LOCUS       NZ_CP019724            10216 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Streptomyces pactum strain ACT12 chromosome, complete genome.
ACCESSION   NZ_CP019724
VERSION     NZ_CP019724.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces pactum
  ORGANISM  Streptomyces pactum
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Streptomyces pactum ACT12 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2017) College of Life Sciences, Northwest A&F
            Unversity, No.22 Xinong Road, YangLing, Shaanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2016
            Assembly Method                   :: SMRT v. 2.3.0
            Assembly Name :: version 2.3.0 of the PacBio Single Molecule Real
            Time
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002005225.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:58:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,315
            CDSs (total)                      :: 7,221
            Genes (coding)                    :: 6,992
            CDSs (with protein)               :: 6,992
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 84 of 229
            Pseudo Genes (incomplete)         :: 195 of 229
            Pseudo Genes (internal stop)      :: 15 of 229
            Pseudo Genes (multiple problems)  :: 62 of 229
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:20:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 719209
            Orig. end                         :: 729425
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019724.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Northwest A&F Unversity.
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    169..183
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     gene            605..2746
                     /locus_tag="B1H29_RS03190"
                     /old_locus_tag="B1H29_03200"
     CDS             605..2746
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995666.1"
                     /locus_tag="B1H29_RS03190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_03200"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_079160687.1"
                     /transl_table=11
                     /translation="MSDDRPPSPAPPAARESGGQPVLSLALASMMEEVHAHSGAVYLLG
                     ADPPLLEMAVMAGLPRAFAAPWERVALHAPIPVADAVRERRLVWVGDGEEMARRYPRVA
                     VVLPYPFALVALPVATESTTYGAVFLTWPGTRPAELSDREREQLTAACARLALRLERAT
                     AASLPIQPEPDLLAAPVVGGVPGTLGSLEAVRMVGRLPYGLCSLDLHGRVGFANPAAAE
                     LMDIPVSRLLGTHLWTSVPWLNDPVYEDRYRAALLSQQTTSFVALRPPGDWLSFRLYPT
                     RTGLSVRISRARGVSRPGPAEPVPDEAAPPRLVTISQVVSLTGALSEAVGVRDVVQLIA
                     DEIAPTVGSQGLIVLASRAGRLHVLGHRGYPDPFTAERFDGTPLTERIPSAQVLRTGVP
                     AFFESWDQLERLYPARRGTHDGFAAWAFLPLIASGRTVGTCVLAYERPHSFTTEERAIL
                     TSLAGLVAQALDRALIYDAKHRLAHGLQEALLPHSLPELSGIEAAARYLPATRGMDIGG
