LOCUS       NZ_LZNS01000001        11956 bp    DNA     linear   CON 15-JUL-2024
DEFINITION  Streptomyces sp. MP131-18 scaf_01, whole genome shotgun sequence.
ACCESSION   NZ_LZNS01000001
VERSION     NZ_LZNS01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. MP131-18
  ORGANISM  Streptomyces sp. MP131-18
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7861428)
  AUTHORS   Paulus,C., Rebets,Y., Tokovenko,B., Nadmid,S., Terekhova,L.P.,
            Myronovskyi,M., Zotchev,S.B., Rueckert,C., Kalinowski,J. and
            Luzhetskyy,A.
  TITLE     New natural products identified by combined genomics-metabolomics
            profiling of marine Streptomyces sp. MP131-18
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7861428)
  AUTHORS   Tokovenko,B. and Rueckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2016) Helmholtz Institute for Pharmaceutical
            Research Saarland, Helmholtz Centre for Infection Research, Campus
            C2.3, Saarbrucken 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 72x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001984575.1-RS_2024_07_15
            Annotation Date                   :: 07/15/2024 02:52:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,130
            CDSs (total)                      :: 7,069
            Genes (coding)                    :: 6,890
            CDSs (with protein)               :: 6,890
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 62 of 179
            Pseudo Genes (incomplete)         :: 155 of 179
            Pseudo Genes (internal stop)      :: 7 of 179
            Pseudo Genes (multiple problems)  :: 45 of 179
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:18:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6086710
            Orig. end                         :: 6098666
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LZNS01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11956
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="31"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11956
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11956
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6956](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6956)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    861..876
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.22"
                     /tool="antismash"
     misc_feature    1293..1306
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.58"
                     /tool="antismash"
     misc_feature    1673..1688
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.31"
                     /tool="antismash"
     misc_feature    complement(2092..2106)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            complement(2455..4152)
                     /locus_tag="STBA_RS27495"
                     /old_locus_tag="STBA_55680"
     CDS             complement(2455..4152)
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /locus_tag="STBA_RS27495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_55680"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_077061775.1"
                     /sec_met_domain="Pkinase (E-value: 1.6e-38, bitscore:
                     131.2, seeds: 54, tool: rule-based-clusters)"
