LOCUS       NZ_MCOK01000001        11395 bp    DNA     linear   CON 22-OCT-2024
DEFINITION  Nocardiopsis sinuspersici strain UTMC 00102 NOSIN_1, whole genome
            shotgun sequence.
ACCESSION   NZ_MCOK01000001
VERSION     NZ_MCOK01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sinuspersici
  ORGANISM  Nocardiopsis sinuspersici
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 6071583)
  AUTHORS   Tokovenko,B. and Kalinowski,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2016) Helmholtz Institute for Pharmaceutical
            Research Saarland, Helmholtz Centre for Infection Research, Campus
            C2.3, Saarbrucken 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2016
            Assembly Method                   :: SPAdes v. 3.8.1
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina NextSeq; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001998325.1-RS_2024_10_22
            Annotation Date                   :: 10/22/2024 13:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,351
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 5,224
            CDSs (with protein)               :: 5,224
            Genes (RNA)                       :: 77
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 13 of 50
            Pseudo Genes (incomplete)         :: 44 of 50
            Pseudo Genes (internal stop)      :: 2 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:06:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4588623
            Orig. end                         :: 4600018
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCOK01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11395
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11395
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11395
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6395](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6395
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    193..209
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     misc_feature    389..409
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.68"
                     /tool="antismash"
     gene            635..1594
                     /locus_tag="NOSIN_RS19795"
                     /old_locus_tag="NOSIN_19565"
     CDS             635..1594
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152848.1"
                     /locus_tag="NOSIN_RS19795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_19565"
                     /product="IS481 family transposase"
                     /protein_id="WP_077692188.1"
                     /transl_table=11
                     /translation="MPHTTHANARLTPAGRLALARCVIDQGWPLRRAAERFQTSTTTAK
                     RWADRYRATGEAGMVDASSRPARSPRRTRPRRERRVIKLRHLRRWGPARIGGHLGMHAS
                     TVHRILTRYGCARLAHTDRATGRAVRRYERERPGELVHVDIKKLGNIPHGGGHKALGRP
                     RGRRHRSNAGYAYLHNAVDDHSRLAYSEILTDEKKETAVAFWERAHAYFASVGITVERV
                     LTDNGSCYRSRLWRDLVRDQGVKHKRTRPYRPQTNGKVERFNRILVQEWAYARPYASES
                     QRREAFPGWLHHYNHHRFHTALGGPPASRVTNLSGQYS"
     misc_feature    654..667
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.03"
                     /tool="antismash"
     gene            complement(1577..1885)
                     /locus_tag="NOSIN_RS19800"
                     /old_locus_tag="NOSIN_19570"
     CDS             complement(1577..1885)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NOSIN_RS19800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NOSIN_19570"
