LOCUS       NZ_CP015588            10405 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces alfalfae strain ACCC40021 chromosome, complete genome.
ACCESSION   NZ_CP015588
VERSION     NZ_CP015588.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces alfalfae
  ORGANISM  Streptomyces alfalfae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8625867)
  AUTHORS   Gu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2016) Institute of Agricultural Resources and
            Regional Planning, Chinese Agricultural Academy of Sciences, 12
            Zhong Guan Cun South Street, Beijing 100081, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04; Celera Assembler v. 8.0;
            GapCloser v. 1.12
            Genome Coverage                   :: 310.8
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:19:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,535
            CDSs (total)                      :: 7,444
            Genes (coding)                    :: 7,210
            CDSs (with protein)               :: 7,210
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 107 of 234
            Pseudo Genes (incomplete)         :: 177 of 234
            Pseudo Genes (internal stop)      :: 26 of 234
            Pseudo Genes (multiple problems)  :: 73 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:53:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6513047
            Orig. end                         :: 6523452
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015588.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="26"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    976..991
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
     gene            1423..2031
                     /locus_tag="A7J05_RS28275"
                     /old_locus_tag="A7J05_27575"
     CDS             1423..2031
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="A7J05_RS28275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_27575"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_062771837.1"
                     /transl_table=11
                     /translation="MDLDLPQLPFSLRGYGPDGHWSHEDGVLTGWAGARQDRFVPPTGE
                     ALDPAADAPRLLGAPEGDFQLIARVTVGFAAPFDAGVLYLHVGEREWAKLCLERSPDEP
                     TVCTVVTRGHSDDANAFTVDGASAWLRISRTGSAFAFHASADGERWTFVRIFSLGDEES
                     AGAALVGFMAQSPVGEGCVVTYDHIEFRPNWPKGLRDGS"
     gene            2146..2862
                     /locus_tag="A7J05_RS28280"
                     /old_locus_tag="A7J05_27580"
     CDS             2146..2862
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067467.1"
                     /locus_tag="A7J05_RS28280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_27580"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_076686874.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDAVEVVHRSFELDP
                     GRAKGDTGQVLPMLMNKYGMTEEQAREGERRLGENAAAEGLDYRIEGRDHGSTFDMHRV
                     LHLAKERGRQDELIGLLYRANFAEERSVFADDERLVELAAEAGLDEAEVRAVLADPGRY
                     ADDVRADEREAAELGANGVPFFVLDRRYGVSGAQPAEVFTQALEQAWGGRSPLTTLADA
                     GDGEACGPDGCAVPGA"
     gene            2885..3946
                     /locus_tag="A7J05_RS28285"
