LOCUS       NZ_LZNS01000001        10396 bp    DNA     linear   CON 15-JUL-2024
DEFINITION  Streptomyces sp. MP131-18 scaf_01, whole genome shotgun sequence.
ACCESSION   NZ_LZNS01000001
VERSION     NZ_LZNS01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. MP131-18
  ORGANISM  Streptomyces sp. MP131-18
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7861428)
  AUTHORS   Paulus,C., Rebets,Y., Tokovenko,B., Nadmid,S., Terekhova,L.P.,
            Myronovskyi,M., Zotchev,S.B., Rueckert,C., Kalinowski,J. and
            Luzhetskyy,A.
  TITLE     New natural products identified by combined genomics-metabolomics
            profiling of marine Streptomyces sp. MP131-18
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7861428)
  AUTHORS   Tokovenko,B. and Rueckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2016) Helmholtz Institute for Pharmaceutical
            Research Saarland, Helmholtz Centre for Infection Research, Campus
            C2.3, Saarbrucken 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 72x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001984575.1-RS_2024_07_15
            Annotation Date                   :: 07/15/2024 02:52:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,130
            CDSs (total)                      :: 7,069
            Genes (coding)                    :: 6,890
            CDSs (with protein)               :: 6,890
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 62 of 179
            Pseudo Genes (incomplete)         :: 155 of 179
            Pseudo Genes (internal stop)      :: 7 of 179
            Pseudo Genes (multiple problems)  :: 45 of 179
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:18:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6694659
            Orig. end                         :: 6705055
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LZNS01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="34"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5396)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(497..1786)
                     /locus_tag="STBA_RS29870"
                     /old_locus_tag="STBA_60680"
     CDS             complement(497..1786)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1143: sugar-binding lipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004991654.1"
                     /locus_tag="STBA_RS29870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_60680"
                     /product="extracellular solute-binding protein"
                     /protein_id="WP_077062161.1"
                     /transl_table=11
                     /translation="MHRHRVAALSCSAVLTAAALTACGGSDDSGSGPVDLTYWAWADME
                     QVVDMWNEANPDIQVTVKEQAAGDDLVTRTITAAQAGQGPDLVQAEYQALPTLVSNDVL
                     ADISGQVADVEDQFAPGLWQQVTLGTDAVYALPQDSGPMMFFYRHDLFEEYGLTVPTTW
                     DEFAETARTVRDVAPDTYLTTFSANDAGQFAGLAQQAGAQWWTTEGETWQVAIDDEATR
                     RVAEFWGGLVAEDAILNEPMYTPAWNQAINSGEQIAWVSAVWAPGSLTTAAPDTAGNWA
                     MAPLPQWEAGENATGSWGGSTTAVTRDSEHAEAAAEFATWLNTDPEALAGLVREGGIYP
                     AATAAQTGEALAEPPAFFPNQPDFYAQAAEIAAGTAPAAWGPNVNVAYSTFNDAFGEAA
                     QSRGDFPAALTAMQRSVVEDLESHGFQVSE"