                     /product="hypothetical protein"
                     /protein_id="WP_227015285.1"
                     /transl_table=11
                     /translation="MLRFEPVRDWELVSRVHLRSWLVRLRHRQHKDAYVNNQYRCLQQF
                     FKWLTDEEEIANPMRKMKPPKVTPEPVPVFEQDEIIRILSTAEGRDFLARRDLAVLP"
     gene            complement(1899..2111)
                     /locus_tag="NOSIN_RS27850"
     CDS             complement(1899..2111)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NOSIN_RS27850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_227015286.1"
                     /transl_table=11
                     /translation="MATGRIRRGPRNLAAGPLRTHVDSFVLHLRSEGKRDNTVTMYADA
                     VRWFAAEHLLTGGQPTDRHPLRHRR"
     tRNA            complement(2189..2261)
                     /anticodon=(pos:complement(4590849..4590851),aa:Thr,
                     seq:tgt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="NOSIN_RS19805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="NOSIN_19575"
                     /product="tRNA-Thr"
     gene            complement(2189..2261)
                     /locus_tag="NOSIN_RS19805"
                     /old_locus_tag="NOSIN_19575"
     misc_feature    complement(2212..2229)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.43"
                     /tool="antismash"
     misc_feature    2234..2248
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.18"
                     /tool="antismash"
     gene            2504..3724
                     /gene="fdhA"
                     /locus_tag="NOSIN_RS19810"
                     /old_locus_tag="NOSIN_19580"
     CDS             2504..3724
                     /EC_number="1.2.1.46"
                     /GO_function="GO:0018467 - formaldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="fdhA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017533154.1"
                     /locus_tag="NOSIN_RS19810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_19580"
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="WP_077692189.1"
                     /sec_met_domain="ADH_N (E-value: 3.6e-27, bitscore: 92.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.8e-08, bitscore:
                     32.1, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGNRGVVYQGAGRVEVQDIAYPEFELKDGPGVNPANVGRKVPHG
                     VILKVVATNICGSDQHMVRGRTTAPEGLVLGHEITGEVVEKGSDVEFVEVGDLVSVPFN
                     ISCGRCRNCKERRTEICLNVNPDRPGSAYGYVDMGGWVGGQAEYALVPYADWNLLRFPD
                     RDRAMEKILDLTMLSDIFPTGYHGCVTAGVGVGSSVYVAGAGPVGLAAAASAQLLGAAV
                     VIVGDMKEERLAQARSFGCEAVNVAEGDPGEQIERILGSPVVDCAVDAVGFEAHGTGKD
                     AEKEAPASVLNTAMALTRAGGSIGIPGLYVTGDPGASDEAAREGSLSIRLGLGWSKSHA
                     FFTGQCPVMKYHRELMAAIMTDRVQIAKAVNAVAIPLEEAPEGYRSFDEGAASKYVLDP
                     NGYLGTG"
     gene            complement(3769..4719)
                     /locus_tag="NOSIN_RS19815"
                     /old_locus_tag="NOSIN_19585"
     CDS             complement(3769..4719)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017563699.1"
                     /locus_tag="NOSIN_RS19815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_19585"
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="WP_077692190.1"
                     /transl_table=11
                     /translation="MANAGPDPDPDSDPATAGPGPAARVDAWLNGAWTLAALAAAAEGR
