LOCUS       NZ_MCOK01000004         3771 bp    DNA     linear   CON 22-OCT-2024
DEFINITION  Nocardiopsis sinuspersici strain UTMC 00102 NOSIN_4, whole genome
            shotgun sequence.
ACCESSION   NZ_MCOK01000004
VERSION     NZ_MCOK01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sinuspersici
  ORGANISM  Nocardiopsis sinuspersici
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Tokovenko,B. and Kalinowski,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2016) Helmholtz Institute for Pharmaceutical
            Research Saarland, Helmholtz Centre for Infection Research, Campus
            C2.3, Saarbrucken 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2016
            Assembly Method                   :: SPAdes v. 3.8.1
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina NextSeq; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001998325.1-RS_2024_10_22
            Annotation Date                   :: 10/22/2024 13:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,351
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 5,224
            CDSs (with protein)               :: 5,224
            Genes (RNA)                       :: 77
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 13 of 50
            Pseudo Genes (incomplete)         :: 44 of 50
            Pseudo Genes (internal stop)      :: 2 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:06:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3771
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCOK01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3771
                     /collection_date="2009-05"
                     /db_xref="taxon:501010"
                     /geo_loc_name="Iran: Sarbandar seaport"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Nocardiopsis sinuspersici"
                     /strain="UTMC 00102"
                     /submitter_seqid="NOSIN_4"
                     /type_material="type strain of Nocardiopsis sinuspersici"
     gene            <1..>3771
                     /locus_tag="NOSIN_RS26555"
                     /old_locus_tag="NOSIN_26205"
     region          1..3771
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3771
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3771
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>3771](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>3771
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>3771
                     /NRPS_PKS="Domain: AMP-binding (223-613). E-value:
                     2.5e-105. Score: 344.3. Matches aSDomain:
                     nrpspksdomains_NOSIN_RS26555_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (718-785). E-value: 1.8e-24. Score:
                     77.7. Matches aSDomain: nrpspksdomains_NOSIN_RS26555_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (798-1089). E-value:
                     9.1e-125. Score: 409.1. Matches aSDomain:
                     nrpspksdomains_NOSIN_RS26555_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1095-1247). E-value: 7.3e-37.
                     Score: 118.5. Matches aSDomain:
                     nrpspksdomains_NOSIN_RS26555_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="NOSIN_RS26555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_26205"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_143833092.1"
                     /sec_met_domain="AMP-binding (E-value: 2.3e-118, bitscore:
                     381.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.8e-14, bitscore:
                     39.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 5.2e-53, bitscore:
                     166.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAREQGLTVNALVQGAWALLLSAHSGDTDVVFGATTSGRPADRPD
