LOCUS       NZ_CP019458            10936 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces autolyticus strain CGMCC0516 chromosome, complete
            genome.
ACCESSION   NZ_CP019458
VERSION     NZ_CP019458.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces autolyticus
  ORGANISM  Streptomyces autolyticus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10029028)
  AUTHORS   Yin,M., Jiang,M., Ren,Z., Dong,Y. and Lu,T.
  TITLE     The complete genome sequence of Streptomyces autolyticus CGMCC 0516,
            the producer of geldanamycin, autolytimycin, reblastatin and
            elaiophylin
  JOURNAL   J Biotechnol 252, 27-31 (2017)
   PUBMED   28472671
REFERENCE   2  (bases 1 to 10029028)
  AUTHORS   Yin,M., Jiang,M. and Lu,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2017) Yunnan University, Yunnan University, 2#
            Cuihu Beilu, Kunming, Yunnan 650091, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 279x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:05:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,227
            CDSs (total)                      :: 8,141
            Genes (coding)                    :: 7,920
            CDSs (with protein)               :: 7,920
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 74 of 221
            Pseudo Genes (incomplete)         :: 182 of 221
            Pseudo Genes (internal stop)      :: 9 of 221
            Pseudo Genes (multiple problems)  :: 42 of 221
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:31:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9239752
            Orig. end                         :: 9250688
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019458.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10936
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="41"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10936
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10936
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5936](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5936
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(229..245)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 19.95"
                     /tool="antismash"
     gene            708..1727
                     /locus_tag="BV401_RS38180"
                     /old_locus_tag="BV401_38260"
     CDS             708..1727
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056762.1"
                     /locus_tag="BV401_RS38180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38260"
                     /product="aldo/keto reductase"
                     /protein_id="WP_079259853.1"
                     /transl_table=11
                     /translation="MNAHAQTHTTAHASPGGVALPGTPVPLSRLVLGTMTFGDTVDRAG
                     AAAMLDTALDAGVTGVDTANAYAGGTTETILAELLPGRRDRIVLATKAGMPHPDAGDHS
                     PLSARGMRAALEGSLRRLGTDHVDLFYLHQPDRSTPLEETLGTVAEFLAEGKIRALGVS
                     NYAAWQIAEITHTAERVGAPRPVVAQQLYNLLARRIEEEYREYAATSGLRTMVYNPLGG
                     GLLTGRHTFQERPESGRFGDSRVAAMYRERYWDAGLFEAVRDLGRIADEASIPLTDLSL
                     RWLLFRDGVDALLLGGSRIEHLRSNLDAATAGPLPDDVVAACDEVGARLRGPMPAYNR"
     gene            2306..3511
                     /locus_tag="BV401_RS38190"
                     /old_locus_tag="BV401_38265"
