LOCUS       NZ_BDDX01000012        19484 bp    DNA     linear   CON 23-AUG-2024
DEFINITION  Apilactobacillus kunkeei strain FF30-6, whole genome shotgun
            sequence.
ACCESSION   NZ_BDDX01000012
VERSION     NZ_BDDX01000012.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Apilactobacillus kunkeei
  ORGANISM  Apilactobacillus kunkeei
            Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
            Apilactobacillus.
REFERENCE   1
  AUTHORS   Maeno,S., Tanizawa,Y., Kanesaki,Y., Kubota,E., Kumar,H., Dicks,L.,
            Salminen,S., Nakagawa,J., Arita,M. and Endo,A.
  TITLE     Genomic characterization of a fructophilic bee symbiont
            Lactobacillus kunkeei reveals its niche-specific adaptation
  JOURNAL   Syst Appl Microbiol 39 (8), 516-526 (2016)
   PUBMED   27776911
  REMARK    DOI:10.1016/j.syapm.2016.09.006
REFERENCE   2  (bases 1 to 19484)
  AUTHORS   Endo,A., Tanizawa,Y., Maeno,S., Arita,M. and Seppo,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2016) Contact:Akihito Endo Tokyo University of
            Agriculture, Department of food and cosmetic science; yasaka 196,
            Abashiri, Hokkaido 099-2493, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 0.7
            Genome Coverage                   :: 123X
            Sequencing Technology             :: Illumina Genome Analyzer II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001949975.2-RS_2024_08_23
            Annotation Date                   :: 08/23/2024 20:17:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,533
            CDSs (total)                      :: 1,465
            Genes (coding)                    :: 1,459
            CDSs (with protein)               :: 1,459
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:23:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 19484
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BDDX01000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..19484
                     /db_xref="taxon:148814"
                     /mol_type="genomic DNA"
                     /note="scaffold: sequence12"
                     /organism="Apilactobacillus kunkeei"
                     /strain="FF30-6"
     region          1..19484
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..19484
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..19484
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[9354:17656]"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      9355..17656
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     gene            565..927
                     /locus_tag="FF306_RS05455"
                     /old_locus_tag="FF306_01078"
     CDS             565..927
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025107.5"
                     /locus_tag="FF306_RS05455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01078"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_094750990.1"
                     /transl_table=11
                     /translation="MTEDDKDMTELYANWRKDQYHENKGFFPIFQSFSSKMTKLSNGAI
                     ALYVFLGLKSNYKTGVSFYSVKKLSIIFNKSPRTISFWIKDLEDNKLIYRKQKTLNGVS
                     TTYLLPYSDKNKDTNF"
     gene            978..1556
                     /locus_tag="FF306_RS05460"
                     /old_locus_tag="FF306_01079"
     CDS             978..1556
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292173.1"
                     /locus_tag="FF306_RS05460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01079"
                     /product="recombinase family protein"
                     /protein_id="WP_094750991.1"
                     /transl_table=11
                     /translation="MIYGYARVSTLGQSLDNQIQDLRDNGVLKKNIFSDKYTGTSTDRP
                     GFQNLLDTLKPDDTLVVTKLDRLARNTREALNVINELQDKNIKINILNLGIVDNSSIGK
                     FTTTVLLAVAEMERDMIVNRTQEGKKYAKKHNPNYREGRRTRLTGKNKDKYPAIYEYKQ
                     SHTAEETAKAFGTSVRTVFYIQSIFKKQK"
     gene            complement(1641..2507)
                     /locus_tag="FF306_RS07690"
                     /old_locus_tag="FF306_01080"
     CDS             complement(1641..2507)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FF306_RS07690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FF306_01080"
                     /product="hypothetical protein"
                     /protein_id="WP_094750992.1"
                     /transl_table=11
                     /translation="MKNHSKLCKVNIKIIGAMTLLTALLATPIERNVIASASSFHYQIK
                     KSGYVYKNAHIKSIKSHSAKYYFGSHKLIVKKTIVVKRHGKRLIYKYVLASNGRNGWVN
                     TKYVKKVKVKAAKHNKVVNTHKKTVTIPNIKKKTNTKVKPKLKLPHSDGHKKGQYVPNK
                     ENIKAELKKLLDPYLTQYGKIYGVTTGKMVEPIVDSNGYGSEFVVTSGNFVNSDKAMAK
                     ILFNGLLNGTLMTYLEDHSDVSIVIDHVDLDTSGGSIDARLYLTWYQDMSIQPKFEILN
                     GKRVYHY"
     misc_feature    complement(2536..2549)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.07"
                     /tool="antismash"
     gene            complement(2559..3032)
                     /locus_tag="FF306_RS05475"
                     /old_locus_tag="FF306_01081"
     CDS             complement(2559..3032)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FF306_RS05475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FF306_01081"
                     /product="hypothetical protein"
                     /protein_id="WP_094750993.1"
                     /transl_table=11
                     /translation="MSDEISKSVQDMIKRNRESGKKLEEVGRKYNDSNENFVDLDSANY
                     ILESNQDTPIFQKDDEKKKSKKEYDKGFVSMKLKKEIAEKLDHVINFNTSSTHITLKRG
                     EMISKIIDFYIFTVIRNAGSEDKAKKNLISANEAEKFYHFIREMVENQDDSEF"
