LOCUS       NZ_CP014263            11944 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Spirosoma montaniterrae strain DY10 chromosome, complete genome.
ACCESSION   NZ_CP014263
VERSION     NZ_CP014263.1
KEYWORDS    RefSeq.
SOURCE      Spirosoma montaniterrae
  ORGANISM  Spirosoma montaniterrae
            Bacteria; Bacteroidota; Cytophagia; Cytophagales; Cytophagaceae;
            Spirosoma.
REFERENCE   1  (bases 1 to 5797678)
  AUTHORS   Kim,M.K., Srinivasan,S. and Lee,J.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2016) Department of Bio & Environmental
            Technology, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul
            139-774, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PacBio SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 72.42
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 01:24:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 4,981
            Genes (coding)                    :: 4,945
            CDSs (with protein)               :: 4,945
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 26 of 36
            Pseudo Genes (incomplete)         :: 12 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            Pseudo Genes (multiple problems)  :: 4 of 36
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:11:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2247922
            Orig. end                         :: 2259866
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014263.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11944
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11944
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11944
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6944](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6944)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(849..3491)
                     /locus_tag="AWR27_RS09800"
                     /old_locus_tag="AWR27_09710"
     CDS             complement(849..3491)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /locus_tag="AWR27_RS09800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWR27_09710"
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_077131010.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.3e-17, bitscore:
                     60.5, seeds: 73, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTDQLVKNGLVLAPYYACRQVGLPTDAIWKLGNFQIARLCDELT
                     HKEAELKWAASQLVDALYELIKYTTESDQSNQLLTLKRAIYNSRRVDEQHVKKSLPIHL
                     HTLFDNWSGRLNQLNETKQVAETAIGQSLAALNQAFGLLFGESSVQTAICHAQPELYRK
                     LLELPNAQNLNSQQRKVLLSGTAYAYRMATKTSPFSYFTRTGCWPLNQDERTSPDQPSS
                     VEEVNVLNIRIGQQLIDALLNQNLYSNKLPLLWNPASVLTEDGCYVLRTDLSLANNGAT
                     STESTVAMSANPILFLVKDIFSERITPIDQRELVALLIESLACDEEKAQRILTKLLEYK
                     LIIPTLGVDQNRLDWPAALAESPCVAHVSSALSNQLAGLALHVDSLAKTTPANRLNALQ
                     TLAQNSERVLASVGVVTPVGGIQKIVHNSVYLAAPPTPEPDQFIEQFPVFDSLLRVLPL
                     FNNDYALQQWVAGRLTDTDTPLPALIFFHRLFESAIDADPNTLNENHPLLNPQALVTGN
