LOCUS       NZ_MCOK01000001        10732 bp    DNA     linear   CON 22-OCT-2024
DEFINITION  Nocardiopsis sinuspersici strain UTMC 00102 NOSIN_1, whole genome
            shotgun sequence.
ACCESSION   NZ_MCOK01000001
VERSION     NZ_MCOK01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sinuspersici
  ORGANISM  Nocardiopsis sinuspersici
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 6071583)
  AUTHORS   Tokovenko,B. and Kalinowski,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2016) Helmholtz Institute for Pharmaceutical
            Research Saarland, Helmholtz Centre for Infection Research, Campus
            C2.3, Saarbrucken 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2016
            Assembly Method                   :: SPAdes v. 3.8.1
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina NextSeq; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001998325.1-RS_2024_10_22
            Annotation Date                   :: 10/22/2024 13:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,351
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 5,224
            CDSs (with protein)               :: 5,224
            Genes (RNA)                       :: 77
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 13 of 50
            Pseudo Genes (incomplete)         :: 44 of 50
            Pseudo Genes (internal stop)      :: 2 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:06:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2402670
            Orig. end                         :: 2413402
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCOK01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10732
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="4"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10732
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10732
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5732](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5732)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    623..637
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            980..2332
                     /gene="paaK"
                     /locus_tag="NOSIN_RS10360"
                     /old_locus_tag="NOSIN_10225"
     CDS             980..2332
                     /EC_number="6.2.1.30"
                     /GO_function="GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (42-352). E-value: 7.5e-22.
                     Score: 69.4. Matches aSDomain:
                     nrpspksdomains_NOSIN_RS10360_AMP-binding.1"
                     /codon_start=1
                     /gene="paaK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910203.1"
                     /locus_tag="NOSIN_RS10360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_10225"
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="WP_077690532.1"
                     /sec_met_domain="AMP-binding (E-value: 2.3e-23, bitscore:
                     80.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASHDTTVRTAGTAPRLGQAPSPELLDPAERMSVDELRALQLKRL
                     RWTLEHAYANVPLYRKKFDEAGVRPEDLRELEDLRHFPHTTKADLRDNYPFGMFAVPQE
                     RVSRIHASSGTTGRPTVVGYTREDIDVWAEVVARSIRASGGRAGHKVHVSYGYGLFTGG
                     LGAHYGAERLGCTVIPASGGQTAKQVQLIQDFEPDVIMITPSYMLTLLDEFEHQGIDPR
