation="MPAVTVENPLTLPRVSAPADAVARPVLTVTTAPSGFEGEGFPVRR
                     AFAGINYRHLDPFIMMDQMGEVEYQPGEPKGTPWHPHRGFETVTYIIDGIFDHQDSNGG
                     GGTITNGDTQWMTAGSGLLHIEAPPEQLVMSGGLFHGLQLWVNLPARDKMMAPRYQDIR
                     SGSVQLLTSPDGGALLRVIAGELDGHEGPGITHTPITMVHATLAPGAELTLPWREDFNG
                     LAYVMAGRGSVGAERRPVHLGQTAVFGAGGSLTVRADGKQDSHTPDLEVVLLGGQPIRE
                     PMAHYGPFVMNTREELQQAFEDFQKGRLGTVPAVHGMTEGGL"
     misc_feature    complement(7609..7623)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.32"
                     /tool="antismash"
     gene            complement(7678..8151)
                     /locus_tag="B1H29_RS18135"
                     /old_locus_tag="B1H29_18180"
     CDS             complement(7678..8151)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985816.1"
                     /locus_tag="B1H29_RS18135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_18180"
                     /product="SseB family protein"
                     /protein_id="WP_055418873.1"
                     /transl_table=11
                     /translation="MYGYDQTAGQQQQQYAPPQQPMSGGYGQQQQPPLYPEPSPPSLAD
                     AVRAFTTGQMSAEDFQQIFATSKVYCPRGDNPGFLALHNTQQPVIPMFTGLKELRRYAG
                     KESKYFVITGAEVIDLLPTGYGFVLDMEGEHRMVFDAKAVEQMVDFAMRRMYG"
     gene            8314..10140
                     /locus_tag="B1H29_RS18140"
                     /old_locus_tag="B1H29_18185"
     CDS             8314..10140
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991606.1"
                     /locus_tag="B1H29_RS18140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_18185"
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="WP_055418874.1"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 9.6e-19, bitscore:
                     66.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 3.3e-16, bitscore:
                     58.5, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGHYKPNLRDIEFNLFEVLGRDKVYGTGPFAEMDTDTAKSVLEEL
                     TRLSENELAESFADADRNPPVFDPETNTAPVPASFKKSYQAFMDSEYWRLGLPEEIGGT
                     TSPRSLIWAYAELILGANPAVWMYSSGPAFAGILFEEGNEVQKHIASFAVEKQWGSTMV
                     LTEPDAGSDVGAGRTKAVQQEDGSWHIEGVKRFITSGEHDMSENILHYVLARPEGAGPG
                     TKGLSLFLVPKYEFDFETGELGARNGVYATNVEHKMGLKASNTCEMTFGDRHPAKGWLI
                     GDKHDGIRQMFRIIEFARMMVGTKAISTLSTGYLNALEYAKERVQGPDLANFMDKSAPK
                     VTITHHPDVRRSLMTQKAYAEGMRALVMYTASVQDEIQVKEANGEDAATEHALNDLLLP
                     IVKGYGSEKAYEQLAQSLQTFGGSGFLQEYPIEQYIRDAKIDTLYEGTTAIQGQDYFFR
                     KIVRNQGAALNSLAEDIKKFLALGTGGEELSGAREHLAKAAVELEAIVGLMLTDLAATE