                     /sec_met_domain="APH (E-value: 8.5e-07, bitscore: 27.8,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGEHRDAAGWAPAPVAPLQSWDPRDVSGYQLQGRLGSGGMGTVYL
                     SRTRGGQPVALKVVHADLAGQPRFRKRFQQEVHAARRVRGRYLAPVVDSNTEGPVPWLA
                     AEYIRGPALSQALNASDGGLDPRTVLRLVAGIASALADIHAAGVIHRDLKPGNVMLAGD
                     GPVVIDFGIARAADATSLTGTRTRVGTPGFMAPEQAVGSEITAATDVFALGLTACVAAA
                     AQHPYGDGPPDAMLYRIAHTEADLDACPAELRGLVGPCLRRAAADRPSAAEVLARCREL
                     AAEWGLDDGPLPAVGWLPFAVRDPAPDAPTLTGPPPPPAAVPQHPDVPRFLSAPVPAPL
                     THGTVSGPGTPPPAAGTRRDSRYLLIAALAVVLVAGLGAGAVLAGVWNGEDDGSGGQVG
                     GEYPADGGTDDKDGAAGGGDQDIQEPSIQEPDTQEPDHGEPDTQEPDHEEPEGGTAAGG
                     IPDFFLGTWTSTPVWDGEEHTRVMEIQQAAPGETAMTLTADGPGYHCEFAADLLTVAGE
                     TVELTASEVTVAEGACQQGDPTVLVPVGDQLRRDNTDGQAPLTYTRTG"
     misc_feature    complement(4263..4280)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.79"
                     /tool="antismash"
     gene            complement(4329..4988)
                     /locus_tag="STBA_RS27500"
                     /old_locus_tag="STBA_55690"
     CDS             complement(4329..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643225.1"
                     /locus_tag="STBA_RS27500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_55690"
                     /product="DUF5825 family protein"
                     /protein_id="WP_077061776.1"
                     /transl_table=11
                     /translation="MDTPPVLRAWRDYDARVLDAPGMYLGDIPLTGSARALVDRLWDLG
                     ARYVEIPALVDLGDPAGPAEPELTVHVLSLVRDLTAQAIYTQWHLRLPAGPRTAPSWMP
                     FSHLHPPATLLGVPDAATHLARWRATHYLGKCQWRRGPGFYQLRDRRFGGLRRYTLTDP
                     AYRSAVDTLIPGAPAEAVPEPVLAALAAEHLAHPVGTHVCWLPYATRRWTQSAMLI"
     gene            complement(5001..6956)
                     /locus_tag="STBA_RS27505"
                     /old_locus_tag="STBA_55700"
     CDS             complement(5001..6956)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993375.1"
                     /locus_tag="STBA_RS27505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_55700"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_077061777.1"
                     /sec_met_domain="TIGR03975 (E-value: 3.9e-176, bitscore:
                     585.6, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLINMPWSPIDLPSLALGILTRSVNEKVPGARASVVHGNLDY
                     FDWLTAQPGFGDFTADDYGYYALSSYFLGCGDWVFSAALYDDPGRRVEEFGRAMRRPLG
                     EERLAQAITLHRGAGGFVRRLAEEVVAQRPDVVGFTSTFQQNTAALAAARAVKQLAPGT
                     VTVMGGANCDSAQGEAVHRNFPFVDYVVRGEGEVAFPALLNALRDSGDLARIPGLCWRD
                     GTGSRVNPMATAPLPPAAIVSPDYAGYLERLAASGARHMIEPKLVIEGARGCWWGEKHH
                     CTFCGLNGSFMQFRSKSPDAFYEEIVGMAREHRVLDMYIVDNILDMNYVTTLLPRLIDS
                     GYDLRMHVEIKANMRREQLQTLADAGLVFVQPGIESLNTRVLGLMDKGVTGCQNVRMLR
                     DGASTGLSVSWNYLYGFPGETEADYEPVIAQLPALEHLNPPTGLSARIAIERFSPYFNR
                     PELGFDELRPAEPYRLTYDLPEHELFDLAYIFDAPPRGITSRTAKQLNQALSTWMTANP
                     TSRLVQHDLGDRIVLLNRRRTFSWTTHVLDDPFELAAFQLLEQPRTPRTLIRKLDERFP
                     RADAAARVPLLLTTWHELGLLFTDAGHHVHVVPTAVNDDMLRLGFMRNVTTAAAERGTD
