LOCUS       NZ_CP019724            10990 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Streptomyces pactum strain ACT12 chromosome, complete genome.
ACCESSION   NZ_CP019724
VERSION     NZ_CP019724.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces pactum
  ORGANISM  Streptomyces pactum
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Streptomyces pactum ACT12 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8550793)
  AUTHORS   Xue,Q., Yan,X., Jia,L. and Yan,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2017) College of Life Sciences, Northwest A&F
            Unversity, No.22 Xinong Road, YangLing, Shaanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2016
            Assembly Method                   :: SMRT v. 2.3.0
            Assembly Name :: version 2.3.0 of the PacBio Single Molecule Real
            Time
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002005225.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:58:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,315
            CDSs (total)                      :: 7,221
            Genes (coding)                    :: 6,992
            CDSs (with protein)               :: 6,992
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 84 of 229
            Pseudo Genes (incomplete)         :: 195 of 229
            Pseudo Genes (internal stop)      :: 15 of 229
            Pseudo Genes (multiple problems)  :: 62 of 229
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:20:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 231235
            Orig. end                         :: 242225
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019724.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Northwest A&F Unversity.
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10990
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10990
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10990
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5990](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5990
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(573..1400)
                     /locus_tag="B1H29_RS00875"
                     /old_locus_tag="B1H29_00900"
     CDS             complement(573..1400)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387424.1"
                     /locus_tag="B1H29_RS00875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_00900"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_055422193.1"
                     /transl_table=11
                     /translation="MDIEVLGALSVREHGTSITPTAPKPRQVLALLALQADRVVQVSSL
                     IEELWDDEPPRSARTTLQTYVLQLRELIGEALAKSGEEDVTAKDILVTLPGGYRLDTRG
                     GRVDFREFEQQAGAGYRAMDAEDFTSAARRLREALSLWTGSALADVTAGSRIGLEVKRL
                     EETRLCALDQRIEADLRLGRHRELLSELTVLVNRHQMHESLHGQFMVALHRSGRRGEAL
                     SVYQRLRTTLVRELGLEPSAMMSRLQRSILTAAPETAGTRSPEATGRTVARVG"
     misc_feature    1396..1411
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(1509..1518)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    complement(1761..1775)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.38"