                     VENAVGIFINTLPVRVRVTPHTPVNTWLHELQDALTESRQYEHLPLTRLQALSPLPGDT
                     PLFDSLVVFENYPVDEQAARDHGLRVLDVTADEATNYPLTLVAYDGDRIDTQLRYDPDL
                     FDAGTAERLLAHLAFHVESLVTDPASPLRALPPQPGAERDRVTAWGDGGPSRDGLTLVQ
                     LFAEQVRARPDTTALIGDGTEWTYAELDARSDALAVRLHRLGAERGTVVGVSLRRGPDL
                     VAALLAITKAGAAYLPIDPEYPDERQDFIVRDSGIGLLITEESGARRPEPDVAAPPHVT
                     LAEVAAEPVPEDAAAPLPPGPDDLAYVIYTSGSTGTPKGTMVTHRGIGEFAASMAHRFG
                     TEPGWRVLQLASSSFDASVMEVLMAFGAGAALVVPEPGPLVGEELARTLSRHRVNLTII
                     PPSVLASVPPGDFPDLRTLVVGAEACPAELVNRWAPGRRMVNAYGPTEITIAASLSDPL
                     SPGQAPPIGRPVRGARLHVLDHLLRPVPAGVVGELYVGGAGVARGYWGRPGLTVERFVA
                     DPFGSGERLYRTGDLVRWNADGQLGYVGRADAQVKIRGLRIEPGEVESALVALPGVRQA
                     AVTVRQDAPGGPALVGYVSGGADSAEVRDRLIRTLPAHLVPAVVVSVDAVPLTPNGKTD
                     HRALPSPDWAALAGGEYVAPRSEAERVLAGVFGSVLGVDRVGVHDDFFALGGDSILSIQ
                     LVSRARRAGITVTSKQVFARPSVAALAEVAETGRGSAPVPAGPVSGPVTATPIMRWFLD
                     SRAGDPEGFAMSVLLDLAEDFDAGSLPRVAAALLEHHDMLRLRVADDGVPRILPEEDAE
                     TVLETVAGLDETTVEEHTERVRERIDLVSGPVVRMVLFTGEGTPRLLIVAHHLVVDGVS
                     WRILLEDLSTAYRQITGGGAADLGARTTPFPAWAERVAEAVRDGRFDGEAGYWEALAGV
                     PAEVPRDHGHAGNGPVSSQRTVSARLSVETTRALLREVPPVFRTRVNDLLLAALGRVLT
                     GWSGHDRVLIDLEGHGREDLFERVDLSRTVGWFTTVFPVALGSSSDWADQVRTTKEMLR
                     SLPGHGVGFGALRYLGTEQQRTALARVPEPHVGFNYLGRFDTGAESGAPYTAMALGGGD
                     APGADRAHLLDVVGRVAGERLVLDLAYSDLAHTEDTVRGLAEELVAALTDLVEFCLRDG
                     VGGATPSDFPLVGLDQAAVDRLAVPGVQDVLP"
     CDS_motif       16..75
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0001"
                     /evalue="2.90E+00"
                     /label="Cy4"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="25"
                     /protein_start="5"
                     /score="1.4"
                     /tool="antismash"
                     /translation="GLTVNALVQGAWALLLSAHS"
     CDS_motif       85..180
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0002"
                     /evalue="2.90E-17"
                     /label="C5_DCL_263-294"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="60"
                     /protein_start="28"
                     /score="55.0"
                     /tool="antismash"
                     /translation="DVVFGATTSGRPADRPDVENAVGIFINTLPVR"
     CDS_motif       229..351
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0003"
                     /evalue="3.00E-19"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="117"
                     /protein_start="76"
                     /score="61.5"
                     /tool="antismash"
                     /translation="QDALTESRQYEHLPLTRLQALSPLPGDTPLFDSLVVFENYP"
     aSDomain        670..1839
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NOSIN_RS26555_AMP-binding.1"
                     /evalue="2.50E-105"
                     /label="NOSIN_RS26555_AMP-binding.1"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="613"
                     /protein_start="223"
                     /score="344.3"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="FAEQVRARPDTTALIGDGTEWTYAELDARSDALAVRLHRLGAERG
                     TVVGVSLRRGPDLVAALLAITKAGAAYLPIDPEYPDERQDFIVRDSGIGLLITEESGAR
                     RPEPDVAAPPHVTLAEVAAEPVPEDAAAPLPPGPDDLAYVIYTSGSTGTPKGTMVTHRG
                     IGEFAASMAHRFGTEPGWRVLQLASSSFDASVMEVLMAFGAGAALVVPEPGPLVGEELA
                     RTLSRHRVNLTIIPPSVLASVPPGDFPDLRTLVVGAEACPAELVNRWAPGRRMVNAYGP
                     TEITIAASLSDPLSPGQAPPIGRPVRGARLHVLDHLLRPVPAGVVGELYVGGAGVARGY