     misc_feature    complement(1817..1831)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.41"
                     /tool="antismash"
     misc_feature    1818..1832
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.61"
                     /tool="antismash"
     misc_feature    complement(1826..1843)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.89"
                     /tool="antismash"
     misc_feature    1863..1880
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.03"
                     /tool="antismash"
     gene            1924..3924
                     /locus_tag="STBA_RS29875"
                     /old_locus_tag="STBA_60690"
     CDS             1924..3924
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174265.1"
                     /locus_tag="STBA_RS29875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_60690"
                     /product="beta-galactosidase"
                     /protein_id="WP_077062162.1"
                     /transl_table=11
                     /translation="MERLAFGGDYNPEQWPQEVWPQDVALMREAGVTLVSVGIFSWALL
                     EPREGHYEFGWLDRLVDLLHEGGIRVDLATPTVVPPAWFYRAHPEALPVSREGVRWEFG
                     SRGAICHSSPAYREAATRITRALGERYGSHPALELWHVHNEYGVPVLECYCDTSAAHFR
                     RWLAERYGTVEALNAAWGTAFWGQAYGEWEDVRPPRLTPTTANPAQRLDFARFASDSAL
                     ENFRAERDILHELSPGIPVTTNFMVSPTQCDTMDYWKWGREVDIVTNDHYLVAEDDRNH
                     INLSFAADLTRSVGAGRPWLLLEHSTSAVNWQPRGIAKRPGEMARNSLAHVARGSEGAM
                     FFQWRASRAGAEKFHSAMVPHAGTDSRIWREVVELGADIGALQRIKDTRTVADAAIAWD
                     WESWWAQKLEWRPSDDLDARERAESWYAALFDHHLTVDFVHPEAEGPTDHPLLVVPALY
                     LATRAAGENLRRYTERGGTLVVSCFSGIVDAHDAVHPGPHPGALRDVLGLVVEEFQPLR
                     AGESVRLAGPDGALLTADVWAETVRTTGAEPVWTYADGPAAGGPAVTRHRIGGGTAWYV
                     SARLGQDGLRAVLAAATADAGLAPREDLPRDVEVVRRSGAGGDFLFAINHTADEATVTP
                     PAPGTELLTGARVTGPLAVPAGAVRVVHLGT"
     gene            complement(4081..4980)
                     /gene="thpD"
                     /locus_tag="STBA_RS29880"
                     /old_locus_tag="STBA_60700"
     CDS             complement(4081..4980)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828636.1"
                     /locus_tag="STBA_RS29880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_60700"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_077062163.1"
                     /sec_met_domain="PhyH (E-value: 8.3e-39, bitscore: 132.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVATPRQDPVVWSEPGARGPFAAGELA
                     AFERDGFAAIDQLITPEEVLAYRAELDRLVTDPDIRADERSIVEPTSQEIRSVFEVHKI
                     SEKFAGLVRDPRVLDRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     RMRTVSVSIALTENFDTNGGLMIMPGSHLTFLGCAGETPKDNYKKSLQMQDAGTPSDEA
                     LTDYADRHGIKLFTGRAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENAAVEPFA
                     APVPRPDYIGARDFTPVR"
     gene            complement(5001..5396)
                     /locus_tag="STBA_RS29885"
                     /old_locus_tag="STBA_60710"
     CDS             complement(5001..5396)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="STBA_RS29885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_60710"