                     /tool="antismash"
     gene            complement(1859..2476)
                     /locus_tag="B1H29_RS00880"
                     /old_locus_tag="B1H29_00905"
     CDS             complement(1859..2476)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133214.1"
                     /locus_tag="B1H29_RS00880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_00905"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_055422192.1"
                     /transl_table=11
                     /translation="MVKQERARRTREALIKSAATVFDRDGFSVASLTAISSLAGVSNGA
                     LHFHFASKAALADSVEEAAAQRLERIVTAPEGAAGNALQMLVDTSHELARRLTEDVVLR
                     AGFELSRDQVRRRGPGLYRQWHRWVEGALKRAAGEGVLNQVTPEDAAASVVAATTGFEV
                     LGPQDPQWLSHQTVTRFWQLLLPRLAEASALGGLVAAGSRPR"
     gene            complement(2490..4082)
                     /locus_tag="B1H29_RS00885"
                     /old_locus_tag="B1H29_00910"
     CDS             complement(2490..4082)
                     /EC_number="6.-.-.-"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432460.1"
                     /locus_tag="B1H29_RS00885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_00910"
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="WP_055422191.1"
                     /transl_table=11
                     /translation="MTVLNDVAQVSARLSGGRGCVAELRGIRARALAGPGGEATAAQHA
                     RGKLTARERIGLLLDPESFCEVGQLRRHRATGFGLEAKRPYTDGVVTGWGTVEGRTVFV
                     YAHDFRIFGGALGEAHAAKIHKIMDMAIAAGAPLVSLNDGAGARIQEGVCALAGYGGIF
                     QRNTRASGVIPQISVMLGPCAGGAAYSPALTDFVFMVRDTSQMFITGPDVVRAVTGEEV
                     THNGLGGAGVHAETSGVCHFAYDDEETCLAEVRYLLSLLPPNNRQFPPRVRCADPHTRR
                     SKVLADLVPADGSRPYDMARVVEELVDDGEYLEVHERWARNIICALARLDGQVVGIVAN
                     QPQVLAGVLDIGASEKAARFVQMCDAFNIALITLLDVPGFLPGVDQEHGGIIRHGAKLL
                     YAYCNASVPRISLVLRKAYGGAYIVMDSQSIGADLTYAWPANEIAVMGAEGAADVVFRR
                     QIADAEDPEAMRARMVKEYRAELMHPYYAAERGLVDDVIDPAETREVLVRSLAMLQAKN
                     ADLPSRKHGNPPQ"
     misc_feature    complement(4115..4132)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.64"
                     /tool="antismash"
     gene            complement(4483..4701)
                     /locus_tag="B1H29_RS00895"
                     /old_locus_tag="B1H29_00915"
     CDS             complement(4483..4701)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004994460.1"
                     /locus_tag="B1H29_RS00895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_00915"
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="WP_055422190.1"
                     /transl_table=11
                     /translation="MGEPGVMGPALRIERGRADADELAALTVVLCSVVAGRAADGAPEP
                     PAPAPAPAPWRRADRTGSAYRSPYCWR"
     gene            5001..5990
                     /locus_tag="B1H29_RS00900"
                     /old_locus_tag="B1H29_00920"
     CDS             5001..5990