     CDS             2306..3511
                     /EC_number="3.2.1.18"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712848.1"
                     /locus_tag="BV401_RS38190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38265"
                     /product="exo-alpha-sialidase"
                     /protein_id="WP_079259855.1"
                     /transl_table=11
                     /translation="MTADHDTALPPTDGRLRVRPGDPACQEAFLPAPAVQNHAANLTVL
                     PGGDLGCVWFGGTQEGVPDISVWFSRLAPGSDTWTEPVRLSGDATRSEQNPLLFPTPSG
                     ELWLLYTAQRAGDQDTAEVRLRVSADAGATWDAPRTLFPATEAGGVFIRQPVAVLDSGR
                     WLLPVFHCVATPGVKWAGDHDTSAVMISDDQGRTWRERPVPGSTGCVHMNVHQLPDTTL
                     LALFRSRWADAVYRSHSTDGGETWSEPVRTELPNNNSSVQYVPLADGRLALVYNHSSRA
                     DATARRVSLYDEIDDAGHTARAPAPATARDASAPGAFWGAPRAPMTLALSSDNGLTWPV
                     RRDLDTGDGHCLTNNSRDRLNRELSYPTIRQGPDGTLHIAYTYHRQAIKYVRVAPDWAA
                     DG"
     gene            3504..4187
                     /locus_tag="BV401_RS38195"
                     /old_locus_tag="BV401_38270"
     CDS             3504..4187
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (7-163). E-value: 5.8e-11. Score:
                     34.6. Matches aSDomain:
                     nrpspksdomains_BV401_RS38195_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056764.1"
                     /locus_tag="BV401_RS38195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38270"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_069867281.1"
                     /transl_table=11
                     /translation="MAEPRHAVVTGVSSGIGAAIATRLLAEGWRITGLSRTAPAHTVAG
                     SHWIPADLSRPETLPELLAPVAGVDAIVHAAGVQRSARLGELEPEDGALMWRIHVQAAG
                     VLVDTLVDRIADGGRVVLVGSRTMTGVPGKSQYAATKAALPALARSWAAELAPRAVTVN
                     VVAPGPTDTPMLRDPGRSRTPPVLPPLGRLVRPEEVAGLTSFLLGPEGGAVTGQTLVMC
                     AGASL"
     aSDomain        3525..3992
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BV401_RS38195_PKS_KR.1"
                     /evalue="5.80E-11"
                     /label="BV401_RS38195_PKS_KR.1"
                     /locus_tag="BV401_RS38195"
                     /protein_end="163"
                     /protein_start="7"
                     /score="34.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VVTGVSSGIGAAIATRLLAEGWRITGLSRTAPAHTVAGSHWIPAD
                     LSRPETLPELLAPVAGVDAIVHAAGVQRSARLGELEPEDGALMWRIHVQAAGVLVDTLV
                     DRIADGGRVVLVGSRTMTGVPGKSQYAATKAALPALARSWAAELAPRAVTVN"
     gene            complement(4251..4892)
                     /locus_tag="BV401_RS38200"
                     /old_locus_tag="BV401_38275"
     CDS             complement(4251..4892)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056765.1"
                     /locus_tag="BV401_RS38200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38275"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_069867280.1"
                     /transl_table=11
                     /translation="MQERAEQTRRSLLEAAAFLFDERGYAGTSISDITSRSGHTSGAIY
                     FHYTSKERLALAVVEEHFASWPPLIERYTALDSPPLEQLVRLSFAVARAFRDDIVVRAG
                     ARLWAERTLIEAPMPPPFVGWIDAVGQMMEKAGAEGDLAPHVDPLPAARTVVCAFFGLH
