LOCUS       NZ_CP015588            11284 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces alfalfae strain ACCC40021 chromosome, complete genome.
ACCESSION   NZ_CP015588
VERSION     NZ_CP015588.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces alfalfae
  ORGANISM  Streptomyces alfalfae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8625867)
  AUTHORS   Gu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2016) Institute of Agricultural Resources and
            Regional Planning, Chinese Agricultural Academy of Sciences, 12
            Zhong Guan Cun South Street, Beijing 100081, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04; Celera Assembler v. 8.0;
            GapCloser v. 1.12
            Genome Coverage                   :: 310.8
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:19:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,535
            CDSs (total)                      :: 7,444
            Genes (coding)                    :: 7,210
            CDSs (with protein)               :: 7,210
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 107 of 234
            Pseudo Genes (incomplete)         :: 177 of 234
            Pseudo Genes (internal stop)      :: 26 of 234
            Pseudo Genes (multiple problems)  :: 73 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:53:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2193214
            Orig. end                         :: 2204498
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015588.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11284
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="15"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11284
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11284
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6284](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6284
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            78..293
                     /locus_tag="A7J05_RS09170"
                     /old_locus_tag="A7J05_08785"
     CDS             78..293
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983655.1"
                     /locus_tag="A7J05_RS09170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08785"
                     /product="hypothetical protein"
                     /protein_id="WP_062775595.1"
                     /transl_table=11
                     /translation="MADDESYCAAPGTDTAAAVRALGLGPDAAAEGPFAACVLCQEPTE
                     YPESHKGATLCPVCAWQEAERQACSG"
     gene            complement(301..1545)
                     /locus_tag="A7J05_RS09175"
                     /old_locus_tag="A7J05_08790"
     CDS             complement(301..1545)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A7J05_RS09175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A7J05_08790"
                     /product="hypothetical protein"
                     /protein_id="WP_123985590.1"
                     /transl_table=11
                     /translation="MRGADGTSGVGRAMGVRRPRAAACAVLAVLLTASGCGNGSEPDGS
                     RTGGSGASRPESASPRWEFEHIADFSGGFTDVAALGEDDIWAVGTENNGASNAHLLHYD
                     GERWKREPLPEALGDTDYPPVLEEIGDDTLWLRPQKYATGDGVTPWVRWDGVRWSAVPN
                     PPPGNVGDLEAAGPRDIWALTGERTAVHFDGSRWTTTRLPHRTVDLAVAGPDDVWAVGS
                     RSTGPGTEQVHGEPYDQPASSHWDGTSWQQVETPQSRFDEPLPPEPGAGLLKVFALADG
                     EVRAYGNNTFNHGEVEDEPQEEFIRLRRADDRWVEQEEAPGGCALRVPVAQDDEGLFLD
