LOCUS       NZ_LZNS01000001        10414 bp    DNA     linear   CON 15-JUL-2024
DEFINITION  Streptomyces sp. MP131-18 scaf_01, whole genome shotgun sequence.
ACCESSION   NZ_LZNS01000001
VERSION     NZ_LZNS01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. MP131-18
  ORGANISM  Streptomyces sp. MP131-18
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7861428)
  AUTHORS   Paulus,C., Rebets,Y., Tokovenko,B., Nadmid,S., Terekhova,L.P.,
            Myronovskyi,M., Zotchev,S.B., Rueckert,C., Kalinowski,J. and
            Luzhetskyy,A.
  TITLE     New natural products identified by combined genomics-metabolomics
            profiling of marine Streptomyces sp. MP131-18
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7861428)
  AUTHORS   Tokovenko,B. and Rueckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2016) Helmholtz Institute for Pharmaceutical
            Research Saarland, Helmholtz Centre for Infection Research, Campus
            C2.3, Saarbrucken 66123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 72x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001984575.1-RS_2024_07_15
            Annotation Date                   :: 07/15/2024 02:52:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,130
            CDSs (total)                      :: 7,069
            Genes (coding)                    :: 6,890
            CDSs (with protein)               :: 6,890
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 62 of 179
            Pseudo Genes (incomplete)         :: 155 of 179
            Pseudo Genes (internal stop)      :: 7 of 179
            Pseudo Genes (multiple problems)  :: 45 of 179
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:18:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5873406
            Orig. end                         :: 5883820
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LZNS01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10414
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="29"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10414
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10414
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5414](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5414)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(505..1356)
                     /locus_tag="STBA_RS26505"
                     /old_locus_tag="STBA_53700"
     CDS             complement(505..1356)
                     /EC_number="7.6.2.14"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382992.1"
                     /locus_tag="STBA_RS26505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_53700"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_077061616.1"
                     /transl_table=11
                     /translation="MTTRARVSPPAALARRGRLEVEGLAKTYRGKRRTFEAVRDLTFTV
                     EAGELACVVGPSGAGKTTLLRCIAGLLPPSAGAVRLGGEPVLAPPPGMAVVFQEYARSL
                     FPWLTVAQNVELPLRQKKLPKAARHGLVERSLAAVGLADVPGAHPWQLSGGMQQRVAIA
                     RALAYEPDVLLMDEPFAAVDAQTRAELEDLTRSLWHELRMTVLFVTHDIDEAVYLGEQV
                     LVLSASPTVVLDSVAVDLPARREQLATRALPRFAELRAEVFTLIQRAKTYHTAPPAGEA
                     RA"
     misc_feature    complement(988..990)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1380..2165)
                     /locus_tag="STBA_RS26510"
                     /old_locus_tag="STBA_53710"
     CDS             complement(1380..2165)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382993.1"
                     /locus_tag="STBA_RS26510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_53710"
                     /product="ABC transporter permease"
                     /protein_id="WP_077061617.1"
                     /transl_table=11
                     /translation="MRMLAAGAVRLAYLLGLPAALIALWGAVTAGDRYLYYPPLDEIAA
                     AFGETWFSARLADDVLPSLGRLAAGYSGAVLAGVALGVLLGSRPRLRALSEPALEFCRA
                     LPPPVLIPVLMLVAGIGDTMKITVIVTGAVWPVLLNTVQGVRSVDEILADTARCYGLTG
                     AARLRRLVLPAASPAIVTGMRQALSIALILMVIGEMFASSSGLGFAIVQFQRGFAIPEM
                     WSGIFLLGLVGFGLSLLFGLFESRVLGWYRGLRNARRGE"
     gene            complement(2162..2962)
                     /locus_tag="STBA_RS26515"
                     /old_locus_tag="STBA_53720"
     CDS             complement(2162..2962)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552334.1"
                     /locus_tag="STBA_RS26515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_53720"
                     /product="ABC transporter permease subunit"
                     /protein_id="WP_079252185.1"
                     /transl_table=11
                     /translation="MTWYPHRRIRAVPDGLLGPLGLLLCAGLLEAAPRLGLVPAAYLPP