                     ADTGTVAGRSPAPQPA"
     misc_feature    complement(6985..7000)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 16.83"
                     /tool="antismash"
     gene            complement(7049..7171)
                     /locus_tag="STBA_RS39335"
                     /old_locus_tag="STBA_55710"
     CDS             complement(7049..7171)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="STBA_RS39335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="STBA_55710"
                     /product="hypothetical protein"
                     /protein_id="WP_290443918.1"
                     /transl_table=11
                     /translation="MEKDVIENDPLEHEAEGRSPAVTVTITVPLMSPEDEDLEV"
     gene            complement(7177..8994)
                     /locus_tag="STBA_RS27510"
                     /old_locus_tag="STBA_55720"
     CDS             complement(7177..8994)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958125.1"
                     /locus_tag="STBA_RS27510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_55720"
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="WP_077061778.1"
                     /sec_met_domain="Peptidase_S9 (E-value: 7.9e-32, bitscore:
                     109.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGAGTSSTGTSGADAGQFTVSRPVLPSVCRDNPALMAFVGDARG
                     RCEVFAWHAGRREARQVTDRPAGTVRCAIDPHGVIWWFAERADGTGEWRTQDFAGGPDV
                     PAFADAVPRGRPAGLVLPGGTMAAAGLADDEGLTVRIGRRGAPPRHVLRFPGRARLAGY
                     CPQAGLLAVARAPGDHEAVTVLAPRGRVAAVLSGRGGRVWPLGFAPGHAELLLVREHAD
                     GYQVVTWRADRGLTAHPGCTFDTEITAQWYPRGRRVLIRQERHGRSVLTSADLDRARTR
                     TVPAPPGTVLDATAEADGTVHCLFTDTTTPARVLSFGPTTRHLPPPPPSPPPLDAEVRE
                     RWTDGPDGPVHTLLALPPGRTAPGPVVFLVHGGPARHDKDAYDPAVHSLVASGLAVARV
                     NYRGSTAYGPRWRAAWSRGVGWTQIADLAAVRADLVAAGIADPAAVGLWGTSWGAYLVL
                     LALGRQPALWRAAVAVKPLADYVTAWHLATPALRALDSELFGGTPEQVPERYARSSPLT
                     YVSAVRAPLLVIAANRDAKCPPEQVRSYLNALREAGGRCEELWLDSGHDGLDGADHVRV
                     LRHGLYFLARGLRTPRAGLVPPHRSPERR"
     gene            complement(8991..10271)
                     /locus_tag="STBA_RS27515"
                     /old_locus_tag="STBA_55730"
     CDS             complement(8991..10271)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /locus_tag="STBA_RS27515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_55730"
                     /product="MFS transporter"
                     /protein_id="WP_077061779.1"
                     /transl_table=11
                     /translation="MTGLHSRHTTSSHDFRLLWTASAADSLGTQVSGVVLPLLLLAAGH
                     SPALAGAVVSVSLLAGLAAGPFAAVLADRGARRPLMVCAALTGAAAMGTVAFLATGDRP
                     PLVALCLAVTVERIATCVYAAAAQGWVAAVVAPAGYPRAVSRLQAGEQGALVVGPVLGG
                     ALFHAARWLPFAVDAFTYLLAVVCVRGVRTDLALGTGRGPRWRGAATVVTDLREGLRFV
                     GGRPFLRGVLWWMTGVNGVLAALYYGAVFTLERHGAGTVAIGTVLAVAGAAGVAGALIA
                     PRLARRVSAARLAVLVSWAVVVVAAGLALATGAWAYGALFAMVSLLVPTLAVLLAARAI
                     AVTPPDLQARVGTVLNTVSGIATTAAPLVAGALVAVAGTAGVAVAGAGVMAMVAVYASR
                     VVAPALRASEDGAAAAALPGPAREEAG"