                     /old_locus_tag="A7J05_27585"
     CDS             2885..3946
                     /NRPS_PKS="Domain: Aminotran_5 (125-347). E-value: 1.1e-22.
                     Score: 72.4. Matches aSDomain:
                     nrpspksdomains_A7J05_RS28285_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156955.1"
                     /locus_tag="A7J05_RS28285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_27585"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_076686875.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 4.1e-07,
                     bitscore: 28.3, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 2.2e-17, bitscore:
                     61.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSLGIDHHVSAEFAPETTFLNTAVRGLLPRRAAEAVQALTAELV
                     SGGRSGSGDFATVDRARASFARLVGVDPSRVATGGSVAVHVGLIAASLPAGAEVLFPEG
                     DFSSLINPFVLRGDLKPRFAPLDELADAIRPKTALVAFSTVQSADGRTADCAAIREAAA
                     AHGARTLADTTQSAGWQPLDAGAYDYSVTGAFKWLLCPRGTSFLTVGEAAQESLAPLHG
                     GWLAAADAWNSTYGPIGEVVADARRFDEPPAFLAHHGAAAALSLIEEIGVDAIHRHDLA
                     LAARYRAGLAGLGHAPVPGASPIVSVPGLADREPELARAGVVVAARAGHLRATFHLYNT
                     EADVDRLLDVLAG"
     aSDomain        3260..3925
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A7J05_RS28285_Aminotran_5.1"
                     /evalue="1.10E-22"
                     /label="A7J05_RS28285_Aminotran_5.1"
                     /locus_tag="A7J05_RS28285"
                     /protein_end="347"
                     /protein_start="125"
                     /score="72.4"
                     /tool="antismash"
                     /translation="LDELADAIRPKTALVAFSTVQSADGRTADCAAIREAAAAHGARTL
                     ADTTQSAGWQPLDAGAYDYSVTGAFKWLLCPRGTSFLTVGEAAQESLAPLHGGWLAAAD
                     AWNSTYGPIGEVVADARRFDEPPAFLAHHGAAAALSLIEEIGVDAIHRHDLALAARYRA
                     GLAGLGHAPVPGASPIVSVPGLADREPELARAGVVVAARAGHLRATFHLYNTEADVDRL
                     "
     gene            complement(4027..4926)
                     /gene="thpD"
                     /locus_tag="A7J05_RS28290"
                     /old_locus_tag="A7J05_27590"
     CDS             complement(4027..4926)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848585.1"
                     /locus_tag="A7J05_RS28290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_27590"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_062771846.1"
                     /sec_met_domain="PhyH (E-value: 1.5e-38, bitscore: 132.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTTDLYPTRGAEEVLIERKDPVVWSEPGAEGPISAGDLA
                     GFERDGFLAIDQLITPDEVSVYHAELERLVNDPAMRADERSIVEPRSQEIRSLFEVHKI
                     SELFGKLAADPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTKNHDTNGGLMIMPGSHKTFLGCAGATPKDNYKRSLQMQDAGIPSNET
                     LTHLASDHGIRLFTGEAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENTAVEPFA
                     APVRRPEFIGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="A7J05_RS28295"
                     /old_locus_tag="A7J05_27595"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536875.1"
                     /locus_tag="A7J05_RS28295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_27595"