                     LSETAAALADEAAGGAFWSALADTLRGWGYGLAVAAAAGVGLGLVIGGAAPLRRVTAST
                     IEFLRPIPSVALVPLAVLLYGTGQTSTLLLVVYASVWPVLLQVLAGVRDVDPVARDTAA
                     GFRLSWWLRLRHLVWPTTLPFLMTGLRLAASVALILAVTAQLVIGAPGLGREIARAQAA
                     NAVDRMYALVVVTGLLGVAVNAGARALERRVLFWHASVRKEAAA"
     misc_feature    2936..2953
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 18.77"
                     /tool="antismash"
     gene            complement(2991..3965)
                     /locus_tag="STBA_RS26520"
                     /old_locus_tag="STBA_53730"
     CDS             complement(2991..3965)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840847.1"
                     /locus_tag="STBA_RS26520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_53730"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_077061618.1"
                     /transl_table=11
                     /translation="MLPLRRGHVRAALTAATALLLSASAACGPGGGGSGGDPDDLDLGV
                     IPIIDVAPIRLGIEQGFFAEEGLTVTMTESQGGAAIVPAVTSGSLDIGYSNIVSLLLAR
                     SQDVPVRMVSVGARASADALDDGSGQLMTADPGLTDVADLRGGTVAVNTLLGINEVAVR
                     TGLTRHGLSGEDVSLVEVPIPNMPAALAAGDVDAAMLSEPFITIAEGQGAHAVPVSYAA
                     MGQGLPIAGWFTSESFAAEHPDVVDRFATALERSLRYAEEHPDEARAVLDDYLDLAEGV
                     SARVTLPGWDPRTDAAELEDLARLTAEAGLIENTDPLDGLLAP"
     gene            complement(4055..4975)
                     /gene="griF"
                     /locus_tag="STBA_RS26525"
                     /old_locus_tag="STBA_53740"
     CDS             complement(4055..4975)
                     /EC_number="1.10.3.15"
                     /codon_start=1
                     /gene="griF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515805.1"
                     /locus_tag="STBA_RS26525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_53740"
                     /product="grixazone synthase"
                     /protein_id="WP_079252186.1"
                     /transl_table=11
                     /translation="MVHIRKNHLNMSREEKRRFVHAVREIKRRGIYDRYVDLHIRINSR
                     DYLDKETGKRLGHINPGFLPWHREYLMRFERELQRVDPRVTLPYWDWTTDHGEDSPLWD
                     EEFMGGNGRPGDRRVMTGPFARDNGWVLNISVIPVGDENPALNGNYTHDDRDYLVRDMG
                     TLTDVLPTPRELDETLALPVYDCAPWNHTAGGEAPYDSFRNHLEGYTKFPWEPKVGKLH
                     AAGHVWVGGHMMYIGSPNDPVFFLNHCMIDRCWALWQERNPDVPHYLPLTDTPDVPDLH
                     TPLGPWHTKTPADLIDHTRWYRYDA"
     gene            complement(5001..5414)
                     /locus_tag="STBA_RS26530"
                     /old_locus_tag="STBA_53750"
     CDS             complement(5001..5414)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380458.1"
                     /locus_tag="STBA_RS26530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_53750"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_253195012.1"
                     /sec_met_domain="melC (E-value: 2.9e-29, bitscore: 100.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLNRHLNRREMVAGVAGTALVAAAAATPLVVGDGDADAHAAHGG
                     GEDTGGHQGAGAYVEEYQGRTIRVVPEAEGGGVFIDDRPLHLMKFADDAYLSSMCHYEM
                     APTPLHAARRAVEELRGAALLPSGHHGTHITVV"
     gene            complement(5430..6842)
                     /locus_tag="STBA_RS26535"
                     /old_locus_tag="STBA_53760"
     CDS             complement(5430..6842)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380457.1"
                     /locus_tag="STBA_RS26535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_53760"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_077061619.1"
                     /sec_met_domain="Aldedh (E-value: 8.9e-13, bitscore: 46.2,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGAALPLAAGPPAVAPLAIDPLVSGRSVASADRAPLPSVLGGDLA
                     DVGTAPRLLALAALNEIRDLADGRPVDAGLLAEAARQFAEGTVGGETPGAYVTRVAHAT
                     GLTATAVRQAVHDLVTEIAELPATTAAELPPTGLGDGFATRWVPRGRVFAAVMASNHPT
                     PNAAWVQALFHGYSVLIKPGSRDPFTALRLLRALLAAGLPPAKAAFLPCSRATGEFLLG
                     AADRGIVYGGDQAVRKWQGRASVAVRGPGRTKAVLDADLDDAVLGHLARSASVDGGTRC
                     TNLSAVLTTRPVAQVADRLAERLSALPSLPATDGNATLLVADRARGEQIRARLAELRTE
                     LTDHSARFEPGGTVVPLPDGSVLLRPVVLSADRADHPAVGTELPFPFVVVAPWSPADGI
                     APLRDSLVLNLLTDREELVDAALREPSVRKVTRGTVLPWTAVPGIPHDDNYTQFLLEPK
                     GVVAAREPAARP"
     gene            complement(6842..7900)
                     /locus_tag="STBA_RS26540"
                     /old_locus_tag="STBA_53770"
     CDS             complement(6842..7900)
                     /NRPS_PKS="Domain: AMP-binding (83-301). E-value: 3.7e-12.