                     AMQQQITVQDRFVSEIASLLSETSEQLTLDSFFWAKWEEEASPFNTQSLPLSMTVMGQF
                     TNTWTTDAPRFVVNKLFAGYGTMFTEPPLSARSYRSNTMIERQLMDIEPACEYADIIAT
                     MGFQGQIRLPVGRRSLYYPGQQVSDPARQCFCWDNLFVKRDAAGNPVLIEGSTGKRIVP
                     LHRGTLASIYFPPFYRFLTTLGPSFTPDFSLYERLLNQAETQRVEGIRHYARIVSGGVV
                     LMRRAWSVPLAEVPRQSAKQSDFAYYRQVRAWASEVSMPKEVFVIPMHSATYYSEDGRG
                     LAFKRVHKPVYLNWDDYTSFRLFLRYTVFEAGRMSFIEALPILDRDKLGADNRVVEYQI
                     EMYQNDRLP"
     gene            complement(3494..5050)
                     /locus_tag="AWR27_RS09805"
                     /old_locus_tag="AWR27_09715"
     CDS             complement(3494..5050)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AWR27_RS09805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AWR27_09715"
                     /product="hypothetical protein"
                     /protein_id="WP_077131011.1"
                     /transl_table=11
                     /translation="MKEKILIITSPCSSFVNHVDRAVVRLTQLLKQCQIEYTVTLGGLA
                     FKQALGEYAHTDMVAFNTCEAEHLTLLDSLMTKSENNSAIWFVQDETELDLSFSEQYIT
                     HLSAAFPSIELVVFTNGLSSKMYRAWSLINVVRVIPKAILHALLTIDIQRITANATSSF
                     TTALYDCLNTYSSGELPADNTTDCFDLDATVDYSLLLKQHRDQTSFSQIVRLPVSLNEL
                     VSEQLTNHILGYSQQYKILDFDLGFVDLDNPETRVKLNEIATVFTSLSYSFRLHCTLPA
                     DSPAEYFAYLQRAKVDSVHLLPVVGAPDRPANDEETILLNLRQISLVKRLHGLGIEVQW
                     RLLLSRINLGSDLWVKFINELPVFFGFPAPIGVYWMQPKPPVSNAASDTADDAAILIAD
                     VLNGLRTCLGDWRTHPKDRYLSKGTGPAFARLFDRRGAIENWRFLNLNSAQTEFYQQLD
                     DVTQAAQIKERMAHIPADKIDNFLSFLEHHRLIVSINNGQYFLNASQRRDFTQAWATAE
                     L"
     gene            complement(5001..6944)
                     /locus_tag="AWR27_RS09810"
                     /old_locus_tag="AWR27_09720"
     CDS             complement(5001..6944)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="AWR27_RS09810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWR27_09720"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_077131012.1"
                     /sec_met_domain="TIGR03975 (E-value: 4.8e-156, bitscore:
                     518.8, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKVLIALMPWASIDFPGLGPTLVHSILNQQGYPCEMMYGNLAY
                     CHFLQGDRFVIEQVSKLPISEIAFSPLYFAVSKQTAADKLERHVEGTSQKGDEKRKSSA
                     YYLNIVEQAENCINYLFESTPWGDYDVVGFSVMMQQTVASLALAKKIKRHYPTIAILFG
                     GPSTSAPMGEAMIRAFPEIDYILQGEVDATIGPFVQELRQEKKDLRQIHGLLYRNNDGT
                     ISRTAPNRPFVKLDTLPVPDYGPYFSQLSQYGITNIEPYLQIETSRGCWWGEKHHCTFC
                     GIEDEILKYRTKSDDNVLNEIITLSSKHRGLEFFPVDSIISHGAFNSLLPKLTQLRSEF
                     GYDFSFFFECKSNLSTHHATRFRDAGVRSVQPGIESFSDNILRMMDKGTTGAKQVQCLK
                     LCAEREIVVNWNLIYENIGEREEDYEIMIRLIPFITHLPPLHAEGMIPVQLNRYAPLHN
                     TPERFGIVNIRPKGYYYDIFPDESIDFDNLAFYFDFDRNDKITPKLKALHSQLHDRLEA
                     WRENYQERTLVQRRGPGFIEIIDQRQLAYTGKDRLTVGRIVLEGIEAEMFSACDEVIRE
                     DSLVRLFIDRVDEGEIRQFVDRMVACYQIYRSESDQLINLPLRLDIPKRPTGADANEGK