                     PLSREHGRLLAAAGCAEPDGDGWRLLPAYRALLAGPSGRPAFPRRVARQLGQAADAAVG
                     RDGREEEDDAALLQEGAASGERMGAFLDLLREHVAGFGDLLEKDGLRFLDVGTGVGAVA
                     AAVLDRVPGSSAVGLDVEPRALRLAEHHLAERGLGDRVELRLLDVADLSERGAYDLAWF
                     PLAALAPDTVGRALPRVREALRPGARLLTATVLRDDPADDVLREAVVRWRMSLGRITPW
                     GPREAARELAAAGYRDVRCVRTPERVLTLVTARAP"
     gene            5001..6395
                     /locus_tag="NOSIN_RS19820"
                     /old_locus_tag="NOSIN_19590"
     CDS             5001..6395
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017615044.1"
                     /locus_tag="NOSIN_RS19820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_19590"
                     /product="YcaO-like family protein"
                     /protein_id="WP_077692191.1"
                     /sec_met_domain="YcaO (E-value: 1.8e-39, bitscore: 134.6,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRSSWVPVSGERDGSAALPGPGDRKWSGTRERELPLEEAERRVR
                     AEVRALGWSSLYKVLDDREPTAVQCGLWDADGAEVPYGLGAGKGPGAPARVGAQFEALE
                     HAFTGPTVLESLPLRLVDAADLARGPFAADRAVRDVAAQDGARVACVRHAALDGGPPVD
                     VPVFLWAPWYPLPAGEAAEVRRAVGDTADYGTAMSYSVNSGCAIGATRDEALLHALNEW
                     VERDAFSLFLLGCVYDGGPMPARVPREALPDLPRAHLDRARDLVGGPVVLLDLTTDLGV
                     PVVMAYAPERSGRSPDHRYGLGASLSGELAVERAVTEFVQGELLARVVAEQSASTERTG
                     PFAQLVADHDVATGVGSRFAGHPRLLACAMLDFADRLGEAAPAALPPETVPPGTGVALQ
                     RAAVVERLSGAGHRVAAHPLRTLEHGTTVVQVQCPGLERFHLVTKGLLALPGERGRRLR
                     KRSGAG"
     gene            complement(6542..7564)
                     /locus_tag="NOSIN_RS19825"
                     /old_locus_tag="NOSIN_19595"
     CDS             complement(6542..7564)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019607752.1"
                     /locus_tag="NOSIN_RS19825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_19595"
                     /product="DNA topoisomerase IB"
                     /protein_id="WP_077692192.1"
                     /transl_table=11
                     /translation="MTLRRSDPSEPGITRVRRGRGFRYHGPRGEPVTDPDVIRRIKELV
                     VPPGWHDVWICPFPEGHIQACGTDDAGRRQYVYHREWRRRSEEAKYDRMLDFAEVLPGI
                     RDTVAGHLRRRGLVRERVLAAAVRLVDLGFLRAGGDAYAARNDSYGVASLLREHVHRTG
                     GTVVFEFPGKSGREQRVECAEPEICRVVSGLQRVRSRGQDLFAYRVGDDWHDVTSDDLN
                     SYLRDITGEDHTLKEFRTWHATVLAAVGLAVAVPPDDDKALARLRTHVVREVADYLGNT
                     EAVARSSYIDPRVFRLHERGVTIAPSLPELGREVPAGELATQGEVERTVLRMLREHGDA
                     "
     gene            complement(7615..7917)
                     /locus_tag="NOSIN_RS19830"
                     /old_locus_tag="NOSIN_19600"
     CDS             complement(7615..7917)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017572116.1"
                     /locus_tag="NOSIN_RS19830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_19600"
                     /product="hypothetical protein"
                     /protein_id="WP_077693672.1"
                     /transl_table=11
                     /translation="MAATPPVPGYDDLSVEELRERLLSLSAEQVRELIGYEESHANRMD
                     ALELLEDRLNGLTAGDEIPEGAGPEGGPDPEDEAHAEAVPQSAERPPPDRRTERS"
     misc_feature    7871..7886
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.32"
                     /tool="antismash"
     gene            complement(8073..9038)