                     STSLKVGVFGAEPWTEKMRTEIEERFDIHAVDIYGLSEVMGPGVANECVETKDGLHVWE
                     DHFYPEVLDPLTQDVLPDGSNGELVFTSLTKQAMPVIRYRTRDLTTLRPGTARPMRRMD
                     KVTGRSDDMIILRGVNVFPTQIEEIVLGLDGLAPHFQLVLTKEGRLDRMAVRIEAAPDC
                     TPERRVALSREIVATVKDKVGVSVTVDVVDPEQIERSVGKFRRIVDQRPKE"
     aSDomain        1106..2035
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NOSIN_RS10360_AMP-binding.1"
                     /evalue="7.50E-22"
                     /label="NOSIN_RS10360_AMP-binding.1"
                     /locus_tag="NOSIN_RS10360"
                     /protein_end="352"
                     /protein_start="42"
                     /score="69.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="KRLRWTLEHAYANVPLYRKKFDEAGVRPEDLRELEDLRHFPHTTK
                     ADLRDNYPFGMFAVPQERVSRIHASSGTTGRPTVVGYTREDIDVWAEVVARSIRASGGR
                     AGHKVHVSYGYGLFTGGLGAHYGAERLGCTVIPASGGQTAKQVQLIQDFEPDVIMITPS
                     YMLTLLDEFEHQGIDPRSTSLKVGVFGAEPWTEKMRTEIEERFDIHAVDIYGLSEVMGP
                     GVANECVETKDGLHVWEDHFYPEVLDPLTQDVLPDGSNGELVFTSLTKQAMPVIRYRTR
                     DLTTLRPGTARPMRRMDKVTGRSDDMIIL"
     CDS_motif       2006..2068
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NOSIN_RS10360_0001"
                     /evalue="2.00E-04"
                     /label="NRPS-A_a8"
                     /locus_tag="NOSIN_RS10360"
                     /protein_end="363"
                     /protein_start="342"
                     /score="14.1"
                     /tool="antismash"
                     /translation="TGRSDDMIILRGVNVFPTQIE"
     gene            complement(2411..3418)
                     /locus_tag="NOSIN_RS10365"
                     /old_locus_tag="NOSIN_10230"
     CDS             complement(2411..3418)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570554.1"
                     /locus_tag="NOSIN_RS10365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_10230"
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="WP_077690533.1"
                     /sec_met_domain="fabH (E-value: 3.6e-92, bitscore: 307.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEDTKVRESVPIGVIGTGSRLPERQVGNTEVGAPAGVDDAWIVRK
                     TGIRHRRWADPGEATSDLAADAGRAALERAGISPADLSFVVVATSTPDSPQPPTATAVA
                     EMVGATPDTAAFDLNAVCSGFVFALSVVQRALSASGGGYALVIGADVYSRILDPADRRT
                     VVLFGDGAGAAVLGPVPRGRGLLATRLSSSGAHRDLIRVPAGGSRRPASEATLAGGEHY
                     FRMDGRGVRDFVSEHVPPAVRAFLKDSGVGEADIAHLVPHQANGHMVDGLARGLGLPVA
                     RVHTTVREYGNTGAASIPVTLDAAVRSGAVHPGDTVLLTGFGGGMASGFALMRW"
     misc_feature    complement(3356..3358)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3516..4082)
                     /locus_tag="NOSIN_RS10370"
                     /old_locus_tag="NOSIN_10235"
     CDS             complement(3516..4082)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021453.5"
                     /locus_tag="NOSIN_RS10370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_10235"
                     /product="DUF2165 family protein"
                     /protein_id="WP_077690534.1"
                     /transl_table=11
                     /translation="MMVQTRRASRTGPRSAREDATGLLYPQTLVLGGMAAWLSLIMFNN
                     ITDSGTNITLIGQVLTMEGIIADPDRGNGLVGRALPGSLAAPALYAVIAYQALSVLLLW
