LOCUS       NZ_CP019458            10405 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces autolyticus strain CGMCC0516 chromosome, complete
            genome.
ACCESSION   NZ_CP019458
VERSION     NZ_CP019458.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces autolyticus
  ORGANISM  Streptomyces autolyticus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10029028)
  AUTHORS   Yin,M., Jiang,M., Ren,Z., Dong,Y. and Lu,T.
  TITLE     The complete genome sequence of Streptomyces autolyticus CGMCC 0516,
            the producer of geldanamycin, autolytimycin, reblastatin and
            elaiophylin
  JOURNAL   J Biotechnol 252, 27-31 (2017)
   PUBMED   28472671
REFERENCE   2  (bases 1 to 10029028)
  AUTHORS   Yin,M., Jiang,M. and Lu,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2017) Yunnan University, Yunnan University, 2#
            Cuihu Beilu, Kunming, Yunnan 650091, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 279x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:05:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,227
            CDSs (total)                      :: 8,141
            Genes (coding)                    :: 7,920
            CDSs (with protein)               :: 7,920
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 74 of 221
            Pseudo Genes (incomplete)         :: 182 of 221
            Pseudo Genes (internal stop)      :: 9 of 221
            Pseudo Genes (multiple problems)  :: 42 of 221
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:31:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3215720
            Orig. end                         :: 3226125
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019458.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="21"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(858..871)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            1332..1934
                     /locus_tag="BV401_RS12620"
                     /old_locus_tag="BV401_12575"
     CDS             1332..1934
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718917.1"
                     /locus_tag="BV401_RS12620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_12575"
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_237288246.1"
                     /transl_table=11
                     /translation="MARLAHLLLGSSDGADDLAADALVAVWHQWDRVRAADHPAAYARG
                     VVANLARSRIRSLVRERRRVALFSSQRSDHVDDPDVSAVVDVQAALVRLPFRKRACVVL
                     RHAFDLSERETARTLGISVGTVKSQTSRGIAELERLLGPPDGEGELPGATDAGRTVRTL
                     RSGNTGPTGTGPTGTGPAGPARHSGQMGHAGHAGGGS"
     gene            1934..2746
                     /locus_tag="BV401_RS12625"
                     /old_locus_tag="BV401_12580"
     CDS             1934..2746
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059804.1"
                     /locus_tag="BV401_RS12625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_12580"
                     /product="hypothetical protein"
                     /protein_id="WP_079257181.1"
                     /transl_table=11
                     /translation="MIEHRERAEGRDVVAELRAAAASHEPDRSRMLARVTAGIAADDRR
                     RNRAPRMGPRRSRMVGPVAGLAAAVAATGLVMLATDGAERPQTVRTSSEPVVPPAGAGA
                     DTRPGEEPGAGSQSEAERHTPSAYPTAAGPNHLAEPTVRSKGTINAHSNPYWAQSDITL
                     TIGRPLTSLTVELRVAETGGVRSTGSWSSLPTSDMTAATHIEDGALVYRWTLRKGATVP
                     AGRFVFAGQYNHAEGERDNGRDAYSASGNGDSGAFAVRGDFPRFTVGD"
     misc_feature    2821..2834
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.61"
                     /tool="antismash"
     misc_feature    2830..2845
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.4"
                     /tool="antismash"
     misc_feature    complement(2894..2909)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    3030..3042
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     gene            3067..3627
                     /gene="ectA"
                     /locus_tag="BV401_RS12630"
                     /old_locus_tag="BV401_12585"
     CDS             3067..3627
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059805.1"
                     /locus_tag="BV401_RS12630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_12585"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_069869914.1"
                     /transl_table=11
                     /translation="MTAAQADLVRSELDLPEDLTLDTPRVEDGAAIWRIARDSKALDLN
                     SSYSYLLWCRDFAATSVVARDADGGPVGFITGYVRPERPETLVVWQVAVDHARRGRGLA
                     ATLLDGLVKRVAATGVRGIETTITPDNTASNRLFTSFAERHQAPLEREVLFHGGLFPDG
                     AHEPEVLYRIGPLGSLGPSADRT"
     gene            3698..4960
                     /gene="ectB"
                     /locus_tag="BV401_RS12635"
                     /old_locus_tag="BV401_12590"
     CDS             3698..4960
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 4.4e-72.