                     /product="ectoine synthase"
                     /protein_id="WP_077062164.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.7e-54, bitscore:
                     181.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSDIEGTDRDVQSASGTWRSKRIVLAKEGVGFSLHETVLY
                     AGTETTMWYANHIEAVFCVEGEAELTNENTGEKHWIKPGTMYLLDGHEKHTVRPKTDFR
                     VMCVFNPPVTGREDHDENGVYPLLTEV"
     gene            complement(5435..6703)
                     /gene="ectB"
                     /locus_tag="STBA_RS29890"
                     /old_locus_tag="STBA_60720"
     CDS             complement(5435..6703)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 6.2e-73.
                     Score: 237.7. Matches aSDomain:
                     nrpspksdomains_STBA_RS29890_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835756.1"
                     /locus_tag="STBA_RS29890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_60720"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_077062165.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.5e-74, bitscore:
                     248.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFTTLESEVRSYCRGWPTVFDRAQGSYLFDEDGHTY
                     LDFFSGAGSLNYGHNNPVLKRALLDYLERDGITHGLDMSTTAKRAFLESFQNVILRPRD
                     LDYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDDYLDGQTPDFIWFERLLEDTGSGLNKPAATIVETVQGEGGINVARP
                     EWLRGLADLCRRHDMLLIVDDIQMGCGRTGPFFSFEEAGIVPDIVTVSKSISGYGLPMA
                     LTLFKPELDIWEPGEHNGTFRGHNPAFVTAAAALDTYWTDGQMEKQTLSRGEQVEASLR
                     AIAMEHAHAGASYRGRGLVWGMTFDESARASRVCRRAFELGLLLETSGPESEVVKLLPA
                     LTTTPEELDEGLRVLARAIRETA"
     aSDomain        complement(5609..6619)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STBA_RS29890_Aminotran_3.1"
                     /evalue="6.20E-73"
                     /label="STBA_RS29890_Aminotran_3.1"
                     /locus_tag="STBA_RS29890"
                     /protein_end="365"
                     /protein_start="28"
                     /score="237.7"
                     /tool="antismash"
                     /translation="FDRAQGSYLFDEDGHTYLDFFSGAGSLNYGHNNPVLKRALLDYLE
                     RDGITHGLDMSTTAKRAFLESFQNVILRPRDLDYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDDYLDGQTPDFIWFER
                     LLEDTGSGLNKPAATIVETVQGEGGINVARPEWLRGLADLCRRHDMLLIVDDIQMGCGR
                     TGPFFSFEEAGIVPDIVTVSKSISGYGLPMALTLFKPELDIWEPGEHNGTFRGHNPAFV
                     TAAAALDTYWTDGQMEKQTLSRGEQVEASLRAIAMEHAHAGASYRGRGLVWGMTFD"
     gene            complement(6770..7306)
                     /gene="ectA"
                     /locus_tag="STBA_RS29895"
                     /old_locus_tag="STBA_60730"
     CDS             complement(6770..7306)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969939.1"
                     /locus_tag="STBA_RS29895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_60730"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_077062166.1"
                     /transl_table=11
                     /translation="MDSEFPAGREGLTLDTPRLDDGAALWRLARDSRVLDLNSSYSYLL
                     WCRDYARTSIVVRDAAGAPVGFITGYTRPQAPRTLLVWQVAVDGGQRGRGLAAAMLDAL
