LOCUS       NZ_AP019008            19498 bp    DNA     circular CON 23-AUG-2024
DEFINITION  Apilactobacillus kunkeei strain FF30-6 plasmid pKUNFF30-6, complete
            sequence.
ACCESSION   NZ_AP019008
VERSION     NZ_AP019008.1
KEYWORDS    RefSeq.
SOURCE      Apilactobacillus kunkeei
  ORGANISM  Apilactobacillus kunkeei
            Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
            Apilactobacillus.
REFERENCE   1
  AUTHORS   Zendo,T., Ohashi,C., Maeno,S., Piao,X., Salminen,S., Sonomoto,K. and
            Endo,A.
  TITLE     Kunkecin A, a New Nisin Variant Bacteriocin Produced by the
            Fructophilic Lactic Acid Bacterium, Apilactobacillus kunkeei FF30-6
            Isolated from Honey bees
  JOURNAL   Front Microbiol (2020) In press
REFERENCE   2
  AUTHORS   Maeno,S., Tanizawa,Y., Kanesaki,Y., Kubota,E., Kumar,H., Dicks,L.,
            Salminen,S., Nakagawa,J., Arita,M. and Endo,A.
  TITLE     Genomic characterization of a fructophilic bee symbiont
            Lactobacillus kunkeei reveals its niche-specific adaptation
  JOURNAL   Syst Appl Microbiol 39 (8), 516-526 (2016)
   PUBMED   27776911
  REMARK    DOI:10.1016/j.syapm.2016.09.006
REFERENCE   3  (bases 1 to 19498)
  AUTHORS   Endo,A., Tanizawa,Y., Maeno,S., Arita,M. and Seppo,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2018) Contact:Akihito Endo Tokyo University of
            Agliculture, Department of Food and cosmetic science; yasaka 196,
            Abashiri, Hokkaido 099-2493, Japan
REFERENCE   4  (bases 1 to 19498)
  AUTHORS   Endo,A., Tanizawa,Y., Maeno,S., Arita,M. and Seppo,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2016) Contact:Akihito Endo Tokyo University of
            Agriculture, Department of food and cosmetic science; yasaka 196,
            Abashiri, Hokkaido 099-2493, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 0.7
            Genome Coverage                   :: 123X
            Sequencing Technology             :: Illumina Genome Analyzer II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001949975.2-RS_2024_08_23
            Annotation Date                   :: 08/23/2024 20:17:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,533
            CDSs (total)                      :: 1,465
            Genes (coding)                    :: 1,459
            CDSs (with protein)               :: 1,459
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:23:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 19498
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP019008.1.
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Annotated at DFAST https://dfast.nig.ac.jp/
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Annotated at D-FAST http://dfast.nig.ac.jp
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19498
                     /db_xref="taxon:148814"
                     /mol_type="genomic DNA"
                     /organism="Apilactobacillus kunkeei"
                     /plasmid="pKUNFF30-6"
                     /strain="FF30-6"
     region          1..19498
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..19498
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..19498
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[9355:17657]"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      9356..17657
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     gene            566..928
                     /locus_tag="FF306_RS00005"
                     /old_locus_tag="FF306_p00001"
     CDS             566..928
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025107.5"
                     /locus_tag="FF306_RS00005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00001"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_094750990.1"
                     /transl_table=11
                     /translation="MTEDDKDMTELYANWRKDQYHENKGFFPIFQSFSSKMTKLSNGAI