     misc_feature    complement(10155..10157)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(10725..11624)
                     /locus_tag="STBA_RS27520"
                     /old_locus_tag="STBA_55740"
     CDS             complement(10725..11624)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578000.1"
                     /locus_tag="STBA_RS27520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_55740"
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="WP_077061780.1"
                     /transl_table=11
                     /translation="MDAAQIDQLRRFNRAVTARVGVLHDRYLGRDRPVGEARLLWEIGE
                     EGRDVRQLRERLGLDSGYVSRLLRALETGGLVTVETRPGDRRVRTVRLTEAGRAERAAL
                     DGRSDDLARSLLGPLNADQRARLVAAMAEVEHLLTAATVVLEPTDPDHPDARHCLRSYF
                     AEMQACFDTGFDPGHSLLPDAGALRPPHGLFLVARLHGEPVGCAGLKLPPDAPAEIKRM
                     WVAPRARGLGLGRRFLAELETRAARAGHTVLRLDTNKALTAAIGLYHSAGFREVPAFND
                     EPYAHHWFEKHVAAPPPG"
     misc_feature    complement(11622..11636)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.65"
                     /tool="antismash"
ORIGIN
        1 ggcgctggcg gcgtaccggg aggcgcggga gacgctcgtc gaggagctgg gtctggagcc
       61 ggggcccgaa ctgcgggagc tggaacggcg gatgctggcg ggggagccgg tgaacgtcgg
      121 cggggaaggg cagccgcagg tggccgttcc cgcgcagctg cccggcgacg tggccgggtt
      181 cgcggggcgg gaggcggaac tggcggagct ggacgggctg ttgtcgggca ccggtcccgc
      241 ggtcgtctcg gcggtggccg ggacggccgg ggtggggaag acggcgcttg ccgtgcggtg
      301 ggcgcaccgg gtgcgggagc gttttcccga cggccagctg tacgtcgacc tgcgtggtta
      361 cggaccggac cagccggtgg ccccggagga cgcgctgggc gggttcctgc gcgggctggg
      421 cctggacggc gcggcgattc cccaggacac ggccgaacgg gcggcgcggt tccgcacgtt
      481 ggtggaccgg cgccggatgc tcgtggtgct ggacaatgcc cgcagcgtgg agcaggtgcg
      541 accgttgctg cctggcagcc cggcgtgctt cgcgctggtc accagccggg acgcgctggc
      601 cgggctcgtg gcccgcgagg gcgcccaccg ggtcagcctg gaccggctgt cgcgcgggga
      661 agcacgggat ctgctccgcg aggcgctcgg cgcccgggtg gcggccgagc cggcggcgac
      721 cgacgccttg atagagcggt gcgcgcggtt gcccctcgcg ctgcggatca ccgccgagct
      781 ggccaggtcc cggcccgcca gctgcctggc cgatctggcc catgaactcg cggaccagca
      841 gggctcgctg gacctgctgg acgccgacgg cgatccgcat acggcagtgc gcgcggtgtt
      901 ctcctggtcc taccggcggc tggacccggc cgtcgcccgc gtgttccgcc tcctcggtct
      961 gcatccgggg cacgacacgg acgcctacgc ggtcgcggcc atggccggcc tcgggccgcg
     1021 cgaggcgcgc cgcgcgctga cggtactgct gcgggcccac ctggtcgacc aggtctccgg
     1081 cggccggtac cagccgcatg atctgctgcg cgcctacgcc gccgaactcg ccgcggccac
     1141 cgacggcacc gccgcgcgcg aggccgccct gaaccggctg ctggacttct acctgtccac
     1201 cgcctccgcc gccatggacg ccgtcacgcc gtacgagtcc taccggaggc cgaaggtgcc
     1261 cgcctgggga ggcgaggcgc ccccgctcgc ctcgcacgac catgcgctgc gctggctcga
     1321 cgccgaacgc gacaacctgc tggagatcgt caggcacggc gatgcggcct tcaccatcga
     1381 catggcggag accatctggc gctacctgga catcggcggc tacctcgacg aactgatcac
     1441 gctgtccgtc cgcgagctcc aggagtcccg ggccgtcggc aacctgctca cggaggccca
     1501 cgcccgcggc cgcctcggcc tgaccctgag ccggctcggc cgggaccacg aggaggcgat
     1561 cagccatctc cagcagtccc tgaccgccta cgagcaggcc ggggagccgg agttgcaggc
     1621 catcgcgcac aacaacctgg gcatcgcgaa cgtgcggcgg gggaagctgt ccaagggcct