                     NSDYNIALLELR"
     gene            complement(7133..7342)
                     /locus_tag="AWR27_RS09815"
                     /old_locus_tag="AWR27_09725"
     CDS             complement(7133..7342)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AWR27_RS09815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AWR27_09725"
                     /product="hypothetical protein"
                     /protein_id="WP_077131013.1"
                     /transl_table=11
                     /translation="MAEGSEKLQQTIDNVHSIVKKHVESGAIDDLDSMSVKELKLKVFG
                     ETERVLSNDEKASIISVVVYIAVT"
     misc_feature    7563..7576
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.09"
                     /tool="antismash"
     misc_feature    8485..8500
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 21.15"
                     /tool="antismash"
     gene            9047..>9160
                     /locus_tag="AWR27_RS25750"
                     /pseudo=""
     CDS             9047..>9160
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281585.1"
                     /locus_tag="AWR27_RS25750"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNLRRDGFEAIADPTVSKHLQIFADCELLRQEQNGREL"
     gene            9333..9557
                     /locus_tag="AWR27_RS09830"
                     /old_locus_tag="AWR27_09735"
     CDS             9333..9557
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /locus_tag="AWR27_RS09830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWR27_09735"
                     /product="DoxX family protein"
                     /protein_id="WP_335695418.1"
                     /transl_table=11
                     /translation="MLAQGLAQLFQTKGYVDILAHLGYPLYLLYIIGVWKLLGVVAIVR
                     PSDGPAALAGIARSDWGIVVFQTRRQKNH"
     gene            9580..10137
                     /locus_tag="AWR27_RS09835"
                     /old_locus_tag="AWR27_09740"
     CDS             9580..10137
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027744.1"
                     /locus_tag="AWR27_RS09835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWR27_09740"
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_198045123.1"
                     /transl_table=11
                     /translation="MKKDLSPDQRAEFINTLQVRFEKNRSRHDGFDWTTMQAKLEANKN
                     KLWSLHEMERTGGEPDVVGYDDATGEYIFYDCSAESPKGRRSVCYDQQALDSRKEHKPQ
                     TSAAEMAAEMGIELLTEEQYRALQTLGTFDAKTSSWVKTPADIRQLGGALFADHRYGHV
                     FVYHNGAESYYGSRGFRGLLRV"
     gene            complement(10098..10505)
                     /locus_tag="AWR27_RS09840"
                     /old_locus_tag="AWR27_09745"
     CDS             complement(10098..10505)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AWR27_RS09840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AWR27_09745"
                     /product="hypothetical protein"
                     /protein_id="WP_077131016.1"
                     /transl_table=11
                     /translation="MGRYLFAGSLLVFGGLHFLFAPFIATLIPAWIPWPLFWAYFVSVA