                     GNWFLHDDGRCLKIKRHRLPVSTGARKGSGQTLWLEEIHRVPGTDTWLGAGKVQVTQSG
                     DPFGAPVLVRLKRAA"
     gene            complement(1663..2331)
                     /locus_tag="A7J05_RS09180"
                     /old_locus_tag="A7J05_08795"
     CDS             complement(1663..2331)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="A7J05_RS09180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08795"
                     /product="ABC transporter"
                     /protein_id="WP_076684014.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLARSQRWLAPVILYAVFLGVGVQSGQPVLDSLAY
                     AAAVLLPVAAWLVRICAGSEPPAARACTAAAAGPARAHLAALLTAFLAAVALGVTAVLV
                     VTAVSDAASTNARLSVPRLPAGAAGLLAMLVSALLGTAVGALTSRPLLRSAGKAVPAML
                     LGALLLLVTTGSPAKSVLTDLTTGSRDGTVPVPWGAVLAALAVAVAAFWSACALSSRRG
                     "
     gene            complement(2328..3245)
                     /locus_tag="A7J05_RS09185"
                     /old_locus_tag="A7J05_08800"
     CDS             complement(2328..3245)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602442.1"
                     /locus_tag="A7J05_RS09185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08800"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_076688779.1"
                     /transl_table=11
                     /translation="MERDPVLRLSGVGRRYGLRGPWVLRGVDLGIRAGALIRVQGGNGT
                     GKSTLLRLLAGVDAPSEGRLTQRPRTAFVPERFPAALPFTAVGYLTHLARVHGLARGAA
                     GRSAERWLERFGAGGHARTPLSELSKGGSQKVAVAQALLADPELLVLDEAWTGLDVPAR
                     AELDRAVAERTAAGGSVVFVDHDPRRLADVADETYAVVGGGLERRTEPPGPEGAGPRGP
                     VVVIVAQGPPGAALPAPVAAVAVGVGAGGAAGATRLTVPESRSDLVLRELLTARVPWHV
                     VRVEEPGAVPSPTSAPAAEEGTSR"
     gene            3313..4068
                     /locus_tag="A7J05_RS09190"
                     /old_locus_tag="A7J05_08805"
     CDS             3313..4068
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128420.1"
                     /locus_tag="A7J05_RS09190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08805"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_076688781.1"
                     /transl_table=11
                     /translation="MSSDETTSTGTRDGGPAADSPFRHESTPRDEAPQFVLPLVARIER
                     AAPPARTDALETAARAVLVMLSDERSLGDGEWARAMRDWQDARIRKVVRRARGGEWRKA
                     ETLPGITVTGKSAEVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPPGGADATHPVLWMS
                     PDVDMSAGKAMAQAGHGAQLAWWELSAEDRTAWRDAGFPLSVRTAAPAQWRALTTSGLP
                     VVRDAGFTEIAPGSCTVVADHPALRTPRH"
     gene            4126..4896
                     /locus_tag="A7J05_RS09195"
                     /old_locus_tag="A7J05_08810"
     CDS             4126..4896
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643870.1"
                     /locus_tag="A7J05_RS09195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08810"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_076684015.1"
                     /transl_table=11
                     /translation="MRHVNGTALIIAALVATLGAIAFPVWSYADRSGTGEADLAAGTVP
                     TRWGPLSAADRDLLVKVRLAGLWEIPAGQQAVERAPTEAVKEAGDHLIVGHTDLDKRVR
                     SAAAQLGVELPNQPTAQQQGWLDELTEASGETYQRTFANLLRASHGKVFSVIAQVRDTT
                     RNSLVRQLATDSNQTVLDHITMLEATGLVDFDDIANGAAADATESPTGPPPPPSGAAPP
                     EAPPAGPEGEVSTTSRPSPGPPGRIDTGRPEPQM"
     gene            5001..6284