                     ALYVFLGLKSNYKTGVSFYSVKKLSIIFNKSPRTISFWIKDLEDNKLIYRKQKTLNGVS
                     TTYLLPYSDKNKDTNF"
     gene            979..1557
                     /locus_tag="FF306_RS00010"
                     /old_locus_tag="FF306_p00002"
     CDS             979..1557
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292173.1"
                     /locus_tag="FF306_RS00010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00002"
                     /product="recombinase family protein"
                     /protein_id="WP_094750991.1"
                     /transl_table=11
                     /translation="MIYGYARVSTLGQSLDNQIQDLRDNGVLKKNIFSDKYTGTSTDRP
                     GFQNLLDTLKPDDTLVVTKLDRLARNTREALNVINELQDKNIKINILNLGIVDNSSIGK
                     FTTTVLLAVAEMERDMIVNRTQEGKKYAKKHNPNYREGRRTRLTGKNKDKYPAIYEYKQ
                     SHTAEETAKAFGTSVRTVFYIQSIFKKQK"
     gene            complement(1642..2508)
                     /locus_tag="FF306_RS07675"
                     /old_locus_tag="FF306_p00003"
     CDS             complement(1642..2508)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FF306_RS07675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FF306_p00003"
                     /product="hypothetical protein"
                     /protein_id="WP_094750992.1"
                     /transl_table=11
                     /translation="MKNHSKLCKVNIKIIGAMTLLTALLATPIERNVIASASSFHYQIK
                     KSGYVYKNAHIKSIKSHSAKYYFGSHKLIVKKTIVVKRHGKRLIYKYVLASNGRNGWVN
                     TKYVKKVKVKAAKHNKVVNTHKKTVTIPNIKKKTNTKVKPKLKLPHSDGHKKGQYVPNK
                     ENIKAELKKLLDPYLTQYGKIYGVTTGKMVEPIVDSNGYGSEFVVTSGNFVNSDKAMAK
                     ILFNGLLNGTLMTYLEDHSDVSIVIDHVDLDTSGGSIDARLYLTWYQDMSIQPKFEILN
                     GKRVYHY"
     misc_feature    complement(2537..2550)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.07"
                     /tool="antismash"
     gene            complement(2560..3033)
                     /locus_tag="FF306_RS00025"
                     /old_locus_tag="FF306_p00004"
     CDS             complement(2560..3033)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FF306_RS00025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FF306_p00004"
                     /product="hypothetical protein"
                     /protein_id="WP_094750993.1"
                     /transl_table=11
                     /translation="MSDEISKSVQDMIKRNRESGKKLEEVGRKYNDSNENFVDLDSANY
                     ILESNQDTPIFQKDDEKKKSKKEYDKGFVSMKLKKEIAEKLDHVINFNTSSTHITLKRG
                     EMISKIIDFYIFTVIRNAGSEDKAKKNLISANEAEKFYHFIREMVENQDDSEF"
     gene            complement(3328..3606)
                     /locus_tag="FF306_RS00030"
                     /old_locus_tag="FF306_p00005"
     CDS             complement(3328..3606)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FF306_RS00030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FF306_p00005"
                     /product="hypothetical protein"
                     /protein_id="WP_094750994.1"
                     /transl_table=11
                     /translation="MNLNKKNNLVLGLEFVSYIYMSVLLWFSDSDYKKGMMFLCLMFLA
                     GSFSRFYKFKRDISDDDIESVNKGLNRLIFVCIAAPISLTIAVVCFN"
     gene            3867..4667
                     /locus_tag="FF306_RS00035"
                     /old_locus_tag="FF306_p00006"
     CDS             3867..4667
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FF306_RS00035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FF306_p00006"
                     /product="hypothetical protein"
                     /protein_id="WP_094750995.1"
                     /transl_table=11
                     /translation="MDNLKVLGRMSGFPYKHEKIVAVLESKKDTFENTRYTVFSTGTHI
                     KVLEISDRITSQRNISFSEFIEKCHIDERSVREFNDEYGIRLTDNITFDEIRFGVHLLE
                     AIYDDDYTKSLVGKKLFIRSLVSDHYTVKAAESLEYGKILKVTYDNGDHDFFINELPVF
