LOCUS       NZ_CP019458            20759 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces autolyticus strain CGMCC0516 chromosome, complete
            genome.
ACCESSION   NZ_CP019458
VERSION     NZ_CP019458.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces autolyticus
  ORGANISM  Streptomyces autolyticus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10029028)
  AUTHORS   Yin,M., Jiang,M., Ren,Z., Dong,Y. and Lu,T.
  TITLE     The complete genome sequence of Streptomyces autolyticus CGMCC 0516,
            the producer of geldanamycin, autolytimycin, reblastatin and
            elaiophylin
  JOURNAL   J Biotechnol 252, 27-31 (2017)
   PUBMED   28472671
REFERENCE   2  (bases 1 to 10029028)
  AUTHORS   Yin,M., Jiang,M. and Lu,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2017) Yunnan University, Yunnan University, 2#
            Cuihu Beilu, Kunming, Yunnan 650091, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 279x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:05:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,227
            CDSs (total)                      :: 8,141
            Genes (coding)                    :: 7,920
            CDSs (with protein)               :: 7,920
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 74 of 221
            Pseudo Genes (incomplete)         :: 182 of 221
            Pseudo Genes (internal stop)      :: 9 of 221
            Pseudo Genes (multiple problems)  :: 42 of 221
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:31:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9261858
            Orig. end                         :: 9282617
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019458.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20759
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hserlactone"
                     /region_number="42"
                     /rules="Autoind_synth"
                     /tool="antismash"
     cand_cluster    1..20759
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="Autoind_synth"
                     /kind="single"
                     /product="hserlactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20759
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10759](-)"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10759)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
     misc_feature    171..186
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.85"
                     /tool="antismash"
     gene            399..1346
                     /locus_tag="BV401_RS38275"
                     /old_locus_tag="BV401_38350"
     CDS             399..1346
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056780.1"
                     /locus_tag="BV401_RS38275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38350"
                     /product="sugar ABC transporter permease"
                     /protein_id="WP_079260789.1"
                     /transl_table=11
                     /translation="MSSKGTAPPSAAAADPPRRTGRRQQRIAAAVLLTPFTALLVAVFL
                     IPVGYAVYLSFFSEDHEGLGFGGGRTVFTGLRSYLSVLEDPSFLSGFGTIALYCLIFVP
                     LVVISALALGLLLDSGLVRLRRTAQMLLYLPHAVPGIIAAVIWLYLYTPGLSPVIKLFA
                     RADITVDFLGLHTVLPSIVNIALWSGLGYNMVIFYAALQALPREVIEAATIDGAGGIRT
                     ALQVKVPIIRGSVVMVCMFSLIGALQLFTEPMLMNQATPMVNSRYTPNMYIYDAAFRRN
                     NYGLASAASVILLIVTCVLSYAVTRWSGRRERRA"
     gene            1346..2260
                     /locus_tag="BV401_RS38280"
                     /old_locus_tag="BV401_38355"
     CDS             1346..2260
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1069: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181530.1"
                     /locus_tag="BV401_RS38280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38355"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_079259865.1"
                     /transl_table=11
                     /translation="MTTTTPTSPVGATRGPSSRPLGKPFGGSRLTSRGVANAVVLIAAL
                     YTMLPALWLLLASTKNADALFGSDIFSLGDFSFARNLSDLFSMDGGLYGEWYVNSLLYA
                     VVGALTGSLISVAAGYAFDKYEFRHKEKFFGLVLTGVMVPPTVLALPLYLVASEIGVVN
                     TFWSVFVPVLFNPFGVYLARLLSSGYVPNEVLEASRVDGAGELQTYVRVSLRMLGPGFV
                     TVFLFQLTAIWNNFFLPMVMLSDQHLYPLSLGLYTWNSAATVSPEYYPLVVIGSLLAVV
                     PLIVAFVLLQRYWKSGLTAGSVK"
     gene            2257..3294
                     /locus_tag="BV401_RS38285"
                     /old_locus_tag="BV401_38360"
     CDS             2257..3294
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056782.1"
                     /locus_tag="BV401_RS38285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38360"
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="WP_087684338.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 2e-45, bitscore:
                     153.2, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPQISARTRTRPRAVLAMAERSARQVLGSVSPDRLTELLDLDP
                     ALVLDDFTTPAAHRALAGAELLVTGWGCPPLDRRTLDAAPRLRAVVHTAGTVRHHITDA
                     CWERGISVSSAAAANALPVAEYTVAMILLANKRVLDIARDYRAERRAIDWNERYPDAGN
                     YRRTVGILSASAIGRRVIELLRPYDLDILLYDPYVTAREAKELGTRRVPLRELFARSDV
                     ISVHTPLLPATEGLVGWELLAAMPDGATLINTARGAVVDQAALTEEVVAGRIRAVLDVT
                     VPEVLPADSPLYDCDNALITPHIAGSKSGELRRLADLAIGEIENYVTGRAFAHPVRPEI
                     LDRSA"
     gene            3704..5647
                     /locus_tag="BV401_RS38290"
                     /old_locus_tag="BV401_38365"
     CDS             3704..5647
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056783.1"
                     /locus_tag="BV401_RS38290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38365"
                     /product="DUF2264 domain-containing protein"
                     /protein_id="WP_079259866.1"
                     /transl_table=11
                     /translation="MPELPFLLPADDRALSPHTGYTRAHWEAAADGMLHAALRYATPGQ
                     ALIDLPGPPSKSGVRSDGLEGFARTFLLAAFRAAGASGQDQHGILERYTAGIARGTLTP
                     GRGDAESWPVIGHHGAHGQPMVESASVALGLRLTAPWTWDALPPDAQDRTEEWLRGALR
                     HQPAPNNWYLFPLTVAGFLESAGRGDAETARAIERGLALLEGWYQGQGWYSDGDGRSFD
                     HYNGWALHMYPLLHAHLAADGALLDRLGPRLRTFLESFSLLFDADGAPIHHGRSLTYRF
                     AAGASVALGALTGHTPLAPGATRRVLSGALRHFLDRGALTEDGVLSLGWYGPHAATLQR
                     YSGPGSPYWASKGFLALLLPPGHPVWTEPEEAAPAEGPDRALALPATGLLIQTTGRDGL
                     VRLHNHGSYKLRPWEAEYAPADPLYARLAYSTRTGPTSADNAPDNHIALEVRGVRSDRL
                     RIHPLAAGPDWLASSHTPAFPDGGPKVPALRIDSLTLVRGRLEVRVHRTVGAPAGTRIH
                     HSGWAVALPPGTPAETERGAEVRLDGGAVTGQLLGLHGWETATAVRAPQGTAYGPWALV
                     PELTGTVGENPSGTLFVALASLTGERDPAPLADLATAEVNGLAVTVRLPDGADVVVDFG
                     TGDAPTVTEVSA"
     gene            5644..6447
                     /locus_tag="BV401_RS38295"
                     /old_locus_tag="BV401_38370"
     CDS             5644..6447
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056784.1"
                     /locus_tag="BV401_RS38295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38370"
                     /product="TIM barrel protein"
                     /protein_id="WP_079259867.1"
                     /transl_table=11
                     /translation="MTRLGICSVTLRGLDPDRVIAAVAGAGLECVEWGGDVHVPPGDED
                     TARWVRDATVDAGLAVASYGSYYRAGHGDPEEFDAVLRSAVALGAPRVRIWAGATGTRE
                     TPPAGRRAVVEDTRRAAALAADAGVELAYEFHRNTLTDGADHALRLLAEVDRAEVATYW
                     QPPVDMPDAEALEGLDALGDRIAAVHAFSWWPGTTRLPLTARAPLWRGVFERLRAHRAA
                     GSGGGPPLDVLLEFVPGDDERLLPREAATLRALAAAGYSPPRPFP"
     gene            complement(6875..7402)
                     /locus_tag="BV401_RS38300"
                     /old_locus_tag="BV401_38375"
     CDS             complement(6875..7402)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BV401_RS38300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BV401_38375"
                     /product="hypothetical protein"
                     /protein_id="WP_079259868.1"
                     /transl_table=11
                     /translation="MYDAADADVPHPVRIADRAPRRRAPRAVAVAAAVGALLALAGCGG
                     GGGGGGKADAKAETGGNAARPSASPAPKAARVEQLASAAGCEPEFTTKVDDYRQAVCKS
                     AKGKFVFLDFVTAKGQRDWLETAQMYGGVYLVGNRWVLSSSPRKNMERLRDEFGGTIEG
                     GTSYGSASGTPQ"
     gene            7630..8937
                     /gene="paaK"
                     /locus_tag="BV401_RS38305"
                     /old_locus_tag="BV401_38380"
     CDS             7630..8937
                     /EC_number="6.2.1.30"
                     /GO_function="GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (87-338). E-value: 3.3e-21.
