LOCUS       NZ_MCOK01000001        10390 bp    DNA     linear   CON 22-OCT-2024
DEFINITION  Nocardiopsis sinuspersici strain UTMC 00102 NOSIN_1, whole genome
            shotgun sequence.
ACCESSION   NZ_MCOK01000001
VERSION     NZ_MCOK01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sinuspersici
  ORGANISM  Nocardiopsis sinuspersici
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 6071583)
  AUTHORS   Tokovenko,B. and Kalinowski,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2016) Helmholtz Institute for Pharmaceutical
            Research Saarland, Helmholtz Centre for Infection Research, Campus
            C2.3, Saarbrucken 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2016
            Assembly Method                   :: SPAdes v. 3.8.1
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina NextSeq; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001998325.1-RS_2024_10_22
            Annotation Date                   :: 10/22/2024 13:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,351
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 5,224
            CDSs (with protein)               :: 5,224
            Genes (RNA)                       :: 77
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 13 of 50
            Pseudo Genes (incomplete)         :: 44 of 50
            Pseudo Genes (internal stop)      :: 2 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:06:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5971971
            Orig. end                         :: 5982361
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCOK01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="14"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5390
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(31..45)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.0"
                     /tool="antismash"
     gene            complement(456..1139)
                     /locus_tag="NOSIN_RS27405"
                     /old_locus_tag="NOSIN_25645"
     CDS             complement(456..1139)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017542313.1"
                     /locus_tag="NOSIN_RS27405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25645"
                     /product="copper resistance CopC family protein"
                     /protein_id="WP_170293739.1"
                     /transl_table=11
                     /translation="MTTDHSIPTRGGTRPSRLLPRAAAALLTPLAAAALALAPSPALAH
                     DALTGSDPEDGQTLDSVPEEVVLTFSNAPMEGGSGSAVVVTGPDEETTYEEGEPTFDGT
                     DVSVGLRTLDQAGEYTIGFRVVSSDGHPIQDTLTFSVTEEAVAAAAPESAPEETAPEEE
                     AATGAAEEETAGEPAGEAPADEAAAAEEVSPVALAVLAVVAVGGVAAVVLVAVRMRKRS
                     GAGRE"
     gene            complement(1169..1747)
                     /locus_tag="NOSIN_RS27410"
                     /old_locus_tag="NOSIN_25650"
     CDS             complement(1169..1747)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590333.1"
                     /locus_tag="NOSIN_RS27410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25650"
                     /product="copper resistance protein"
                     /protein_id="WP_170293740.1"
                     /transl_table=11
                     /translation="MRPAHGTLRLLRTALLAAACTGLAWSGHNVWAASPASAGGFAAAA
                     AVLFPLLWYFTRTMRGFGDIFAVMASVQVLLHLILLGSAQPLPGIPGAGADHAGHGLSA
                     HALGLAPGMLFAHLWAALLASALLAHGEAALWFLAALLCRALPSLRVPDLAFTTPVRIP
                     CAAVPTAPSPVVLSANGPRGPPRPRGAFA"
     gene            complement(1936..2205)
                     /locus_tag="NOSIN_RS25975"
                     /old_locus_tag="NOSIN_25655"
     CDS             complement(1936..2205)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573107.1"
                     /locus_tag="NOSIN_RS25975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25655"
                     /product="WhiB family transcriptional regulator"
                     /protein_id="WP_077693241.1"
                     /transl_table=11
                     /translation="MNRLYHESEDWVEQGNCRSYDPEIFFPVSTTGIGRADTEQAVAVC
                     RSCTVRQECLRWALRAGEAHGVWGGTTPEQRRYMRRELVPAGRS"
     misc_feature    complement(2616..2633)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.81"
                     /tool="antismash"
     gene            3018..3518
                     /gene="ectA"
                     /locus_tag="NOSIN_RS25980"
                     /old_locus_tag="NOSIN_25660"
     CDS             3018..3518
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610152.1"
                     /locus_tag="NOSIN_RS25980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25660"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_077693242.1"
                     /transl_table=11
                     /translation="MRLSHPSLEDGPHMWRLAGETGMDVNSPYAYALWCRDFAETSVVA
                     RDDEGRVAAYVTGFVRPDSPDTYFLWQVAVDSAFRGQRLARRMLDFIGDRITERGLRYL
                     EATVTADNTASRALFASFARDRGAEAVWSPLFGGEHFPADDDTPHDPEDLVRIGPLNPE
                     PEV"
     gene            3606..4871
                     /gene="ectB"
                     /locus_tag="NOSIN_RS25985"
                     /old_locus_tag="NOSIN_25665"
     CDS             3606..4871
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-357). E-value: 4.3e-72.
