LOCUS       NZ_MCOK01000001        11431 bp    DNA     linear   CON 22-OCT-2024
DEFINITION  Nocardiopsis sinuspersici strain UTMC 00102 NOSIN_1, whole genome
            shotgun sequence.
ACCESSION   NZ_MCOK01000001
VERSION     NZ_MCOK01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sinuspersici
  ORGANISM  Nocardiopsis sinuspersici
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 6071583)
  AUTHORS   Tokovenko,B. and Kalinowski,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2016) Helmholtz Institute for Pharmaceutical
            Research Saarland, Helmholtz Centre for Infection Research, Campus
            C2.3, Saarbrucken 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2016
            Assembly Method                   :: SPAdes v. 3.8.1
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina NextSeq; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001998325.1-RS_2024_10_22
            Annotation Date                   :: 10/22/2024 13:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,351
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 5,224
            CDSs (with protein)               :: 5,224
            Genes (RNA)                       :: 77
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 13 of 50
            Pseudo Genes (incomplete)         :: 44 of 50
            Pseudo Genes (internal stop)      :: 2 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:06:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1667290
            Orig. end                         :: 1678721
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCOK01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11431
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11431
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11431
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6431](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6431)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(538..2226)
                     /locus_tag="NOSIN_RS07015"
                     /old_locus_tag="NOSIN_06945"
     CDS             complement(538..2226)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627598.1"
                     /locus_tag="NOSIN_RS07015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_06945"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_077689959.1"
                     /transl_table=11
                     /translation="MNTLSAFRAFRALLPVLRPEWRGMAWIYLLGTMSALGLAGLTVLT
                     AWAVGHAVVERTPPGPLWWALVLGLVLLRVLLTWREMDVSHALAYRVLARLRMALFDAY
                     ARSVPGRRREHSGRAAAVAMDDIEKLEFFYAHTVAQLGASLTLFTASLVTASLLLPEAA
                     LVMLLGAAVVASSALYWARAARRLGEREQQERSDLSEHIVDALGALREVLAYGLAPRII
                     AETSAATARAATVAQRRELLSHLVTALRELVLTAVVIGVIATSATAAGVLSADVPVRLA
                     PAELPALVALALVGIAAIVDATDTLTRLHPLVSGAERVAAGINRPPVVAVPRAPRPLPA
                     GPLGLRFRDVSFSYEGRRPTLTSWSAGLAPGEHVGLVGPSGSGKSTVIALAARLWDPSA
                     GTVELVTADGGGVPVADLDEAALREAVALVDQDATLFHGTVRDNLLRGCGPRSDGDLNA
                     LLERVEAAAWIGLDDELGEGGVRLSGGQRARLCLARALARRPRVLLVDEVTASLDPATE
                     RLVSDVIAQFHGTVLIASHRAETLARLERVVDVAAAPHAVRRPGG"
     gene            complement(2223..3899)
                     /locus_tag="NOSIN_RS07020"
                     /old_locus_tag="NOSIN_06950"