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387457.1"
                     /locus_tag="B1H29_RS00900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_00920"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_055422189.1"
                     /sec_met_domain="AfsA (E-value: 2.4e-25, bitscore: 87.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTASTFRFEGPSIEQAPAGTALPASRAALRHGPLTTTVPRELVHR
                     AAVAEVMLTDWKRLDDTHFAMAAQWPRSHSFFTPVGVHHDPLIAAETIRQIGALLAHAE
                     FGVPFGHQFLMEELTLTVRPEQVRTGRAPAALDLDVTCTEVRTRGRRLSGLRYETTILR
                     EGRPAATGQISFTALAPAVYRRLRPEHVFSAGHRPIPLTAPVAPQSVGRLSPADVVLSP
                     TGDPDRWQLRVDTRHPVLFDHQVDHVPGMVLLEAARQAATAVLHRPSLLPAGLTCAFTK
                     YAELDLPCLIDALHLPRTAPGDPDTVLVTGHQDGHPVFSATVTAAPQD"
     gene            complement(6601..7473)
                     /locus_tag="B1H29_RS00910"
                     /old_locus_tag="B1H29_00925"
     CDS             complement(6601..7473)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387428.1"
                     /locus_tag="B1H29_RS00910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_00925"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_055422406.1"
                     /transl_table=11
                     /translation="MILVTGATGTVGGEVLRRLPAHLAVRVMARDPSRVARPGCRAEVV
                     AGDYGDPCSLGRAVEGVGRALVLTSRVGGDDDARFVDAARAAGVRHLVKVSAAAVADSG
                     ADDVLTRWQRRNEDLLLGSGLRWTVLRPRSFMSNTLSWAVSIAGEQVVRGLYGTSLNAC
                     VDPRDVAAAAVCALTEDGHEGRIHTLTGPEPISAVEQTAQLGRLLGRPLRFEELSPRRA
                     RVLLRERYPEPVVEALLAGAERQRAGAKAGVEDTVGRLTGRAARSFRTWAGDHLGAFGA
                     VPGAAGAGR"
     gene            7635..8303
                     /locus_tag="B1H29_RS00915"
                     /old_locus_tag="B1H29_00930"
     CDS             7635..8303
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387458.1"
                     /locus_tag="B1H29_RS00915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_00930"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_079159927.1"
                     /transl_table=11
                     /translation="MVKQDRAIRTRRNILEAAAKIFEERGYQAATIAEILSTAGVTKGA
                     LYFHFESKEQLAQGVLHEQDQRFVIPDRACKVQELADVVLLHAYRLQSDPMVRAGVRLT
                     MDQHAEGLDRSGPFLRWSTVCRQLLEQAQAQGELLPHVIPSVTADIMVSTFAGVQSMSQ
                     TTTDYQNLRFQTSALLRHLLPSIAVPSVLASLDLTESRGETVHEEIRHQLQPQPATAVR
                     "
     gene            complement(8368..9900)
                     /locus_tag="B1H29_RS00920"
                     /old_locus_tag="B1H29_00935"
     CDS             complement(8368..9900)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /locus_tag="B1H29_RS00920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B1H29_00935"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_079159928.1"
                     /transl_table=11
                     /translation="MGRCLDADVVVVGGGPVGLLVAAELGERGVRTVVLEAGVCVSRGP
                     RARVLHARAVQGLARRGYVGELGRWVDAGGTGVEVGFRFGGVEGLSIVAPVSEPGPVLR