                     /locus_tag="A7J05_RS09200"
                     /old_locus_tag="A7J05_08815"
     CDS             5001..6284
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073955.1"
                     /locus_tag="A7J05_RS09200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08815"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_076684016.1"
                     /sec_met_domain="DUF692 (E-value: 2.5e-98, bitscore: 327.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIADAVEAMPGIDWVEAVAENICPGHLPDSLLR
                     LRERGVTVVPHGVSLGLGGADRPDEGRLTALAERAEALGSPLVTEHIAFVRAGGALTAT
                     QPLEAGHLLPVPRTRDALDVLCENVRAAQDALPVPLAVENIAALIAWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPVKALEELPVEAIAYVHVAGGFERDGVWHDSH
                     AHPVPEPVLAILADLASRTAPPGVLLERDENFPGPAELERELDAIRATVKTSPAEPGAA
                     PDTVPPASAAARQRTALAQAALLSALVAGTPAPEGFDHTRLKVQSRALGAKRADVVAKV
                     APELPEILGAAYRAEFLGYARVRPMTGGYRHDALGFAEHLLLAGRPASPDARQRLSDWW
                     LDRSGPAPRSRRPAARLLHATRRVLLRR"
     gene            6289..7341
                     /locus_tag="A7J05_RS09205"
                     /old_locus_tag="A7J05_08820"
     CDS             6289..7341
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049031.1"
                     /locus_tag="A7J05_RS09205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08820"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_076684017.1"
                     /transl_table=11
                     /translation="MSVAAVVYATAVLLSCVPLVMGVVTVRRGGSRHGEGSRAGDGAFV
                     RGTPEAAFLSGGPARAADAVIAGLHEDGRLVVAGPGVVGITRPVARNAAEQAVIDAHTA
                     APSGALHWLRAAVMRSRPVQETGDALAERGLVVRPGPRRTWRRWAGAQVFLTFIGLPTA
                     GILTALQYADDDYTEPDRLLDSVLHVLLPAAALGIGVGVVSLTLTSGQLTPAGRRALHQ
                     YVDSSRHLTGAAHLVATRGLPAAHPDLRSQLIAAARARGLAAPPSSPAPLPFQDPGLWS
                     AGAAAWCGSTTGFGGAGGGSACSGGGGGAACSGSGSACSGGSSCSGGGSSCGGGGGGSS
                     CGGGGGGGSC"
     misc_feature    complement(6301..6312)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: weak, score: 17.94"
                     /tool="antismash"
     gene            7488..8231
                     /locus_tag="A7J05_RS09210"
                     /old_locus_tag="A7J05_08825"
     CDS             7488..8231
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385411.1"
                     /locus_tag="A7J05_RS09210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08825"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_076684018.1"
                     /transl_table=11
                     /translation="MRSRISNGTGLVVTGLVATLVALLFPLWSYEDRSGTGLDRLDAET
                     VATDFGPLTALDRDFVAKVRLAGLWELPAGRQAEERGTTKAVRTAGEHLVAGHTFLDAR
                     VREVAARLRLELPDQPGRQQRQWLAELSAARGGTYDEKFADILRRAHGAVFPVVAEVRA
                     GTRNSLVRTLADDANTTVLDHITVLEKTGLVDFDGLARDAAPDQAPATRSPAPPGPTAS
                     PAPASPATPSPMYPLPPAASRPKPE"
     misc_feature    8260..8277
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.61"
                     /tool="antismash"
     misc_feature    8262..8277
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     misc_feature    complement(8287..8305)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.76"
                     /tool="antismash"
     gene            8340..9122
                     /locus_tag="A7J05_RS09215"
                     /old_locus_tag="A7J05_08830"
     CDS             8340..9122