     CDS             complement(2223..3899)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598922.1"
                     /locus_tag="NOSIN_RS07020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_06950"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_077689960.1"
                     /transl_table=11
                     /translation="MMIVPLLWREAARFPAALARSAALLVLVFLTHVGQAVAVAWSMSA
                     VLRGQAQDVLLALSLILGIALLRLALSLAQASAAAHLGGRVRQAVRRRAMEAALVPERL
                     HDTAARDGSMRASLGDGVDGIDAYVSKYVPAIVQLLLTCPIVVTALFSLSPWAGLCVAA
                     GVVFALLGPMAWKRTTARRGLDHWDSYEALSADLLESLRGMATLRTLGDVSGTRRRLDH
                     RSEALRRATERVMRVSLGETSVTDAAVQGGAVAAAAVAVVHAVTGQAPALEVYLILLLS
                     SEAFRPIRDLSRHWHAGFLGLTAVPGLQRLGAFTERRATAPDHDAPEHTASGHGDGRTA
                     RGRTADALRVTGLSFRYPGAHVDVIHDLDLTARRGSLTAIVGASGAGKSTLFDLLLGFL
                     TPRTGSIDLDGRPLRAFDIAVVSQRPVLFAGTVRDNLAVTGTTTEADLVEACRDAGVLN
                     EIQRFPRGFDTEVTEAGTSLSGGQRQRLALARALLARRPVLLVDEPTSALDADRSADVV
                     RTLHRVARDRVVLMISHRSETLTGVPDVFSLDSGHLYRSTS"
     gene            complement(3896..4819)
                     /locus_tag="NOSIN_RS07025"
                     /old_locus_tag="NOSIN_06955"
     CDS             complement(3896..4819)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NOSIN_RS07025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NOSIN_06955"
                     /product="hypothetical protein"
                     /protein_id="WP_077689961.1"
                     /transl_table=11
                     /translation="MAVPARERALLDGLWRGAGHHRLADGTVTRVRRRPVPSAGAAYPV
                     RTHLVVGADGPLDAGRYAFDLEDGTLHRRDEAREREAGWHVPGTGTDVTGTHLVLSVQP
                     GRSFGRYRHRAWPLWIADTAYALAAVEFLCAPAPSAVRLGPGPWLRDLLGVPRAAEHGR
                     WLARGLAPEIPLATVELPGSWSVVPGRGDALAARRSPATGEFEAAARAHDPRAVDVARA
                     SGQAWVLGAHRLETWSVAVDTPAAEFAQALWRAHRAAAGLCYAGALSGRWRCRPISGFT
                     ASRDRWTVHALAMLPGGHALDKESAA"
     gene            complement(5001..6431)
                     /locus_tag="NOSIN_RS07030"
                     /old_locus_tag="NOSIN_06960"
     CDS             complement(5001..6431)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598923.1"
                     /locus_tag="NOSIN_RS07030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_06960"
                     /product="YcaO-like family protein"
                     /protein_id="WP_227014449.1"
                     /sec_met_domain="YcaO (E-value: 4.6e-45, bitscore: 153.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPTTTGRPRPGTAGTATARADHPLPVDSLVDPECGVIRSVRAVT
                     HPAGAPTSYMGLTAAVADARQLGEWPADRVSLGTSFDDAGQARIAAIAEGVERYCGNWL
                     PADLPPDELRTGSRAELLAAGRRPVGLEDLPRFAPWQYARAGFPYQPLTEDTPALWARC
                     EERDGAEVWMPASLVYLNWRQSRFRHLPRVHHLNYAGIATGQGADDARDRGVLEIIERD
                     ALELWWHLDGPTFGIDPASVPGLADDLGGSSLDVFLTAMPSEFAPAVAALVHDRERGLY
                     AAGFSAALDPVRAARKAVLEAVHTWVYTQGCTDADGWVFRAVEQGLMARGLHLDFRADA
                     AYLDAAGAQCEHVIDLGAHVQIWLDPRVHGQARRFTAPALGLRPVTEVPAVSMDEVHRR
                     LADRGHRVLTRDLTTSDVRRTSLRVVRSFVTGLVPNAPAAFAYLGMPRFEHAALERGWR
                     TSWDGSPAGLTLVPPPHM"
     gene            complement(6428..7426)
                     /locus_tag="NOSIN_RS07035"