     1681 ggaccacttc gagctggccc tggcgcggaa cggcccgacg gggccgtgga cactgcgccg
     1741 ggcggccacg gtcaatatgg tcgtctccct caagggcctc ggccggtacg aagaggccct
     1801 gggccagatg gaaaacgccc tcgaactctg ccggcgccac ggcgacgaga cgaacgagtc
     1861 gaacgtgcgc gccggtctcg cggagctgtg cgcgctcatg gggcgcgggg agcaggcgcg
     1921 cgaacaggcc acccgcgcct tggccatggc ccggaagacg ggctatcggg gcgtcgaggg
     1981 cgactgtctg cgcctgctcg gcgtcctctg ccgcgagcga ggcgatcacg agcaggcgct
     2041 ggctctgcat caggaggccc tgacgctcgc gcacgacgtg ggcgacgcgg gttcgctcat
     2101 cgagaccagt aacgaactgg cagccaccca cgcggccgcg gggcacccgg cggcggcgct
     2161 gcgcctgtac gacgaggccg tggccctggc caccgaggcc gaccagcgca agatgctcgc
     2221 cgacacgcac gcgggcgccg ccgccgttca cgccggactc ggcgcgcacg acgaggcccg
     2281 cgcgcactgg cgcctggcgc tcggccacta cgaggcactc gacctgcccc aggcggccga
     2341 cgtgcgggcc cgactggccg cggcggaacg ggcggaacgg gcggaacgag cggaacgggc
     2401 tgagcccggc ggggaggtca gcgagtagcg ccgggcgccg cccgggcggg gcggttaccc
     2461 ggtgcgggtg taggtcagtg gggcctggcc gtcggtgttg tcgcggcgga gctggtcgcc
     2521 gacggggacc aggacggtcg ggtcgccctg ctggcaggca ccctcggcca ccgtcacctc
     2581 gctggcggtc agttcgaccg tctccccggc cacggtcagc aggtcggccg cgaactcgca
     2641 gtggtagccc ggcccgtccg cggtgagcgt catcgccgtt tcgccgggcg ccgcctgctg
     2701 gatctccatg acgcgggtgt gctcctcgcc gtcccagacg ggggtggagg tccacgtgcc
     2761 caggaagaag tccggaatcc cgcccgccgc ggtcccgccc tccggctcct cgtggtccgg
     2821 ctcctgggtg tcgggttccc cgtggtccgg ttcctgggtg tccggctcct gaatactggg
     2881 ttcctggatg tcctggtccc cgccgcccgc ggccccgtcc ttgtcgtccg tcccgccgtc
     2941 cgccgggtac tcgccgccga cttggccgcc cgaaccgtcg tcctcaccgt tccagacgcc
     3001 tgcgagcacg gcgccggcgc ccagccccgc gacgagtacc accgcgagcg cggcgatcag
     3061 caggtaacgg ctgtcacgcc gcgtcccggc cgcgggcggc ggcgtgccgg ggcccgacac
     3121 cgtgccatgg gtcagcggcg cggggaccgg cgcggacagg aaccgcggga cgtccggatg
     3181 ctgcggcacc gccgccggcg ggggcggcgg ccccgtgagg gtgggggcgt ccggcgccgg
     3241 gtcccggacg gcgaacggga gccagccgac ggcgggcagc gggccgtcgt ccaggcccca
     3301 ctccgcggcc agctcccggc agcgggccag gacttcggcg gccgagggcc ggtccgccgc
     3361 cgcccggcgg aggcagggcc cgacgagccc gcgcagctcg gccgggcagg cgtcgagatc
     3421 ggcctcggtg tgcgcgatcc ggtagagcat cgcgtccggc gggccgtccc cgtaggggtg
     3481 ttgcgcggcg gcggcgacgc aggccgtcag cccgagggcg aacacgtcgg tggccgcggt
     3541 gatctcgctg cccaccgcct gctccggggc catgaaaccg ggggtgccca cgcgggtcct
     3601 ggtgcccgtc agggaggtgg cgtcggcggc ccgcgcgatg ccgaagtcga tgacgaccgg
     3661 cccgtcgccc gccagcatca cgttccccgg cttgaggtcc cggtggatca cccctgccgc
     3721 gtggatgtcg gccagcgccg aggcgatgcc cgcgacgagc cggagcaccg tgcgcggatc
     3781 gagtccgccg tcgctcgcgt tcagcgcctg ggacagggcg gggccgcgga tgtactcggc
     3841 ggccagccag ggcacggggc cctcggtgtt gctgtccacg acgggcgcga ggtagcggcc
     3901 gcgcacccgg cgggccgcat gcacctcctg ctggaaccgt ttgcggaacc gcggttgccc
     3961 ggcgaggtcc gcgtggacca ccttcagggc gacgggctgc ccgcccctgg tccgcgagag
     4021 gtacaccgtg cccatccccc cggagccgag ccgcccctgg agctggtagc cggatacgtc
     4081 ccgcgggtcc catgactgga gcggtgcgac gggggccggt gcccagcccg ccgcgtcccg
     4141 gtgctccccc atgtccaccc ccgccgtagc ccttcgcttc gtcgcccagc gtgcgtgcgg
     4201 caactcccta tgtatcgtag gctccgtccc gcaccgggag aagcggcccg tgtccgcacc