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125238.1"
                     /locus_tag="A7J05_RS09215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08830"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_076684019.1"
                     /transl_table=11
                     /translation="MFWVPLLLLAWAATGLTCGRLCVAAARAATPGKAAPTGRRLTLYE
                     AAFLSGGPARVADLALVAMAGARRLLIAHTRWATVVDPEGRDDIERSVLGAIGPAGQSR
                     IAPIRRGAAGADAVRALADRLVAAGLAVPEGAGEGVAAGVRQVRAAAVAVLALGAVAVL
                     MPGQEPAGAGETPVAAWFALPLLLCAGCLLIARVEVHPYTRWASPAGQALLRTLAAGDD
                     RLTAVAVRGVSAVDDPGLRAAFSHRAGRTGREAAQRGH"
     gene            9301..10905
                     /locus_tag="A7J05_RS09220"
                     /old_locus_tag="A7J05_08835"
     CDS             9301..10905
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (109-472). E-value:
                     1.3e-20. Score: 66.0. Matches aSDomain:
                     nrpspksdomains_A7J05_RS09220_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432060.1"
                     /locus_tag="A7J05_RS09220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A7J05_08835"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_076684020.1"
                     /sec_met_domain="PF00561 (E-value: 2.2e-26, bitscore: 91.8,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAAARYGTAGTLALSALVVAPAQSASGATAPSAADRGVVVAAE
                     RAEAAGIRWGACPKAEDMPPSLKCGTVIVPLDYARPGGKQIRLTVSRARATGGDGRGKV
                     ARQGSLVYNPGGPGGSGMHFPVMGIMPEWKRVAAAYDLVGYAPRGVGRSAPLSCQNPER
                     FIKAPTSAPSHPGAAYKKARIAKAKAHARGCAARNPGLRHYTTLNNARDLDVLRAALGE
                     RRLTFVGASYGTYLGAVYATLFPSHVRRMVFDSAVNPDPAQIWYRNNLDQSAAFERRWA
                     DFRAWMAKHHAAYGLGRTPEAVMRSYDRANRKLAREPAGGKVGPGELQAAFLQAGYYDD
                     YWPMRAAALSQYLKGNPEPLVEQAAPEPRAAAEAENVNAVYTAVECNDAPWPTDFGTWD
                     RDNSLLARSAPFETWENVWMNLPCAYWPAPRQQPVDVRTAEGELPPTLILAGERDAATP
                     YEGALEMRRRMGGSALVTERGTGTHGIGGGGNACVNRHLEAYLLKGRVPARDASCAPRR
                     EPEPQTAERAARPAEAL"
     aSDomain        9628..10716
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A7J05_RS09220_Abhydrolase_1.1"
                     /evalue="1.30E-20"
                     /label="A7J05_RS09220_Abhydrolase_1.1"
                     /locus_tag="A7J05_RS09220"
                     /protein_end="472"
                     /protein_start="109"
                     /score="66.0"
                     /tool="antismash"
                     /translation="LVYNPGGPGGSGMHFPVMGIMPEWKRVAAAYDLVGYAPRGVGRSA
                     PLSCQNPERFIKAPTSAPSHPGAAYKKARIAKAKAHARGCAARNPGLRHYTTLNNARDL
                     DVLRAALGERRLTFVGASYGTYLGAVYATLFPSHVRRMVFDSAVNPDPAQIWYRNNLDQ
                     SAAFERRWADFRAWMAKHHAAYGLGRTPEAVMRSYDRANRKLAREPAGGKVGPGELQAA
                     FLQAGYYDDYWPMRAAALSQYLKGNPEPLVEQAAPEPRAAAEAENVNAVYTAVECNDAP
                     WPTDFGTWDRDNSLLARSAPFETWENVWMNLPCAYWPAPRQQPVDVRTAEGELPPTLIL
                     AGERDAATPYEGALEMRRRMGGS"
ORIGIN
        1 tgcggaagcg cgggtaccgc ttcgtccgcg tctcggagca gatcggctcg gcgggggcgc
       61 ggcgttaacc tggccgtatg gccgacgacg agagttactg cgccgctccg ggcacggaca
      121 cggccgcggc cgtgcgcgcc ctgggcctcg ggcccgacgc cgcggccgag ggcccgttcg
      181 ccgcgtgcgt gctctgccag gagcccacgg agtacccgga gtcccacaag ggcgccaccc
      241 tgtgcccggt ctgcgcctgg caggaggccg agcggcaggc gtgctcgggc tgacgccggg