                     QDVKNIYKVGLNTTRLLMASGDVIVGYLLLKGAAIAAEKLPTASAKDKAFYTGKIAAAK
                     FFAASVLPGVTMARKIAQGVELDLMELDEAAF"
ORIGIN
        1 aggaagaaga agacgagcag cagggcgagt acggccatgg ccagcatctc gccgacgacg
       61 ctgatcccgg tgaccacccc ggaggccgcc gtgccgccga acttgcccag cagctcccgc
      121 gcgttggacg cgatgtcgtc cagcgaggtc ccggccgctc cgaaatggtc ggtgatcccc
      181 ttggccgcgt gccggagcga ggcgatgatc tggtcgcccg tctcgatgag cgcggacacc
      241 acgatgtaca cggccccgcc gaccacggcg acgacggcga cacaggtgag tccggcggcc
      301 agcgaccgct gcacgtgcgc cttcaccagc cacttgtgca gcggtctgag cagtgcggtg
      361 ccgagcagcg ccagcagcgc cggcgtcacc gcggtgcgga actcgacgca gagccggatc
      421 gcgacgtaca cgacgccgct ggcgagcagg atgacgacgc accaggcggc gaaccgacgg
      481 accggaccgg gaaggagctg cacgccccca gccgaacacg caccgggcgg gctgtcctgc
      541 gtggacggcc cgcccgggtg acacggcgtc agcgcgccac cccgtcggcc cgcgaccgcc
      601 gctcgtacac cgtcagccgt cgcccgcgga cctgctcgtc cgccaccacg gtgaagtact
      661 cctccagtac cgcggccttg gccttgtccc gccccgccga ccgcggccgg gccaccccgg
      721 gcgcgtcggt gaccagcagg atgcgccgct gtgccagcat cgcggtccgt atccgggccg
      781 gaccggcctc cacccccttc agcgtcccgg acgccacggg gctctcctcg agcgcgacgt
      841 cccgcagtcc cgcgaagtct gcggggcaga ccagcgcggc gtcacggcgg gccgccggta
      901 tgaagaccac cgcgtcgccg ggccgcttca gccgccgtac atccgccgct gacgccagga
      961 cgtcgtcgac ccggctggcc ggggaccgct tggccagcga ctgcggcaac aacgccacca
     1021 ccgcgacggc gaccaccgcg ggtacgagcc acgacgacgc cctcgggtac cgcggcccga
     1081 cggcccggac cgccgcgctc aggcccgccc cgaccagcag cgccagaccc agcatgctga
     1141 acaggacgta gcggtccagg aacaggggac ggatcaggga gaggcccgcc agcgcgagct
     1201 gcggcaccgc cagcagcggc aggccgacgg ccgccaccga caaccggccc gccttcggcc
     1261 ggttcagcag ggcaccgacg gcgccgatcg tcagcaggac cgccgggccg atcagcatgt
     1321 gccaggtcag cgaaggtatc caggacacct gacgggactg ctgccggctg aagaggatca
     1381 acggcaggac accggccgcg gcggccgccg aggccaccgc ccagcgcgcc caggtgtccc
     1441 gtccggcccg cgtccacagc agcgtcgccg cgtgcgcggg caggatcagc agcgacagcc
     1501 agttcagcag cccgcacacc aggaccgcgc cggcgtagcc cgcccagtgc gccgttcggc
     1561 cacgcccctg gagcagcgtc accagcagca gcgtcgagat ccccgcgccg gccgccgtca
     1621 gcgcgtacgg gcggccctcc tgcaggtaga actgcacggc cgggagcagc cccaacgcca
     1681 ggccgccgcc caggcccgcc cagactccgg ccagcctgtg gccgatgacc gtcacacagg
     1741 cggccgccac cgccatggcc agcacggagg gcagccgcag ggtcgtcgtg ccgggcccga
     1801 agcatgcgaa gaggccgtgc atcagcaggt agtagagccc gtgcacgaca tcgacctggc
     1861 cgagcatgtg ccatatgtcg gcggccggcc gccgggccac ctgccaggtc gcggcctcgt
     1921 cccgccacac gctgtgctgc cgggacagcc cccacaggcc gagggcgagg gtccacagca