                     /old_locus_tag="NOSIN_06965"
     CDS             complement(6428..7426)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598924.1"
                     /locus_tag="NOSIN_RS07035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_06965"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_077689963.1"
                     /transl_table=11
                     /translation="MTEELGRAHLCRGRDGTVLRGRMWECGSPEGIVVIRTPYDADQHV
                     ATARSWNARGYHCLVQDVRGRYRSAGAWSPYTHEEEDGGQVLAHLLDEYGGLPLVLFGA
                     SYAAHTALEAARAVTRDGACPVAAIIALVPALGLAETAWDHEGSPQILHRIGWWHEHGR
                     HRRSQRPLSAAELDRRVAQARRQGVVEAAENWGWSPSASTGWRRLWSADRVDPASRYGH
                     VDVPLLVVSGDDDFFHDDARRLAREWRAPSHFASGPWGHRLVGGVADQALRARISAAGG
                     LTHIIDPWLAARGLPGSPAEWTGILTAASTPHTRSTLDPASEAWRHERTSP"
     gene            complement(7423..8313)
                     /locus_tag="NOSIN_RS07040"
                     /old_locus_tag="NOSIN_06970"
     CDS             complement(7423..8313)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558652.1"
                     /locus_tag="NOSIN_RS07040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_06970"
                     /product="hypothetical protein"
                     /protein_id="WP_077689964.1"
                     /transl_table=11
                     /translation="MLPQPSIARVPGTGAHVIVDSGGTGVVRTSSGRFLRLVSPPPGLL
                     DALAGASDGDGEASAYAEALRARMSALEEEDAQRRWPGPRREVALVGGGPLIDAVADAL
                     AGWGAAVTRHTDGGALLSTMDGAADGGRRGGGTAVPRGGGTRPWGLVVAYADSPVERVG
                     WDRLDTLPSQGVAWLRAYREGENCFVDPICLDGDDPGAEQVYRRRLAASFTPRELDTWR
                     RAAVAAAPASAAAHTLVVSRLLTLALAWAQESDALEGYRTTLWKLVTATGTVSEHPVLA
                     YDPPSSVDRGARRRV"
     misc_feature    8392..8405
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.63"
                     /tool="antismash"
     gene            complement(8397..8579)
                     /gene="amiA"
                     /locus_tag="NOSIN_RS07045"
                     /old_locus_tag="NOSIN_06975"
     CDS             complement(8397..8579)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017580478.1"
                     /locus_tag="NOSIN_RS07045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_06975"
                     /product="streptamidine family RiPP"
                     /protein_id="WP_077689965.1"
                     /transl_table=11
                     /translation="MDNQIVAEIEEPEQLAHLSASHSNALVENPFDVVAEIEEPEQLAH
                     LSASHSNALVENPFD"
     misc_feature    8582..8596
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.39"
                     /tool="antismash"
     misc_feature    8649..8665
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.28"
                     /tool="antismash"
     misc_feature    8654..8667
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.27"
                     /tool="antismash"
     misc_feature    complement(8654..8668)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.55"
                     /tool="antismash"
     misc_feature    complement(8655..8668)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.85"
                     /tool="antismash"
     misc_feature    complement(8656..8669)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.64"
                     /tool="antismash"