     gene            complement(3327..3605)
                     /locus_tag="FF306_RS05480"
                     /old_locus_tag="FF306_01082"
     CDS             complement(3327..3605)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FF306_RS05480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FF306_01082"
                     /product="hypothetical protein"
                     /protein_id="WP_094750994.1"
                     /transl_table=11
                     /translation="MNLNKKNNLVLGLEFVSYIYMSVLLWFSDSDYKKGMMFLCLMFLA
                     GSFSRFYKFKRDISDDDIESVNKGLNRLIFVCIAAPISLTIAVVCFN"
     gene            3866..4666
                     /locus_tag="FF306_RS05485"
                     /old_locus_tag="FF306_01083"
     CDS             3866..4666
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FF306_RS05485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FF306_01083"
                     /product="hypothetical protein"
                     /protein_id="WP_094750995.1"
                     /transl_table=11
                     /translation="MDNLKVLGRMSGFPYKHEKIVAVLESKKDTFENTRYTVFSTGTHI
                     KVLEISDRITSQRNISFSEFIEKCHIDERSVREFNDEYGIRLTDNITFDEIRFGVHLLE
                     AIYDDDYTKSLVGKKLFIRSLVSDHYTVKAAESLEYGKILKVTYDNGDHDFFINELPVF
                     KVDMYTPYFANKLSKATSPDTAYMIVFNSRYHLKNRQGLIDDYLEKIPKNKQQLTVINR
                     LKSESFEKLCLERLKDNSRISNYKIFKMDDQDFVFYKISAVRYY"
     gene            complement(4948..5814)
                     /locus_tag="FF306_RS05490"
                     /old_locus_tag="FF306_01084"
     CDS             complement(4948..5814)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036540.5"
                     /locus_tag="FF306_RS05490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01084"
                     /product="hypothetical protein"
                     /protein_id="WP_188595232.1"
                     /transl_table=11
                     /translation="MIYVHIIALNPLKINIKGALIMKFSKLFEEFSDAGLSPAEQVALT
                     RLYDRMSLSEGNSDFYDKQQNAYYVTYSREDMADKLNVSTNTVTNIFNSLKRKGWLIVK
                     QRFNSTNRIFLPISLKTKNCTTKTQKLVSNKTEYNNTNKKISHNKNDTKKSVESLKLDG
                     IAQSLINKAGFSPLLVSQLKALSFNKPDTLYDYGKLILKAKRMALRFSVAKNSSLRRFE
                     TNPLLNEYLENHTEPIIRNANRKAKNRYGYMIKSFKNLFEEAIDNYSQHIHYNNDIERL
                     FPENIVF"
     gene            6283..7095
                     /locus_tag="FF306_RS05495"
                     /old_locus_tag="FF306_01085"
     CDS             6283..7095
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807713.1"
                     /locus_tag="FF306_RS05495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01085"
                     /product="ParA family protein"
                     /protein_id="WP_094750997.1"
                     /transl_table=11
                     /translation="MTRIITLGNFKGGVGKTTSASLIAYNLSDEGYKVLLGDLDPQGNA
                     TNMMLKTKANFDGKVSSFRQTLMKSVEEEDLSKSIITITDKLDLLASAPDFALYPRYME
                     KIADYNKRVQYLKTLFDKIKDNYDYIILDTPPTIMSLFSDSALYMSDWCLIVMQTHKDS
                     FDGSKAFIDYLQEYVVDAYKAPKLDLVGILPVLIKNNAPVDEYTLQSAKDQFGAENILD
                     HHIKHMERIKRFPITGIADHELWDKRVIQAYKEVTDEMLDRIRKEEAE"
     gene            7092..7412
                     /locus_tag="FF306_RS05500"
                     /old_locus_tag="FF306_01086"
     CDS             7092..7412
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036624.5"
                     /locus_tag="FF306_RS05500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01086"
                     /product="DUF5388 domain-containing protein"
                     /protein_id="WP_094750998.1"
                     /transl_table=11
                     /translation="MSSLINSNRKDDVKPLKSATLADLEQEKSDEQPKKEVKKEPAYKG
                     HKTTLSIDSHTKNMLQVMVLLGDAPNQKEGFSVAINAYYDNLTQDKKNMFDMMLKQLNS
                     HD"
     gene            complement(7611..8975)
                     /locus_tag="FF306_RS05505"
                     /old_locus_tag="FF306_01087"
     CDS             complement(7611..8975)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.5"
                     /locus_tag="FF306_RS05505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01087"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_094750999.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.3e-34, bitscore:
                     110.0, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNRKISSPIKLCIVFVISFVLILIYSVDAKANASSTYESLSNSR
                     IQYKKVLQLIKKKKGKIIYDIPEIYYVRFRIDKDKDKDKHNILHHKTIEKKKTNIKIWR
                     KNNYTPEYKFKKPKYWNKQWDIKAITLKGKMYKHVLYSPSVTIGVVDSGIASNQSSLNG
                     TIIKKGSVNLVPKGGFNGKENDEYGNKGYIYDKIGHGTMIAGQISGHGKVIGVYPKVGL
                     HIYRVVGKKQSNPFWIMKGIISATNNKDSVINVSLGKYLYSSKSLKKNMEFKAWNRTIK
                     YSKKHGSIIVASAGENGYDENNIHRFKKSFSKDNNISVKKIKKLVDVPALLKNVISVGS
                     LGPSGNESNFSNRSKNISLFAPGGDTTEYFKYGSNYWNSHNLNSKKLITVATIKNSYSY
                     AYGTSLASAKVSALLAEYIAIQRRHHNYKNIKSRFLKYFKSYNKKVNGNIIIDNN"
     gene            complement(9082..9291)
                     /locus_tag="FF306_RS05510"
                     /old_locus_tag="FF306_01088"
     CDS             complement(9082..9291)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03731.1"
                     /locus_tag="FF306_RS05510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01088"
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="WP_371416534.1"
                     /sec_met_domain="TIGR03731 (E-value: 2.1e-17, bitscore:
                     53.2, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEVIDMSNFNDFNLGIKKVHSGSKKGLEPRITSYVLCTPGCITGR
