LOCUS       NZ_CP019458            11539 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces autolyticus strain CGMCC0516 chromosome, complete
            genome.
ACCESSION   NZ_CP019458
VERSION     NZ_CP019458.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces autolyticus
  ORGANISM  Streptomyces autolyticus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10029028)
  AUTHORS   Yin,M., Jiang,M., Ren,Z., Dong,Y. and Lu,T.
  TITLE     The complete genome sequence of Streptomyces autolyticus CGMCC 0516,
            the producer of geldanamycin, autolytimycin, reblastatin and
            elaiophylin
  JOURNAL   J Biotechnol 252, 27-31 (2017)
   PUBMED   28472671
REFERENCE   2  (bases 1 to 10029028)
  AUTHORS   Yin,M., Jiang,M. and Lu,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2017) Yunnan University, Yunnan University, 2#
            Cuihu Beilu, Kunming, Yunnan 650091, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 279x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:05:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,227
            CDSs (total)                      :: 8,141
            Genes (coding)                    :: 7,920
            CDSs (with protein)               :: 7,920
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 74 of 221
            Pseudo Genes (incomplete)         :: 182 of 221
            Pseudo Genes (internal stop)      :: 9 of 221
            Pseudo Genes (multiple problems)  :: 42 of 221
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:31:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8093395
            Orig. end                         :: 8104934
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019458.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11539
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="35"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11539
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11539
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6539](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6539)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(110..1951)
                     /locus_tag="BV401_RS33360"
                     /old_locus_tag="BV401_33415"
     CDS             complement(110..1951)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (170-557). E-value: 3e-20.
                     Score: 64.8. Matches aSDomain:
                     nrpspksdomains_BV401_RS33360_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055539.1"
                     /locus_tag="BV401_RS33360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_33415"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_237287982.1"
                     /sec_met_domain="PF00561 (E-value: 1.5e-23, bitscore: 82.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYGILGPLSLSLTMSALLATPATGRPLTAPTTPTATAATP
                     STRAATAPAGAAGAPLTAAPAARSAAPAGPVAAAAPVWTTWSGSSAAAAAPPADPAAAR
                     GVALAARRAAERGVTFRRCPRAESLAAPITCGKVSVPLDYARPRGKRVSLTVSRVRASG
                     PKARRQGALIYNPGGPGASGMAFPEYAAEKSFRRLAAVYDFVGYAPRGVGRSAPLSCQR
                     PSAYAKAPTSSPPHPTAAFKRRLVARARAYVRGCVRRSGRALAHYTTLNNARDLEVLRA
                     ALGERRLTFVGASYGTYYGAVYATLFPGRVRRMVFDSVVNPAPWQIWYRDNLDQSLAFE