                     FVATAISLFLNRDVSISGVWLGSMFLLWVMMLHAPRAVAKPHIEPEWTSLLIALAMSGV
                     AFVIAGLSHRADRPLSKQNQTRSNPRNPLEP"
     misc_feature    complement(10831..10846)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.36"
                     /tool="antismash"
ORIGIN
        1 ctgccgttga tcgagctgtt gtaaactcac ccaccgtttc cgcagttcca cttgccattg
       61 gtgcaagaaa gcttgtacgg cggggctttg gccgggcaca tgcatcagag ccgacatgtc
      121 tgagcctgac aaccagtatt gtagttgagt tagaacgggt tcaaggtcgg caatttgctc
      181 gtcaaatgtg gggaaaccac gactaacata ggtatcttcc accgaatcat caggatgatt
      241 tttaagcagc agtccggttt ggctggcccg atggaacaga tcggcaatcg cgcctggctg
      301 atcatcgata acaccaacca tcatcatcga cagatgcata agcagggtaa tacgggcggg
      361 cagattatcc cgcgtcaact ttaacaaccg tccggcaaac actgacgaaa acgcgaaatg
      421 atcttcgctc aatgcctgtg cttcgtcagt tccatagcgg tgtttctcaa accgatacgg
      481 atgcagttcg atacccggct ccgggcgtag cggctcaata ttttcaacct caacagcgcc
      541 aagtttatcg tacaacgccc gcatgttgtt ggcatacgca ttgtaatcgt ggaccggctg
      601 agctacgtgc ggatggtatc gtagatgttc tgagaaggaa ttggcaagct gggcaagggc
      661 atcggcttcg tcggtacagg cgaacagccg cagccgcaga tgatcgcccc cattccagta
      721 ccgcagaaac gcgtattgac tgatacgtcc gtttttctgc cagttaacca gtattggcaa
      781 gacataatcc agcagcagcg catcctgatg gccacgataa tagatatgga tcgacgtgac
      841 ggcacgattc atggtaatcg gtcgttttgg tacatctcta tctgatattc aaccacccga
      901 ttgtctgcac ctaacttatc gcggtcgagt atcggtagtg cttcgataaa gctcattcgg
      961 ccagcctcga ataccgtata tcgtaaaaac agccgaaagc tggtgtaatc gtcccagttc
     1021 agataaacgg gtttgtgtac acgtttgaag gccaggccac gcccatcttc tgaataatac
     1081 gtagcagaat gcatgggtat aacaaacact tctttgggca tactcacctc tgatgcccat
     1141 gctcttacct gtcgataata cgcaaaatca gattgtttgg ccgattgtct gggcacctca
     1201 gcaagcggca ccgaccaggc acgacgcatc aacacaaccc caccgctcac tatgcgggca
     1261 taatggcgta tgccttctac gcgctgtgtt tcggcctggt tcagcaaccg ttcgtacagc
     1321 gaaaaatcgg gcgtaaacga tggcccaagc gtggtcagga accggtagaa gggcggaaaa
     1381 tagattgatg ctaacgtacc ccggtgcagc ggtacgattc gcttgccggt tgacccttcg
     1441 ataagcacag ggttaccagc cgcatcgcgt ttaacaaaca agttgtccca gcaaaagcac
     1501 tgccgggccg gatcactcac ctgctggccg ggatagtata aacttcgtct gcctaccggt
     1561 agtctgatct gcccctgaaa ccccattgtc gctataatgt cggcatactc acaggctggc
     1621 tctatatcca ttaactgtcg ctcaatcatc gtgtttgacc ggtagctccg ggctgacaat
     1681 ggcggttcgg taaacattgt accatacccc gcaaatagtt tattgacaac gaaacgaggg
     1741 gcgtcagttg tccatgtatt cgtaaactga cccattacgg tcatcgacag cggaagcgac
     1801 tgcgtattga aaggggatgc ttcctcctcc cacttcgccc agaaaaagga gtcgagcgta
     1861 agttgctccg acgtttcgga tagcagcgaa gcaatctcgg atacaaagcg atcctgaacg
     1921 gtgatttgtt gctgcatggc gttgcccgtg accagagcct gcggatttag cagggggtgg
     1981 ttctcgttca gcgtatttgg gtctgcgtcg atagcagact cgaaaagtcg atgaaaaaaa
     2041 ataagcgcgg gcaacggcgt atcggtatcg gtcaacctac cagccaccca ctgttgaagc
     2101 gcgtagtcgt tattgaacag cggaagaaca cgtagtaggg aatcgaaaac ggggaactgc
     2161 tcaatgaatt ggtctggttc cggagttggc ggagcggcca gatacacact gttatgaaca