                     /gene="rlmB"
                     /locus_tag="NOSIN_RS19835"
                     /old_locus_tag="NOSIN_19605"
     CDS             complement(8073..9038)
                     /GO_function="GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019607750.1"
                     /locus_tag="NOSIN_RS19835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_19605"
                     /product="23S rRNA (guanosine(2251)-2'-O)-methyltransferase
                     RlmB"
                     /protein_id="WP_077692193.1"
                     /transl_table=11
                     /translation="MPAKKNKKGPTKGSGGKNRRSLEAKSGTLPAEQRHWYEGKKRSKA
                     AKRSQPADGQSRGGPGADRRRPDNSDLLVGRNPVVEALRAGMPATRLFLANSLDPDERV
                     NEAARMAGEQGVDVVEVTRTELDRRCESNGQPGAAHQGIVLQVRPYQYRSPEGLLDNAL
                     ESDTPPLIVVLDGVTDPHNLGAIARSSAAFGAHGLLIPERRSAGVNMTTWKTSAGTLAR
                     LPVAQATNLTRALETFKKAGLFVVGLDSEGETPITGLELATGPLVVVVGSEGKGLSRLV
                     RETCDVVASIPIGNAESLNASVAAGVSLYEVARRRAENDG"
     gene            complement(9172..10572)
                     /gene="cysS"
                     /locus_tag="NOSIN_RS19840"
                     /old_locus_tag="NOSIN_19610"
     CDS             complement(9172..10572)
                     /EC_number="6.1.1.16"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cysS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290659.1"
                     /locus_tag="NOSIN_RS19840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_19610"
                     /product="cysteine--tRNA ligase"
                     /protein_id="WP_077692194.1"
                     /transl_table=11
                     /translation="MSLRFYDTSARQVREFVPLREGSASLYLCGATVQAPPHIGHIRSG
                     VNFDILRRWLTHLGYDVTFCRNVTDIDDKIIRVSADEGVPWWQVSERNQRAFTHAYEIL
                     GCLPPTVEPRATGHVPEMIDLIRRLIDAGHAYAAGDGSGDVYFDVSSYPPYGELSNQRP
                     DQVVESADADPGRAKRDPRDFALWKGAKPGEPSWETPWGRGRPGWHLECSAMSTKYLGP
                     SFDIHGGGLDLVFPHHENELAQSRAAGDGFAQYWLHNGMLVVGGEKMSKSLGNSLLIPQ
                     MVRKVRPVELRYYLGHVHYRSAIDYSDASLQEAATAYQRVEGFLIRAVEVLGEIAPAAD
                     VPAEFASALNDDLGVSQALAVVHGHVREGNTALTSGGKEQVAEIAGELRTMLDVLGLDP
                     LSEQWAEGDQGLREVVDALVAVALEQRQAARGRKDYAAADAIRDQLTEAGIVVEDTPQG
                     PRWDLRRS"
     misc_feature    10651..10668
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     misc_feature    10822..10836
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.49"
                     /tool="antismash"
     misc_feature    complement(10867..10883)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.83"
                     /tool="antismash"
     misc_feature    complement(10903..10917)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.09"
                     /tool="antismash"
ORIGIN
        1 cagtccacgt cctcatcctc tatgaccgct cggtgatgga gcggtaaccc aggtatgacg
       61 gtgtgccgct tcccaggaca cggcccgcga aacctcccga agaccggagg gggaggaccg
      121 gccccgggga aggcgtcgac ccgtcgccgg gcccggggaa gggaaggccg gacgtcggcg
      181 cgcggtggca ggatgcccgg atggggaacc tctaccagta cttccacgct ccgacgacag
      241 ggcggtactg atcgcgtccg gaccccgtgc ccactcggcc acgtccccgt ggcaccgcgg
      301 ttctggccga acacggtcgt ccgccgctgg accacgtcac cacggaccgc gttcacaccc
      361 atctaccccg tgccatggtg atcggagaag acccagcgca ggctgtcgtt gctctcccgg
      421 ccgcaaccgt ctcgcccgcg tgctcgaagt aacgggtagc ctccccggtt cccgccgcca