                     LMGCNNKTKTCHCHSSNHFSFHAR"
     CDS_motif       complement(9202..9291)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEVIDMSNFNDFNLGIKKVHSGSKKGLEPR"
                     /locus_tag="FF306_RS05510_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(9082..9201)
                     /prepeptide="core"
                     /locus_tag="FF306_RS05510_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="9.10"
                     /molecular_weight="4164.8"
                     /monoisotopic_mass="4162.0"
                     /core_sequence="ITSYVLCTPGCITGRLMGCNNKTKTCHCHSSNHFSFHAR"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEVIDMSNFNDFNLGIKKVHSGSKKGLEPR"
                     /leader_location="[9201:9291](-)"
                     /alternative_weights="4182.8"
                     /alternative_weights="4200.8"
                     /alternative_weights="4218.8"
                     /alternative_weights="4236.9"
     gene            complement(9355..10668)
                     /locus_tag="FF306_RS05515"
                     /old_locus_tag="FF306_01089"
     CDS             complement(9355..10668)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017000.5"
                     /locus_tag="FF306_RS05515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01089"
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_094751001.1"
                     /sec_met_domain="LANC_like (E-value: 1.9e-54, bitscore:
                     174.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDETIDFIKKHSEKILNIDFIRENIDYLAKQSSNNNIDFFKQDL
                     SNGIIGLILLSEELNKQNIIHNKQNTDDLISILLDSIQKKPIMDASLFEGVSGIAFSLA
                     LLNRPDLCGVINQLNDFLFTLIDVKISEQKNRIHSPAYSYDFDTMYGLSGVLNYLVYFF
                     NNSNDENEKDIVGKRIIRIEKHIVDMYSTQLIYDDISVPAWFVSSDHQMDKRESINYPK
                     GNFNISFSHGISGLLYSLSNAYLSGLYVSNQLTLMNKMHTFIYDNLLVKNDVFCPPFFI
                     DFNSFTKEKKDSVERFDSWCYGTPGIDIALLKYNLIKRNPDEVDLMSRGLQEINKKPLG
                     IADPSMCHGYAGILAILNSLNKDYSLDIHLNKKIVKLLLSYFDNESITGFFSERFDDAE
                     TSRNIPDLGMLSGTSGIFLSLLSLKDKQYVHWGKIFGL"
     gene            complement(10661..12394)
                     /locus_tag="FF306_RS05520"
                     /old_locus_tag="FF306_01090"
     CDS             complement(10661..12394)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.5"
                     /locus_tag="FF306_RS05520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01090"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_094751002.1"
                     /transl_table=11
                     /translation="MKEIKQTIQLIWKQHKILFSILIFINIVLGILPLISIFFVQLIIN
                     FLSLSSHFSLILMCFAGYISVSLITDSISSVQSYINGIISNRLSFEVEYDILNVVSRMD
                     ITAFENEETYSVIDKLSNEAPSKPFSVFTAFNSISVSIITIISSYFYIFKINPIYGITL
                     VLLTFISIPFLLILSNNQFNVHWNRAEKERKAWYIKYLLTHDFSVKEIIFFDLSKHLKK
                     KFANLKKEFISQDLKILKNLSIFNYVYDLIVSIVTAILVLSVIWSIYLGKALLGTLTSV
                     TQIISLTQGSTQTFINNLFNLKYDLQILRKLYDFIDDNEKDDNGSYYNLPITTIEFKNV
                     SYSYKNSVKALDNISFKIKKGEIVAIVGANGSGKSTIVKILTGLYKPTRGQYLINNIDS
                     NKLNKHSFHKGISVLFQNFVQYELSVRENIGFGDIRNLKQEDKIMNTLKTYNDNLYKKL
                     KVEDSLGNWFGNGKQLSGGQWQSIATSRAFFNRNASFVILDEPNSALDPISESNLFYEF
                     KKFISHNKMGLFISHRIGAVKNADRIIVMSKGEIVAEGSHEYLLNNCTEYQKLEEAERY
                     E"
     gene            12510..13193
                     /locus_tag="FF306_RS05525"
                     /old_locus_tag="FF306_01091"
     CDS             12510..13193
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003649446.1"
                     /locus_tag="FF306_RS05525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01091"
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="WP_094751003.1"
                     /transl_table=11
                     /translation="MANILETKNVTKSFRNQLALDNVSVHIPKGEVYCLLGPNGAGKTT
                     LMKIITGMIHKTSGKVIFDGKEWERDALNDIGSLIENAPIYGNLTALENLKVVATLRKV
                     PLNHLESILEQVGLENNKKRSKDFSLGMKQRLGIGMAMVSNPKLLILDEPTNGLDPIGI
                     QELRTLIKGLAKKDITILISSHDLSEVEHLADNIGIISGGKLLLEEKYNNENLEKLFNE
                     IVGGV"
     gene            13196..13921
                     /locus_tag="FF306_RS05530"
                     /old_locus_tag="FF306_01092"
     CDS             13196..13921
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.5"
                     /locus_tag="FF306_RS05530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01092"
                     /product="ABC transporter permease"
                     /protein_id="WP_094751004.1"
                     /transl_table=11
                     /translation="MTSLINSEFLKIRRTSIPWLTILVPFFCVFLVAVVFAGNEYSMQS
                     IINQWSLIWVLLFSVLLSGMTTHHESISTQYKSILSSPVSLFKFELSRLIMLVELFFIG
                     NVFLAILVCISKFYFSSMVGMGYILLSIIMVSISGLWTVPFFLLISRLSNMFVGILIGL
                     FGFLIGGVFSGTRFGYLIPFTWSPETASPIIRMHVNGVPYSLGTHSFMNTTYIILSLSL
                     FIVLALTECLLFTRQEVKK"
     gene            13918..14655
                     /locus_tag="FF306_RS05535"
                     /old_locus_tag="FF306_01093"
     CDS             13918..14655
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR03733.1"
                     /locus_tag="FF306_RS05535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01093"
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="WP_094751005.1"
                     /transl_table=11
                     /translation="MIARVFKSEAIKLINSHILYFHIAAMVIFPTLLAVFFGKNHYPLT
                     NFVLIPNFFLIISFVGPVVISIVASMVNDRETKAGNYKNLLDTSISPLKVFQSQIAFYW