                     Score: 67.2. Matches aSDomain:
                     nrpspksdomains_BV401_RS38305_AMP-binding.1"
                     /codon_start=1
                     /gene="paaK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056786.1"
                     /locus_tag="BV401_RS38305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38380"
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="WP_079259869.1"
                     /sec_met_domain="AMP-binding (E-value: 9.3e-26, bitscore:
                     89.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGSPSAAPLDDAERMSPEELRALQLTRLRATLRHAYDHVELYRK
                     KFREAGVGPEDCRTLEDLARFPFTTKADLRDSYPFGMFAVEQSEVRRIHASSGTTGRPT
                     LVGYTEHDLAVWAEVIARSIRAAGGRPGHRVHVSYGYGLFTGGLGAHYGAERLGCTVIP
                     ASGGMTARQVRLIQDLRPEIIMITPSYLLTLLDEFERQGVDPRSTSLQVGIFGAEPWTE
                     EMRREIEERMGIHAVDIYGLSEVIGPGVAQECVETKDGLHVWEDHFYPEIVDPLTDAVL
                     PGGEEGELTFTTLTKEALPVIRYRTRDLTRLLPGTARPAFRRIQKITGRCDDMLIVRGV
                     NVFPSQIEEIVLRVPAVAPHFQVELTRRGRMDHMAVRIEARPGAEPEQRAAAAAAIVRG
                     VKDGVGLTVEVEIVDPETLERSVGKIRRVKDLRTEG"
     aSDomain        7891..8643
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BV401_RS38305_AMP-binding.1"
                     /evalue="3.30E-21"
                     /label="BV401_RS38305_AMP-binding.1"
                     /locus_tag="BV401_RS38305"
                     /protein_end="338"
                     /protein_start="87"
                     /score="67.2"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="SEVRRIHASSGTTGRPTLVGYTEHDLAVWAEVIARSIRAAGGRPG
                     HRVHVSYGYGLFTGGLGAHYGAERLGCTVIPASGGMTARQVRLIQDLRPEIIMITPSYL
                     LTLLDEFERQGVDPRSTSLQVGIFGAEPWTEEMRREIEERMGIHAVDIYGLSEVIGPGV
                     AQECVETKDGLHVWEDHFYPEIVDPLTDAVLPGGEEGELTFTTLTKEALPVIRYRTRDL
                     TRLLPGTARPAFRRIQKITGRCDDMLIVR"
     CDS_motif       8611..8673
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BV401_RS38305_0001"
                     /evalue="3.00E-04"
                     /label="NRPS-A_a8"
                     /locus_tag="BV401_RS38305"
                     /protein_end="348"
                     /protein_start="327"
                     /score="13.6"
                     /tool="antismash"
                     /translation="TGRCDDMLIVRGVNVFPSQIE"
     gene            complement(9147..10004)
                     /locus_tag="BV401_RS38310"
                     /old_locus_tag="BV401_38385"
     CDS             complement(9147..10004)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056787.1"
                     /locus_tag="BV401_RS38310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38385"
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="WP_087684339.1"
                     /transl_table=11
                     /translation="MTVPAAMSLPAHAWAAWSPCTTGCVAHAAGRVPATRAARRAAVFG
                     RSVMGALLSGQKIAEPERLRARAAALLGSLGIRLEVTGDTGLSAPGGTGTLVVANHISW
                     LDVIALLAIEPVVMLAKREIAGWPLIGPMVTRAGTMYIDRGSLRELPETVREMAARLRD
                     GQSVMAFPQGITWCAGTGGSFRRATFQAALDAGAPVRPVTLDYVQHGAPTTVAAFVGED
                     DFGASLRRVLRADGLTVRVDLHRPLRPMQAVDDRRRVAAQAQATVCGARLPLHHEVAAL
                     ELAR"
     gene            complement(10001..10759)
                     /locus_tag="BV401_RS38315"
                     /old_locus_tag="BV401_38390"
     CDS             complement(10001..10759)
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hserlactone: Autoind_synth"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056788.1"
                     /locus_tag="BV401_RS38315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38390"
                     /product="GNAT family N-acyltransferase"
                     /protein_id="WP_069867263.1"
                     /sec_met_domain="Autoind_synth (E-value: 8.5e-05, bitscore:
                     21.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVLTTSSTTAATSSYVTSIAHTPEQIHAAQRLRYQVFGEERGGR
                     LDAAVDGRDVDEFDEVMDHLIVTDTATGTVVGTYRLLPPGRSERLYSDTEFDLRGLPAE
                     VRSSMVEAGRACVHADHRSGAVINLMWAGLARYVLLSGHRYLAGCASVPLADGEQAAAG
                     AWLLGTTKHAAPPEMRVHPHDPWIPSRPLDGEPSYAELPPLLRGYLRVGAWMCGSPQHD
                     RAFNDADFFVLLDTERMNDRYRRYFLGESQ"
     gene            complement(10972..11664)
                     /locus_tag="BV401_RS38320"
                     /old_locus_tag="BV401_38395"
     CDS             complement(10972..11664)
                     /EC_number="3.5.1.88"
                     /GO_function="GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /GO_process="GO:0018206 - peptidyl-methionine modification
                     [Evidence IEA]"
                     /GO_process="GO:0031365 - N-terminal protein amino acid
                     modification [Evidence IEA]"
                     /GO_process="GO:0043686 - co-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1275: peptide deformylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056789.1"
                     /locus_tag="BV401_RS38320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38395"
                     /product="peptide deformylase"
                     /protein_id="WP_079259870.1"
                     /transl_table=11
                     /translation="MPTTSLSELVDELLSGIRPLPIVSAGVPVLRRPALPYDGQLAPDQ