      481 ggcctctcag caatcccggc gcggaggcga tgtgggtggt cacgctgtac tgaccatgga
      541 ggttggtaac acccaccccg gctgacacac acgaagaggg ccttcggtat cggtgtggat
      601 tgcgagatct acccgactca ccagaaggcc ctcgatgccc cacactaccc acgccaacgc
      661 tcgtctgact cccgcaggac gcctcgcgtt ggcccgctgc gtcatcgacc aggggtggcc
      721 gctgcggcgt gccgccgaac gcttccagac cagcaccacc accgccaaac gctgggccga
      781 ccgctaccgg gccacgggtg aagcgggcat ggtcgatgcc tccagccgcc cggcccgctc
      841 accacggcgc acccggcccc gacgcgagcg ccgcgtgatc aagctgcgcc acctgcgccg
      901 gtggggcccg gcccgtatcg gcggccacct gggcatgcac gcctcgacgg tgcaccggat
      961 cctgacccgc tacgggtgtg cccgcctggc ccacaccgac cgggccaccg gacgggcggt
     1021 gcgccgctac gagcgcgaac gccccgggga gctggtgcac gtggacatca agaagctcgg
     1081 caacatcccc cacggcggcg gccacaaggc gctgggacgc ccccgggggc gcaggcaccg
     1141 ctccaacgcg gggtatgcct acctgcacaa cgccgtggac gaccactccc ggttggccta
     1201 ctccgagatc ctgaccgatg agaagaagga gaccgccgtc gcgttctggg agcgtgcgca
     1261 cgcctacttc gcgtcggtgg ggatcacggt ggagcgggta ctgaccgaca acgggtcctg
     1321 ctaccgctcg cgtctgtggc gtgacctggt ccgtgaccag ggcgtcaagc acaagcgcac
     1381 ccgtccctac cggccccaga ccaacggcaa ggtggagcgc ttcaaccgga tcctggtcca
     1441 ggagtgggcc tacgcccgcc cctatgcgtc agagtcccaa cggcgggagg cgttcccggg
     1501 gtggttgcat cactacaatc accaccggtt ccacaccgcg ctcggtggcc ctcccgcctc
     1561 ccgcgtcacc aacctctcag ggcagtacag ctaggtcgcg gcgcgcgaga aaatccctac
     1621 cctcggccgt gctgaggatc ctgatgatct cgtcctgctc gaagaccggg accggctccg
     1681 gtgtcacctt gggtggcttc atcttgcgca tggggttggc gatctcctcc tcgtccgtca
     1741 accacttgaa gaactgctgc aggcaccggt actggttgtt gacgtaggcg tccttgtgct
     1801 gacggtggcg cagccgcacc agccaggagc gcaggtgcac acgggagacc agctcccagt
     1861 cgcgcacggg ctcgaagcgc agcatgtcag cgctggtgtc agcgtcggtg tcgaagcggg
     1921 tgtcggtcgg tgggctgacc gccggtcagc aggtgctcgg cagcgaacca gcgcacggcg
     1981 tcggcgtaca tcgtgacggt gttgtcgcgt ttgccctcgg agcgcaggtg gagcacgaag
     2041 gagtcgacgt gggtcctcag gggccccgcg gccaggttgc gcgggcctcg acggatacga
     2101 ccggtagcca taagcgatac ctcgtcaagc tctccagcct atggaggctg tcctgcttca
     2161 cgagttcgcc caggtcagac catgtgagag ccagcgaggg gacttgaacc cctaacctat
     2221 cgcttacaag gcgattgctc tgccaattga gctacgctgg cgggctgccg agaccgtggc
     2281 cccggttgcg tcccggggaa atggtaccgg gtcagaggcc accaggccta ctcggatgcc
     2341 tccgagccgc gcggacgcca tcccgtccgt cggccctcgg ccgcgtccct ccccgatccg
     2401 gcaaagaagc acctggcggc gtgcggagcc cggttagccg ccgtcccgtc cggaaacgac
     2461 agggggacat gctccctccc gggggcggag aggtggaagg gccatggcgg gcaacagggg
     2521 cgtggtgtac cagggcgcgg gcagggtcga ggtccaggac atcgcctatc cggagttcga
     2581 actcaaggac ggcccggggg tcaacccggc gaacgtgggg cgcaaggtgc cgcacggggt
     2641 gatcctcaag gtcgtcgcca ccaacatctg cggaagcgac cagcacatgg tgcgggggcg
     2701 caccaccgcg ccggaggggc tggtcctggg ccacgagatc accggggagg tggtcgagaa
     2761 ggggtccgac gtcgagttcg tcgaggtcgg cgacctggtc tcggtaccgt tcaacatctc
     2821 ctgcgggcgt tgtcggaact gcaaggagcg gcggacggag atctgcctca acgtcaaccc
     2881 cgaccgcccg ggctcggcct acggctacgt cgacatgggc ggctgggtcg gggggcaggc
     2941 cgagtacgcg ctggtgccct acgccgactg gaacctgttg aggttccccg accgcgaccg
     3001 ggcgatggag aagatcctcg acctgacgat gctctccgac atcttcccga ccggctacca
     3061 cggctgcgtc accgcgggcg tgggcgtggg gtcgagcgtc tacgtcgcgg gcgccgggcc
     3121 cgtcgggctt gccgcggccg cgtcggccca actgctgggc gcggcggtgg tgatcgtcgg