                     CPQAELECVFEGRARGVGVRVLRGVRVSGVVQDGRGVRVVGWGAGGRRVECRAGFLVAA
                     DGGRGTVGGLVGVVSESFGATASGLSAVVGREVVRDLPAGWHRTPRGWIVAHEIAGVGL
                     HVKTLDCSGPHPGRQVPPSVGELRGEVARIAGREIELGRVRWLSRFSDFSRLACSFRWG
                     RVFLVGDAAHLCFPVGAQGLSVGVQDALNLGWKLALAVRGRAGVGLLDSYDLERRPAAR
                     RVIGSTRVQLALMRPGCGAESLRAVFAGLVAADGGSRFLSDLVSGQDTVLPGRTRRPSV
                     WEGRFLQNVGLGVGGGCVDVIGLLRPGRPLLLLLGAGGGRYARGARGWADVVRVVRAGP
                     VAEVGCEALLVRPDGYIAWAAAPGGDCLEDALALYFGGRLRGPGSGGRVRLR"
     misc_feature    complement(9937..9946)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     gene            10099..10898
                     /locus_tag="B1H29_RS36985"
     CDS             join(10099..10437,10440..10898)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558177.1"
                     /locus_tag="B1H29_RS36985"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS5 family transposase"
                     /protein_id="WP_234393192.1"
                     /ribosomal_slippage=""
                     /transl_table=11
                     /translation="MLSGLVPDELWALFRRVVPPAEVKRPQGGGRRRSGDREALAAIIF
                     VATSGCTWRQLPPVFGPAWQTVYRRFAQWSRDPVWARPYRVVLDELGARGELAWSRCAI
                     DSVSIRAVKGMLTGPNPTDRGKSGTNIHLLTDRNGLPLSLGISGANMHDSQALEPLVRG
                     IPPIRSRRGPRRRLPAKLHADKDYDYKHLRRWLRSRGIPHRIARKGIEPSQRLGRHRWV
                     VERTVSWLGGCRRLHRRYERKPEHFLAFVGIAAALICHRRLTT"
ORIGIN
        1 tgcgcgacca cacctgccgc ataccggcgg ggccgccgcg cagcggccga cgcgagcggt
       61 ggcgcgcctg ctgcccgggg tccgccggcc gggcggggcc cgtcctgccc gccgtgctcg
      121 tcccggtcac cggtggcgaa gtccgtcgcc ccggaccgcg aggccggcac cgtcccggtc
      181 gaggccggcc aggcctgcgg ccgcctgccg caccgggacg cgcgcggtgc gggtcgcgcg
      241 gctgccgccg ccccggcacg gccggccggc cgggtgaagg cgtagcggcc gggaaagccg
      301 atgcggtggt accggaacgg gggcagccgg gcgggcaggc cgccggcggc gagctgcgca
      361 gagggcctca cggccgcttg gcacctgcgc gggcatcccg ggtacacgag cccgcccgcc
      421 cccgggccgg gggcacggcc ggcacggccc ggcggccggc cgcgggcgcg gggccggccg
      481 cggacgcggg gccgggcgcg gtcccggcag ccgggcgcgg acgcgggcgc ggcggccggg
      541 cgcggacgcg gcggccgggc gcgggggcgg ggtcagccca cccgggccac ggtccggccg
      601 gtggcctccg ggctgcgggt cccggcggtc tccggggcgg ccgtcaggat ggagcgctgc
      661 agccggctca tcatcgccga cggctccagg cccagctcgc gtacgagggt ggtgcgcagc
      721 cgctggtaga cgctgagcgc ctcgccgcgc cggccggagc ggtgcagggc caccatgaac
      781 tggccgtgca ggctctcgtg catctggtgc cggttgacga ggacggtcag ttcggacagc
      841 agctcgcggt ggcggccgag ccgcagatcg gcctcgatgc gctggtccag cgcgcacagc
      901 cgggtctcct ccaggcgctt gacctcgagg ccgatgcggc tgccggcggt cacgtcggcg
      961 agcgcggagc cggtccacag cgacagcgcc tcccgcagcc gccgtgcggc gctggtgaag
     1021 tcctcggcgt ccatggcccg gtagcccgct ccggcctgct gctcgaactc acggaaatcg
     1081 acccggccgc cccgggtgtc caggcggtag ccgccgggga gggtgaccag gatgtccttg
     1141 gcggtgacgt cttcctcgcc ggacttggcc agcgcctcgc cgatgagctc ccgcaactgg
     1201 agcacgtagg tctgcagggt ggtgcgtgca ctgcgcggcg gctcgtcgtc ccacaactcc