                     TVSEALDGRRLVEDRLADLWTLLLPSLQARPGDPAGLLALARADSPPAPE"
     misc_feature    4903..4919
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.49"
                     /tool="antismash"
     misc_feature    complement(4981..4990)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.18"
                     /tool="antismash"
     misc_feature    4983..4996
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.63"
                     /tool="antismash"
     misc_feature    4986..4999
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.73"
                     /tool="antismash"
     misc_feature    complement(4988..5001)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.9"
                     /tool="antismash"
     misc_feature    4989..5002
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.6"
                     /tool="antismash"
     gene            5001..5936
                     /locus_tag="BV401_RS38205"
                     /old_locus_tag="BV401_38280"
     CDS             5001..5936
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056766.1"
                     /locus_tag="BV401_RS38205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38280"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_069867279.1"
                     /sec_met_domain="AfsA (E-value: 7.1e-19, bitscore: 66.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVIVREAHGLSEPSGPAGRPLSWSRTVAREAVHRVSVAEVLLTDV
                     RTHGDNRFEAAAQWPRSHPTFPHGGDGRHHPLMIAETLRELGIYIPTRYYAVPAGAHFL
                     IRDISYRLDPETEPRAPYGASDITCRAAVTDIRTSPCGRFVTGMRLDVVLSAGGKLFAW
                     AGGTARFLDGTTYAETRAAAHGHTARRRLRAAVRPSPAQLAVPAARDVLVVRDGGVVYL
                     DPADPRHPFFFDHWSDHMPGLVLLEGARQAAALATGGTLIRPVVCRMKAIRFTESYPPA
                     VVECTSHGRTCVFRLRQAGICTAVGVLQYL"
     misc_feature    complement(6057..6077)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            6225..6902
                     /locus_tag="BV401_RS38210"
                     /old_locus_tag="BV401_38285"
     CDS             6225..6902
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056767.1"
                     /locus_tag="BV401_RS38210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38285"
                     /product="HAD family phosphatase"
                     /protein_id="WP_069867278.1"
                     /sec_met_domain="HAD_2 (E-value: 7.6e-22, bitscore: 77.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAEKFLGWTPAAIVFDCDGTLMDSERHWEEAREVVLRSHGTAPD
                     EEFARRTKGLHYTECGRMLAEFAGRPELAEEMTEQLLDAFRRLVADDPVTMPGAPQLVA
                     KAATFAPLAVASNCPRDVVEDGLAKAGLLRYFGHVLVPEGEVRPKPYPDVYLEAARLCG
                     AAPADALAVEDSHCGFLSASRAGLRVLGVGPRKPVDESPPVDVWVSTLADPDLVKWADS
                     RAA"
     gene            complement(6946..7470)
                     /locus_tag="BV401_RS38215"
                     /old_locus_tag="BV401_38290"
     CDS             complement(6946..7470)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056768.1"
                     /locus_tag="BV401_RS38215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38290"
                     /product="hypothetical protein"
                     /protein_id="WP_079259856.1"
                     /transl_table=11