                     Score: 37.4. Matches aSDomain:
                     nrpspksdomains_STBA_RS26540_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146189.1"
                     /locus_tag="STBA_RS26540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_53770"
                     /product="AMP-binding protein"
                     /protein_id="WP_077061620.1"
                     /sec_met_domain="AMP-binding (E-value: 7.9e-08, bitscore:
                     29.9, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDATDTAFDAHIRTLMAWHFQEETGSPFWLDKRAGLGFDPVTEV
                     VSAADLTRFPDVSAELRTVPARALIPRALADLPFRVYDSGGTTGSPKRIVDSAARSRML
                     AWARERLLAVGVPAEGNWLHLAPSGPHVIGFDVARYAALGGGIFYTVDLDPRWVKRLLA
                     EGRSDEADAYVQHLLDQAETILETQDVAVLNSTPPLLEAICARPRLHKLVRTQVRAIIW
                     AGTSISAESLRQLNEVFFPDTAIIGIYGNSLMGVAPQRPAAPGDAYPCVFEPHPRTTRL
                     QLVDEAGDLVPYGARGRVRLHLVAEEMFLPNVMERDSAVRIEPSAGGHVDGLADVQTFR
                     ALDDVRIIEGVY"
     aSDomain        complement(6998..7651)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STBA_RS26540_AMP-binding.1"
                     /evalue="3.70E-12"
                     /label="STBA_RS26540_AMP-binding.1"
                     /locus_tag="STBA_RS26540"
                     /protein_end="301"
                     /protein_start="83"
                     /score="37.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="SGGTTGSPKRIVDSAARSRMLAWARERLLAVGVPAEGNWLHLAPS
                     GPHVIGFDVARYAALGGGIFYTVDLDPRWVKRLLAEGRSDEADAYVQHLLDQAETILET
                     QDVAVLNSTPPLLEAICARPRLHKLVRTQVRAIIWAGTSISAESLRQLNEVFFPDTAII
                     GIYGNSLMGVAPQRPAAPGDAYPCVFEPHPRTTRLQLVDEAGDLVPYGARGRVRL"
     misc_feature    7874..7885
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 14.92"
                     /tool="antismash"
     misc_feature    complement(8146..8158)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.54"
                     /tool="antismash"
     misc_feature    complement(8146..8162)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 19.79"
                     /tool="antismash"
     gene            complement(8299..9684)
                     /locus_tag="STBA_RS26545"
                     /old_locus_tag="STBA_53780"
     CDS             complement(8299..9684)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515809.1"
                     /locus_tag="STBA_RS26545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STBA_53780"
                     /product="MFS transporter"
                     /protein_id="WP_077061621.1"
                     /transl_table=11
                     /translation="MSTRSAVSPTAAPPRSGSLSARSAGLRYGALFGPAVFGVTAAGVA
                     LPDVAASLDATPSAVAWVLTAHALALGVGTALFGRLSDSRGLRTSLLLGSLVLAVGAAV