                     VLRTGRELGVNTIETTVTPDNTASNRLFASFAERHGAAVEREVLFDAGVFPERGHQPEV
                     LYRIGPIPEPAAAVR"
     misc_feature    complement(7508..7523)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7518..7533
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7518..7535)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.31"
                     /tool="antismash"
     misc_feature    complement(7529..7544)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     gene            complement(7698..8339)
                     /locus_tag="STBA_RS29900"
                     /old_locus_tag="STBA_60740"
     CDS             complement(7698..8339)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180702.1"
                     /locus_tag="STBA_RS29900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_60740"
                     /product="hypothetical protein"
                     /protein_id="WP_077062167.1"
                     /transl_table=11
                     /translation="MGDRPDARELLHAAAAAHRPDRARMLARVEHGTARAGPRRRAARL
                     RVTAAAVGFAVTLALATLAVGWAGSDDGRPTGPARPSAAERLASSGAVDPDSHAYWSQS
                     NVTVATREPLTSFAVEVRVVAEAGMRPTGGWRTLPGDDFDLSVANEDGDLVFRWKLRPG
                     AEVPPGEHVFAAQYDHDLGARGAGLDRYRVTGSGPSGPVAASGGFDTSAG"
     gene            complement(8368..8943)
                     /locus_tag="STBA_RS29905"
                     /old_locus_tag="STBA_60750"
     CDS             complement(8368..8943)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004603.1"
                     /locus_tag="STBA_RS29905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_60750"
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_077062168.1"
                     /transl_table=11
                     /translation="MSGPSDEFHAFFEQHHAELSRLARLLLGGTEGADGADDLAADALV
                     AIWHRWDRVRAADHPLAYARGVVANLARSRIRSRVRERRRIALFWSRGVEPAEGPDVPA
                     VLDVRAALGRLPYRKRACVVLRHAFDLSEQETARALGISVGTVKSQTSRGMAELQRLLV
                     EAAAPEGEGGTGEFLRALRTGPVQRRSW"
     gene            9063..10187
                     /locus_tag="STBA_RS29910"
                     /old_locus_tag="STBA_60760"
     CDS             9063..10187
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006132696.1"
                     /locus_tag="STBA_RS29910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_60760"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_077062169.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 5e-16, bitscore:
                     57.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSERPVLPPVLHIRGRVLVGPGPDGGPDLDVRDELWVVGGRITYE
                     RPRGARETVRLTGWVLPGLVDAHCHVGLDAHGAVDRAAAEKQALADRAAGALLLRDAGS
                     PADTRWIDARTDLPHLIRAGRHIARTRRYIRNYAQEVEPADLVAAVRHQAARGDGWVKL
                     VGDWIDRERGDLAPCWPAGALRAAIAAAHEAGARVTAHCFAEDSLPDLVAAGIDCVEHA
                     TGLTEETIPLFAAHGVAIVPTLVNIATFPGLADGGEAKYPRWSAHMRRLHARRYDTVRA
                     AHDAGLDVFTGTDAGGALPHGLIAREVAELRTAGIPVHDALGAATWRARDWLGKSGLTE
                     GAPADLVVYADDPRQDLGILADPQRVVLRGQIVA"
ORIGIN
        1 gcgtacagcg cgccgccgcc gagcaggtcg ggctccggca ggccgagcgc gtcgagggtg
       61 tggtgcagcg ggctgacgga cggcaggtag aggaagcccc acagcagggc ggcgatcacg
