LOCUS       NZ_CP015588            10531 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces alfalfae strain ACCC40021 chromosome, complete genome.
ACCESSION   NZ_CP015588
VERSION     NZ_CP015588.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces alfalfae
  ORGANISM  Streptomyces alfalfae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8625867)
  AUTHORS   Gu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2016) Institute of Agricultural Resources and
            Regional Planning, Chinese Agricultural Academy of Sciences, 12
            Zhong Guan Cun South Street, Beijing 100081, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04; Celera Assembler v. 8.0;
            GapCloser v. 1.12
            Genome Coverage                   :: 310.8
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:19:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,535
            CDSs (total)                      :: 7,444
            Genes (coding)                    :: 7,210
            CDSs (with protein)               :: 7,210
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 107 of 234
            Pseudo Genes (incomplete)         :: 177 of 234
            Pseudo Genes (internal stop)      :: 26 of 234
            Pseudo Genes (multiple problems)  :: 73 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:53:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4114397
            Orig. end                         :: 4124928
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015588.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10531
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="23"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10531
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10531
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5531](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5531)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(667..1587)
                     /locus_tag="A7J05_RS17455"
                     /old_locus_tag="A7J05_16910"
     CDS             complement(667..1587)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004935403.1"
                     /locus_tag="A7J05_RS17455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_16910"
                     /product="PRC and DUF2382 domain-containing protein"
                     /protein_id="WP_076685200.1"
                     /transl_table=11
                     /translation="MTQGEAFTNPDALKGLTAYDRTGEKIGSIERVYLDDRSGRPEWVT
                     VKTGLFGMKESFVPLSGARLQQDALHVSCTKEAVKEAPRVDADQHLEPGQEHELYAHYG
                     LSRPGMGTTGTGTGTGMGAAGTAAGTGTGMAAGERPMAGAAGGREYAMRGKERDDRAQD
                     MIRSEERLRVGTEEEEVGHAHLRKTVVNEDVTTSVPVTHEEVRVVREPIREGDRSRANI
                     GEAETEVTLHAEHAVVSKESVPVERVHLETERVTETEEVTGTVRKEQIEFDDGTKDSRR
                     RGMKGDRRGTKGTDAWREGKQGPNH"
     gene            complement(1853..3067)
                     /locus_tag="A7J05_RS17460"
                     /old_locus_tag="A7J05_16915"