                     CLLAPNLGVLVAGRFVLALGSGAMTSGAVTLSASAEAADRPKVLAGFGATMAVFSASAT
                     LAGGVFTEWVTWRVALVLPALSLIAVPLCLRPAAARQGSGRPLDLPGATLLTVAATSFL
                     LLIQASALEIAAGLVVVLAVALVLSVVLLARRVRTVPASFVPRRLVGDNLYLRTAVTGV
                     GVYAGLFAAMYAVPQILVQEHGWSVLAVGTWLLPGAVVGAVLSRLAGRLPAAGGGSVLL
                     AVIACAMAATLASALGTENAALPVVGASLGFAAFAVTQVVSTALMSARIEPALRGGAMG
                     LLNLTFFVGGGVGSATAGALAKSVSLYTTLGLVAGFPLLAAALALTLTGHGGPRGSKAP
                     QAR"
     misc_feature    10093..10108
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.12"
                     /tool="antismash"
ORIGIN
        1 ccgggtcgcc gggcgtgagg ccgagacggt cggcgaggcc gggctccatc acgaacgtgg
       61 cgtcgacgcc gcagcgttcg cgcccggcca gcacacccgc gtggacggtc ctgttgtgca
      121 gcgcgtcacc gtgtcccgag aagcagaaga cgtgccggaa cccgtcgccc gcaagacccg
      181 cgaacaggtc ggccatgacc tccgtcatca ccgcgggtct gacgctccac gaggcgggga
      241 agcccgcgga gacgacgttg acgccccagt agtacggcgg cacgatcagc gcccgcacgc
      301 cccgttccgc cagcaggacg cggacctcgc gcagccaggc gctcggcagg tagatatcgg
      361 tcccggccgg gaggtgcggg ccgtgctgct cgatcacgcc gaacgcccac agcagcaccg
      421 cgccctcccg ggcggcccgc gccacctcgc cccaggtcag ctcggccacg gtccccgcca
      481 gcacgtcccc gtccggcgcg ggcgtcacgc ccgtgcctcc ccggccggcg gcgcggtgtg
      541 gtaggtcttg gcccgctgga tcagcgtgaa gacctcggcg cgcagttcgg cgaaccgggg
      601 cagggcgcgg gtggccagct gctcgcgccg cgcgggcagg tcgacggcga cgctgtccag
      661 cacgacggtc ggcgaggcgg acagcaccag cacctgttcg ccgaggtaga cggcctcgtc
      721 gatgtcgtgc gtgacgaaca gcaccgtcat gcgcagctcg tgccacaacg accgggtcag
      781 gtcctccagt tcggcgcggg tctgggcatc cacggcggcg aacggctcgt ccatcagcag
      841 cacgtccggc tcgtaggcga gggcgcgggc gatggccacc cgctgctgca tgcccccgga
      901 cagctgccac ggatgggcgc ccggcacgtc ggccaggccg acggccgcga gggaacgttc
      961 gaccaggccg tggcgggcgg ccttcggtaa cttcttctgc cgcagcggca gttcgacgtt
     1021 ctgcgcgacc gtcagccagg ggaacaggct gcgcgcgtac tcctggaaga ccaccgccat
     1081 gccgggcggc ggtgcgagca ccggctcgcc gcccagccgc accgcgcccg cgctcggcgg
     1141 cagcagcccg gcgatgcagc gcagcagcgt ggtcttcccg gcgcccgacg ggccgacgac
     1201 gcaggccagt tcgcccgcct cgacggtgaa cgtcagatcg cgcacggcct cgaaggtccg
     1261 ccgcttcccc cggtacgtct tggccaggcc ctcgacctca aggcgccccc gccgcgcgag
     1321 ggcggcgggc ggcgatacgc gcgctcgggt ggtcatgact ttgctggtca ctctcctcgt
     1381 cactctcctc gtctggcatt ccgcagcccc cggtaccagc cgaggaccct ggactcgaac
     1441 aggccgaaca gcagcgagag accgaacccg acgagcccga gcaggaagat cccgctccac
     1501 atctccggga tggcgaagcc gcgctggaac tgcacgatgg cgaagccgag tccgctggaa
     1561 ctggcgaaca tctcgccgat caccatgagg atcagggcga tggacagcgc ctgccgcatg
     1621 ccggtgacga tcgccgggct cgccgcgggc agcaccaggc ggcgcagccg cgcggccccc