      121 cctggcacgg cgtagggcag gaagatcacc aggcggccga agcggcgtcc gcggacccgg
      181 tcggcgtcga ggatcagcgc gaacagcagc gcgagcccga gcatgacggg caccacgatg
      241 agcccgtaca cgccgacgcg cagggcgccg tcgagcagtt cgggatcgga cagcgcgtcg
      301 gcgtagttgg acagcccggc ccagacctcg ctgcgggcgc cgctgccgag gccgaggccc
      361 tgcacctcga tcgtgcgcag actcaggtgg atggcgtagc cgatgggcag ggcgaagaac
      421 agcaggaaca ggacgatggc cggggcgagg aaggcccacg gggcgcgccg ccgcgccccg
      481 ggccgccggc ggcgggtcac tcgctcacct ggaagccgtg ggattccagg tcctcgacga
      541 cggagcgctg catggcggtg agggcggcgg ggaagtcgcc gcggctctgt gcggcctcgc
      601 cgaaggcgtc gttgaaggtc gagtaggcga cgttgacgtt ggggccccag gcggcgggcg
      661 cggtgccggc ggctatctcg gcggcctgcg cgtagaagtc cggctggttg gggaagaagg
      721 cgggcggctc ggccagggcc tcgccggtct gcgcggcggt ggcggcgggg tagatgccgc
      781 cttcgcggac cagcccggcc agcgcctcgg ggtcggtgtt cagccaggtg gcgaactcgg
      841 cggcggcctc ggcgtgttcg gagtcgcgtg tgacggcggt ggtggagccg ccccagctgc
      901 cggtggcgtt ctcgcccgct tcccactgcg gcagcggcgc catggcccag ttccccgcgg
      961 tgtcgggggc cgcggtggtg agggagccgg gcgcccagac ggcgctgacc caggcgatct
     1021 gctcaccgga gttgatcgcc tggttccacg cgggcgtgta catgggctcg ttgaggatgg
     1081 cgtcctcggc gaccaggccg ccccagaact cggcgacgcg gcgggtggcc tcgtcgtcga
     1141 tggcgacctg ccaggtctcg ccctcggtgg tccaccactg ggcgccggcc tgctgggcga
     1201 gtccggcgaa ctggccggcg tcgttggcgg agaaggtggt gaggtaggtg tcgggggcga
     1261 cgtcgcggac ggtgcgcgcg gtctcggcga actcgtccca ggtggtgggg acggtcaggc
     1321 cgtactcctc gaagaggtcg tgccggtaga agaacatcat cggcccggag tcctgcggca
     1381 gggcgtagac cgcgtcggtg ccgagcgtga cctgctgcca caggccgggc gcgaactggt
     1441 cttccacgtc ggcgacctgt ccggagatgt cggcgagcac gtcgttgctg acgagggtgg
     1501 gcagcgcctg gtactcggcc tgcaccaggt cggggccctg gccggcctgg gcggcggtga
     1561 tcgtcctggt gacgaggtcg tcaccggcgg cctgctcctt gacggtcacc tggatatccg
     1621 ggttggcctc gttccacatg tcgacgacct gctccatgtc cgcccaggcc cagtaggtga
     1681 ggtcgacagg gccggagccg gagtcgtccg agccgccgca ggcggtcagc gcggcggcgg
     1741 tgaggacggc ggagcaggag agtgcggcga cgcggtgtcg gtgcatggtt cctccgggcg
     1801 gatgacgggc cggtgtctgg gagcgttcac agtagagaaa cagtgccgat acttgtcaat
     1861 ggttgttgct gtgcggttat gttgcctgtg cacgttcaca gaaaggcgcg gagaaaaatg
     1921 cacatggaac gactcgcgtt cggcggggac tacaacccgg agcagtggcc gcaggaggtc
     1981 tggccgcagg atgtcgcgct gatgcgtgag gccggggtga ccctcgtcag cgtcgggatc
     2041 ttctcctggg ccttgctcga accgcgcgag ggacactacg agttcggctg gctggaccgc
     2101 ctcgtcgacc tgctgcacga gggcggcatc cgcgtcgacc tggcgacccc caccgtggtg
     2161 cccccggcct ggttctaccg cgcgcacccc gaggcgctgc ccgtcagccg cgagggcgtg
     2221 cgctgggagt tcggctcacg cggcgcgatc tgccacagct cgcccgccta ccgcgaggcc
     2281 gccacccgga tcacccgcgc gctcggcgaa cgctacggca gccaccccgc actcgaactg
     2341 tggcacgtgc acaacgagta cggcgtcccg gtcctggagt gctactgcga cacctcggcc
     2401 gcgcacttcc ggcgctggct cgccgaacgg tacggcaccg tggaggcgct gaacgcggcc
     2461 tggggcaccg ccttctgggg ccaggcgtac ggcgagtggg aggacgtccg gccgccccgg
     2521 ctcacgccca ccaccgccaa ccccgcccag cgcctggact tcgcccgctt cgcctccgac
     2581 agcgccctgg agaacttccg cgccgagcgc gacatcctgc acgagctgtc ccccggcatc
     2641 cccgtgacca ccaacttcat ggtctcgccc acccagtgcg acaccatgga ctactggaag