                     SLYLTEIILSFYIYGLILNIVFHAPLFPYFTLLVFCILFSLLAVIQYELCFLASYIFGT
                     AGSLILGFAGLILSALSETVLFDNVWEFIPWSWQIRSIAFFQNGTTINDSIHYAFLYIF
                     SILISLVSLFVCNKIFYRILNKN"
     gene            14666..17656
                     /locus_tag="FF306_RS05540"
                     /old_locus_tag="FF306_01094"
     CDS             14666..17656
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016615.5"
                     /locus_tag="FF306_RS05540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01094"
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_094751006.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.3e-74, bitscore:
                     240.8, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 5.2e-15, bitscore:
                     45.9, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTYYNVENPVVFRKHFCNVEGGEKFLYDESLLDYLSKFESFKEQ
                     IRVSNHELYKNYMDYLKDRSSFSKKRTRHLVESITGYFLRIKYRNTPFGLFSGVGVVNN
                     EKHTKYTHLKKIVLGYRWKYSVINMLEKRYRSNVKYVFENNMTDINGNPFFLDVGKESN
                     IIDGKYYNDEIGFKISKNKLTELLEKFLSKPRCTDDIFNVIKGEYKNIDPSYIKEYIVN
                     LVEKKIIKSTLRSSLSQTDEEWIENVKDFLKVVKDNQSIQKFETLKSLISSYSITNVGE
                     GIYLFDSISKCMNDIFPTQKDDLNVDLYLNDPSLSVTQDNLNDLNKCIDMLTKISIPYD
                     ELHDYTSLFLDKFGMDVEVPLYELIDKNSGIGFPKYDSSDNYSTSKETLESIGSLLDEK
                     LYTALINKRPLTIDQSTVNDVQRILNGSDSHKDIIPKSMDVCLTQESHDNKISFTISEI
                     FGSPLGSSITGRFRLLDDLYQKNKDVSDDYITCDINAFPNLLSLSTLTQNRHLNEYELS
                     VGTNGNQHKNQISLNDLVVGYENDTLYIRCSQNNKIVRFVDYNMLNGDLKNPVIKLLLA
                     ISNQSDRWPFNFPWSFGQPTRTVIPEIICNNIIVSPLTWNINTANYDNSFEDFYREFIC
                     KIDSLQVPKLFYFVESDNKIVSDRKKESIYNIYKHLKKSNSKNAKLQKCEFGSLNDDGK
                     TQEIVVTLKNKYKNNNPINQKPIYRSCDYLKPLNVVNGWFYVEVEFGDVGIKNEFIIQE
                     LSKLNDDLNNLYDLFFFIQYENKDNPTIRLRWKSNDIFIVQNRVTKYLDSLIKEGIIKN
                     YTNNNYFRELDRYGGKQNIDKAEQAFHYDTLFSIVSFQNNPEKIEKESLAIYSCVSMLL
                     SISNSIDTIIDFLNIYDYPYNINKNASGWIRKNEKYMDEYLNNLTIETKSLLDRRNNEI
                     NDIISNSDRNQVNIVSSFLHMSINRILGTDRYGEQQIMGILLKLFNKAKYTNEITGCKL
                     YTNNSES"
     gene            17893..19341
                     /locus_tag="FF306_RS05545"
                     /old_locus_tag="FF306_01095"
     CDS             17893..19341
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037255.5"
                     /locus_tag="FF306_RS05545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_01095"
                     /product="PIN-like domain-containing protein"
                     /protein_id="WP_143441330.1"
                     /transl_table=11
                     /translation="MMMIIINKLLIINNNFLGVVVIGKLKKYFKAFYQDDFENEFLQDD
                     RTVIAIDTNYLLSILRLDPEVANKYIDALEKNKERIYIPYFVGLEFSFNKINVKIDQDN
                     KLKDIDNKINKLKNQLDSTSQEISKLTNSNKLDNMKKISNEYIEQFKGLIDDSSTIEEN
                     RQENYLKLIDIIDDLLGEKPDQTFIDNIEKEGKLRYEEELPPGFDDSKKDGTRHFGSLT
                     YHQKYGDLLIWREILKHFSSSKNSKRLIYITDDGISNKKNDLFYKYGGRTVGPHIHLID
                     EANEVANLDLYIIDSKSFVKYSNVSLNKSDESQINNLLNTRYVSNAGYMSVDVRDFEDS
                     TDDNILEDSQSYHIRNKIDLCLDQHYLNSLFTNDLFGNRSSIDEVCTTILNISKKLCML
                     YKQCNDLDREYLISQYISFMLNIYSSIDDKEDKVLFIKSLKKCLTPLYFVSDLKHKDKL
                     SSLTDYLVEKSYKYYKLKSHDLKE"
     misc_feature    complement(17895..17910)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 23.97"
                     /tool="antismash"
     misc_feature    19446..19462
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.7"
                     /tool="antismash"
ORIGIN
        1 atggattttt ttgtatattt tattttatgg ctactatatg ttgtgttata cttagaacag
       61 gtacctatat acggcgccta ttatactaaa aaaaaccaag aatattggct tttttggagg
      121 attggttatc gaaaaataca ataataacca atctttattg ttttgttttc ctttatcttt
      181 tttgataacc atattcctct tagaaaggag gaatatcttc catgattgga attatgatta
      241 ttctaatctt ttttggatca atgcctttag ggctatgtca aatgattcaa tattacaaaa
      301 atctcaaggg ataattgagg taagttagag atgtgatagt tgcagctatc acatctcttt
      361 cttttcctat tgattattat attctgttaa tgtataattt acaacaagta cacacactat
      421 atatagcaga tttttaaact agtttaaaaa tctgctatat atagtttaat aataactaat
      481 tagttagaat tatattacta ttcggtatga aatatttttc tatacaatat ataatatgcg
      541 agttttaata ggagagatat aaatatgaca gaagacgata aagatatgac agaactatat
      601 gctaactgga gaaaagatca atatcatgaa aacaaaggat tttttcctat ttttcaatct
      661 ttttcaagta aaatgacaaa actatcaaat ggggcaattg ctttatatgt atttttggga
      721 ctcaaatcaa actacaaaac tggtgtttct ttttatagcg ttaaaaaatt aagcattatt
      781 ttcaataaat caccacgtac aattagtttt tggattaaag atttagaaga taataaactg
      841 atatatagaa aacaaaaaac attaaatgga gtaagcacaa cttacttact tccatactca
      901 gataaaaata aagatactaa tttttaaatg ataatttata ttaaatattc tatatctaaa
      961 tgaaagagga taattaaatg atatacgggt atgcaagagt tagtacttta gggcaatctc
     1021 tagataatca aatccaggat cttagggata atggtgtatt aaaaaagaat attttttctg
     1081 ataaatatac aggaactagt accgacagac ctggattcca aaatctatta gacactctaa
     1141 aacctgatga tactttggtc gttactaagc tagatagatt agctagaaac actagagagg
     1201 ctcttaatgt aattaacgag ttgcaagata aaaatattaa aatcaacatt ttaaaccttg
     1261 gaattgtcga taattcatca ataggaaaat ttacgacaac cgttttatta gctgttgctg
     1321 aaatggaaag agatatgatt gtaaatcgaa cccaagaagg taaaaaatat gctaaaaagc
     1381 ataatcctaa ttatcgagag gggcgtagga cacgcctcac aggaaagaat aaggacaagt
     1441 acccagctat ttatgaatat aagcagtctc acacggctga ggagaccgca aaagcatttg
     1501 gaacatctgt taggacagtt ttttatattc aatctatctt taaaaagcag aagtaagtac