                     LDRLLAAMRETMHSAPGVGLAAPQIGVPLRLAVIEDPAEVTAEVREARGRVPQPYRVLV
                     NPVYEPIGDGRAAFFEGCLSIPGWAAVVSRPDRIRLRGQDETGRELDEEFTGWPARIVQ
                     HETDHLGGVLYLDLAETRSLSTAQAVSDYWSQPTPATAAHALGFPLRSATGATDAADDS
                     GDTAATAP"
     gene            complement(11881..12459)
                     /locus_tag="BV401_RS38325"
                     /old_locus_tag="BV401_38400"
     CDS             complement(11881..12459)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712820.1"
                     /locus_tag="BV401_RS38325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38400"
                     /product="DUF2267 domain-containing protein"
                     /protein_id="WP_335755879.1"
                     /transl_table=11
                     /translation="MRYEELTGLVQARAQLPDRQSAERAIRATLETLAERIPGGLADHM
                     AAQLPHEAGEPLRRVAAAHEGTPEERTYRRDHGERFGLTGFAGRIAWRTEHTEGEALRE
                     AAAFFEVLDSAVDPELMEKLYGVLPNDIRGLLPEARAEQDARAERDTRTERGSRTERGS
                     RTERDSRTERDSRTERDSRTERGSQAERG"
     gene            complement(12724..13227)
                     /locus_tag="BV401_RS38330"
                     /old_locus_tag="BV401_38405"
     CDS             complement(12724..13227)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056793.1"
                     /locus_tag="BV401_RS38330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38405"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_069867260.1"
                     /transl_table=11
                     /translation="MTRTPLADVACSIARATDLFGDAWTALIMRDVLTGVTRFDDLAHD
                     LGISRKVLAARLARLVEEGVLIRERYQERPPREHYRATAKGEELYPVLLALMEWGDRWY
                     AGSAGPPARIRHLGCGRDTTPVSACAHCGEPLTVGNTTQLPGPGGRTGPGTRVLGPLLA
                     AREG"
     gene            13364..14131
                     /locus_tag="BV401_RS38335"
                     /old_locus_tag="BV401_38410"
     CDS             13364..14131
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181659.1"
                     /locus_tag="BV401_RS38335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38410"
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_079259871.1"
                     /transl_table=11
                     /translation="MAAPHIELDSRSPEAVDGRQIRAVTFQDSRLEYVRGTLGNVDLPA
                     PGSRAVVVGSGRGLLARGLAGLGFEVVAVDPSAAATEMAREADGGEHPGVDYRTAPAER
                     LGLADGAFDLAYYADTFEITSELDLVLAEAARVLRPGGVLIYDTVNRTFLSRLIYLGAF
                     QRVPMTRIMPAGRYTAARLRTPAELTAALERQGLRNEDICDFKPKDPRSLVKATMARRR
                     GRISDEQIPPIVDFVLAPDARPLVTYLGYARKG"
     gene            14203..14706
                     /locus_tag="BV401_RS38340"
                     /old_locus_tag="BV401_38415"
     CDS             14203..14706
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539947.1"
                     /locus_tag="BV401_RS38340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38415"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_193525021.1"
                     /transl_table=11
                     /translation="MTHKHSAGLLLYRGAVPAGPAVEVLLAHMGGPFWARKDEGAWTVP
                     KGEYEEDETPEAAARREFQEELGVPPPGGEPIPLGSVTQTGGKVVIVWALRGELDPAEI
                     TPGTFAMEWPKGSGRMREFPEIDRVAWFGLDEARRKIVTRQRIFLDRLEELLQEAAGAG
                     PGGA"
     gene            complement(14734..19371)
                     /locus_tag="BV401_RS38345"
                     /old_locus_tag="BV401_38420"
     CDS             complement(14734..19371)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181661.1"
                     /locus_tag="BV401_RS38345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38420"
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="WP_079260792.1"
                     /transl_table=11
                     /translation="MLRRLRRRLPLPRFRHPLRFSRLFSRLSRFPRLAALALALALTVP
                     TAPAAAADPKDPPADVYQLLEDPEVTSVGQEPAHARLTPYADTAEALGGGSRSRWTASL
                     DGTWKLHMSDRPEEVPKDFFTEGYDTSEGGWRSVSVPHTWQTDGLDHPMFRNIPTEMYP
                     DDPPKVPHDVNPTGAYVKTFELPESWEKRRTFLRFEGVTSGYLVWVNGSYAGYDQGGYT
                     PAEFDISDRLHPGRNTVAVQVHRWGSGSHLEDYDQWRFSGIFRSVGLYSTPATYLRDIT
                     VKTDLDARYRDARLTAEVDIAAKGPKTPDRAPDGRKVLGTLYDQRGHKVTTMSGTVDGD
                     SGSTVLTAHVANPAKWTDETPHLYTLVVRLTDADGTVTHTTAQPVGFREIEIKDRQLLV
                     NGERILVKGVNRSETDPDTGRHATRERTASDVALMKRLNINSVRTSHYPSDPYLYELAD
                     ARGLWIDDEVDIETHHHDACPDNCLAERPEWQAAFLDRLMAMYERDKNHPSVLMWDTGN
                     EAGLGKAHYAMADFLDREDPARPVYHQPNSPDGDAPFADVWGPRYPSPASLADKAKTTT
                     KPIIMGEYAHAMGNSLGNFREFWDVVRKYPQVQGGYIWDWAEQNITQPLLTTPDTSGND
                     ILSYVSGKPGLVAGHRGRALELSGLDDFVEVYRDPKLDAVSDALTLDAWVKPADWTGSF
                     TVIAKGDHSYALKMRDKDTLEFFVHGDGDWHTVAADVPDGWYGSWHRVSGTFDGRTLRL
                     LIDGKQTASADWTGSVDHSAQPVNIGRNPETEQENIGTRMAHGTVDQVRVYHKALSPDQ
                     LAADPRADAVLALDFDRLDRKGEFLSYGAGTGGVDGVVSSDRTVQPEARAMAAVHAPIR
                     ISGDEARAGRVEVRNERSFTGTDDLRLRWRVTEGARTLTRGSRTLDLGPGQHTTLQLPK
                     PPANPRDADRQLTVEAVWASDTAWAKAGERVAVEQFDIGGRQLAGTVPARAPGKVRATT
                     SGGRLTVTGEGFSYGFDRTSGELVSMKADGRELLGSGPELDAWRAPVSNEIGSEEGPWR
                     DAGLDRLRTEPGKVTVAERGGEVVVTVPSSAAAPGVRDSSFAQTLRYTVSGTGELRLDH
                     RVEARGAARAVPYLPRLGLSLRVPDRYDRFTWYGRGPQENYNDREDGAPVGVYSTDVDE