                     WGRPGLTVERFVADPFGSGERLYRTGDLVRWNADGQLGYVGRADAQVKIR"
     aSModule        670..3741
                     /complete
                     /domains="nrpspksdomains_NOSIN_RS26555_AMP-binding.1"
                     /domains="nrpspksdomains_NOSIN_RS26555_PCP.1"
                     /domains="nrpspksdomains_NOSIN_RS26555_Epimerization.1"
                     /domains="nrpspksdomains_NOSIN_RS26555_TIGR01720.1"
                     /locus_tags="NOSIN_RS26555"
                     /monomer_pairings="Leu -> D-Leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       856..897
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0004"
                     /evalue="8.90E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="299"
                     /protein_start="285"
                     /score="15.8"
                     /tool="antismash"
                     /translation="LAITKAGAAYLPID"
     CDS_motif       1093..1152
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0005"
                     /evalue="1.60E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="384"
                     /protein_start="364"
                     /score="32.8"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGTPKGTMVTH"
     CDS_motif       1633..1722
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0006"
                     /evalue="1.30E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="574"
                     /protein_start="544"
                     /score="52.6"
                     /tool="antismash"
                     /translation="PAGVVGELYVGGAGVARGYWGRPGLTVERF"
     CDS_motif       1804..1869
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0007"
                     /evalue="3.40E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="623"
                     /protein_start="601"
                     /score="35.1"
                     /tool="antismash"
                     /translation="YVGRADAQVKIRGLRIEPGEVE"
     aSDomain        2155..2355
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NOSIN_RS26555_PCP.1"
                     /evalue="1.80E-24"
                     /label="NOSIN_RS26555_PCP.1"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="785"
                     /protein_start="718"
                     /score="77.7"
                     /tool="antismash"
                     /translation="ERVLAGVFGSVLGVDRVGVHDDFFALGGDSILSIQLVSRARRAGI
                     TVTSKQVFARPSVAALAEVAET"
     CDS_motif       2161..2193
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0008"
                     /evalue="5.10E+00"
                     /label="Cy4"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="731"
                     /protein_start="720"
                     /score="0.6"
                     /tool="antismash"
                     /translation="VLAGVFGSVLG"
     aSDomain        2395..3267
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NOSIN_RS26555_Epimerization.1"
                     /evalue="9.10E-125"
                     /label="NOSIN_RS26555_Epimerization.1"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="1089"
                     /protein_start="798"
                     /score="409.1"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="GPVTATPIMRWFLDSRAGDPEGFAMSVLLDLAEDFDAGSLPRVAA
                     ALLEHHDMLRLRVADDGVPRILPEEDAETVLETVAGLDETTVEEHTERVRERIDLVSGP
                     VVRMVLFTGEGTPRLLIVAHHLVVDGVSWRILLEDLSTAYRQITGGGAADLGARTTPFP
                     AWAERVAEAVRDGRFDGEAGYWEALAGVPAEVPRDHGHAGNGPVSSQRTVSARLSVETT
                     RALLREVPPVFRTRVNDLLLAALGRVLTGWSGHDRVLIDLEGHGREDLFERVDLSRTVG
                     WFTTVFPVAL"