     1561 tcaagtaatc caatcgtaag cttcttccaa aaattgttct aaagataaaa taaaaaaaca
     1621 ctcaattgag tgttttttga ttaataatgg tatactcgtt ttccattaag aatctcaaat
     1681 ttaggttgga tactcatatc ttgataccaa gttagataca atctagcgtc aatgctgcca
     1741 ccgcttgtat ctaaatcaac gtggtctata acaatactaa catcgctatg atcttcaaga
     1801 taagtcatta gcgttccatt tagtagtcca ttgaagagaa tttttgccat tgctttgtca
     1861 gaatttacaa agtttccaga tgtaactaca aattcgcttc catatccgtt actatcaacg
     1921 ataggttcaa ccattttacc agttgttaca ccatatattt ttccgtattg tgttaggtat
     1981 ggatcaagta gtttttttaa ttctgcttta atattttcct tgttaggtac atattgacct
     2041 ttcttatggc catctgaatg gggtaatttt aattttggtt taactttagt gtttgttttt
     2101 ttctttatat taggaatggt taccgttttt ttatgggtat taactacttt attatgcttg
     2161 gcagctttta cttttacctt tttaacatac tttgtattaa cccatccatt cctaccatta
     2221 cttgctaaaa catatttata aatcaacctc ttaccatgac gttttacaac aatcgttttc
     2281 ttaacaatca acttgtgtga accaaagtaa tatttagctg aatgtgattt aatggatttg
     2341 atgtgtgcat ttttgtaaac atatccgctt tttttgattt ggtaatgaaa agatgaagct
     2401 gatgcaatta cattcctttc aattggtgtt gcaagcaatg cggttaatag cgtcattgct
     2461 cctattattt taatatttac tttgcatagt ttagagtggt ttttcatgaa ttattcacct
     2521 aaataatcga ataagagaat ttttgtttta tacatatttt aaaattcact atcatcttga
     2581 ttttcaacca tttctcgaat gaaatgataa aatttctccg cttcattggc actaattaaa
     2641 ttcttcttag ccttatcttc actacctgca tttctgataa ctgtaaagat ataaaagtcg
     2701 atgattttag atatcatttc accacgtttt aacgtgatat gagttgatga agtattaaag
     2761 ttaatgacat gatcaagctt ctctgcaatc tcttttttta gtttcatcga aacaaaacct
     2821 ttgtcatatt cctttttact ctttttcttt tcatcatctt tttgaaaaat tggtgtatct
     2881 tgattagatt ctagtatgta atttgcactg tctagatcta caaaattttc atttgaatca
     2941 ttatatttac gaccgacttc ttctaatttc tttccgcttt ctcgatttct ttttatcatg
     3001 tcttgtacac ttttcgaaat ctcgtctgac ataatttcac ctcaaaaata atataatttt
     3061 tgatatttta ctcctcaagg aatataaaaa tcaacctaaa agctccttaa ggagtacact
     3121 ttagcaactg tcttaatagc tccttaagga gtttttaggt atgcgttaag gagtacccag
     3181 ctatttttat gtaatcatat gtgagtacct gtattgataa atcaatgttc tcatgggaat
     3241 ataaaatccc aactaaaaac cccgtcgcga cgggaggata cgcttgtcgt tccccagttt
     3301 cccttatcct gtttaaaaaa taactcttaa ttaaaacaaa ctactgctat ggttaaagaa
     3361 ataggtgcag caatgcatac aaaaattaag cggttaagtc ccttattcac tgattcaatg
     3421 tcatcatcag atatatctct tttaaactta taaaatctag aaaacgatcc agctaaaaac
     3481 ataagacaaa gaaacatcat tccttttttg taatcagagt ctgaaaacca aagtaaaact
     3541 gacatataaa tgtaagatac aaattcaagt ccaagaacaa ggttattttt tttattaaga
     3601 ttcataactt aatcgactcc ttaattaatt agttattaat ataaaatatt taaaacacta
     3661 acagccatct atggatcaac tatctttttt aggtcagttt ttataaataa tacaaaacta
     3721 tacggataca ttattaaata catccatatt gtaaaaagca agaaaagaaa taactatcaa
     3781 tctaactagt tcgttctttt tatatttatg gtttatttgg tagtattata ctactaatag
     3841 aataaataaa gtgaaggtaa tatatatgga taatttaaag gttttaggta gaatgtcggg
     3901 ttttccttat aaacatgaga aaatagtagc agttctagaa agtaaaaaag atacctttga
     3961 gaatacgcgc tatacagttt ttagcacggg gacgcatata aaagttttgg aaatatctga
     4021 tcgtataact tctcaaagaa acatatcttt tagcgagttt attgaaaagt gccatataga
     4081 tgaacggtcc gtgagagaat ttaatgatga atatggtatc cgcttaactg ataatataac
     4141 atttgatgaa attcgttttg gagtccatct tttagaggca atttatgacg atgattatac
     4201 taaaagttta gttggtaaaa aactatttat tagatcttta gtatcagatc attacaccgt
     4261 taaggctgca gaatcgttgg aatatggcaa aattttaaag gtaacatatg ataacggcga
     4321 tcatgatttt ttcattaatg aactaccagt ttttaaagtt gatatgtata caccatattt
     4381 tgctaataaa ttaagtaaag caacgtctcc agatacggca tatatgatcg tgtttaattc
     4441 ccgatatcat ttaaagaata gacagggttt aatcgatgat tatttagaaa aaattcctaa
     4501 aaacaaacaa caattaacgg ttataaatag gctaaaatca gaaagttttg aaaagctatg
     4561 tttggaacga ttaaaagaca attcaagaat atctaattac aagattttta aaatggacga
     4621 tcaagatttt gttttttaca aaattagcgc tgtaagatat tactaatacg tattgataaa
     4681 cctaaattct ttatctagga attttaagta ggaatatccg ggtatttttt ccctctattc
     4741 atacactgtc gccggtgtta attatacatt tatagaaatg tccccatgaa tttatatcct
     4801 aattcaagtc ttatatagga tacttaatct aacgcaccta taaatacaat atcataggca
     4861 acataagata agttagttac actattttaa tacaactaac aatgtttttg ttttttaaaa
     4921 attaagtaat tgttactatt tctcattcta aaaaactata ttctcaggaa atagtctttc
     4981 tatatcattg ttataatgta tatgttgtga ataattatct attgcttctt caaataaatt
     5041 tttgaacgat ttaatcatat atccatatct attcttagct tttctatttg cattacgaat
     5101 aattggttca gtatgatttt ctaaatattc atttaataat ggattggttt caaatctacg
     5161 caaactacta tttttagcta cactaaatct taaggccata cgcttagctt ttaaaataag
     5221 ctttccgtaa tcgtataatg tatcaggttt attaaaagat agggctttta attgtgatac
     5281 taaaagtgga ctgaatccag ctttattgat tagtgattgt gcaatgccat ctaatttaag
     5341 agattcaaca gattttttag tgtcattttt attgtgtgat atctttttat tagtattatt
     5401 atattcagtc ttattagaaa ccaatttttg tgttttagta gtacaatttt ttgtttttag
     5461 tgagatgggt aaaaaaatac gattggttga attaaatctt tgcttaacaa tcaaccaccc
     5521 ttttctcttt aatgaattaa aaatgtttgt aacagtgtta gttgatacgt ttaatttgtc
     5581 ggccatatct tctcttgaat atgtaacgta atatgcattt tgttgcttgt cgtaaaagtc
     5641 actgtttcct tcggataatg acatgcgatc atacaacctt gtgagtgcaa cttgttctgc
     5701 tggggacaaa ccagcgtctg aaaattcttc gaatagtttg gaaaacttca taataagagc
     5761 ccctttaata ttgattttta aggggttaag ggctataata tgtacataaa tcatacaaat
     5821 tagttattac attacagctc ttaaccaggt tgttgtaatt ctcaaaagtc ggttgttcca
     5881 tcagccggct tttttgttta catgaataat actaacattc ttttaatcat tgtcaaacga
     5941 atatttttag tactttttat aaattagcat gacagcatga tacaattaca taattatggc
     6001 atgacgtaat aaaagcacga tgtaattatg taattgcata ataaaaacat aaaagcataa