     1981 ccgggaccgg ccagaccggc ggacgccaca gcggaacggg catggaggcg gtccgcaaac
     2041 gcatgacgct tctcgaaccc ctcacaggga tttctcgtgg acggcgaaaa cggagtcgca
     2101 gcggtcacgg acgcggtcgt catgggtggc gatgaccacg gcgcatccgg cgtcgctcat
     2161 ctcgcggagg atgtcgacga ccgcggtcgc gttggcgtgg tcgagggcgc cggtcggctc
     2221 gtcggccagt accagggagg gctttttcac gaggagccgg gcgagcgcca cacgctgctg
     2281 ttcgccgccg ctgagccggt ggaccctctc cttttcccgg cccgccagac cgacacgctc
     2341 cagggcctcc gcgatgcgcg cgccgctctt ctcccgccgg gcccgccgcg tgctgagcga
     2401 gacctccagg ttgtcggcga cggtggcgtt gtcgatcagc gcgtagttct ggaagaggta
     2461 gccgagggtg tcacggcgga accgacgggc ctcctgccgg ccgaaccggg tgatgtcggc
     2521 gccctcgtga cggatttctc cggcggtcgg actgtcgagg aggccgacgc agttgaggag
     2581 ggtggacttc cccgatccgc tgggtccgac gagggccagc atgcggccgc ggtcggcgtt
     2641 gacggtgaca ccggaccaca gggtgcgggt gccgaaggtc ttcgtcagct tcacgatctc
     2701 gatcacaacg gtgctcctgc tgtccggagg tggttcgtct gcgtggggct catgaaccgg
     2761 ccgccccctc cttgacgacg cggcggtgga acaccccgag ggcgaccagc acggcgcaga
     2821 cctggacggc gacgagaccg gcgatgacgg cgaagtccga cgcggtcgtc tggacggccg
     2881 ggaagggagc ggggatgccg cgggcggtga attccgccag ctcctggttc tgccgccagg
     2941 cctggacggg gacccagcag gtgaggagga cggcgagaat cccctcggcg gtcaggacgg
     3001 agcgatggtt cgcggtgaat ctccagccgc tgatgtgccg gacgaagatg gtctgggcac
     3061 gccggcgcgc gtgcagcatg cagaccccga cgccggtgat gaggagcacg gccgcggccg
     3121 cgacgagatt gaagagctgc agccggaagc cgttcaccgc gtccctgagt ctcagtgcgg
     3181 cgttctgccc gaccggacgc acggatgtca cgtaggtccg cagtccgttc ctgtccgccg
     3241 cgatcgcttc cagggcgtcg tcggggtcgg gaaagacgat tccctcctgg ctggcgaacg
     3301 cggtgtaggt gtcgtcggag aggaatcgcg aaccgttggg gaccacgacg aggaccgggt
     3361 cgcgcaccaa ggaccggtcg tcgtccgggc gatgggccgt gttggggctc agactgccgg
     3421 ggttgtagcc gaagaggcgc tggccgttcc tcgcccgcag cgacttcacc tcgagcgcgc
     3481 ggcggacatc cggtgcgagt cgtccgaaca cgtcggggat gcccgcgcgg atgtccgccg
     3541 tgtggcgggc gagggagtcg gggacgatga gccggaccgc gggggcgtcc gcgttctccc
     3601 cgtcccgcgg cagtgcggtg taccgccgtc cggtcgagtc gaggaccggc tgccgggcga
     3661 ggaaggtctc gttgacgacg agcagttcgc cctgcggaag acggacgtcg gaggagacgt
     3721 cctggaggtc acggcggccg gcgacgatga tctcgcccgc ggcgtcggcg cggcgcagcc
     3781 acccgcccac ctgggtgacg acctccttct cctcgcccac caagctgccg ttgaggcgga
     3841 tggtgacggc gtcgcccgcc cgggcgtagg tgccctgggt gtcctgccgg gccacgacgt
     3901 tctgccccgc gagcgccacg tccatggcga tggcgagagc gagcagcaac gccgggacac
     3961 ggaccaggta cgtggcgatg agcgccgccc gcgcgggcag ctcccccttg agggcgcgcg