      301 ttacgcggcg cgcttcaggc gtacgagcac gggggcgccg aaggggtcgc cggactgggt
      361 cacctggacc ttgcccgcgc ccagccatgt gtccgtgccg ggcacccggt ggatctcctc
      421 cagccacagc gtctgaccgg agcccttgcg ggcccccgtc gacacgggca ggcggtggcg
      481 tttgatcttc aggcaccggc cgtcgtcgtg caggaaccag ttgccgtcga ggaacaggcc
      541 ctcgtcgtcc tgggccaccg ggacccgcag cgcgcacccg ccgggcgcct cctcctgctc
      601 gacccaccgg tcgtccgcac ggcggagccg gatgaactcc tcctggggtt cgtcctcgac
      661 ctcgccgtgg ttgaaggtgt tgttgccgta cgcgcgcacc tcgccgtccg cgagcgcgaa
      721 gaccttgagg agacccgcgc ccggctccgg cgggaggggc tcgtcgaagc gggactgcgg
      781 ggtctccacc tgctgccacg acgtgccgtc ccagtgcgac gacgcgggct ggtcgtacgg
      841 ctcgccgtgg acctgttcgg tgccggggcc cgtcgaacgg ctgccgaccg cccacacgtc
      901 gtccggtccc gccaccgcga ggtcgacggt cctgtggggc agtcgcgtcg tggtccagcg
      961 gctgccgtcg aagtgcacgg cggtccgctc gcccgtgagg gcccagatgt cccgcggccc
     1021 cgcggcctcc aggtccccga cgttgcccgg cggcgggttc ggcaccgcgg accagcggac
     1081 gccgtcccag cgcacccagg gggtgacgcc gtcgcccgtg gcgtacttct gcggccgcag
     1141 ccacagggtg tcgtcgccga tctcttcgag cacgggcggg tagtcggtgt cgccgagggc
     1201 ctcgggcagg ggctcacgct tccaccgttc gccgtcgtag tgcagcagat gggcgttgga
     1261 ggcgccgttg ttctcggtgc cgaccgccca gatgtcgtcc tcgccgaggg cggcgacgtc
     1321 ggtgaagccg ccgctgaagt ccgcgatgtg ctcgaactcc cagcggggcg aggcgctttc
     1381 ggggcgtgag gcgccggagc cgccggtccg ggagccgtcc ggctcggagc cgttgccgca
     1441 ccccgaggcc gtcagcagga cggccagcac cgcgcaggcc gccgcgcggg ggcgcctgac
     1501 gcccatggcc cggccgactc cgctggttcc gtcggctcca cgcatccggc gccctccccg
     1561 ttgtcgctcg ttgggtgttg ctgatgttgc tggtgttgtt gccgtgcaga aggcgggcag
     1621 cctatccccc ctcgcccccg ggcgtgcccc gcgccccggg ggtcagccgc gccgggagct
     1681 gagggcgcag gcggaccaga acgccgccac ggccacggcg agggccgcga gcaccgcccc
     1741 ccacggcacc ggcacggtcc cgtcgcgtga ccccgtggtc aggtcggtga ggaccgactt
     1801 cgccggggac cccgtggtga cgagcagcag cagcgcgccg agcagcatgg cgggcacggc
     1861 cttccccgcc gagcggagca gcggccggct ggtgagcgcc ccgactgccg tgccgagcag
     1921 ggcgctgacc agcatcgcga gcagtcctgc ggcgcccgcg ggcagccggg gcacggacag
     1981 cctggcgttg gtgctcgccg cgtcgctgac ggcggtcacg acgagcaccg ccgtcacgcc
     2041 gagcgccacc gccgcgagga acgcggtcag cagcgcggcg aggtgggcgc gcgccgggcc
     2101 ggccgccgcg gcggtgcagg cgcgggcggc gggcggttcg ctcccggcgc agatccgcac
     2161 cagccaggcg gcgacgggca gcagcacggc ggcggcgtag gcgagcgagt ccaggacggg
     2221 ctgcccgctc tgcacgccga cgccgaggaa gacggcgtac aggatgacgg gcgcgagcca
     2281 ccgctgggac cgtgcgagca gggcggcctg gtaccgcagg agcgcggtca ccgggaggtc
     2341 ccctcttcgg cggcgggcgc cgaggtgggg gacgggacgg cgcccggctc ctccacccgc
     2401 accacgtgcc agggcacccg tgccgtcagg agttcacgca ggaccaggtc ggagcggctc
     2461 tccgggaccg tgagccgcgt ggcccccgcc gcgcccccgg cgccgacgcc gaccgcgacg
     2521 gccgcgaccg gcgcggggag cgccgcgccc ggcggcccct gcgcgacgat gacgacgacg
     2581 ggaccgcgcg gcccggcgcc ctccgggccc ggcggctccg tgcgccgttc gagtccgccg
     2641 cccaccaccg cgtacgtctc gtccgccacg tccgcgaggc ggcgcgggtc gtggtcgacg
     2701 aagacgacgg agccgccggc ggcggtgcgt tcggcgaccg cgcggtcgag ttcggcgcgg
     2761 gcggggacgt ccaggcccgt ccaggcctcg tcgaggacca gcagctcggg atcggcgagg
     2821 agggcctgcg ccacggccac cttctggctg ccgcccttgg acagttcgga cagcggggtg
     2881 cgcgcgtggc cgccggcgcc gaagcgctcc agccaccgct cggcgctccg cccggccgcg
     2941 ccgcgcgcga ggccgtgcac gcgggccagg tgcgtgaggt agccgacggc ggtgaacggc
     3001 agcgccgcgg ggaagcgttc gggcacgaac gccgtgcgcg ggcgttgggt gagccggccc