                     Score: 234.8. Matches aSDomain:
                     nrpspksdomains_BV401_RS12635_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="BV401_RS12635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_12590"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_069869915.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.2e-75, bitscore:
                     252.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFEALESEVRSYCRGWPAVFDRAQGSHMYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNHILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTIPDFLWFERLLEDQGSGLNRPAAVIVETVQGEGGINVARP
                     EWLRALAELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGMPMS
                     LALFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWADGQMEKQTLARGEQVELALR
                     AICEEQSGTRFRGRGLVWGLEFDDKPRATAVCRRAFELGLLLETSGPESEVVKLLPALT
                     ISPDDLDEGLRTLARAVRETA"
     aSDomain        3782..4786
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BV401_RS12635_Aminotran_3.1"
                     /evalue="4.40E-72"
                     /label="BV401_RS12635_Aminotran_3.1"
                     /locus_tag="BV401_RS12635"
                     /protein_end="363"
                     /protein_start="28"
                     /score="234.8"
                     /tool="antismash"
                     /translation="FDRAQGSHMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNHILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTIPDFLWFER
                     LLEDQGSGLNRPAAVIVETVQGEGGINVARPEWLRALAELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGMPMSLALFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDTYWADGQMEKQTLARGEQVELALRAICEEQSGTRFRGRGLVWGLEFD"
     gene            5001..5405
                     /locus_tag="BV401_RS12640"
                     /old_locus_tag="BV401_12595"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718913.1"
                     /locus_tag="BV401_RS12640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_12595"
                     /product="ectoine synthase"
                     /protein_id="WP_069869916.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.2e-53, bitscore:
                     177.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEDTERHVRSKSGTWESKRIVLAKEGVGFSLHETILY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHWITPGTMYLLDGHEKHTMRPKTDFR
                     CVCVFNPPITGREDHDENGVYPLITETEEA"
     gene            5410..6297
                     /gene="thpD"
                     /locus_tag="BV401_RS12645"
                     /old_locus_tag="BV401_12600"
     CDS             5410..6297
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718912.1"
                     /locus_tag="BV401_RS12645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_12600"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_079257182.1"
                     /sec_met_domain="PhyH (E-value: 1.5e-39, bitscore: 135.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVTDLYPTRGAVEVATPRVDPVVWSEPGAPGPLQPSELSDFDR
                     DGFLAIDELITPDEVAEYRAELDRMIADPDVRADERSIIEPTSQDVRSVFEVHRISEVF
                     AKLVRDPRVVGRARHILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSVALTENFAHNGSLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDEALSGF
                     ADKHGIRQFTGKPGSAVWFDCNCMHGSGDNITPFSRSNVFIVFNSVENTAVEPFAAPVP
                     RPDFIGARDFTPVR"
     gene            complement(6426..7472)
                     /locus_tag="BV401_RS12650"
                     /old_locus_tag="BV401_12605"
     CDS             complement(6426..7472)
                     /NRPS_PKS="Domain: Aminotran_5 (13-342). E-value: 2.5e-26.