                     RRWRDWRRWVARHHRVYHLGATADRVLASYEKARRRLAHKPAGGVVGTAQLQSAFLRTG
                     YNDAYWDPAARALAAYLLGDSKPLIALAAPDRAQAADDENGNAVYTAVECNDAPWPREW
                     ATWDRDNTALARRAPFETWDNVAMNLPCAFWPVKPGRPLDVGNLEAVGPRTGRVALPPV
                     LLLSAERDAATPYAGAKELWHRLPGSSLVTERKAGTHGLWGGPNGCVNRHVDTYLLTGR
                     TPGRSAYCAPRPAPEPQPEPAPLPDSGKQPAALPGTP"
     aSDomain        complement(281..1441)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BV401_RS33360_Abhydrolase_1.1"
                     /evalue="3.00E-20"
                     /label="BV401_RS33360_Abhydrolase_1.1"
                     /locus_tag="BV401_RS33360"
                     /protein_end="557"
                     /protein_start="170"
                     /score="64.8"
                     /tool="antismash"
                     /translation="ALIYNPGGPGASGMAFPEYAAEKSFRRLAAVYDFVGYAPRGVGRS
                     APLSCQRPSAYAKAPTSSPPHPTAAFKRRLVARARAYVRGCVRRSGRALAHYTTLNNAR
                     DLEVLRAALGERRLTFVGASYGTYYGAVYATLFPGRVRRMVFDSVVNPAPWQIWYRDNL
                     DQSLAFERRWRDWRRWVARHHRVYHLGATADRVLASYEKARRRLAHKPAGGVVGTAQLQ
                     SAFLRTGYNDAYWDPAARALAAYLLGDSKPLIALAAPDRAQAADDENGNAVYTAVECND
                     APWPREWATWDRDNTALARRAPFETWDNVAMNLPCAFWPVKPGRPLDVGNLEAVGPRTG
                     RVALPPVLLLSAERDAATPYAGAKELWHRLPGSSLVTERKAGTHGLW"
     gene            complement(2037..2843)
                     /locus_tag="BV401_RS33365"
                     /old_locus_tag="BV401_33420"
     CDS             complement(2037..2843)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055540.1"
                     /locus_tag="BV401_RS33365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_33420"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_079259306.1"
                     /transl_table=11
                     /translation="MLWVLFLLVAWGAAIVSCTRLCLAAVAAAQPMEPDPAADGKGLSL
                     YEAAFLSGGPRRVGDLALVTMCRERRLLLAHTGWVTVVDPEGRDELERSVITAIGPEGQ
                     ALVRPVRAAFATAAPVRALGDQLVAAGLAVPASARTNVAAAVRQVRAACALVLAMAVAA
                     LLLVPPAAGRGPVASWFMLPLLLTVSCLLIARVEAHPYSRWASPAGQQLLGRIDVPPRR
                     TVAQRSGGGPDGGADDADVLTALAVHGTVALSDRALRAALKTHWGH"
     gene            complement(3985..4995)
                     /locus_tag="BV401_RS33370"
                     /old_locus_tag="BV401_33425"
     CDS             complement(3985..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714418.1"
                     /locus_tag="BV401_RS33370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_33425"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_079260711.1"
                     /transl_table=11
                     /translation="MGTIAGLVYAVVAVSSALLITGTVRARRRRVSGPVYAQAYDLLEA
                     AFLAGGPGRVADTVISTMYADGRLAIGGPGVVSVRQPVARGPVEEELLRLSANSPHGAL
                     TWLRRELMRSAAVQEIGDRLAGRGLMVRPDALRFWRRMARAHITLCAVGAFLAIMISIG
                     PVTYDGPPLIFMIAPALFIGFLIGRICAGAGKRRLTAAGKRALVAYRRESGVSGRHARA
                     RSIGRPATVSVAVVVALTGAATLTDDPQLRAQLLEAQKVPSGSGSPGYSGSSGDSGFSA
                     SDGGGSWCGGDGGGGGSSCGGSSCGGSSGGGCGGGSGCGGGGGCGGGGGGGCGGG"
     gene            complement(5001..6539)