                     TATVTHTRLLLGRGDGTRATFLGNIALCSLAGLALMFLVGGLWFGYWIALPPVQQVHFT
                     LLIIAIGAMVLINMPRTVRPADGVT"
     gene            complement(4079..4930)
                     /locus_tag="NOSIN_RS10375"
                     /old_locus_tag="NOSIN_10240"
     CDS             complement(4079..4930)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570552.1"
                     /locus_tag="NOSIN_RS10375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_10240"
                     /product="HAD family phosphatase"
                     /protein_id="WP_236743341.1"
                     /transl_table=11
                     /translation="MSESERRYGPRYGAFFDVDETLISGKSMFSFLAFYLRERDEPPSV
                     YERMAAELRRRARKGAPREAVNRAYYRFYRGEPRDRIAASGRRWFAERAADPDFFHAGA
                     VRELDRHRTAGADVVLLSGSFFACLDPVAEALGATVALGSEPLVRGGLLTGEVGRTMIG
                     RAKGEAAAEFAASRGLSLPDSHGYGDHISDLALLEAVGHPHVVEGDPALREHAIARGWP
                     VLDAPSPSPRARGGARSTRRTGRHGDECCCGCALASWSAGTDTHTHTASEPAGRPTETG
                     VR"
     gene            complement(5001..5732)
                     /locus_tag="NOSIN_RS10380"
                     /old_locus_tag="NOSIN_10245"
     CDS             complement(5001..5732)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.5"
                     /locus_tag="NOSIN_RS10380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_10245"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_170293709.1"
                     /sec_met_domain="AfsA (E-value: 1.1e-12, bitscore: 46.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHERVQALSSFVGGLRAGVYDDIRGPLVLRGGQGIGGYEWDYIRG
                     ALRRRGLEERVVLHDRPPVLAERAEAHKHREENVLIADLGRHDDQVFHAALRLHGDNEL
                     LLDHQTGQHTQGMVVVEAARQMFLAVTERYYTHRWPERRYYFVINSLATTFENFLFPVD
                     ARIRYLIQSAELSDPGRLEFTARVDIEQGGRRSARCEVAFTAFDTAVLEAVEYRRAGRA
                     IDHVLADTDVREAEHAASSLV"
     misc_feature    5780..5794
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.28"
                     /tool="antismash"
     misc_feature    complement(5870..5883)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.82"
                     /tool="antismash"
     gene            complement(6042..7184)
                     /locus_tag="NOSIN_RS10385"
                     /old_locus_tag="NOSIN_10250"
     CDS             complement(6042..7184)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584351.1"
                     /locus_tag="NOSIN_RS10385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_10250"
                     /product="MFS transporter"
                     /protein_id="WP_236743342.1"
                     /transl_table=11
                     /translation="MTTIAGLSLAGILVVGQLYTVIPLFSLMAGDWGSSPTAVAWTATA
                     FAIAYAVGFLFAGPAADRFGPRRTIVVGLAATAVVTIAFPLVSSLAAALVLRVVQGLVA
                     AAFAPSAFAYIATRIDPHRRVAAITWLTSSFLAAAVLGQLAAQAIGQAAGWEPVFLAGG
                     VALALGSVGLRLVLRADEADGRATVADAFAAMAGLLGNRRLLLLYGTTLMVLLGFVGVY
                     SGLQVAGPPSLAGNDGALLILRASGLPAMLAIPLITPWLARTAPERRVAIALGVAALLS
                     VVTGFLTDGAAIGVCLFVFVAGIAVAAPGLVQAIGAQAGAARGAAVALYTFFLFVGAGL
                     GPQLATALAGFGFPAVSLAAAGILGAGALLALGAATRVRP"
     misc_feature    complement(7337..7349)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.22"