     4021 gtatcccgac cctggagagc agcacgagcg cgcccgcgtg cgtgaccagc accaggacca
     4081 cgagcaggcc ggcgaggatc ccggccaccg acgcgaacag ccccgcccgg gcgagaccgt
     4141 tgtagaggcc gaggagggcc accgtagcgg ccgccaccgc gcagacggtg atcgaccaga
     4201 aacccgccag ccgtccgagg tcgcgaacga gcaggtcccg cacggacttg ccctgcaggc
     4261 gcagcactcc gtacgccttg gcactcagca gtacgctcgc gccggtcatc gtcgccgcgg
     4321 cgagggcgac cacccagaag gtccagaaca ggtcgtcgcc cgagaatgcg agggccagtt
     4381 cgccggccga gagcgggtgc cgcacgctcg cgctcaggcc cagtccggtg aactcggcga
     4441 ccaacgcgtc agcggcctcg ggcggtccga aaacgtagta gaagccccgc gggtcacgct
     4501 gcccgatctc ggtgatcgga tgcactcgcg tgtcgaggtg cctgctgaag tccgggtagc
     4561 cgccgtactg ccacgaggcg gcgtcggacc gcgggtcgcc cgaggccaga tagaggtgcc
     4621 gcagcccgtc ggggtttctc gcgtcgggga cctcacgagc caccgagacc gcgtgctcgt
     4681 ccgagaacgc ggcgatctcg cgggcgaccc gagcgccgct cgcggagtcg tcggaggcga
     4741 acacccagac ctgcgccgaa ttgcccagca ccaactgctc gtccaggccc cgcacgaaca
     4801 ggaaggccat catcgcggag gtgaccagca cggcggcgtg gacgaatttg atacctcggt
     4861 ggagcatcgg ttacctaggt cagggcactc ggggagcgca ttccagaaaa cgattgacgc
     4921 ggaggggcga ctgcggggag aaagcggtct ccccgcagcc ggatcccggc taccgactca
     4981 acgcggcgta cggcggcctg tcagcaggcg gcgtcgtagt gagcgctgtt gacgtgaatg
     5041 ctcgacttct ccaccgaggc cttggaccac ttcttgttcg cggtgcagcc gctgtaggcc
     5101 cagcttttcc cgtgcacgga ggacgagtgg tagttgacgc tgtgatagaa gttcgagtag
     5161 gcgtagttcg cgcccacgtc atagctccac cggccgccac tgacgtcgac cgacttggcc
     5221 agcgcggggg tggcgacggc catgacgatg gcaccggcgg cggctgcggc cttgagaccg
     5281 ttcttcatac gcacgattgc tcactttcgt tctgtgcttg cttgacgcga agggggcaga
     5341 agtcgtcccg cccacggatc agagcctctt gaccgacttc tgggtgcagt gcttcgcgcg
     5401 gtacccgcgg ggcatctggt cattggcgtt cttcttggcc gccttcttgg cctccgacgc
     5461 cgtcttcccg gtgccgtgcc cctgcacgaa cccgtgggcg gtgtcgtccg agatgcgctt
     5521 cttggtgcac tggaagtgcg cggtccagcc ccgcgccgct tcggcgggaa cggcgacgct
     5581 caaaggaagc acgatcgcta tggccgtggt gacgagcggg ccgatgacaa gcatcctgcg
     5641 caagagtatt tctcccccat tcaaattcgc atgcggcggg ggcaggccgc gcggtgttgc
     5701 agtacaccac cgttcacccg ctgcagcggg cacaccaaaa cacgccttgg ccgtgcggcc
     5761 aatgcgggat ttgagtattg ctctcgcccc cgtgaaaacc actcgtctcg gaggtcagcc
     5821 cgatcgagtg gacgtgccag aagttacgcg gcacggacgc actttatcca gccataacga
     5881 ggtgatcatg atcacctggc cggcaggcaa cccggaagac acccggtggc ccgattggag
     5941 aagctgagcc gcattgcgtc ctttgcgtcc gtaatggccc gagcccccgg cgagcggata
     6001 tgaatggctc gtaccactcc ccgtccgcta gcctccacag ccatgaagca gcggcagcag
     6061 aagaaacttt cccgccacct cttcgaagcg attctgctgg gcgacaccgc ccgagtgcgg