                     Score: 234.9. Matches aSDomain:
                     nrpspksdomains_NOSIN_RS25985_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610153.1"
                     /locus_tag="NOSIN_RS25985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25665"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_077693243.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.4e-73, bitscore:
                     245.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTFQRLESEVRGYCRNWPVVFDRAVGSHVYDETGKPFLDFFAGA
                     GSLNYGHNNPELKTSLIEYLTDDKIVHSLDAYSVAKREFLKTFEEIILKPRNLDYKIQF
                     PGPAGNNAVEAALKLARKYTGRETIVNFTNGFHGMTLGALAVTGNSMKRGGAGVPLGHV
                     ATMPFDNYMDGKTPDFLWLRSLLDDSGSGLDKPAAVIVETVQGEGGINAASAQWLRDLS
                     ELCREYEILLIVDDIQMGCGRTGDFFSFEEAGIVPDIVTLSKSISGYGLPMALTLFKRE
                     LDVWEPGEHNGTFRGFNPAMVTGSEALRRYWSDSSFADSVKAKGDMVAARLAEMAAEHA
                     EFGAHVRGRGLARGLAFEQDGIAKKVAAESFDRGLLLETSGPEDEVAKLLPPLTASEEE
                     LTAGLDIMADAVRAAVKVAQPA"
     aSDomain        3672..4676
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NOSIN_RS25985_Aminotran_3.1"
                     /evalue="4.30E-72"
                     /label="NOSIN_RS25985_Aminotran_3.1"
                     /locus_tag="NOSIN_RS25985"
                     /protein_end="357"
                     /protein_start="22"
                     /score="234.9"
                     /tool="antismash"
                     /translation="DRAVGSHVYDETGKPFLDFFAGAGSLNYGHNNPELKTSLIEYLTD
                     DKIVHSLDAYSVAKREFLKTFEEIILKPRNLDYKIQFPGPAGNNAVEAALKLARKYTGR
                     ETIVNFTNGFHGMTLGALAVTGNSMKRGGAGVPLGHVATMPFDNYMDGKTPDFLWLRSL
                     LDDSGSGLDKPAAVIVETVQGEGGINAASAQWLRDLSELCREYEILLIVDDIQMGCGRT
                     GDFFSFEEAGIVPDIVTLSKSISGYGLPMALTLFKRELDVWEPGEHNGTFRGFNPAMVT
                     GSEALRRYWSDSSFADSVKAKGDMVAARLAEMAAEHAEFGAHVRGRGLARGLAF"
     gene            5001..5390
                     /locus_tag="NOSIN_RS25990"
                     /old_locus_tag="NOSIN_25670"
     CDS             5001..5390
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567884.1"
                     /locus_tag="NOSIN_RS25990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25670"
                     /product="ectoine synthase"
                     /protein_id="WP_077693244.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.3e-55, bitscore:
                     182.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLDEVNDTDADIKTETWRSRRIVLAKDKVGFSFHETVLYAG
                     TETSMWYANHIELVHCIEGEAEVTNDETGEKHIITPGTLYLLNGHEKHTVRPKTDFRVL
                     CVFNPPVTGREVHDENGVYPLIVED"
     misc_feature    5614..5629
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.35"
                     /tool="antismash"
     gene            6045..6896
                     /gene="ehuB"