                     /tool="antismash"
     gene            7403..8020
                     /locus_tag="NOSIN_RS10390"
                     /old_locus_tag="NOSIN_10255"
     CDS             7403..8020
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624812.1"
                     /locus_tag="NOSIN_RS10390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_10255"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_077690538.1"
                     /transl_table=11
                     /translation="MTKRTEITRALLLRAARTEFAAYGIAGARVDRIAERAGVNKQRLY
                     ANFGDKEQLFQHVVSDALDELSQVVALDDGTDPAAYVARVFDYHRTKPELLRLLLWESL
                     HYGEKPLPNEEDRTALYEAKLSSLAERTGTPLSRDTRHLLLSLIGLAAWPQIVPQLARL
                     IVGPDIHEDEAQARLRDHIIAFVRNATETAPSVPDTGTDGAL"
     gene            complement(8082..8897)
                     /locus_tag="NOSIN_RS10395"
                     /old_locus_tag="NOSIN_10260"
     CDS             complement(8082..8897)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584353.1"
                     /locus_tag="NOSIN_RS10395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_10260"
                     /product="hypothetical protein"
                     /protein_id="WP_227014843.1"
                     /transl_table=11
                     /translation="MATRTRTAGSARTRSPVAGVLAMALAAVLGSSIWAYAAGESEPGP
                     GPTDHALDITEEDGMDETVAAADENGGGGVRSARLEEAGLAVPSGWHEAGVRTGSHDGE
                     KVTVTRYQPTEEVRLGGPHVSVVRGEDGRVVGYTALDPDAHNDPRDLPGAEQARQAAYS
                     WLGRTDDDYLRGLTEQWVDRHDETVVDEDGREHVIPGVKVKTRHEDGRYAWVIVGADSR
                     VVTFERDVTWDTAARRRATQMWLHDTWIAALEGGGEQPPPPAALADGAA"
     gene            9223..10068
                     /locus_tag="NOSIN_RS10400"
                     /old_locus_tag="NOSIN_10265"
     CDS             9223..10068
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1260: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017568753.1"
                     /locus_tag="NOSIN_RS10400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_10265"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_236743343.1"
                     /transl_table=11
                     /translation="MPTPDIRPGPAAGPRAAGPSRDGAVDSRRLPLYAAGEIDVPFVVR
                     GSAEIIPRDTFWEEHSHPTHELLWNQRGASSATIGARVWTITPAVGLWLPAGTRHSGWT
                     PAGTWHRAAQFGVHAVPPISDGPVAMEVTPLLRLLLDRLDADDLTRDARSRTEAMVLDL
                     LTPARHELILRIPESPLLAPIAAAVREDPADATTLAVWAARLGVSTRTITRAFRSETGL
                     GFSQWLATARMQHAVAMLAREEEIDEVAACVGYHSASAFGAAFRRITGLSPGRFRSEIQ
                     "
     misc_feature    10173..10191
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: medium, score: 28.73"
                     /tool="antismash"
     misc_feature    complement(10178..10196)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.56"
                     /tool="antismash"
ORIGIN
        1 ctctccatgg tcgagtccgg gcgcgggccc tcgtcggcgg tgacctcgtg ggtccggccc
       61 ttgcggccgt gcacggtgac cgggacggtc tcggcgtcga agcggcccgc ctcggcggcc
      121 gcgaccgcgc gctcgtggct gcgcagggcg aaggcgtcgc actcctgccg ggtgaggccc
      181 tccaggcgcg cgacctcctc ggccgtctcc cccatcgaca gggtgtagcg cagctcgtcg
      241 ggggaggcgt cggccggagc ctgggcgtcg gccttcgcga aacgcgggtt ggtgaagcgc