     3181 tgacatgaag gaggagcgcc tggcccaggc ccgcagcttc gggtgcgagg cggtcaacgt
     3241 ggcggagggc gaccccgggg agcagatcga gcggatcctg ggcagccccg tggtggactg
     3301 cgcggtggac gccgtgggct tcgaggcgca cgggaccggg aaggacgcag agaaggaggc
     3361 gcccgcgagc gtgctgaaca ccgcgatggc cctgaccagg gcgggaggct cgatcggcat
     3421 ccccggcctg tacgtgaccg gtgaccccgg cgcctccgac gaggccgccc gcgagggctc
     3481 cctgtcgatc cggctcgggc tgggctggtc caagtcgcac gccttcttca ccggccagtg
     3541 cccggtcatg aagtaccacc gggaactgat ggcggcgatc atgaccgacc gtgtgcagat
     3601 cgccaaggcc gtcaacgccg tggcgatccc gctggaggag gccccggagg gctaccggtc
     3661 cttcgacgag ggcgcggcca gcaagtacgt gctcgacccg aacggctacc tcggcacggg
     3721 ctgagacccg cgggagggga accggccggg gaggccgcgc ggcgggggtc acggggcgcg
     3781 ggcggtgacg agggtgagga cccgctcggg cgtccgcacg caccgcacgt cccggtagcc
     3841 cgccgccgcc agttcacgcg ccgcctcccg cggtccccac ggggtgatcc ggccgaggga
     3901 catgcgccag cgcacgaccg cctcgcgcag cacgtcgtcg gccgggtcgt cgcgcagcac
     3961 ggtcgcggtg agcaggcgcg cgcccggccg cagggcctcg cgcacgcggg gcagcgcccg
     4021 cccgacggtg tcgggtgcga gggccgccag ggggaaccag gccaggtcgt aggcaccccg
     4081 ctcggagagg tcggccacgt ccagcaggcg cagttcgacc cggtcgccca gcccgcgctc
     4141 ggccaggtgg tgctcggcca ggcgcagagc ccggggttcg acgtccaggc cgaccgcgct
     4201 ggagccggga acccggtcga gcacggcggc cgcgacggcg ccgaccccgg tccccacgtc
     4261 gaggaagcgc aggccgtcct tctccaggag gtctccgaat cccgcgacgt gctcccgcag
     4321 caggtcgagg aaggccccca tccgctcgcc ggaggcggcg ccctcctgga ggagggccgc
     4381 gtcgtcctcc tcttcacggc cgtcccggcc cacggcggcg tcggcggcct ggcccagctg
     4441 ccgcgcgacc ctgcggggga aggccggcct gccggaggga ccggccagga gggcgcggta
     4501 ggccgggagg agccgccacc cgtcaccgtc gggttcggcg caccccgcgg cggcgagcag
     4561 cctgccgtgc tcccgcgaca gcggtcggcc ctccgcggcg gcggcgagcg ccgccagcgt
     4621 ccaggcgccg ttgagccacg cgtcgacacg ggccgcgggg ccgggtccgg ctgtcgcggg
     4681 atcggagtcg gggtcggggt cgggtccggc gttggccatg gcgtgagtgt gccaccctcc
     4741 gcgccccgac gcactcccag gttttgagac ccggtcccac ataaggcaga acgtcagttt
     4801 tccgcgcttt ttcagtgaca ggcgtcacca tgttggggta aagagtacgg gagcgcgtac
     4861 acaacgtcgc gttccatgtg tctaccgctg aggagtcacg acatggcctg cgctggttac
     4921 gagtggaccg ttccgctgga gaacgcggac gacatcgccc ccgccgtcga tgagctggac
     4981 gaggagaccg cggagtagcg gtgcctcgat cctcgtgggt ccccgtgtcc ggggagcggg
     5041 acggctcggc cgcgctcccc ggaccgggcg accggaagtg gtccggtacg cgcgagcgcg
     5101 agcttcccct ggaggaggcg gagcggcggg tccgcgccga ggtgcgcgcc ctcggctgga
     5161 gttcgctgta caaggtgctc gacgaccggg agccgaccgc cgtccagtgc ggcctgtggg
     5221 acgcggacgg cgcggaggtc ccctacgggc tcggcgcggg caagggcccg ggcgcccccg
     5281 cgcgcgtcgg cgcgcagttc gaggcccttg agcacgcgtt caccggtccc accgtcctgg
     5341 agtcccttcc gctgcggctg gtcgacgccg ccgacctggc ccggggcccc ttcgccgccg
     5401 accgcgcggt ccgcgacgtg gccgcccagg acggcgcccg ggtggcctgc gtgcgccacg
     5461 ccgcgctcga cggcggacca cccgtcgacg tccccgtgtt cctgtgggcg ccctggtacc
     5521 cgctgcccgc cggggaggcg gcggaggtgc gccgtgccgt cggcgacacc gccgactacg
     5581 gaacggcgat gtcctacagc gtcaactccg gctgcgccat cggcgccacc cgggacgagg
     5641 cgctcctgca cgccctcaac gagtgggtgg agcgcgacgc gttctcgctg ttcctcctgg
     5701 gctgcgtcta cgacggcggt cccatgcccg cgcgcgtgcc ccgcgaggcc ctgcccgacc
     5761 tcccgcgcgc ccacctggac cgcgcccgcg acctcgtcgg cggccccgtg gtcctgctcg
     5821 acctgaccac cgacctcggt gtccccgtgg tcatggccta cgcccccgag cggtcgggga
     5881 ggagcccgga ccaccgctac gggctcggcg cctccctctc gggcgaactc gccgtggagc