                     KVDMYTPYFANKLSKATSPDTAYMIVFNSRYHLKNRQGLIDDYLEKIPKNKQQLTVINR
                     LKSESFEKLCLERLKDNSRISNYKIFKMDDQDFVFYKISAVRYY"
     gene            complement(4949..5815)
                     /locus_tag="FF306_RS00040"
                     /old_locus_tag="FF306_p00007"
     CDS             complement(4949..5815)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036540.5"
                     /locus_tag="FF306_RS00040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00007"
                     /product="hypothetical protein"
                     /protein_id="WP_188595232.1"
                     /transl_table=11
                     /translation="MIYVHIIALNPLKINIKGALIMKFSKLFEEFSDAGLSPAEQVALT
                     RLYDRMSLSEGNSDFYDKQQNAYYVTYSREDMADKLNVSTNTVTNIFNSLKRKGWLIVK
                     QRFNSTNRIFLPISLKTKNCTTKTQKLVSNKTEYNNTNKKISHNKNDTKKSVESLKLDG
                     IAQSLINKAGFSPLLVSQLKALSFNKPDTLYDYGKLILKAKRMALRFSVAKNSSLRRFE
                     TNPLLNEYLENHTEPIIRNANRKAKNRYGYMIKSFKNLFEEAIDNYSQHIHYNNDIERL
                     FPENIVF"
     gene            6284..7096
                     /locus_tag="FF306_RS00045"
                     /old_locus_tag="FF306_p00008"
     CDS             6284..7096
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807713.1"
                     /locus_tag="FF306_RS00045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00008"
                     /product="ParA family protein"
                     /protein_id="WP_094750997.1"
                     /transl_table=11
                     /translation="MTRIITLGNFKGGVGKTTSASLIAYNLSDEGYKVLLGDLDPQGNA
                     TNMMLKTKANFDGKVSSFRQTLMKSVEEEDLSKSIITITDKLDLLASAPDFALYPRYME
                     KIADYNKRVQYLKTLFDKIKDNYDYIILDTPPTIMSLFSDSALYMSDWCLIVMQTHKDS
                     FDGSKAFIDYLQEYVVDAYKAPKLDLVGILPVLIKNNAPVDEYTLQSAKDQFGAENILD
                     HHIKHMERIKRFPITGIADHELWDKRVIQAYKEVTDEMLDRIRKEEAE"
     gene            7093..7413
                     /locus_tag="FF306_RS00050"
                     /old_locus_tag="FF306_p00009"
     CDS             7093..7413
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036624.5"
                     /locus_tag="FF306_RS00050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00009"
                     /product="DUF5388 domain-containing protein"
                     /protein_id="WP_094750998.1"
                     /transl_table=11
                     /translation="MSSLINSNRKDDVKPLKSATLADLEQEKSDEQPKKEVKKEPAYKG
                     HKTTLSIDSHTKNMLQVMVLLGDAPNQKEGFSVAINAYYDNLTQDKKNMFDMMLKQLNS
                     HD"
     gene            complement(7612..8976)
                     /locus_tag="FF306_RS00055"
                     /old_locus_tag="FF306_p00010"
     CDS             complement(7612..8976)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.5"
                     /locus_tag="FF306_RS00055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00010"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_094750999.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.3e-34, bitscore:
                     110.0, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNRKISSPIKLCIVFVISFVLILIYSVDAKANASSTYESLSNSR
                     IQYKKVLQLIKKKKGKIIYDIPEIYYVRFRIDKDKDKDKHNILHHKTIEKKKTNIKIWR
                     KNNYTPEYKFKKPKYWNKQWDIKAITLKGKMYKHVLYSPSVTIGVVDSGIASNQSSLNG
                     TIIKKGSVNLVPKGGFNGKENDEYGNKGYIYDKIGHGTMIAGQISGHGKVIGVYPKVGL
                     HIYRVVGKKQSNPFWIMKGIISATNNKDSVINVSLGKYLYSSKSLKKNMEFKAWNRTIK
                     YSKKHGSIIVASAGENGYDENNIHRFKKSFSKDNNISVKKIKKLVDVPALLKNVISVGS
                     LGPSGNESNFSNRSKNISLFAPGGDTTEYFKYGSNYWNSHNLNSKKLITVATIKNSYSY
                     AYGTSLASAKVSALLAEYIAIQRRHHNYKNIKSRFLKYFKSYNKKVNGNIIIDNN"
     gene            complement(9083..9292)
                     /locus_tag="FF306_RS00060"