     1681 gtcagcccgt agcagcgcgc cgtgtcggcc aggatctcgt ccaccgacct gacgccctgc
     1741 accgtgttga gcagcaccgg ccacaccgcg ccggtgacga tcaccgtgat cttcatggtg
     1801 tcgccgatgc ccgccaccag catgagcacc gggatcagca ccggcggcgg cagggcgcgg
     1861 cagaactcga gcgccggctc cgacagcgcc cgcagccgcg ggcgggagcc gagcagcacg
     1921 ccgagcgcga cgcccgcgag caccgcgccc gaataaccgg ccgccagccg cccgagactc
     1981 ggcaggacgt cgtcggccag ccgcgcggag aaccatgtct cgccgaacgc ggcggcgatc
     2041 tcgtccagcg gcggatagta gaggtaccgg tcgcccgcgg tcaccgcccc ccacagcgcg
     2101 atcagcgccg cgggcagccc cagcaggtag gcgagccgca cggcgcccgc agccagcatc
     2161 ctcatgccgc cgcctccttc cgcaccgagg cgtgccagaa cagcacgcgc cgttccagtg
     2221 cccgcgcgcc cgcgttgacg gccacgccga gcagcccggt caccacgacc agggcgtaca
     2281 tgcggtccac ggcgttcgcg gcctgggccc gcgcgatctc ccgcccgagc ccgggggcgc
     2341 cgatgaccaa ctgggcggtg acggccagga tcagcgccac cgaggcagcg agccgcagcc
     2401 ccgtcatcag gaacggcagg gtcgtcggcc acaccaggtg acgcagccgc agccaccagg
     2461 acagccggaa gccggccgcc gtgtcccgcg ccaccgggtc gacgtcccgc accccggcca
     2521 gcacctggag cagcaccggc cacaccgagg cgtacaccac gagcagcagc gtggaggtct
     2581 gcccggtgcc gtacagcagg accgcgagcg gcaccagcgc gaccgagggg atcggccgca
     2641 ggaactcgat cgtggaggcc gtgaccctgc gcagcggcgc cgcgccgccg atgaccaggc
     2701 cgagccccac gcccgccgcc gcggcgacgg ccagtccgta cccccagccg cggagcgtgt
     2761 cggccagcgc gctccagaat gcgccgccgg ccgcctcgtc ggccagcgcg gcggccgtct
     2821 cgctcagggg cggcagatac gcggccggaa cgagaccgag ccgcggggcc gcctccagca
     2881 ggcccgcgca cagcagcagg cccagcgggc cgagcagtcc gtctggcacc gcgcggattc
     2941 tgcggtgcgg ataccacgtc atcgcgtcaa ctcccacgtc ggggacgggc tcagggagcc
     3001 agcagcccgt ccagcgggtc cgtgttctcg atcaggcccg cctcggcggt cagccgcgcc
     3061 aggtcctcaa gctccgccgc gtcggtgcgc gggtcccagc cgggcagggt gacccgggcg
     3121 ctcacgccct ccgccagatc caggtagtcg tccaggacgg cccgcgcctc gtcggggtgc
     3181 tcctcggcgt accgcaggga ccgttccagc gccgtcgcga accggtcgac gacgtcgggg
     3241 tgttccgcgg cgaagctctc cgaggtgaac cagccggcga tcggcagccc ctgccccatc
     3301 gccgcgtagc tgacggggac ggcgtgcgcc ccctgccctt cggcgatcgt gatgaacggc
     3361 tcgctgagca tggcggcgtc cacgtcgccg gccgccaggg cggccggcat gttggggatg
     3421 ggcacctcga ccagcgacac gtcctcgccc gagaggccgt ggcgcgtcag gcccgtccgg
     3481 acggcgacct cgttgatgcc gaggagggtg ttgaccgcga ccgttccgcc gcgcaggtcc
     3541 gcgacgtccg tcaggccggg gtccgcggtc atcagctgtc ccgacccgtc gtcgagcgcg
     3601 tccgccgagg cgcgggcgcc caccgacacc atgcgcaccg gaacgtcctg gctcctggcg
     3661 agcagcaggc tgacgatgtt ggagtacccg atgtcgaggc tcccgctggt gaccgcgggc
     3721 acgatggccg cgccgccctg ggattccgtc atggtgacgg tcaggccctc ctccgcgaag
     3781 aagccctgct cgatgccgag ccggatcggg gccacgtcga tgatggggat gaccccgagg