     5941 gcgccgtcac cgagttcgtc cagggcgagc tgctcgcccg ggtcgtggcc gagcagtcgg
     6001 cctcgacgga acgcaccggg cccttcgccc agctcgtcgc ggaccacgac gtcgccaccg
     6061 gggtcgggtc ccggttcgcc gggcatcccc ggctcctggc ctgcgccatg ctggacttcg
     6121 ccgaccgcct cggcgaggcc gccccggccg cgcttcctcc cgagaccgtt ccgccgggca
     6181 ccggcgtggc cctccagcgg gccgcggtcg tggaacggct ctccggggcc ggtcaccgcg
     6241 tcgcggccca tccgctccga accctggagc acggaacgac ggtcgtgcag gtccagtgcc
     6301 ccggcctgga gcggttccac ctggtcacca agggcctcct ggcccttccc ggcgaacggg
     6361 gacgaaggct ccggaagcgg agcggggcgg ggtgaggcgg cgccttcggc cgccctcggc
     6421 cagtgccggc ccggccggtc ccaccggtcc ccgtcggtcc cgaccggtac cgcggcacgc
     6481 cgcggcgccg tggacgggac cggctcccgg gtccggaacc ggcgcccggg gccggtgcgg
     6541 gtcaggcgtc gccgtgctcg cgcagcatgc gcagcaccgt cctctccacc tcgccctggg
     6601 tggcgagctc gcccgcgggc acctccctgc ccagctccgg tagcgacggg gcgatcgtca
     6661 ccccgcgttc gtgcagccgg aagacccgcg ggtcgatgta ggagctccgg gccacggcct
     6721 cggtgttgcc caggtagtcg gccacctcgc ggaccacgtg ggtcctcagg cgcgcgaggg
     6781 ccttgtcgtc gtcggggggc acggccaccg cgagtcccac cgcggccagc accgtcgcgt
     6841 gccaggtccg gaactccttg agcgtgtggt cctccccggt gatgtcccgc aggtaggagt
     6901 tgaggtcgtc gctggtcacg tcgtgccagt cgtccccgac gcggtaggcg aacaggtcct
     6961 gcccccggga gcggacgcgc tggagcccgg agaccacccg gcagatctcg ggttcggcgc
     7021 actcgacgcg ctgctccctg cccgacttgc ccgggaactc gaacacgacc gtgccgccgg
     7081 tgcggtggac gtgctcgcgc agcaggctcg ccaccccgta ggagtcgttc cgggcggcgt
     7141 aggcgtctcc gcccgccctc aggaagccca ggtcgaccag ccggacggcc gccgccagaa
     7201 cgcgctcgcg caccagtccg cgacggcgca ggtgcccggc caccgtgtcc ctgatccccg
     7261 ggagcacctc ggcgaagtcg agcatcctgt cgtacttggc ctcctcgctc ctgcgccgcc
     7321 actcccggtg gtagacgtac tggcgccggc ccgcgtcgtc ggttccgcac gcctggatgt
     7381 gcccctccgg aaaggggcag atccacacgt cgtgccagcc gggcggcacc acgagttcct
     7441 tgatgcgccg gatcacgtcg ggatcggtga ccggctctcc ccgggggccg tggtagcgga
     7501 aaccgcggcc gcgccggacc cgggtgatcc cgggctcgga cgggtcactg cgccgtagcg
     7561 tcataccgcg tccccgtcat ggacatccat gggacctcct gagacggacg ccggtcagga
     7621 ccgctccgtc cgacggtcgg gcggcgggcg ctccgcgctc tggggcaccg cctccgcgtg
     7681 ggcctcgtcc tcggggtccg ggccgccctc gggcccggct ccctcgggga tctcgtcccc
     7741 cgccgtgagc ccgttcaggc ggtcctcgag gagctccagc gcgtccatgc ggttggcgtg
     7801 gctctcctcg tacccgatca gttcgcgtac ctgttccgcc gacagggaga gaaggcgttc
     7861 ccgcaactcc tcgaccgaca ggtcgtcgta tccggggacg ggaggtgtcg ccgccatgtc
     7921 gctcacctgt gtttcctctt cgggcggtac ggacgtagcc gcacagctac ccgtacgccg
     7981 ggaggaaaaa caggggcggc gcaccgcacg gtgcgccgac cagcggggcc cgaggccccc
     8041 cggtcacagc ggcggcgcct tcggccaggc cgtcagccgt cgttctcggc tcggcggcgg
     8101 gccacctcgt agagggacac cccggcggcc acggaggcgt tcagcgactc cgcgttgccg
     8161 atcgggatgc tcgcgaccac gtcacaggtc tcgcgcacca ggcgggacag gcccttgccc
     8221 tccgagccca ccacgaccac cagcgggccg gtggccagct ccaggcccgt gatcggggtc
     8281 tcgccctcgg agtcgaggcc gacgacgaac aggcccgcct tcttgaaggt ctccagggcc
     8341 cgggtcaggt tggtggcctg ggccacgggc agccgggcca gggtgcccgc ggaggtcttc
     8401 caggtggtca tgttgacccc cgccgagcgg cgctcgggga tgaggaggcc gtgcgcgccg
     8461 aacgcggcgg acgaacgcgc gatggcgccc aggttgtgcg ggtcggtgac cccgtccagg
     8521 acgacgatca gcggcggggt gtcggactcc agcgcgttgt ccagcaggcc ctcgggggac
     8581 cggtactggt agggccgcac ctggagcacg atcccctggt gggcggcgcc gggctgcccg