     CDS_motif       2533..2580
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0009"
                     /evalue="4.60E-05"
                     /label="Cy2"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="860"
                     /protein_start="844"
                     /score="16.0"
                     /tool="antismash"
                     /translation="LLEHHDMLRLRVADDG"
     CDS_motif       2749..2814
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0010"
                     /evalue="2.00E-12"
                     /label="NRPS-E2"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="938"
                     /protein_start="916"
                     /score="39.2"
                     /tool="antismash"
                     /translation="LLIVAHHLVVDGVSWRILLEDL"
     CDS_motif       3112..3153
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0011"
                     /evalue="4.30E-02"
                     /label="Cy4"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="1051"
                     /protein_start="1037"
                     /score="7.1"
                     /tool="antismash"
                     /translation="LLLAALGRVLTGWS"
     CDS_motif       3217..3267
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0012"
                     /evalue="6.30E-09"
                     /label="NRPS-E5"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="1089"
                     /protein_start="1072"
                     /score="27.8"
                     /tool="antismash"
                     /translation="DLSRTVGWFTTVFPVAL"
     aSDomain        3286..3741
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NOSIN_RS26555_TIGR01720.1"
                     /evalue="7.30E-37"
                     /label="NOSIN_RS26555_TIGR01720.1"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="1247"
                     /protein_start="1095"
                     /score="118.5"
                     /tool="antismash"
                     /translation="ADQVRTTKEMLRSLPGHGVGFGALRYLGTEQQRTALARVPEPHVG
                     FNYLGRFDTGAESGAPYTAMALGGGDAPGADRAHLLDVVGRVAGERLVLDLAYSDLAHT
                     EDTVRGLAEELVAALTDLVEFCLRDGVGGATPSDFPLVGLDQAAVDRL"
     CDS_motif       3304..3366
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS26555_0013"
                     /evalue="1.60E-09"
                     /label="NRPS-E6"
                     /locus_tag="NOSIN_RS26555"
                     /protein_end="1122"
                     /protein_start="1101"
                     /score="29.9"
                     /tool="antismash"
                     /translation="TKEMLRSLPGHGVGFGALRYL"
ORIGIN
        1 ttcgcgcgtg agcagggtct gacggtgaac gcgctggtcc agggcgcgtg ggcgctgctg
       61 ctgtccgccc actccggcga caccgacgtc gtcttcggcg ccaccacctc cggacgcccc
      121 gccgaccgac ccgacgtcga aaacgccgtg ggcatcttca tcaacaccct gcccgtccgc
      181 gtccgcgtca ccccccacac ccccgtcaac acctggctcc acgaactcca ggacgcgctg
      241 accgagtccc gccagtacga gcacctgccc ctcacccggc tccaggcgct ctcccccctg
      301 cccggcgaca cgcccctgtt cgacagcctg gtggtgttcg agaactaccc cgtggacgaa
      361 caggccgccc gggaccacgg actgagggtc ctcgacgtca ccgccgacga ggccaccaac
      421 tacccgctga ccctggtcgc ctacgacggc gaccggatcg acacccagct ccggtacgac
      481 ccggacctgt tcgacgccgg gacggccgag cgcctgctgg cccatctcgc cttccacgtc
      541 gagtcgctgg tcaccgaccc cgcgagcccg ctgcgcgccc tgccgccgca gcccggggcc
      601 gagcgcgacc gcgtcaccgc gtggggcgac ggcggcccct cgcgggacgg gctcacactc
      661 gtccagctct tcgccgagca ggtgcgggcg cgcccggaca cgaccgcgct catcggcgac
      721 gggacggagt ggacctacgc cgaactggac gcgcgcagtg acgccctcgc cgtccgcctg
      781 caccgcctcg gcgccgagcg cggcacggtc gtcggcgtct cgctccggcg cggccccgac