                     /old_locus_tag="FF306_p00011"
     CDS             complement(9083..9292)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03731.1"
                     /locus_tag="FF306_RS00060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00011"
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="WP_371416534.1"
                     /sec_met_domain="TIGR03731 (E-value: 2.1e-17, bitscore:
                     53.2, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEVIDMSNFNDFNLGIKKVHSGSKKGLEPRITSYVLCTPGCITGR
                     LMGCNNKTKTCHCHSSNHFSFHAR"
     CDS_motif       complement(9203..9292)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEVIDMSNFNDFNLGIKKVHSGSKKGLEPR"
                     /locus_tag="FF306_RS00060_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(9083..9202)
                     /prepeptide="core"
                     /locus_tag="FF306_RS00060_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="9.10"
                     /molecular_weight="4164.8"
                     /monoisotopic_mass="4162.0"
                     /core_sequence="ITSYVLCTPGCITGRLMGCNNKTKTCHCHSSNHFSFHAR"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEVIDMSNFNDFNLGIKKVHSGSKKGLEPR"
                     /leader_location="[9202:9292](-)"
                     /alternative_weights="4182.8"
                     /alternative_weights="4200.8"
                     /alternative_weights="4218.8"
                     /alternative_weights="4236.9"
     gene            complement(9356..10669)
                     /locus_tag="FF306_RS00065"
                     /old_locus_tag="FF306_p00012"
     CDS             complement(9356..10669)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017000.5"
                     /locus_tag="FF306_RS00065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00012"
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_094751001.1"
                     /sec_met_domain="LANC_like (E-value: 1.9e-54, bitscore:
                     174.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDETIDFIKKHSEKILNIDFIRENIDYLAKQSSNNNIDFFKQDL
                     SNGIIGLILLSEELNKQNIIHNKQNTDDLISILLDSIQKKPIMDASLFEGVSGIAFSLA
                     LLNRPDLCGVINQLNDFLFTLIDVKISEQKNRIHSPAYSYDFDTMYGLSGVLNYLVYFF
                     NNSNDENEKDIVGKRIIRIEKHIVDMYSTQLIYDDISVPAWFVSSDHQMDKRESINYPK
                     GNFNISFSHGISGLLYSLSNAYLSGLYVSNQLTLMNKMHTFIYDNLLVKNDVFCPPFFI
                     DFNSFTKEKKDSVERFDSWCYGTPGIDIALLKYNLIKRNPDEVDLMSRGLQEINKKPLG
                     IADPSMCHGYAGILAILNSLNKDYSLDIHLNKKIVKLLLSYFDNESITGFFSERFDDAE
                     TSRNIPDLGMLSGTSGIFLSLLSLKDKQYVHWGKIFGL"
     gene            complement(10662..12395)
                     /locus_tag="FF306_RS00070"
                     /old_locus_tag="FF306_p00013"
     CDS             complement(10662..12395)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.5"
                     /locus_tag="FF306_RS00070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00013"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_094751002.1"
                     /transl_table=11
                     /translation="MKEIKQTIQLIWKQHKILFSILIFINIVLGILPLISIFFVQLIIN
                     FLSLSSHFSLILMCFAGYISVSLITDSISSVQSYINGIISNRLSFEVEYDILNVVSRMD
                     ITAFENEETYSVIDKLSNEAPSKPFSVFTAFNSISVSIITIISSYFYIFKINPIYGITL
                     VLLTFISIPFLLILSNNQFNVHWNRAEKERKAWYIKYLLTHDFSVKEIIFFDLSKHLKK
                     KFANLKKEFISQDLKILKNLSIFNYVYDLIVSIVTAILVLSVIWSIYLGKALLGTLTSV
                     TQIISLTQGSTQTFINNLFNLKYDLQILRKLYDFIDDNEKDDNGSYYNLPITTIEFKNV
                     SYSYKNSVKALDNISFKIKKGEIVAIVGANGSGKSTIVKILTGLYKPTRGQYLINNIDS
                     NKLNKHSFHKGISVLFQNFVQYELSVRENIGFGDIRNLKQEDKIMNTLKTYNDNLYKKL
                     KVEDSLGNWFGNGKQLSGGQWQSIATSRAFFNRNASFVILDEPNSALDPISESNLFYEF
                     KKFISHNKMGLFISHRIGAVKNADRIIVMSKGEIVAEGSHEYLLNNCTEYQKLEEAERY