     4261 gggtaacagc cccgtcacag ccatgtcgcc ccaactgccg cctgccctga gccggttggc
     4321 ggcccgcctc agatgagcat ggcgctctgg gtccagcggc gggtggcgta ggggagccag
     4381 cagacgtgcg taccgacggg gtgggcgagg tgttcggcgg cgagggcggc caggacgggc
     4441 tccggaacgg cctcggccgg ggcgccgggg atgagggtgt ccacggcgct gcggtaggcc
     4501 ggatcggtga gggtgtagcg gcggaggccg ccgaagcggc ggtcgcggag ctggtagaag
     4561 ccgggtccgc ggcgccactg gcacttgccc aggtagtggg tggcgcgcca gcgggcgagg
     4621 tgggtggcgg cgtcggggac gccgagcagg gtggccggcg ggtgcaggtg gctgaagggc
     4681 atccacgagg gagcggtccg cggaccggcg gggagccgga ggtgccactg cgtgtagatg
     4741 gcctgcgcgg tgagatcccg tacgagcgag aggacgtgaa cggtcagctc cggctctgcg
     4801 ggcccggccg ggtcgccgag gtcgacgagg gcggggatct cgacgtaacg cgcgccgagg
     4861 tcccacaggc ggtcgacgag tgcgcgggcg gagcccgtga gggggatgtc accgaggtac
     4921 atgcccgggg cgtcgagcac gcgggcgtcg tagtcgcgcc aggcgcggag cacgggtggg
     4981 gtgtccatgg gtctcccagc tcaggcgggc tgcggtgcgg ggctgcgccc ggcgacggtg
     5041 ccggtgtcgg cgtcggtgcc ccgttccgcg gcggcggtgg tgacgttgcg catgaagccg
     5101 aggcggagca tgtcgtcgtt gacggcggtg gggaccacgt ggacgtggtg gccggcgtcg
     5161 gtgaacagca gcccgagttc gtgccaggtg gtgagcagga gcggaacgcg ggcggcggcg
     5221 tcggcgcgcg ggaaccgttc gtccagcttg cggatcaggg tgcgcggggt gcggggctgt
     5281 tcgagcagct ggaacgcggc caactcgaac gggtcgtcga ggacatgggt ggtccaggag
     5341 aaggtgcggc gccggttgag caggacgatc cggtcgccca ggtcgtgctg gacgaggcgg
     5401 ctggtgggat tggccgtcat ccaggtgctc agggcctggt tgagctgctt cgcggtgcgg
     5461 gaggtgatgc cgcgcggcgg cgcgtcgaag atgtaggcca gatcgaacag ttcgtgctcg
     5521 ggcaggtcgt aggtgagccg gtacggctcg gcggggcgca gctcgtcgaa gccgagttcc
     5581 gggcggttga agtacgggct gaagcgttcg atggcgatgc gcgccgacag gcccgtcggt
     5641 ggattgaggt gttccagggc cggcagctgc gcgatgacgg gctcgtagtc ggcctcggtc
     5701 tccccgggga agccgtagag gtagttccag gagacggaca gcccggtgct ggcgccgtcg
     5761 cggagcatcc gtacgttctg gcacccggtg acgcccttgt ccatcagccc gagcacgcgg
     5821 gtgttgaggc tttcgatgcc cggctggacg aacaccagcc ccgcgtccgc gagggtctgc
     5881 aactgctcgc ggcgcatgtt ggccttgatc tccacgtgca tgcgcaggtc gtagccgctg
     5941 tcgatgagcc gggggagcag ggtcgtcacg tagttcatgt cgaggatgtt gtcgacgatg
     6001 tacatgtcga gcacccggtg ctcgcgggcc atgccgacga tctcttcgta gaacgcgtcg
     6061 ggcgacttgc tgcggaactg catgaacgag ccgttgaggc cgcagaaggt gcagtggtgc
     6121 ttctcgcccc accagcagcc gcgggccccc tcgatcacca gcttgggctc gatcatgtgg
     6181 cgggcgcccg aggcggcgag ccgctccagg tagccggcgt agtcggggga gacgatggcc
     6241 gccggcggca gcggggcggt ggccatgggg ttgacgcggc tgccggtccc gtcgcgccag
     6301 cacaggcccg ggatacgggc cagatcgccg ctgtcgcgca gcgcgttgag gagcgcgggg
     6361 aacgcgacct cgccctcccc gcggacgacg tagtcgacga aggggaagtt gcggtgcacg
     6421 gcttcgccct gcgcgctgtc gcagttggcg ccgcccatga cggtgacggt gccgggggcc
     6481 agctgcttga cggcgcgggc ggcggcgagc gccgcggtgt tctgctggaa cgtggaggtg
     6541 aaccccacga cgtcgggccg ctgggccacg acctcctccg cgagccggcg gacgaaaccg
     6601 cccgcccccc ggtggagggt gatggcctgc gccaggcgct cctcgcccag cgggcggcgc
     6661 atcgccctgc cgaactcctc gacgcgccgg ccgggatcgt cgtacagggc ggcggagaac
     6721 acccagtcgc cgcagccgag gaagtaggag gacagggcgt agtagccgta gtcgtccgcg
     6781 gtgaagtcgc cgaatccggg ctgggcggtg agccagtcga agtagtcgag gttgccgtgg