     2701 tggggccgcg aggtcgacat cgtcaccaac gaccactacc tggtggccga ggacgaccgc
     2761 aaccacatca acctctcctt cgccgccgac ctcacccgct ccgtcggcgc gggccgcccc
     2821 tggctcctcc tcgaacactc caccagcgcc gtcaactggc agccgcgcgg catcgccaag
     2881 cgccccgggg aaatggcgcg caacagcctg gcgcacgtgg cccgcggctc cgagggcgcc
     2941 atgttcttcc agtggcgcgc ctccagggcc ggcgcggaga agttccactc ggcgatggtg
     3001 ccgcacgccg gcaccgacag ccgcatctgg cgcgaggtcg tcgaactcgg cgcggacatc
     3061 ggcgcgttgc agcggatcaa ggacacccgc accgtcgccg acgcggccat cgcctgggac
     3121 tgggaatcct ggtgggccca gaagctggag tggcgcccga gcgacgacct ggacgccagg
     3181 gaacgcgccg agtcctggta cgccgcgctg ttcgaccacc acctgaccgt ggacttcgtg
     3241 cacccggagg ccgaggggcc gacggaccac ccgctgctgg tcgtgcccgc cctctacctc
     3301 gccacccgcg ccgcgggcga gaacctgcgc cgctacaccg agcgcggcgg caccctcgtc
     3361 gtctcctgct tctccggcat cgtcgacgcg cacgacgccg tccacccggg cccgcacccg
     3421 ggcgcgctgc gcgacgtact cggcctggtc gtcgaggagt tccagccgct gcgcgcgggg
     3481 gagagcgtgc ggctcgccgg tcctgacggc gccctgctga ccgcggacgt gtgggccgag
     3541 acggtgcgga ccacgggcgc cgagccggtc tggacctacg cggacggccc ggcggccggc
     3601 ggccccgccg tcacccgtca ccgcatcggc ggcggcacgg cctggtacgt gtccgcgcgg
     3661 ctcggccagg atggactgcg tgccgtcctg gccgcggcca ccgccgacgc cggtctcgcc
     3721 ccgcgcgagg acctgccccg cgacgtcgaa gtggtccggc gctccggcgc gggcggcgac
     3781 tttctcttcg cgatcaacca caccgccgac gaggccacgg tgaccccgcc ggcccccggc
     3841 acggaactgc tcaccggcgc gcgcgtgacc ggccccctgg ccgttcccgc gggagccgtg
     3901 cgggtggtgc acctcggcac ctgaacggca tctgaacggc accgcccccg ctcgacgcgc
     3961 cacgggtcaa tcactgtcac tcatggaagg ggccgcggat caccggccgc ggaccgcggt
     4021 caccgcacga acgggcgccg gccaccctcg gggtggccgg cgcccctgcg cgtccgggcc
     4081 tcagcgcacc ggggtgaagt ccctggcgcc gatgtagtcc gggcggggga ccggcgccgc
     4141 gaacggctcg accgccgcgt tctccacgct gttgaacacg atgaagacgt tgctgcgcgg
     4201 gtacggggtg atgttgtcac ccgagccgtg catgcagttg cagtcgaacc aggtggcgga
     4261 ccccgcccgg ccggtgaaca gcttgatgcc gtgccggtcc gcgtagtcgg tcagcgcctc
     4321 gtcggacggg gtgcccgcgt cctgcatctg gagggacttc ttgtagttgt ccttcggcgt
     4381 ctcgcccgcg cagccgagga acgtcagatg cgagcccggc atgatcatca ggccgccgtt
     4441 ggtgtcgaag ttctccgtca gtgcgatcga gaccgacacc gtgcgcatgc gcggcagccc
     4501 gtcctcggcg tgccacgtct cgaagtccga gtgccagtag aagccgctgg cgccgaaccc
     4561 cggcttgacg ttgatccggc tctggtggac gtacacgtcc gagccgagga tctgccgggc
     4621 ccggtcgagc acccggggat cacgcacgag tccggcgaac ttctcgctga tcttgtgcac
     4681 ctcgaacacc gagcggatct cctgcgacgt gggctcgacg atcgagcgtt cgtcggcgcg
     4741 gatgtccgga tcggtgacca gccggtcaag ctccgcgcgg taggccagca cctcctccgg
     4801 cgtgatcagc tggtcgatgg cggcaaagcc gtcccgttcg aatgccgcga gttccccggc
     4861 ggcgaacggc ccccgggcgc cgggctcgga ccacaccacc gggtcctggc gcggcgtggc
     4921 cacctcggtg gccccgcggg tcgggtacag gtcggcggtg cgttcgggtg cggtggtcat
     4981 ggggtgcttc cttcctctcc ttacacctcg gtgagcagcg gatagacgcc gttctcgtcg
     5041 tggtcctccc ggccggtgac cggcgggttg aagacacaca tcacccggaa gtcggtcttg
     5101 ggacggaccg tatgcttctc gtgcccgtcc agcaggtaca tcgtcccggg cttgatccag
     5161 tgcttctcgc cggtgttctc gttggtgagc tctgcttcgc cctccacgca gaacacggcc
     5221 tcgatgtggt tcgcatacca catagtggtc tccgtacccg cgtagagcac ggtctcgtgc