                     E"
     gene            12511..13194
                     /locus_tag="FF306_RS00075"
                     /old_locus_tag="FF306_p00014"
     CDS             12511..13194
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003649446.1"
                     /locus_tag="FF306_RS00075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00014"
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="WP_094751003.1"
                     /transl_table=11
                     /translation="MANILETKNVTKSFRNQLALDNVSVHIPKGEVYCLLGPNGAGKTT
                     LMKIITGMIHKTSGKVIFDGKEWERDALNDIGSLIENAPIYGNLTALENLKVVATLRKV
                     PLNHLESILEQVGLENNKKRSKDFSLGMKQRLGIGMAMVSNPKLLILDEPTNGLDPIGI
                     QELRTLIKGLAKKDITILISSHDLSEVEHLADNIGIISGGKLLLEEKYNNENLEKLFNE
                     IVGGV"
     gene            13197..13922
                     /locus_tag="FF306_RS00080"
                     /old_locus_tag="FF306_p00015"
     CDS             13197..13922
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.5"
                     /locus_tag="FF306_RS00080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00015"
                     /product="ABC transporter permease"
                     /protein_id="WP_094751004.1"
                     /transl_table=11
                     /translation="MTSLINSEFLKIRRTSIPWLTILVPFFCVFLVAVVFAGNEYSMQS
                     IINQWSLIWVLLFSVLLSGMTTHHESISTQYKSILSSPVSLFKFELSRLIMLVELFFIG
                     NVFLAILVCISKFYFSSMVGMGYILLSIIMVSISGLWTVPFFLLISRLSNMFVGILIGL
                     FGFLIGGVFSGTRFGYLIPFTWSPETASPIIRMHVNGVPYSLGTHSFMNTTYIILSLSL
                     FIVLALTECLLFTRQEVKK"
     gene            13919..14656
                     /locus_tag="FF306_RS00085"
                     /old_locus_tag="FF306_p00016"
     CDS             13919..14656
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR03733.1"
                     /locus_tag="FF306_RS00085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00016"
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="WP_094751005.1"
                     /transl_table=11
                     /translation="MIARVFKSEAIKLINSHILYFHIAAMVIFPTLLAVFFGKNHYPLT
                     NFVLIPNFFLIISFVGPVVISIVASMVNDRETKAGNYKNLLDTSISPLKVFQSQIAFYW
                     SLYLTEIILSFYIYGLILNIVFHAPLFPYFTLLVFCILFSLLAVIQYELCFLASYIFGT
                     AGSLILGFAGLILSALSETVLFDNVWEFIPWSWQIRSIAFFQNGTTINDSIHYAFLYIF
                     SILISLVSLFVCNKIFYRILNKN"
     gene            14667..17657
                     /locus_tag="FF306_RS00090"
                     /old_locus_tag="FF306_p00017"
     CDS             14667..17657
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016615.5"
                     /locus_tag="FF306_RS00090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00017"
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_094751006.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.3e-74, bitscore:
                     240.8, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 5.2e-15, bitscore:
                     45.9, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTYYNVENPVVFRKHFCNVEGGEKFLYDESLLDYLSKFESFKEQ
                     IRVSNHELYKNYMDYLKDRSSFSKKRTRHLVESITGYFLRIKYRNTPFGLFSGVGVVNN
                     EKHTKYTHLKKIVLGYRWKYSVINMLEKRYRSNVKYVFENNMTDINGNPFFLDVGKESN
                     IIDGKYYNDEIGFKISKNKLTELLEKFLSKPRCTDDIFNVIKGEYKNIDPSYIKEYIVN
                     LVEKKIIKSTLRSSLSQTDEEWIENVKDFLKVVKDNQSIQKFETLKSLISSYSITNVGE
                     GIYLFDSISKCMNDIFPTQKDDLNVDLYLNDPSLSVTQDNLNDLNKCIDMLTKISIPYD
                     ELHDYTSLFLDKFGMDVEVPLYELIDKNSGIGFPKYDSSDNYSTSKETLESIGSLLDEK
                     LYTALINKRPLTIDQSTVNDVQRILNGSDSHKDIIPKSMDVCLTQESHDNKISFTISEI
                     FGSPLGSSITGRFRLLDDLYQKNKDVSDDYITCDINAFPNLLSLSTLTQNRHLNEYELS
                     VGTNGNQHKNQISLNDLVVGYENDTLYIRCSQNNKIVRFVDYNMLNGDLKNPVIKLLLA