     misc_feature    8660..8675
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.2"
                     /tool="antismash"
     misc_feature    complement(8662..8675)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.12"
                     /tool="antismash"
     misc_feature    complement(8662..8676)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.91"
                     /tool="antismash"
     misc_feature    8663..8674
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.6"
                     /tool="antismash"
     misc_feature    8663..8676
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.96"
                     /tool="antismash"
     misc_feature    complement(8663..8678)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 23.03"
                     /tool="antismash"
     misc_feature    8664..8682
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.76"
                     /tool="antismash"
     misc_feature    8666..8681
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.2"
                     /tool="antismash"
     misc_feature    8668..8682
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.13"
                     /tool="antismash"
     gene            8696..9898
                     /locus_tag="NOSIN_RS07050"
                     /old_locus_tag="NOSIN_06980"
     CDS             8696..9898
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539017.1"
                     /locus_tag="NOSIN_RS07050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NOSIN_06980"
                     /product="hypothetical protein"
                     /protein_id="WP_077689966.1"
                     /transl_table=11
                     /translation="MNFQKLLVADYRGGRTYAVADGTVRPLGNAVLAEHAGFLTLPRRG
                     EGQAEWAFVDDRSGALVVSGARGERRFPAAIPGEHLACDASGRYVVVTTGLGANEKAWS
                     DVVTLVDLDRSESVRFRSRVGEPGAMIVPDQADGEPVVVLRHREPGALESFPLSRCLDA
                     ASHVPRITGEVVREIADDGHGDVVDQRTGIAATATGRGLERFVIDGGVPRMLGAVAWPV
                     PGRAYYLRLDPATGRAVGVVRGGPASPTAWTEWTNHLVDVDLSTGRTRCLGLPRGLAFR
                     FALGGTRAAVATIHPDGDELTVIERAEPRMRVPHRIPLPPMSRPPVPGRLPWDPVGGSP
                     AQRRSVAVDPAGTTVAVTRGGDGQLHLFHGGGLDTVTVPSPLDEGGHLYWHGGAADTIG
                     R"
ORIGIN
        1 cacgacgtac agcggcaggc ccctgtgccc ggcctcggcg gccgcccact ccagggcgcc
       61 gcgcgccgaa tccgacccgt cgacgcccac cgcgaccttg ggggagggcg ttcgttcctc
      121 agccatgtcc tcgcctctcg tccggcaagg gcgcgttccg cggatacgcg gaggccggga
      181 ggccccgtgc cccggggccg gacgtgctcc ggcggcgcgg cggacggcgc ccttgcctgt
      241 gcttcccccc tccgataccc ggctcacctg gagggcagtc gtgcggatgc tcccgagttc
      301 gtgcttccga ggcggcggca ccggatcccg ccccggcagg ggaggggccg caccgggcgg
      361 cgaaagccgt ccctgcccgg cccggctcgg gacggcggcc cggaccacgg cacttcggca
      421 tgtgcgggcg gctgcgcaag cggcggccga gggccgaccc gccgggcccg tgcgggtacc
      481 gccgtcccgg actccaacgg cgacggggcc cgaggtgggg acggccgctt cgcggagtca
      541 gccacctggt ctccgtacgg cgtgcggggc cgcggccacg tcgacgacgc gttcgaggcg
      601 tgcgagggtc tcggcccggt gggacgcgat gagaacggtg ccgtggaact gggcgatgac
      661 gtcggacacc agtcgttcgg tggccgggtc gaggctggcg gtgacctcgt ccacgaggag
      721 gacacgcggc cggcgtgcga gggcgcgggc caggcagagg cgtgctctct gcccgccgga
      781 cagccgcaca ccgccctctc cgagttcgtc gtcaagaccg atccaggcgg cggcttcgac