     3841 tcgaggtcgt ccgggtcgcc tccgctgccg ccgccgccgg gaccgcaggc ggcggaggcg
     3901 ctgagcagca gggccgtggc ggccgtgagg gccgcgcgga cgtgtccgcg gcgaagaggc
     3961 agcatggcaa ctccttcggc ttcgggagaa gttggggagc ggtggagccg ggcgggccgc
     4021 gaccggcacg gcccgcccgc cccgccgcgg tgcgctacgc gtcgtagcgg taccaccggg
     4081 tgtggtcgat gaggtcggcc ggcgtcttcg tgtgccaggg ccccagcggc gtgtgcaggt
     4141 ccggcacgtc cggcgtgtcg gtcagcggca ggtagtgcgg cacgtcgggg ttccgttcct
     4201 gccacagtgc ccagcagcgg tcgatcatgc agtggttgag gaagaacacc gggtcgttgg
     4261 gcgagccgat gtacatcatg tggccgccca cccagacgtg cccggcggcg tgcagcttgc
     4321 ccaccttcgg ctcccagggg aacttggtgt agccctccag gtggttgcgg aagctgtcgt
     4381 acggggcctc gccgcccgcg gtgtggttcc acggggcgca gtcgtagacc gggagcgcga
     4441 gcgtctcgtc cagctcccgc ggcgtcggca gcacgtcggt gagggtcccc atgtcgcgca
     4501 ccaggtagtc gcggtcgtcg tgcgtgtagt tgccgttcag cgccgggttc tcgtccccga
     4561 ccgggatcac gctgatgttg agcacccagc cgttgtcgcg ggcgaacggg ccggtcatca
     4621 cccgccggtc gcccggccgc ccgttgccgc ccatgaactc ctcgtcccac agcggcgagt
     4681 cctcgccgtg gtcggtcgtc cagtcccagt aggggagggt gacgcgcggg tcgacgcgct
     4741 gcaactcccg ctcgaagcgc atcaggtact cgcggtgcca gggcaggaag ccggggttga
     4801 tgtggccgag ccgcttcccg gtctccttgt cgaggtagtc ccgcgagttg atccgtatat
     4861 gcaggtccac gtaacggtcg tagatgccgc gccgcttgat ctcccgcacc gcgtgcacga
     4921 agcgccgctt ctcctcgcgg ctcatgttca ggtggttctt gcggatgtgc accatgaggg
     4981 cctccctttc gaccggggcg tcagacgacc gtgatgtggg tgccgtggtg cccggacggc
     5041 agcagcgccg cgccgcgcag ctcctcgacc gcccggcggg cggcgtgcag cggggtgggc
     5101 gccatctcgt agtggcacat ggagctgagg tacgcgtcgt cggcgaactt catcaggtgc
     5161 agcggccggt cgtcgatgaa gacgccgccg ccctcggcct cgggaacgac tctgatggtg
     5221 cggccctggt actcctccac gtacgcgccc gcgccctggt ggccgccggt gtcctcgcca
     5281 ccgccgtggg cggcgtgcgc gtccgcatcg ccgtcgccca ccaccagcgg ggtcgccgcc
     5341 gcggcggcca ccagggccgt tccggcgacg cccgcgacca tttcgcgcct gttcagatgc
     5401 ctgttgagat gcatggggtc aacccttctt caggggcggg cggccggctc gcgcgccgcc
     5461 accacgccct tcggctccag caggaactgg gtgtagttgt cgtcgtgcgg gatgccgggg
     5521 accgcggtcc acggcaggac cgtgccgcgc gtcaccttgc ggacgctcgg ctcgcgcagc
     5581 gccgcgtcga ccagctcctc ccggtccgtc agcaggttga gcaccaggga gtcgcgcagc
     5641 ggcgcgatgc cgtccgccgg tgaccacggc gccaccacga cgaacgggaa cggcagctcg
     5701 gtgccgaccg ccgggtggtc cgcgcggtcc gcggacagca cgacggggcg cagcagtacc
     5761 gagccgtccg gcagcgggac cacggtgccg cccggctcga accgggcgct gtggtcggtg
     5821 agttcggtgc gcagctcggc caggcgggcg cggatctgct cgcccctggc ccggtcggcg
     5881 accagcagcg tggcgttccc gtcggtcgcg ggcagcgagg gcagggcgct gagccgttcg
     5941 gcgagccggt cggcgacctg cgcgaccggg cgggtggtga gcaccgccga caggttggtg
     6001 cagcgggtgc cgccgtcgac cgaggcggag cgcgccaggt ggccgagcac cgcgtcgtcg