     3061 tcggagggcg cgtcgacacc ggcgagcagc cgcagcaggg tcgacttccc ggtcccgttg
     3121 ccgccctgga cgcgtatcag ggcgcccgcc cgtatgccga ggtccacccc ccgcagcacc
     3181 caggggccgc gcagcccgta acggcggccg acgccgctga gccggagcac ggggtcacgt
     3241 tccatgcggg ccatcctcgc gtacgcggcc gacgccccgc ggggcggatt ccccgcctgg
     3301 cagactgggc ccgtgagcag cgacgagacg acttccaccg gcacgcggga cggcgggccc
     3361 gccgcggaca gccccttccg ccacgagagc acaccgcgcg acgaggcccc gcagttcgtc
     3421 ctgcccctcg tcgcccgcat cgagcgtgcc gctcccccgg cccgcacgga cgcgttggag
     3481 accgcggccc gtgccgtcct ggtgatgctg agcgacgagc gttcgctcgg cgacggcgag
     3541 tgggcgcggg cgatgcgcga ctggcaggac gcccgcatcc gcaaggtggt gcgcagggcg
     3601 cgcgggggcg agtggcgcaa ggccgagacg ctgcccggca tcacggtcac gggcaagtcc
     3661 gcggaggtgc gcgtcttccc gccggtcccg ctggacggct ggcccaagga gctggcgaaa
     3721 ctccaggtgt cgggcaccga cctggacgac ccggagccgc cgggcggggc cgacgcgacg
     3781 caccccgtcc tgtggatgag ccccgatgtc gacatgtcgg cgggcaaggc gatggcgcag
     3841 gcgggccacg gcgcccaact cgcctggtgg gagctgtccg ccgaggaccg cacggcctgg
     3901 cgcgacgccg gcttccccct ctcggtccgc acggccgccc ccgcccagtg gcgggcgctg
     3961 accacgagcg gcctgccggt ggtccgcgac gcgggcttca cggagatcgc cccgggctcc
     4021 tgcacggtgg tcgccgacca cccggcgctc cgcacgcccc gccactgaac gccccgcccg
     4081 tgtcttccgt actcctcggt atcaccggtg agcacgggag gcaccttgcg gcacgtcaac
     4141 ggcacggctc tgatcatcgc ggcactcgtc gccacgctgg gcgcgatcgc gttccccgtg
     4201 tggtcctacg ccgaccgctc cggcacgggc gaggccgacc tcgccgccgg gacggtgccc
     4261 acccggtggg ggccgctctc ggcggccgac cgggatctgc tggtgaaggt gcggctcgcc
     4321 gggctctggg agatccccgc gggccagcag gccgtggagc gcgcgccgac cgaggccgtc
     4381 aaggaggccg gtgaccacct catcgtgggc cacaccgacc tcgacaagcg ggtgcgctcc
     4441 gccgccgcgc agctcggcgt ggagctgccc aaccagccga ccgcccagca gcagggctgg
     4501 ctggacgagt tgacggaggc gagcggggag acgtaccagc gcacgttcgc caatctgctg
     4561 cgcgcctcgc acggcaaggt cttcagcgtg atcgcccagg tccgcgacac cacccgcaac
     4621 tcgctggtca ggcagctcgc cacggactcc aaccagaccg tcctcgacca catcacgatg
     4681 ctggaggcca ccgggctcgt cgacttcgac gacatcgcca acggcgccgc ggccgacgcc
     4741 accgagagcc ccaccgggcc tcctcccccg ccgtccgggg ccgccccgcc cgaggctccg
     4801 cccgccgggc ccgagggcga ggtgtcgacc acgtcgcggc cctcgccggg gccgccggga
     4861 cgcatcgaca ccggccggcc ggaacctcaa atgtagctct gaacgtctcc cctgcggggt
     4921 tcggccggtc ccgtcccggt gatggtcccg gggacgagtg accgggcgac cggggaactc
     4981 ggggggacga agggggagaa atggagcgtc tggggaccgg tatcggctgg cgtcctgaga
     5041 tcgcggacgc cgtggaggcg atgcccggca tcgactgggt cgaggccgtc gcggagaaca
     5101 tctgtccggg gcatctgccc gactcgctgc tgcggctccg cgagcgtggc gtgaccgtcg
     5161 tgccgcacgg tgtctcactg ggcctcggcg gcgcggaccg tcccgacgag gggcgtctga
     5221 cggcgctcgc cgagcgggcc gaggccctgg gctcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtacgggc cggtggcgcc ctcaccgcca cccagcccct cgaagccggg cacctgctgc
     5341 cggtgccccg cacccgcgac gcgctcgacg tgctctgcga gaacgtccgc gccgcccagg
     5401 acgcgctgcc cgtgccgctc gccgtggaga acatcgcggc gctcatcgcc tggcccggcg
     5461 aggagatgac ggaggggcag ttcctgtacg agctggccga ccgcaccggt gtgcggctgc
     5521 tcatcgacgt cgccaacctc cacaccaacc acgtcaaccg cggcgaggac ccggtcaagg
     5581 ccctggaaga actgccggtc gaggccatcg cgtacgtcca tgtcgcgggc ggcttcgagc
     5641 gggacggggt ctggcacgac agccacgccc atcccgtccc cgagccggtc ctcgcgatcc
     5701 tggccgacct cgcgtcgcgc accgcgccgc ccggcgtcct gctcgaacgg gacgagaact
     5761 tccccggacc ggccgagctg gagcgcgaac tcgacgccat ccgggccacg gtgaagacct