     CDS             complement(1853..3067)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501751.1"
                     /locus_tag="A7J05_RS17460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_16915"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_076685201.1"
                     /transl_table=11
                     /translation="MKIDCVGGGPASLYLAILAKLKDPAHEVTVHERHAEGASHGWGVT
                     FWPDLLEELRAHDPESAAVIAERCVSWRGGFAHVGDRTTAHDGDLGHAIGRHEMRTILA
                     DRARSLGVRLAFGTEIGGRGDLPDADLVVAGDGAHSRLRTAHAEHFGTRVTTGANRFIW
                     LGTTRVFTAFTFAFVETEHGWIWCYAYGYSGGHSTCVVECSAATWTGLGFDRIAHTDAL
                     RLLEKLFAEPLDGHGLLGRDDIEGHAQWQAFPTVTNATWSHGNLTLIGDAAHTTHYSIG
                     AGTTLALRDAACLAGELRGVGPRELPDALARYERRRKHALLAVQSAARHSAQWYENLPR
                     YIGMPPERMFALLGQRHSPLLPYVPPQIYYHLDQTVGRLNSLRWLKQRLGPRVARALQG
                     RRPES"
     gene            3264..3524
                     /locus_tag="A7J05_RS39455"
     CDS             3264..3524
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A7J05_RS39455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_123985604.1"
                     /transl_table=11
                     /translation="MSAHLTRPLLIFALALVPPLGIVSAAATPTGPSPAVTTTSESSKS
                     ARDASVRDASVHDTSVRDASVGSSPACDWRYGSSCVVGGRM"
     misc_feature    3581..3594
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.23"
                     /tool="antismash"
     gene            3728..4003
                     /locus_tag="A7J05_RS17470"
                     /old_locus_tag="A7J05_16925"
     CDS             3728..4003
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116479.1"
                     /locus_tag="A7J05_RS17470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_16925"
                     /product="chaplin"
                     /protein_id="WP_062774722.1"
                     /transl_table=11
                     /translation="MSRIAKVTAATLGTSAVVLGGAGMAMADADAKGAAVGSPGAISGN
                     VVQVPVHAPINLCGNTVNVVGLLNPAFGNECTNVSKHEHGHGGYGR"
     gene            complement(4091..4963)
                     /locus_tag="A7J05_RS17475"
                     /old_locus_tag="A7J05_16930"
     CDS             complement(4091..4963)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116480.1"
                     /locus_tag="A7J05_RS17475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_16930"
                     /product="tyrosinase family protein"
                     /protein_id="WP_076685203.1"
                     /transl_table=11
                     /translation="MAPTRKNQSRLTKAERRRFVDAVLKLKRRGEYDAFVRTHLDFYVS
                     DGESRLRVAHMAPSFFPWHRRFLLDFERALQRIDAGVTVPYWDWTKDRTPAASLWGDDL
                     MGGTGRGPDRRVTTGPFARGSGHWVIKVGVTDGDFLTRDLGRPRAPIALPTAREVADAM
                     ADPRYDVPPYDSTARAGFRNRLEGWTSARGDARWRNHNRVHRWVGGHMLGGASVNDPVF
                     WLNHAFVDLLWDRWRRRHPRSAPYLPAEPPGAGSSQYRRVVARHERMPPWDVRPDDLLD
                     HRAHYRYGG"
     gene            complement(5001..5531)