      841 gcgttcgagc agggcgttca ggtcgccgtc ggaccgtggt ccgcagccgc gcagcaggtt
      901 gtcgcgcacg gtgccgtgga agagtgtcgc gtcctggtcg accagggcga cggcctcccg
      961 cagtgccgcc tcgtccagat cggcgacggg gacgcccccg ccgtccgcgg tgacgagctc
     1021 gacggtgccc gccgaagggt cccagaggcg ggccgcgagg gcgatcacgg tgctcttgcc
     1081 cgaaccggag gggccgacca gcccgacgtg ctcgccgggg gcgagtccgg cggaccagga
     1141 cgtgagcgtt ggccgtcggc cttcgtagga gaaggacacg tcccggaagc ggaggccgag
     1201 gggtccggcg ggcagcggcc gcggagctcg ggggacggcg acgaccgggg ggcggttgat
     1261 tccggcggcg acgcgttcgg ctccggagac gagcggatgc agccgggtga gggtgtcggt
     1321 ggcgtccacg atcgcggcga tgccgaccag ggccagggcg accagcgcgg gcagttccgc
     1381 gggggccagc cgtaccggga cgtccgccga cagcacgccc gcggcggtgg cgctggtggc
     1441 gatgacgccg atgaccacgg cggtgaggac gagttcccgc agggcggtga ccaggtgcga
     1501 cagcagttcg cgccgttgcg cgaccgtggc ggcgcgtgcg gtggcggcgc tggtctcggc
     1561 gatgatgcga ggggcgagtc cgtaggcgag tacctcgcgc agggcaccca gcgcgtccac
     1621 gatgtgctcg gacaggtccg accgctcctg ctgttcccgc tcgcccagcc gccgggcggc
     1681 ccgcgcccag tacagtgcgc tgctcgccac gacggcggcg cccaggagca tgacgagcgc
     1741 ggcctcgggc agcagcaggg acgcggtcac gagtgaggcg gtgaacaggg tgagcgaggc
     1801 gccgagctgg gcgaccgtgt gggcgtagaa gaactcgagc ttttcgatgt cgtccatcgc
     1861 gaccgccgcc gcgcgtccgg agtgctcgcg gcgccgaccg ggaacgctgc gcgcgtaggc
     1921 gtcgaacagc gccatccgca gccgcgccag cacgcggtag gccagggcat gggagacgtc
     1981 catctcccgc caggtcagga gcacgcgcag cagcacgagt ccgaggacca gggcccacca
     2041 gaggggcccg ggcggcgtgc gctccacgac ggcgtggccg accgcccatg ccgtgaggac
     2101 ggtcaggccg gccaggccga gcgcgctcat ggtgccgagc aggtagatcc aggccatccc
     2161 ccgccattcg gggcgcagga cgggcagcag tgcgcgaaag gcccggaacg cggagagggt
     2221 gttcacgagg tgctcctgta caggtgtccg gaatcgaggc tgaacacgtc gggaaccccg
     2281 gtgagggtct cggagcggtg actgatcatg aggacgacgc ggtcgcgggc gacacggtgc
     2341 agagttcgga cgacgtccgc ggagcggtcg gcgtccaacg cgctcgtcgg ctcgtccacc
     2401 agcaggacgg ggcgccgtgc cagcagtgcg cgggccaggg cgaggcgttg gcgctgtccg
     2461 cccgacaggc tggtgccggc ttcggtgacc tcggtgtcga agccgcgcgg gaagcgctgg
     2521 atctcgttga ggactcccgc gtcgcggcag gcctcgacga ggtccgcctc cgtggtggtc
     2581 ccggtgaccg cgaggttgtc gcggacggtc ccggcgaaga gcaccggccg ctgggagacc
     2641 acggcgatgt cgaaggcgcg gagcggccgc ccgtcgaggt cgatgcttcc ggtgcgcggg
     2701 gtgaggaagc cgaggagtag gtcgaagagg gtggactttc cggccccgga ggccccgacg
     2761 atcgcggtga gggagccccg tcgggccgtc aggtcgaggt cgtggatgac gtccacgtgc
     2821 gcgccggggt agcggaaact cagaccggtc acccgcagcg cgtccgccgt cctgccccgg
     2881 gccgtccggc cgtccccgtg gccggacgcg gtgtgttctg gggcgtcgtg gtccggggcg
     2941 gtggcgcgcc gctcggtgaa ggccccgagc cgctggaggc cggggaccgc ggtgaggccg
     3001 aggaatccgg cgtgccagtg ccgcgagagg tcccggatgg gacggaacgc ctcggaggag
     3061 agcaggagga tcaggtagac ctcgagggcg ggggcctgcc cggtgaccgc gtggacgacg
     3121 gcgacggcgg cggcggcgac cgctcccccc tggacggccg cgtcggtgac ggacgtctcg
     3181 cccagggaga cgcgcatcac gcgctcggtg gccctgcgca gcgcctccga gcggtggtcc
     3241 agccgcctgc gggtgccgga cacgtcgccg agtgtgcgca gggtggccat gccgcgcagg
     3301 gattcgagca ggtcggcgct gagcgcctcg tagctgtccc agtggtcgag tccgcgccgg
     3361 gccgtcgtcc gcttccacgc catgggcccc aacagcgcga acacgacccc ggcggcgacg
     3421 cacaggcccg cccacggtga gagcgagaac agggcggtca cgacgatcgg gcaggtgagg