     8641 ttgctctcgc agcggcggtc gagctcggta cgggtcacct cgaccacgtc cacgccctgc
     8701 tcacccgcca tccgggcggc ctcgttgacc cgctcgtcgg ggtccaggct gttggccagg
     8761 aacaggcgcg tggcgggcat ccccgcacgc agcgcctcca ccaccgggtt gcgtccgacc
     8821 agcaggtcgg agttgtccgg acggcggcgg tcggcacccg ggccgccccg cgactgaccg
     8881 tcggcgggct gggagcgctt ggcggccttg gaccgcttct tgccctcgta ccagtgccgc
     8941 tgctcggcgg gcagggtgcc gctcttggcc tccagggaac ggcggttctt gccgccgctg
     9001 cccttggtcg ggcccttctt gttcttcttc gccggcatgg tcgccgcctc cccgtacgtg
     9061 ttgcgaacat gccggaggcg cggggcatcc ggctggtgga atatcgccga ccggtgcgaa
     9121 cacggcggca aactcccagc ctagtgccgc cgtgtcaccg cacgcgtcgc ctcagcttcg
     9181 gcgcaggtcc cagcgcgggc cctgcggggt gtcctcgacc acgatgcccg cctcggtgag
     9241 ctggtcgcgg atcgcgtcgg ccgccgcgta gtccttgcgc ccgcgggccg cctggcgctg
     9301 ctccagggcg accgccacca gggcgtcgac gacctcgcgc agcccctggt cgccctcggc
     9361 ccactgctcg ctgagcgggt ccaggccgag gacgtcgagc atggtgcgca gctcaccggc
     9421 gatctcggcg acctgctcct tgccccccga ggtcagcgcc gtgttgccct cgcggacgtg
     9481 cccgtgcacg acggcgagag cctgggagac cccgaggtcg tcgttgagcg cggacgcgaa
     9541 ctcggcgggc acgtcggcgg cgggggcgat ctcgcccagc acctcgacgg cccggatcag
     9601 gaagccctcg acgcgctggt aggcggtggc cgcctcctgg agcgacgcgt cggagtagtc
     9661 gatggcggag cggtagtgca cgtggccgag gtagtagcgc agctcgacgg gacggacctt
     9721 gcgcaccatc tgcgggatga gcagggagtt gcccagcgac ttgctcatct tctcgccgcc
     9781 cacgacgagc atcccgttgt gcagccagta ctgggcgaag ccgtcaccgg cggcgcggga
     9841 ctgggcgagc tcgttctcgt ggtgcgggaa gaccaggtcc aggccgccgc cgtggatgtc
     9901 gaagctgggg cccaggtact tggtggacat ggccgagcac tccaggtgcc agccggggcg
     9961 gccgcgtccc cacggggttt cccagctggg ctcccccggc ttggcgccct tccagagggc
    10021 gaagtcgcgg gggtcgcgct tggcccggcc cgggtcggcg tcggccgact ccacgacctg
    10081 gtcggggcgc tggttggaca gctccccgta gggcgggtag gagctcacgt cgaagtagac
    10141 gtcgccggag ccgtcacccg cggcgtaggc gtggcccgcg tcgatgaggc ggcgtatgag
    10201 gtcgatcatc tcgggcacgt gtccggtggc ccgcggctcg acggtgggcg gcaggcagcc
    10261 caggatctcg taggcgtggg tgaaggcgcg ctggttgcgc tcgctgacct gccaccaggg
    10321 cacgccctcg tccgccgaca cccggatgat cttgtcgtcg atgtcggtga cgttgcggca
    10381 gaaggtgacg tcgtacccca ggtgcgtcag ccagcggcgc aggatgtcga agttcacccc
    10441 ggaccggatg tgtccgatgt gcgggggcgc ctgcaccgtg gcaccacaca ggtacaggga
    10501 ggcggatccc tcacgcaggg ggacgaactc gcggacctgt cgggcactgg tgtcatagaa
    10561 gcgcagactc acgttgtcaa gcgtagccgc tttcccctcc cgcggacgtc acagatcagg
    10621 aagcacggcc cccgtggaag tcttcacgga catccccaag tgcgtatatg tgattagccg
    10681 tattccggca gccgtggcgt gagacagctc caccacaaag cccgtctcct tccatcgccg
    10741 agccactatc cactgcatat cccgacagaa cggcacagaa cgccttcagg acggctcgtc
    10801 tggaagcggt cataatcatc cggaatctcg tgttcgcggc accgcctcga ctacgctgcg
    10861 gaggcgatgg ccccttacaa caacgccccc gaggcaggga cacgggacgg tcggcccacg
    10921 gcaggtcgcg gttcgggagc cggtgctccc gtcggtggcg ccctgttcgt cggcctcatc
    10981 ggcctgtgcg ccctcgtcat ctcctacaac ggcatcttcc cgctcgccga gtacggcgga
    11041 cacgaggacg ccctcctcgc ccacgcgttc ccggtggcct acacgctgct gctggtcatg
    11101 gcgtgctggg tgagctacct cctgcgcgac gcgcacccgc gtgagcggtt ctgggtcgac
    11161 ctggtgctga tcccgctcct ggtcctgttc gcgaccgtgg cgatgctgct gcacaacctc
    11221 cagctcatcg agcgggtgca ccagggggtc gccaacgtgg tcgtggcggt gatgccgttc
    11281 gcgggcctgc tggtggcgtt cctgctgtgg atggtcctgc gcgcgcacac gcgcaggcgg
    11341 cgccgccgcg ggccggtcgc acggccgcgc ccggcggacg accccaccac ggtgc
//