                     /locus_tag="A7J05_RS41670"
                     /old_locus_tag="A7J05_16935"
     CDS             complement(5001..5531)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017995.4"
                     /locus_tag="A7J05_RS41670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_16935"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_335755779.1"
                     /sec_met_domain="melC (E-value: 1.4e-40, bitscore: 137.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGPARPAHERHRHARPHALVGLLDERNGGVSRPWGTHGEPERL
                     VDRVTRRHLLGVAASALLAAAEIMRTPWVTREQSPAPAQAPAEVPDGPPAGFDEMYRGR
                     RIQGEPVAVREGRTPESWRITVDGRPLGLMRRADGSFLSMVDHYQSYDTPLAAARGAVD
                     EIGPLQTLSGRHH"
     gene            complement(5435..6097)
                     /locus_tag="A7J05_RS17485"
                     /old_locus_tag="A7J05_16940"
     CDS             complement(5435..6097)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473218.1"
                     /locus_tag="A7J05_RS17485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_16940"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_076685205.1"
                     /transl_table=11
                     /translation="MSATELPLRLPAQSAGGRGPAPAPPAEGPGRWFGVLLVVTGAAGL
                     LAAWAITLDKFKLLEDPDFRPGCSLNPVVACGSIMRSEQAAVFGFPNPLLGLVAYAVVA
                     GVGASVLAGARFPRWYWLALNAGMLFGVAFCTWLQFQSLYRVNALCLWCCLAWAATILM
                     FWSVTAHNVRRGFLPAPRGLRTVVAECAWVPPALHMSVIGMLVLTRWWDFWTSGTAG"
     gene            complement(6401..6679)
                     /locus_tag="A7J05_RS17490"
                     /old_locus_tag="A7J05_16945"
     CDS             complement(6401..6679)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644659.1"
                     /locus_tag="A7J05_RS17490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_16945"
                     /product="hypothetical protein"
                     /protein_id="WP_062774713.1"
                     /transl_table=11
                     /translation="MKAMKAAAVLAGSLAIAGSAAVPAFAGAQAPSGPNGGLDDLASKG
                     LEAATPVGTNMLNPKNSDSVANTVKGVVRGLSSEGPGKLIGGLPAGK"
     gene            complement(6868..7446)
                     /locus_tag="A7J05_RS17495"
                     /old_locus_tag="A7J05_16950"
     CDS             complement(6868..7446)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A7J05_RS17495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A7J05_16950"
                     /product="hypothetical protein"
                     /protein_id="WP_062774710.1"
                     /transl_table=11
                     /translation="MRALPVRRIATTTLCATLLLGTAGPALASADSASREETRTAPRAP
                     VMTPKAMLAEAKRLQDAGGVITPAADLLVAVLEAEGYRLSAAEAAKHAQAVRTEARKVS
                     ARQPAAPAAAPAARAPEPPAGSLPALQKTVDNMLKATTTGDATGVVREGRSLVTGLLQG
                     VLGGLGGAAQPAPAPAAAPAAGPQQTPAT"
     gene            complement(7707..7946)
                     /locus_tag="A7J05_RS17500"