                     /product="ectoine synthase"
                     /protein_id="WP_062771849.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.4e-52, bitscore:
                     175.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAASGTWESKRIVLAKERVGFSVHETILY
                     AGTETSMWYANHIEAVVCTKGEAELTDDTTGEKYTITPGTMYLLDGHEKHTMRIKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEPDEA"
     gene            complement(5459..6730)
                     /gene="ectB"
                     /locus_tag="A7J05_RS28300"
                     /old_locus_tag="A7J05_27600"
     CDS             complement(5459..6730)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 2.1e-72.
                     Score: 235.9. Matches aSDomain:
                     nrpspksdomains_A7J05_RS28300_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="A7J05_RS28300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_27600"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_076686876.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.7e-77, bitscore:
                     257.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMFDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGIAHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGRTPDFIWFERLLEDQGSGLNQPAAVIVETVQGEGGINVARA
                     EWLRKLADVCERWDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTAAAALEAYWTDGSAMEKQTRARGEQVEQVL
                     ASIVEENRADIKEFRGRGLVWGIEFLDKDRASAVAKRAFELGLLIETSGPEGEVVKLLP
                     ALTITPEELDEGLRTLARAVRETA"
     aSDomain        complement(5636..6646)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A7J05_RS28300_Aminotran_3.1"
                     /evalue="2.10E-72"
                     /label="A7J05_RS28300_Aminotran_3.1"
                     /locus_tag="A7J05_RS28300"
                     /protein_end="365"
                     /protein_start="28"
                     /score="235.9"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGIAHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGRTPDFIWFER
                     LLEDQGSGLNQPAAVIVETVQGEGGINVARAEWLRKLADVCERWDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TAAAALEAYWTDGSAMEKQTRARGEQVEQVLASIVEENRADIKEFRGRGLVWGIEF"
     gene            complement(6801..7352)
                     /gene="ectA"
                     /locus_tag="A7J05_RS28305"
                     /old_locus_tag="A7J05_27605"
     CDS             complement(6801..7352)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059805.1"
                     /locus_tag="A7J05_RS28305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_27605"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_076686877.1"
                     /transl_table=11
                     /translation="MTAAQADLPTDLQAKFLEMPEGLRIDSPAVADGAAIWRIARDSKV
                     LDLNSSYSYLLWCRDFAATSVVARGADGEPVGFITGYFRPERPDTLLVWQVAVDHAQRG
                     RGLAGALLDGLTAKVTAGRAPITVETTITPGNTASERLFLSYARRHGAAVEQKILFDAG
                     LFPDDGHEPEVLYRIGPVGR"
     misc_feature    complement(7361..7375)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.38"
                     /tool="antismash"
     misc_feature    complement(7508..7523)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7683..7699