     6121 acgatgctgc gggccggtgc ggaccccgag cgggccgacg gcgagggcac cctccccctg
     6181 tacgaggccg ccgtgagcgg gaagaccgag atcgtgcgtc tgctgctgga ctcgggcgcc
     6241 tcccccgacg ccgagagcgg gggaatcggc gcggagggca ctccgttgtg cgcggcggcg
     6301 tgctgggggc acacggagac cgtgcgggag ttgctggcgc acggagccga tgcggggctc
     6361 cgagaggatc acggcaccgg ccgttccccc ctggactggg cgaacgaggg cccgcacccc
     6421 gagaccgccg ccctcctgat cgcggcgggg gcggcgcccc gcacggcggg cgggtagccg
     6481 ccggctcccc gcctggcatg ggaaagcccc gaccggcacc ggtcggggct ttcacgcgct
     6541 cgggccctga cggcctgggc cgcccgcgct cgggcctcga aggcctacag gccgccctcg
     6601 gtcatcccgt gaaccgccgg gaccgtcccc aggcgcccct tctggaagtc ctcgaaggcc
     6661 tgctggagtt cctcgcgggt gttcatgacg aacggcccgt agtgcgccat cggctcccgg
     6721 atcggctgcc cgccgagcag cacgacctcc aggtcgggcg tgtgggagtc ctgcttcccg
     6781 tccgcgcgga cggtcagcga gccgccggcc ccgaagacgg cggtctgccc caggtggacc
     6841 gggcgccgct cggcgcccac cgacccgcgt ccggccatca cgtacgccag gccgttgaag
     6901 tcctcccgcc acggcagggt gagctccgcg ccgggtgcca gcgtcgcgtg gaccatcgtg
     6961 atcggcgtgt gcgtgatgcc ggggccctcg tggccgtcca gctcaccggc gatgacacgc
     7021 agcagcgcgc cgccgtcggg ggaggtgagc agttggacgg agccactgcg gatgtcctgg
     7081 tacctggggg ccatcatctt gtccctggcc gggaggttca cccacagttg gaggccgtgg
     7141 aagagcccgc cggacatgac gagctgctcc gggggcgcct cgatgtgcag caggccggag
     7201 cccgccgtca tccactgggt gtcgccgttg gtgatggtgc cgccgccgcc gttggagtcc
     7261 tggtggtcga agatcccgtc gatgatgtac gtgacggttt cgaagccgcg gtgggggtgc
     7321 cacggggtcc ctttcggctc cccgggctga tactcgacct cacccatctg gtccatcatg
     7381 atgaacgggt cgaggtggcg gtagttgatc ccggcgaacg cgcggcgaac cgggaagccc
     7441 tcgccctcga aaccgctggg cgcggtcgtg acggtgagca cgggacgtgc gacggcgtcg
     7501 gctggtgcgg atacacgggg cagcgtcaac gggttctcga cggtcactgc aggcatgtcg
     7561 gtacctcctc gggtacgccc agtttagttg agcgttgaac ttcttgccac ctcaaaccga
     7621 gaaagcccgg agggcattcc ctccgggcgc gcgaagcggg acgcgacgcg tcggcgccta
     7681 gccatacatg cggcgcatcg cgaaatcgac catctgttcg acagccttcg cgtcgaacac
     7741 catccggtgc tcaccctcca tgtcgagtac gaatccgtag ccggtcggca gcagatcgat
     7801 cacctcggcg cccgtgatca cgaagtactt ggactccttg cccgcgtatc ggcgcagctc
     7861 cttgagcccc gtgaacatcg ggatcaccgg ctgctgggtg ttgtgcagcg cgaggaagcc
     7921 cgggttgtca ccgcgcgggc agtagacctt cgaggtcgcg aagatctgct ggaagtcctc
     7981 ggccgacatc tggccggtgg tgaaggcgcg caccgcgtcc gcgagcgagg gcggggacgg
     8041 ctcggggtac agcggcggct gctgctgctg cccgtacccg ccggacatcg gctgctgcgg
     8101 cggggcgtac tgctgctgct gttggcccgc ggtctggtcg tagccgtaca tgccgcaaag
     8161 agtagcgagt gacgggtcac agatgaccgg ctcggggttg cttcttatta ccgacgggta