     6061 aaacctgatg taataaaagc atgatagcat gacgtataaa aagctgttaa atagcaattg
     6121 ttttcagcat gatgtaatta aagcatgata gtgttataac ataatgtaat taaagcatga
     6181 tagcattatg tcagaataat gttatttcat gctagcatga aataacaatt gcattatagc
     6241 atgacagcat gatataattc taaatagaga gagggtataa atatgacgag aattattacc
     6301 ttaggtaact tcaaaggtgg agttggtaaa acaacttctg ccagtcttat tgcttacaat
     6361 ttatctgacg aaggatataa agtattactt ggtgatttag atccacaagg taatgccact
     6421 aatatgatgt taaaaaccaa agcaaacttt gacggcaagg ttagttcatt tagacaaaca
     6481 ttgatgaagt cagtagaaga agaagattta agcaaatcga ttatcactat tactgacaaa
     6541 ttagatttgt tagcgtctgc tccagatttc gcgctatatc ctagatacat ggaaaagata
     6601 gctgattaca ataaacgagt tcaatattta aaaacactat ttgataaaat taaagataac
     6661 tacgattaca ttattttaga tactccacca actatcatgt cgctattctc agacagcgca
     6721 ctatacatgt cagattggtg cttaatcgtt atgcaaaccc acaaagatag ctttgacggt
     6781 tcaaaggcct ttattgatta tctgcaagaa tatgtggttg atgcatacaa agcacctaaa
     6841 cttgatttag ttggtatctt gccagttcta ataaagaaca atgctccagt ggatgaatac
     6901 actttgcaat cagctaagga ccaattcggt gctgaaaaca ttcttgatca tcacattaaa
     6961 cacatggaaa gaataaagcg attcccaatc actggaattg cagatcatga attatgggac
     7021 aagagagtaa ttcaagcata taaagaagta actgacgaaa tgctagaccg cattagaaag
     7081 gaagaggctg aatgagttca ctaattaatt ccaaccgtaa agacgatgtt aaaccattaa
     7141 agagtgcgac tttagctgac cttgaacagg aaaaatctga tgaacaacca aaaaaagaag
     7201 ttaaaaaaga accagcttac aagggacaca aaacaacgct aagcattgat agtcacacca
     7261 agaacatgtt acaagttatg gttttactag gagacgctcc aaaccaaaaa gaagggttct
     7321 cagtagcaat taacgcttac tacgacaatt taactcaaga taaaaagaat atgtttgata
     7381 tgatgcttaa gcaattaaac agccatgatt aattcaaata cagtcataat ttaatacaac
     7441 taaaaagggc actattataa tagtgccctt tttagtttct tctatgatta ctaagatagt
     7501 aaaaatatga tagatgtgtt ttagacaact gacccacatc acataactat tggtaataaa
     7561 taattgaaaa tttgaatata atcataatat gtccacatat agtttcaata ttagttattg
     7621 tcaatgatta tatttccatt gactttttta ttatatgatt tgaaatattt taaaaatcta
     7681 gatttaatat ttttatagtt atggtgtctt ctttgtattg caatgtattc tgctagtaaa
     7741 gcagaaactt ttgctgatgc caacgaagtc ccataagcgt aggagtagct attttttatt
     7801 gtagcaacag taattaattt tttgctattt aaattgtgac tattccagta attggatcca
     7861 tatttaaagt attcagtggt gtctcctccc ggagcgaata gactaatgtt tttgcttcta
     7921 ttagaaaaat tagattcgtt tccggatgga cctagtgaac cgaccgatat aacattcttt
     7981 aataatgctg gtacatcaac taatttttta atttttttta cactgatgtt attatctttt
     8041 gaaaaacttt ttttgaatct atggatatta ttctcgtcat aaccattttc acccgcgcta
     8101 gcaacgatga tactaccatg tttctttgaa tattttatgg ttctattcca tgccttaaat
     8161 tccatatttt ttttcaaact tttagatgag tataaatatt ttccaaggct tacattaatt
     8221 acagaatcct tattgttagt ggcagaaatg attcctttca ttatccaaaa tgggtttgat
     8281 tgtttttttc caacaacacg gtatatatga agtccaactt taggatatac acctattact
     8341 ttaccatgtc cagaaatttg accagcaatc atggttccgt gtcctatctt atcgtaaatg
     8401 tatcctttat ttccatactc atcattctcc ttaccgttaa atccaccttt aggaaccagg
     8461 tttactgatc ctttttttat tatagttcca ttaagagagg attgattaga tgctattcct
     8521 gagtcaacca ctccaattgt tacggatggt gaatataata cgtgtttata catttttcct
     8581 ttaagcgtga tagccttgat atcccattgt ttattccaat attttggttt tttaaactta
     8641 tattctgggg tgtaattatt ttttctccaa atctttatat ttgtcttctt tttttctatt
     8701 gtcttatgat gaagtatatt atgtttatct ttatctttat ctttatctat cctgaatctt
     8761 acatagtaaa tttctggtat gtcatatatg atctttccct tcttcttttt gataagttga
     8821 agtacttttt tgtattgaat tcgggagtta gataatgact catatgttga ggacgcattt
     8881 gctttagcat ctacagaata tataagtatt aacacaaatg aaataacaaa tactatacat
     8941 aacttaatag gggaagatat ttttctatta ttcatttcat gattctcttt tcgtgattat
     9001 ttatatataa aatgcctgtt ggttataaca acaggcattt tgtgaggtaa gatatttttg
     9061 ttttaattca aagtttttta tttatctagc gtggaaagag aaatgattag agctgtgaca
     9121 atgacaagtc tttgtcttgt tattacagcc cattaaacgt cctgtaatac aaccaggtgt
     9181 acataaaaca tagcttgtaa ttcgtggttc aagacctttt ttacttccag aatggacttt
     9241 tttgatacct aagttaaaat cgttaaaatt agacatatct attacctcca tcaaatttat
     9301 cttaacctct taagatgaag tataaattaa acaggtacac tgttcaataa gattttaaag
     9361 gccaaaaatc ttaccccaat gaacatattg tttatctttt aaggatagca atgatagaaa
     9421 tattcctgaa gttccagata acattcctaa gtcaggtata ttgcgtgatg tttcagcatc
     9481 gtcaaacctt tcactgaaaa atccagtaat ggattcgttg tcgaaatagc ttaataataa
     9541 tttaactatt tttttattta aatgtatatc taatgagtaa tccttattca gactatttaa
     9601 aatagctaaa attcctgcat acccatgaca cattgaagga tcagctatac ccaaaggttt
     9661 tttatttatt tcttgtaatc ctcgtgacat taaatctact tcgtcaggat ttctttttat
     9721 caaattatac tttaataatg caatatcaat acccggggta ccataacacc aactatcaaa
     9781 tcgttctaca ctatcttttt tttctttggt aaaggaatta aaatctataa aaaaaggtgg
     9841 gcaaaaaaca tcgtttttaa ccaataaatt atcataaata aaggtatgca ttttattcat
     9901 taaagttagc tgattggaaa catataagcc actaaggtat gcattcgaga gactgtataa
     9961 taatcctgat attccatgag agaaacttat gttgaagttt ccctttggat aatttattga
    10021 ttctcttttg tccatttgat gatctgagga aacaaaccac gcaggaacag aaatatcatc
    10081 ataaattagt tgcgttgaat acatgtcaac tatgtgtttt tctattctta taatacgttt
    10141 ccctactata tctttttcat tctcatcatt ggaattatta aaaaagtata ctaggtaatt
    10201 taaaacacca gatagtccat acatagtatc gaaatcatat gaatatgcag gagaatgtat
    10261 tctatttttt tgttcagaaa tttttacgtc tattaatgtg aatagaaaat cattcagttg
    10321 gtttataacg ccacataagt ctggcctgtt taataaagct aaagaaaaag ctattcctga
    10381 tactccttca aacaaggatg catccattat cggttttttc tgtatactgt caagtaaaat
    10441 tgaaattaag tcatccgtat tttgtttatt gtgaataatg ttttgtttgt ttaattcttc