                     /old_locus_tag="A7J05_16955"
     CDS             complement(7707..7946)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="A7J05_RS17500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_16955"
                     /product="chaplin"
                     /protein_id="WP_062774708.1"
                     /transl_table=11
                     /translation="MSRTTKVLAFSAVAAAAVAGSAGAASADADAKGTASNSPGVVSGN
                     VVQVPVNVPVNVCGNTVNIVGLLNPAFGNKCVNH"
     misc_feature    8237..8251
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.14"
                     /tool="antismash"
     misc_feature    complement(8293..8308)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.24"
                     /tool="antismash"
     gene            8333..9508
                     /locus_tag="A7J05_RS17505"
                     /old_locus_tag="A7J05_16960"
     CDS             8333..9508
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644663.1"
                     /locus_tag="A7J05_RS17505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_16960"
                     /product="glycosyltransferase"
                     /protein_id="WP_076685206.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 7.3e-34,
                     bitscore: 115.6, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRHSPGPRILHVSRPVEGGVAQVVTDLARAQAATGADVHVACPDE
                     GMLPRVWDTADGGVRGHPWTASRSPGPQLAAEVRDLARVIARVRPALVHAHSAKAGLAA
                     RLALRGRVPTVFQPHAWSFEAVGGVTAPLTRCWERRAARWTDRIVCVSEAEWITGRRAG
                     VTGAYTVIPNGVDVARFAPGRRSEARRRLLPDSFARAPLVVCVGRLCRQKGQDVLLRAW
                     AAVAEALPGARLVLVGDGPDAAGLRDLADTTVTFAGATDDTACWYRAADVVVLPSRWEG
                     MALAPLEAMACGRPVVVTDVDGARESLPPADRAHALVPPSDPATLARALLALLRDGPLR
                     AELGRRGRRHVLDHHDARRTASAIDAVYRELLGPAPTTYPATAKYRECTTT"
ORIGIN
        1 gcactttggc cgccggcggt gcggggatgg ccggtgggac cctcctgctc accggcatgg
       61 tcgccgcccc ggtggcagcg ctggccgccg caggtttcta cgtcgtgagg cagcgtcgga
      121 acaagaagga ggaggagcgt ctccgaaccg aggtcgaagc cgcggaggcg gctctggacc
      181 agtcgcagcg gggcttcgac gcgatgatcg acgtactgca tcgcgccacg gagatcatgg
      241 agtacgtcag cgtccacggc acccacgccc tcgagaagtg gagggtgaat ctgccgccgg
      301 agccccggca ctgggcatcg ctcgggcatg aggggcagga gcggtaccgg gagttcctga
      361 ccgtggccgg ttgcctcctc gctgtgagca gcatcaacgt cagcgccctc ctgacggcac
      421 ggcccgacgc cgtgcgtgag atgggcagga ccatcgacga gacgctgcgg tacgcggaca
      481 ggaccatcaa gtcgatcgtc tgagcccgat ggggcgtggt catcggccgc tcatcggaca
      541 cgacgcgtgg cgcggcgagg cagcaggggc aggcggcggc gcggcccggc cccggtcgga
      601 catggcgaag gccctcaccc ggtgggtgag ggcctctgcc ggggggccgg tggccctggg
      661 cgcgggtcag tggttcgggc cctgcttgcc ctcgcgccag gcgtcggtgc ccttcgtgcc
      721 ccggcggtcg cccttcatgc cgcggcggcg gctgtccttc gtgccgtcgt cgaactcgat
      781 ctgctccttg cggacggtgc cggtgacctc ctcggtctcc gtgacccgct cggtctccag
      841 gtggacgcgc tccaccggca cggactcctt gctcaccacc gcgtgctcgg cgtggagggt
      901 cacctccgtc tccgcctcgc cgatgttggc gcgcgagcgg tcgccctcgc ggatcggctc