     1261 tcgatcaggg aagagacctg gaccacgcgg tcggcctgca gcgcgagcag tgccagcacc
     1321 tgccgcggct tcggggccgt cggtgtgatc gaggtgccgt gctcgcgtac tgacagggcg
     1381 cccagaactt cgatgtccac tgtcttttcc ccctttttgt tccatgccgg gttgcatacg
     1441 gatccctacc gcgagggccg tcgctggtgc gcctggacgg cgctcgacga ctccggctag
     1501 aacacgatta aaaaaacagc acagtcggtt tgtcaatggg gaggagatgt gcagactcgc
     1561 acgcgttgcc ccgccgctgg tggcggcctc gtccggacga gggcggcggg aggcccggaa
     1621 ccccggcgga tgggcgccgt tgacccccgg gcggccgggg tgcgggtgcg gcggcgggcc
     1681 ggcgcacgga gcggggcggc gtgcggggag ccgtgcggcc gggcgccgcc gtggtgcccg
     1741 cacgctcaag taggtggaga caaaaaggat aatcaacccg cttatcagac cgcgtggtct
     1801 gttatgctct cccttcgggc cgtctcccct ctgcgggtgc cggtgtggcc tgtcgtcctc
     1861 accgggggcg gctgccggcc gccacgagac cgccgagggc ggaggcctcg gccagccgcg
     1921 gcagcagcag ttgccagaac ctggtgaccg tctggtgcga gagccattgc gggtcctgcg
     1981 ggcccagcac ctcgaagccg gtggtcgccg ccaccacgga ggccgccgcg tcctcgggcg
     2041 tgacctggtt gagcacgccc tcgccggcgg cgcgcttcag cgccccctcg acccaccggt
     2101 gccactggcg gtacaggccg ggcccccttc ggcggacctg gtcccggctc agctcgaacc
     2161 cggcgcgcag caccacgtcc tcggtgagcc ggcgggccag ctcgtgcgag gtgtccacca
     2221 gcatctgcag ggcgttgccg gccgcgccct ccggggccgt gacgatgcgc tccagccgct
     2281 gcgccgcggc ctcttccacc gagtcggcca gagccgcctt gctcgcgaag tggaagtgga
     2341 gcgcgccgtt gctcactccg gccagcgagc tgatcgccgt gagagaggcc acgctgaagc
     2401 cgtcgcggtc gaagaccgtg gcggccgact tgatcagcgc ctcgcgggta cgccgcgctc
     2461 gctcctgttt gaccatgggg aatgctccgt tactgcggcg ggttgccgtg cttgcgggag
     2521 ggcaggtcgg cgttcttggc ctggagcatc gccagggatc tgacgagcac ctcgcgggtc
     2581 tcggcggggt cgatgacgtc gtcgaccaga ccgcgctcgg ccgcgtagta ggggtgcatc
     2641 agctcggccc tgtactcctt gaccatgcgc gcccgcatgg cttcggggtc ctcggcgtcg
     2701 gcgatctgac ggcggaagac gacgtcggcc gcgccctcgg cgcccatcac ggcgatctcg
     2761 ttggcgggcc aggcgtaggt gaggtcggcg ccgatggact ggctgtccat gacgatgtag
     2821 gcgcccccgt acgccttgcg caggacgagc gagatccggg gcacgctcgc gttgcagtag
     2881 gcgtagagca gcttggcgcc gtggcggatg atcccgccgt gctcctggtc cacacccggc
     2941 aggaacccgg gtacgtccag gagggtgatg agcgcgatgt tgaaggcgtc gcacatctgg
     3001 acgaaccgtg cggccttctc gctggccccg atgtccagga caccggccag gacctgcggc
     3061 tggttggcga cgatgccgac gacctggccg tccagccggg ccagggcgca gatgatgttg
     3121 cgcgcccagc gctcgtggac ctccaggtac tcgccgtcgt cgacgagttc ctcgacgacc
     3181 ctggccatgt cgtaggggcg gctgccgtcc gccggcacca ggtccgccag caccttcgaa
     3241 cggcgggtgt gcggatcggc acaccgcacg cgcggcggga actgccggtt gttcggcggg
     3301 aggagggaca ggaggtagcg gacctcggcg aggcaggtct cctcgtcgtc gtaggcgaag
     3361 tggcagacgc cggacgtctc ggcgtgcacg ccggcgccgc cgaggccgtt gtgggtgacc
     3421 tcctcgccgg tgacggccct gaccacgtcc gggccggtga tgaacatctg cgaggtgtcg
     3481 cggaccatga acacgaagtc cgtgagggcg ggactgtagg cggcgccgcc cgcgcacggg
     3541 ccgagcatca cgctgatctg cgggatgacc ccggacgccc gggtgttgcg ctggaagatg
     3601 ccgccgtacc cggccagcgc gcagacaccc tcctggatcc gcgcgccggc accgtcgttg
     3661 agggagacca gcggcgcacc cgccgcgatg gccatatcca tgatcttgtg gatcttcgcg