                     Score: 84.4. Matches aSDomain:
                     nrpspksdomains_BV401_RS12650_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180697.1"
                     /locus_tag="BV401_RS12650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_12605"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_079257183.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.4e-20, bitscore:
                     70.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESLAGAEFAPHTTYLTTASSGLIPARSVAAVQAVLDDSAAGQPI
                     VDVAFEAVEAARAAYARLVGVPARRVAAGSSVAVYAGMIATSLPPGAEVLVAEGDFSSL
                     VNPFAVRGDLKLRIVPLEEIAEAVRPGTALVAVSAVQSADGRIADLDAIREAARTHGAR
                     TLVDVSQAAGWFPVNAAEADYTVCVAYKWLMCPRGAAFLTVPEDLGGLTPVFAGWVAGE
                     KPWDSCYGPVEELAHSARRFDESPSVYSYVAARHSLALIEELGTEAIAAHDRALADRFR
                     DGVIGLGHRPIAAPGSAIVSVPGLGDAAPRLAEAEVHVSARAGNLRASFHLYNTAADVD
                     RLLEKLSG"
     aSDomain        complement(6447..7433)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BV401_RS12650_Aminotran_5.1"
                     /evalue="2.50E-26"
                     /label="BV401_RS12650_Aminotran_5.1"
                     /locus_tag="BV401_RS12650"
                     /protein_end="342"
                     /protein_start="13"
                     /score="84.4"
                     /tool="antismash"
                     /translation="TYLTTASSGLIPARSVAAVQAVLDDSAAGQPIVDVAFEAVEAARA
                     AYARLVGVPARRVAAGSSVAVYAGMIATSLPPGAEVLVAEGDFSSLVNPFAVRGDLKLR
                     IVPLEEIAEAVRPGTALVAVSAVQSADGRIADLDAIREAARTHGARTLVDVSQAAGWFP
                     VNAAEADYTVCVAYKWLMCPRGAAFLTVPEDLGGLTPVFAGWVAGEKPWDSCYGPVEEL
                     AHSARRFDESPSVYSYVAARHSLALIEELGTEAIAAHDRALADRFRDGVIGLGHRPIAA
                     PGSAIVSVPGLGDAAPRLAEAEVHVSARAGNLRASFHLYNTAADVDRL"
     gene            complement(7738..9273)
                     /locus_tag="BV401_RS12655"
                     /old_locus_tag="BV401_12610"
     CDS             complement(7738..9273)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059810.1"
                     /locus_tag="BV401_RS12655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_12610"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_232564419.1"
                     /sec_met_domain="Aldedh (E-value: 1.8e-41, bitscore: 141.0,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPVWSVDPRTGKQREQVAVEATAEEVDRVVRAAHAARGSLADR
                     AVRAALLRTAADLLDEARDLLVEAADAETALGPARLTGELARTTYQLRSFATIVDEGGF
                     LDVRIDHADATQTPPWPDLRRYKLPLGVVAVYAASNFPLAFSVPGGDTASALAAGCPVI
                     VKAHPDHPATSEVAASALRRAAERVGVPAEVIAVVHGFDAGIELVRHPLIAAAGFTGSV
                     RGGRALHDAAASRPVPIPFHGELGSLNPVVVTEAAAAERGDQIGAGLAGSMTLGVGQFC
                     TKPGFVLAPAGPAGDGLVKSLTEAVSNTEAGVLLDHRMRDNFVAGVQERAELADVEAPV