     6841 acgaccgatg cccgtgcgcc gggcaccttc tcgttcacgc tgcgggtgag gatgcccagg
     6901 gccagcgacg gcaggtcgat gggggaccac ggcatgttga tcagaagcac gcgcacggct
     6961 gctccctccg ggagttgagc tgcggtgagg aaaaactggg gcggggcggg cggggcggcc
     7021 ggcccgtggc cgggccggcc gcggcgtgtc acacctcgag gtcttcgtcc tcggggctca
     7081 tcagcgggac cgtgatggtc acggtgaccg cgggcgagcg gccttcggct tcgtgctcca
     7141 gcgggtcgtt ctcgatgacg tccttctcca tgggggctac ctcctctcgg gggaccggtg
     7201 gggcgggacc aacccggccc gtggtgtgcg cagtccacgg gccaggaagt agaggccgtg
     7261 ccgcagcacg cgcacgtggt cggcaccgtc gaggccgtcg tggccggagt ccagccacag
     7321 ctcctcgcag cgcccgccgg cctcgcgcag cgcgttcagg tagctgcgca cctgttccgg
     7381 cgggcatttg gcgtcccggt tcgcggcgat gaccagcagg ggcgcgcgga ccgccgagac
     7441 gtacgtcagg ggggaggagc gcgcgtagcg ctcgggcacc tgctccgggg taccgccgaa
     7501 cagttcgctg tccagcgcgc gcagcgccgg ggtggccagg tgccaggcgg tgacgtagtc
     7561 ggcgaggggc ttgacggcga ccgcggcccg ccacagcgcg ggttggcgcc ccagcgcgag
     7621 cagcaccagg tacgcgcccc acgaggtgcc ccacaggccc accgccgcgg gatcggcgat
     7681 gcccgcggcg accaggtcgg cgcgcaccgc cgccaggtcg gcgatctgcg tccatcccac
     7741 cccccggctc caggccgcgc gccagcgcgg gccgtaggcg gtggaacccc ggtagttgac
     7801 ccgggcgacc gcgagaccgg acgcgaccag cgagtgaacg gccgggtcgt aggcgtcctt
     7861 gtcgtggcgt gcgggcccgc cgtgcacgag gaacacgacg gggcccggcg cggtgcgccc
     7921 gggcggcagg gcgagcagcg tgtgcaccgg cccgtcgggc ccgtcggtcc accgctcccg
     7981 cacctcggcg tccagcggcg gcggggaggg cggcggcggg ggcagatgcc gggtggtcgg
     8041 gccgaaggag aggacgcggg cgggcgtcgt ggtgtcggtg aacaggcagt gcacggtgcc
     8101 gtcggcctcg gccgtggcgt ccagcaccgt tcccggcggc gccggtaccg tccgggtgcg
     8161 ggcgcggtcc aggtcggccg aggtcaggac gctgcggccg tggcgttcct gccggatgag
     8221 gacccgccgc ccccgcgggt accactgggc ggtgatctcg gtgtcgaacg tgcaccccgg
     8281 gtgggcggtg aggccccggt cggcgcgcca ggtcaccacc tggtagccgt cggcgtgttc
     8341 gcgcaccagc agcagttccg cgtggccggg ggcgaagccg agcggccaga cgcggccccc
     8401 gcggccggac agtaccgcgg cgacccggcc gcgcggggcg aggacggtga ccgcctcatg
     8461 gtccccgggg gcgcgggcga cggccagcag cccggcctgc gggcagtacc cggccagccg
     8521 cgcccggccg gggaagcgca gcacgtgccg gggcggcgcg ccgcgccggc cgatccgcac
     8581 ggtcaggccc tcgtcgtccg caagaccggc cgcggccatc gtcccgccgg ggaggacaag
     8641 accggccggg cggccccgag gcacggcgtc ggcgaacgcg ggaacgtcgg gaccgccggc
     8701 gaagtcctgc gtccgccact ccccggtgcc gtcggcccgt tccgcgaacc accagatcac
     8761 gccgtgcgga tcgatggcgc accgcaccgt tcccgcgggc cggtcggtga cctggcgcgc
     8821 ctcccggcgg cccgcgtgcc aggcgaagac ctcgcagcgc ccccgggcat ccccgacgaa
     8881 cgccatcagc gccgggttgt cacggcagac cgacggcagg accggccggg agacggtgaa
     8941 ctgccccgcg tccgcgccgc tcgtccccgt actgctcgtc cccgcgccgc tcatcccgcc
     9001 tcctcgcgcg ccgggcccgg cagcgccgcc gcggcggcgc cgtcctccga cgcccgcagg
     9061 gcgggggcca ccacccgcga ggcgtacacc gccaccatcg ccatcacccc ggccccggcc
     9121 accgccaccc ccgcggtgcc ggccacggcg accagggcgc cggcgacgag cggcgcggcg
     9181 gtggtcgcga tcccggagac ggtgttgagc accgtgccga cccgggcctg caaatcgggc
     9241 ggggtgaccg cgatggccct ggcggccagc agcacggcca gggtcggcac cagcagcgac
     9301 accatcgcga acagcgcgcc gtaggcccac gccccggtcg cgagagccag ccccgcggcc
     9361 accacgacca ccgcccagga caccagcacc gcgagccgcg cggccgacac ccgccgcgcc