     2221 attttctgga ttcctccaac cggcgttacc acaccaaccg aagccagtac gcgctcactg
     2281 ttctgagcga gtgtctgtag tgcgtttagc cgattggcag gtgttgtttt ggccagcgag
     2341 tctacgtgca atgccaaccc ggcaagctga ttgcttaacg cactcgatac gtgcgctacg
     2401 caggggcttt cggcaagagc agccggccag tcgagccgat tctgatcaac acccagtgtc
     2461 ggaataatga gtttatattc cagcagtttg gtcagtattc gctgtgcttt ttcttcatcg
     2521 caggctaatg attctatcaa taaggccacc agttcgcgct ggtcgatggg cgtgatgcgc
     2581 tcagaaaaaa tatcttttac cagaaataaa atggggttag cggacatagc gaccgtcgat
     2641 tcggtcgacg ttgctccatt gtttgccaac gacaaatcgg tacgtagcac gtaacagccg
     2701 tcttccgtca gtaccgatgc cggattccag agcaggggta gtttattgct atataagttc
     2761 tggttcagca gcgcgtcgat tagttgctgt ccaatacgaa tattcagtac gtttacttct
     2821 tccaccgatg acggctgatc gggggatgtt ctctcgtcct gattgagtgg ccagcagcct
     2881 gttctggtaa aataactgaa ggggctggtt ttagtagcca ttcggtaagc ataagccgta
     2941 cccgacagca atactttgcg ttgttggctg ttcagatttt gggcgttagg cagttcaaga
     3001 agtttacgat ataactcggg ttgggcatgg catatggccg tttgaacaga tgactcgcca
     3061 aataataaac caaacgcctg attcagcgca gcaagcgact gaccaatagc cgtttcagcc
     3121 acttgcttcg tttcgttcaa ctgattcagc cgaccgctcc agttgtcgaa tagtgtatga
     3181 agatgtatcg gcaaactttt tttgacatgt tgttcatcaa cccgtcggct attgtaaata
     3241 gcccgcttta gggtaagtag ttgattactt tggtctgatt ccgttgtgta ttttatcagc
     3301 tcataaagcg cgtcaaccag ttgagaagct gcccatttga gttctgcttc tttatgcgtc
     3361 aactcatcgc ataaccgggc tatctgaaaa ttacctaatt tccagatagc gtcggttgga
     3421 agccctacct gccgacatgc atagtaagga gccagcacaa ggccgttttt gacaagttga
     3481 tcggtacgca tatttataat tcggcggtag cccaagcctg cgtaaagtct ctccgttgag
     3541 aagcattaag aaaatactga ccattattga tcgatacaat caagcgatga tgctccagaa
     3601 acgacaaaaa attatctatc ttatctgctg gaatatgcgc catccgttcc ttaatctgtg
     3661 cggcctgggt aacatcatct aactgctggt aaaactctgt ctgagccgaa ttcaaattca
     3721 gaaaacgcca gttttctatg gcaccacgcc ggtcgaacag tcgggcaaac gcggggccgg
     3781 tacccttcga caggtagcga tccttggggt gcgttcgcca gtcgcccaaa caagtacgca
     3841 acccgttcag tacgtcagcg ataagtatcg ctgcgtcgtc ggcagtatca gacgctgcat
     3901 ttgataccgg tggcttcggc tgcatccaat acactccgat tggagcggga aagccgaaaa
     3961 aaacgggtaa ttcattaata aacttcaccc acaagtcgct cccaaggttg atccgagaca
     4021 acaacagccg ccactgtacc tctatcccca atccatgcaa tcgcttaacc agactgattt
     4081 ggcgcagatt caacagtatg gtttcctcgt cattggctgg cctatccggt gctcctacaa
     4141 ctggcagcag atgcaccgag tcaactttgg cccgttgtag gtacgcaaaa tactcagccg
     4201 ggctgtcggc aggcagggtg caatgtaatc taaagctgta tgacaaggat gtaaagacgg
     4261 tggctatttc attcagttta acgcgggttt cgggattgtc taaatccaca aatcctagat
     4321 caaaatcgag tattttgtat tgttgcgaat accctaaaat atggtttgtg agttgttctg
     4381 atactaattc gtttaaactg acgggtaatc ggacaatttg gctaaagctt gtctggtctc
     4441 tgtgttgttt cagcagaaga ctataatcaa cggtcgcatc gaggtcgaag caatccgtcg
     4501 tattatcagc gggcaattcg cctgaactgt aagtattcag gcaatcatac agcgcagtgg
     4561 tgaaactact ggttgcattg gctgttatcc gctgaatatc aatggtcaat agtgcgtgca