                     /translation="MSLALRATAVCAGLLFLAGCGGNDQADGARHEQKKISPATKEFAE
                     PGTPAPLTKLADAIGCKATVITEADELRQGACGSGAKRFTMVTFATDKGQHDWLTTSKD
                     YGGMYLVGKRWSVTGLSMNSLEPLREKIGGSLEKGMSHGASHGGKKPEDGMEGMEGMDG
                     SASHGGHDGKK"
     gene            complement(7562..8536)
                     /locus_tag="BV401_RS38220"
                     /old_locus_tag="BV401_38295"
     CDS             complement(7562..8536)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056769.1"
                     /locus_tag="BV401_RS38220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38295"
                     /product="SCO0930 family lipoprotein"
                     /protein_id="WP_079259857.1"
                     /transl_table=11
                     /translation="MEKRRHIAFAGVSVAMVLLTAACGSSKDNTAGSANVQPAGGSQTV
                     GAGASASAGAVAGAGGGYEAGGDTGAAAGGAAERTGPAKQLAVKEDAKLGKFITDSKGW
                     TLYRFDEDTPKPAKSNCNDDCATKWPAVPADDATATTGIDSAKLGSVTRSDGTKQLTLA
                     GWPVYRFAGDDKPGDTKGQGVGGTWNALAPDGKPAGKTAATDDSLQLMVNNNAELGKII
                     VDGDGMTVYRFNKDSAWPMKTGCLGACLDTWKPVKAVDTTKVAGVDAAKVTSFKRPDGT
                     KQAAFDCWLLYTFTGDKKPGDINGQGVKGTWFAVTDQGKKAGQ"
     misc_feature    8513..8523
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 17.67"
                     /tool="antismash"
     gene            complement(8747..10261)
                     /locus_tag="BV401_RS38225"
                     /old_locus_tag="BV401_38300"
     CDS             complement(8747..10261)
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056770.1"
                     /locus_tag="BV401_RS38225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_38300"
                     /product="NADH-quinone oxidoreductase subunit NuoF family
                     protein"
                     /protein_id="WP_069867543.1"
                     /transl_table=11
                     /translation="MITTKPRLACVDPPRLLAGLDEVYRLDRVGHLTVHGTMPALRADE
                     LVALAENIDLRGRGGAGFPFAKKVQAVVESAGRREGRCAVVVNGSEGEPSCLKDTALLL
                     HTPHLVIDGAVLAAEAIGAEEVAIAVHRPDVEESLAAAIAERGPSGVPVRVSRTPDRFV
                     AGEGGAVINGISGAPAIPNGRKIRTSDSGLSGLPTLLSNTETFAQLAVAARMGALPYRS
                     VGLPTEPGTTLLTVGGKYVMETPTGVPLTYVLELCGLTAGQAVLVGGYHGKWLDPRSIN
                     AATISRESMKKYGARLGAGAVLPIPENTCPLGETARVARWLAAETAGQCGPCFIGLPAI
                     ADAIGQVERGGGQAAIDNVRAYVNSVKKRGACSHPDGTAGFVTTALEAFPEEFEAHALG
                     AGCGRPTMGVLPLPEDALPLALPPAPLNPSARDDRSQRGPMEKLLVDWSLCQGHGLCAD
                     ILPPEVLTLGIDGYPASASMEVPRQLRAQAVRAVRRCPALALRIEE"
ORIGIN
        1 cgcagcagcg agtcgatctt cttcagtacc agggtgtcca gggcatctgg agcgttctcg
       61 tccggcgagg acagccgcag cgcctcgcgg accgcctcgg ccgcctcggt ggccgggcga
      121 tagcgggagt cgaggtccag gacggtcgcc tcgccggggt ggcgggggcg tagagccctc
      181 gccaccccgc cgttcaccgc accgggcctg cctatcagca gcacccgact gcgtgttgtg
      241 cgcgaatgaa gcgccgccgc cacttccgcg gcgcctgaca ggtcatccgc gatggccaac
      301 agcctccggc tggccgccat gtccaccggt acctcctcga atgaccatga ccggcggagc
      361 gatgcgctgc tggcgacatc gtctccgtgg tcccgatgat gacatacttt gcgcgaagtg
      421 cgcgagagct attgcgtaaa gcgcgcagtc atgtcacggt gaccgtcgcg gcactttgac
      481 ccgtacggcc gtcatccggg cgccgcacgg gtcgagcgag tggagcgagt cgagcgaacc