                     TPGAGGEHTVSAGFLTVPAGRLAESGPHDALLEECFGPVTVVARYESDEEISAVLGRLP
                     GNLTATIHLSAAEAEGTEGRAGELIAELTPLAGRVLVNGWPTGVAVAPAQHHGGPYPAT
                     TSTSTSVGGTAIERWLRPVAYQDTPAALLPPELRDENPLELPRRINGVREQRG"
     gene            9409..10179
                     /locus_tag="BV401_RS12660"
                     /old_locus_tag="BV401_12615"
     CDS             9409..10179
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="BV401_RS12660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BV401_12615"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_069869920.1"
                     /transl_table=11
                     /translation="MSAAETGGAQVKSAVRTVELLEFFAGRPGMHSLAAVQEAVGYPKS
                     SLYMLLRTLVELGWVETDATGTRYGIGVRALLVGTSYIDGDEVVAAARPTLDRLSDDTT
                     ETIHLARLDGTNIVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATYSDEQVRKLL
                     PETLSQLTEHTITDREKLIEELHLVREQGYAVDREENTLGLRCFGIAIPYRTPSRDAIS
                     CSVPVARLTPAHEQMIKDALFDARDRLTLATRRL"
ORIGIN
        1 gggccaggcc gccgcccgcg tcggtgccgg tgtagatcgg gatgcccgcg tcgtaggccg
       61 cccgcaccgt ctcgtaccgg cgctcgtgca gccgccgcat atggtcggcc cagcgcggga
      121 acctcgcctc gccgcccagc gccagccgcg ggaaggtggc gatgttgacc agggtcggga
      181 cgatcgccac gccccgctcg gcgaacagcg ggatggtctc ctcggtgagc ccggtggcgt
      241 gctcgatgca gtcgatcccc gcctcgacca gcggggccag gctctcctcg gcgaaacagt
      301 gcgccgtgac ccgcgcgccc agccggtgcg cctcggcgat ggccgcctcg accgcgccgc
      361 gcggccagca ggaccccagg tcaccggtct cccggtcgat ccagtcgccg accagcttga
      421 cccagccgtc gccgcgccgc gcctccgcgg ccacataggc gaccaggtcc tcgggctcga
      481 tctcatacgc gtagttgcgg atgtagcgcc tggtacgggc gatatggcgg ccggcccgga
      541 tgatgcgggg cagatcctcg cggtcgtcga tccagcgggt gtcggccggc gatcccgcgt
      601 cccgcagcag cagcgccccg gcgtcacggt cggtcagcgc ctgtttctcg ctggtggagt
      661 cgtccaccgc gccatgggcg tcgaggccga catggcagtg ggcgtcgacg agtccgggca
      721 gcacccagcc ctcgatcagc cggatgtccc tggccccggc cggccgctcg aacgtgatcc
      781 ggccgtccac cacccacagc tcgtcccgga tgtccccggc agcgtcgccg gtgtccggtc
      841 cgaccaggac ccggcccttg atatggagca ccgcgctatc cctcatgcaa cgcactgtac
      901 gagaccgggg cagggcgttg accggccaat tcccaggagc cggaacggcg ccactcgaac
      961 aacttgcgca gcgctgctac gctcacgcaa cccaccggac gagcggcagt gaggaggtcc
     1021 tcgtgagcgc tccgaccgat gacgcggccg ccgcgagcac cgcgcgagcc gtaccgatgg
     1081 acacgacgca cacgacggac ccaccacggg ccgcagcacc ggaggcgccc gacgcgccgg
     1141 agggccccga cggcttatcg gccgacgccc accccgactt accggccgac ttaccggccg
     1201 actcaccggc cactgggcac cccgacccac cggccgcacc gcacgccgac ttacagaccg
     1261 ccgccccgca ccccgaccca cccgacccac aagccgagtt ccgggacttc ttcgagcggc
     1321 accacaccga gctggcccgc ctcgcccatc tgctgctcgg cagctccgac ggggcggacg
     1381 acctggcggc cgacgcgctg gtggcggtct ggcaccagtg ggaccgggtg cgggccgccg