     4621 ggattgcttt gggtataaca cgaaccacat tgatgagcga ccaggcccga tacatttttg
     4681 acgacagccc gttggtaaat accactaatt caatgctcgg aaaagcggca gagagatgcg
     4741 taatgtattg ctcagaaaag ctaaggtcca gctcggtctc gtcctgcaca aaccagatgg
     4801 cactgttgtt ttcagactta gtcatcaggg aatcaagtag agtcagatgc tccgcttcac
     4861 acgtgttgaa cgcgaccatg tcagtgtgtg catactcgcc cagagcctgt ttgaaagcta
     4921 acccgcccag tgtgacggtg tactcaatct gacattgttt caataactgt gtcaggcgga
     4981 caacagcccg atcaacatga ttaacgaagc tcgagcaggg cgatgttata atcagaattt
     5041 tttccttcat tagcgtctgc acctgtcggg cgtttaggaa tgtcaagacg aagcgggaga
     5101 ttaatcaact gatcactctc tgaccggtat atctggtagc aggcaaccat ccggtccaca
     5161 aattgccgaa tctcgccttc atcaactcgg tcgatgaata gcctgaccag gctatcttcc
     5221 cgaatcactt catcgcaggc cgaaaacatc tccgcttcga taccttcaag cacaatacga
     5281 cctacagtga gacggtcttt acccgtataa gcaagttgcc gctggtcgat aatttcgata
     5341 aagccggggc ctctgcgctg aacaagagtg cgttcctgat aattttcccg ccacgcttcg
     5401 agccggtcgt gaagctgact gtgcaaagct ttgagtttgg gagttatttt atcgtttctg
     5461 tcgaaatcga agtagaaagc cagattatcg aagtcaatgg attcgtccgg aaaaatatca
     5521 tagtaatatc ccttaggtct aatatttact ataccaaaac gttcgggcgt gttgtgtaaa
     5581 ggcgcgtagc gattgagctg taccggaatc attccttcgg catgtagggg cggcaggtgc
     5641 gtgatgaatg gaatcagccg aatcattatt tcataatcct cttcgcgctc acctatattt
     5701 tcatagatca gattccagtt tacgacaatc tctcgttcag cacacagttt caggcattgc
     5761 acctgcttgg ctcctgttgt gcccttatcc atcatacgta gtatattatc gctgaagctt
     5821 tctatacccg gttgaaccga ccgtactcct gcgtctctga atcgagtagc atggtgcgta
     5881 gaaaggttgg atttacactc gaagaagaaa ctgaaatcat agccaaattc gctacgtagc
     5941 tgagtaagct tgggtagtag tgagttaaac gccccgtgac tgatgatgct atctaccgga
     6001 aaaaactcaa gtccccgatg cttactcgat aacgtaataa tctcgttcag tacgttatca
     6061 tccgatttag ttcgatattt cagaatctcg tcttcaatac cgcagaacgt acagtggtgt
     6121 ttttctcccc accagcaccc cctcgacgtc tcaatctgta aatagggttc gatattggta
     6181 atgccatact ggctaagctg gctaaaataa ggcccgtaat caggtactgg aagcgtatcc
     6241 aatttaacga aaggtcggtt cggcgcggtt ctggaaatcg ttccgtcatt gttccgatac
     6301 agtaagccgt gaatttgtct caagtctttc ttctcctgcc ggagctcctg cacgaaaggc
     6361 ccaatagtag catcgacttc accttgtaaa atataatcta tctcgggaaa agcacggatc
     6421 atggcttcgc ccattggagc gcttgtactg gggcccccaa acagtatggc aatagtgggg
     6481 tagtgtcttt taattttctt agctaaggcc agcgaagcaa cggtctgttg catcatcacg
     6541 gaaaaaccaa ccacatcgta atctccccaa ggcgtactct cgaataaata attgatacag
     6601 ttttctgcct gttcgacaat attgaggtaa tatgcgctcg atttacgttt ttcatctcct
     6661 ttttgactgg ttccttctac gtgacgctca agtttgtcag cagccgtttg cttactcacc
     6721 gcgaaataaa gcggagagaa tgctatctct gaaataggca gttttgaaac ctgctcaata
     6781 acaaagcgat ccccctgtag gaaatgacaa taagccaaat ttccatacat catctcgcac
     6841 ggataaccct gctggtttag aattgaatgg acaagggttg gccccagccc cggaaaatca
     6901 atactggccc atggcataag ggctattaac actttcttgt ccattattta tctattttag
     6961 acactaaagt tattcgacaa caaattcatt cacatagttg attcttttaa ctttactcaa
     7021 ccattgttat ttatcaataa agcgttctgt acctaaatta gtgaagaagc tgtataaaac