                     /locus_tag="NOSIN_RS25995"
                     /old_locus_tag="NOSIN_25675"
     CDS             6045..6896
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573111.1"
                     /locus_tag="NOSIN_RS25995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25675"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_077693245.1"
                     /transl_table=11
                     /translation="MGLATLGLAACSRVDGGGGEGEEGTSTLDRLREQGFVAVGLANEI
                     PFGFVDDSGNPAGQSPAVAKAVFEELGVPEIQASSVNWDGLIPGLKSNRFDVIAAGMFI
                     TPERCGQVAFTEPTATSPEAFMVKEGNPEGIKHFKDFAENPDLKLAVLNGSVEQTYANY
                     FEVPEDQQELIPNQTAGYELLEAGRVDAMSMLSVSHEYLLQDRGGPFEVTEPFFPVIDG
                     QEEKGWGGLCVRQEDTTLLEEMNRVIAEFKESGRLLELGQDWGFTEADLPDDMTTAELC
                     KE"
     gene            6904..7629
                     /gene="ehuC"
                     /locus_tag="NOSIN_RS26000"
                     /old_locus_tag="NOSIN_25680"
     CDS             6904..7629
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156203.1"
                     /locus_tag="NOSIN_RS26000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25680"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_077693246.1"
                     /transl_table=11
                     /translation="MDFLIQRLPLYIDGAWITIQLTFGGCALAFVLAMVFGIMGTIRHW
                     LPRAISTVYVEVFRGIAALVLMFWMAFALPQLSGFRLDDMFAAILALGLNVGAYGAEVV
                     RSSINAVPKAQVEATVALNLSWFQRTRLVVLPQAWAQMLPTFGNLVIELMKATAVVYLI
                     GVADLTAVAQNMRESTGETVVAFIGAFILYYLIAQVLIFGMRMLERRANRKLGRTPPRG
                     KSLKSLAQSTPVGSAGGTF"
     gene            7626..8264
                     /gene="ehuD"
                     /locus_tag="NOSIN_RS26005"
                     /old_locus_tag="NOSIN_25685"
     CDS             7626..8264
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156204.1"
                     /locus_tag="NOSIN_RS26005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25685"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_077693247.1"
                     /transl_table=11
                     /translation="MMWDFQWTLQILPDLLGALGTTVQITFLSYLIALVLGLLIAIARR
                     TPFFGAVLHAATEFIRTTPLVIQLVFVFLLTPAHITPMTVGVVVLGVHYAAYTAEVYRS
                     GIEGVAKGQWEAARALSLPARRTWGAIVLPQAIRKVIPALGNYLIATFKDTPLLIAIGV
                     AELLTEAQRIGSASFRVVEVYTVVGFLFLLVSIPSAILLRRLERRYAAV"
     gene            8251..9075
                     /gene="ehuA"
                     /locus_tag="NOSIN_RS26010"
                     /old_locus_tag="NOSIN_25690"
     CDS             8251..9075
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610157.1"
                     /locus_tag="NOSIN_RS26010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25690"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_077693248.1"
                     /transl_table=11