     3721 gcgtgggcct cgcccagcgc cccgccgaag atccggaagt cgtgcgcgta gacgaagacc
     3781 gtacggccct ccaccgtgcc ccagccggtg acgacaccgt cggtgtacgg ccttttggcc
     3841 tccaggccga acccggtggc ccggtgccgg cgcagctgcc cgacctcaca aaaggactcc
     3901 gggtccaaca gcagcccgat acgctcccgc gccgtcagct tgcccctggc atgctgcgcc
     3961 gccgtcgcct ccccacccgg cccggccagc gcccgcgccc ggatcccgcg cagttcggcc
     4021 acgcacccgc ggccgcccga cagacgcgcg gacacctgag cgacgtcatt gaggactgtc
     4081 acgctgcctc ctccctcctg aacgcggccg gcacggaacc ccaggctaac aaaccgtcta
     4141 tgcggtttat aagggggttg tgtggcccac gcatgaaaaa gccctggtaa gtgccgcccg
     4201 cccggccacc gcccgggcgg ccggcggccc ggtagccggt gacccggtag ccggtagccg
     4261 gtagccggtg gccggtagcc ggccggccgt tgggcggtgg ccggttggcc ggtggccggc
     4321 cgaccgttgg gcggtggccg gttggccggt ggcccggtag ccggtggccg gccgaccgtt
     4381 gggcggtggc ccggtagccg gtagccagtt gggcggttgc cggtggcccg gtggccggtg
     4441 gccggccgac cgttgggcgg tggccggttg ggcggtgggg cgttaccgcc agcagtacgg
     4501 cgagcggtag gccgagccgg tccggtccgc ccgccgccac ggcgcaggcg caggggcggg
     4561 ggcggggggc tccggtgcgc cgtccgccgc ccgcccggcc acaaccgagc acagcacgac
     4621 ggtcagcgcg gccagttcgt ccgcgtcggc ccggccgcgt tctatgcgca acgcgggccc
     4681 catgacgccg ggttcaccca tgtcggctcc tctccacccc ggccaccgtg cggcaccgca
     4741 ctacaaaggc gcctcagcac cgctcgagaa acgctcgggc ggtcggccgg aggcggcaca
     4801 gcggccctgc ggcggccccg tcagccatga gctggacaaa caccggagaa gggaccggcc
     4861 cggcagaact ctcccgtccc gcccccagga cgccccggga cgaacctgcg cggaagctgc
     4921 ggttcctctg gaaatcaaac cgggtggtct ctacttttat gccctctgca gcacatctcc
     4981 gatcccactc agggggagcg atgaccgcga gcacgttccg gttcgaaggt ccgtcgatcg
     5041 agcaggcgcc ggcgggaacc gccctgccgg cctcccgggc agcgctgcgt cacgggcctt
     5101 tgaccacgac ggtccccagg gaactggtgc accgcgcagc cgtcgccgag gtcatgctca
     5161 ccgactggaa gcgcctggac gacacccact tcgcgatggc cgcgcagtgg ccgcgcagcc
     5221 acagcttctt cacccccgtg ggagtccacc acgacccgct gatcgccgct gaaacgatcc
     5281 gtcagatcgg tgccctgctc gcccacgccg agttcggcgt ccccttcggc caccagttcc
     5341 tgatggagga actcaccctc acggtccgtc ccgagcaggt gcggaccggc cgggcacccg
     5401 ccgcgctgga cctcgacgtc acctgcacgg aggtcaggac acgcggccgc cggctgtccg
     5461 gcctgcggta cgagacgacg atcctgcgcg agggacggcc cgccgcgacg ggacagatct
     5521 ccttcaccgc cctcgctccc gccgtctacc gccggctgcg tccagagcac gtcttcagcg
     5581 ccggacaccg tccgatcccc ctgacggctc cggtggcgcc gcagagcgtg ggacgcctct
     5641 cacccgccga cgtggtgctc tcccccaccg gcgacccgga ccgctggcaa ctgcgagtgg
     5701 acacccggca cccggtcctc ttcgaccacc aggtcgacca cgttccgggc atggtgctgc
     5761 tcgaggccgc ccgccaggca gcgaccgcgg tcctgcacag accctccctc ctgccggccg
     5821 gcctcacatg cgcgttcacg aaatacgcgg agctggacct gccctgtctg atcgacgccc
     5881 tgcacctgcc ccgcaccgcc cccggcgacc cggacacagt cctggtcacc ggccaccagg
     5941 acggccaccc cgtgttcagc gcgaccgtga ccgccgcccc acaggactga ccgcacaccc
     6001 cacaacacac cgccacaccg caggacacac cacacgcacc cggggagcag cccccgccgc
     6061 ggcccgcggg acgggccagc ccatgaaacg ggacggcccc gagccacaac ggcgcaccgt