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.98"
                     /tool="antismash"
     misc_feature    complement(7686..7702)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.98"
                     /tool="antismash"
     gene            complement(7848..8375)
                     /locus_tag="A7J05_RS28310"
                     /old_locus_tag="A7J05_27610"
     CDS             complement(7848..8375)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309984.1"
                     /locus_tag="A7J05_RS28310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_27610"
                     /product="DinB family protein"
                     /protein_id="WP_076686878.1"
                     /transl_table=11
                     /translation="MTTLPDGRRIPPLTADEPAMLAGWLDLHRDTLAVKCAGLDDEQTL
                     RACVKPSALSLFGLVQHLAEVERNWIQRVFAGRDVPLLYGMPPGGFAVDGSRTFAEVLA
                     VWEREVAVSREICAGRDLDETGRLGPEQRVVTGGEAVVSLRWILVHLIEEYARHNGHAD
                     LIRERIDGVTGT"
     gene            complement(8460..9560)
                     /locus_tag="A7J05_RS28315"
                     /old_locus_tag="A7J05_27615"
     CDS             complement(8460..9560)
                     /NRPS_PKS="Domain: Aminotran_5 (17-296). E-value: 3.1e-29.
                     Score: 94.0. Matches aSDomain:
                     nrpspksdomains_A7J05_RS28315_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644018.1"
                     /locus_tag="A7J05_RS28315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_27615"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_237283580.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.2e-22, bitscore:
                     78.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAQHFAAIEARVARLLGTGQDVVIMQGEALLPLE
                     GCIRSAARPGTTALNVITGPYGQTFGNWLRDCGADVVDLAVPFHTAVTAEQVRRALAER
                     PEIDFVSLVHAEAATGNTNPVAEIGAVVREHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWQRIAANPRAPRRSYLSLLDWKERWIDAGRKALLHAP
                     AQLEMLALEACVERIETEGLDALMARHATAAAATRAGALALGGGLRPYVHEAADAAPVA
                     TTLRAPEGVDASDLVAAALAADPVLPLVAGGGALAQEMIRVNHYGPDATRGVVHASLAA
                     LGAALGEAGVTVDLEGARRAVTDAWS"
     aSDomain        complement(8673..9509)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A7J05_RS28315_Aminotran_5.1"
                     /evalue="3.10E-29"
                     /label="A7J05_RS28315_Aminotran_5.1"
                     /locus_tag="A7J05_RS28315"
                     /protein_end="296"
                     /protein_start="17"
                     /score="94.0"
                     /tool="antismash"
                     /translation="AIEARVARLLGTGQDVVIMQGEALLPLEGCIRSAARPGTTALNVI
                     TGPYGQTFGNWLRDCGADVVDLAVPFHTAVTAEQVRRALAERPEIDFVSLVHAEAATGN
                     TNPVAEIGAVVREHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAVS
                     VSERAWQRIAANPRAPRRSYLSLLDWKERWIDAGRKALLHAPAQLEMLALEACVERIET
                     EGLDALMARHATAAAATRAGALALGGGLRPYVHEAADAAPVATTLRAPEGVDASDLV"
ORIGIN
        1 cagcccccgc cctggccgac cgcgcggtgc gcaccgcctt cctgcgcacc gccgccgacc
       61 tcctccagga ggccggggac cggctcgtgg aggccgcgga cgcggagacc gcgctcggcc