                     DFYDLVPARGGAAAVIGDVQGHNVTAAGLMGQVRTAVRAYTAVGQTPEEVVRSTNRLLI
                     DLGTELFASALYLRLDPGRGRAVLARAGHPPPLLRRPDGRVGVLEVPGGPLLGIDPSAE
                     YPRTEVALAPGSLLVLYTDGLVESPDVDFEDALADLAHRLSEAGDLALDELADHLVRHE
                     ADAGERLDDIALMLLRARD"
     gene            complement(3066..4433)
                     /locus_tag="B1H29_RS03195"
                     /old_locus_tag="B1H29_03205"
     CDS             complement(3066..4433)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978109.1"
                     /locus_tag="B1H29_RS03195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_03205"
                     /product="S1 family peptidase"
                     /protein_id="WP_055421219.1"
                     /transl_table=11
                     /translation="MSHKRIPKRRAAMAAGGVVALGAAAILLPNANASQDGGSSDDAAA
                     APRTLKAGDASDLASQLAGLLGDAFAGSYYDAGEQQLVVNVVPGDNNQVIVQAKKAGAE
                     VREVDNSMGELRTGARTLKSEATIPGTSWAIDPRTNKIRVTADSTVTGAEWDRLESTVD
                     SLGSGMATITKSTGTFKTFVSGGDAIFGGGARCSLGFNVTAGDGSPAFLTAGHCGVADD
                     QWSDAQGGQPIATVDQAVFPGEGDFALVRYDDPATEAPSEVNLGNQTIQISQAAEATVG
                     QQVFRMGSTTGLADGQVLGLDATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTQDGLAIG
                     LTSGGSGDCAVGGETFFQPVTTALEAVGATLGGDAAGGAGDGAGGEEAGAGDQAGGEEA
                     GAGDQAGGEEAGGGDAVGGGDEPGGEQDGAGGAGTGAGGAGDGTGVGGGDGSGLTG"
     misc_feature    4535..4548
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.03"
                     /tool="antismash"
     misc_feature    4658..4674
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.15"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="B1H29_RS03200"
                     /old_locus_tag="B1H29_03210"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="B1H29_RS03200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_03210"
                     /product="hypothetical protein"
                     /protein_id="WP_055421218.1"
                     /sec_met_domain="strepbact (E-value: 1.8e-37, bitscore:
                     126.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEISDADLDNVSGGLSVNAVGTVTGLVDGIAPVSGL
                     VNTAVGTVEGVTGLNTAPVTGLVAGL"
     gene            5329..6135
                     /locus_tag="B1H29_RS03205"
                     /old_locus_tag="B1H29_03215"
     CDS             5329..6135
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="B1H29_RS03205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_03215"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_055421217.1"
                     /transl_table=11
                     /translation="MQFRQQALTKLQSPEELDLPVRFARPQGWLVLSVTVVAMAAASVW
                     AVSGSVASTVGAPAILTHGQGSYLLQSPVSGQVTAVLARQGERLPADAPVLKVRTSEGE
                     TVVRTVDAGRVSALAATVGQIIQTGANVAAVEKVAHADDPLYATVYVPAENAAAIPAHA
                     SVDLTVQSVPTQQYGVLHGEVKSVDRSVQSAQTIAAFLGDSQLGEQFTKEGRPVAVTVR