     3481 agtagttgca cgatcgcggg aacgtacttg gagacgtagg cgtcgatgcc gtcgacaccg
     3541 tcaccgaggc tggcacgcat cgaaccgtct cgggccgccg tgtcgtgcag ccgctcgggc
     3601 acgagcgcgg cctccatcgc gcggcggcgg accgcctgcc tgacccggcc gcccagatgc
     3661 gcggcggccg acgcctgggc cagggagagc gccaggcgca gcagcgcgat cccgaggatc
     3721 agcgacagcg cgaggagtac gtcctgggcc tggccgcgca gcaccgccga catcgaccag
     3781 gcgaccgcca cggcctgccc gacgtgggtg aggaagacga ggaccagcag ggcggcgctc
     3841 cgtgccagag ccgcgggaaa ccgggccgcc tcccgccaca gcaggggaac gatcatcatg
     3901 cggctgactc cttgtcgagg gcgtgtccgc cggggagcat cgccagggcg tggacggtcc
     3961 agcggtcgcg cgaagcggtg aatcccgata tcggacggca gcgccatcgc ccggacagcg
     4021 cgcccgcgta gcacaggccg gccgcggccc gatgggcccg ccacagggcc tgggcgaact
     4081 cggcggcggg cgtgtccacc gcgacggacc acgtctccaa ccggtgggcc ccgaggaccc
     4141 aggcctgccc ggaggccctc gcgacgtcaa cggcccgcgg gtcgtgggcg cgcgccgcgg
     4201 cctcgaactc gccggtcgcc ggggagcgcc tcgcggccag ggcgtccccg cgcccgggta
     4261 cgaccgacca ggagccgggc agttcgacgg tggccagcgg gatctccggc gcgagcccgc
     4321 gcgcgagcca ccggccgtgc tcggccgcgc gcgggacgcc gagcaggtcg cgcagccacg
     4381 gtccggggcc cagccgtacc gcggaggggg ccggagcgca caggaactcc accgcggcca
     4441 gggcgtaggc ggtgtccgcg atccacagcg gccaggcccg gtgacggtac cggccgaagc
     4501 tgcgaccggg ttgcacgctc aggaccaggt gggtccccgt cacgtcggtg cccgtacccg
     4561 ggacgtgcca gcccgcctcc cgttcgcggg cctcgtcgcg tcggtggagg gtgccgtcct
     4621 ccaggtcgaa ggcgtaccgc cccgcgtcca gggggccgtc cgcaccgacg accaggtggg
     4681 tgcgcacggg gtaggccgcg cccgcggagg gcaccggacg cctgcggaca cgggtcaccg
     4741 tcccgtcggc caggcggtgg tgccccgcgc cgcgccacag gccgtcgagc agggcgcgtt
     4801 cgcgggccgg taccgccacc gggtcggctc cggcacggtg ccagggactg gcgcgcggtc
     4861 cgggcggaag gggcggtccc ggtgcctcgg ccgagaaggc gcgtgcgagt gcgggcggca
     4921 ggcgcgaggg ctccgccgca ccgcgcctcg caccgtaagg agatggggtc gatgggaggg
     4981 gaaggggcat gggtgcggcc tcacatgtgc gggggcggga cgagggtgag gcccgcgggg
     5041 ctcccgtccc aggaggtgcg ccaccctcgt tcgagggccg cgtgctcgaa gcggggcata
     5101 ccgagatagg cgaaagccgc gggggcgttc gggacgagcc ccgtgacgaa gctccgcacc
     5161 acccgcagcg aggtgcgacg gacatcgctc gtcgtcaggt cgcgggtgag gacgcggtgt
     5221 ccgcggtcgg ccagccgccg gtggacctcg tccatcgaca cggccggcac ctcggtgacc
     5281 ggccggagcc ccagagccgg tgccgtgaag cgccgggcct ggccgtggac ccgggggtcg
     5341 agccagatct ggacgtgcgc gcccaggtcg atgacgtgct cgcactgggc gccggcggcg
     5401 tcgaggtagg cggcgtcggc gcggaagtcg aggtggaggc cgcgggccat caacccctgt
     5461 tcgacggcac ggaagaccca cccgtcggcg tcggtgcagc cctgggtgta gacccatgtg
     5521 tggacggctt cgaggacggc cttgcgggcg gccctcacgg gatcgagcgc cgcggagaac
     5581 cccgcggcgt acaggccgcg ttccctgtcg tgcacgagtg cggccacggc gggggcgaac
     5641 tccgagggca tggccgtcag gaacacgtcc agggagctgc cgcccaggtc gtcggccagt
     5701 ccgggaacgc tggcggggtc gatcccgaag gtgggtccgt ccaggtgcca ccacagttcg
     5761 agggcgtcgc gttcgatgat ctccaggact cctcggtccc tcgcgtcgtc ggccccctgc
     5821 ccggtggcga ttccggcgta gttcaggtgg tgcacgcgcg gcaggtggcg aaagcgcgac
     5881 tgccgccagt tgaggtagac cagcgaggcc ggcatccaga cctcggcgcc gtcgcgctcc
     5941 tcgcagcgcg cccagagggc gggggtgtcc tcggtgaggg gctggtaggg gaacccggcc
     6001 cgggcgtact gccacggagc gaaccggggc aggtcctcca ggccgaccgg gcggcggccc
     6061 gcggccagca gttccgcgcg ggacccggtg cgcagttcgt cgggaggaag gtcggcgggc