     5821 cccccgccga gccgggcgcc gcgccggaca ccgtgccgcc cgcctccgcc gccgcccggc
     5881 agcgcaccgc actcgcccag gccgccctgc tctccgcact cgtggcgggc accccggcgc
     5941 ccgagggctt cgaccacacg cgcctgaagg tgcagagccg cgcgctgggc gccaagcgcg
     6001 ccgacgtcgt cgcgaaggtc gcgccggagc tgccggagat cctcggcgcc gcctaccgcg
     6061 ccgagttcct cggctacgcc cgggtcaggc cgatgaccgg cggctaccgc cacgacgccc
     6121 tcggcttcgc cgagcacctg ctgctcgcgg ggcggcccgc gtcaccggac gcccggcagc
     6181 ggctgagcga ctggtggctg gaccgctcgg gcccggcgcc gcgctcccgg cggcccgccg
     6241 cccggctgct gcacgccacc cgccgcgtcc tgctgcggcg gtgacgccat gagcgtcgcc
     6301 gcggtcgtgt acgcgaccgc cgtcctgctc tcctgcgtgc cgctggtcat gggcgtggtg
     6361 acggtccgcc gcggcgggtc ccggcacggc gagggctccc gcgccggtga cggggccttc
     6421 gtgcggggca ccccggaggc cgcgttcctc tccggcggcc ccgcccgcgc cgccgacgcg
     6481 gtcatcgcgg ggctgcacga ggacggccgc ctggtcgtcg cgggccccgg cgtcgtcggc
     6541 atcacgcggc cggtcgcgcg caacgccgcc gagcaggcgg tcatcgacgc ccacacggcg
     6601 gcgccgagcg gtgcgctgca ctggctgcgg gccgccgtga tgcgcagccg cccggtccag
     6661 gagacgggcg acgcgctggc ggagcggggc ctcgtcgtgc gccccgggcc gcggcgcacc
     6721 tggcggcgct gggcgggggc tcaggtcttc ctgaccttca tcgggctccc gaccgcgggc
     6781 atcctgacgg cgctgcagta cgccgatgac gactacacgg agccggaccg gctgctcgac
     6841 agcgtcctgc acgtcctgct ccccgccgcc gccctcggca tcggcgtcgg cgtcgtctcc
     6901 ctcaccctga cgtccggtca gctcacgccc gccggccgcc gcgccctcca ccagtacgtg
     6961 gactcgtccc ggcacctgac gggcgccgcc cacctcgtgg ccacccgcgg gctccccgcg
     7021 gcccaccccg acctgcggtc ccagctcatc gccgcggcca gggcccgggg cctcgcggcg
     7081 cccccctcgt ccccggcgcc gctgccgttc caggacccgg gcctctggag cgccggggcg
     7141 gcggcctggt gcgggtccac caccggcttc ggcggcgcgg gcggcggctc cgcgtgctcc
     7201 ggcggcggtg gcggcgcggc ctgttcgggc agcgggagcg cgtgctcggg aggctcgtcc
     7261 tgctcgggcg gcggctcgtc ctgtggcggt ggcggtggcg gctcctcctg cgggggcggc
     7321 gggggcggcg ggtcctgttg aagcagtccg ccctcctccc gacgggcggc gcacgccgcg
     7381 ccgcccgggc cggtggcgcc gtacggaggt tggcgtacgc cccgccgtaa cgccgccccg
     7441 ggccgcgcgg ggggcactag cgtgcggtgc cgcaccagga ggcgcccatg cgatcccgca
     7501 tcagcaacgg caccggtctc gtcgtcacgg ggctcgtcgc gacgctcgtc gcgctgctct
     7561 tccccctctg gtcctacgag gaccgctcgg gcaccggcct ggacagactg gacgcggaga
     7621 cggtggcaac cgacttcggg ccgcttaccg ccctggaccg ggacttcgtc gccaaggtgc
     7681 ggctcgcggg cctgtgggag ctgcccgcgg gccggcaggc cgaggagcgc ggcaccacca
     7741 aggcggtgcg gaccgcgggc gagcacctcg tggcgggcca cacgttcctg gacgcgcgcg
     7801 tacgcgaggt cgccgcccgg ctgcgcctgg aactgccgga ccagcccggc cggcagcagc
     7861 ggcagtggct cgccgagctg agcgcggcgc gcggcgggac gtacgacgag aagttcgccg
     7921 acatcctgcg ccgggcgcac ggcgcggtgt tcccggtggt ggccgaggtg cgcgccggca
     7981 cccgcaactc actggtgcgt acgctcgccg acgacgcgaa cacgaccgtc ctcgaccaca
     8041 tcaccgtcct ggagaagacc ggcctggtgg acttcgacgg cctcgcgcgg gacgcggccc
     8101 ccgaccaggc ccccgcgacc cgctcccccg cgccgccggg ccccaccgcg tcaccggccc
     8161 cggcctcgcc ggccaccccg tcccccatgt atcccctgcc gccggccgcc tcccgcccga
     8221 agccggagta acccccgcca tcacccggcg ggaaacggaa cgttacctac ccgtcaccga
     8281 ccgtcccgca tgctgaatat gccatggcgc gaagcgacgc cttcgcatag aacaccgcca
     8341 tgttctgggt ccccctgctc ctcctcgcct gggccgcgac cggcctgacc tgcggcaggc
     8401 tctgcgtggc cgccgcccgt gccgcgacgc cggggaaggc cgcgccgacc ggccgccgtc
     8461 tcacgctcta cgaggcggcg ttcttgtccg gcgggcccgc gcgcgtcgcc gatctggcgc