     1441 accatccggc cgcgtacgcc cggggcgtgg tcgccaatct ggcccgctcc cggatccgca
     1501 gcctggtgcg cgagcggcgc cgggtggcgc tcttctcctc gcagcgctcc gaccatgtgg
     1561 acgaccccga tgtgtccgcc gtggtggatg tgcaggccgc gctggtccgg ctgccgttcc
     1621 gcaagcgggc gtgtgtggtg ctgcggcacg cgttcgacct ctccgagcgg gagacggccc
     1681 gtacgctggg catttcggtg ggcacggtca agagtcagac ctcccgcgga atcgccgagc
     1741 tggagcggct gctcggccct ccggacggcg agggcgagct gcccggggcg acggacgcgg
     1801 ggcggacggt acggacgctc cgctccggga acacaggtcc cacgggcaca ggtcccacgg
     1861 gcacaggccc cgcgggcccc gcgcgtcact cgggtcagat gggccacgcc gggcacgcag
     1921 gagggggcag ttgatgatcg agcatcgcga gcgcgccgag ggccgcgatg tcgtcgccga
     1981 gctgcgcgcg gccgccgcct cccatgaacc ggaccggagc aggatgctgg cgagggtcac
     2041 ggcgggcatc gcggccgatg accgacgccg gaaccgggcc ccgcggatgg gcccgcgccg
     2101 gtcccggatg gtgggcccgg tcgccgggct ggcggcggcg gtcgccgcga cgggcctcgt
     2161 gatgctggcc acggacggtg ccgagcggcc gcagacggtg cgcacctcga gcgaacccgt
     2221 cgtgcccccg gcgggcgcgg gcgcggacac acggcccggg gaggagccgg gcgccgggtc
     2281 gcagagcgaa gctgagcggc acacgccgag cgcctatccc accgcggccg gtccaaacca
     2341 cctcgccgag ccgaccgtac ggtccaaggg caccatcaac gcccattcca acccttactg
     2401 ggcgcagagc gacatcacgc tcaccatcgg caggccgctg acctcgctga cggtggagct
     2461 gcgggtcgcg gagaccgggg gtgtgcggtc caccggctcc tggagctcgc tgccgacctc
     2521 cgatatgacc gccgccacgc atatcgagga cggtgcgctg gtctaccgct ggacgctccg
     2581 gaagggtgcg accgtacccg cgggccggtt cgtcttcgcg gggcagtaca accacgccga
     2641 gggcgagcgg gacaacggcc gggacgccta ctcagcctcg ggcaacggtg actccggggc
     2701 cttcgcggtg cggggcgact tcccccggtt caccgtggga gattgaattc atggcagctt
     2761 ggccagaaat tctccgcgtt tttccgctac tgcttccaca ttcgtctgcc cgcttttgtg
     2821 attcctaaac acaaaatttt ttcacgggat cctgatcccg taattcaaga cagtcaccgg
     2881 gcgattacgc acatgtgacg gaccacacat gagattcact ttgccccggt atctccccca
     2941 caaatcagcc tctttcggcg tgcggccgcg cgcacgcgcg ctcccgcgtg ataacacgac
     3001 cacccccttt cgcgtaaccc cccaccgcga tgcattttcc gattctgctg ggtaaattga
     3061 atttgcatga ccgccgcaca agcagacctt gtccgtagcg aattggattt gccggaggac
     3121 ctgaccctcg acaccccgcg cgtcgaggac ggagccgcga tctggcgaat cgcacgcgac
     3181 tccaaagccc tggacctcaa ctcctcctac agctacctcc tgtggtgccg cgacttcgcc
     3241 gccacatccg tagtggcgcg cgacgccgac ggcggaccgg tcggcttcat caccggctac
     3301 gtccgccccg agcgccccga gaccctggtc gtctggcagg tcgccgtcga ccacgcccgg
     3361 cgtggccgcg gactggccgc caccctgctg gacgggctgg tcaagcgggt cgccgcgacg
     3421 ggcgtccgcg gcatcgagac gacgatcacc ccggacaaca ccgcctcgaa ccggctgttc
     3481 acctcgttcg ccgagcggca tcaagcgccg ctcgagcgtg aggtgctctt ccatggcgga
     3541 ctgttccccg acggtgcgca cgagcccgag gtgctctacc gcatcggtcc gctcggctcc
     3601 ctcgggccga gcgccgaccg gacctgaccc ccacctcgat ccccctcgaa ccctcaccct