      121 cggtccggct caccggggaa ctcgcccgca cctgcttcca gttgcgcgcc ttcgcggaca
      181 tcgtcgacga gggcgccttc ctcggagtcg tcatcgacca ccccgacggg tcggcgaccc
      241 cgcccatccc cgacctgcgc cgctacaaga tccccctcgg cgtcgtcgcc gtctactccg
      301 cgtccaactt ccccttcgcc ttctccgtgc ccggcggcga caccgcgagc gcgctcgccg
      361 cgggctgccc ggtcgtcgtc aaggcccacc ccgaccaccc cggcacctcc gagctggtcg
      421 cctccgtgct gcgcagggcc gccgcccggc acgacatccc cgagggcgtc gtcggcctgg
      481 tgcacggctt cgaggcgggc atcgagctgg tcaagcaccc gctggtggcc ggggccgggt
      541 tcaccggctc catccgcggc ggccgggccc tcttcgacgc ggcggcggcc cggccagtgc
      601 cgatcccctt ccacggcgag ctgggctccc tcaaccccgt cgtcgtcacc gaggccgccg
      661 ccgccgagcg cggcgaggag atcggcaccg gcctcgcggg ctcgatgacg ctcggcgtcg
      721 gccagttctg cgtgaagccc ggcctcgtcc tcgcccccga gggcgcgggc ggcgaccggc
      781 tcgtgaagtc cctgaccgat gcggtcggcg acaccgacgc cggagtgctc ctggaccacc
      841 ggatgcggga caacttcgtc gccggggtcc gcgagcgcgc gggcctcgcg gacgtcgact
      901 cccccgtcac gcccggcgcg ggcggcgagc acaccgtgag cgcgggcttc ttcaccgtcc
      961 ccgcggcacg gctcgccgcc gagggcgagc acgacctgct catcgaggag tgcttcggcc
     1021 ccgtcaccgt cgtcgcgcgc tacaaggacg ccgcggagat caccgccgtc ctctcccgcc
     1081 tgcccggcaa cctcaccgcc accgtgcagc tctcctccga cgaggcggcg ggcgtgagcc
     1141 cccgcggcac cgaactcctg gccgaggtca cgccgctcgc gggccgcgtc ctcgtcgacg
     1201 gctggcccac cggcgtcgcc gtcgcccccg cccagcacca cggaggcccc tacccggcca
     1261 ccacgtccac gtcgacctcg gtgggcggca ccgccatcga gcgctggctg cgcccggtcg
     1321 cgtaccagaa caccccggag cccctgctcc cgcccgagct gcgcgacgac aacccgctgg
     1381 ggctgccgcg gcgggtcgac gggaccctgg aacactgacg ccatggacct cgacctcccc
     1441 cagctcccct tctccctgcg cggctacggc cccgacggcc actggtccca cgaggacggg
     1501 gtgctcaccg gctgggcggg cgcccggcag gaccgcttcg tgccgccgac cggcgaggcg
     1561 ctcgaccccg ccgccgacgc gccgcgcctg ctgggcgcgc cggaaggcga cttccagctg
     1621 atcgcccgcg tcacggtcgg cttcgcggcg cccttcgacg cgggcgtgct ctatctgcac
     1681 gtcggcgagc gggagtgggc caagctctgc ctggagcgct ccccggacga gccgacggtc
     1741 tgcaccgtcg tgacgcgcgg ccactccgac gacgccaacg ccttcaccgt ggacggcgcc
     1801 agcgcctggc tgcgcatcag ccgcacgggc tcggcgttcg ccttccacgc gtcggcggac
     1861 ggcgagcggt ggacgttcgt gcggatcttc tcgctcggcg acgaggagtc ggcgggcgcc
     1921 gccctcgtcg gcttcatggc gcagtcgccg gtgggggagg ggtgcgtggt gacgtacgac
     1981 cacatcgagt tccgcccgaa ctggccgaag ggcctgcgcg acggctcgta ggggaatgcg
     2041 ccgcttcgcc cgtgtgttga cggcggtggc ggagggatcc cgcaggggac cctccgcgcc
     2101 gaccgtcacg cacccgcgta ccgcacgacc cggaaagagc cacacatgcg cgtcgagatc
     2161 tggagcgaca tcgcctgccc ctggtgctac gtgggcaagg cccgcttcga gaaggcgctc
     2221 gccgccttcc cgcaccgcga cgccgtggag gtcgtgcacc ggtccttcga gctggacccc
     2281 ggccgcgcca agggcgacac cgggcaggtc cttccgatgc tcatgaacaa gtacggcatg
     2341 accgaggagc aggcccgcga gggcgagcgg cgcctcgggg agaacgccgc cgccgagggc
     2401 ctcgactacc gcatcgaagg ccgcgaccac ggcagcacct tcgacatgca ccgtgtgctg
     2461 cacctcgcca aggagcgggg gcgccaggac gagctgatcg gtctgctcta ccgggcgaac
     2521 ttcgccgagg agcggtccgt cttcgccgac gacgagcggc tcgtcgagct cgccgcagag
     2581 gcgggcctcg acgaggccga ggtgcgcgcc gtcctcgccg acccgggcag gtacgccgac
     2641 gacgtccgcg ccgacgagcg cgaggccgcc gagctgggag cgaacggcgt gccgttcttc