      541 ggtcgctcgc ctcagaactg ccgagaggtc aacgctgacc cgtaccccct acgttccggc
      601 cctcccgtgt ccacgatcgc gaccgaccgg gaaccacggg aacgggaact atgggaacca
      661 caagacccac gggaaccacg ggaaccacag gaaccaggag caacgcagtg aacgcgcatg
      721 cgcagaccca caccaccgcc cacgcgtcgc ccggcggggt cgccctcccc ggaaccccgg
      781 tgccgctctc gcggctggtg ctgggcacca tgaccttcgg tgacacggtg gaccgcgcgg
      841 gcgccgccgc gatgctggac accgcgctgg acgccggggt gacgggcgtg gacaccgcca
      901 acgcctacgc gggcggcacc accgagacga tcctggccga gctgctgccc ggccgccgcg
      961 accggatcgt gctcgccacc aaggccggta tgccgcaccc ggacgccggt gaccactcac
     1021 cgctgtccgc gcgcggaatg cgcgcggccc tcgaaggcag cctgcgccgt ctgggcaccg
     1081 accatgtcga cctgttctat ctccaccagc ccgaccgctc cacaccgctg gaggagacgc
     1141 tgggtacggt cgccgagttc ctcgccgagg gcaagatccg cgcgctcggc gtctccaact
     1201 acgccgcctg gcagatcgcc gagatcaccc acaccgccga gcgggtcggc gccccgcgcc
     1261 cggtggtggc ccagcagctc tacaacctgc tggcgcggcg gatcgaggag gaataccggg
     1321 agtacgcggc caccagtgga ctgcgcacca tggtctacaa cccgctcggc ggcgggctgc
     1381 tgacggggcg tcatacgttt caggagcggc cggagtcggg ccgcttcggc gactcccggg
     1441 tggccgccat gtaccgggag cgctactggg acgctgggct cttcgaggcc gtacgcgacc
     1501 tgggccggat cgcggacgag gcgtccatcc cgctgacgga cctctcgctg cgctggctgc
     1561 tgttccgcga cggagtggac gccctcctgc tgggcggctc ccggatcgag cacctccggt
     1621 caaacctgga cgcggccacg gcggggccgc ttccggatga tgtggtggcg gcgtgtgacg
     1681 aggtgggggc gcggttgcgt gggccgatgc ccgcgtacaa ccggtaggcc cgtggttttc
     1741 cccaccccgc cccttcccga accaaggggc tccgcccctg gaccccgaac gcccctcggc
     1801 cccggctccc ggacggcagc gccccgcccc gcgtcgcgtg ggtgcgaccg cggcgcctcg
     1861 cttcgccgcc gtggccgcag cgtgttgctg tgggagggcc ctttcccgaa ccaggggttg
     1921 tgcccttggg ccccggacgc ccctcgggcc cggttcccgg gcggcggcgc cccgccccgc
     1981 ctccgtgtcg tgcggcgtgg ccgcagagtg ttgccgtggg agggaccccg atggccccgg
     2041 actccgtcgg gcggcgtctc tcctcggggc ggggctttgc ccgctgtgcc cgggacgcct
     2101 gtcaggcgcg tcctcccggg ccgggggtcc ggttggtatc ggggcccgtg gctcggccgc
     2161 gggtcggggc cgggggaagg ccacttcccg gcccggtcga tatgcggctc ccgcccccgg
     2221 acacccggag ccgcccctcg ggcgcgtcct tggggctgtc cgtacacatt tccgtatcac
     2281 cgcaaccctg aggagcccgg cgaccatgac cgccgaccat gacaccgctc tgccgccgac
     2341 cgacggacgg ctccgggtgc gccccggtga cccggcgtgc caggaggcgt ttctgcccgc
     2401 gcccgccgtg cagaaccatg ccgccaatct caccgtgctg cccggagggg atctcggctg
     2461 tgtgtggttc ggcggcaccc aggagggcgt accggacatc tccgtgtggt tctcccggct
     2521 cgccccgggc tccgatacct ggacggagcc ggtccggctc tccggcgatg ccacccgctc
     2581 ggagcagaac ccgttgctgt tccccacccc gtccggtgag ctgtggttgc tctacaccgc
     2641 gcagcgggcg ggtgaccagg acacggcgga ggtgcggctg cgggtgtccg ccgacgcggg
     2701 cgccacctgg gacgcgccgc gtacgctctt cccggccacc gaggcgggtg gggtgttcat
     2761 ccgtcagccg gtggccgttc tggactccgg gcggtggctg ctgccggtgt tccactgcgt
     2821 ggccaccccc ggggtcaaat gggccggaga ccatgacacc agcgcggtga tgatcagcga
     2881 cgaccagggg cggacctggc gtgagcggcc cgtgccgggc tcgaccggct gtgtgcacat
     2941 gaacgtccac caactgccgg acaccaccct gctggccctc ttccgcagcc gctgggcgga
     3001 cgcggtgtac cgctcgcaca gcaccgacgg cggggagacc tggagcgagc cggtccgcac
     3061 cgaactgccc aacaacaact cctccgtgca gtacgtcccg ctggccgacg gccggctcgc