     3661 cctcaccccc ctcacacccc tctcccagga gaacgctgtg accatcaccc cgcccgccct
     3721 gagcgtcttc gaggccctgg agtcggaggt gcgcagctac tgccgtggct ggcccgctgt
     3781 cttcgaccgc gcgcagggca gccacatgta cgacgaggac ggccacacgt acctcgactt
     3841 cttcgccgga gccgggtcgc tcaactacgg ccacaacaac ccggtgctga aacgggcgct
     3901 gatcgactac atcgagcgtg acggcgtcac gcacggcctg gacatgtcca ccacggccaa
     3961 gcgcgcgttc ctcgaatcgt tccagaacca catcctgcgc ccgcgcgatc tgccctacaa
     4021 ggtcatgttc ccgggcccga ccggcaccaa cgccgtcgag gccgccctga agctggcccg
     4081 taaggtcaag ggccgcgagg ccatcgtctc cttcaccaac gccttccacg gcatgtcgct
     4141 gggctcactg gccgtgaccg gcaacgcctt caagcgcgcc ggtgcgggca tcccgctggt
     4201 ccacggcacc ccgatgccgt tcgacaacta cttcgacggc acgatcccgg acttcctgtg
     4261 gttcgagcgg ctgctggagg accagggctc cggtctcaac cggcccgccg cagtgatcgt
     4321 cgagaccgta cagggcgagg gcggcatcaa cgtggcgcgg cccgagtggc tgcgcgccct
     4381 ggccgagctg tgcgagcgcc aggacatgct gctgatcgtc gacgacatcc agatgggctg
     4441 cggccgcacg ggcgccttct tctccttcga ggaggcgggc atcaccccgg acatcgtgac
     4501 cgtctccaag tccatcagcg gctacggcat gccgatgtcg ctcgccctct tcaagcccga
     4561 gctggacatc tgggagcccg gtgagcacaa cggcaccttc cgcggcaaca accccgcgtt
     4621 cgtgaccgcc gccgcgaccc tggacaccta ctgggccgac ggccagatgg agaagcagac
     4681 cctggcccgc ggcgagcagg tcgagctggc cctgcgcgcc atctgcgagg agcagtccgg
     4741 cacccggttc cgcggccgcg gcctggtgtg ggggctggag ttcgacgaca agccgcgcgc
     4801 cacggcggtc tgccggcgcg ccttcgagct ggggctgctg ctggagacgt ccggccccga
     4861 gagcgaggtc gtcaagctgc tgcccgcgct gacgatctcc cccgatgacc tggacgaggg
     4921 gctgcggacc ctcgcccgcg cggtccgcga gaccgcatga tcctcccgca ccgcagagaa
     4981 gagaaaggca agaactcacc gtgatcgtcc gatccttcaa ggacatcgag gacaccgaga
     5041 ggcatgtgag gtccaagtcc ggcacctggg agagcaagcg catcgtgctc gccaaggagg
     5101 gcgtcggctt ctcgttgcac gagaccatcc tctacgcggg caccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcg gtgctgtgcg tcgagggcga ggccgagctc accaacgacg
     5221 agaccggtga gaagcactgg atcacgcccg gcaccatgta tctgctcgac ggacatgaga
     5281 agcacacgat gcgccccaaa acggatttcc ggtgcgtctg tgtattcaac ccgccgatca
     5341 ccggacggga ggaccatgat gagaacggcg tatacccgct gatcaccgag accgaggagg
     5401 catgaaccga tgaccgcggt cactgacctg tacccgaccc gtggggctgt ggaggtggcg
     5461 acaccgcgcg tggaccccgt ggtgtggtcc gagcccggag ccccagggcc gctgcaaccg
     5521 tccgagctga gcgatttcga tcgcgacggc ttcctggcga tcgatgagct gatcactccg
     5581 gatgaggtgg cggaataccg cgccgaattg gaccggatga tcgccgatcc ggacgtgcgc
     5641 gccgacgaac gctcgatcat cgagccgacg tcgcaggacg tacggtcggt gttcgaggtg
     5701 caccggatca gcgaggtctt cgccaaactg gtgcgcgacc cgcgtgtggt gggccgcgct
     5761 cggcacatcc tcggctcgga cgtctacgtc caccagagcc ggatcaatgt caagccgggc
     5821 tttggcgcct cgggcttcta ctggcactcg gacttcgaga cctggcacgc cgaggacggg