                     LDTSKSTKSGYDWSSADGPPFELTSMTMAQGSIRLADQRPVDWLLP"
     misc_feature    complement(5351..5360)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.21"
                     /tool="antismash"
     gene            6132..8354
                     /locus_tag="B1H29_RS03210"
                     /old_locus_tag="B1H29_03220"
     CDS             6132..8354
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="B1H29_RS03210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_03220"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_055421216.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.5e-38, bitscore:
                     130.3, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQETRGRRRAAPPKRRVPGSRTKSVRTPTVLQMEAVECGAAS
                     LAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARGYGFTAKGMQMDLAALAEVTA
                     PAILFWEFNHYVVYDGMGRRFGRRGVFVNDPGKGRRFVSLEEFDGSFTGVVLTMEPGED
                     FTRGGRRPGVLGAMPARLRGTAGTLPAALLASLLLVAVGAAVPALSRTYIDMFLIGGQT
                     SLLGVLFASMAACVLLTIVLTWLQQANLLHGRIISSTLSSARFLRHLLRLPVTFFSQRS
                     PADLVQRLQSNDAVAETLARDLAAAGVDAIVVVLYAVLLYTYDPQLTFVGIGVALLNIL
                     AMRIVIRLRATRTAKLRADTARLTNTAYTGLQLIETMKATGGEDGYFRKWAGQHATTLE
                     EQQRLGVPSAWLGVVAPTLATLNSGLILWIGGMRAVEGHISVGLLVAFQALVVRFTAPL
                     TRLNGVAGRIQDFAADVARLKDVENFRADPLYDRPAGIDSTRRLHGHVELQNVSFGYNP
                     LDKPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYAPWDGVIRIDGQRLEDIPR
                     GALASSVSFVDQDVFLFEGSVRDNVALWDPSVPDDAVVDALRDAALYDVVMRRPGGVHS
                     RVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELVVMDNLRRRGCACV
                     VIAHRLSTVRDSDEIVVLQHGTVVERGRHEELVAHGGAYAALVRER"
ORIGIN
        1 cggcggcgag ggagaaaccg ctcgacggca cgcggcgcga ctcggccgat ccggcgaact
       61 cctcgggccc gagccacacc ggcctcccgc cccggtgggc gtcggcgacc ggggaaccgc
      121 cggcggcggg atagctgtcg cgcacgccgt acagggttcg cggcacgccg accgattcgg
      181 ccaggcagag cagctctcgg ttctcgccgg gtgtgtagag ggcggtgaag gaggcacccg
      241 cgaagaccag cgcctgttcg aggacgcggc gcagccgctc gtccgggtcc gggccggacg
      301 cgatcgccct cagggcaccc ttggcacgcg gcgttctcga ttcgcgttcc gcagcgccct
      361 cactgaccac gccgccatta cagcgcttat gggacacctg cgcagcccct gcgggcgggg
      421 aactcgacga ccagaggcct ggaaggggct cgcggacacg gtacggaccc cggtcggcgg
      481 tacaacgcgc tccacccccg cgggaacgtg ccgatgtgac gctcgccgtg cgcggtgggc
      541 ccacggggag gagatttgaa ccggggcccg gcgcaccgcg cgaccgtacg aggaggtggt
      601 cggcgtgtcc gacgatcggc cgccgtcacc ggcgcccccc gccgcgaggg aatcgggcgg
      661 ccaaccggtg ctgtcgctgg cgctggcctc gatgatggag gaggtccacg cgcactccgg
      721 ggccgtgtac ctgctcggcg cggatccccc gttgctggag atggcggtga tggcggggct
      781 gccgagggcg ttcgcggcgc cctgggaacg ggtcgccctg cacgcgccga tcccggtggc
      841 ggacgccgta cgtgagcgac ggctggtctg ggtcggcgac ggggaggaga tggcccggcg