     5281 agcgaaaaac cgacgccttc cttggccagc acgatgcgct tgctgcgcca cgtgcccgag
     5341 gcggactgca cgtcccggtc ggttccctcg atgtcgctga gcgatcggac gatcacggcg
     5401 ttgtacttcc cttccggttt tcggttgtgg gctttcaggc ggtttcgcgg atggcgcggg
     5461 cgaggacgcg cagtccctcg tccagctcct ccggggtggt cgtcagcgcg gggagcagct
     5521 tgacgacctc gctctcgggg cccgaggtct ccagcagcag ccccagctcg aaggcgcggc
     5581 ggcacacgcg ggaggcgcgg gcgctctcgt cgaacgtcat gccccacacc aggccgcggc
     5641 cgcggtagga ggccccggcg tgcgcgtgct ccatggcgat ggcgcgcagc gacgcctcga
     5701 cctgctcgcc gcggctgagg gtctgcttct ccatctggcc gtcggtccag tacgtgtcga
     5761 gcgccgcggc ggccgtcacg aacgcggggt tgtggccgcg gaacgtgccg ttgtgctcgc
     5821 ccggctccca gatgtcgagt tccggcttga agagggtcag cgccatcggc agcccgtagc
     5881 cgctgatcga cttcgacacc gtgacgatgt cgggaacgat cccggcctcc tcgaaggaga
     5941 agaacgggcc ggtgcgtccg cagcccatct ggatgtcgtc gacgatcagc agcatgtcgt
     6001 gccgccggca caggtcggcc aggccgcgca gccactccgg ccgggccacg ttgatgccgc
     6061 cctcgccctg caccgtctcc acgatggtgg cggccggctt gttcaggccg gacccggtgt
     6121 cctccagcag ccgctcgaac cagatgaagt ccggggtctg cccgtcgagg tagtcgtcga
     6181 acggcatcgg cgtgccgtgc accagcggga tgccggcgcc cgcgcgcttg aacgcgttgc
     6241 ccgtgacggc gagcgagccc agggacatgc cgtggaaggc gttggtgaac gagacgatcg
     6301 actcgcggcc cttgaccttg cgggccagct tcagcgcggc ctccaccgcg ttggtgccgg
     6361 tcgggcccgg gaacatgacc ttgtagtcga ggtcgcgggg ccgcaggatc acgttctgaa
     6421 acgattcgag gaaggcccgt ttcgccgtgg tggacatgtc caggccatgg gtgatgccgt
     6481 cgcgctccag gtagtcgagc agggcgcgtt tgagcacggg gttgttgtgt ccgtagttga
     6541 gcgagccggc accggagaag aagtccaggt aggtgtgccc gtcctcgtcg aagaggtagc
     6601 tgccctgggc acggtcgaag acggtgggcc agccgcggca gtagctgcgg acctcggact
     6661 ccagtgtggt gaagacgctc aggtcgggct gggtgatggt cacaacggtc tcctgatctc
     6721 aggggttgat cacgcgggga gtggcggaag cggtgtcggt cgggtcgggt cagcggacgg
     6781 cggccgccgg ctcgggtatc ggcccgatcc ggtacagcac ttccggctgg tggccccgtt
     6841 cggggaacac cccggcgtcg aacagcacct cgcgttccac tgcggccccg tgccgctcgg
     6901 cgaaggatgc gaagagccgg ttggaggcgg tgttgtccgg tgtgacggtc gtttcgatcg
     6961 tgttcacccc cagctcccgg cccgtcctga ggacgagagc gtccagcatc gcggcagcga
     7021 gaccgcgtcc gcgctgaccg ccgtcgacgg cgacctgcca caccagcagg gtgcgcggcg
     7081 cctgagggcg ggtgtacccg gtgatgaagc cgaccggggc gccggcggcg tccctgacga
     7141 cgatcgaggt gcgcgcgtag tcgcgacacc acaggaggta gctgtacgac gagttcaggt
     7201 ccaggaccct ggagtcccgg gcaagacgcc acagggcggc cccgtcgtcc aggcgcgggg
     7261 tgtcgagcgt gagcccttca cgtcctgcgg ggaattcact gtccatgaaa tcgggtcggg
     7321 cacgtgcatg gtctgcttgt gcgggggtca tgcagattta atttacccag caggtcggga
     7381 aattgcatcg ccagtcgggc ttaacagggc gcgccggatg tgttatcgcg cgcgcgtggg
     7441 cggccctctt tgttcggagt gaaaagttac gttctgtccg ttagttaccg ggcaaaacgg
     7501 acagctgtgt ggagtcggtc acatgtccgt aacgaagaag tgacggtgtt ggcccgatcc
     7561 ggtgatgggt cacgatcccg tagatgtccc gtttagacct cgataaagcg ggcagaagag
     7621 gcccgcaagg tccgtcggaa ttacccggga aacgctcgtt gaattcaagt cgaagagcca
     7681 gaagaggcgt cgaagaatta tcccgcggaa gtgtcgaagc cgccactcgc ggcaaccggc
     7741 cccgagggcc cggaccccgt cacccggtac cggtcgaggc ccgcgccgcg ggcccccaga
     7801 tcgtggtcgt actgtgcggc gaacacgtgc tcgcccggcg gcacttcggc ccccggccgc