     2701 gtgctcgacc gccggtacgg cgtctccggc gcccagcccg ccgaggtgtt cacccaggcc
     2761 ctcgagcagg cgtggggcgg ccgttccccg ctgaccaccc tcgcggacgc cggtgacggc
     2821 gaggcgtgcg gtcccgacgg atgcgcggtt cccggggcct gaccgggcac cctaggttga
     2881 tcccatgact tccctgggga tcgaccacca cgtcagcgcc gagttcgcgc cggagacgac
     2941 cttcctcaac accgcggtcc gcggcctgct gccgcgccgc gccgccgagg ccgtacaggc
     3001 cctgacggcg gaactcgtct ccggcgggcg gagcggatcg ggcgacttcg ccacggtcga
     3061 ccgggcccgc gcctccttcg cgcgcctcgt cggcgtggac ccctcgcgcg tggcgacggg
     3121 cggctcggtc gccgtgcacg tcggcctcat cgcggcgtcc ctccccgcgg gcgccgaagt
     3181 gctcttcccc gaaggcgact tctcctccct gatcaacccc ttcgtgctgc gcggcgacct
     3241 gaagccgcgg ttcgcgccgc tggacgaact cgccgacgcg atacggccga agaccgcgct
     3301 cgtggcgttc tccaccgtgc agtccgccga cgggcgtacg gcggactgcg cggcgatccg
     3361 cgaggccgcc gccgcccacg gcgcgcgcac cctggccgac accacgcagt cggcgggctg
     3421 gcagccgctc gacgcgggcg cgtacgacta ctccgtgacc ggcgccttca agtggctgct
     3481 ctgcccgcgc ggcacgtcct tcctgacggt cggggaggcg gcgcaggagt ccctggcgcc
     3541 gctgcacggc ggctggctgg ccgcggccga cgcctggaac tcgacctacg gcccgatcgg
     3601 cgaggtcgtc gccgacgccc gccggttcga cgagccgccc gccttcctcg cccaccacgg
     3661 ggccgcggcg gcgctctccc tcatcgagga gatcggcgtc gacgccatcc accgccacga
     3721 cctggccctc gccgcccgct accgcgcggg cctcgccggc ctcggccacg cgcccgtgcc
     3781 gggtgcgtcg cccatcgtct cggtgccggg gctcgccgac cgcgagccgg agctggcccg
     3841 cgcgggcgtc gtcgtcgcgg cgcgggcggg tcacctgcgg gccacgttcc acctctacaa
     3901 caccgaggcg gacgtggacc ggctcctgga cgtgctggcg ggctgaggaa cggaaatcgg
     3961 cccggcgccc acgaggggcg ccgggccgag tcccgggtac gtcaggccgg gcggacccgg
     4021 ccccggtcac ctcaccgggg tgaagtcccg cgcgccgatg aactcgggcc tgcggaccgg
     4081 cgccgcgaac ggctccaccg cggtgttctc cacgctgttg aagacgatga agacgttgga
     4141 ccgcggatag ggggtgatgt tgtcccccga gccgtgcatg gcgttgcagt cgaaccacgt
     4201 ggccgagccg gcctcgcccg tgaagaggcg gatgccgtgg tcggacgcga ggtgggtcag
     4261 cgtctcgttc gacgggatgc ccgcgtcctg catctgcagc gaccgcttgt agttgtcctt
     4321 cggagtggcg cccgcgcagc cgaggaacgt cttgtgcgac cccggcatga tcatgagacc
     4381 gccgttggtg tcgtggttct tcgtcagggc gatcgacacc gacacggtcc gcatgttcgg
     4441 caggccgtcc tccgcgtgcc aggtctcgaa gtcggagtgc cagtagaacc cggaggcccc
     4501 gaagcccggc ttcacgttga tccgtgactg gtgcacgtac acgtcggacc cgaggatctg
     4561 ccgggcccgg cccaccacgc gcgggtcggc ggccagcttg ccgaacagct cactgatctt
     4621 gtgcacctcg aacagggagc ggatctcctg cgaccgcggc tcgacgatgg aacgctcgtc
     4681 ggcgcgcatc gccgggtcgt tgacgagccg ctccagctcg gcgtggtaga cgctcacttc
     4741 gtccggggtg atcagctggt cgatggccag gaaaccgtcc cgctcgaacc ccgccagatc
     4801 cccggccgag atcggccctt cggcacccgg ctcggaccac acgacggggt ccttgcgttc
     4861 gatgagcacc tcttcggcgc ctcgggtcgg gtacaggtct gtggtgcgtt cgggtgcggt
     4921 ggtcatggtg ttgcctgcct ctcctctcgt acggccctgt gcgggatgtc agccgacctc
     4981 agccgacctg cgcgcggctc tcaggcctcg tccggctccg tgagcagcgg gtacacgccg
     5041 ttctcgtcgt ggtcctcgcg acccgtgacg ggcgggttga agacgcagat gcagtggaag
     5101 tcctccttga tccgcatcgt gtgcttctcg tgcccgtcga gcagatacat ggtccccggc
     5161 gtgatcgtgt acttctcacc ggtcgtgtcg tcggtcagct cggcctcgcc cttggtgcag
     5221 acgacggcct cgatgtggtt ggcgtaccac atcgacgtct cggtccccgc gtacaggatg