                     /translation="MPPSDTPVNAESPGVEGQSLIVFDDVVKRFGDLTVLDDLNFSVAP
                     GERVTLIGPSGSGKTTILRLLMTLEKITEGVIWVGGEPFSHMRRGQGLVRASEGYMRER
                     RKRIGMVFQQFNLFPNMSVRQNITEAPVHVLGTPKDEANARAKELLDLVGLGEKIDAHP
                     TQLSGGQQQRVAIARALAMQPEILLLDEVTSALDPELVAGVLDVLREVAETTDITMLCV
                     THEMGFARDVSHRVLMFDQGQIAEEGPPEQIFGDPKEDRTKSFLRSVLDNGF"
     gene            9250..10182
                     /locus_tag="NOSIN_RS26015"
                     /old_locus_tag="NOSIN_25695"
     CDS             9250..10182
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546875.1"
                     /locus_tag="NOSIN_RS26015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_25695"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_171986641.1"
                     /sec_met_domain="Abi (E-value: 1.4e-11, bitscore: 43.0,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRRWGLAWGALLAVLLIAVPMLSHPALRSDVLGDSMVLLMVSWS
                     PMLIGGILAWALVRGFGARDRLDLRIVRAMEGHPVGREVAWLGLLLVCFVLSMVALSLL
                     FRAYGAQIGADLAMSASLVSRLLFLFTLPLLLMDRSGVTVDGKGTDMPAIALRVSEPWR
                     WLGLIPVGVALTLLGYLLIPYIGLPGPSLPLLGFLLAFTVIAVCEEIFFRGMMQSRLEL
                     QMGRWGGIVATSVVFAVTYAVIQPYDAVSQLPGTDLVYNTGLALLTYATASLLYGYLWT
                     CFRNTWLQVIMRVGMFLMIMPPDLQIGLN"
ORIGIN
        1 ccagcgcctg gctcatgtgc agcgacggcc tgtccgacct ggtcctgacc gaggagatgg
       61 agcggatgat cgccgaggcg ggcagcgacg aggccgccgt gcacgccctg tggcaggccg
      121 ccatggaggc gggcggcaag gacaacatca gcatcctgct ggcccggcgc gtctgatccc
      181 gcacccgacc ccgaccccga cgaacacgcc ccccgtccat ccccgtccgc gcttcccgcc
      241 gtttccacgg cttccgccac ctccggagct cccacacccc cgccccccac acggcttcca
      301 ccgttcccgc gcttctccac cggccaccgc ggcctgtccg tgactcctgc cctccgccgc
      361 cggggcgccg gacacggaag gggcccgtcc accgtctccg agtccgggaa cggtgtacgg
      421 gcccctcgtc cgtcgccggc cgcaggcggc ttcgcctact cccggcccgc gccggagcgc
      481 ttgcgcatgc gcacggccac gagtacgacc gcggcgacac cgccgacggc gacgacggcc
      541 agtaccgcca gggcgacggg cgagacctcc tccgcggccg cggcctcgtc ggcgggcgcc
      601 tccccggccg gttccccggc ggtctcctcc tccgcggccc cggtcgccgc ctcctcctcg
      661 ggagcggtct cctcgggcgc ggactccgga gccgccgcgg cgacggcctc ctcggtcacc
      721 gagaacgtca gggtgtcctg gatcgggtgt ccgtcggagg agaccacgcg gaagccgatc
      781 gtgtactcgc cggcctggtc cagcgtgcgc agccccacgg acacgtccgt gccgtcgaag
      841 gtgggctcgc cctcctcgta ggtggtctcc tcgtccgggc ccgtgacgac gacggcactg
      901 ccgctgccgc cctccatcgg ggcgttgctg aaggtcagca cgacctcctc ggggacgctg
      961 tccagggtct ggccgtcctc ggggtcggaa ccggtcaggg cgtcgtgggc cagggcggga
     1021 gagggggcca gcgccagggc ggcggccgcg agcggggtca gcagggcggc ggccgcacgc
     1081 ggcagcaggc gggagggtct tgtgccgccg cgggtgggga tgctgtggtc agtggtcatg
     1141 tcttctctca gcgttcggtt cggtcggtct acgcgaaggc gccccggggc cggggaggtc