                     QFAGYTKPQDYGNHEDVRWASLSDGSSGLLVSGDFSAGVTPYTGIDRAAYPFALREDPE
                     GNTLHLDHAVSGVSETFHTVLPEYQVRPAKEYAYTLRLRPLTGAEARTGTPHGPVVCAP
                     EAKLTPADTTVPAGGSTSAELTVTNPCDTPLREVTAAFGLPEGWTATPGTLDLGDLAAG
                     RTATVRTELTRGEGTPNGPRPAVAEVRATSTGGARVSGSASAEIDGTPPPPRGEVSVST
                     LDFLTADNGWGPVERDRSNGEDAPGDGKTLTIDGTTYERGLGTHADSTVEVFLGGNCST
                     FTATTGLDDEVGADGSVVFEVYADGERVYRGETAHGSDAAVPVEARIEGARKLRLRVTD
                     GGDGNAHDHADWAAATVRCGTGGTG"
     misc_feature    complement(19813..19827)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
     misc_feature    complement(20216..20231)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.15"
                     /tool="antismash"
ORIGIN
        1 ctgaaggcca aactcgccag cggtgtcagc agcgccttcc cttccgtgcc cggtctggtc
       61 ccggtcgccc gtaaggagat ggacaccagc tactactccg gccaggacat cttcggcctc
      121 ttcgaggaac aggccgagat gatctccccc acctggaagt gggggccgcg gatggtctcg
      181 accaccacct ccggcgacga cgggctcgcc cgcgccggag ccggcagcgg caccatcctg
      241 gaggccctgc gcgaggccca gagcagaacc atgcccgatc tgaagagcct cggcctgtcg
      301 gtcaccaccc gctgagccgc cacgcccccc ccgcatctcc gccccacccc gagccgcacc
      361 tcatcccgcc tgagccgagg taacccgtga gcaccgtcat gtcctcgaaa gggacggcgc
      421 cgccatcggc ggccgccgcc gacccgcccc gcagaaccgg acgacgccag cagcggatcg
      481 ccgccgccgt tctgctgacc cccttcacgg cactgctcgt cgccgtgttc ctgatccccg
      541 tcggctacgc cgtctacctc agcttcttct ccgaggacca tgagggactc ggcttcggcg
      601 gcgggcgtac ggtcttcacc ggactgcgca gctatctctc ggtgctggag gaccccagct
      661 tcctctccgg gttcggcacc atcgcactct actgcctgat cttcgtcccg ctcgtggtca
      721 tcagcgcgct cgcgctcggc ctgctgctcg actcgggcct ggtccggctg cggcgcaccg
      781 cacagatgct gctctatctg ccgcatgccg tgcccggcat catcgcggcc gtcatctggc
      841 tgtacctcta caccccgggg ctcagcccgg tcatcaaact cttcgcccgg gccgacatca
      901 ccgtcgactt cctgggcctg cacaccgtgc tcccgtcgat cgtcaacatc gccctgtgga
      961 gcggcctcgg ctacaacatg gtcatcttct acgccgcgct ccaggcgctg ccgcgcgagg
     1021 tgatcgaggc ggcgaccatc gacggcgccg gcggcatccg caccgcgctc caggtcaagg
     1081 tgcccatcat ccggggctcc gtggtgatgg tgtgcatgtt ctccctgatc ggcgcgctcc
     1141 agctgttcac cgagcccatg ctgatgaacc aggccacccc gatggtcaac tcccgctaca
     1201 ccccgaacat gtacatctac gacgcggcct tccgccgcaa caactacgga ctcgcctccg
     1261 cggcctccgt catcctcctg atcgtcacct gcgtgctgtc gtacgccgtg acgcgctggt
     1321 cgggccgccg ggaacggaga gcgtgatgac cacgaccaca cccaccagcc ccgtgggcgc
     1381 cacccggggc ccgtcgtcca gaccgctcgg caagcccttc ggcggctcca ggctgaccag
     1441 ccgcggcgtc gccaacgccg tggtcctgat cgccgccctc tacaccatgc tgcccgcgct
     1501 gtggctgctg ctggcgtcca ccaagaacgc cgacgccctc ttcggcagcg acatcttctc
     1561 cctcggcgac ttctccttcg cccgcaacct ctccgatctg ttctccatgg acggcgggct
     1621 ctacggcgag tggtacgtca acagcctgct gtacgcggtc gtcggcgccc tgaccggctc
     1681 gctgatcagc gtggccgcgg gctacgcctt cgacaagtac gagttccggc acaaggagaa
     1741 gttcttcggg ctcgtcctca ccggcgtcat ggtgccgccg accgtactgg cccttccgct
     1801 ctatctggtg gcctccgaga tcggggtggt caacaccttc tggtcggtct tcgtcccggt
     1861 gctcttcaac cccttcggcg tctacctcgc ccgcctgctc agcagcggct atgtgcccaa
     1921 cgaggtgctg gaggcgtccc gggtcgacgg cgcgggcgag ttgcagacct atgtacgggt
     1981 cagcctgcgg atgctcgggc ccggtttcgt gaccgtcttc ctcttccagc tcacggccat
     2041 ctggaacaac ttcttccttc ccatggtgat gctctccgac cagcacctct atccgctcag
     2101 cctcggcctc tacacctgga acagcgccgc caccgtctcg cccgagtact accccctcgt
     2161 ggtcatcggc tcactgctcg ccgtcgtgcc gctgatcgtg gcgttcgtcc tgttgcagcg
     2221 ctactggaag tccggactga ccgcagggag cgtcaagtga ccgcaccgca aatctccgcc
     2281 cgcacccgca cccgcccccg tgccgtcctg gccatggccg aacggtccgc ccggcaggtg
     2341 ctcgggtccg tgagcccgga ccggctcacc gaactcctcg acctcgaccc cgccctcgtc
     2401 ctggacgact tcaccacccc ggccgcgcac cgggccctcg ccggggccga actgctcgtc
     2461 accggctggg gctgcccgcc cctggaccgc cgcacgctcg acgccgcgcc ccggctgcgc
     2521 gccgtggtcc acaccgcggg caccgtacgc caccacatca ccgacgcctg ctgggagcgc
     2581 ggcatctcgg tctcctcggc ggccgccgcc aacgccctgc ccgtggccga gtacaccgtc
     2641 gccatgatcc tgctggccaa caagcgcgtc ctggacatcg cccgggacta ccgcgccgag
     2701 cggcgcgcga tcgactggaa cgagcgctac cccgacgccg gcaactaccg ccggacggtg
     2761 ggcatcctca gcgcctccgc catcggccgc cgggtgatcg agctgctgcg cccgtacgac
     2821 ctcgacatcc tgctgtacga cccgtatgtc acggcccggg aggcgaagga gctcgggacg
     2881 cggcgggtgc ccctgcggga actcttcgcc cgcagcgatg tcatcagcgt ccacaccccg
     2941 ctgctgcccg ccaccgaggg gctggtcggc tgggagctgc tggccgccat gccggacggc
     3001 gccacgctca tcaacaccgc gcgcggggcg gtggtggacc aggcggcgct gaccgaggag
     3061 gtggtggccg ggcggatccg cgccgtcctc gatgtcaccg tgccggaggt gctgcccgcc
     3121 gactccccgc tgtacgactg cgacaacgcg ctgatcaccc cgcatatcgc cgggtccaag
     3181 agcggtgagc tgcgccgcct ggccgacctc gccatcggcg agatcgagaa ctacgtcacc
     3241 ggccgcgcct tcgcccaccc cgtacgaccg gagatcctcg accgctcggc ataaggggtc
     3301 cttgcactat gagcgcccga tgaggtcgcg tggtctggtg cggggggcac ctcccagcgg
     3361 tagctgggag agcatcgcaa ggcgccggag agccctcgag cggagctacc agggcttttc
     3421 ggcaacgcgg cgaggtgcgg tgccaggccc cgcgacccgg gcgggcatag tgcaaggacc
     3481 cctaagaggg cgtgcggaca ggcggggcgg cgtcgcgggg cgatgggcgg cgcccggcgg
     3541 cgttgccgtc ggtcgcaggc tcccccggcc accgccggga ggtgcccctc cgcgtcgccc
     3601 cctcctccgc ctcgccccgc tcgtcccccg ccccgcccct tctcccaccc cacccatccg