     5881 ctgccgaaca tgcggaccgt gtcggtctcg gtcgcgctca ccgagaactt cgcacacaac
     5941 ggcagcttga tgatcatgcc gggctcgcac cgcaccttcc tgggctgtgc gggcgcgacg
     6001 ccgaaggaca actacaagaa gtcgcttcag atgcaggacg cgggcacccc gtccgacgag
     6061 gcgctcagcg gcttcgccga caagcacggc atcagacagt tcaccggcaa gcccggctcg
     6121 gcggtctggt tcgactgcaa ctgcatgcac gggtccggcg acaacatcac gccgttctcc
     6181 cgcagcaatg tgttcatcgt gttcaacagc gtggagaaca ccgcggtcga gccgttcgcc
     6241 gcgccggtcc cccggccgga cttcatcggt gcgagggact tcacgcccgt acggtgacaa
     6301 ccgccgtgcg ctgatgtgac gtccgtgatc tgagggaagc cggagtgccc cggcttccct
     6361 cagatttttc cccatctgcc ccgcccgcct gcttggcccg tcaggcttgg ccgcggggag
     6421 ccgactcagc cggacagctt ctccagcagc cggtccacat cggctgcggt gttgtagagg
     6481 tggaaggagg cgcggaggtt accggcccgc gccgagacat ggacctccgc ctccgccagc
     6541 cggggcgcgg cgtcgcccag gccgggcacg gagacgatgg ccgagccggg ggcggcgatg
     6601 ggccggtggc cgagtccgat gaccccgtcc cggaagcggt cggcgagcgc gcggtcatgg
     6661 gcggcgatgg cctcggtgcc caactcctcg atcagcgcca gggagtgccg ggccgccaca
     6721 taggagtaga cgctagggct ctcgtcgaag cggcgggcgg agtgggcgag ttcctccacc
     6781 gggccgtagc agctgtccca gggcttctcc cccgcgaccc agcccgcgaa gacgggggtg
     6841 agtccgccga ggtcctccgg cacggtcagg aaggcggcac cgcgcgggca catcagccac
     6901 ttgtaggcca cgcagacggt gtagtcggcc tcggcggcgt tcaccgggaa ccagccggcc
     6961 gcctggctga cgtccaccag ggtgcgggcc ccatgggtcc gggccgcctc ccggatggcg
     7021 tcgagatcgg cgatccgccc gtccgccgac tggaccgcgc tcacggccac cagcgccgta
     7081 ccgggccgta ccgcctccgc gatctcctcc agcgggacga tgcgcagctt gaggtcgccg
     7141 cggacggcga aggggttgac cagggagctg aagtcgccct cggccaccag gacttcggcg
     7201 ccgggcggca gcgaggtggc gatcatcccc gcgtacacgg cgacggagct gcccgccgcg
     7261 acccgccggg ccggtacgcc gaccagccgg gcgtaggcgg cacgggccgc ctcgaccgcc
     7321 tcgaaggcca cgtcgacgat gggctgtccg gcggccgagt cgtcgaggac cgcctgcacg
     7381 gcggctacgg agcgtgcggg gatcagaccg ctggacgcgg tggtgagata cgtcgtgtgt
     7441 ggcgcgaatt cggcgcccgc caggctctcc atgtccaacc actatggaac gcgctcaccc
     7501 cggcgtccat cgcgatttct tgtgggatgc cttcaagcga tccttatgca tccccgctga
     7561 cctgcggcga ttcctccacg tggcacggca ctcgggtgaa cacaccccgc ggatcggccg
     7621 catcaggccg accgcctcag gccccgtgcg ccgtacgcgg ccgcgggcat gccgaaacgg
     7681 ccgccggaca cgcgggtcag gtccgcggat ccggcggccg tgccgtcgtt ccgggcgtca
     7741 gccgcgctgc tcccggacgc cgttgatccg ccgcggcagc tccagcgggt tctcgtcgcg
     7801 cagctccggc ggcagcaggg cggccggggt gtcctggtag gcgacgggcc gcaaccagcg
     7861 ctcgatggcg gtgccgccga ccgaggtgga ggtggaggtg gtggccgggt agggcccgcc
     7921 gtggtgctgg gcgggggcga ccgctacacc ggtcggccag ccgttgacca ggacccggcc
     7981 ggccagcggg gtgagctcgg cgatcagctc accggcccgg ccctcggtgc cctcggcctc
     8041 ggcggcggag aggtggatag tggcggtgag gttgcccggg agccggccga gcaccgcgct
     8101 gatctcctcg tcgctctcgt agcgcgccac gacggtcacc gggccgaagc actcctccag