      901 ctaccctcgc gtcgccgtgg tcctgcccta cccgttcgcc ctggtcgcgc tgcccgtcgc
      961 gaccgagtcc acgacgtacg gcgcggtctt cctgacctgg cccggtaccc gccccgcgga
     1021 gctgtccgac cgtgagcgcg agcagttgac ggcggcctgc gcacggctcg cgctccgcct
     1081 ggagcgggcg accgcggcga gcctgcccat acaacccgaa cccgatctgc tggccgcccc
     1141 ggtggtgggg ggcgtgccgg gcactctcgg ttcactggag gccgtgcgga tggtgggccg
     1201 cctgccctac ggcctgtgct ccctggacct gcacggccgg gtcggcttcg ccaatccggc
     1261 ggctgccgag ctcatggaca taccggtcag caggctgctg ggcacccacc tgtggacgtc
     1321 cgtgccgtgg ctgaacgacc cggtctacga agaccggtac cgggccgcgc tgctcagcca
     1381 gcagacgacc tccttcgtcg cgctgcgccc gcccggcgac tggctgtcgt tccggctgta
     1441 cccgaccagg accgggctca gcgtccgcat cagccgggcg cggggcgtgt cgcggccggg
     1501 tccggccgag ccggtgccgg acgaggccgc gccgccccgg ctggtgacca tctcgcaggt
     1561 ggtcagtctg acgggcgcgc tgtccgaggc ggtgggcgtg cgggacgtgg tgcagttgat
     1621 cgcggacgag atcgccccga ccgtgggcag ccagggtctg atcgtcctcg cctcgcgggc
     1681 ggggcggctg cacgtcctgg ggcaccgggg atatccggac ccgttcaccg cggaacgctt
     1741 cgacggcacg ccgctcaccg agcggatccc gagcgcgcag gtcctgcgga cgggggtgcc
     1801 cgccttcttc gagtcctggg accaactgga gcggctgtac ccggcgcgac gtggtacgca
     1861 cgacggcttc gcggcctggg ccttcctgcc gctgatcgcc tcgggcagga cggtcggcac
     1921 ctgcgtgctc gcctacgagc ggccgcactc cttcacgacg gaggagcggg cgatcctgac
     1981 cagcctggcc gggctggtcg cgcaggccct ggaccgggcc ctgatctacg acgcgaaaca
     2041 ccggctggca cacggactgc aggaggccct gctgccgcac tcgctgcccg agttgtccgg
     2101 catcgaggcc gccgcgcgct atctgcccgc cacccggggc atggacatcg gcggagactt
     2161 ctacgacctg gtaccggccc ggggcggggc ggccgcggtg atcggggacg tccagggcca
     2221 caacgtgacg gcggccgggc tcatgggaca ggtccgcacc gcggtccgcg cgtacacggc
     2281 cgtcggccag acgcccgagg aggtggtgcg cagcaccaac cggctgctga tcgacctggg
     2341 caccgaactg ttcgccagcg cgctgtacct gcggctcgac ccggggcgcg ggcgggcggt
     2401 gctggcccgc gcgggtcacc cgccgcctct gctgcgccgt ccggacgggc gggtgggggt
     2461 gctggaggtt cccggcgggc ccctgctggg catcgacccc tcggccgagt atccccgcac
     2521 ggaggtcgcc ctcgcccccg gctccctgct cgtcctctac accgacggcc tggtggagtc
     2581 ccccgacgtc gatttcgagg acgccctcgc cgacctggca caccggctgt ccgaggccgg
     2641 ggacctggct ctggacgagc tcgccgacca cctggtgcgg cacgaggcgg acgcgggaga
     2701 acgcctcgac gacatcgcac tgatgctgct gcgagctcgg gactgacgcg gggcgcccgg
     2761 ccgacgggac gggggcgtgg gccgggggcc gggcgcggtc ggaggcagac ggggcgagcg
     2821 ggggcgacgg cccgggaccg cggtgcggag ccgaggtgta cggcgatgca cagcccgggc
     2881 acggaaccgc ggcgcggagc ccccgcgtac gccccctcgc gtacggcgca ggcccccgct
     2941 gcggagagtg cggcggtccg gtcctcccgc cggagggccg gaccgccgac cgccggtcgg
     3001 ccccgtgggg ggccggccgg tggtttccgc gccggtgcgg aaggtacggg gagggagttc
     3061 cggggtcagc cggtcaggcc ggagccgtcc ccgccgccca cgcccgtgcc gtcaccggca
     3121 cccccggcac cggtaccggc tccgccggcg ccgtcctgct cgccgcccgg ctcgtcaccc
     3181 ccgccgacgg cgtcgccgcc cccggcctct tctccgccgg cctggtcacc ggcgccggcc