     6121 agccagttcc cgcagtagcg ctcgaccccc tcggcgatcg cggcgatcct ggcctgcccg
     6181 gcgtcgtcga aggacgtgcc caacgagacg cggtcggccg gccactcgcc gagctgccgg
     6241 gcatcggcga cggccgcggt cagccccatg taggaggtgg gagcgccggc ggggtgcgtc
     6301 acggcccgca cggagcggat gactccgcat tcggggtcga ccagcgagtc cacgggcagg
     6361 gggtggtcgg cccgggccgt agcggtcccg gcggtccccg ggcggggccg cccggtcgtg
     6421 gtgggggtca aggtgaggtc ctttcgtggc gccatgcctc tgacgcggga tcgagggtcg
     6481 accgggtgtg gggcgtggac gcggccgtga ggatcccggt ccactccgcc ggggagccgg
     6541 gcagtccccg ggccgccagc cacgggtcga tgatgtgggt cagtccgccc gcggccgaga
     6601 tgcgggcccg cagggcctgg tcggcgacgc cgccgaccag ccggtgcccc caggggccgc
     6661 tggcgaagtg gctcggggct ctccactcgc gtgcgagccg ccgggcgtcg tcgtggaaga
     6721 agtcgtcatc gccgctgacc accagtagcg gcacgtcgac gtggccgtag cgtgatgccg
     6781 ggtccacgcg gtccgcgctc cacagcctcc gccagccggt cgacgccgat ggcgaccagc
     6841 cccagttctc ggcggcctcg accacgccct gccttcgcgc ctgcgcgacg cgtcggtcga
     6901 gttcggccgc ggacagcggg cgttgggagc gccggtggcg tccgtgttcg tgccaccatc
     6961 cgatccggtg caggatctgc ggggagccct cgtggtccca cgcggtctcg gccagcccga
     7021 gggcggggac gagggcgatg atcgccgcga ccgggcacgc gccgtcgcgc gtcacggccc
     7081 gggccgcctc gagtgcggtg tgagcggcgt acgaggcgcc gaagaggacc agcggcagcc
     7141 ctccgtactc gtcgagcagg tgggcgagca cctggccgcc gtcctcctcc tcgtgcgtgt
     7201 agggggacca ggcccccgct gacctgtacc gtccgcgcac gtcctggacg aggcagtggt
     7261 agccgcgggc gttccacgag cgtgccgtcg ccacgtgctg gtcggcgtcg tagggggtgc
     7321 ggatcaccac gatgccttcc gggctcccgc attcccacat ccgcccgcgc aggaccgtgc
     7381 cgtcgcggcc ccggcacagg tgtgcgcgtc cgagctcctc cgtcataccc gccgacgcgc
     7441 ccctcggtcg acggaggacg gcgggtcgta ggccagtacc gggtgctcgc tgaccgtgcc
     7501 ggtggcggtg accagtttcc acagcgtggt gcggtagccc tccaacgcgt cagactcctg
     7561 tgcccaggcg agggccaggg tgaggagccg gctcaccacc agcgtgtgtg cggcggccga
     7621 ggcgggcgcg gcggccacgg ccgcgcgccg ccaggtgtcg agttcccggg gagtgaagct
     7681 cgccgccagg cgtcgccggt acacctgctc ggctccgggg tcgtccccgt cgaggcagat
     7741 cggatcgacg aagcagttct cgccctcgcg gtaggcgcgc agccaggcca cgccctggct
     7801 cggcagcgtg tcgagccggt cccagccgac gcgctccacg ggggagtccg cgtacgcgac
     7861 caccagtccc catggccggg tgccccctcc tcgggggacg gccgtgccgc cgccccggcg
     7921 accgccgtcg gccgctccgt ccatcgtgga gagcagggcc ccgccgtcgg tgtggcgggt
     7981 gacggccgcg ccccaaccgg ccagggcgtc ggccaccgcg tcgatgagcg ggcccccgcc
     8041 gaccagggcg acctcgcgcc gaggacccgg ccaacggcgc tgcgcgtcct cctcctcgag
     8101 cgcggacatc ctcgcccgaa gcgcctcggc gtacgcggag gcctccccgt caccgtccga
     8161 agcgccggcc agggcgtcca ggagaccggg cggtggggac acgagacgga ggaagcgccc
     8221 tgaggaggtg cgtacgacac cggtgcctcc gctgtcgacg atgacgtgcg cgccggtccc
     8281 cggcacacgg gcgatggatg gttgtgggag caaggcgacc tctcaaaggg cccggtcggg
     8341 cagcgcgggg ctgcccgacc gggtggttct cactgactac gtcccggagg ggagaactag
     8401 tcgaaggggt tctcgacgag cgcgttggag tgcgaggccg acaggtgggc gagctgctcg
     8461 ggctcctcga tctcggccac gacgtcgaag gggttctcga cgagcgcgtt ggagtgcgag
     8521 gccgacaggt gcgcgagctg ctcgggctcc tcgatctcgg cgacgatctg gttgtccatg
     8581 agggatttcc tttccaagcc atgacgtatc ggtcatctgt gccgatacgt gttcgtcggg
     8641 gcgaacgacg gtcactttac ttgaaaatga ttaccgtttc caatagcttt gccccatgaa
     8701 cttccagaag ctcctcgtcg ccgactaccg cgggggtcgc acgtacgccg tcgcggacgg
     8761 cacggtgagg ccgctcggca acgccgtcct cgccgagcac gccgggttcc tgacgctgcc