     3121 gctggtctac aaccacagca gccgggcgga cgccacggcc cgccgcgtct ccctctacga
     3181 cgagatcgac gacgcggggc acaccgctcg ggcccccgcc ccggccaccg cgcgggacgc
     3241 ctcggcaccc ggcgccttct ggggcgcacc gcgcgccccg atgaccctcg cgctgtcctc
     3301 cgacaacggc ctgacctggc ccgtacggcg cgatctggac accggcgacg gccactgcct
     3361 gaccaacaac tcacgcgacc ggctcaaccg cgagctgtcc taccccacca tccggcaggg
     3421 ccccgacggc acactgcaca tcgcgtacac ctaccaccgc caggccatca aatacgtccg
     3481 tgtcgcaccg gattgggcgg ccgatggctg agccccgcca cgccgtggtc accggtgtca
     3541 gctcgggtat cggcgccgcc atcgccaccc ggctgctggc cgaggggtgg cggatcaccg
     3601 ggctgagccg tacggccccc gcgcacacgg tcgcggggtc gcactggatc ccggccgatc
     3661 tgtcccggcc ggagaccctc cccgagctgc tcgccccggt ggccggggtg gacgcgatcg
     3721 tgcacgcggc gggcgttcag cgctcggcgc ggctggggga gctcgagccc gaggacggtg
     3781 cgctgatgtg gcggatccat gtgcaggcgg cgggcgtcct ggtggacacg ctggtggacc
     3841 ggatcgcgga cggcggccgg gtggtgctgg tgggcagccg gacgatgacc ggggtcccgg
     3901 gcaagagcca gtacgccgcc accaaggcgg cgctgcccgc cctggcccgg tcctgggcgg
     3961 ccgagctcgc gccccgcgcg gtcacggtga acgtggtggc gccggggccg acggacaccc
     4021 ccatgctgcg cgaccccggc cgtagccgca ccccgccggt gctgccaccg ctgggccggc
     4081 tggtccgccc ggaggaggtg gcggggctga cgtcgttctt gctgggcccg gagggcggcg
     4141 cggtcaccgg ccagacgctg gtgatgtgcg cgggcgcctc gctctgaccg ggccggggga
     4201 gccgccgggg gcccttcact ccgacgtggc agggcgtgga cgcaggcgcc ttattccggc
     4261 gcgggcgggg agtcggcgcg ggcgagcgcc agcagccccg ccggatcccc gggccgggcc
     4321 tggagcgagg gcagcagcag ggtccacaga tcggccaggc ggtcctcgac cagccgccgc
     4381 ccgtccaggg cctcggagac ggtgtgcagc ccgaagaagg cacagaccac ggtccgggcg
     4441 gcgggcagcg gatcgacatg cggggccaga tcgccctcgg cccccgcctt ctccatcatc
     4501 tggcccacgg cgtcgatcca ccccacgaac ggcggtggca tgggcgcctc gatcagggtg
     4561 cgctcggccc acagccgggc tccggcgcgg accacgatgt catcgcggaa ggcgcgggcc
     4621 accgcgaagc tgagccgcac cagttgctcc agcggaggcg agtcgagggc ggtgtagcgc
     4681 tcgatcagcg ggggccagga ggcgaagtgc tcctccacca cggccagcgc gagcctttcc
     4741 ttgctcgtgt aatggaaata gatcgcgccg ctggtgtggc cggacctgga cgtgatgtcg
     4801 ctgatgctcg ttccggcgta tccccgttcg tcgaaaagga atgcggcggc ctccagaagg
     4861 gatcggcgtg tttgttcggc ccgttcttgc acgtgttcgc cgtccttaca ccagcacatc
     4921 ggatgtgacg gggagaactt agcggacttt cggccagtga tggtgatgag gcgagacagg
     4981 tcaaacatta ttagtatttt atggtgatcg ttcgtgaggc tcatggcctt tcggagccgt
     5041 ccggtcccgc cgggcggccg ctcagctggt cccgcacggt ggcgcgggag gcggtgcacc
     5101 gggtctcggt ggccgaggtg ctgctcaccg atgtgcgtac gcacggtgac aatcgattcg
     5161 aggccgcggc ccagtggccg cggtcccatc ccaccttccc gcacggcggg gacggccgtc
     5221 accatccgct gatgatcgcg gaaaccctgc gcgaactggg gatctacatc cccacgcggt
     5281 actacgcggt gcccgcgggg gcgcatttcc tgatccggga catctcctac cggctggacc
     5341 ccgagaccga gccccgggcg ccgtacggcg cctccgacat cacctgccgg gccgccgtca
     5401 ccgacatccg caccagcccc tgcgggcgtt tcgtcaccgg gatgcggctg gatgtcgtgc
     5461 tgtcggcggg cggcaagctc ttcgcctggg ccgggggtac cgcgcgcttc ctcgacggca
     5521 cgacctacgc ggagacccgg gccgccgccc acggacacac cgcgcgccgt cggctgcgcg
     5581 ccgcggtacg gccctcacct gcgcaactgg ccgttcccgc ggcccgggac gtcctggtgg
     5641 tgcgggacgg gggagtggtc tatctcgacc ccgccgaccc ccgccatccg ttcttcttcg
     5701 accactggag cgaccatatg ccggggctgg tgctgctgga gggcgcccgc caggcggcgg