      961 gcggacgacg cggacctcct cgtgcgtgac cggcacggac gtcgtgacgt cctcgttcac
     1021 caccgtcttg cggaggtggg cgtggccgac ctcttcctcc tcggtgccga cccgcagccg
     1081 ctcctcggag cggatcatgt cctgcgccct gtcgtcgcgc tccttgccgc gcatggcgta
     1141 ctcgcggcca ccggcggctc cggccatcgg gcgctcgccc gcggccatgc cggtgcccgt
     1201 accggcggcc gtaccggcgg cgcccatgcc cgtgcccgta ccggtgccag tggtgcccat
     1261 gccggggcgg gacaggccgt agtgcgcgta cagctcgtgc tcctgaccgg gctccaggtg
     1321 ctggtcggcg tcgactcgcg gcgcctcctt cacggcctcc ttggtgcacg agacgtgcag
     1381 ggcgtcctgc tggaggcgcg ccccggacag cggcacgaag ctctccttca taccgaagag
     1441 gcccgtcttg acggtcaccc actcggggcg gccgctgcgg tcgtcgaggt agacccgttc
     1501 gatgctgcct atcttctcgc cggtccggtc ataggctgtc aggcccttga gcgcgtcagg
     1561 gttggtgaag gcctcgccct gcgtcatggg tgatcactcc ctttgagcat gcgacgggcg
     1621 aaatccgatc cgccacggaa cgacgcacgc ctcttaaggt ccatcgcgcg ccgccccggc
     1681 ccgccccgca aggcggggag tcacggagcg tgcggggaac ggctcgcccg cgttctccgc
     1741 cccggcaacc gaggcgatcg agccggtcac agcggtttcg gccccgaggg tccgaacggg
     1801 tgaacacttg gggtttgcat ccatgggcca ccggccgtcg ggactccgcc cgtcaggact
     1861 ccggccgccg gccctgcagc gccctggcca cgcggggccc cagccgctgc ttcagccagc
     1921 ggagggagtt gagtcggccg accgtctggt ccaggtggta gtagatctgc ggcggcacat
     1981 agggcagcag cggcgagtgg cgctggccga ggagcgcgaa catccgctcc gggggcatgc
     2041 cgatgtagcg gggcaggttc tcgtaccact gggcgctgtg ccgggccgcg ctctgcaccg
     2101 cgagcagggc gtgcttgcgg cggcgctcgt agcgcgcgag ggcgtcgggc agctccctcg
     2161 ggcccacgcc cctcagctcc cccgccaggc acgcggcgtc gcggagcgcc agcgtggtgc
     2221 ccgcgccgat ggagtagtgc gtggtgtggg cggcgtcgcc gatcagcgtc aggttgccgt
     2281 gggaccaggt ggcgttggtg accgtcggga acgcctgcca ctgcgcgtgg ccctcgatgt
     2341 cgtcgcggcc gagcagcccg tggccgtcca gtggctcggc gaacagcttc tccagcagcc
     2401 gcagggcgtc ggtgtgggcg atccggtcga agcccaggcc ggtccaggtg gccgccgagc
     2461 actccaccac gcaggtgctg tgcccgccgc tgtacccgta cgcgtaacac cagatccagc
     2521 cgtgttcggt ctccacgaac gcgaaggtga aggccgtgaa gacgcgggtc gtgccgagcc
     2581 agatgaagcg gttggcgccg gtggtcaccc gggtgccgaa gtgctccgcg tgcgccgtgc
     2641 ggagcctgct gtgcgcgccg tcgcccgcga cgaccaggtc cgcgtccggc aggtcgccgc
     2701 gaccgccgat ctcggtgccg aacgccaggc gcacgccgag ggagcgggcc cggtccgcga
     2761 ggatcgtccg catctcgtgg cgtccgatgg catggcccag atcgccgtcg tgcgcggtgg
     2821 tcctgtcgcc gacgtgggcg aagccgccgc gccagctcac gcaccgctcg gcgatgaccg
     2881 cggccgactc ggggtcgtgc gcccgcagct cctccaggag gtccggccag aaggtcacgc
     2941 cccagccgtg gcttgcgccc tcggcgtgcc gctcatggac ggtgacctcg tgggccgggt
     3001 ccttgagctt cgccaggatg gccagataca gggatgcggg gcctccgccg acgcaatcga
     3061 tcttcacggg gactcctgtc gccgagggaa gcggaacggt gacataaagt agccgcccgt
     3121 ggcgtctccg ccgcccaggg cgcggcagtg tcgccgcacg tcaccccacc gccgctcacc
     3181 gaaagtgatc ggcatgcgat cttgggtgag atgtgtgcac catcactctg tctcgggtta
     3241 cgtcgatcac gaggagaccg gacgtgtcgg ctcacctcac caggccgctc ctgatcttcg
     3301 cgctggccct ggtccccccg ctggggatcg tgtccgcggc ggccacgccg acggggccct