     8221 gcatcatcgt agctactgct tggtacgcgc agtcggtatg tgtacagatg cgaggattca
     8281 gtgcctcgcc gatctctcaa cggagccgtc gccatggggc actacaagcc gaatctccgg
     8341 gacatcgagt tcaacctctt cgaggtactc ggccgtgaca aggtgtacgg cacgggcccg
     8401 ttcgcggaga tggacacgga caccgccaag agcgtcctgg aggagctgac ccgcctctcg
     8461 gagaacgagc tggccgagtc cttcgccgac gccgaccgca acccgccggt cttcgacccg
     8521 gagaccaaca ccgcgccggt cccggcctcc ttcaagaaga gctatcaggc cttcatggac
     8581 tccgagtact ggcgcctggg cctgcccgag gagatcggcg gcaccacctc gccccgctcc
     8641 ctgatctggg cctacgcgga gctgatcctc ggcgccaacc cggccgtgtg gatgtactcc
     8701 tccggccccg ccttcgcggg catcctcttc gaggagggca acgaggtgca gaagcacatc
     8761 gcgtccttcg ccgtggagaa gcagtggggc tccaccatgg tcctcaccga gccggacgcc
     8821 ggctcggacg tcggcgccgg ccgcaccaag gcggtccagc aggaggacgg ctcctggcac
     8881 atcgagggcg tcaagcgctt catcacgtcc ggcgagcacg acatgtcgga gaacatcctc
     8941 cactacgtcc tcgcccgccc cgagggcgcc ggccccggca ccaagggcct gtccctcttc
     9001 ctcgtcccga agtacgagtt cgacttcgag accggcgagc tgggcgcgcg caacggcgtc
     9061 tacgccacca acgtcgagca caagatgggc ctgaaggcct ccaacacctg cgagatgacc
     9121 ttcggcgacc gccaccccgc caagggctgg ctgatcggcg acaagcacga cggcatccgc
     9181 cagatgttcc gcatcatcga gttcgcccgc atgatggtcg gcacgaaggc gatctccacc
     9241 ctctccacgg gctacctcaa cgccctggag tacgccaagg agcgcgtcca gggtcccgac
     9301 ctggccaact tcatggacaa gtcggccccc aaggtcacca tcacgcacca cccggacgtc
     9361 cgccgctccc tcatgacgca gaaggcgtac gccgagggca tgcgcgccct cgtcatgtac
     9421 accgcctccg tccaggacga gatccaggtc aaggaggcga acggcgagga cgccgccacc
     9481 gagcacgccc tcaacgacct gctcctgccg atcgtcaagg gctacggctc ggagaaggcc
     9541 tacgagcagc tcgcccagtc gctccagacc ttcggcggct ccggcttcct gcaggagtac
     9601 ccgatcgagc agtacatccg ggacgccaag atcgacaccc tgtacgaggg caccacggcg
     9661 atccagggcc aggactactt cttccggaag atcgtccgca accagggcgc cgcgctgaac
     9721 tcgctcgccg aggacatcaa gaagttcctg gccctcggca ccggcggcga ggagctctcc
     9781 ggcgcccgcg agcacctggc caaggccgcc gtcgagctgg aggccatcgt cggcctcatg
     9841 ctcaccgacc tcgcggccac cgagcaggac gtcaagaaca tctacaaggt ggggctcaac
     9901 accacccgcc tgctgatggc ctccggtgac gtgatcgtcg gctacctgct gctcaagggc
     9961 gccgccatcg ccgccgagaa gctgccgacg gcgtccgcca aggacaaggc gttctacacc
    10021 ggcaagatcg ccgcggcgaa gttcttcgcg gcgagtgtcc tgcccggcgt caccatggcc
    10081 cgcaagatcg cccagggtgt cgagctggac ctgatggagc tggacgaggc cgccttctag
    10141 cccaccgggg ctcggcctcc acactctcca cacaccctta cgagggcccg ctcccgctcc
    10201 acgggaagcg ggccctcgta cgtcgttaag gtgaacccat gagcgcaccc tcccgcccgt
    10261 ctcccgccgc cgcccccgca cggagcgc
//