     6061 aggtccgcgt ccagcacggc cttggtgcgc ccggggccgc ggacggcgac ggaggcgcgg
     6121 ccctgccact tccgcacggc ctggtcgccg ccgtagacga tgccgcggtc ggccgcgccg
     6181 agcagaaact cgccggtcgc ccgcgaacag ggcaggaacg cggccttcgc gggcggcaga
     6241 ccggcggcga gcagcgcacg cagcagccgc agcgcggtga acgggtcccg gctgccgggc
     6301 ttgatcagca cgctgtagcc gtggaacagg gcctgcaccc aggcggcgtt cggcgtgggg
     6361 tggttgctgg ccatgaccgc ggcgaagacg cgtccgcgcg gcacccagcg ggtggcgaag
     6421 ccgtcgccga gcccggtggg cggcagttcg gccgcggtgg tggcggggag ttccgcgatc
     6481 tcggtgacga ggtcgtgcac ggcctgccgc acggccgtcg ccgtcagccc ggtggcgtgc
     6541 gcgacgcgcg tcacgtacgc gccgggcgtc tcgcccccga ccgtgccctc ggcgaactgc
     6601 cgcgcggcct cggcgaggag gcccgcgtcg acggggcggc cgtccgccag gtccctgatc
     6661 tcgttgagcg cggcgagggc gagcagccgc ggcgcggtgc cgacgtcggc gaggtcgccg
     6721 ccgagcacgc tcggcagcgg cgcgcggtcg gcgctcgcca cggaacggcc gctgacgagg
     6781 gggtcgatgg cgaggggggc gacggcggga gggccggcgg cgagggggag cgcggcgccc
     6841 atcagtagac cccctcgatg atgcgcacgt cgtcaagtgc ccggaaggtc tgcacgtccg
     6901 cgagcccgtc gacgtgcccg cccgcggacg gttcgatccg tacggcgctg tcccgttcca
     6961 tgacgttggg caggaacatt tcctcggcga ccaggtgcag gcgcacccgc ccgcgtgccc
     7021 cgtagggcac gaggtcgccg gcctcgtcga cgagttggag gcgcgtggtc cgcgggtgcg
     7081 gctcgaacac gcaggggtac gcgtcgccgg gcgccgccgg gcgctgcggg gcgacgccca
     7141 tcaggctgtt gccgtagatc ccgatgatcg ccgtgtcggg gaagaagacc tcgttcagct
     7201 ggcgcaggct ctcggcgctg atggaggtgc cggcccagat gatggcccgc acctgtgtcc
     7261 gcaccagctt gtgcagccgg ggcctggcgc agatcgcctc caggaggggc ggggtgctgt
     7321 tgagcaccgc gacgtcctgg gtttcgagga tcgtctccgc ctggtcgagc aggtgctgga
     7381 cgtaggcgtc ggcctcgtcg gaacggccct cggccagcag ccgcttgacc cagcgcgggt
     7441 cgaggtcgac cgtgtagaag atgccgccgc cgagcgccgc gtaccgggcg acgtcgaagc
     7501 cgatcacgtg cgggccgctc ggcgccaggt gcagccagtt gccctcggcc ggcacgccga
     7561 cggcgagcag ccgttccctg gcccacgcga gcatgcggga acgggcggcg ctgtccacga
     7621 tgcgcttggg cgagccggtg gtgccgcccg agtcgtagac gcggaacggc aggtccgcga
     7681 gcgcgcgggg gatcagcgcc cgcgccggta cggtgcgcag ctccgcgctg acgtcgggga
     7741 agcgggtcag gtcggcggcg ctcacgacct cggtgaccgg gtcgaagccg aggccggccc
     7801 gtttgtcgag ccagaacggg gagcccgtct cctcctggaa gtgccacgcc atcaacgtcc
     7861 gtatgtgggc gtcgaaagcg gtgtccgtag cgtctgacat ggcgttctgt ggcctccccg
     7921 tcgcgtgaaa gatgccggtg cgccctatcg tcaggccgcg cgttatcgcg gtcgtatcgc
     7981 ggctgtttcg gcgcggccgg gcggacgcgg gcgccccccg gacggcacgg gccgtccggg
     8041 gggcgcgtcg gggtggctcg gcagccgcgg cgcctagggc ctgtccggtg gatcttcgtg
     8101 gagccagtga gcggcgtccg gtgcgtgcga tcgcgtgggc ccctcttgca cgatgcgtgc
     8161 aagagggagc cggatcgcag ctcgtacttg gccgtactcg cgcgattcgg caacgcagcg