                     /locus_tag="BV401_RS33375"
                     /old_locus_tag="BV401_33430"
     CDS             complement(5001..6539)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983658.1"
                     /locus_tag="BV401_RS33375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_33430"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_079259307.1"
                     /sec_met_domain="DUF692 (E-value: 1e-91, bitscore: 305.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRLGTGIGWRPEIADDIARLPGIDWVEVVAENICPDHVPDALRL
                     LRERGTTVVPHGVSLGLGGAERPEPGRLRALAERAEALGSPLVTEHIAFVRAGGPLTAS
                     PSIEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALISWPGEEMTEGQFLA
                     ELVERTGVRLLIDVANLRTNHVNRGEDPAKALDELPTSAIAYVHVAGGVEKDGVWHDSH
                     AHPVTQPVLDVLAELCARTAPPGVLLERDEDFPAAGELAGELAAIRAVTAAGAAGAAEA
                     GMATAGMATAGTAAGSAKPVTAAGAAEAATAAGTGEEAPAPVRASAAADEADKADKADE
                     ADKARTGADAPAADAPAPEAPLPDAPLPEVPVPDAVRERLALAQTALLSALVGDTPPPE
                     GFDRRRLRVQSAALTAKRASVIAKVAPELPEILGTGYRPAFVRYAEGRPMTGGYRRDAL
                     SFAEHLLADGRPGDSAARRRLTLWWQERSGPAPLPSHPAARFARRVRLALSGGR"
     misc_feature    6793..6813
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.73"
                     /tool="antismash"
     misc_feature    6801..6814
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.6"
                     /tool="antismash"
     misc_feature    6801..6815
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.81"
                     /tool="antismash"
     misc_feature    complement(6805..6818)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.7"
                     /tool="antismash"
     gene            7004..8983
                     /locus_tag="BV401_RS33380"
                     /old_locus_tag="BV401_33435"
     CDS             7004..8983
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055543.1"
                     /locus_tag="BV401_RS33380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_33435"
                     /product="transglycosylase domain-containing protein"
                     /protein_id="WP_079260712.1"
                     /transl_table=11
                     /translation="MRLRRWAAARIRLDYPRTGRRGVGRWLPSWRQIALFFLFCVGSLT
                     GLLSFLYLQTDIPKNLNDFATQQDNVYYWADGTEMARTGPVNRQEVPLKKVPEQVQWAI
                     LAAENASFYSDRGVSPSGLMRAVTRMVTGGETQGGSTITQQYVKNAYLNQRQTFSRKLT
                     EMFMAIKLDDRMSKRDILERYLNTSWFGRGSYGIQRASYAYYGKDVSELNASEGALLAS
                     LLKGAGTFDPTLGAKNHKRAVDRWKWVLDRMVDIGRLSKSERARYTKFPEPKAPPKPAG
                     LTGQVGYLVETAKAYVSSHTEISDADFDLGGYQIHTTFEKPKVNALAKAVEQTRDKLDP
                     ERRPEDRHVRVGAASVAPGGAIRALYGGPGYLEQGFNDANASNVPAGTSFTPFVYAAAL
                     RDGVLLQRNGARTPVTPSTLYDGNNKIAVHTPEGPYWDRGGKIVRATNDGDASYGQVSL
                     RQAIVQSINTPMIQLGMDVGLDRVRRVSIDAGLLPSSFGARVPAFSLGTATPSSIRMAS
                     AYGTFAAEGTHHAPYSVSGLTHGGQKVELRKDRARRAFSAEVAAQVDDALRGAVQSPDG
                     SARGAAMADAEVAGKGGTAQDGTSAWFVGYTKELSTAVSLSRLDPKSQELMPLEGMGGG
                     GADTSGDTYPLDIWKSYMAGRAAE"
     gene            complement(9014..10273)