     5761 cgctggcgac cggcgggacg ctgatccggc ccgttgtctg ccggatgaag gcgatccgct
     5821 tcaccgagtc gtatccgccg gccgtggtcg agtgcacctc ccacggcaga acgtgcgtct
     5881 tccggctgcg ccaggccggt atctgcactg cggtcggcgt gctccagtac ctctgagcga
     5941 cgaacgcacc gtgcgaagaa cacgccgtgc gacgaactct ttggcgacaa acggactgaa
     6001 cgacgaacaa aacgccgaca ctcggtgacc ccttgcttac tgcgggtcat caaactaacg
     6061 tagtgatcgt tatgtttcgg gcaggcgatt ctcgcggcct gtacgacaac agcggtcggc
     6121 gcccagcgcc cttctgagcc gccaggacat ttccactgcg cggcccccgc aggtacaggc
     6181 acaccctgcg ccgattcctc cacctcccat ggagcggcag acagatgaca gccgaaaagt
     6241 ttctcggatg gaccccagcc gcaatcgtct tcgactgcga cggcaccctg atggattccg
     6301 aacgacactg ggaggaggcg cgtgaagtgg tgctcagaag ccatggaacc gcccccgacg
     6361 aggagttcgc ccggcgcacg aaggggctcc actacaccga atgcggccgc atgctggccg
     6421 aattcgccgg gcgccccgaa ctcgccgaag aaatgaccga acaactcctg gacgcgttcc
     6481 gccgactggt ggccgatgat ccggtcacca tgccgggggc gccccaactg gtcgcgaagg
     6541 ccgcgacctt cgcgccgctg gccgtggcca gcaactgccc gcgggacgtc gtcgaggacg
     6601 ggctcgccaa ggccgggctg ctgcgctact tcggccatgt gctggtcccc gagggggagg
     6661 tgcggcccaa accgtatccc gacgtctatc tggaggccgc ccggctgtgc ggtgcggccc
     6721 ccgcggacgc cctcgccgtg gaggactccc actgcgggtt tctgtccgcc tcgcgcgccg
     6781 ggctgagagt gctcggcgtc gggccgcgca agccggtgga cgaatccccg ccggtcgacg
     6841 tatgggtctc caccctcgcc gacccggatc tggtcaagtg ggcggactcc cgggcggcct
     6901 gaggggctga cccgcgcaca ccgcctggtc ggcgggcgcc gcgcgtcact tcttcccgtc
     6961 gtggccgcca tggctcgccg agccgtccat gccctccatg ccctccattc cgtcctccgg
     7021 cttcttcccg ccgtgggagg cgccgtggga catccccttc tcgagggatc cgccgatctt
     7081 ctcgcgcagg ggctccagcg agttcatgga caggccggtg accgaccagc gcttgccgac
     7141 caggtacatc cccccgtagt ccttcgacgt cgtcagccag tcgtgctggc ccttgtcggt
     7201 ggcgaaggtg accatcgtga accgcttcgc accggacccg caggcgccct ggcgcagctc
     7261 gtccgcctcg gtgatgaccg tggccttgca gccgatggcg tcggcgagct tcgtcagtgg
     7321 cgcgggcgtg ccgggctcgg cgaactcctt ggtggcgggg gatatcttct tctgctcatg
     7381 tctggcgccg tctgcctggt cgtttccacc gcagccggcc aggaacagca aaccggcgca
     7441 gaccgcggtg gcacggagtg cgagtgacac atcaacctcc cgtagaacgt ccggcggggc
     7501 ggtccgggcc cggatggcct cggaccaccc gccgcggtgg accgtcagta tggtgtccgc
     7561 cctactggcc cgccttcttg ccctggtcgg tgaccgcgaa ccaggtgccc ttcacgccct
     7621 ggccgttgat gtcaccgggc ttcttgtccc cggtgaaggt gtacagcagc cagcagtcga
     7681 acgcggcctg cttggtgccg tcggggcgct tgaaggaggt gaccttcgcc gcgtcgaccc
     7741 cggcgacctt ggtggtgtcc acggccttga cgggcttcca ggtgtccagg caggcaccga
     7801 ggcagccggt cttcatcggc caggcgctgt ccttgttgaa ccggtagacg gtcataccgt
     7861 cgccatccac gatgatcttg ccgagctcgg cgttgttgtt gaccatcagc tggaggctgt
     7921 cgtccgtggc cgcggtcttg ccggccggct ttccgtcggg cgccagcgcg ttccaggtgc
     7981 cgcccacgcc ctggcccttg gtgtcacccg gcttgtcgtc gcccgcgaag cggtagaccg
     8041 gccagcccgc aagcgtcagc tgcttggtgc cgtcggagcg ggtgaccgag cccagcttgg
     8101 cggagtcgat accggtggtg gccgtggcgt cgtccgcggg gaccgcgggc cacttggtgg
     8161 cgcagtcgtc gttgcagttg gacttggccg gcttgggggt gtcctcgtcg aaccggtaga
     8221 gcgtccagcc cttgctgtcg gtgatgaact tgcccagctt ggcgtcctcc ttgaccgcca
     8281 gctgcttcgc cggaccggtc cgctcggccg cgccgcccgc ggccgcgccc gtgtcgccgc