     9421 aggcgcggcg cgatcagcgc cccggccacg cccgccgccc cggccacggc cagcaccgtg
     9481 ccgatcgcca cggtgcctgc gccgtgccgt tccagggtga agaccgctcc gtagtacagg
     9541 gcggccagca ccccgttgac gccggtcatc caccacagca ccccgcgcag gaacggccgg
     9601 ccgccgacga accgcaggcc ctcgcgcagg tcggtgacca ccgtggccgc cccccgccag
     9661 cggggccccc ggccggtgcc gagcgccaga tcggtgcgga ctccgcggac gcacaccacg
     9721 gcgaggaggt aggtgaacgc gtcgacggcg aacggcagcc agcgggcggc gtggaacagc
     9781 gccccgccca gcacggggcc gaccacgagc gccccctgtt cgccggcctg gagccgggag
     9841 accgcccgcg gataccccgc gggcgccacc accgcggcca cccacccctg cgcggcggcg
     9901 gcgtagacgc aggtggcgat ccgttccacg gtcaccgcca ggcacagggc gaccaggggc
     9961 ggccggtcgc ccgtggccag gaacgcgacc gtgcccatgg ccgcggcgcc ggtcagcgcc
    10021 gcgcagacca tcagggggcg gcgcgcgccg cggtcggcca ggacggcggc gaacggtccc
    10081 gcggccagtc cggcgagcag ggacaccgac acgacggcgc cggcgagtgc gggggagtgc
    10141 ccggcggcga ggagtaacag cgggaggacg acgccggaga cctgggtgcc cagggagtcg
    10201 gcggccgacg cggtccacag gagccggaag tcatgtgatg acgtcgtgtg tctgctgtgg
    10261 agacccgtca tgtcacctcc tccgatgtga gagcaccgga tgctaggaga ggtggtggag
    10321 cgccacaagg acgcgcgtag agcagcggcc cggccctcct cgggtgtgtg cagaagttga
    10381 ggggggatgt gaaaaatccg cgttcgggga gggtggcgcg gtcccgcggc gcctccgctg
    10441 cctttgcttc cgtgagccgc tcggcggtga gggggcgcgt gcgccgcatg cggcggtgcg
    10501 ttcgcgccgc ttcggggccg cggggagtgg gggacctgct ggggtccacg gggaacgcga
    10561 ggcggcggcg cgtccgtttc gcccgcaccc ggcctcggcg gcgggcgttc tcgtcagcga
    10621 atcaccgtcc ggtgtgcgcg gggcgggccg cgcggcggcg gttggccgga taacgcgcct
    10681 ccggccaccg ttttgagccg cgcgccccgc gggcgggccg gccgtcaccc cggcggaggt
    10741 gccgcaacgt gcttctcgaa ccagtggtgc gcgtacggct cgtcgttgaa ggcgggcacc
    10801 tcgcggaagc cggcggagtg gtacaggccg atcgcggcgg tgagcgcctt gttggtgtcc
    10861 aggcgcagca cggtgtgccc cgcccgggcg gcccgcgtct ccagctcggc caggaagcgg
    10921 cgccccaggc cgaggccccg cgcgcggggt gccacccaca tgcgcttgat ctccgccggg
    10981 gcgtcgggcg gcagtttcag cccggcgcag ccgacgggct cgccgtgcag ccgggcgacg
    11041 aggaacaggc cgtgcggggg cctgagggcg cccgcgtcgg gcagcaggct gtggcccggg
    11101 tcgaaaccgg tgtcgaagca cgcctgcatt tcggcgaagt acgaccgcag gcagtgccgg
    11161 gcgtcggggt ggtcggggtc ggtgggctcc aggaccacgg tggcggccgt cagcaggtgt
    11221 tcgacctccg ccatggcggc gacgagccgg gcgcgctggt ccgcgttgag cggtccgagc
    11281 agcgaacggg ccaggtcgtc gctgcggccg tcgagcgcgg cgcgctccgc gcggccggcc
    11341 tcggtgagcc gcacggtccg cacccgccgg tcccccggcc gggtctccac ggtcaccagg
    11401 ccgccggtct ccagggcacg gagcaggcgg ctgacgtacc cggagtcgag cccgagccgc
    11461 tcccgcaact gccgcacgtc gcggccctct tcgccgatct cccacagcag cctggcctcg
    11521 ccgacgggcc ggtcgcggcc caggtaacgg tcgtggagca cccccacgcg cgcggtgacg
    11581 gcgcggttga accgccgcaa ctggtcgatc tgcgctgcat ccattctctg actctagtca
    11641 ggtaactttc ggtgtgcgac ccccggcccc gggcccgacc agacggacgc gagcacgcgg
    11701 tcgagtgcct tcgtaccggc tggtgcccag gtcggcttgc cgccggggag gccgcgttcg
    11761 cccgcctggc cgatgcagac gagcatcact ccgagcagca cggcccatcc ccgtgcgcgg
    11821 cggatcgtcg cctcgtccgc gggcgcgtac acctcgaaga agcgggtgtc cgcgcccgcg
    11881 ggcaggagca gccaggcggc cgcgaggtcg gccgccgggt cgccggcgca gagttcgccg
    11941 aagtcgacga tcccgg
//