     3661 cgcgccctct cacgttccgc gaccccagga ggcccacccc gccatgcccg agctcccctt
     3721 cctcctcccc gccgacgacc gcgccctcag cccccacacc ggctacaccc gcgcccactg
     3781 ggaggccgcc gcggacggca tgctgcacgc cgccctccgt tatgccaccc ccggccaggc
     3841 cctgatcgac ctgcccggcc ccccgtccaa gtccggggtg cgctccgacg ggctcgaagg
     3901 gttcgcccgc accttcctcc tcgccgcctt ccgcgccgcc ggggcctcgg gccaggacca
     3961 gcacggcatc ctggagcgct acaccgccgg gatcgcccgc ggcaccctca ccccagggcg
     4021 cggcgacgcc gagtcctggc cggtgatcgg ccatcacggc gcccacggcc agcccatggt
     4081 ggagtccgcg tcggtggccc tgggcctgcg gctgaccgcg ccgtggacct gggacgccct
     4141 gccgcccgac gcccaggacc gcaccgagga gtggctgcgc ggcgcgctgc gccaccagcc
     4201 cgcccccaac aactggtacc tcttccccct caccgtggcc ggattcctgg agtccgccgg
     4261 gcgcggcgac gccgagacgg cccgcgccat cgagcgcgga ctcgccctcc tggagggctg
     4321 gtaccagggc cagggctggt actccgacgg cgacgggcgg tcgttcgacc actacaacgg
     4381 ctgggcgctg cacatgtacc cgctgctgca cgcccatctc gccgccgacg gcgcgctcct
     4441 ggaccggctc ggcccccggc tgcgcacctt cctggagagc ttctcgctgc tcttcgacgc
     4501 cgacggcgcc cccatccacc acggccgctc cctcacctac cgcttcgccg ccggcgcctc
     4561 ggtggccctc ggcgcgctca ccggccacac ccccctcgcg cccggggcca cccgccgcgt
     4621 cctcagcggg gcgctgcgcc acttcctgga ccgcggggcg ctgaccgagg acggggtgct
     4681 gagccttggc tggtacgggc cgcacgccgc gaccctccag cgctactccg ggcccggctc
     4741 gccctactgg gcctccaagg ggttcctcgc cctgctgctc ccgcccggcc acccggtgtg
     4801 gaccgagccc gaggaggccg cgcccgccga gggtcccgac cgggcgctgg cactgcccgc
     4861 caccgggctc ctcatccaga ccacgggccg ggacgggctg gtgcggctgc acaaccacgg
     4921 cagctacaag ctccgcccct gggaggccga gtacgccccc gccgacccgc tctacgcccg
     4981 gctcgcctac tccacccgta ccgggcccac cagcgcggac aacgcccccg acaaccacat
     5041 cgccctcgaa gtgcgcggag tgcgcagcga ccggctgcgc atccacccgc tggccgcggg
     5101 ccccgactgg ctcgcctcct cccacacccc cgccttcccc gacggcggcc ccaaggtgcc
     5161 cgcgctccgc atcgacagcc tgaccctcgt ccgcggccgg ctcgaagtgc gcgtccaccg
     5221 caccgtgggc gcccccgccg ggacccggat ccaccacagc gggtgggccg tcgccctgcc
     5281 cccgggcact ccggcggaga ccgagcgggg cgccgaggtg cggctggacg ggggagcggt
     5341 gaccggtcaa ctcctcggac tgcacggctg ggagaccgcc accgcggtcc gggcgccgca
     5401 gggcaccgcg tacgggccgt gggccctcgt ccccgaactc accggcaccg tcggcgagaa
     5461 cccctccggc accctcttcg tcgccctggc ctcgctcacc ggggaacgcg acccggcccc
     5521 cctcgccgac ctcgccaccg ccgaggtgaa cgggctcgcg gtcaccgtgc ggctgccgga
     5581 cggcgcggac gtcgtggtgg acttcggcac gggcgacgcg cccaccgtca ccgaggtgag
     5641 cgcatgaccc ggctcggcat ctgctcggtg accctgcgcg gactcgatcc ggaccgggtg
     5701 atcgcggcgg tggccggagc ggggctggag tgcgtcgaat ggggcggcga tgtccatgtg
     5761 ccgcccggcg acgaggacac cgcgcggtgg gtccgggacg ccaccgtgga cgccgggctc
     5821 gccgtcgcct cgtacggctc ctactaccgc gccgggcacg gcgatccgga ggagttcgac
     5881 gcggtgctcc gctccgccgt ggcgctcggc gcaccgcgcg tccggatctg ggcgggcgcc
     5941 accggcaccc gggagacccc gccagccggg cgccgcgcgg ttgtcgagga cacccggcgc
     6001 gcggccgcgc tcgccgcgga cgcgggggtg gaactcgctt atgagttcca ccgcaacacc
     6061 ctgacggacg gcgcggacca cgccctccgg ctgctggcgg aggtggaccg cgccgaggtc
     6121 gccacctact ggcagccgcc cgtcgacatg cccgacgccg aggccctgga ggggctggac
     6181 gcgctgggcg accggatcgc cgcggtgcac gccttctcct ggtggcccgg gaccacccgg
     6241 ctgccgctga ccgcccgcgc cccgctgtgg cgcggcgtct tcgagcgact gcgcgcacac
     6301 cgcgccgcgg ggtccggcgg cggtccgccg ctggatgtcc tgctggagtt cgtgccgggc
     6361 gacgacgaac ggctgctgcc ccgggaggcg gcgacgctac gcgcgctggc cgcggcgggc
     6421 tactcccctc cccgcccctt cccataactg gggctccgcc ccagcccccg ccctggggct
     6481 gcgccccaga ccccgaaacc ggtgctgcgc tccagccccc gtaccggtgc tgcgctccag
     6541 accccgtacc ggtgctgcgc tccagccccc aggccggtgc tgcgctccag cccccgtacc
     6601 ggggctgcgc cttagccccc gggccggggc tccgccccag acccaggccc ccgggcgggg
     6661 ctgtgcctca gaccccagcc cggggctccg ctccagacct cagaccccgg gtcggggctg
     6721 cgccccaggt ctcggggtcc aggggcggag cccctggtat cgggaagggg cggggagggg
     6781 aaagcgtcga tatgcggctc catcgcgggg tctgccggac atcccgtaga cgtacctgcg
     6841 cctaaggggt cggcgtcgag cgatgggcgg gtgattactg gggggtgccg gaggcgcttc
     6901 cgtaggaggt gcctccttcg atggtgccgc cgaactcgtc ccggagccgc tccatgttct
     6961 tccggggcga ggacgacagc acccagcggt tgcccaccag atagaccccg ccgtacatct
     7021 gcgccgtctc cagccagtcg cgctgcccct tggcggtgac gaagtcgagg aagacgaact
     7081 tgcccttggc gctcttgcag acggcctggc ggtaatcgtc caccttggtg gtgaactccg
     7141 gctcgcaacc ggcggccgag gcgagctgtt ccacccgcgc ggccttgggc gcggggctcg
     7201 cgctcggccg ggcggcgttg cccccggtct ccgccttggc gtccgcctta ccgcccccgc
     7261 ccccgccccc accgcacccg gcgagggcca gcagcgcccc caccgccgcc gcgaccgcca
     7321 ccgcgcgcgg ggcgcggcgg cgcggggcgc ggtcggcgat ccggaccggg tgcggaacgt
     7381 cggcgtcggc ggcgtcgtac atggaaggcc ctctcgtcgc ggatccggga tgcgggcggc
     7441 tgagtccacc gcacccacgg atacggatgg gtcgagccga tcgttcaccg tccacgcgcc
     7501 gccccgggcc cggccgggtg cgcaggggag tgtcccaacc cttgacagcc aggtcggatg
     7561 ctggattact ggaccgcgcc cgaccggtaa ccgaatgttc ggtcggcaat cggaggtggt
     7621 ggacggccca tggcgggatc cccgagcgcg gctccgctcg acgacgccga gcggatgagc
     7681 cctgaggagc tgagggcgct tcagctcacc cgcctgcgcg ccaccctgcg ccatgcgtac
     7741 gaccatgtcg agctgtaccg caagaagttc cgcgaggccg gggtcggccc cgaggactgc
     7801 cgcaccctgg aggacctggc ccggttcccc ttcaccacca aggccgatct gcgtgacagc
     7861 tatcccttcg ggatgttcgc cgtcgagcag agcgaggtgc gccggatcca cgcctccagc