     8221 tgggggcacc tcccagccgc caggctgggg gagtgcgtgc cgggcgtcgc gcgcccgcga
     8281 agatccaccg gacagggcct agcgtgcctg aggcgctttc gagccgcggg ggccgccgtg
     8341 gccggtcagg gtcagggcga gcgcggcggc cagcagcggg aagcccgcca cgaggccgag
     8401 cgtggtgtac agcgagacgg acttggccag ggcgcccgcg gtcgcgctgc cgaccccgcc
     8461 gccgacgaag aaggtgaggt tcagcagccc catcgcgccg ccgcgcaggg ccggttcgat
     8521 acgggcggac atcagcgccg tggacactac ctgggtcacg gcgaaggcgg cgaagccgag
     8581 cgaggcgccg acgaccggca gcgccgcgtt ctccgtgccc agcgcggagg cgagcgtcgc
     8641 cgccatcgcg caggcgatca ccgcgagcag cacgctgccg ccaccggcgg ccggcagccg
     8701 cccggcgagc cgggaaagca ccgcgccgac gacggcgccc ggcagcagcc aggtgcccac
     8761 cgcgagcacg ctccagccgt gctcctgcac caggatctgc ggcaccgcat acatggcggc
     8821 gaacaggccg gcgtacacgc cgaccccggt gacggccgtg cgcaggtaca ggttgtcgcc
     8881 caccaggcgc cggggtacga acgaggccgg cacggtccgc acccggcggg cgagcagcac
     8941 cacggacagc accagggcga ccgcgagcac gacgacgagc ccggccgcga tctccagtgc
     9001 ggacgcctgg atcagcagca ggaacgaggt ggccgccacc gtgagcaggg tggcgcccgg
     9061 caggtcgagc ggtcggccgc tgccctgccg ggcggcggcg gggcgcaggc acagcgggac
     9121 ggcgatcagc gacagggccg gcaggacgag cgcgacccgc caggtcaccc actcggtgaa
     9181 caccccgccg gcgagcgtgg cgctggccga gaacaccgcc atggtggccc cgaacccggc
     9241 gagcaccttg ggccggtcgg cggcctcggc ggacgcggag agcgtgaccg cccccgaggt
     9301 catggccccg gagccgaggg ccagcacgaa gcggccggcc acgagcaccc cgaggttcgg
     9361 ggcgagcagg cagacggccg cgccgacggc cagcaccagc gagccgagca gcagggacgt
     9421 gcgcaggccg cgggagtcgg agagccggcc gaagagggcg gtgccgacgc cgagggcgag
     9481 ggcgtgggcg gtcagcaccc aggcgacggc ggacggggtg gcgtcgagcg aggcggccac
     9541 gtccggcagg gcgacgcccg ccgcggtgac gccgaacacg gcggggccga acaacgcgcc
     9601 gtagcgcagt cccgcggacc tggcgctgag ggagccggaa cggggcgggg cggccgtggg
     9661 ggacacggcg gaacgtgtgg acacgggcag tccttccgcc accggtcggt cggccggcgg
     9721 ctgggcggga tgagcgggag gagccggacg agcggcgggc gcgttgcggc cggggccggg
     9781 agcggcggtc atccggtgcg gcggatggtg tggccgagcc ggaagaggtg gcgcgggtcg
     9841 taggtcgcct tgagggccgc gagccgcgcg taggcggcgg gcgggaagca gtccgccacg
     9901 gcctcggccg tgtcgggtcc ggcgaggaag ttgaggtacc tgcggccggt gctccagggc
     9961 gccatcccgt gcagcacgcg gtcgggcgcg ccggggcggg agagcgtgga gagggtgaac
    10021 gcaccgtcgc ggcccgccac cgcgttgggc acggccgcgg ggcgggagag ggcgccgccg
    10081 aggtggcgca gctcgaccat caggtccgtg gagccggagc cgtccccggc gagcgccagc
    10141 aggcgcgtca ccgcattcgc gtccaactcg cgtaaaacga cgttgcgttc gtggaagggc
    10201 atcggctcgg tcgggtcgcg gtggatgtcg gcgacggccg agtacggcat gtccccgacg
    10261 tcgtcgagga gggcggggcc gatggcccgc agcgggcgga ccaggcgttc gccttccgcg
    10321 ccccggccgt tgaacgcgat gcgcaggtgc gcgaccaggc ggtcgcgcag gaactcgggc
    10381 acggcgggcg cgtccggcag gcgcagcagc gcga
//