     5281 gtctcgtgca ccgagaagcc cacccgctcc ttggcgagga cgatgcgctt gctctcccag
     5341 gtgccggacg cggccttcac gtgccggtcg gtgccttcaa tgtccttgaa cgaacggacg
     5401 atcacggtgt atcggttcct tttctcttac ggggtgaggg gctcggtcag ccgccgggtc
     5461 aggcggtctc gcgtaccgcg cgggccagcg tgcgcagccc ctcgtccagc tcttcggggg
     5521 tgatcgtcag cgccggaagc agcttgacga cctcgccctc ggggccggag gtctcgatga
     5581 gcaggcccag ttcgaaggcc cgcttcgcca cggcgctcgc gcggtccttg tccaggaact
     5641 cgatgcccca gaccaggccg cggccgcgga actccttgat gtcggcgcgg ttctcctcga
     5701 cgatcgaggc gagcacctgc tcgacctgct cgccgcgggc gcgggtctgc ttctccatcg
     5761 cggagccgtc ggtccagtag gcctcaaggg cggcggcggc cgtcacgaac gccgggttgt
     5821 tgccgcggaa cgtgccgttg tgctcgcccg gctcccacac gtcgagctcc ggcttgaaca
     5881 ggcagagcga catgggcagg ccgtagccgc tgatggactt cgacaccgtc acgatgtccg
     5941 gcgtgatgcc cgcctcctcg aaggagaaga acgcgccggt gcggccgcag cccatctgga
     6001 tgtcgtcgac gatcaggagc atgtcccagc gctcgcacac atcggcgagc ttgcgcagcc
     6061 actcggcgcg ggcgacgttg atgccgccct cgccctgcac cgtctccacg atcacggcgg
     6121 cgggctggtt gaggccggag ccctggtcct ccaggagccg ctcgaaccag atgaagtccg
     6181 gggtccgccc gtcgaggtag ttgtcgaacg gcatcggcgt gccgtgcacc agcgggatgc
     6241 cggcgcccgc ccgcttgaag gcgttgccgg tcaccgcgag cgaaccgagc gacatgccgt
     6301 ggaaggcgtt cgtgaacgac acgatcgact cgcggccctt gaccttgcgg gccagcttca
     6361 gcgccgactc cacggcgttg gtgcccgtcg ggcccgggaa catgaccttg tagggaaggt
     6421 cgcgcgggcg caggatgatg ttctggaacg actccaggaa ggcgcgcttg gcggtcgtcg
     6481 acatgtcgag cccgtgcgcg atgccgtcgc gctccaggta gtcgatgagc gcgcgtttca
     6541 ggaccgggtt gttgtggccg tagttgagcg agccggctcc ggcgaagaag tcgaggtact
     6601 cgtggccgtc ctcgtcgaac atgcggctgc cctgcgcgcg gtcgaagacc gtaggccaac
     6661 cgcggcagta gctgcgcacc tccgactcca gggtctcgaa gacgctcagg tcgggctggg
     6721 tgatggtcac agcggatctc ctgggagatg gaaagacgtg ggagtggggg gttgcggaca
     6781 tgcgggggga gcgaaaccgg tcagcggccg acgggcccga tgcggtagag cacttctggc
     6841 tcgtgcccgt catcggggaa aagacccgcg tcgaagagga tcttctgctc gaccgccgcg
     6901 ccgtggcgcc gcgcgtagga gaggaacagc cgctcggaag ccgtgttccc cggcgtgatg
     6961 gtcgtctcga cggtgatcgg cgcacgtccg gcggtgacct tcgcggtcag cccgtcgagc
     7021 agggcgccgg ccagaccgcg accgcgctgt gcgtgatcga cggcgacctg ccagaccagc
     7081 agggtgtccg ggcgctcggg ccggaagtag ccggtgatga aaccgaccgg ctccccgtcg
     7141 gcgccccggg ccaccacgga ggtcgccgcg aagtcgcggc accacagcag gtagctgtac
     7201 gaggagttga gatcgagcac cttggagtcg cgggcgatcc gccagatcgc ggcgccgtcg
     7261 gcgacggcgg ggctgtcgat gcgcaacccc tcgggcattt ccaggaattt cgcttgcagg
     7321 tcggtcggta gatcggcttg tgcagcggtc atgcggattg aatttaccga gcaatttctc
     7381 ggattgcatc ggggaaaggg gttacacaag cgcgccccat gtgttatcac gcgggggtgc
     7441 gcgcccgcat gaagacatgg gcttttgtct tgatttgacc gggccatagc ggacaaaacg
     7501 cacgcgatgt ggagtacgtc acaggggtgc gggctctggc gggcgcgtcc ggattgcggt
     7561 gattggcgct gtcgaaatct tagtgtttag gtccccggga agcgggcaga agaatacggg
     7621 aagctgtgct gataaagaat tcatgagaat gcgcggctga attggccgag ggggcgcgga
     7681 gggtgctgta atagaacacc cgattccgat tccggccccg cttctcgggc gtcgcgggcg
     7741 ccgcgggtgc gggcgccgcg ggcgcggacc tcgtgatcgc gctcccgttc ccggccgcgc
     7801 ccgcgcccct gtctccgccc gtgccttcgt ggacgccctc gcacatgtca ggtccccgtc