     7921 ggtaccaccg gccgtcccac cctcgtcggc tacaccgagc acgacctggc ggtatgggcg
     7981 gaggtgatcg cccgctcgat ccgcgccgcc ggcggtcggc ccggccacag ggtgcatgtc
     8041 tcttacggct acggcctgtt caccggcggc ctcggcgccc actacggcgc cgagcggctc
     8101 ggctgtacgg tcatccccgc ctccggggga atgaccgccc ggcaggtgcg gctcatccag
     8161 gacctcaggc ccgagatcat catgatcacg ccctcgtacc tgctgacgct cctcgacgag
     8221 ttcgagcgac agggcgtcga tccccgctcc acttccctcc aggtcggcat attcggcgcc
     8281 gaaccctgga cagaggagat gcgccgggag atcgaggagc gcatgggcat ccacgcggtg
     8341 gacatctacg ggctctcgga ggtgatcggc ccgggggtgg cccaggagtg cgtggagacc
     8401 aaggacgggc tgcacgtctg ggaggaccac ttctacccgg agatcgtgga cccgctgacc
     8461 gacgcggtgc tgcccggcgg cgaggagggg gagctgacct tcaccaccct caccaaggag
     8521 gcgctccccg tcatccgcta ccgcacccgg gatctgaccc ggctgctgcc cggcaccgcc
     8581 cgccccgcct tccggcgcat ccagaagatc accggccgtt gtgacgacat gctcatcgtg
     8641 cgcggggtca atgtcttccc cagccagatc gaggagatcg tgctgcgggt cccggcggtg
     8701 gcgccgcact tccaggtcga gctgacccgg cgcggccgga tggaccatat ggcggtacgg
     8761 atcgaggcgc gcccaggggc cgagcccgag cagcgcgcgg ccgccgcggc ggcgatcgtc
     8821 cggggagtca aggacggtgt ggggctcacg gtcgaggtgg agatcgtgga cccggagacg
     8881 ctggagcgct ccgtgggcaa gatccgccgg gtcaaggacc tgcggacgga gggctgaggg
     8941 ccttgtgtgt gggggtgtcg agcgccgtcc cgggacctcg gacggagggc tgagggctga
     9001 gggtcgagcg gcctgcgtac gggcagccga atcccacccc gggacctgtg gacggaaaaa
     9061 agctgaaccg gcgcgcgtac gagttgctga gcaccgcccc gggcgccgtc cgtggagaca
     9121 ggacggcgcg cccggaggcg attggttcag cgtgccagtt cgagggcggc cacctcgtga
     9181 tggagcggca accgcgcccc gcagacggtc gcctgggcct gcgccgcgac cctgcgccgg
     9241 tcgtcgacgg cctgcatcgg ccgcagtggc cggtgcaggt cgacccgcac cgtgagcccg
     9301 tcggcgcgga gcacccggcg cagcgaggcg ccgaagtcgt cctcgccgac gaaggcggcc
     9361 accgtggtcg gggcgccgtg ctggacgtag tccagggtca ccgggcgcac cggcgccccc
     9421 gcgtccagcg cggcctggaa ggtggcccgc cggaagctgc cgcccgtgcc cgcacaccag
     9481 gtgatgccct gcggaaaagc catcaccgac tggccgtcgc gcagcctggc cgccatctcc
     9541 cgtacggttt cggggagttc gcgcagtgag ccgcggtcga tgtacatcgt gcccgcgcgg
     9601 gtgaccatcg ggccgatcag cggccagccc gcgatctccc gcttggccag catgaccacg
     9661 ggctctatcg cgagcagcgc gatgacgtcg agccaggaga tgtggttcgc cacgaccagc
     9721 gtgccggtgc cgcccggggc gctgagcccg gtgtcgccgg tcacctccag ccggatcccg
     9781 agggagccca gcagcgcggc cgcgcgcgca cgcagcctct cgggctcggc gatcttctgc
     9841 cccgacagca gcgcgcccat caccgaccgg ccgaagaccg cggcccgccg ggcggcccgg
     9901 gtggccggga cgcggcccgc ggcgtgggcg acacagccgg tggtgcacgg ggaccaggcc
     9961 gcccaggcgt gcgcgggcag gctcatcgcg gccgggacgg tcactgcgat tcacccagga
    10021 agtagcggcg gtagcggtcg ttcatccgct cggtgtccaa cagcacgaag aagtcggcgt
    10081 cgttgaaggc ccggtcgtgc tgcggggatc cgcacatcca ggcaccgacc cgcagatagc
    10141 cacggagcag cggcggcagt tcggcgtagc tgggctcgcc gtccagcggc cgcgagggta
    10201 tccaggggtc gtgcgggtgg acccgcatct cgggcggggc ggcgtgcttg gtggtgccga
    10261 gcagccaggc gcccgcggcg gcctgctcgc cgtcggccag cggaaccgag gcgcagcccg
    10321 cgagatagcg gtgtccggac agcagaacat agcgggccag accggcccac atgaggttga
    10381 tgacggcgcc cgagcggtgg tcggcgtgta cacaggcgcg gccggcctcg accatggacg
    10441 agcggacctc ggccggaagc ccgcgcagat cgaactcggt gtccgagtag agccgctcac
    10501 tgcgccccgg gggaagcagc cggtaggtgc cgaccacggt gcccgtcgcc gtgtccgtga
    10561 cgatcaggtg gtccatgacc tcgtcgaact catccacatc gcggccgtcg accgccgcgt
    10621 ccagccgacc gcccctctct tcgccgaaca cctggtagcg cagccgctgc gccgcgtgga
    10681 tctgctccgg ggtgtgggcg atcgaggtga cgtacgagct ggtggccgcc gttgtcgagg
    10741 acgtcgtgag cacggacatt cctgttctcc gttcggggga agtcaggagc ggtggggact
    10801 ggggagggtg cggaagagcg gaagaaacgc ctcactcgcc gtgcggtgcg ctgaggcggc
    10861 gcgtaccgcg ggcatccacc agtcttgccg tggcgacgta cgtcacagtg tccgtgcggt
    10921 gattcgacga ctgccagtcg atgacgaaag catgccgctg gtagggcgtg gttacggagc
    10981 ggtagcggcg gtgtcgccgc tgtcatcggc ggcgtcggtg gcgccggtgg cggaccggag
    11041 cgggaagccc agcgcgtggg ccgcggtggc aggagtgggc tgggaccagt agtcgctcac
    11101 ggcctgggcc gtggacaggg agcgagtttc ggccagatcc agatagagga cgccgccgag
    11161 atggtctgtc tcgtgctgga cgatccgcgc gggccagccg gtgaactcct cgtccagctc
    11221 ccgccccgtc tcgtcctgtc cgcgcagccg gatccggtcc ggacggctga ccacggcggc
    11281 ccatccgggg atgctcagac acccctcgaa gaacgctgcc cggccgtcgc cgatcggttc
    11341 gtagacggga ttgaccagca ctcggtacgg ctgcggcacc cggccgcgcg cctcccgcac
    11401 ctcggccgtc acctccgccg ggtcctcgat caccgcgagc cgcagcggca caccgatctg
    11461 cggggcggcg agccccactc cgggcgcgga gtgcatcgtc tcccgcatcg ccgccagcag
    11521 ccggtccagc tggtccgggg ccagttggcc gtcgtagggc agggccggtc gccggagcac
    11581 ggggacgccc gcggagacga tcggcagcgg gcggatcccg gagagcagtt catcgacgag
    11641 ctcggagagg ctggtggtcg gcatggggac agactggcaa gacgatcacg ccacggcaat
    11701 tcgcggaggt gggagcggaa gtcggctgcg gttcggtggc gccccgaagg ggcgcggggc
    11761 ggtgtcggtg tgcggctccg cggtgtcggt gtcggtgtgc ggctccgccg cgcgggcgcg
    11821 accggccacg agggtgccat ggacgatcga cggcacgtca cggcacttcc agcggggtgc
    11881 tcagccccgc tcggcctggg agccccgctc ggtccgcgag tcccgttcgg tccgcgagtc
    11941 ccgttcggtc cgcgagtccc gttcggtccg cgagccccgt tcggtccgcg agccccgttc
    12001 ggttcgtgtg tcccgctcgg cccgtgcgtc ctgttctgcc cgtgcctccg gcagcagccc
    12061 gcggatgtcg ttcggcagta cgccgtagag cttctccatc agctccgggt ccacggccga
    12121 gtccagcact tcgaagaacg cggccgcctc gcgcagcgcc tccccctcgg tgtgctcggt
    12181 gcgccaggcg atccggcccg cgaaaccggt gaggccgaac cgctcgccgt ggtcccgccg