    10501 tgaaagtaat attagaccaa ttattccgtt ggatagatct tgtttaaaaa aatctatatt
    10561 gttatttgag ctttgttttg ctaaataatc aatgttttct cttataaagt ctatatttag
    10621 tattttttct gaatgttttt ttataaaatc aattgtttca tcattcatat ctttcagcct
    10681 cctctaattt ttggtattcg gtacaattat ttaacaagta ttcatgacta ccttcagcga
    10741 caatttcacc cttggacatt acaatgattc tatccgcatt tttaactgct cctattctat
    10801 gtgatatgaa taatcccatt ttattatgag atataaattt tttgaattca taaaataaat
    10861 tactttcaga gatggggtct aatgcagagt taggttcatc taatatcaca aaactagcat
    10921 ttctattaaa aaaggccctg cttgtagcaa tgctttgcca ttggccaccg gacagttgtt
    10981 taccgtttcc aaaccaattt ccaagagaat cttcaacttt caatttttta taaagattat
    11041 cattatacgt ctttagagta ttcataatct tatcttcttg tttaaggtta cgtatatcgc
    11101 caaaaccaat attttcacgt actgacaact catactgaac aaaattttga aataaaacgg
    11161 atattccttt atggaaagag tgcttattaa gcttattgga atcgatattg tttattaagt
    11221 attgccccct tgttggttta taaagtccag taagaatttt aacaatcgta cttttaccag
    11281 atccgttggc acctacaatt gcaacgattt ctcctttttt gattttaaag cttatattat
    11341 ctaaagcttt cacactattt ttatatgagt aagagacgtt tttaaattca atcgttgtta
    11401 ttggtaggtt ataataactt ccattatcat ctttttcatt atcatctata aaatcataaa
    11461 gtttacgaag aatttgcaaa tcatatttta aattaaataa attgttaata aatgtctgtg
    11521 tgcttccttg agtaagactt attatttgag tcacactggt taaggtcccc aatagagcct
    11581 tgcctagata aattgaccaa atcactgata gaacaagtat agctgttaca atactaacta
    11641 ttaagtcata tacataatta aatatagaga gattcttcaa aatttttaag tcctgtgata
    11701 taaattcttt ttttagattg gcaaactttt tctttaaatg ttttgataag tcaaagaaaa
    11761 ttatttcttt gacgctaaaa tcatgagtta atagatattt tatataccaa gccttacgtt
    11821 ctttttcggc tctattccaa tgaacattaa actgattgtt gcttaatatt agtaggaatg
    11881 gaatagaaat gaaagttaaa agtaccagtg ttatgccata tattgggttt attttgaaaa
    11941 tataaaaata actactaatt atcgtaatga tcgatacact aattgaattg aatgcggtaa
    12001 atacagaaaa aggttttgac ggggcttcat tagacagttt atctataact gaatatgttt
    12061 cttcgttttc aaaagctgtt atatccattc tagatactac atttaaaatg tcatattcta
    12121 cttcaaaaga caatctatta cttataattc cgtttatgta agactgtact gatgatatag
    12181 aatctgttat caacgaaact gagatgtatc ctgcaaaaca cattaaaatc aacgaaaaat
    12241 gagaacttaa tgataaaaaa ttgatgatta gttgtacaaa aaatatagaa attaaaggaa
    12301 gtattcctaa tacaatattt ataaaaatca ggatagaaaa taaaatttta tgttgcttcc
    12361 atataagttg tatcgtttgt ttaatctctt tcattgcttg tgattctcct aataactcaa
    12421 tattcttttt tagtaagatt atcagaaaat atagtgtatt tccaataaat ttattatgat
    12481 ataattatga gaaaaatgga ggtgctctta tggcaaatat tttagaaact aaaaacgtta
    12541 caaagagttt tagaaatcag ttggctttgg ataacgtttc tgttcatata ccaaaaggag
    12601 aagtttattg tttgttaggt cctaatggag caggtaagac aactttaatg aagataataa
    12661 caggaatgat tcataaaaca tcgggaaaag tgatctttga tggcaaagaa tgggaaagag
    12721 atgcattaaa cgatataggt tctttaattg agaatgctcc tatatatgga aatttaacag
    12781 ccttagaaaa tttaaaggtg gttgctactt taagaaaagt tccattaaat catcttgaat
    12841 ctattttaga acaggtagga ttggagaata acaaaaaaag gtctaaagat ttttctttag
    12901 gaatgaaaca aagattaggt attggaatgg ctatggtttc aaatccaaaa cttttaatat
    12961 tagatgagcc aacgaatggt ttagatccaa ttggaataca ggaactgaga actctcataa
    13021 aaggactggc aaaaaaagat attactattc taatttctag tcatgatttg tcggaggtag
    13081 aacacttagc agataatatt gggattatat ctggtggaaa actgttatta gaagagaagt
    13141 ataataatga aaaccttgaa aaactcttta atgaaatagt agggggagta tagttatgac
    13201 tagtttaatt aatagtgaat ttttaaaaat tcgtagaaca tcaatccctt ggctaactat
    13261 tctagtgcct tttttctgtg tgtttttagt agctgttgtt tttgcgggaa atgagtattc
    13321 tatgcaaagc attattaatc aatggagctt aatttgggta ctattattct ctgtattgtt
    13381 atctggaatg accactcatc acgaatcaat atctactcaa tataagtcaa tattaagttc
    13441 accagttagc ttatttaaat ttgaactttc aagattaatc atgttagtcg aattgttctt
    13501 tataggaaat gtatttttag ccatactagt atgtatttct aaattttatt ttagtagcat
    13561 ggtcggaatg ggctatatac tactatcaat aattatggtc tctatttcag gattatggac
    13621 agtgcctttt ttcttgctaa taagcagatt atcaaatatg tttgttggca tattaattgg
    13681 tttatttggt ttcttgatag ggggagtttt ttctggaact cgttttggat atttaatccc
    13741 atttacgtgg tcaccagaga cagcatctcc tattataaga atgcatgtta acggggttcc
    13801 gtacagttta ggtactcatt cttttatgaa tacaacgtac attatcttat cgttatcatt
    13861 attcattgta ttggcattaa ctgaatgtct actatttact cgtcaggagg ttaaaaaatg
    13921 attgctagag tttttaagtc agaagctatt aagcttatta atagccatat cttatatttc
    13981 cacatagctg ccatggttat ctttccaact ttattagcgg ttttttttgg taagaatcat
    14041 tacccgctaa caaattttgt gttgattccc aacttttttt taatcatttc ctttgtgggt
    14101 cctgtagtga tctcaatagt tgcatctatg gttaacgaca gagaaacaaa agccggcaat
    14161 tataagaatt tattagacac ttcaatcagc ccattaaaag tttttcaatc gcaaatagct
    14221 ttttattggt cactatatct gacagaaatc atattatcat tttatatata cggtttaatt
    14281 ttgaacattg tatttcatgc acccctattt ccttatttta cgttattagt tttttgtatt
    14341 ctattctctt tattagcagt aattcagtac gaattgtgtt ttttagcatc atatatattt
    14401 ggtaccgctg gatctttgat tttaggattt gcaggattga tattatctgc actaagtgaa
    14461 acggttttat tcgataatgt ttgggaattt attccatggt catggcaaat tagaagtatt
    14521 gctttttttc agaatggtac tactattaat gattctattc attatgcgtt cttatatatc
    14581 tttagtatat taataagtct cgtgtcgctg ttcgtatgta ataagatctt ttataggatt
    14641 ttaaataaaa attgaggtaa taattatgag aacatattac aacgttgaaa atcctgtcgt
    14701 atttagaaaa cacttttgca atgttgaagg tggagaaaaa tttttatatg atgaaagttt
    14761 gttagattat ttaagcaagt ttgaatcgtt taaagaacaa attagagtgt caaaccatga
    14821 attatataaa aattacatgg attacttaaa agatagatca agtttttcaa aaaaaagaac
    14881 tagacacctt gttgaatcaa taacaggata ctttctgaga ataaagtata gaaatactcc
    14941 ttttggtctc tttagtggtg ttggagttgt aaacaatgaa aaacatacta aatacactca