     7861 acgccgtcga tgcgctccct gatcagatcc gcgtggccgt tgtggcgcgc gtactcctcg
     7921 atgaggtgca ccaggatcca gcgcaggctg accacggcct cgccgccggt gacgaccctc
     7981 tgctcgggac cgagccgccc cgtctcgtcc aggtcgcgcc ccgcgcagat ctcccggctc
     8041 accgcgacct cgcgctccca caccgcgagc acctcggcga acgtccgcga cccgtccacg
     8101 gcgaagccgc cgggcggcat cccgtacagc agcgggacgt cacgcccggc gaacacccgc
     8161 tggatccagt tccgctcgac ctcggccagg tgctgcacca gcccgaacag cgagagcgcg
     8221 gaaggcttca cgcaggcgcg cagcgtctgc tcgtcgtcca gccccgcgca cttcacggcg
     8281 agggtgtcgc ggtgcagatc gagccaaccg gccagcatgg ccggctcgtc ggcggtcagc
     8341 ggcgggatac ggcgtccgtc cggcagcgtg gtcatgcctg gcacgctgcc atgcgggagc
     8401 gcctcggggg aagggggtcc cgaggctctt ctgccttcgc cgcgcgggcg gcgcgccggt
     8461 cagctccagg cgtccgtcac cgcgcggcgg gccccctcca ggtccacggt gacaccggcc
     8521 tcgcccaggg cggcgccgag cgcggccagc gaggcgtgga cgacaccgcg ggtggcgtcg
     8581 ggaccgtagt ggttgacccg gatcatctcc tgggcgaggg cgccgccccc ggcgaccagc
     8641 ggaagcaccg ggtcggcggc gagcgccgcg gcgaccaggt ccgaggcgtc gacgccctcg
     8701 ggcgcgcgca gcgtggtggc gaccggagcc gcgtcggcgg cctcgtgcac gtacggccgc
     8761 agaccgccgc cgagcgcgag cgcgcccgcg cgagtcgcgg cggcggcggt ggcgtgccgc
     8821 gccatgaggg cgtcgaggcc ctccgtctcg atgcgctcca cgcacgcttc gagcgccagc
     8881 atctccagct gcgcgggcgc gtgcagcagg gccttgcgcc cggcgtcgat ccagcgctcc
     8941 ttccagtcga ggagggagag gtaggagcgg cgcggcgccc gcgggttggc ggcgatccgc
     9001 tgccaggccc gctcgctcac cgacaccgcc gagacgccgg ccgggccgcc catcgccttc
     9061 tgggcgccga tcacgcacag gtccacgccc cacgcgtccg ggagcagcgg ctcggcgccc
     9121 accgaggcga ccgcgtccag catgaacagg gccccgtgct cgcgcacgac cgcgccgatc
     9181 tccgcgacgg ggttggtgtt gccggtggcg gcctccgcgt gcaccagcga gacgaagtcg
     9241 atctcggggc gctcggcgag ggcccggcgc acctgctcgg cggtgacggc ggtgtggaac
     9301 ggcacggcga ggtcgacgac gtccgcgccg cagtcccgca gccagttccc gaaggtctgc
     9361 ccgtacggtc ccgtgatcac gttcagcgcg gtcgtgcccg gccgcgccgc ggagcggatg
     9421 cacccctcca ggggcagcag ggcctcgccc tgcatgatca cgacatcctg tccggtgccg
     9481 agcagccggg ccacccgcgc ctcgatggcg gcgaagtgct gggcgctcag cggcgccagg
     9541 tccagaaagg ggtgcgtcac agtactgctc acgtcactgc tcacttcgct cacgagttcg
     9601 tccggctgcg gtgcacgagc ctaccgaccg ccgcatacgg cacgttcccg gcgtcccgtc
     9661 gcacgccttt cccgcacccc gcgcaccgcc tccccggcac cccgtaccgc ccgtcatcgc
     9721 cgcctcctaa gctgctgcca tgagtgatca cgcggtgctg cacgtgaagg ggcgggtgct
     9781 cgtcgggccc gaggacgtac gggacgaact gtgggtggtc ggcggcaggg tgacgtacga
     9841 ccgcccggtg accccggcga cggagacggc caccgtcgag gggtgggccc tgcccggcct
     9901 ggtcgacgcg cactgccacg tgggcctgga cgcgcacggc cccgtggacg ccgccaccgc
     9961 ggagaagcag gcgctgaccg accgcgagat cggcgcgctc ctcatccgcg acgcgggctc
    10021 cccgtcggac acccgctgga tcgacgaccg cgaggacctg ccgaagatca tccgcgccgg
    10081 ccggcacatc gcgcgcacgc gccgctacat ccgcaactac gcccatgaga tcgaacccga
    10141 cgacctcgtc gcatacgtcg cccaggaggc gcggcgcggc gacggctggg tcaagctcgt
    10201 cggcgactgg atcgaccgcg aggcgggcga cctgaccgcc tgctggccgc gcgacgcggt
    10261 cgaggcggcc atcgcggagg cccaccggct cggcgcccgc gtgacggccc actgcttcgc
    10321 ggaggactcg ctgcgggacc tggtggaggc gggcatcgac tgcgtcgagc acgcgacggg
    10381 cctcacggag gacacgatcc cgctc
//