                     /locus_tag="BV401_RS33385"
                     /old_locus_tag="BV401_33440"
     CDS             complement(9014..10273)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055544.1"
                     /locus_tag="BV401_RS33385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_33440"
                     /product="hypothetical protein"
                     /protein_id="WP_231981250.1"
                     /transl_table=11
                     /translation="MAAPIGALTAVAVVVGLLIALTSPGDAGGGTSDGNLPAGAASPKS
                     DRVPREPKKEDKYADWDGEVKVLGDGSTSYTGPQPHQPKPEKLKPGEKPPQFVVFSWDG
                     ALENDDHLFSRFRQVAKESDATMTFFLSGMYLLPKTKSDLYQPPQHKRGSAAISFPTDE
                     HIRETLEQLRGAWLEGNEIGSHFNGHFCGAKGGQDWSTQEWKSETEQAYSFVKRWKTNT
                     GFTDIEPLPFDYDVELVGGRAPCLEGQKTLIPAIKSLGWRYDASSPGDFQLWPSKIDGI
                     WNFPLQLVPYPESEKQVLSMDFNFLFNQSGDSTEGDPAKYAEWERLTRQGYLNGFERAY
                     NGSRAPLFIGNHFETWNGGIYMQAIEDVMRSVCRRDGVRCVSFKQLADWLDAQDPKVLE
                     KLRMLDPAESPDWSTYTKTR"
     misc_feature    10390..10405
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.05"
                     /tool="antismash"
     misc_feature    complement(10415..10430)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    complement(11095..11110)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.16"
                     /tool="antismash"
     misc_feature    complement(11191..11204)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.04"
                     /tool="antismash"
ORIGIN
        1 ggcgccggga tccttatggc ctccgggcgt gaccttacgg ccgcaggggt ggccgtaagg
       61 ccgccgcggg acggtccggc ggccgtcccg cacgggcttc ggcgccctct tacggcgtgc
      121 ccgggagcgc ggccggctgc ttgcccgaat cgggcagcgg ggccggttcc ggctgtggtt
      181 cgggcgccgg gcgcggggcg cagtaggcgg accggccggg ggtcctaccg gtcagcagat
      241 aggtgtccac atggcggttg acgcagccgt tcggaccgcc ccacaggccg tgggtgcccg
      301 ccttccgctc ggtcaccagc gaggagccgg gcagccggtg ccacagctcc ttcgcccccg
      361 cgtacggggt ggccgcgtcc cgctccgctg acagcagcag caccggcggc agcgcgaccc
      421 gccccgtccg ggggcccacg gcctccaggt tccccacgtc gagggggcgg cccggcttca
      481 ccggccagaa ggcgcacggc aggttcatcg ccacgttgtc ccaggtctcg aagggcgcgc
      541 ggcgggcgag ggcggtgttg tcgcggtccc aggtggccca ctcgcgcggc cagggcgcgt
      601 cgttgcactc cacggcggtg tagacggcgt tgccgttctc gtcgtcggcc gcctgcgccc
      661 gatcgggcgc ggcgagcgcg atcagcggct tggagtcacc gagcagatag gcggccagcg
      721 cccgggcggc cggatcccag taggcgtcgt tgtagccggt cctgaggaac gcgctctgga
      781 gctgggcggt gccgacgacc ccgcccgccg gcttgtgcgc cagccgccgc cgcgccttct
      841 cgtacgaggc gagcacccgg tccgcggtgg cgcccaggtg gtagacgcgg tggtgcctgg
      901 cgacccagcg gcgccagtcc cgccagcgcc gctcgaaggc cagtgactgg tcgaggttgt
      961 cgcggtacca gatctgccag ggggccgggt tgaccacgct gtcgaagacc atccggcgca
     1021 cccgccccgg gaagagcgtg gcgtacacgg ccccgtagta cgtgccgtac gaggcgccca
     1081 cgaaggtcag ccgccgctcg cccagcgccg cgcgcagcac ctccagatcg cgggcgttgt
     1141 tcagcgtcgt gtagtgcgcg agcgcgcggc ccgagcggcg cacacagccc cgcacatagg
     1201 cgcgcgcccg cgccaccagc cgccgtttga aggcggcggt ggggtgcggc ggggaactgg
     1261 tcggcgcctt ggcgtaggcc gagggccgct ggcaggagag cggggcggag cggcccacgc