    12241 gtacgtccgc tcctcgggcg tgccctcgtg cgcggccgcg acccggcgca ggggctcgcc
    12301 cgcctcatgc ggcaactggg ccgccatgtg gtccgccagc ccgcccggga tgcgctcggc
    12361 gagggtttcg agcgtcgccc gtatcgcccg ctcggccgac tgccggtcgg gcagctgggc
    12421 ccgggcctgg accaggcccg tcagttcttc gtagcgcatg gtcggcgctc ctcgtggtcg
    12481 gatgcccggc tgctgtgccg agtgccctgt gccgagtgcc ccgtgccgtt cgcccgaacc
    12541 gtggcgcgcg ccgggaccgg gtccaggcgg gtgccgtccg ctcagcggat cggtggcggt
    12601 cggccgcggt tcgaaaggcg ccctgagggg gctcgggcga agtgcgccgg ggtgccgtcg
    12661 gccgctcgcg gccgcgccca cgccgcggag ccgcacatcg acacggtcgg ctcggcgaag
    12721 ccctcagccc tcgcgcgcgg cgagcagcgg gcccagcacc cgggtgccgg gtccggtgcg
    12781 cccgccgggg cccgggagct gggtggtgtt gccgaccgtc aggggctccc cgcagtgggc
    12841 gcacgcggag accggggtgg tgtcccgtcc gcagccgagg tggcggatcc gggccggggg
    12901 cccggcggag ccggcgtacc agcggtcgcc ccactccatc agcgcgagca gcaccggata
    12961 gagctcctcg cccttcgcgg tggcgcgata gtgctcacgc ggcggccgct cctggtatcg
    13021 ctcccggatc agcaccccct cctcgaccag ccgggccagc cgggcggcca ggaccttgcg
    13081 cgatatgccc aggtcatggg cgagatcgtc gaagcgggtg accccggtga gcacatcccg
    13141 catgatcagc gccgtccagg cgtcgccgaa cagatcggtg gcacgggcga tcgaacaggc
    13201 cacatcggcg agcggagtac gggtcatgac acgaccctac cggagttccc cgagggaact
    13261 aaccgatgtt aggtttcctc ggggaacccg aggcgagccc gtccggacca ccccgggcac
    13321 accggtgaat ccgcgaatcc gcgcacccgt gagggagaag acgatggccg ccccgcacat
    13381 cgaactggac tcacggtcgc ccgaggccgt cgacggccgt cagatcaggg ccgtcacctt
    13441 ccaggacagc cgcctggagt acgtccgcgg cacgctcggg aacgtagacc tccccgcgcc
    13501 cggaagccgt gccgtggtcg tgggcagcgg ccgcgggctg ctggcccggg ggctggccgg
    13561 tctcggcttc gaggtcgtgg ccgtcgatcc ctccgccgcc gcgaccgaga tggcgcgcga
    13621 ggcggacggg ggcgagcacc ccggggtcga ctaccgcacc gcgcccgccg agcgactcgg
    13681 cctcgccgac ggcgcgttcg acctcgccta ctacgcggac accttcgaga tcacctcgga
    13741 gctcgacctg gtgctcgcgg aggccgcccg ggtgctgcgg cccggcgggg tgctgatcta
    13801 cgacaccgtc aaccgcacct ttctctcccg gctgatctac ctcggcgcct tccagcgcgt
    13861 cccgatgacc cggatcatgc cggccggccg ctacaccgcg gcccggctgc gcacccccgc
    13921 cgagctgacc gccgccctgg agcggcaggg gctgcgcaac gaggacatct gcgacttcaa
    13981 gcccaaggat ccgcgcagcc tggtcaaggc caccatggcg cgccgccggg ggcggatcag
    14041 cgatgagcag atcccgccga tcgtcgactt cgtcctggcc ccggacgccc ggccgctggt
    14101 cacctatctc ggctacgccc gcaagggctg agcggcgagc ccggcacccg cccggcgagc
    14161 ccggcacccc gccccggcgg cccggggcgc acactgggcg ctgtgacgca caagcacagc
    14221 gccggactcc tgctgtaccg cggtgcggtc ccggccggtc ccgccgtcga ggtgctgctc
    14281 gcccatatgg gaggcccgtt ctgggccagg aaggacgagg gcgcctggac ggtgcccaag
    14341 ggggagtacg aggaggacga gaccccggag gccgccgcgc gccgggagtt ccaggaggag
    14401 ctgggggtgc ccccgcccgg cggtgagccg attccgctcg gctccgtgac acagaccggg
    14461 ggcaaggtgg tcatcgtctg ggcgctcagg ggcgagctgg atccggcgga gatcactccc
    14521 ggtacgttcg ccatggagtg gccgaagggc tcgggccgga tgcgggagtt ccccgagatc
    14581 gaccgggtcg cctggttcgg tctggacgag gcccgccgga agatcgtgac gaggcagcgg
    14641 atcttcctgg accggctcga agagctgctc caggaggcgg cgggcgcggg ccccggcggc
    14701 gcatgaccgt ggcccgcgcc cgccgccgtc agctcacccc gtacctcccg ttccgcagcg
    14761 caccgtggcc gccgcccagt cggcgtggtc atgcgcgttg ccgtcgccgc cgtcggtgac
    14821 ccgcagccgc agcttccgcg cgccctcgat gcgcgcctcc accggtacgg ccgcgtccga
    14881 gccgtgtgcc gtttcgcccc ggtacacccg ctcgccgtcg gcgtagacct cgaagacgac
    14941 gctgccgtcg gcgcccacct cgtcgtccag gcccgtggtc gcggtgaacg tcgagcagtt
    15001 gccgcccagg aagacctcga ccgtcgagtc ggcgtgtgtg ccgaggcccc gctcgtaggt
    15061 cgtgccgtcg atggtgagcg tcttgccgtc gcccggggcg tcctcgccgt tgctgcggtc
    15121 gcgttcgacc gggccccagc cgttgtccgc ggtgaggaag tcgagcgtgg acacggacac
    15181 ttcaccgcgt ggcgggggcg gtgtgccgtc gatctcggcc gacgccgagc cgctcacccg
    15241 cgcgccaccg gtcgaggtcg cgcggacctc ggccacggcg ggccgcgggc cgttcggggt
    15301 gccctcacca cgggtgagct cggtccgtac ggtggcggtc cggcccgccg cgagatcgcc
    15361 caggtcgagg gtgccgggcg tggcggtcca cccctcgggc agaccgaagg cggcggtgac
    15421 ctcgcgcagc ggggtgtcgc aggggttggt gacggtcagc tcggccgagg tggagccgcc
    15481 cgccggcacc gtggtgtcgg cgggggtgag tttcgcctcg ggggcgcaga ccaccgggcc
    15541 gtgcggggtg ccggtgcggg cctcggcgcc ggtcagcggg cgcagccgca gggtgtaggc
    15601 gtactccttg gcgggtcgca cctgatactc gggcagcacg gtgtggaacg tctcgctcac
    15661 cccgctcacc gcgtggtcca ggtgcagggt gttgccttcc gggtcctcgc ggagggcgaa
    15721 cgggtaggcg gcgcggtcga tcccggtgta cggggtcacg cccgccgaga agtccccgga
    15781 caccaggagc ccgctactcc cgtcggacag cgaggcccag cggacgtcct cgtggttccc
    15841 gtagtcctgc ggcttggtgt atccggcgaa ctgctcgtcc acgtcggtgg agtacacccc
    15901 caccggggca ccgtcctcgc ggtcgttgta gttctcctgc ggcccgcgcc cgtaccaggt
    15961 gaaccggtcg tagcggtcgg gcacccggag cgagagcccg aggcggggca gatagggcac
    16021 cgcgcgcgcc gcgccccggg cctctacgcg gtggtcgagg cgcagctccc cggtgccgct
    16081 gacggtgtac cgcagggtct gggcgaacga ggagtcccgc acaccgggcg cggcggccga
    16141 tgaggggacg gtcacgacga cctcgccgcc ccgctccgcc accgtgacct tgcccggttc
    16201 ggtgcgcagc cgatccaggc ccgcatcgcg ccagggcccc tcctcggagc cgatctcatt
    16261 gctgaccggg gcgcgccagg cgtccagctc gggaccggag ccgagcagct cccggccatc
    16321 ggccttcatg gagaccagtt cgccactggt ccggtcgaag ccgtacgaga agccctcgcc
    16381 ggtgacggtg aggcggccgc cggacgtggt ggcccgcacc ttccccggtg cccgcgccgg
    16441 caccgtgccc gcgagctgcc ggccgccgat gtcgaactgc tccaccgcca cccgttcccc