     6121 ccggcgcggc atgccgtccg gcggcggcgc accgtccggc ggcggcgcac cgtccggcgg
     6181 cggcgcaccg tccgacgcgg cgtaccgtcc gacgcggcgc accgtccgac gcggcgcacc
     6241 gtccggcgcg gcgtgccgtc cggcggcgtg ccgctgcccg gggcggcagg gccgcgcggc
     6301 gaccgcacct gacggccacg cacccgcacc cccttgcccg gcccgccaca ccccgtgaag
     6361 gctccgggca agccccccgc cgccccgccc ccgccacacc cggcacggca ccggcacgcc
     6421 cgcgggacgg ggcggccccg gtccccgacg gcgccccgtc cggcggcggc gcaccgtccg
     6481 gcgcggtgcg ccgccgcccg gggaagggcg gcggggaccg cggcgaccgg acctgacggc
     6541 cgccgtacca cccctacgcc ccctgagccc tcacaccccg tgaaggctcc gggcaagccc
     6601 tcaccgcccc gcccccgccg cacccggcac ggcaccgaac gccccgaggt gatccccggc
     6661 ccacgtgcga aaggaccggg ccgcgcgccc ggtcagccgc ccgaccgtgt cctcgactcc
     6721 cgccttggct ccggcccgct ggcgttccgc accggccagc aacgcctcga cgaccggctc
     6781 gggataccgc tcgcgcagca ggacacgcgc ccggcggggg gacaactcct cgaagcgcag
     6841 cgggcggccc agcagccggc cgagctgcgc cgtctgctcg accgcgctga tcggctccgg
     6901 tccggtcagc gtatggatcc gcccctcgtg cccgtcctcg gtcagcgcgc acacggctgc
     6961 ggcggcgacg tcgcgcgggt cgacgcacgc gttgagcgag gtgccgtaca gaccccgcac
     7021 cacctgctca ccggcgatgg acaccgccca ggacagcgtg ttggacatga aggaccgcgg
     7081 gcgcagcacg gtccaccgca gaccggaacc cagcaacagg tcctcgttcc tgcgctgcca
     7141 gcgggtgagg acatcgtccg ccccgctgtc cgcgacggcc gccgcggaca ccttgaccag
     7201 atgccgcacc cccgccgcac gcgcggcatc cacgaagcgc gcgtcgtcgt caccgccgac
     7261 acggctggtg aggaccagcg cgcggcccac cccctcgaca gcgcgcccca gcgagcacgg
     7321 atcgccatag tcaccggcca ccacctcggc ccggcaaccc ggacgcgcca cccgcgaggg
     7381 gtccctggcc atgacacgca ccgcgagatg cgcgggcagc cggcgcagga cctccccccc
     7441 gacggtcccg gtggcgcctg tcacgagaat caccgatgtc ctccctccgc cgcaccaggc
     7501 ccacacgccg cccgaacccg cgtcctgaga ccgcgcccgg caccggatgt aagatacaaa
     7561 ctatgcggtt tttttgtggg gtcggccggg cctccgcccg ccgaaaccaa cgccaggaaa
     7621 agggaggacc ccacgtggtg aaacaagacc gtgccatccg cacacgaagg aacatcctcg
     7681 aagcagcggc aaagatcttc gaggaacgcg gctaccaggc cgccaccatc gccgaaatcc
     7741 tcagcaccgc aggcgtgacc aagggagcgc tgtacttcca cttcgaatcg aaggaacagc
     7801 tcgcccaggg cgtcctgcac gaacaggacc agcggttcgt catccccgac cgcgcctgca
     7861 aggtccagga actcgccgac gtcgtcctgc tgcacgccta ccgcctgcag tccgacccga
     7921 tggtacgggc aggcgtgcgg ctgaccatgg accagcacgc cgaaggcctc gaccgcagcg
     7981 gccccttcct gcgctggagc accgtatgcc ggcaactgct ggaacaagca caagcccagg
     8041 gcgaactact gccccacgtc ataccatccg tcaccgccga catcatggtc agcacattcg
     8101 cgggcgtcca gtccatgtcc cagaccacca ccgactacca gaacctgcgc ttccagacct
     8161 ccgcactgct gcgccacctc ctccccagca tcgccgtgcc atccgtactc gcctccctgg
     8221 acctgaccga atcacgcggc gaaaccgtcc acgaggaaat ccggcaccaa ctccagcccc
     8281 agccggcaac cgccgtcaga taaacacccc ccccacccca ccccaccaac cccccaccaa
     8341 accccggcca ccacacccgc cccccggtca ccgcagccgc acccgccctc cagaccccgg
     8401 accccgcagc cgcccgccga aatacagcgc caacgcatcc tcaagacagt ccccacccgg
     8461 agcggcagcc caggcaatat aaccgtccgg ccgcaccagc agagcctcac acccaacctc
     8521 cgcaaccggc cccgcccgaa ccaccctcac cacatccgcc cacccacgcg ccccccgcgc