     8341 ccgcctcgta cccgccgccg gcgccggcca ccgcgcccgc cgaggcggag gcgcccgcac
     8401 ccacggtctg gctgccgccc gcgggctgca cattcgcgct gcccgcggtg ttgtccttgc
     8461 tgctgccgca cgccgccgtc agcagaacca tcgctaccga caccccggcg aatgcgatgt
     8521 gacgccgctt ctccatgacc attcctctgc aatctcgatc ggtccggcgg tgattgcgcc
     8581 gacgtcatgt gcatacggag cccatgcgga ccggcgttca gcggcctgcg aatttgtcac
     8641 cggaggcaaa aaactccgga tcgctgagcg cacagcagtc ggcggccgtt tctcacggcc
     8701 gccgcggtgt cggtggtgcg gtggtgccag atggatccgc ctccggctac tcctcgatgc
     8761 gcagcgccag ggccgggcag cggcggaccg cgcggaccgc ctgcgcccgc agctgccggg
     8821 gcacttccat cgaggccgag gccggatagc cgtcgatccc gagcgtcagc acctcgggcg
     8881 gcaggatgtc cgcgcacagc ccatggccct ggcacagcga ccagtccacc aggagcttct
     8941 ccatcggccc gcgctgggag cggtcgtcgc gcgccgaggg gttcagcggg gcgggcggca
     9001 gggccagtgg cagcgcgtcc tccggcagcg gcagcacacc catcgtgggc cgtccgcacc
     9061 cggcgccgag cgcatgtgcc tcgaactcct cggggaacgc ctccagcgcg gtggtcacga
     9121 aaccggcggt gccgtcggga tggctgcacg ccccgcgctt cttcaccgag ttgacgtagg
     9181 cgcgcacatt gtcgatcgcg gcctggccgc cgccgcgctc cacctggccg atggcgtccg
     9241 cgatcgccgg cagcccgatg aagcacggcc cgcactgacc ggccgtctcg gcggccagcc
     9301 agcgcgccac ccgcgcggtc tcgccgagcg ggcaggtgtt ctcggggatg ggcagcaccg
     9361 ccccggcgcc cagccgggcg ccgtacttct tcatcgactc gcgggagatc gtggccgcgt
     9421 tgatgctcct ggggtccagc catttgccgt ggtagccgcc gaccagcacc gcctggccgg
     9481 cggtgaggcc gcacagctca aggacgtagg tcagcggcac cccggtgggc gtctccatca
     9541 cgtactttcc accgaccgtc agcagcgtgg tgcccggttc ggtgggcagc cccaccgagc
     9601 ggtacggcag ggcgcccatc cgcgcggcca ccgcgagctg ggcgaaggtc tcggtgttgg
     9661 acagcagcgt gggcagcccg ctgagcccgc tgtcactggt ccggatcttc cggccgttgg
     9721 ggatggccgg ggcgccgctg atgccgttga tgaccgcgcc gccctcaccg gcgacgaacc
     9781 ggtccggggt gcggctcacc cgcaccggga ccccgctggg gccgcgctcg gcgatcgcgg
     9841 cggccaggga ctcctcgaca tccgggcggt gcaccgcgat ggcgacctcc tcggcgccga
     9901 tcgcctcggc ggccagaacg gcgccgtcga tcaccagatg cggggtgtgc agcagcagcg
     9961 cggtgtcctt gagacaactg ggctcgccct cgctcccgtt gaccaccacg gcacagcggc
    10021 cctcgcgccg gccggccgac tccaccacgg cctggacctt cttggcgaag gggaaaccgg
    10081 cgccaccgcg cccgcgcaga tcgatgttct ccgcgagcgc cacgagctcg tccgcccgga
    10141 gggccggcat ggtgccatgg acggtcagat ggccgacgcg gtcgagccgg tacacctcgt
    10201 cgagcccggc cagcagccgg ggcggatcga cacaggcgag tctgggtttg gtcgtgatca
    10261 ccggtcccac ccgcgagagg caccgagcgg ctgactgtca cgcgggccgg gctcggccgc
    10321 acgctggcgc ggcacggtgg gatcggcccg ccgggtccgg ccctcctgca tgctgcgggc
    10381 gggcgggttc ctgacctcga tgccgtccgc ccggtcgatg tcctggcgcc gccgctggac
    10441 cagacgcagc cacagcgcga tggccacacc ggcgagcgcc gcgatgtagc cgtaggtggc
    10501 ccagatcttg gcggcgcggc ccgccttcag accgtggatc agcgcggcgc accacgcggg
    10561 gtaggcgccc acatgcagcg cccgccacca gcggtaccgc cagcgggagt tgcccgccga
    10621 ggcgaacgag ctgcgcacgg cgccggtcac cgcggcgatc acgaagagat agccggcgat
    10681 ggtgccgagc ccgatgagca ccggctgatt tgcgtccgcg aacggtagta cggcggacgc
    10741 ggcgttggtg tggctctccg cgaccttgac ccagatgtga acggccagga acagcaaccc
    10801 ggcgaccgcg gccgcccggt ggatcgcctg ggccatcagc cgctgcctgg tgtgcaggat
    10861 cagctggtcg gtcgcggcga gcccccacag caccgccgag gtgagacaga ccagtgcgag
    10921 cacacccgcg ccgaag
//