     7081 aggaaaacag tttctctgtt ttatacagcc atcgacgcat tggtcataat acctacgtta
     7141 cggctatata cacaacgacc gaaataatgc tggctttttc gtcgttgctc aatacccgtt
     7201 cggtctcgcc gaagactttt agcttcagct ctttaaccga catgctgtcc aaatcatcga
     7261 tagccccgct ttcgacatgc tttttaacga ttgaatggac attgtcaatc gtttgttgca
     7321 gtttttcact accctcggcc atagttttaa aggtttaaag gatgaatact tctgtaccat
     7381 cagtggatta tagatccctt aggtgtgtga aatctaatat ttataaattc aatgctttta
     7441 aaaatatact ggaaggtata tgttgtgcta atagcatgct aaaaaataac caagaactaa
     7501 gtttggtaaa tatcagcttt gaacttgcaa cagcatagtg gcgattactt cgatacgtat
     7561 acttttgtaa caaaaacgtt tcgagtcctg gctcaaactg atccacgcgg gtttctggtc
     7621 aattacgcca acggggccat ctttgacgag gttcagcggg tgccggatct gtttgcttat
     7681 ttataaggta tcgtagatcg gcagacggct caacagccct ttgtgttgac aggctcccaa
     7741 aacttcctcc tgctggaaag tatcaaccaa agtccgcaga ccgaatcgcc gttttacgct
     7801 tgctgcccct cagctatctc gtcacttagc acccgccgac ttctgtccca gctacatcca
     7861 aacctatctc gaccgggata tagggtcact gaaaaacgtt gccgacctca gtacgttcac
     7921 ccgcttccta acgaattacg tggtctatct gcttccgccc tattatgaga acttcagcaa
     7981 acgactcatc aaatcgcctg aactgtactt ctatgacaca ggactccttt gttcgctact
     8041 gggcttatgg tcgagtccgt gtcctgacgg cacaacgtaa cggcagagcc aggaatcgtg
     8101 agggttaata cgacgctttt gagtgtgatt tgctgaaata agtgcctgat tttgtttgtc
     8161 aaaagttgat aacccacgtt aatttgacaa attcaaggca tacagggtcg ctcaacggtg
     8221 ttggttttgt tgcgcaaatt tggtagcttg gactggcagt tcccccctcg tcccagtatg
     8281 aaagctaaac cgcctgtggc caggccacct acccctgagc aacgcatcaa agagttagaa
     8341 gccgctctac aacgagaaaa ggaccgggtt ctggcttaca agacgcttat ttgaggtagc
     8401 cgagaaggag cacggcctca ccatccgaaa aaaggcttcc cccgcagagt ccatcggctc
     8461 cggcaagagc ggcaaatgag tctggcctac atttgtcaac tgttcgggat gagccgccag
     8521 agctattacc aggcccagca gcgttgccag caggtgaaga ctcaagtgat tgcgttagtg
     8581 cagcaacaac ggcggctcat gcccagggtg ggtacgcgca agctatacta tttattgaaa
     8641 gagccgtttc agcaacaacg aatcaaagta ggccgggaca gtttatttag ctgcttacgg
     8701 gatgaagatt tgttggtgcg accggtacgc agttatcaca aaacaacaga ttccgggcat
     8761 tggatgcgtg acccgtcctg aaataagctg gtgtttagag cgtgttttgt tgatggtaga
     8821 caaggtggtc acgaactacc catgtgccgg gtagtaatcc aagtttgagc gacgaacata
     8881 gccgatagcc ccggatacac aaccgatttg gcccgttaaa tcgcctgaat aagccacgac
     8941 gcagcattgt tgtgacgagc ttcatcaacg aaccaatgac caaaatttcg gaaatgcttg
     9001 cgtagtcaaa tagctacgcg tatatttgta gccaaatgac tacgcgatga atttaagacg
     9061 agacggtttc gaggccatcg ccgacccaac agtctcaaag cacctgcaaa tctttgccga
     9121 ttgcgaattg ctccgacagg agcagaatgg ccgggagttg tgtacagatt ggtcgcggcc
     9181 agagaccaaa tgctgcgatt acccttcgca caagggctga acatagccca caaccgatta
     9241 cagacccttg taggccaggt aaaagctaac gcgctatgac aaaaagagac aaaatcattt
     9301 attgagttgc cacgggcttc ctggcgtttg gtatgctggc acaagggctg gcgcagttat
     9361 ttcagacaaa agggtacgtg gacattctgg ctcacctggg ttaccccctg tacttgttgt
     9421 acatcattgg cgtctggaaa ctgcttggcg tagtagcgat tgtgcgaccc agtgacggcc
     9481 ctgctgccct cgctggtatt gctcggtctg attggggtat cgtggtattt cagacccgcc