     3241 tcctcgccgc ccgcctggtc gcccgcgccg gcctcctcgc cgccggcccc gtcgcccgcg
     3301 ccgcccgccg cgtcgccgcc cagggtggcg ccgacggcct ccagagccgt ggtgaccggc
     3361 tggaagaagg tctcgccgcc gaccgcgcag tcgccgctgc cgcccgaggt cagacctatc
     3421 gcgaggccgt cctgggtgaa gagggagccg ccgctgtcgc cgggttcggc gcacacgtcg
     3481 gtctggatga ggccggtgac cgtcccctcg gggtagttga cggtggcgtc caggccgagg
     3541 acctgcccgt cggccagtcc ggtcgtgctg cccatgcgga agacctgctg accgaccgtc
     3601 gcctccgcgg cctgactgat ctggatggtc tggttcccga ggttgacctc gctcggcgcc
     3661 tcggtcgccg ggtcgtcgta cctgacgagc gcgaagtcgc cctcgccggg gaagaccgcc
     3721 tggtcgacgg tggcgatcgg ctgcccgccc tgcgcgtcgg accactggtc gtccgcgacg
     3781 ccgcagtgac cggccgtcag gaaggcggga gagccgtcgc ccgcggtgac gttgaagccc
     3841 agtgagcagc gcgcgccgcc gccgaagatg gcgtcgccgc ccgacacgaa ggtcttgaag
     3901 gtgccggtgg acttcgtgat ggtcgccatg ccggagccga ggctgtcgac ggtggactcc
     3961 agccggtccc actcggcgcc ggtgacggtg ctgtcggcgg tcacccgaat cttgttggtg
     4021 cgcgggtcga tcgcccacga ggtgcccggg atggtcgcct cggacttcag cgtccgcgcg
     4081 ccggtccgca gctcgcccat gctgttgtcg acctcgcgga cctccgcgcc cgccttcttc
     4141 gcctgcacga tgacctggtt gttgtcaccg ggtacgacgt tgacgacgag ctgctgctcg
     4201 cctgcgtcgt agtaggagcc ggcgaaggcg tcgccgagca ggccggcgag ctgcgaggcg
     4261 agatccgagg cgtcccccgc cttcagtgtc ctcggcgccg ccgcggcgtc gtccgaggac
     4321 ccgccgtcct gcgaggcgtt ggcgttcggc agcaggatgg cggccgcgcc gagcgccacc
     4381 acaccgcctg ccgccatcgc ggccctgcgc ttcgggattc gcttgtgact caaccttctc
     4441 gacctcctgg gacggggtcc gtgccgccgg tccggcggct gtcggggggc gcctggatgg
     4501 ctgttggtac gcacgggccg gacgggccgt tcaatcggac cggatcaaca cactccgcgt
     4561 tcggtcggcg acgctctgtt ccgatcaccg agcggaatcc ctgcttcagt gaatctgcac
     4621 atcgaatgcc caacccggtc atcctcgttg ggggatctgc acaagtccgc agcgcgccgc
     4681 acgtctttgg ggcgggtgtg ccggattcgc ctgcgtacgg gcaaggggta gcacgggacg
     4741 atgtccaccc tcatgtgaag aagtcgccgc gaagacgtgc gcgcacctgc acggttggac
     4801 gcccggacct cgcaagttcc ctggtgggac ggggtgattg attggaagcg cggaccggcc
     4861 gcgtgctttg atccatcgca ccgcaggggc cgcgagggct ccggaaaggg gcgccgggca
     4921 cctgcggtcg cggccgtcat ctgtccccag agggggccgc tccccccttg tgaaatggct
     4981 atacgaaggg aagttccatc atgaactcca ccccccaggt tgagaccgtc gagatctccg
     5041 acgccgacct cgacaacgtc tccggcggtc tgtccgtgaa cgccgtcggt accgtcaccg
     5101 gcctggtgga cggcatcgcc ccggtctccg gcctggtcaa cacggccgtc ggcaccgtcg
     5161 agggtgtcac cggcctgaac acggccccgg tcaccggcct ggtcgccggt ctctgatcgc
     5221 gctcatgtgc cgctgagtcc cggagccatc caggttccgg ggcttatcgg ccgttcttcc
     5281 gcgggttctc ctccgcggct cctccgggaa atctgggata cgtccgacgt gcagttccgc
     5341 caacaggccc tcacaaaact ccagtcaccg gaagagctcg accttccggt gcgtttcgcc
     5401 cgcccgcagg gctggctggt gctgtccgtg accgtggtcg cgatggccgc cgcgtccgtg
     5461 tgggcggtga gcggctcggt ggcctccacg gtcggcgcgc ccgccatcct cacgcacggc
     5521 cagggcagtt accttctgca gagcccggtc tccggccagg tcaccgccgt cctcgcgcgc