     8521 tcgtcgcgat ggccggggcg cggcggctgc tgatcgcgca caccaggtgg gcgaccgtgg
     8581 tcgacccgga ggggcgcgac gacatcgagc gttcggtgct cggcgcgatc ggcccggcgg
     8641 ggcagtcccg catcgcgccg atacgccgcg gggcggccgg cgccgacgcc gtgcgggcgc
     8701 tggcggaccg gctggtcgcc gcgggcctcg ccgttcccga gggcgcgggc gagggcgtgg
     8761 cggccggggt gcgccaggtg cgggccgcgg cggtggcggt gctcgcgctc ggcgccgtgg
     8821 cggtgctcat gccgggtcag gaacccgcgg gcgccgggga gacgccggtc gccgcgtggt
     8881 tcgcgctgcc gctgctgctg tgcgccggct gcctgctgat cgcccgcgtc gaggtccacc
     8941 cgtacacgcg ctgggcctcg cccgcggggc aggcgctcct gcggacgctc gcggcgggcg
     9001 acgaccgcct cacggccgtg gcggtacggg gggtgagcgc cgtcgacgac cccgggctgc
     9061 gcgcggcgtt ctcgcaccgg gcgggccgga cgggccggga agcggctcaa cggggtcatt
     9121 gagccgacac cgacgggccg gtgctttact tcaccgacta tcggacgaat catcccctgt
     9181 gtttgacacc cacgccgacg ccgctgccga tgccgacgcc cgacggcgag ccctcgcggg
     9241 cgcgcgcgga cgtcaccgcc tgcgggaacg cacgcccgaa ccgaaggggt catcagcgcg
     9301 atgagagcag cagccgcccg ttatggaacc gccggaaccc tggccctgag cgccctcgtc
     9361 gtcgcccccg cgcaaagcgc gagcggagcc accgcgccgt ccgccgcgga ccggggcgtc
     9421 gtggtcgccg ccgagcgcgc ggaggccgcc ggaatccgct ggggcgcctg ccccaaggcc
     9481 gaggacatgc ccccgtccct gaagtgcggg acggtcatcg tgccgctgga ctacgcgcgg
     9541 cccggcggca agcagatccg actgaccgtc agcagggcgc gggcgaccgg cggggacggg
     9601 cgcgggaagg tcgcgcggca gggctcgctg gtctacaacc cgggcgggcc cggcgggtcg
     9661 ggcatgcact tcccggtcat gggcatcatg ccggagtgga agcgcgtcgc ggccgcctac
     9721 gacctggtgg ggtacgcccc gcgcggtgtc ggccgctccg ccccgctctc ctgccagaac
     9781 cccgagcggt tcatcaaggc gcccacgagc gcgccgagcc accccggcgc cgcgtacaag
     9841 aaggcgcgca tcgccaaggc gaaggcgcac gcgcgcgggt gtgccgcccg caacccgggg
     9901 ctgcggcact acacgacact gaacaacgca cgcgacctcg acgtgctgcg ggccgcgctc
     9961 ggcgagcgga ggctgacgtt cgtgggcgcc tcgtacggca cctacctcgg ggccgtctac
    10021 gccacgctct tcccctcgca cgtgcggcgc atggtcttcg actcggcggt gaaccccgat
    10081 cccgcgcaga tctggtaccg caacaacctc gaccagtcgg cggcgttcga gcggcgctgg
    10141 gcggacttcc gcgcgtggat ggcgaagcac cacgcggcgt acggcctggg caggacgccg
    10201 gaagcggtca tgcgcagcta cgacagggcg aaccggaagc tggcccgcga gccggccggc
    10261 ggcaaggtcg gcccgggtga actccaggcg gccttcctcc aggccgggta ctacgacgac
    10321 tactggccga tgcgcgcggc ggcgctctcg cagtacctca agggcaaccc ggagccgctg
    10381 gtcgaacagg cggcgcccga accgcgggcg gcggcggagg cggagaacgt caacgccgtc
    10441 tacacggccg tggagtgcaa cgacgcgccc tggccgacgg acttcgggac ctgggaccgc
    10501 gacaactcgc tcctcgcccg ctcggcgccg ttcgagacgt gggagaacgt ctggatgaac
    10561 ctgccgtgcg cctactggcc ggcgccccgg cagcagccgg tcgacgtccg tacggccgag
    10621 ggcgagctgc ccccgaccct gatcctggcc ggcgagcgcg acgcggccac cccgtacgag
    10681 ggtgccctgg agatgcgccg caggatgggc ggctcggccc tggtgaccga gcgcggcacg
    10741 ggcacgcacg gcatcggcgg cggcggcaac gcctgcgtca accgccacct ggaggcctac
    10801 ctcctgaagg gccgcgtccc ggcgcgggac gcgtcgtgcg cgccgcgccg ggagccggag
    10861 ccgcagaccg ccgagcgggc cgcgcggccc gccgaggcgc tctgagggac cctcggatct
    10921 aggccaggcc cgccacgagg tcggcgacgg acttgcggcg gccggtgtag aacggcacct
    10981 cttcacggac gtgcatccgg gcctcggagg cacgcaggtg gcgcatgagg tcgacgatgc
    11041 ggtacagctc gtcggcctcg aaggccagca tccactcgta gtcgccgagc gagaacgagg
    11101 cgaccgtgtt ggcgcgcacg tcggggtagc cgcgggccat cttgccgtgg tcggcgagca
    11161 tgcggcggcg gtcctcgtcg ggcagcagat accagtcgta ggagcgcacg aaggggtaga
    11221 cgctgatgta gttccgcggc gtctcgtcgg ccaggaaggc cgggatgtgc gacttgttga
    11281 actc
//