     9541 gacaaaagaa tcattaggac ttaccaataa atcaacaaca tgaaaaagga cttatcacca
     9601 gatcaacggg ccgaattcat taacacgttg caagtccgtt ttgagaaaaa cagaagccgc
     9661 catgacggtt ttgactggac taccatgcag gcaaagctgg aagcgaacaa gaacaagctg
     9721 tggtcactcc acgaaatgga acgaacgggc ggtgaaccgg atgttgttgg ttatgatgac
     9781 gcgacgggcg aatacatttt ttatgactgc tcggcagaga gtcctaaagg tcgtcgaagt
     9841 gtttgttacg accagcaagc tttggattca agaaaagaac acaaacccca aaccagtgcg
     9901 gcagagatgg cggctgaaat gggcatcgag ttgttgacgg aagaacaata ccgggcgttg
     9961 cagacacttg gaacgtttga tgcgaaaaca tcgagttggg tgaaaacgcc cgccgacata
    10021 agacaactcg gtggtgccct ctttgctgac caccgctacg ggcatgtttt cgtgtatcac
    10081 aacggagcag aatcgtacta tggctccaga ggatttcgcg ggttgctacg ggtctgattt
    10141 tgcttagaca ggggccggtc tgcacgatgc gacaaaccgg ctatgacaaa cgcaacgcca
    10201 ctcatcgcta aggcaatcaa caggctcgtc cactccggct cgatgtgcgg cttagccacg
    10261 gcgcggggcg cgtgcagcat catgacccat agcaaaaaca tactgcccag ccaaacgccc
    10321 gaaatagaca cgtcccggtt caggaataaa ctaatggccg tggccacgaa cgccacacta
    10381 acgaaatacg cccagaacaa cggccacgga atccaggccg gaatcagggt ggcgatgaac
    10441 ggggcaaaca gaaagtgcag cccgccaaaa accagcagcg acccggcaaa cagataccgc
    10501 cccaccggct caagcgtctg cccggattac catctgtgcg caaaccgtta tcacccggcg
    10561 aaaccctacc ggccagtacc aatgcccctc cagttagcgc aacaaactcg aaaaaaggaa
    10621 gcgttgccct gaacaaacat acctgaaagt cagtcgtgaa cccaccaata aacatcgtcc
    10681 aacggccatc gatgaagact caaatattgt agaaacggag tagctaaaca aaggattaag
    10741 atagacggtg tgtttttact gaatcgcgtt ccccttcaca acttatccaa atccaactgc
    10801 tgaatcagag ctggattttc gggtgtgagc gtgaaataga cctcaatctt cccgcgttcc
    10861 ccttcgacta cgaacgtgcc acgtagctga ttttcaggca ctaactcccc tacgttcagt
    10921 actttcccaa tcgtcgcgaa cagggcctgt gcctgtttcc gacgcgtgtc tgctgacttg
    10981 tcggggaaga agttctcggc aaaaatgccg ctgcgctcgg cgttggtgaa gtcgggcagg
    11041 agtcgtacca gctcggcttt gcgctgggcc agaatgggcg aaacgggcag ttgacggggt
    11101 tgtagcctgc caatcgtcac cagcgtatcg agtacggcgg tgtttgcgct gcccagcccg
    11161 gcgtaggtca ggtttccgta ggcaattacg ccgatgccgt agtcgggcag aatgcgccac
    11221 tgactgccga agccgggcaa cccaccacta tgccccaccg ccacccggcc agtgcagttc
    11281 tgactccagc ctaatccata gccgtatcca ctcgtaacgg cacacacctg cccggcggca
    11341 tttttcgcct gcgcattcaa acccgaaaat acccagggtt gctgcatctc gcggatcgag
    11401 ctgcgcttta caggtgatgc ggcattccgg gcggtatcgg cgttgctgcg cgggggccag
    11461 gccgcctggt gcagcgccac gtatttactg aaatcttcga ttgatgtaat cagcccgccc
    11521 attgcgccgt agctgccgtc gcgtaaaagt ggttcttcga gccaggtgtc gttctgccag
    11581 cggtagccta aagccagccg gtcggcggga acgcggtcgt attcccactg cgtatcggtc
    11641 atgccgagcg gtttcagaat agtgtcggta atgtactgct ggtaaggttt gccagacacc
    11701 aacgtaataa tgcggcccag catcgcaaaa ccgaggttgc tgtattcata ggctacaccc
    11761 ggcgcgtttg agttggtgat tccacctttg atcaggttca gtaaatcagc atcggagtcg
    11821 gctaattggc ggtcgcccca gggattgtct tcggggaaac ccgccgagtg cgtcatcagg
    11881 ttgcgtaccg taatgcgggg cgaatcggtg gtcaggtatt tatgcttttt gagttcggga
    11941 atat
//