     8821 ccgcaggggg gaggggcagg ccgaatgggc cttcgtcgac gaccgctccg gagcgctcgt
     8881 ggtctcgggg gcccgcgggg aacggcgctt ccccgcggcc atccccggcg agcacctcgc
     8941 ctgcgacgcc tcgggccgct acgtggtggt gacgaccggg ctgggcgcga acgagaaggc
     9001 gtggagcgac gtcgtcaccc ttgtcgacct cgatcggagc gagtcggtac ggttccgctc
     9061 ccgggtcggg gagcccgggg cgatgatcgt ccccgaccag gccgacggcg aaccggtggt
     9121 cgtgctgcgc cacagggagc ccggagccct cgagtcgttt cccctctccc ggtgcctcga
     9181 cgccgcctcg cacgtgcccc ggatcaccgg tgaggtcgtc cgcgagatcg ccgacgacgg
     9241 acacggcgac gttgtcgacc agcgaaccgg catcgcggcg accgcgaccg gcaggggact
     9301 ggagcgcttc gtcatcgacg gcggtgtccc gcgcatgctc ggcgccgtcg cctggcccgt
     9361 gcccggacgc gcctactacc tgcgcctgga cccggccacg ggccgtgcgg tgggcgtggt
     9421 ccgaggcggg cccgcctccc cgacggcgtg gaccgagtgg acgaaccacc tggtcgacgt
     9481 cgacctgtcc accggccgga cgcgctgcct gggactgccc cggggactgg ccttccgctt
     9541 cgcgctcggc ggcaccaggg ccgccgtcgc cacgatccat cccgacgggg acgagctcac
     9601 cgtcatcgag cgggccgagc cgcggatgcg cgtcccgcac aggatcccgc tccccccgat
     9661 gtcgcgcccg cccgtccccg gacgcctgcc ctgggacccg gtcggcggct cacccgcaca
     9721 gcggcgctcc gtggccgtcg accccgctgg gacgacggtc gcggtgacca ggggaggcga
     9781 cggtcaactg cacctgttcc atggcggcgg actcgacacc gtcaccgtgc ccagcccgct
     9841 cgacgagggc gggcacctgt actggcacgg cggcgccgcc gacaccatcg gccggtgagc
     9901 gatgcacgac ttccctcctc ccctcgtccc cgccatcgac accgcgctca cgaagtggga
     9961 acggcacgga cgcccgggcc tgccggagaa cgggttcgcg ctcgcccgcc cgcccggacg
    10021 acgacgccag gggatctacc gggcggacac cggctcaccg gtgatcgagc ccgaggggca
    10081 catcctgcgc atggccccct ccccggacgg gcgcagcatc tgcgtccaac tggctgaacg
    10141 cgcggacgag gacgccgcgc tggggctggt cgacaccgcc accggatctc tgcggctcta
    10201 tccgcggatc cgctgccgtt acgaccccat gctctgggaa gacgactcgt ccggtgtcga
    10261 actggtcgcc ggccggtccc acagcctcgt ccgcctcgac gcgcgaaccg ggcggtcgac
    10321 ggcttcggcc gtaccgccgg gcacccggct cagactcttc cccggaggcc ggcgcggact
    10381 gctcgccgag agccgccccg gcagccccgg ccgcctcatc gaccgggcca ccggcgccga
    10441 gctcgccgag ttcgccgcca tcgtccgcgt cctgcccgcg ggtgaggacg tcgtcgtcga
    10501 cgacggcgcc cgccttcagg gcctcgatcc gggcagcggt gccccgaggt ggcgctggga
    10561 cgacccggac ctgcgggtca cctcgttcgc cgcccgcggc caggacatcg tcgccgtcgg
    10621 cgtacgcgcc ggccgcggtg tcatcgtgcg gctcgtccgg ggctcggtcg cggccgaacg
    10681 ggaggtcacc caccggggag ctccggctgt cgcgaccgga gtgagcgccg acgccgaccg
    10741 cttccacgtc ctcatcgagg gcccggccct gcccccacgg gtcgtacccg ccgaggagct
    10801 gctgtccgac cgtccggcgg cggcccgccc ggaagccccc acacgcaccg aacggtacgc
    10861 cttcaccgcc gaggacggat cgcgcgtacc cgtcgtggtc acctcgccga ccggcgccaa
    10921 gggccccgca ccgctgatcc tcacctgcta cggcggcttc ggtgtaccga gtctgccggt
    10981 gttcgaaccg accgtgccgg cgtgggccga gcacggtggc cggtacgcca tcgcacaggt
    11041 ccggggagga ggcgagcacg gctccgcctg gaggcaggcc gggcgcggcc tcgccaaggg
    11101 ccgcggcatc gaggacctgg ccgtcgccgc acgcggcctg gtcggggccg ggctcacccg
    11161 gcccgacctg ctcgtcctgg ccggcgcctc ccacggcgga gtcctggcca ccagttgcgc
    11221 cctcggatcg ccggacctgt gcgccggtgt cgtcagcacc gcggcgccgc tcgacctgct
    11281 caacctcggc gcccacccgc tcgggcggcg ctgggccgac gagttcgggg cctcgggggg
    11341 gatcgagcac ctgcgccgga tctcaccgct gcaccgtgct cgggaactca cccccggcac
    11401 cgctcctccc cgcttcctcg gcatcgtgct g
//