     8161 cagcgcgtcg tgcgggccgc tttcggccag ccgccccgcc gggacggtca ggaagcccgc
     8221 gctgaccgtg tgctcaccgc cggcgccggg ggtgaccggg gcctccacgt ccgccagctc
     8281 ggcgcgctcc tggaccccgg cgacgaagtt gtcccgcatc cggtggtcga gcaggactcc
     8341 ggcctcggtg ttgctgaccg cctcggtgag ggacttgacc aggccgtcgc ccgccgggcc
     8401 ggccggggcc aggacgaagc cgggcttggt gcagaactgg ccgacaccca gcgtcatgga
     8461 gcccgcgagc cccgcgccga tctggtcgcc gcgctcggcc gccgcggcct cggtgaccac
     8521 gaccgggttg aggctgccga gctcgccgtg gaaggggatc ggcaccgggc gggaggccgc
     8581 cgcgtcgtgc agggcgcggc cgccgcggac cgatccggtg aaaccggcgg cggcgatcag
     8641 cggatgcctg accagctcga tgcccgcgtc gaagccgtgc accaccgcga tcacctcggc
     8701 gggcaccccg acccgctcgg cggcgcggcg cagcgcggag gcggccacct cggaggtggc
     8761 ggggtggtcg gggtgggcct tgacgatgac cgggcagccg gcggcgaggg cgctcgcggt
     8821 gtcgccgccg ggcacggaga aggcgagcgg gaagttgctg gcggcgtaga cggcgacgac
     8881 gccgagcggc agcttgtagc ggcgcagatc gggccagggc ggggtctggg tggcgtcggc
     8941 gtggtcgatg cggacgtcga ggaagccgcc ctcgtccacg atggtcgcga aggaccgcag
     9001 ctggtaggtg gtgcgggcca gctcgcccgt gagccgggcg gggccgagcg cggtctccgc
     9061 gtcggcggcc tcgaccagca ggtcacgggc ctcgtcgagc aggtcggctg ccgtgcgcag
     9121 caaggcggcg cgcaccgcgc ggtcggccag ggaaccgcgg gcggcatggg cggcgcgcac
     9181 cacgcggtcg acctcctccg ccgtggcttc gaccgctacc tgctcgcgct gctttccggt
     9241 tcgggggtcg acgctccaca ctggtgctga caccgcggat tcctcctgct gtggtgttac
     9301 cgctgcttgc gtctcacaca tcgacggtgt tcgatatgct gaacgctgtc ccgggtgatg
     9361 aatttccccc tgggactcta tggctggtcg aacagagggg tcaaggcgat gtcagctgcc
     9421 gagacgggtg gggcccaggt caagtcggcg gtccgcaccg tcgaactgct ggagttcttc
     9481 gccgggcggc ccggtatgca cagcctcgcc gcggtgcagg aggccgtggg ctatcccaag
     9541 tcgagcctct acatgctgct gcgcaccctg gtggagctcg gctgggtgga gaccgacgcc
     9601 accggcaccc gctacggcat cggggtgcgc gccctgctcg tcggcacctc ctacatcgac
     9661 ggtgacgagg tcgtggccgc ggcccgcccc accctggacc ggctctcgga cgacaccacc
     9721 gagaccatcc acctcgcccg gctcgacggc accaatatcg tctatctcgc cacccggcag
     9781 tcgcagcact atctgcgccc cttcacccgc gtcgggcgcc ggctgcccgc ccactccacc
     9841 tcgctgggca aggcgctgct ggccacgtac tccgacgagc aggtgcgcaa gctgctgccg
     9901 gagacgctga gccagctcac cgagcacacc atcaccgacc gcgagaagct gatcgaggag
     9961 ttgcacctcg tccgggagca ggggtacgcg gtggaccgcg aggagaacac cctgggcctg
    10021 cgctgcttcg gcatcgccat cccgtaccgc accccctcgc gcgacgccat cagctgctcg
    10081 gtgccggtgg cccggctcac ccccgcccat gagcagatga tcaaggacgc cctgttcgac
    10141 gcccgggacc ggctcaccct cgcgacgcgc cgcctctgac ccacgggagg acggccgcct
    10201 ccgccatgcg gcggaggggg atcgtcgggc ggggtgaggc gccgcgccca ccgcgccggg
    10261 aggctggtga ccatgaccca caccctcccc ggcccccgga cgcgctccgc acccgcccgc
    10321 ctgcccggtg acctgaccgc acggatcctg cgggccgtcg cgatcctcgc ctgtctcccc
    10381 tacatcggcc tcaaggccgc ctgga
//