     5581 cagggcgagc ggctccccgc cgacgccccg gtgctgaagg tgcgtacgag cgagggcgag
     5641 acggtggtcc gcacggtcga cgccggccgg gtcagcgcgc tcgccgccac cgtcgggcag
     5701 atcatccaga ccggcgcgaa cgtcgccgcg gtcgagaagg tcgcccacgc ggacgacccg
     5761 ctctacgcca ccgtctacgt ccccgccgag aacgccgccg ccattcccgc gcacgcctcc
     5821 gtcgacctga ccgtccagtc ggtgccgacc cagcagtacg gcgtactgca cggggaggtg
     5881 aagtcggtgg accgctcggt gcagtcggcg cagacgatcg ccgccttcct cggcgacagc
     5941 cagctcggcg agcagttcac caaggagggc aggccggtgg ccgtgacggt ccggctggac
     6001 acctcgaagt ccacgaagag cggctacgac tggtcgtccg cggacgggcc gccgttcgag
     6061 ctgacctcca tgaccatggc ccagggttcc atccggctgg ccgaccagcg acccgtcgat
     6121 tggctgctgc cgtgagcacc gcacaggaga cccggggcag acgccgcgcc gccccgccca
     6181 agcgcagggt gcccgggagc cggacgaaga gcgtccgcac ccccaccgtc ctccagatgg
     6241 aggccgtgga gtgcggcgcc gcctccctcg cgatggtgct cggccactac ggcaggcacg
     6301 tcccgctgga ggagctgcgc atcgcctgcg gcgtctcccg cgacggctcg cgcgccagca
     6361 acctgctcaa ggccgcgcgc ggttacggct tcaccgccaa gggcatgcag atggacctgg
     6421 ccgccctcgc cgaggtgacg gcaccggcca tcttgttctg ggagttcaac cactacgtcg
     6481 tctacgacgg catggggcgc cgcttcggcc gccgcggggt gttcgtcaac gaccccggca
     6541 agggacgccg cttcgtgtcc ctggaggagt tcgacgggtc cttcaccggc gtcgtcctga
     6601 ccatggagcc cggcgaggac ttcacccggg gcggccgcag gcccggcgtg ctcggcgcga
     6661 tgccggcccg gctgcgcggc accgcgggca ccctgcccgc cgccttgctg gcgagcctgc
     6721 tgctggtggc ggtgggcgcg gcggtgcccg cgctcagccg cacctacatc gacatgttcc
     6781 tcatcggggg gcagacctcg ctgctcggcg tgctgttcgc gtcgatggcc gcctgcgtac
     6841 tgctcacgat cgtgctgacc tggctccaac aggcgaacct gctgcacggc cgcatcatct
     6901 cctccaccct ctccagcgcc cgcttcctgc gccatctgct gcggctgccg gtgaccttct
     6961 tctcccagcg cagcccggcc gacctggtgc aacgcctcca gtccaacgac gccgtcgccg
     7021 agacgctggc ccgtgacctc gcggcggcgg gcgtggacgc gatcgtcgtg gtcctgtacg
     7081 ccgtactcct gtacacgtac gacccccagc tcacgttcgt cgggatcggc gtggcgctgc
     7141 tcaacatcct cgcgatgcgg atcgtcatcc ggctgcgcgc gacccgcacg gcgaagctgc
     7201 gggcggacac ggcccggctg acgaacaccg cgtacaccgg cctccagctc atcgagacga
     7261 tgaaggcgac cggcggcgag gacggctact tccgcaagtg ggcggggcag cacgccacga
     7321 ccctggagga gcagcagcgg ctcggggtgc cgagcgcctg gctgggcgtg gtcgcgccga
     7381 ccctcgcgac gctcaacagc gggctgatcc tgtggatcgg cggcatgcgc gcggtcgagg
     7441 gacacatctc ggtcggtctg ctggtcgcct tccaggcgct ggtggtccgc ttcacggccc
     7501 cgctgacccg gctgaacggt gtcgcgggcc gcatccagga cttcgcggcc gacgtcgcgc
     7561 ggctcaagga cgtggagaac ttccgggccg acccgctcta cgaccgtccc gccgggatcg
     7621 actccacccg ccggttgcac ggccacgtcg agctgcagaa cgtctccttc ggctacaacc
     7681 cgctggacaa gcccctgctc accggcttcg acctgaccgt cggcccgggg cagcaggtgg
     7741 ccctggtcgg cggctcgggc agcggcaagt ccacggtgtc ccggctgatc tccgggctgt
     7801 acgccccttg ggacggcgtg atccgcatcg acgggcagcg tctggaggac atcccgcgtg
     7861 gggcgctggc gtcctccgtc tccttcgtcg accaggacgt gttcctcttc gagggctccg