     1321 cccgtggggc gtagccgacg aagtcgtaga cggcggccaa gcggcggaag gacttctcgg
     1381 ccgcgtactc ggggaacgcc ataccggacg cgcccggtcc gcccgggttg tagatcaggg
     1441 cgccctgacg ccgcgccttg gggccgctcg cccggacccg gctgacggtg agcgagacgc
     1501 gcttgccgcg gggacgggcg tagtcgagcg gtacggagac ctttccgcag gtgatggggg
     1561 cggccagcga ctcggcgcgg gggcagcggc ggaaggtgac accgcgctcc gccgcgcgcc
     1621 gggcggcgag cgccaccccg cgggccgcgg ccgggtcagc gggcggggcg gcggccgcgg
     1681 cggacgaccc ggaccaggtg gtccagacgg gcgcggcggc ggcgacgggg ccggcggggg
     1741 ccgctgaacg ggccgcgggg gccgccgtga gcggcgcgcc cgccgcaccg gccggggccg
     1801 tggcggccct ggtggacggg gtggcagccg tggcggtggg ggtggtgggc gcggtgagcg
     1861 ggcggccggt ggcgggcgtc gcgagcagag cgctcatggt cagcgacagg gacagcggtc
     1921 cgaggatgcc gtacagcgct gctgctctca ctggccgtcc cttcgtgcgt cgcgcgatga
     1981 aagggatatt tggcgcggcg gatgaccatg taaagcaccg ggccgcggtg tcggggtcaa
     2041 tgaccccaat gcgtcttcag cgccgcgcgc agcgcccggt cggagagggc gacggtgccg
     2101 tgcaccgcga gcgcggtgag cacatcggcg tcgtcggcac cgccgtccgg gccgccgccg
     2161 gaacgctggg cgaccgtccg ccgcggaggc acgtcgatcc ggcccagcag ctgctgaccg
     2221 gcgggcgagg cccaccgtga gtacggatgc gcctcgaccc gggcgatcag cagacagctc
     2281 accgtcagca gcagcggcag catgaaccac gaggccaccg gaccgcgccc cgccgcgggc
     2341 ggcaccagta gcagcgcggc gaccgccatc gccaggacca gcgcgcacgc cgcccgtacc
     2401 tgccgcaccg cggccgccac attcgtacgg gccgaggcgg gcaccgcgag ccccgccgcc
     2461 accagctggt cgcccagcgc ccgcaccggg gctgcggtgg cgaacgcggc ccgcaccggc
     2521 cgcacgaggg cttggccctc cggccctatc gcggtgatca cggaccgctc cagctcgtcc
     2581 cgcccctccg ggtccacgac ggtgacccag ccggtgtggg cgagcagcag ccggcgctcg
     2641 cggcacatcg tcacaagggc cagatccccc acccgccgcg ggccgccgga caaaaatgcc
     2701 gcctcgtaca ggctcaggcc cttcccgtcc gccgccggat caggctccat cggctgagcg
     2761 gcggcgaccg cggccaggca cagacgcgtg caactgacga tcgccgcgcc ccacgcgaca
     2821 agcaggaaga ggacccacaa catggccgag ttctactcga cccggccgcc accgcgccac
     2881 ggttttcacg atatgaacga ctcgcgcgaa tgcggcgcgc gcgccgcgca gacccgcgac
     2941 ggattgtgcc gcctacggcg gactgttccc ctgccccgcc ccttcccaaa actggggctc
     3001 cgccccagac cccgtatagg ggctccgccc ccagcccccg aaaccggggc tccgccccag
     3061 gccccgggtc ggggttctgc cccaggcccc gggtcggggt tctgccccag gccccgggcc
     3121 ggggcattgt ctgggctccg ggtcggggtc ctgcccgggc cccgggttgg ggtcctgcct
     3181 gggccccggg ttggggcttc gcctcaggct tcgggtcagg gcctccgccc cgggctcggg
     3241 gtcgggtttt gctcgggctt ggggtcggtg cttcgcctcg ggctcggggt cggggccctg
     3301 ccccgggctc cggggcaggg cctccgctcc tgcccgcggc atcggtatgc ggctccgccg
     3361 cgtggcgggt ctccgtcctt gaccccgggg tccgggggcg gagcccccgg tatcgggaag
     3421 gggcggggag gggaaagcat cggtatgcgg ctccgccgcg tggtctgccg gacaccccgt
     3481 aactctgctc gggaccgcgg tggggtcgtg ggcgatccgt acagctgcgg cgcgggatgc
     3541 cggtgggcgg gcggtctggg atcggcgagg acctcttgcc ggtcgccatc ggcacccggg
     3601 gctgggcggc tcgacccgcc gctgctcggt cggccgcccg cgtccggccc ctcgcccgcg
     3661 tccggcatcg cgcgcccacc cggagtcccg cttgcctccg gcgtcccgcc tgcccccggc
     3721 atcgcgcccg cgtccggcgt cccgcctgcc cccggcatcg cgcccgcgtc cggcgtcccg
     3781 cctgcccccg gcatcgcgcc cgcgtccggc gtcccgcctg cctccggcgt cccgcctgcc
     3841 tccggcgtcc cgcctgcctc cggcgtcccg cctgcctccg gcgtcccgcc tgcctccggc