     1201 cgcggggacc gttcgccgag agcacgacgg gggagggcgc cgtcgggacg gcggcacacg
     1261 ggatccgtac gggggtggtg aacgcgaggt cgggcacgcg cagcgacggc agcgcgcggc
     1321 agagcagggc ggccaggaac cacagggccg cctcgccgtg ggcgagcagc gcggaggcca
     1381 gcagggcggc ccacaggtgt gcgaacagca tgcccggcgc taggccgaga gcgtgggcgg
     1441 acaggccgtg tccggcgtgg tccgcgccgg cgccgggtat ccccgggagc ggctgggccg
     1501 atcccaggag gatcaggtgc aggaggacct ggacggaggc catgacggcg aagatgtcgc
     1561 cgaagccgcg catcgtgcgg gtgaagtacc acagcagggg gaacaggacc gcggccgcgg
     1621 ccgcgaaccc gccggcggag gccggggacg cggcccacac gttgtggccg ctccaggcaa
     1681 gccccgtgca cgcagcggcg aggagtgccg tgcgcaggag gcggagtgtg ccgtgggcgg
     1741 gtcgcacgag aaagaaccta ggccgtgccc cacggggccg caaggtgccg cggggtcacc
     1801 ggtcgcgcac cgcgtggacc ggcccggcgg gccggggcgg cgctcgtccg cgtcatggtg
     1861 tctccctcgg aacggggtcg ggcggggagc gggaaggcgg ggggccaccg ggcgcggccc
     1921 cccgggcgct cccgatcagc tcctgccggc cggaaccagc tcgcgccgca tgtagcggcg
     1981 ctgctcgggg gtggtgccac cccagacccc gtgggcctct ccggcccgca gggcccaccg
     2041 caggcactcc tggcgaacag tacaactgcg gcagacggcg acggcctgct cggtgtcggc
     2101 gcgcccgatc ccggtggtgc tcaccgggaa gaagatctcc gggtcgtacg agcggcagtt
     2161 gccctgctcg acccagtcct cgctctcgtg gtacaggcgg ttcacgggcg cctccgatcc
     2221 gtggctgtga tccgtggtca gtggcccgca ccgtgctctc acggcggggt caggaatcct
     2281 ccgccccgcc gttcacgggc ggtcggcccg gagaatcgcg gcgcagcgat gcgaccaagt
     2341 gtagtacgac cttgtgtagt actacgcgaa gtctgctaga aaatccgtga tttctcaagg
     2401 acgttccggg tagcgggcca ctgtgtcccg cagtggacgc gggcgaggag gtcggcggtg
     2461 ccccgtcgcg gcggctcccg ggccgcgccg gaccgccgga tcctcgcgcc caccggcgct
     2521 tcggcgatcc ttccggtgcc ccggaacgta ccgtgtggcg tgaaacgcgg cccgggcccc
     2581 gttatgtcgc gaggtttcca ccgaatcaat gtgacttaac gcacgcttaa tattgcctgt
     2641 tgagggccta tgtttggccc tgagggttgt tgacctcatc aggtggccgc cgcgcggtgc
     2701 gcgcccgcgc cccgggtgat ccgcccgtgg ccgtgacgcg aaatcccctg atgggcaagg
     2761 cgtgagcgcg tcggcggacc gcctcggagc ttcggacgaa ggccctgttc agggcgtcga
     2821 agcactgtta aatgaacaag tcctacccga tgagagcgtg tcgaaacatc tacacggagc
     2881 ctccctgaaa tctcgttggt attcgttcgc taagttcgat cgacgtgcca catcaacgaa
     2941 cgaacctcca agcacgcgac cgcactgacg ccggggcccc gagcaccgag acaccaggag
     3001 ccgcgggccg tggaggcgtg cgcctgtcgc acccgtccct ggaggacgga ccgcacatgt
     3061 ggcggctggc gggggagacg ggcatggacg tcaactcccc gtacgcctac gcgctctggt
     3121 gccgggactt cgccgagacc agcgtcgtcg cccgcgatga cgagggccgt gtcgcggcct
     3181 acgtcaccgg cttcgtgcgg cccgacagcc ccgacacgta cttcctctgg caggtggccg
     3241 tggactccgc gttccgcgga cagcggctgg cccgccggat gctcgacttc atcggtgacc
     3301 ggatcaccga gcgtggcctg cgctacctcg aagcgaccgt caccgccgac aacaccgcat
     3361 cccgagccct gttcgcctcg tttgcccggg acaggggggc cgaagccgtg tggagtccgc
     3421 tcttcggcgg ggaacacttc cctgcggacg acgacacacc gcacgatccc gaagacctcg
     3481 tacgcatcgg tcccctcaat cccgagccgg aagtgtgacc gaccatccgc accggatcca