      301 catcccaggg aggtgtcggc gacctcgccc ggcttggccc agggggtgcc gggcttggcc
      361 atcacccagg gggcgcgggt catggactcc acgccacccg cgaccaccac gtcgacctcg
      421 cccgagcgga tggcctgggc ggccgaggac acggcggtca ggccgctggc gcacagccgg
      481 ttgacggtgt agccgggaac gctcgggtcg agcccggcca gcagcaccgc catgcgggcc
      541 acgttgcggt tgtcctcacc ggcctggttg gccgcgccga ggatgacctc gtccacgacg
      601 tcgccgggaa cgcccgcacg gcggaccacc tcggccacgg ccgtggcggc caggtcgtcg
      661 gggcgcactg ctgccagggc accgccgtag cggccctggg gggttcgcac cccgtcgacc
      721 aagtagacct cgttcacgtg cggcctccca tgttaacgac ggtggtccgg gccaccgcca
      781 cctgtgaaat catcgacact tcgtccttga caccgctgtc cggagactgc gttaatgcat
      841 tagtaaccga acgttcggtc agtaagcaag ccggtccgct cctcgacgga ccgcacccac
      901 ccgcgacccg cgcacacgcc cgcccgcctc ccccaggacc gctgtcggcg ggtctcgcgc
      961 acagtacatg gagaggccca tggcaagcca cgacaccacc gtgcggacgg ccggcacggc
     1021 cccccgcctg gggcaggccc cctcgcccga actcctggac ccggccgagc gcatgagtgt
     1081 cgacgaactg cgcgccctgc agctcaagcg cctccgatgg accctggagc acgcctacgc
     1141 caacgtgccc ctgtaccgga agaagttcga cgaggccggg gtgcggcccg aggacctcag
     1201 ggagctcgag gacctgcgcc acttcccgca caccaccaag gccgacctgc gcgacaacta
     1261 ccccttcggg atgttcgccg tcccccagga gagggtcagc cgcatccacg cctccagcgg
     1321 gaccacgggc cgccccaccg tcgtcggcta cacccgcgag gacatcgacg tctgggccga
     1381 ggtcgtggcc cgctccatcc gcgcctcggg cggccgcgcc ggccacaagg tgcacgtctc
     1441 ctacgggtac ggcctcttca ccggcggcct gggcgcccac tacggcgccg agagactggg
     1501 ctgcacggtc atccccgcct ccgggggcca gaccgccaag caggtccagc tcatccagga
     1561 cttcgagccc gacgtcatca tgatcacccc cagctacatg ctgacactgc tggacgagtt
     1621 cgagcatcag ggaatcgacc cgcgtagcac ctctctcaag gtcggtgtct tcggcgccga
     1681 gccctggacc gagaagatgc gcaccgagat cgaggagcgc ttcgacatcc acgccgtgga
     1741 catctacgga ctgtcggagg tcatgggccc gggcgtggcc aacgagtgcg tggagaccaa
     1801 ggacggcctg cacgtgtggg aggaccactt ctaccccgag gtgctcgacc ccctcaccca
     1861 ggacgtgctg cccgacggct cgaacgggga actggtcttc acctcgctga ccaagcaggc
     1921 catgcccgtc atccgctacc gcacccgcga cctgaccacg ctgcgcccgg gcaccgcccg
     1981 ccccatgcgg cggatggaca aggtcaccgg ccgcagcgac gacatgatca tcctgcgcgg
     2041 cgtcaacgtc ttccccaccc agatcgagga gatcgtcctg ggcctggacg ggctcgcccc
     2101 gcacttccag ctggtgctca ccaaggaggg ccgcctggat cgcatggccg tgcgcatcga
     2161 ggccgcaccg gactgcaccc ccgagcgccg tgtggccctg agcagggaga tcgtcgccac
     2221 ggtcaaggac aaggtgggcg tgagcgtgac cgtggacgtg gtcgaccccg aacagatcga
     2281 gcgctcggtg ggcaagttcc gccgcatcgt cgaccagcgc cccaaggagt agcgggaacg
     2341 ccgacagcgc gcggccgccg cggggcgggt gggaggaccc cgcggcggcc gtgccgtgta
     2401 cgcgggcggg tcaccagcgc atgagcgcga aaccgctggc catcccgccg ccgaaccccg
     2461 tcagcaggac ggtgtcaccg gggtgcacgg cccccgagcg gacggccgcg tccagggtga
     2521 cggggatgga ggcggctcct gtgttgccgt actcgcgaac ggtcgtgtgc acccgtgcca
     2581 cgggcagccc caggccccgc gcgagaccgt cgaccatgtg cccgttggcc tggtgcggga
     2641 ccaggtgggc gatgtcggcc tcccccaccc cggagtcctt caggaacgcg cggacggcgg
     2701 gcggaacgtg ctcgctgacg aagtcgcgca caccgcggcc gtccatcctg aagtagtgct
     2761 ctcccccggc gagggtcgcc tcgctggccg gtctgcggct cccgcccgcc ggcacccgga
     2821 tgaggtcgcg gtgggctccc gaactgctga gccgggtggc cagcagtccc cgcccccggg