     3901 gtcccgcctg cctccggcgt cccgccgggc cccggtgggc tccgccatgc ctcctggccg
     3961 cagtgcggcc acttgtgtgg cggctcagcc gccgccgcag ccgccgcccc caccgccgcc
     4021 gcagcctccg ccccctccgc agcccgaacc tccgccgcag cctccgccgc tgctccctcc
     4081 gcaggacgag ccgccgcacg aggagccccc gcctccgccg tccccgccgc accacgatcc
     4141 gccgccgtcc gaagccgaga accccgaatc gcccgaagac cccgagtagc cgggcgatcc
     4201 cgagccgctc ggtaccttct gcgcctccag caactgggcc cgcagctgcg gatcgtccgt
     4261 cagcgtggcc gccccggtca gcgcgacgac gaccgccacg gagaccgtcg ccggacggcc
     4321 gatggaccgg gcgcgggcat ggcgtccgct gacccccgac tccctccggt acgccaccag
     4381 cgcccgcttc ccggcggcgg tcagcctgcg cttgcccgca ccggcgcaga ttctgccgat
     4441 gaggaagccg atgaagagcg cgggggcgat catgaagatc agcggaggcc cgtcgtaggt
     4501 gacgggtccg atgctgatca tgatggccag gaaggctccc accgcgcaga gcgtgatgtg
     4561 cgcccgcgcc atgcgccgcc agaacctcag ggcgtccggc cgtaccatca gcccgcgccc
     4621 cgccagccgg tcaccgatct cctgcaccgc cgcgctgcgc atcagttccc ggcgcagcca
     4681 ggtcagggcg ccgtgcgggg agttggcgga cagccgcagc aactcctcct cgacggggcc
     4741 gcgcgcgacc ggttgccgca ccgagaccac gccggggccg ccgatcgcca gccgtccgtc
     4801 cgcgtacatc gtgctgatca cggtgtccgc cacccggccc ggtccgcccg ccaggaacgc
     4861 ggcctccagc aggtcgtacg cctgggcgta taccggcccc gagaccctcc tgcgccgggc
     4921 ccggaccgta ccggtgatga gcaacgccga cgacacggcg accaccgcgt agaccaggcc
     4981 cgcgatcgtg cccatgccgt tcaccgcccc ccgctcagcg cgagccggac ccggcgggcg
     5041 aaccgcgccg ccggatggga cggcaggggc gcgggcccgg accgctcctg ccaccacagc
     5101 gtcagccggc gccgggcggc cgagtcgccg ggccggccgt ccgccagcag atgctcggcg
     5161 aacgacagcg cgtcgcggcg gtagccgccg gtcatcgggc ggccctcggc gtatcgcacg
     5221 aacgccgggc ggtagccggt gcccaggatc tccggcagct ccggggccac cttcgcgatc
     5281 acggacgccc gcttggccgt cagcgccgcg ctctgcaccc gcagccgccg gcggtcgaag
     5341 ccctcgggcg gcggggtgtc cccgaccagc gcggacagca gcgcggtctg ggcgagcgcc
     5401 agccgctcac ggacggcgtc cggcaccggt acctcgggca gcggagcgtc gggcagcggt
     5461 gcctcgggcg cgggggcgtc ggccgcaggg gcgtcggccc cggtgcgggc cttgtcggcc
     5521 tcgtcggcct tgtcggcctt gtcggcctcg tcggccgcgg cggaggcccg caccggggcg
     5581 ggggcctcct ccccggtccc cgccgccgtg gccgcttccg ctgcccccgc cgccgtgacc
     5641 ggcttcgcgc tccccgccgc tgtccccgcc gtcgccatcc ccgccgtcgc catccccgcc
     5701 tccgcggccc ccgccgcccc cgccgccgtc accgcgcgga tcgcggccag ctcgcccgcc
     5761 agctccccgg ccgccgggaa gtcctcgtcg cgctccagca gtacgccggg cggtgccgta
     5821 cgggcgcaca gttcggcgag gacgtcgagt accggctggg tcaccggatg ggcgtggctg
     5881 tcgtgccaca cgccgtcctt ctccaccccg cccgccacat ggacataggc gatcgccgac
     5941 gtgggcagct cgtccagcgc cttggccggg tcctcgcccc ggttgacgtg gttggtgcgc
     6001 agattggcga cgtcgatgag cagccgcacc cccgtgcgct ccacgagctc ggccaggaac
     6061 tgaccctcgg tcatctcctc gccgggccag gagatcagcg ccgcgatgtt ctccaccgcg
     6121 agcggcaccg gcagcgcgtc ctgcgcgatg cgcacgttct cgcacagcac gtccagcgcg
     6181 tcacgcgtcc gcggcaccgg cagcaggtgc ccggcctcga tggacggtga cgcggtcagc
     6241 ggccccccgg ccctgacgaa cgcgatgtgc tcggtgacca gcggcgagcc cagcgcctcg
     6301 gcccgctcgg ccagcgcgcg cagacggccg ggttcgggcc gctcggctcc cccgaggccg
     6361 agcgacaccc catgcgggac caccgtggtc ccgcgctcgc gcagcagccg gagcgcgtcc