    16501 cgccttcgcc caggccgtgt cggacgccca caccgcctcc acggtcagct gccggtcggc
    16561 gtcccggggg ttggcgggcg gcttcggcag ctggagggtg gtgtgctgac cggggcccag
    16621 gtcgagcgtg cggctgcccc gggtcagggt gcgggcgccc tcggtgacgc gccagcgcag
    16681 ccggaggtcg tcggtgccgg tgaaggaccg ctcgttgcgc acctcgaccc ggcccgcgcg
    16741 ggcctcgtcg cccgagatcc ggatcggggc gtggacggcc gccatggcgc gggcctcggg
    16801 ctggacggtg cggtcggagg agaccacgcc gtcgaccccg ccggtcccgg cgccatagga
    16861 caggaactcc cccttgcggt cgagccggtc gaagtcgagc gcgagcaccg cgtccgccct
    16921 ggggtcggcg gcgagctgat cggggctcag cgccttgtgg tagacgcgga cctggtcgac
    16981 ggtgccgtgt gccatccggg tgccgatgtt ctcctgctcc gtctccgggt tgcggccgat
    17041 gttcaccggc tgcgcggagt ggtccacgct gccggtccag tcggccgacg cggtctgttt
    17101 cccgtcgatg agcagccgga gcgtacggcc gtcgaaggtg cccgagacgc gatgccacga
    17161 gccgtaccag ccgtcgggga catcggccgc gacggtgtgc cagtccccgt ccccgtggac
    17221 gaagaactcc agggtgtcct tgtcgcgcat cttcagtgca tagctgtggt cgcccttggc
    17281 gatcacggtg aaggagccgg tccagtcggc cggtttcacc caggcgtcca gggtgagcgc
    17341 gtccgacacc gcgtccagct tcggatcgcg gtagacctcc acgaagtcgt caagaccgga
    17401 cagctccagc gccctgcccc ggtggccggc caccagaccg ggctttcccg agacatagga
    17461 caggatgtcg ttgccggagg tgtcgggggt ggtgagcagc ggctgggtga tgttctgctc
    17521 cgcccagtcc cagatgtagc cgccctggac ctgggggtac ttccgtacga cgtcccagaa
    17581 ctcccggaag ttgccgagcg agttgcccat ggcgtgggcg tactcgccca tgatgatcgg
    17641 cttggtggtg gtcttggcct tgtccgcgag gctcgcgggg gaggggtagc gcgggcccca
    17701 gacatcggcg aacggggcgt cgccgtccgg gctgttgggc tggtggtaga ccggccgggc
    17761 cgggtcctcg cggtccagga agtcggccat cgcgtagtgg gccttgccca gccccgcctc
    17821 gttgccggtg tcccacatca gcacgctggg atggttcttg tcccgttcgt acatcgccat
    17881 gagccggtcc aggaacgccg cctgccactc gggccgttcg gccaggcagt tgtccgggca
    17941 ggcgtcgtgg tgatgggtct cgatgtccac ctcgtcgtcg atccacagac cgcgtgcgtc
    18001 ggccagttcg tagaggtacg gatccgaggg gtagtgcgag gtgcgcacgg agttgatgtt
    18061 gagccgcttc atcagggcga cgtccgaggc ggtgcgctca cgggtggcgt ggcggccggt
    18121 gtcggggtcg gtctcggatc ggttgacccc cttgacgagg atgcgctcgc cgttgacgag
    18181 gagctgtctg tccttgatct cgatctcgcg gaagcccacc ggctgggccg tggtgtgggt
    18241 gaccgtgccg tcggcgtcgg tgagccggac gacgagggtg tagaggtggg gcgtctcatc
    18301 ggtccatttg gccgggttgg ccacgtgggc ggtgagaacg gtggacccgc tgtcgccgtc
    18361 caccgtcccc gacatcgttg tcaccttgtg tccgcgctgg tcgtacaggg ttccgaggac
    18421 cttgcgcccg tccggggcgc ggtccggtgt cttcggcccc ttcgcggcga tgtcgacctc
    18481 ggcggtcagc cgggcgtcgc ggtaccgggc gtcgagatcg gtcttgaccg tgatgtcccg
    18541 cagataggtg gccggggtgg agtacagccc gaccgagcgg aagatgcccg agaaccgcca
    18601 ctggtcgtag tcctccagat gcgaccccga accccagcgg tggacctgca ccgcgacggt
    18661 gttgcgcccc gggtgcagcc ggtcgctgat gtcgaactcg gcgggggtgt agccgccctg
    18721 gtcatagccc gcgtaggagc cgttcaccca caccagatag ccgctggtga cgccctcgaa
    18781 gcggaggaat gtccgccgct tctcccagct ctccggcagc tcgaaggtct tgacgtaggc
    18841 gccggtcgga ttgacgtcgt gcgggacctt cgggggatcg tccggataca tctcggtggg
    18901 gatgttccgg aacatcggat ggtcgagacc gtccgtctgc caggtgtgcg gcacgctcac
    18961 gctgcgccag ccgccctcgg aggtgtcata gccctcggtg aagaagtcct tgggcacctc
    19021 ctcggggcgg tcggacatat ggagcttcca ggtgccgtcg agcgaggcgg tccagcggct
    19081 cctggagccg ccgccgaggg cttcggcggt gtcggcgtac ggggtcagcc gggcgtgggc
    19141 gggctcctgc ccgaccgagg tgacctcggg gtcctccagc agctggtaca catccgcggg
    19201 cggatccttg ggatccgccg ctgccgccgg ggcggtgggg acggtcagcg cgagggccag
    19261 ggcgagggcg gcgagccggg ggaagcgcga aaggcgggag aaaaggcggg agaagcggag
    19321 ggggtggcga aaacggggga gaggtagacg acggcggagg cgtcggagca tctggtggtc
    19381 cacccttcta tggggtgttg aaacgttcca gagcgaacag gtccgcacat tagccaacgc
    19441 gccccctcgc gccaagatgt cgcgcggcac tcaccctcgg cccggcgcgg ctaccgtgaa
    19501 gagagtgagg gccgtgcgga atgccgtgcc gagcgcgcgg tgaggccgac ggaggcaccg
    19561 tgggtatgga gagcgccagg agtacggagt atccgccccg gctgcggctg gatgagctgc
    19621 tggaagagtt gcaggtgcgg atcgacgggg tgcgcggcac ccaggaccgg gtccacagtc
    19681 tgctggaagc ggtcctcacc gtcggccggg agctcgatct ctcgcatgtg ctgcgcagga
    19741 tcgtcgaggc cgccgtggtg ctggtggacg ccgaatacgg ggcgctgggg gtgatcggtg
    19801 aggatcagcg gctggccgaa ttcctcccgg tgggcgtcga caaggagcgg gccgaggcga
    19861 tcgggcggct gccggcgggc cacggtctcc tgggcgagct gatccgccac cccaagccgc
    19921 tgcggctggg cgagctctcg gaacatcccg cctcctacgg ctttccgccg aaccatccgc
    19981 cgatgcgctc cttcctcggg gtgccgatcc gggtgcgcga cgaggtcttc ggcaacctct
    20041 acctcaccga gaagcgcggt ggtgaggagt tcgacggcga ggacgagacg gtgctgtcca
    20101 ccctggccgt ggccgccggg gtggccatcg agaacgcccg gctgtacgag gaggcccggc
    20161 accgccagcg ctggctggag gcgaacgccg aggtcaccca cagcctgctg tccggagtgg
    20221 acgagaccag ggtcctggag ctgatcgtcg agcacgcgcg gcggatcctc tccgcggatc
    20281 tcggagtgct gatgctgccg gtggccgaca ccgacgaact ccaggtcgcg ctggcgtcgg
    20341 ggaccgacgc cgaggcccac cgcggtcttg tactgccccg ccggggcacc ttcggcgggg
    20401 cggccttcac cgcctccgag ccggtcacca gcaccgatgt ccagaacgac ccgcggatca
    20461 cggtcggccc gcagcgctgg gaggggctcg gcccggccgt cgcggtgccg atgcggacgc
    20521 acgatggagt gggcggggtg ctctcgctgg cccgtgtggc ggacagccac gccttcaccc
    20581 aggtcgagac cgacaccctg ctggggttcg ccggtcaggc ggcgatcgcg atgaaactgg
    20641 ccgagcggcg gcgcgacgcc gaacagctcg ccctgctcga ggaccgcgat cggatcgccc
    20701 gcgatctgca cgacctggcc atccagcggc tcttcgccgc cgggatgacg ctccagtcc
//