     3541 ccaacccacc ccatcgtcca agccatgagc ccacgaacct ggggaaagga accaaggaac
     3601 acaccatgga taccttccag cgcctggagt ccgaagtccg cggatactgc cggaactggc
     3661 cggtcgtgtt cgacagggcc gtcggaagcc atgtctacga cgagaccggc aagcccttcc
     3721 tggacttctt cgcaggagcg ggttcgctca actacgggca caacaacccg gagctgaaga
     3781 cctcgctcat cgagtacctg accgacgaca agatcgtcca cagcctcgac gcctacagcg
     3841 tggccaaacg cgagttcctg aaaaccttcg aggagatcat cctcaaaccg cggaacctgg
     3901 actacaagat ccagttcccg ggccccgcgg gcaacaacgc ggtcgaggcc gcgctgaagc
     3961 tggcccgcaa gtacaccggt cgcgagacca tcgtcaactt caccaacggc ttccacggca
     4021 tgaccctggg cgccctggcc gtcaccggca actcgatgaa gcgcggtggc gcgggcgtgc
     4081 ccctgggcca cgtcgccacg atgccgttcg acaactacat ggacggcaag acgccggact
     4141 tcctgtggct gcgcagcctg ctggacgaca gcggcagtgg cctggacaag cccgccgccg
     4201 tcatcgtcga gacggtccag ggcgagggcg gcatcaacgc cgccagcgcc cagtggctgc
     4261 gcgacctctc ggagctgtgc cgcgagtacg agatcctgct gatcgtcgac gacatccaga
     4321 tgggctgcgg ccgtaccggg gacttcttca gcttcgagga agccgggatc gtcccggaca
     4381 tcgtcaccct gtccaagtcc atcagcggct acggcctgcc catggccctc accctgttca
     4441 agcgcgagct ggacgtgtgg gagccgggtg agcacaacgg caccttccgc gggttcaacc
     4501 cggccatggt caccggctcg gaggcgctgc gccgctactg gagcgactcc tcgttcgccg
     4561 actcggtcaa ggccaagggc gacatggtcg ccgcgcgcct ggccgagatg gccgccgagc
     4621 acgccgagtt cggcgcgcac gtgcgcggcc gcggcctggc ccgcggcctg gccttcgagc
     4681 aggacggcat cgccaagaag gtcgccgccg agtccttcga ccgcggcctg ctcctggaga
     4741 cctccggtcc cgaggacgag gtcgccaaac tcctgccgcc gctgacggcg agcgaggagg
     4801 agctcacggc gggacttgac atcatggctg acgcggtccg tgccgcggtc aaggtggcac
     4861 agcccgccta ggaacttcct ctgcggagtt ccacaccacg cggtgcgggg ccggcggcga
     4921 ccttcgggac gccgtccggc cccgcccccg tgtcgcacca ccatcgaaag gcgccgcaga
     4981 gcgcgtaaag gagacaacca gtgatcgttc gcagcctgga cgaggtcaac gacaccgacg
     5041 ccgacatcaa gacggagacc tggcgcagcc gccggatcgt cctcgcgaag gacaaggtcg
     5101 gcttctcctt ccacgagacc gtcctgtacg cgggtacgga gaccagcatg tggtacgcca
     5161 accacatcga actcgtgcac tgcatcgagg gcgaggccga ggtgaccaac gacgagacgg
     5221 gggagaagca catcatcacc ccgggcaccc tctacctgct caacggtcac gagaagcaca
     5281 ccgtccgccc caagacggac ttccgcgtgc tgtgcgtgtt caacccgccc gtcaccgggc
     5341 gcgaggtcca cgacgagaac ggcgtctacc cgctcatcgt cgaggactga cgacagcacc
     5401 ggagagcccg gtccgggccc gggacccgag gtcggggtcc tggggcacga ggcccgggcc
     5461 cggtcccgca ccgggaaccg ccggggggtg ggttcccgtc ctggaggcgt ccggacggca
     5521 ggggcacgcc acgggcgtct ccttccggga ccctccgagg agcgggcgag acgagcagga
     5581 cgaacacact gtgggcgggg tcgcgagacc ccgcccacag tgctttcacg ggggaggcgc
     5641 cccagggtga cggagcctcg gactcagcgt cacaaagcac aggtgacccg ccctgagcag
     5701 ggcgaagatg cgatcacccg ggggccgcgc acgcataacc agtgcataac ggactaatcg
     5761 ggtctcgtta ctgtcgagca tgcttggggg aggctgagca tgctcgggac agaaccccgt