     3361 ccccggcggt gaccaccacc tccgagagca gcaagtccgc aagggacgcc tccgtacggg
     3421 atgcctccgt ccacgacacc tctgtgcggg acgcctccgt cgggagttcg cccgcctgcg
     3481 actggcggta cggcagctcg tgcgtcgtcg gcgggcgcat gtgacaccgc gcggggcgag
     3541 cggcgccgaa ccaccacgcg acgtccctct cctccacgcg accactctgc gtgccatctc
     3601 atctgcgaaa tgtccttgtt catggcggtc gcggcactcc ttgccgtgac ttcacgttga
     3661 cgcggggcgt taacgagggt gcggctgcgc gtaggcagcc gcgctcacca agtcaaagga
     3721 gaacgtgatg tctcgcatcg cgaaggtcac cgccgccacc ctcggtacca gtgccgtcgt
     3781 actcggtggc gcgggcatgg ccatggcgga cgcggacgcg aagggcgctg ccgtcggttc
     3841 ccccggcgcc atctcgggca acgtcgtgca ggttccggtc cacgccccga tcaacctctg
     3901 cggcaacacc gtcaacgtcg tcggcctgct gaaccccgcc ttcggcaacg agtgcacgaa
     3961 cgtcagcaag cacgagcacg gccacggcgg ctacggccgc tgagtgcccc cgggccgccc
     4021 cgagcggccc gccggcccgc cggccctcgg tgagcggtcc accggccccc ggtgaacggc
     4081 ccgccggggg ctaccccccg taccggtagt gcgcgcggtg gtcgaggagg tcgtccgggc
     4141 gcacgtccca cggcggcatc cgctcgtggc gcgcgacgac gcggcggtac tgggacgagc
     4201 cggcgcccgg cggctccgcg ggcaggtagg gggccgagcg gggatggcgg cggcgccagc
     4261 ggtcccacag caggtcgacg aaggcgtggt tcagccagaa gaccgggtcg ttgacggagg
     4321 ccccgccgag catgtgcccg ccgacccagc ggtggacccg gttgtggttg cgccaccgcg
     4381 cgtcgccccg cgccgacgtc cagccctcca gccggttgcg gaaacccgcg cgtgccgtgg
     4441 agtcgtaggg cgggacgtcg tagcgcgggt ccgccatcgc gtcggccacc tcccgcgcgg
     4501 tcggcagggc gatgggggcg cgcggacgcc ccaggtcgcg agtgaggaag tccccgtcgg
     4561 tgacgcccac cttgatgacc cagtggccgc tcccccgggc gaacggcccc gtcgtgaccc
     4621 ggcggtccgg cccccgtccc gtgccgccca tcaggtcgtc gccccacagg gacgccgccg
     4681 gggtccggtc cttcgtccag tcccagtacg ggacggtcac gcccgcgtcg atccgctgga
     4741 gcgcccgctc gaagtccagc aggaagcggc ggtgccacgg gaagaacgag ggggccatgt
     4801 gcgcgacccg caggcggctc tcgccgtccg agacgtagaa gtcgaggtgc gtgcgcacga
     4861 aggcgtcgta ctcaccccgg cgcttcagct tcagcaccgc gtcgacgaac ctgcgccgct
     4921 cggccttggt cagcctgctc tggttcttgc gcgtgggggc catgtccgtc cgttccgcgt
     4981 tccggggtgg gccgccggac tcagtggtgc cggccgctca gggtttgcag ggggccgatc
     5041 tcgtccacgg cgcctcgtgc ggcggccagc ggggtgtcgt acgactggta gtggtcgacc
     5101 atgctgagga agctgccgtc ggcgcggcgc atgaggccca gcggacggcc gtccacggtg
     5161 atccgccagc tctccggcgt acgtccctcg cgcacggcca ccggctcccc ctggatgcgg
     5221 cggccccggt acatctcgtc gaagccggcg ggcgggccgt ccggcacctc ggccggggcc
     5281 tgcgccgggg ccggactctg ttcgcgcgtg acccagggcg tccgcatgat ctcggcggcg
     5341 gcgagcaggg ccgaggccgc gacgccgagg aggtgacggc gcgtcacccg gtcgacgagc
     5401 cgttccggct caccgtgcgt gccccacggc cggctcaccc cgccgttccg ctcgtccaga
     5461 agtcccacca gcgcgtgagg acgagcatgc cgatgacgct catgtgcagg gcgggcggga
     5521 cccaggcgca ttccgcgacg acggtacgca gcccccgggg cgcggggagg aatccgcgcc
     5581 ggacgttgtg cgccgtcacg gaccagaaca tgaggatggt cgcggcccag gccaggcagc
     5641 accacaggca gagcgcgttg acgcggtaga gcgactggaa ctggagccag gtgcagaagg
     5701 cgacgccgaa gagcatgccc gcgttgagcg ccagccagta ccagcggggg aagcgggcgc