     6421 ggcacatggt cggggcagat gttctccgcc accacctcca cccagtcgat gccgggcagt
     6481 cgcgcgatgt catcggcgat ctccggtcgc cagccgattc cggtaccgag gcgcaccatg
     6541 gctccccctc ctcgttccgt accgcgtccc ccgcggtgtg ttctcgcgtg ccggggtgat
     6601 gaccccgtag aggaaggacg aatccccggg cctcgcggtt cagaggttca tttgagcttc
     6661 ggggtgtcac ggagggggtg tacgcccccg gggcggccgc cgacggccgt gcgcacgctc
     6721 accgcacagg cggccatgcc acggccaccg cacaggcggt gcgaatggtc atcgggtggc
     6781 tcgtgtcgct gaatatggtc actgttacaa aaatttgggg ccctgtggcg acagatgttc
     6841 agaggggaaa ataggatcgg tctggtctgg ggggatggaa gatcacacct ttgtgccgcg
     6901 cccacgcgcg gagcgagcgt gggggaaaca cgtgccgggc agcgacaacg ccatggggaa
     6961 gcaccgtaag caccgccgca agccgtcctc gaagagccgg aggatgcgac tgcgccgctg
     7021 ggccgcggcg cggatccggc tggactaccc ccggacgggg cgccgtgggg tggggcggtg
     7081 gctgccgtcg tggcgtcaga tcgccctgtt cttcctgttc tgcgtcggca gtctgaccgg
     7141 tctgctgagc tttctgtatc tccagaccga catccccaag aacctcaacg acttcgccac
     7201 ccagcaggac aacgtctact actgggcgga cggcaccgag atggcccgca ccggtccggt
     7261 caaccggcag gaggtgccgc tgaagaaggt gccggagcag gtccagtggg cgatcctcgc
     7321 cgcagagaac gccagcttct actccgaccg cggggtgtcc ccgagcggac tgatgcgcgc
     7381 ggtgacacgg atggtgacgg gcggtgagac ccagggcggc tccaccatca cccagcagta
     7441 tgtgaagaac gcctatctga accagcgtca gaccttctcc cgcaagctca ccgagatgtt
     7501 catggccatc aagctggacg accggatgag caagcgggac atcctcgagc gctatctgaa
     7561 caccagctgg ttcggccgcg gcagctacgg cattcagcgg gcctcgtacg cgtactacgg
     7621 caaggacgtc tcggaactca acgcgagcga gggcgcgttg ctcgcctcgc tgctcaaggg
     7681 cgccgggaca ttcgatccga cgctcggcgc caagaaccac aagcgggccg tcgaccgctg
     7741 gaagtgggtg ctggaccgga tggtcgacat cggaaggctg tcgaagtccg agcgggcgcg
     7801 ctatacgaag ttccccgagc ccaaggcgcc ccccaagccg gccgggctca ccggtcaggt
     7861 cggctatctg gtggagacgg ccaaggcgta tgtgagctcc cataccgaga tatccgacgc
     7921 cgatttcgac ctgggcggct atcagatcca cacgaccttc gagaagccca aggtcaacgc
     7981 gctcgccaag gcggtggagc agacccggga caagctcgat cccgagcggc gccccgagga
     8041 ccggcatgtg cgggtgggcg ccgcctcggt ggcccccggc ggcgcgatac gcgcgctgta
     8101 cggcgggccc ggctatctcg agcagggatt caacgacgcg aacgcctcca acgtcccggc
     8161 cggcacctcc ttcaccccct tcgtctacgc cgccgcgctg cgcgacggcg tactgctcca
     8221 gcgcaacggg gcacggacgc ccgtgacgcc ctcgacgctc tacgacggca acaacaagat
     8281 cgcggtgcat acgcccgagg ggccgtactg ggaccgcggc ggcaagatcg tgcgggccac
     8341 caacgacggc gacgcctcct atgggcaggt ctcgctgcgc caggcgatcg tgcagtccat
     8401 caacaccccg atgatccagc tgggcatgga tgtcgggctg gaccgggtcc ggcgggtgtc
     8461 catcgacgcg gggctgctgc ccagcagctt cggcgcgcgg gtgcccgcgt tctcgctcgg
     8521 caccgcgacg cccagctcga tccgtatggc cagcgcctac gggacgttcg ccgccgaggg
     8581 cacgcaccac gcgccgtact cggtgagcgg gctcacccac ggcgggcaga aggtcgagct
     8641 ccgtaaggac cgcgccagac gggccttcag cgcggaggtc gcggcacagg tggacgacgc
     8701 gctgcgcggc gcggtccaga gcccggacgg ctcggcgcgc ggggcggcca tggccgatgc
     8761 cgaggtggcc ggtaagggcg gcacggcgca ggacggcacc tccgcctggt tcgtcggcta
     8821 caccaaggag ctctcgacgg cggtctcgct gtcccggctg gaccccaagt cgcaggagct
     8881 gatgccgctc gaggggatgg gcggaggagg cgccgacact tcgggtgaca cctaccccct
     8941 ggacatctgg aagagctata tggcgggccg tgcggcggaa tgagcgacgg cggaagcccg