     5821 accaggccgt cgtgccctgc accggacggc ggccgcagcc gcggtacagc gggccgcgac
     5881 ccgcccacac cgggcgtccc accgatccgc atgtgcggcg tagccgtccc cccggacgag
     5941 tgcgccgctc cccccaagtc tttcgaggag cagtcgtgaa gaagcaggta ccagctgtca
     6001 gccgaggagc ctggggccgc cgagacgcac ttcgcctggg tggagtgggg ctggccaccc
     6061 tcggtctcgc cgcctgcagc cgggtcgacg gcggaggggg agagggggag gagggcacat
     6121 cgaccctgga ccgcctccgc gagcagggct tcgtggcggt cggcctcgcc aacgagatcc
     6181 ccttcggctt cgtcgacgac tccggcaacc ccgccgggca gtcccccgcc gtggccaagg
     6241 ccgtcttcga ggagctgggc gtccccgaga tccaggcctc ctccgtgaac tgggacggac
     6301 tgatcccggg cctgaagtca aaccgcttcg acgtcatcgc cgccggcatg ttcatcaccc
     6361 ccgagcgctg cgggcaggtc gccttcaccg agcccaccgc cacctccccc gaggccttca
     6421 tggtcaagga gggcaacccg gagggcatca agcacttcaa ggacttcgcc gagaaccccg
     6481 acctcaagct cgcggtcctc aacggctcgg tcgagcagac ctacgccaac tacttcgagg
     6541 tgcccgagga ccagcaggag ctcatcccca accagaccgc cggctacgag ctgctggagg
     6601 cggggcgcgt cgacgcgatg tccatgctga gcgtgtccca cgagtacctc ctccaggacc
     6661 ggggcggccc cttcgaggtc accgagccgt tcttccccgt gatcgacggc caggaggaga
     6721 agggctgggg cggcctgtgc gtccggcagg aggacaccac gctgctggag gagatgaacc
     6781 gggtgatcgc cgagttcaag gagagcggac gcctcctgga gctgggccag gactggggct
     6841 tcaccgaggc cgacctgccc gacgacatga ccaccgccga actctgcaag gaatgaaccc
     6901 gcagtggact tcctgatcca gcgactaccg ctctatatcg acggcgcgtg gatcaccatc
     6961 cagctcacct tcggcggctg tgccctggcc ttcgtcctgg ccatggtctt cgggatcatg
     7021 gggacgatca ggcactggct gccccgggcc atctccacgg tctacgtgga ggtgttccgg
     7081 ggcatcgccg ccctggtgct gatgttctgg atggcgttcg ccctgccgca gctgagcggg
     7141 ttccgactgg acgacatgtt cgccgcgatc ctcgccctgg gcctcaacgt gggggcctac
     7201 ggagccgagg tcgtgcggtc gtccatcaac gccgtgccca aggcgcaggt cgaggccacg
     7261 gtcgcgctga acctctcctg gttccagcgg acgcgcctcg tcgtgctgcc ccaggcgtgg
     7321 gcccagatgc tgcccacgtt cggcaacctc gtcatcgagc tgatgaaggc caccgcggtg
     7381 gtctacctca tcggtgtggc cgacctgacg gccgtcgcgc agaacatgcg cgagtccacg
     7441 ggtgagacgg tggtcgcctt catcggcgcc ttcatcctct actacctgat cgcgcaggtc
     7501 ctcatcttcg gcatgcgaat gctcgaacgc cgcgccaacc ggaagctggg ccgtaccccg
     7561 ccgcgcggca agagcctgaa gtccctcgcc cagtccacac cggtcggtag cgcgggaggg
     7621 accttctgat gtgggacttc caatggaccc tccagatcct gcccgacctg ttgggcgccc
     7681 tggggacgac cgtccagatc accttcctca gctacctgat cgctctggtc ctggggctgc
     7741 tgatcgccat cgcccgccgg acgccgttct tcggcgccgt cctgcacgcg gccacggagt
     7801 tcatccgtac gacaccgctg gtcatccaac tggtgttcgt cttcctcctg accccggccc
     7861 acatcacccc gatgaccgtg ggcgtcgtcg tcctcggcgt gcactacgcg gcctacacgg
     7921 ccgaggtcta ccgctcgggc atcgagggcg tggccaaggg gcagtgggag gcggcccggg
     7981 cactgagcct gcccgcccgc cgcacctggg gtgccatcgt gctgccgcag gccatccgca
     8041 aggtcatccc cgcgctgggc aactacctca tcgcgacgtt caaggacacg ccgctgctca