     5761 ccgccagcac gctcgcgccc acgccggcca cgacggcgta ggcgacgagg ccgaggagcg
     5821 ggttggggaa cccgaagacc gccgcctgct cgctccgcat gatgctgccg caggccacga
     5881 ccgggttgag gctgcacccc ggccggaagt ccgggtcttc gagcagcttg aacttgtcga
     5941 gcgtgatggc ccacgccgcg agcagccccg ccgccccggt gaccacgagc agcacgccga
     6001 accaccggcc gggcccctcg gcgggcggcg ccggggccgg gccgcgcccg cccgcggact
     6061 gcgccggcag ccggagcggc agttccgtgg cgctcatcgg ccggggccgg gcgtcggcgg
     6121 cgggcggtgc gcggcgtcgc gggggccgcc ggaagtcgtg gtcgtggtca tgggggagac
     6181 cgtatagacg cccggcggcg cgaccggctc cgttgtcacc cgggtggcgg agccgcgggc
     6241 agccatcaca cgatggggtc acaccggtgg ggctcaggac ggctccgggt cgaactggtg
     6301 gccgtacggg acgccttgac cgcccgtgcg cacgccggag ggccggcccg tcgaagggag
     6361 ccggccctct gtgttcccgg gggggctgcg cgaggcgcgg ctacttgccg gcggggaggc
     6421 cgccgatgag cttgccgggg ccttcggagc tcaggcccct gacgacgccc ttgaccgtgt
     6481 tggccacgga gtcgctgttc ttggggttga gcatgttcgt gcccaccggg gtggcggcct
     6541 ccaggccctt gctcgcgagg tcgtcgaggc cgccgttcgg gccggagggc gcctgggcgc
     6601 cggcgaacgc gggcacggcg gcggaacccg cgatggcgag ggaaccggca aggacggcgg
     6661 cagccttcat ggccttcatc ttgttccttt ctctggcaac gcgtgtcgcc cggtggacgt
     6721 gccgccctgg cgtcgatcgc ccggacgtgt cacttcacct gtcgtgtcac ttcgtaagga
     6781 accaacgagc acgggccggt acggaaaccc ctggaccggg aacttcccgc ggacccgcgc
     6841 gcatgaaggc cgcccgccgt ccgccgctca ggtcgcgggc gtctgctgcg gtccggcggc
     6901 cggggccgca gcgggcgccg gggccggctg ggccgcgccg ccgaggccgc cgagcacgcc
     6961 ctggagcagc cccgtgacga gcgagcgccc ctccctgacg acgccggtgg cgtcaccggt
     7021 cgtggtggcc ttgagcatgt tgtcgaccgt cttctgcagg gcggggaggc tgcccgccgg
     7081 gggctcgggg gccctggcgg ccggggcggc ggccggagcg gcgggctgcc tggcggacac
     7141 cttccgggcc tccgtccgta cggcctgggc gtgcttcgcg gcctccgcgg cggagagcct
     7201 gtacccctcg gcctccagca ccgccaccag caggtcggcg gccggggtga tgacaccgcc
     7261 ggcgtcctgc agccgcttcg cctcggccag catggccttc ggcgtcatga cgggtgcgcg
     7321 gggcgcggtc ctggtctcct cgcgggaagc ggagtccgcg gaggcgagcg cggggccggc
     7381 cgtgccgagg agcagcgtcg cgcacagggt ggtggtcgcg atacgccgta cgggcagagc
     7441 gcgcataggt cacagccttt cggtgatacg tcggatcttg cgatcaccgt gcgagcggtg
     7501 cgggcccacc gcaaccgagc ggtccacccg gcccggcgct ccaccgggcg tccgggagca
     7561 tcagcgcagg tggggacgag atttgacgga cggtcggata atttcctgac gccactccat
     7621 tcggggcacg ggcccccggc gactgctccg aacgcgggaa cggccgcccc cgcctccgcg
     7681 gggacggccg ttctccgggc ggtgtgtcag tggttgacgc acttgttgcc gaacgcgggg
     7741 ttcagcaggc cgacgatgtt cacggtgttg ccgcagacgt tcacggggac gttgaccggc
     7801 acctgtacga cgttgccgga gaccacgccg ggtgagttgg aggcggtgcc cttcgcgtcg
     7861 gcgtccgcgg aggcggcacc cgccgatccg gccacagcgg cggcggcgac ggcggagaag
     7921 gcgaggacct tggtggtacg ggacatgggg tgctgctcct tggcaaaggt gtgacgcacg
     7981 ggcggaatgc ccgtgtacgg gaaatcaact gccgatccgg acgccgagtt gtgcacccag
     8041 aagagtgatg tgtgccgtgc gccccgctga cggcgagggc accggccgac gccgcgaccg
     8101 gcggcccgct gggcgcaccg gatacgtgtg ccgttcccat cgaccccgcc ccaccacgca