     7861 agcttccagc ggaacaccag atcaccgtcc tcgttggcca ccgacagatc gaagtcgtcg
     7921 cccggcagcg tccgccatcc cccggtgggc cgcatccccg cctcggccac cacccgcacc
     7981 tcgacggcga acgaggtcag cggctccctc gtcgccaccg tgacgttgct ctgcgaccag
     8041 taggcgtggc tgtccgggtc caccgcgccc gaggaggcga gccgctccgc cgccgacggc
     8101 cgcgccggcc ccgtcggccg cccgtcatcg ctgcccgccc agccgacggc gagcgtcgcg
     8161 agcgccagcg tgaccgcgaa ccccaccgcc gcggccgtca cccggagcct ggccgccctc
     8221 cgccggggcc cggccctcgc tgtcccgtgc tcgacgcgcg ccagcatccg cgcccggtcg
     8281 ggccggtgcg cggccgctgc cgcgtgcagc aactcgcgtg cgtccgggcg gtctcccatg
     8341 gctcctcttc cgcggtcccc tcgctgctca ccaactcctg cgctgcaccg ggcccgtgcg
     8401 cagcgcgcgg aggaactccc cggtcccgcc ctcgccttcg ggggccgccg cctcgacgag
     8461 cagccgttgc aactcggcca tgccccgcga ggtctgactc ttcacggtac ccaccgagat
     8521 ccccagggcg cgcgcggtct cctgctcgga gaggtcgaac gcgtgccgca gcaccacgca
     8581 ggcgcgcttg cggtagggca gccggccgag cgccgcccgc acgtcgagca ccgcgggcac
     8641 gtcgggaccc tccgcgggct caacgcccct ggaccagaac agcgcgatgc ggcggcgctc
     8701 ccgcacccgg ctgcggatac gggatcttgc gagattcgcc accacgccgc gcgcgtacgc
     8761 cagcgggtga tcggccgcgc gcacgcggtc ccagcggtgc cagatcgcga ccagcgcgtc
     8821 ggccgccagg tcgtccgcac cgtcggcgcc ctcggtgccg cccagcaaca ggcgggccag
     8881 ccgggacagt tccgcatggt gctgctcgaa gaaggcgtgg aactcatcgc tggggccgga
     8941 cacgccaacc tccctcggtc aacggctcct gaatccgtcg aagttgttcg acgggacgtg
     9001 cagcgttcgt ctctccgtcc agcccccggc gagcgtagca gtcaatgcgt aagctgccga
     9061 gcatgagtga acgacccgtt ctcccgcccg tactgcacat caggggccgg gtgctggtcg
     9121 gccccgggcc cgacggcgga cccgacctcg acgtgcggga cgaactgtgg gtcgtcggcg
     9181 gccggatcac ctacgaacgc ccgcgcggcg cccgcgaaac cgtccgcctc accggctggg
     9241 tgctgccagg actcgtcgac gcccactgcc acgtcgggct cgacgcccac ggcgccgtcg
     9301 accgggccgc cgccgaaaag caggccctgg ccgaccgggc cgcgggcgcc ctgctgctgc
     9361 gcgacgccgg atcgccggcc gacacccggt ggatcgacgc ccgcaccgac ctgccgcacc
     9421 tcatccgcgc cggccgccac atcgcccgca cccggcgcta catccgcaac tacgcgcagg
     9481 aggtggagcc cgcggacctc gtcgccgccg tccggcacca ggccgcccgc ggcgacggct
     9541 gggtcaagct cgtcggcgac tggatcgacc gcgaacgcgg cgacctggcg ccctgctggc
     9601 ccgccggcgc cctgcgcgcc gcgatcgccg ccgcgcacga ggcgggcgcc cgggtcaccg
     9661 cccactgctt cgccgaggac tcgctgcccg acctggtggc ggccggtatc gactgcgtcg
     9721 agcacgccac cgggctgacc gaggagacca tcccgctgtt cgccgcgcac ggcgtcgcca
     9781 tcgtgccgac cctggtgaac atcgccacct tccccggcct cgccgacggc ggcgaggcca
     9841 agtacccgcg ctggtcggcc cacatgcgcc ggctccacgc ccgcaggtac gacaccgtgc
     9901 gcgccgccca cgacgccggt ctcgacgtct tcacgggcac cgacgcgggc ggcgcgctgc
     9961 cgcacggact catcgcccgg gaggtcgccg agctgcgcac cgccggcatc cccgtccacg
    10021 acgccctcgg cgccgcgacc tggcgggccc gcgactggct gggcaagtcc ggcctgaccg
    10081 agggcgcacc ggccgacctc gtcgtgtacg cggacgaccc gcggcaggac ctcggcatcc
    10141 tggccgatcc ccaacgcgta gtgctgcggg ggcagatcgt cgcctgagtt accccgttcg
    10201 ggtgaagcgg ccccggatcg ggggcgattc ggcacatggg atcgaaggat ggcgggcgtc
    10261 ccagcaacac cttcccgtgg gagttccctg ccgtgaaccg atccgcctgc cgccgctcag
    10321 ccgccgcgct gctcgccgcc gccgccctga cgacccccac cgccacggcc cacgccgacg
    10381 acggccggag cgaggg
//