     2881 gcaccgggcc gaggaccgcc gctcccgcgc cgtcgccgaa caggaccacg gtcctgcggt
     2941 cggccgggtc gagtatgcgg gagtacacgt ccgctccgat caccagggcg tagccgcccc
     3001 cggacgcgct gagtgcccgc tgcaccaccg acagcgcgaa gacgaacccg ctgcacaccg
     3061 cgttgaggtc gaacgccgcc gtgtccggtg tggcgccgac catctccgcg acggcggtcg
     3121 ccgtgggcgg ctggggcgag tccggggtgg aggtggcgac gacgacgaag gacaggtcgg
     3181 ccgggctgat cccggcccgc tccagcgcgg cgcgccccgc gtccgcggcc aggtcggacg
     3241 tcgcctctcc ggggtcggcc cacctgcggt ggcggatccc ggtcttgcgg acgatccacg
     3301 cgtcgtcgac gcccgcggga gcgcccacct cggtgttgcc gacctgtctc tcgggtaacc
     3361 gggagccggt gccgatcact ccgatcggca cggactcccg gaccttggtg tcttccatgt
     3421 gctctccgct tcgttgtggt cgatgctgct cggcgccgag ggggctcagg tgcggccggg
     3481 ccggacggcg gcccggccgc gccttcccgg agcggtcagg tcacgccgtc ggcgggtcgg
     3541 acggtgcggg gcatgttgat caggaccatc gcgccgatcg cgatgatgag cagggtgaag
     3601 tgcacctgct ggaccggggg cagcgcgatc cagtacccga accacagccc gcccacgagg
     3661 aacatcagtg ccagccccgc cagggagcag agcgcgatgt tgcccaggaa ggtcgcacgg
     3721 gttccgtctc cccgtccgag cagcaggcgc gtgtgcgtca cggtcgccgt ccacagcagc
     3781 agcacgctga gcgcctggta ggcgatcacc gcgtagagcg cgggcgcggc caggctcccg
     3841 gggagcgccc gcccgacgag cccgttgccg cggtccgggt cggcgatgat cccctccatc
     3901 gtcaggacct gcccgatgag ggtgatgttg gttccgctgt cggtgatgtt gttgaacatg
     3961 atcaggctca accaggcggc cataccgcca aggaccagcg tctgcgggta gagcagtccc
     4021 gtcgcgtcct cacgcgcgga ccggggtccg gtcctggacg ctcgccttgt ctgtaccatc
     4081 atcgaactcc tgtctccgtg ggccgcccgg caggctccga agcggtgtgg gtgtgggtgt
     4141 cggtccccgc cgaccagctc gcgagggcgc agccgcagca gcactcgtcc ccgtgccgac
     4201 cggtgcgtcg cgtggaccgt gcgccgccgc gggcgcgcgg tgacggcgag ggggcgtcga
     4261 gcacgggcca tccgcgcgcg atggcgtgtt cccgaagggc cggatcgcct tcgacgacat
     4321 gcgggtgccc gaccgcctcc agcagcgcga gatcactgat gtggtccccg tagccgtggc
     4381 tgtccggcag ggagagcccc cgggaggcgg cgaactccgc ggccgcctcc cccttggccc
     4441 gcccgatcat ggtgcgcccg acctcaccgg tgagcagacc gccgcggacc agcggttcgc
     4501 ttcccagggc cacggtcgct ccgagcgcct cggccaccgg gtccaggcag gcgaagaagg
     4561 agccggagag gaggacgacg tccgccccgg cggtgcggtg gcggtcgagt tcgcgtacgg
     4621 cgccggcgtg gaagaagtcc gggtccgcgg cccgctcggc gaaccagcgc cttccgctcg
     4681 cggcgatcct gtctcggggc tccccccggt agaagcggta gtaggcccgg ttgacggcct
     4741 cgcggggagc gccctttcgc gcccgtctgc gcagttccgc cgccatgcgc tcgtagaccg
     4801 agggcggctc gtcccgttca cgcaggtaga aggccaggaa gctgaacatg ctcttgcctg
     4861 agatcagggt ctcgtcgacg tcgaagaagg ccccgtaacg aggtccgtac cgccgttcgc
     4921 tctccgacac tgcgttcctc tctccggatg tcgcgggtgg ctgcggaccg gccgcaccgg
     4981 gcggccggtg ggtctggggc tcacaccagg gacgaggcgg catgttccgc ctcccgtacg
     5041 tccgtgtccg cgagcacgtg gtcgatggcc cgtccggcac ggcggtactc gaccgcctcc
     5101 agcacggcgg tgtcgaaggc ggtgaaggcg acctcacagc gcgccgagcg ccgaccgccc
     5161 tgctcgatgt ccacacgtgc ggtgaactcc aaccgcccgg ggtcggacag ctcggcggac
     5221 tggatgaggt agcggatgcg cgcgtcgacc gggaagagga agttctcgaa cgtggtggcc
     5281 agggagttga tcacgaagta gtagcggcgt tcgggccagc ggtgcgtgta gtaccgctcg
     5341 gtgacggcga ggaacatctg gcgcgccgcc tcgaccacga ccatgccctg ggtgtgctgg
     5401 cccgtctggt ggtcgagcag cagctcgttg tccccgtgca ggcgcagggc cgcgtggaag
     5461 acctggtcgt catggcgtcc gaggtcggcg atcagcacgt tctcctcgcg gtgcttgtgc