    15001 tctaaaaaaa atagttcttg gatataggtg gaaatatagt gtcataaaca tgttagaaaa
    15061 aaggtatcgg tcaaatgtta aatatgtatt tgaaaataat atgacagata taaatggtaa
    15121 tccgtttttt cttgatgttg gtaaagaaag taacattatt gatggtaaat attacaatga
    15181 cgaaataggc tttaaaattt ctaaaaacaa attaacggaa ctccttgaaa aatttttgag
    15241 taaaccaaga tgcacagatg acatttttaa tgttattaaa ggagaatata aaaatattga
    15301 tcctagttat ataaaagaat atatagtcaa tctggttgaa aaaaagatta ttaaatctac
    15361 tttacgcagt tctttatctc aaacggacga agagtggata gaaaacgtca aagatttttt
    15421 aaaagtagta aaagataatc agtctataca aaaatttgaa acattaaaat cattaataag
    15481 ctcttattca ataacaaacg tcggtgaagg aatatatctt tttgactcta tttccaaatg
    15541 catgaacgac atattcccaa cgcagaaaga tgatctaaat gtagatttat atttaaatga
    15601 tccgtcttta tcggttacac aggataatct gaatgattta aataaatgta tagatatgct
    15661 aactaaaata tctatacctt atgatgagtt acatgattat acttcattat ttcttgataa
    15721 atttggtatg gatgtagagg ttccattata cgaacttata gacaaaaact caggaatagg
    15781 ttttccaaaa tatgattcct ccgataatta ctctacgtct aaggaaacgt tggaatcaat
    15841 tggttcgttg ttagatgaaa aattgtacac cgctctaata aacaaaagac cattaaccat
    15901 tgatcaatca acagttaatg atgtacaaag aattttaaac ggttctgata gccacaagga
    15961 tatcattcca aaatctatgg atgtatgtct aacgcaagaa agtcatgata ataaaatatc
    16021 atttacaatt tcagaaatat ttggttctcc attaggaagt tcaattacag gtagatttag
    16081 actattagat gacttatatc aaaaaaacaa agatgtatca gatgattata taacctgtga
    16141 cataaacgca tttccaaatt tattaagttt atcaacttta acgcaaaatc gacatctgaa
    16201 tgaatacgaa ttgtcggttg gtactaatgg aaatcaacac aagaatcaaa tatcattaaa
    16261 tgatttagtt gtagggtatg aaaacgacac cttatatata aggtgttccc aaaataataa
    16321 aattgttaga tttgttgatt ataacatgct aaatggagac ttaaagaacc ctgttataaa
    16381 gctattactt gcaataagca atcagtccga tagatggcca tttaatttcc catggagttt
    16441 tggtcagcca acgagaacgg ttattcctga aataatttgt aataatataa tcgtaagtcc
    16501 attaacatgg aatattaata ctgctaacta tgacaatagc tttgaagatt tttatcgtga
    16561 attcatttgt aaaatagact ctcttcaagt tccaaaatta ttttactttg tagaatcaga
    16621 taataaaatt gtaagtgata gaaaaaaaga gtctatttac aatatttata aacatttaaa
    16681 aaaatctaat tctaaaaatg caaaattaca aaaatgtgaa tttggcagct taaatgatga
    16741 cggaaaaact caagaaatag tagttacttt aaaaaacaaa tataaaaaca ataaccccat
    16801 taaccagaag ccaatatata gatcttgtga ctatttaaaa cctttgaatg tagttaatgg
    16861 atggttttat gtagaggttg agtttggtga cgttgggata aaaaatgaat ttataataca
    16921 agagttatca aaattaaatg atgatttaaa taatttgtat gatttattct tttttataca
    16981 atacgaaaac aaagacaatc caactattcg tttgagatgg aaaagtaatg atatctttat
    17041 tgtgcagaat cgtgtaacga agtatctaga ctcgttgatt aaagaaggta ttattaaaaa
    17101 ttatactaat aataactact ttagagagtt agatagatat ggtggaaaac aaaatattga
    17161 caaagctgag caagcttttc attatgatac acttttttca attgtttctt tccaaaacaa
    17221 tccggaaaag attgaaaaag aatcactagc catatactct tgcgtatcga tgctcctatc
    17281 catttctaat tccatagata caataattga tttcttaaac atatatgatt atccatataa
    17341 tattaataaa aatgcatctg gttggataag gaaaaacgaa aaatatatgg atgaatattt
    17401 aaacaattta acaatcgaaa ccaaatcatt gttagataga cgtaataatg aaataaacga
    17461 cattattagc aatagcgatc gaaatcaagt aaacattgta agtagctttt tgcatatgtc
    17521 tataaataga attttaggca ctgatagata tggtgaacaa caaataatgg gtatattact
    17581 aaagctattt aataaagcca aatacacaaa tgaaattacg ggttgcaaat tatatacaaa
    17641 caatagcgaa agttaattaa cttaacccga tttgctaact attaatggat aatgagtgca
    17701 ttgctatcta cattattact atgattcatc tattcaatga atcataaaag aggttacaag
    17761 ttttttgtca tccgagtgaa gatgcttata ttcataaata ggtggggaaa gtggtcatca
    17821 ttattgtgta ataaaaaatt taaataatac tactttcaat agaataaaat tattatcaca
    17881 taaatgtatg gtatgatgat gattattatt aataaattac ttataattaa taataatttt
    17941 ttaggagtgg ttgtaatcgg aaaattaaaa aaatatttca aagcattcta tcaagatgat
    18001 ttcgagaatg aattcttaca agatgataga acagttatag caatagatac aaattacttg
    18061 ttatctatat tgagattgga cccagaggtt gcaaataaat atattgatgc gttggaaaaa
    18121 aataaggaaa gaatatatat accatacttt gttggtttag aattttcttt taataaaatc
    18181 aacgtgaaaa ttgatcaaga taacaaatta aaggatatag ataataaaat aaacaagtta
    18241 aaaaaccaat tagattccac gtcacaagaa atttcaaaat taactaatag caataaatta
    18301 gataatatga aaaagatttc taatgagtat attgaacaat ttaaagggct tatagatgat
    18361 agttctacga tagaagaaaa tcgtcaagaa aattatttaa agttgataga tattattgac
    18421 gatttattgg gagaaaaacc tgaccagact tttattgaca acattgagaa agaaggtaag
    18481 ttgagatacg aagaagaact tcccccagga tttgatgatt ctaaaaaaga tggaactaga
    18541 cattttggtt ctttaacata tcatcaaaaa tatggagatc tcttgatatg gagagaaatt
    18601 ctaaagcatt tttcttctag caagaatagt aaacgactaa tatatataac tgatgatggt
    18661 atatctaata agaagaacga tttattttat aagtatgggg gcagaactgt cggtccacat
    18721 atacacttaa tagatgaagc taatgaagtg gctaatctag atttatatat aattgatagt
    18781 aaatcctttg ttaaatattc taacgtttct ttaaataaat ctgatgaaag tcaaataaat
    18841 aatttattga ataccagata cgtgagtaat gccggataca tgagtgttga tgtaagggac
    18901 tttgaagatt ctacagatga taatattcta gaagattctc aaagttatca tatccgcaac
    18961 aaaatagatt tgtgcttgga tcaacactat ttaaatagct tatttactaa tgatttattt
    19021 ggtaatagga gtagtattga tgaggtttgt acaacaattt taaatatttc taaaaaatta
    19081 tgcatgttat ataaacaatg taatgattta gacagagagt atcttatttc tcagtatatt
    19141 agtttcatgc taaacattta ttccagtatt gatgataaag aagataaagt cttatttatt
    19201 aaaagtctaa aaaaatgttt aacaccttta tatttcgtta gtgatttaaa acacaaggat
    19261 aagctatcat cactaacaga ctatcttgtg gaaaaatcat ataaatatta caaacttaag
    19321 tctcatgact taaaagaata gacaaccacc cattaacata aattcgaaat ttaactgttt
    19381 ttatgcaaaa atattaatac tttattgcat atgaggagaa tgttaataca aagtcactta
    19441 tactatgcaa aaaatatatg caaaaaatat acaaaaaaat ccat
//