     9001 gcaacgggac cgcttacctg gtcttggtgt acgtcgacca gtcgggcgac tccgccgggt
     9061 ccagcatccg cagcttctcc aggaccttcg ggtcctgggc gtccagccag tcggcgagct
     9121 gcttgaacga cacgcatcgc actccgtcgc ggcggcagac gctcctcatc acgtcctcga
     9181 tggcctgcat atagatgccg ccgttccagg tctcgaagtg attgccgatg aacagcggcg
     9241 cccggctgcc gttgtaggcg cgctcgaagc cgttcagata gccctggcgg gtcagccgct
     9301 cccactcggc gtacttcgcc ggatcgccct cggtgctgtc accggactgg ttgaagagga
     9361 agttgaagtc catcgagagg acctgcttct cgctctcggg gtacggcacc agctggagcg
     9421 ggaagttcca gatgccgtcg atcttggacg gccagagctg gaagtcgccg ggtgagctcg
     9481 cgtcatagcg ccagccgagg gacttgatcg ccgggatcag cgtcttctgg ccctccaggc
     9541 acggtgcgcg cccgcccacc agctccacgt cgtagtcgaa cggcagcggt tcgatgtcgg
     9601 tgaagccggt gttggtcttc caccgcttca caaaggagta cgcctgctcc gtttcgctct
     9661 tccactcctg ggtggaccag tcctgcccgc ccttggcgcc gcagaagtgt ccgttgaagt
     9721 gggagccgat ctcattgccc tccagccagg caccgcgcag ctgctccagg gtctctctga
     9781 tgtgctcgtc ggtggggaag gagatggcgg ccgagccccg cttgtgctgg ggcggctgat
     9841 agaggtcgct cttcgtcttg ggcagcagat acataccgct gaggaagaac gtcatcgtgg
     9901 cgtcgctctc cttggcgacc tggcggaaac gggagaagag gtggtcgtcg ttctccagcg
     9961 cgccgtccca ggagaagacc acgaactgcg gcggtttctc accgggtttg agcttctcgg
    10021 gcttgggctg gtgcggctgc ggtccggtgt aggacgtgga gccgtccccg agcaccttca
    10081 cctcgccgtc ccaatcggcg tacttgtctt ccttcttggg ctctctgggg acccggtcgg
    10141 acttggggga cgcggcaccg gcggggaggt tcccgtccga cgtgccgccg cccgcgtccc
    10201 ccggcgaggt cagggctatc agcagcccca ccacgacggc cacggccgtc agggcgccta
    10261 tgggcgcggc cacgccatag gcgttcctcg cgcttaaagc ctgcaacgct ttcacgatct
    10321 tcagcgcctt cacgggtccc gccccctggc aacagtacag atgtacgtct ccctcagtat
    10381 cacagaagta tcacgtgctc ttcacatcag tctcctcacc aaccggtcaa caacaggtgg
    10441 ttgacgagca gtgcgagaca cgcctgcgcc agaagccatc ggcggcggtc aacggcgggc
    10501 agcagcgcgg tggtgggcag cagccagagg atgaagggaa gccagatccg ctccgtctcg
    10561 gccttgctca tccctgacag atcggccacg acgagcgcga gcagcgcggc gaaggccagc
    10621 acgacgagcc cgtccgggac gccgcccggg gcgcccggtc cgcccggccg cagtcgccgg
    10681 acggcatccg gcaccgccga cagcatgcgc cgcacacccg cgacacaggc cggaccggcg
    10741 gccaccgtcg cgacggccgg attggcccag acccagtacc cgtacgggcg gtcggcggcc
    10801 acgccctggt agtagcgctc gaccagcagg gagtacccct cccaccactg gaagccggcc
    10861 agggtgaacg caccggccac caccgccacc ccggtcagcg cgaagggcag cggccgcgcg
    10921 gtgcgggcga gcagcagcac gacgacggcg ggcagggcca gcagcgccag cccgtaggag
    10981 agatacaccg tcagccccag cagcagtccg gcgccgaacg cgaccgcccc gggcgcccgg
    11041 accgtacgcg tcgccgcgag cgccagcagc gcgagccccc acgccgcgac ggccgtgaaa
    11101 tagccgtcgg ccgaggcgcc cacccacacc gcccccggcg ccaggaccag gaacggggcc
    11161 gccgcgcgcg cggcccgctc ccgccccagg gcgcgcagcg tgaccagcac cgcggccgcc
    11221 gccgagccgc ccacggtgac gcagaacgcc ccggcccaac cgccgccgcc caggcccagc
    11281 cggtccaggc ccacgaaggt gagcacggcg cccggcggat ggcccgcgat atgggcgggc
    11341 cagtggtcgg gctgagtgag caggatatgg ccggtgaagc cgcgcagggc cgcgccgatg
    11401 tcgtggaacc gatcgacgcc gcgcagatat tcatggcggg ccgtcagccg ttccgccacc
    11461 ccccgctgcc atccgtcgac cagcgccagc gaccagatcc aggccatggc cgccgcccag
    11521 acggctccca gcagcccgc
//