     8161 tttcaccgat ccgacacacg cgccgggatc ggcggagcta ctcctccacc gagctacagt
     8221 tgcgcatcgc attgggatat ttcagtcata tacgtaagat cccggcattc cttgcagatg
     8281 ccttatctcc gctgtggttc agagctcttc acgtcggaag gccaacgccg tcatgcgtca
     8341 ctccccgggc ccgcggatcc tgcacgtcag ccggcccgtc gagggcggtg tggcccaggt
     8401 ggtcaccgac ctggcccgcg cccaggccgc gacgggcgcc gacgtccacg tggcctgccc
     8461 cgacgagggc atgctgcccc gggtctggga caccgccgac ggcggcgtac gcggccatcc
     8521 gtggacggcg tcgcgctccc ccggcccaca actcgccgca gaggtacggg acctggcccg
     8581 cgtcatcgcc cgcgtgcggc cggcgctcgt gcacgcccac agcgccaagg cgggcctcgc
     8641 cgcgcgcctc gccctgcgcg ggcgcgtccc gaccgtcttc cagccgcacg cctggtcctt
     8701 cgaggcggtc ggcggcgtca ccgcgcccct gacccgctgc tgggagcggc gcgccgcccg
     8761 ctggacggac cgcatcgtct gcgtcagcga ggccgagtgg atcaccggcc gcagggcggg
     8821 cgtcacgggt gcgtacacgg tgatccccaa cggcgtcgat gtcgcccgct tcgcccccgg
     8881 ccgccggtcc gaggcccgtc gccggctgct gcccgactcc ttcgcgcggg cccccctcgt
     8941 ggtgtgcgtg ggccggctgt gccggcagaa ggggcaggac gtcctgctcc gcgcctgggc
     9001 cgccgtcgcc gaagcgctgc ccggggcccg gctcgtcctc gtcggcgacg gccccgacgc
     9061 cgccgggctg cgggacctgg cggacacgac cgtgacgttc gcgggggcga cggacgacac
     9121 cgcctgctgg taccgggcgg ccgacgtggt cgtcctgccc tcccgctggg agggcatggc
     9181 cctggccccg ctggaggcca tggcctgcgg ccgtcccgtg gtggtcaccg acgtggacgg
     9241 agccagggag agccttcccc cggccgaccg cgcgcacgcc ctcgtaccgc ccagcgaccc
     9301 ggcgacgctg gcacgggcgc tgctcgccct gctgcgggac ggcccgctgc gggccgaact
     9361 cgggcggcgg ggccgccgcc atgtcctcga tcaccacgac gcccggcgga ccgcgtcggc
     9421 gatcgacgcg gtgtaccggg agctgctggg cccggcgccc accacgtacc cggcgaccgc
     9481 caagtacagg gagtgcacga ccacgtgagt gccgaacgaa ccgtcgcctc ctccgcggga
     9541 cagccgcggc aggacacgta ccccccggcc ggacccctcg tcgtggcgcc ccgcggcgcc
     9601 ctctgcccgc gcgccggcgc cccggcgagg gggcccgcgc gggggcggcg cacggccgcc
     9661 gccctggtca ccgtggacgc cgccgccgcg ctgctcggca tgttcctgct ggccgatccc
     9721 cgccgcatcg ccgtgctcgc ccccctgctc gtcgtcggcg tcctcgccct gaacgcccgg
     9781 ggccgcctgt acgcgtacac cgccgtgccc gccacgctgg acgaggtgcc ctcgctctgc
     9841 ggccgggtcg cgctcggctg ggccctcgtc gcggcgctcg cgcccgccct gcccgcgcac
     9901 atgccgcagc tcgccccacg cacgctgctc atgatggccg ccacgcagtg cctgatcggc
     9961 gtctgcggac gcgccctggt ccaccggtgg ctgcgggccc gcgccctgcg aagaccacgg
    10021 ccggtcctgg tgctcggctc cggcgggcag gcccaggccg tggccgcggc cctgctgcgg
    10081 cggccccgct gcgggctgcg ccccgtcggc gtcgtggacg aggcggcgga gcagcggccc
    10141 gccgacgagg acggcgccga gccgtccgcc gcgcccggac tgccggtgct gcgcacggag
    10201 gacgaactgc accgggccgt catccagaac gacgtgcgcg ccgtcctgct gctcgcgggc
    10261 cacggcggac ccgccgaccg gctgtccgcc ctgcgcggcc tcggctgcga cctctgggag
    10321 atcgacccgc gcggccccgg cctcgcctcg cccggggaga gcgcccgcgc ctacgtggcg
    10381 gggttccgct gccggccgct gctgcccgcc gggggccggg ggaccggcct cggcaagcgg
    10441 ctcctcgact tcgcggtctc cggcgtcctg ctgctcctcg ccgcgcccgc gctgctcgcc
    10501 tgcgcgctga tcctgcggac cacggagggg c
//