     5521 gcctccgcac gctcggcgag gacgggcgga cggtcgtgga ggacgacgcg ctcctccagg
     5581 ccgcggcgtc tgagcgcacc gcggatgtag tcccactcgt atccgccgat gccctgcccg
     5641 ccccgaagga cgagcgggcc ccggatgtcg tcgtagaccc ccgccctgag gccgcccacg
     5701 aacgaggaga gggcctggac ccgttcatgc accgcgaatc cagcgaaacg gtctcccacg
     5761 acacaaatcg atgtagcagt cttgtcagac attgcacaag cctttctggt atctgcgtat
     5821 atccgagggg tgaatattct tcgtacacga gaattcttgg tcgaagccaa gagcgttcgt
     5881 tcggagaaca gcggggagaa cgcagcggga aaccgtctgg ggcggggacg gtccgggagg
     5941 agggagaaaa gggggaggaa ggaaagggga aggaggtggg gcctgccggg accggccacg
     6001 tgcgctcgcg gggcggcgcc gaccgtgacc gggcccggcg ctcagggccg cacgcgggtc
     6061 gccgccccca gagcgagcag cgccccggct ccgaggattc ccgcggcggc cagcgacacc
     6121 gcggggaagc cgaacccggc gagggccgtg gccaactgcg gcccgagccc cgcaccgacg
     6181 aagaggaaga aggtgtagag cgcgacggcg gcaccgcgcg ccgcccccgc ctgcgcgccg
     6241 atggcctgga cgaggccggg tgccgccacc gcgatgccgg cgacgaagac gaagaggcac
     6301 acgccgatgg ccgcaccatc ggtgaggaac cccgtcacga ccgacagcag ggccgcgacg
     6361 ccgagcgcga tggcgacccg ccgctccggc gcggtccgcg ccagccacgg ggtgatcagc
     6421 gggatggcca gcatcgccgg gagtccgctg gcacgcagga tcagcagggc tccgtcgttg
     6481 cccgcgagcg acggaggacc cgcgacctgt aggccgctgt agacgccgac gaagcccagg
     6541 aggaccatca gcgtcgtccc gtagagcagc aggagcctcc tgttgcccaa aagcccggcc
     6601 atggccgcga aagcatcggc cacggtcgcc cggccgtcgg cctcgtccgc gcgcaggacc
     6661 aggcgcaacc cgaccgagcc cagggcgagt gcgacaccgc cggccaggaa gaccggctcc
     6721 cagcccgccg cctggccgat ggcctgggcc gcgagttgcc ccagaaccgc cgcggcgagg
     6781 aaggagctgg tcagccaggt gatggcggcg acacgccggt gcgggtcgat ccgggtggcg
     6841 atgtaggcga aggccgaggg agcgaaggcc gcggccacga gcccctggac cacacgcagg
     6901 acgagcgccg ccgccagcga tgacacgagg gggaacgcga tggtgaccac ggcggtggcc
     6961 gcgaggccca cgacgatcgt gcgacggggc ccgaaccggt cggcggccgg cccggcgaac
     7021 aggaagccga cggcgtaggc gatcgcgaag gcggtggccg tccaggcgac cgccgtgggg
     7081 ctactccccc agtcgccggc catgagggag aagagcggaa tgacggtgta gagctggccg
     7141 accaccagaa ttccggcgag tgagagcccc gcgatggtcg tcacaaatga caggcgtgaa
     7201 gaagccgggg cctctctcgc gacgccccgg tgttgtgtcg atgtcatgcc tccaatatct
     7261 agaccaacca gttggttgtc aacaggggaa tccttttacc ccaggccgga tccggccatg
     7321 tatcctgcct cacacattac aatatggaat catcccggga cccagtgcaa ccaactggtt
     7381 ggtaccgtat tatcctgacg tgatgacaaa gaggacggaa ataactcgcg cactcctgct
     7441 cagggccgca cggacggagt tcgccgcgta cgggatcgcg ggagcgcgcg tggaccgcat
     7501 cgccgagcgg gccggagtca acaaacagcg cctctacgcg aacttcggcg acaaggaaca
     7561 gctcttccag cacgtggtga gcgacgccct ggacgagctc tcccaggtcg tggccctcga
     7621 cgacggcacg gacccggccg cctacgtggc gcgcgtgttc gactaccaca ggacgaagcc
     7681 cgaactgctc aggctcctgc tgtgggagtc cctgcactac ggcgagaaac cgcttccgaa
     7741 cgaggaggac cggaccgccc tgtacgaggc gaagctctcc tccctcgccg agcggaccgg
     7801 cacgccgctc tcacgcgaca cgcggcacct gctgctgagc ctcatcggcc tggcggcgtg
     7861 gccgcagatc gttccccagc tcgcccggct gatcgtgggc ccggacatcc acgaggacga
     7921 ggcccaggca cgcctgcggg accacatcat cgccttcgtg cgaaacgcca cggaaacggc
     7981 tccgtccgtg cccgacaccg gcacggacgg agccctctga ccgtgtcggc gatcccgacc
     8041 ccgtgagagg gctggtcagg accgccggcc accctgctcg ctcacgctgc gccgtcggcg