      841 ctcgtcgccg ccctgctcgc gatcaccaag gccggtgccg cctacctgcc catcgacccc
      901 gagtacccgg acgaacgcca ggacttcatc gtgcgtgact ccgggatcgg cctcctcatc
      961 accgaggaga gcggggcccg gcggccggaa ccggacgtcg ccgcgccgcc gcatgtcacc
     1021 ctcgccgaag tggccgccga gccggttccc gaggacgcgg ccgcgccgct cccgcccggg
     1081 ccggacgacc tcgcctacgt catctacacg tccgggtcga ccggaacccc caagggcacc
     1141 atggtgaccc accgggggat cggcgagttc gcggcgtcca tggcccaccg cttcggcacc
     1201 gaacccggat ggcgggtcct gcaactggcc tcgtccagct tcgacgcctc ggtgatggag
     1261 gtcctgatgg ccttcggcgc gggggccgcc ctggtcgtgc ccgaacccgg cccactggtc
     1321 ggtgaggagc tggcgcgaac gctgtcgcgc caccgcgtca acctcaccat catcccgccc
     1381 tccgtcctgg cgtccgtgcc gcccggggac tttccggacc tgcgcacgct ggtggtcggc
     1441 gcggaggcgt gcccggccga gctggtcaac cggtgggctc cgggccgccg catggtgaac
     1501 gcctacgggc cgaccgagat caccatcgcc gcgtcgctca gcgacccgtt gtcccccggg
     1561 caggccccgc cgatcggccg tcccgtccgg ggcgcccgac tgcacgtcct cgaccacctc
     1621 ctgcggccgg tcccggccgg tgtggtcggt gagctgtacg tcggtggcgc gggtgtggcg
     1681 cgcgggtact gggggcggcc ggggctgacc gtggagcggt tcgtcgccga cccgttcggc
     1741 tcgggggagc gcctctaccg cacgggcgac ctggtgcggt ggaacgccga cggccagctg
     1801 gggtacgtgg gccgcgccga cgcgcaggtc aagatccggg gcctgcggat cgagccgggc
     1861 gaggtggagt cggcgctggt cgcgctgccc ggggtccggc aggcggcggt cacggtgcgc
     1921 caggacgccc ccggcggtcc ggcactggtc ggctacgtgt cgggaggggc cgactccgcc
     1981 gaagtgcgtg accggctcat ccggacgctg cccgcgcacc tggtcccggc ggtcgtcgtg
     2041 tccgtggacg ccgttccgct cactcccaac ggcaagaccg accaccgggc gctgccgtcc
     2101 cccgactggg cggcgctggc cgggggcgag tacgtcgctc cgcggtcgga ggcggagcgg
     2161 gtgctggccg gggtgttcgg gtctgtgctg ggtgtggacc gggtcggtgt gcacgacgac
     2221 ttcttcgcgc tgggcggcga ctcgatcctg agtatccagt tggtctcccg ggcccgacgg
     2281 gcggggatca ccgtgacctc caagcaggtc ttcgcccgtc cgtcggtcgc ggccctggcc
     2341 gaggtggccg agaccggtcg gggctcggca ccggtgccgg caggcccggt ctccgggccg
     2401 gtgacggcca ccccgatcat gcggtggttc ctggactccc gtgcgggcga cccggagggg
     2461 ttcgccatgt cggtgctgtt ggacctggcg gaggacttcg acgccggttc tctgcccagg
     2521 gtcgccgccg ccctgctgga gcaccacgac atgctccggc tccgtgtcgc cgacgacggg
     2581 gtcccgagga tcctgccgga ggaggacgcc gaaacggttc tggagaccgt cgcgggtctg
     2641 gacgagacca cggtcgagga gcacacggag cgcgtccgcg agcggatcga cctggtctcg
     2701 ggtccggtgg tgcggatggt gctgttcacc ggtgagggga ctccgcgcct gctgatcgtc
     2761 gcccaccacc tggtcgtgga cggcgtctcc tggcggatcc tgctcgaaga cctctccacc
     2821 gcctaccggc agatcaccgg aggcggtgcc gccgacctgg gggcgcgtac cacgcccttc
     2881 cccgcctggg ccgagcgtgt ggccgaggcc gtgcgggatg ggcgcttcga cggtgaggcc
     2941 gggtactggg aggctctggc cggggtgccc gccgaggtgc cccgtgacca cggccacgcg
     3001 ggcaacgggc cggtctcctc acagcggacc gtgtcggccc ggttgtcggt cgaaacgacc
     3061 cgtgcgctgc tgcgtgaggt cccgccggtc ttccgtaccc gggtcaacga cctgctcctg
     3121 gctgctctgg gccgggtcct caccggttgg tccgggcacg accgcgtcct gatcgacctg
     3181 gaggggcacg gccgcgagga cctgttcgag cgtgtggacc tgtcccgcac cgtcggctgg
     3241 ttcaccaccg tgttccccgt cgccctgggc tcctcctccg actgggccga ccaggtcagg
     3301 accaccaagg agatgctccg ttccctcccc ggccacgggg tcgggttcgg tgccctgcgc
     3361 tatctgggta ccgagcagca gcgcaccgcc ctggcgcggg tccccgagcc ccacgtcggt
     3421 ttcaactacc tcggccgctt cgacaccggc gcggagtccg gggcgcccta caccgccatg
     3481 gccctgggcg gcggcgacgc tcccggagcg gaccgggcgc acctgctcga cgtggtcggc
     3541 cgggtggccg gtgagcggct ggtcctcgac ctggcctact ccgacctggc gcacaccgag
     3601 gacaccgtgc gggggctggc ggaggagctg gtggcggcgc tgaccgacct ggtggagttc
     3661 tgcctccgtg acggtgtcgg cggggccact ccctcggact tcccgctggt cgggctggac
     3721 caggccgcgg tcgaccgtct ggccgtcccc ggtgtccagg acgtcctgcc g
//