     7921 tacgcgacaa cgtggcgctg tgggacccgt cggtcccgga cgacgcggtg gtggacgccc
     7981 tgcgcgacgc cgcgctgtac gacgtggtga tgcgccgccc gggcggcgtc cacagccggg
     8041 tcgagcagga cggccgcaac ttctccggcg ggcagcgcca acgcctggag atcgcgcggg
     8101 ccctggtgcg ccggcccagc atcctcgtcc tcgacgaggt gaccagcgcc ctggacgcgg
     8161 agaccgaact ggtggtcatg gacaacctgc gccggcgcgg ctgcgcctgt gtggtgatcg
     8221 cgcaccggct cagcaccgtg cgggacagcg acgagatcgt ggtgctccag cacggcacgg
     8281 tcgtggagcg gggccggcac gaggaactgg tcgcgcacgg cggcgcctac gcggcgctgg
     8341 tcagggagcg ttgagatgag agccgtacac gaaggacagg gcgacctcgt cctcggggcc
     8401 ctcggctcgc tgggcacccg tgtcgactgc gccgggttcg gccgcctgga cctggagggt
     8461 ccgcaggtgc tgtggctggt ggcgtccggc gcggtggacc tgttcgcggt cgacgccggg
     8521 gaacagggcc actggcacca tctgggccgc ctggaggcgg gctcggtcct gctcggcccg
     8581 gtcaccgggc cccagcacac gctggtcgcg cgtccgctgc gggactgcgt ggtgcaccgc
     8641 atcggactgc gcgagctgta ccagccggcg cacacccaga cctggtcgta cgacgagtac
     8701 ggcacccccc agtacgtacc gcccacgacg agcccgctgg agtacgccct cgccctcggg
     8761 gtgggccgca gcctgaccgt gctcttccag gcgccgatgg ccaccgagcg ggccgcggca
     8821 cccacggacg acgacgtgtt ctggatgcag gtcccgccgg gcagcgtgca gtacggggcg
     8881 gtgtacggcg aggaggcggc cgccgacctg ctgatggacc cgtcgctgtg gcagagcatg
     8941 gtcgaccagc agtaccggct gctgaccacg ctggaccgct ggatcgagca gctcgagcgc
     9001 acccacgaga cccgtacggc ggccggtatc aaggccggtg aggcggtgcg cgcccaggcc
     9061 gaccggacgc tgctggcgtc catcggcaag cggtcggggc ggcgcgccac ggccgccgac
     9121 gccgacgcca cgtacgcggc ctgcgaactg gtcgcccgcg ccgccggcat cccgctcgcc
     9181 gagcccgccc gaagcggcac ggagagcgac cggctcgacc cggtggagcg gatcgccgtc
     9241 gcctcgcgcg tgcgcacccg gaccgtgcgg ctggagggcc gctggtggcg ggacgacgtc
     9301 gggccgctgg tcggccaccg ggccctgtcg ggcgcgccgg tggcgctgct gtggcggcgc
     9361 ggcggctatg cggccgtcca tccggcgacc ggccgggaga cgccggtcga gagggcgaac
     9421 gccgaggagt tcgagccgcg tgcggtgatg ttctaccggc cgctgcccga acgccggctc
     9481 agtccgctgc ggctgctgcg cttctgtatg gcgggcacgc gcggtgatct gaccgggctg
     9541 ctgctcgccg gactggtgac cgtggcgatc ggcgcgctgg tgccgatcgc caccggcaag
     9601 gtgctcggcg agttcgtgcc gaaggcgcag accgagctga tcgtgcaggt ctgtctcgcg
     9661 gtgatgctga gcagtgtcgt cgcggcggcc ttcatgctgc tgcagaacct caccatcctg
     9721 cggctggagg gccgtgtcga ggcgaccctg cagcccgcgg tgtgggaccg gctgctgcgg
     9781 ttgccgacga ggttcttcac cgagcgttcc accggtgagc tggcgagcgc cgccatgggc
     9841 atcagcgcga tccgccggct gctggcgggc gtcggtccga cggtggccca gtcggtgacc
     9901 gtcggggcga tgaacctggg cctgctgctc tggtacagcg tgccgatggc actcgcggcg
     9961 atcggcatgc tggtggtcgt cgccgccgtg ttcctggcgc tcggcctgtg gcaggtgcgc
    10021 tggcagcggc ggctggtcaa gctcaccaac aagctgaaca accaggcctt ccagaccctg
    10081 cgcgggctgc ccaagctgcg cgtcgccgcg gccgagaact acgcgtacgc ggcgtgggcg
    10141 gagcagttcg cgcgcagccg tgagctccag cagcaggcgg gacgcgtcaa gaacctgaac
    10201 gcggtgctcg gcgcgg
//