     8101 agggcggcag gcggaggcgg ctgttcgccg ccgccctcca gcgcggcgat ccaggtgtcg
     8161 tgcagccaca tctgcgtggc gcgccgccgg gccgccgtgt cccaggtgac gtcccgctcg
     8221 aaggtcacca cccgtgagtc ggccccgacg atgacccacg cgtagcgtcc gtcctcgtga
     8281 cgggtcttga ccttgacacc gggaatgacg tgctcgcgcc cgtcctcgtc cacgaccgtc
     8341 tcgtcatggc ggtccaccca ctgctcggtc agaccccgga ggtagtcgtc gtcggtccgc
     8401 cccagccagc tgtaggcggc ctggcgcgcc tgctccgcgc ccggcaggtc ccgcgggtcg
     8461 ttgtgcgcgt ccgggtccag ggcggtgtat ccgacgacgc ggccgtcctc ccccctgacg
     8521 acggagacgt gcggtcctcc gagccggacc tcctccgtgg gctggtagcg cgtgaccgtc
     8581 accttctcgc cgtcgtggct cccggtgcgg acaccggcct cgtgccagcc ggagggaacg
     8641 gccagccccg cttcctccag ccgtgcggac cgcacaccgc ctccgccgtt ctcgtccgcc
     8701 gcggccaccg tctcgtccat accgtcctcc tcagtgatgt ccagcgcatg gtcggtcggg
     8761 cccggccccg gttcgctctc cccggcggcg taggcccaga tcgaggagcc gagcacggcg
     8821 gcgagcgcca tggcgaggac tccggccacc ggggaccgcg tcctcgcgga cccggccgta
     8881 cgcgttcgtg ttgccatggt cctcatccct tttcgcgacg tgtcgagagg gaccggagta
     8941 catctcgtgg ctcaggagct ccggccccga cacgagggca gcaccatgaa accaaccaac
     9001 tagttggatg tcaatggggc gcgacggcgg acggacaggg ccgggtcctc gcgtggtggc
     9061 cgggtgggcg ctggcggccg gctccggaac gcggccgcgg ccttgacgcc ggtcgcgtcg
     9121 acgccgacca tccgaatgcg gccctccccg cccgcaccct ggggccgcgc accgtcaggc
     9181 cgccccggcc acggcctccc accagcgcat aacctggctg acgtgcccac accagacatc
     9241 cgaccgggtc cggcggccgg gccgcgcgcc gccgggccgt cgcgggacgg cgcggtcgac
     9301 agccgcaggc tccccctcta cgccgccggt gagatcgacg tgccgttcgt cgtcaggggc
     9361 agtgccgaga tcatcccccg ggacacgttc tgggaggagc actcgcatcc gacgcacgag
     9421 ctgctctgga accagcgggg cgcgtcctcg gcgacgatcg gcgcgcgggt ctggacgatc
     9481 accccggcgg tcggcctgtg gctccccgca ggtacgcgcc actccggctg gacgcccgcg
     9541 ggaacgtggc accgcgccgc acagttcggc gtccacgcgg tgccgccgat ctccgacggg
     9601 cccgtggcga tggaggtcac gccgctcctg cgcctcctgc tcgaccggct cgacgccgac
     9661 gacctcaccc gggacgcgcg ttcgcggacg gaggcgatgg tgctcgacct cctcaccccc
     9721 gcccggcacg agctgatcct gcgcataccc gagtcgccgc tgctggcccc catcgccgcc
     9781 gccgtgcgcg aggaccccgc cgacgccacc accctggccg tgtgggcggc gcgcctcggc
     9841 gtgagcacac gcaccatcac acgggcgttc cgttccgaga ccggcctcgg gttcagccag
     9901 tggctggcca ccgcgcgtat gcagcacgcg gtcgcgatgc tcgcgcggga ggaggagatc
     9961 gacgaggtcg ccgcatgcgt gggctaccac tccgcgagcg cgttcggcgc ggccttccga
    10021 cggatcacgg ggttgagtcc gggacggttc cgctcggaga tccagtaggc gccgtgtcgg
    10081 gccccgggcc gacccaccga accaggtttg tccgtttcgc tacaaaaggt gtcacatcta
    10141 cgtgattgcg aactcaccaa ggacacccta aattcggtta ggcaaaccta atctctcggc
    10201 accgccgcgg ccgggtcgac gtgtgcgtcc cctccacgcc ctccgcccga ccgctcccct
    10261 ccggggcgca cgcccccgtg tgcgcccggg gcggtacggg agccggacca tggcaccgcc
    10321 atggctgagg ccacacggcc gcaatgggaa ggaaaccatg tcccgcgttt caaggctggc
    10381 cgcgctcggc acggccgcgc tgctcaccgc cgggctcgcc gcctgctcct cgtcctccgc
    10441 cgactcctcc ggcgactcct ccggcgggga cggcggcacc ggagactgga cccccgtcac
    10501 catcgagcac gccctcggca cgacgaccat cgagtccgag cccgagcggg tcgcgacggt
    10561 gaactgggcc aaccacgagg tcccgctggc cctgggcgtc gtgccggtcg gtatggccgc
    10621 cgccaacttc ggtgacgaca acggcgacgg cgtgctcccg tgggtcgagg agaagctcgg
    10681 cgaactggac gcggagacac cggtgctgtt cgacgagacg gacgggatcg ac
//