     8101 tcgcgatcgg tgtggccgag ctgctcaccg aggcccagcg gatcggcagc gcgagcttcc
     8161 gcgtcgtcga ggtctacacc gtggtcggat tcctgttcct cctcgtgagc atcccgtccg
     8221 ccatcctcct ccgacgattg gagcgacgct atgccgccgt ctgatacccc cgtgaacgca
     8281 gagtccccgg gtgtcgaggg ccagtccctg atcgtcttcg acgacgtggt caagcgcttc
     8341 ggcgacctca ccgtgctcga cgacctgaac ttctccgtcg ctcccggcga gcgggtcacc
     8401 ctcatcggcc cgagcggttc gggcaagacc accatcctgc gcctgctgat gaccctggag
     8461 aagatcaccg agggcgtcat ctgggtgggc ggcgagccgt tcagccacat gcgccggggc
     8521 caggggctgg tccgtgcgag cgagggctac atgcgcgagc gccgcaagcg catcggcatg
     8581 gtcttccagc agttcaacct gtttccgaac atgagcgtgc gccagaacat caccgaggcg
     8641 ccagtacacg tgctgggcac cccgaaggac gaggccaacg cccgggcgaa ggagctgctg
     8701 gacctggtcg ggctcggcga gaagatcgac gcccacccca cccagctctc cggcgggcag
     8761 cagcagcgcg tggcgatcgc ccgcgcgctg gccatgcagc cggagatcct gctgctggac
     8821 gaggtcacct cggccctgga cccggagctg gtcgccggtg tcctggacgt gctgcgcgag
     8881 gtcgccgaga ccacggacat cacgatgctg tgcgtgaccc acgagatggg cttcgcgcgg
     8941 gacgtgtcgc accgggtgct catgttcgac cagggccaga tcgccgagga gggtccgccg
     9001 gagcagatct tcggcgatcc caaggaggac cgcaccaagt ccttcctccg ctcggtcctg
     9061 gacaacggct tctgagggcc ggcgcgtacg ccgaccgacg cggggccgct tccggagccg
     9121 ggagcggccc cgcgccacgt cctgacctgg tatgccgaga cgagtgacga cataccgtag
     9181 tcattaagta gcttttagtt tccccttggg cagaatgaga gttctgcgac aggagggact
     9241 cttgtgacca tggtgcgaag atggggactc gcctgggggg cgttgctcgc cgtcctgctc
     9301 atcgcggtac cgatgctctc gcaccccgcg ttgcgctccg acgtcctggg cgactccatg
     9361 gtcctcctca tggtctcctg gtccccgatg ctcatcggcg ggatcctggc ctgggccctg
     9421 gtcaggggtt tcggcgcacg cgaccggctg gacctcagga tcgtccgggc gatggagggc
     9481 caccccgtcg ggcgcgaggt ggcctggctg gggctgctcc tcgtctgctt cgtgctgtcc
     9541 atggtggcgc tgtccctgct ctttcgcgcc tacggggcgc agatcggcgc ggacctggcc
     9601 atgtccgcct cgctggtgtc caggcttctg ttcctgttca ccctgccgct gctgctcatg
     9661 gaccgctcag gggtcaccgt ggacggcaag ggcaccgaca tgcccgcgat cgcgctgagg
     9721 gtgtccgagc cctggcgctg gctcgggctg atcccggtcg gggtcgcgct gaccctcctc
     9781 ggctacctgc tcatccccta catcgggctg ccggggccgt ccctccccct gctgggtttc
     9841 ctgctcgcct tcaccgtgat cgccgtgtgc gaggagatct tcttccgcgg gatgatgcag
     9901 agccgactgg agctccagat gggccgctgg ggcgggatcg tggcgacctc ggtggtcttc
     9961 gcggtcacct acgcggtcat ccagccctac gacgcggtct cccagcttcc ggggaccgac
    10021 ctggtgtaca acaccggcct cgccctgctg acctacgcca cggccagcct cctctacggc
    10081 tacctgtgga cgtgctttcg caacacctgg ctccaggtga tcatgcgcgt cggcatgttc
    10141 ctgatgatca tgccgcccga cctgcagatc ggtctcaact gagggccgcg ccgacaccgg
    10201 ccacggcccc accgttaccg gccgacgact ccgccggcgc cggtcctccc cggaaccggc
    10261 ccgccccgca cgccgggtac acgcggcgaa gaccccggcg tccccggcgc gtcggcggaa
    10321 gaggtccaca gcggtcgcgt ggcggtcacc tcgccctcac ggaggtgtca ccgggaggtc
    10381 atggaaaaga
//