     8581 ataccggccg ccccccgcac ccagcaacag cagcaacggc ctacccggcc gcagcaaccc
     8641 gatcacatca acacaccccc cacccacccc cagccccaca ttctgcaaaa acctcccctc
     8701 ccacaccgaa ggccgccggg tacgccccgg aagaaccgtg tcctgaccac tgaccagatc
     8761 actgagaaaa cgactccccc catcagccgc caccagaccg gcgaacacag cacgcaacga
     8821 ctccgcccca caccccggac gcatcaacgc aagctgcacc cgcgtactgc caatcacccg
     8881 ccgagcagcc ggacgccgct ccaggtcata actgtcgagc aaccccacac ccgcccgacc
     8941 ccgcaccgcc aacgccaact tccaccccaa attcaacgca tcctgcaccc ccacactcaa
     9001 cccctgcgca cccaccggga aacacaagtg cgccgcatcc cccaccagaa aaaccctccc
     9061 ccaccgaaac gaacaagcca gccgcgaaaa atcactgaac cgactgagcc accgcacccg
     9121 ccccaactcg atctcccgcc ccgcgatccg agcaacctca ccacgcaact cccccaccga
     9181 cggcggcacc tgacgaccag gatgcgggcc cgaacagtca agcgtcttca catgcagacc
     9241 cacccccgca atctcgtgcg ccacgatcca cccacgcggc gtacggtgcc agcccgccgg
     9301 cagatcccgc accacctccc gccccaccac cgccgacaac ccggacgccg tagccccgaa
     9361 actctccgac accaccccca ccaacccacc caccgtgcca cgccccccgt ccgccgccac
     9421 caaaaaccca gcccgacact caacccgccg ccccccagcc ccccacccca ccacccgcac
     9481 cccacgcccg tcctgcacca ccccactcac ccgcacccca cgcaacaccc gcacccccac
     9541 accccgcgcc cgcccctcaa acacacactc caactccgcc tgcggacacc tcaacacagg
     9601 acccggctcc gaaaccggcg caacaatcga caacccctcc accccaccga accgaaaccc
     9661 cacctccacc cccgtcccac ccgcatccac ccaccgcccc aactcaccga catacccccg
     9721 cctcgccagc ccctgcaccg cccgcgcatg caacacccgc gccctcggcc cccgcgacac
     9781 acacaccccc gcctccaaca ccaccgtccg caccccccgc tcccccaact ccgccgccac
     9841 caacagaccc accggcccac cccctaccac caccacatcc gcatccagac aacgccccat
     9901 taccaacacc ccctccagcg cccgcccacc aggaccccaa aaaatatacc gaacagacgg
     9961 ttcttaacaa gacacccgac acccacacac cccccacaca tccaccaccc aaaacccaaa
    10021 tcccagaaac agtcacccca cggacgactg actcgcttct tatggcccga tcgcgcgttg
    10081 aaccgggcat gacgattcct gttgagcggt ttggtaccgg acgagttgtg ggctctgttc
    10141 cggcgggtgg tgccgcccgc cgaggtgaag cgtccacagg gcgggggtcg gcgtcgatcc
    10201 ggtgaccgcg aggcactggc cgcgatcatc ttcgtcgcga cctccggctg tacgtggcgg
    10261 cagctcccgc cggtgttcgg tccggcctgg cagacggtgt acaggaggtt cgcacagtgg
    10321 agtcgggacc cggtctgggc ccggccctac cgcgtggtcc tggatgaact cggtgctcgg
    10381 ggtgagctgg cctggtcgcg gtgcgcgatc gattcggtca gcatccgtgc agtaaaaggg
    10441 ggatgctgac gggaccgaac ccgaccgatc gtggcaagtc aggaacgaac attcacctgc
    10501 tcaccgaccg gaacggactg ccgctgtcgc tgggcatctc cggagccaac atgcacgaca
    10561 gccaggccct ggaaccgctt gtgcgcggca tcccgcccat tcgctcccgc cgcggacctc
    10621 ggcgcagact cccggcgaaa ctgcatgccg acaaggacta tgactacaag cacctgcgac
    10681 gatggctccg cagccgcggc ataccccacc gcatcgcccg caagggcatc gagccctcgc
    10741 agcggctcgg ccgtcatcga tgggtcgttg aacgtacggt ttcctggctt ggcggatgcc
    10801 gtcgcctaca ccgccgctat gaacgcaagc ccgagcactt ccttgccttc gtcggcatag
    10861 ccgccgctct catctgccac cggcgcctca ccacataaac ggtggagagg cgttgtggcg
    10921 tcggcgggat cagtcggtgg tgaagctgga cagtgtcgcg gccgggtatc ggctgcccgc
    10981 cgagccgtgg
//