                     ISNQSDRWPFNFPWSFGQPTRTVIPEIICNNIIVSPLTWNINTANYDNSFEDFYREFIC
                     KIDSLQVPKLFYFVESDNKIVSDRKKESIYNIYKHLKKSNSKNAKLQKCEFGSLNDDGK
                     TQEIVVTLKNKYKNNNPINQKPIYRSCDYLKPLNVVNGWFYVEVEFGDVGIKNEFIIQE
                     LSKLNDDLNNLYDLFFFIQYENKDNPTIRLRWKSNDIFIVQNRVTKYLDSLIKEGIIKN
                     YTNNNYFRELDRYGGKQNIDKAEQAFHYDTLFSIVSFQNNPEKIEKESLAIYSCVSMLL
                     SISNSIDTIIDFLNIYDYPYNINKNASGWIRKNEKYMDEYLNNLTIETKSLLDRRNNEI
                     NDIISNSDRNQVNIVSSFLHMSINRILGTDRYGEQQIMGILLKLFNKAKYTNEITGCKL
                     YTNNSES"
     gene            17894..19342
                     /locus_tag="FF306_RS00095"
                     /old_locus_tag="FF306_p00018"
     CDS             17894..19342
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037255.5"
                     /locus_tag="FF306_RS00095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FF306_p00018"
                     /product="PIN-like domain-containing protein"
                     /protein_id="WP_143441330.1"
                     /transl_table=11
                     /translation="MMMIIINKLLIINNNFLGVVVIGKLKKYFKAFYQDDFENEFLQDD
                     RTVIAIDTNYLLSILRLDPEVANKYIDALEKNKERIYIPYFVGLEFSFNKINVKIDQDN
                     KLKDIDNKINKLKNQLDSTSQEISKLTNSNKLDNMKKISNEYIEQFKGLIDDSSTIEEN
                     RQENYLKLIDIIDDLLGEKPDQTFIDNIEKEGKLRYEEELPPGFDDSKKDGTRHFGSLT
                     YHQKYGDLLIWREILKHFSSSKNSKRLIYITDDGISNKKNDLFYKYGGRTVGPHIHLID
                     EANEVANLDLYIIDSKSFVKYSNVSLNKSDESQINNLLNTRYVSNAGYMSVDVRDFEDS
                     TDDNILEDSQSYHIRNKIDLCLDQHYLNSLFTNDLFGNRSSIDEVCTTILNISKKLCML
                     YKQCNDLDREYLISQYISFMLNIYSSIDDKEDKVLFIKSLKKCLTPLYFVSDLKHKDKL
                     SSLTDYLVEKSYKYYKLKSHDLKE"
     misc_feature    complement(17896..17911)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 23.97"
                     /tool="antismash"
     misc_feature    19447..19463
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.7"
                     /tool="antismash"
ORIGIN
        1 gatggatttt tttgtatatt ttattttatg gctactatat gttgtgttat acttagaaca
       61 ggtacctata tacggcgcct attatactaa aaaaaaccaa gaatattggc ttttttggag
      121 gattggttat cgaaaaatac aataataacc aatctttatt gttttgtttt cctttatctt
      181 ttttgataac catattcctc ttagaaagga ggaatatctt ccatgattgg aattatgatt
      241 attctaatct tttttggatc aatgccttta gggctatgtc aaatgattca atattacaaa
      301 aatctcaagg gataattgag gtaagttaga gatgtgatag ttgcagctat cacatctctt
      361 tcttttccta ttgattatta tattctgtta atgtataatt tacaacaagt acacacacta
      421 tatatagcag atttttaaac tagtttaaaa atctgctata tatagtttaa taataactaa
      481 ttagttagaa ttatattact attcggtatg aaatattttt ctatacaata tataatatgc
      541 gagttttaat aggagagata taaatatgac agaagacgat aaagatatga cagaactata
      601 tgctaactgg agaaaagatc aatatcatga aaacaaagga ttttttccta tttttcaatc
      661 tttttcaagt aaaatgacaa aactatcaaa tggggcaatt gctttatatg tatttttggg
      721 actcaaatca aactacaaaa ctggtgtttc tttttatagc gttaaaaaat taagcattat
      781 tttcaataaa tcaccacgta caattagttt ttggattaaa gatttagaag ataataaact
      841 gatatataga aaacaaaaaa cattaaatgg agtaagcaca acttacttac ttccatactc
      901 agataaaaat aaagatacta atttttaaat gataatttat attaaatatt ctatatctaa
      961 atgaaagagg ataattaaat gatatacggg tatgcaagag ttagtacttt agggcaatct
     1021 ctagataatc aaatccagga tcttagggat aatggtgtat taaaaaagaa tattttttct
     1081 gataaatata caggaactag taccgacaga cctggattcc aaaatctatt agacactcta
     1141 aaacctgatg atactttggt cgttactaag ctagatagat tagctagaaa cactagagag
     1201 gctcttaatg taattaacga gttgcaagat aaaaatatta aaatcaacat tttaaacctt
     1261 ggaattgtcg ataattcatc aataggaaaa tttacgacaa ccgttttatt agctgttgct
     1321 gaaatggaaa gagatatgat tgtaaatcga acccaagaag gtaaaaaata tgctaaaaag
     1381 cataatccta attatcgaga ggggcgtagg acacgcctca caggaaagaa taaggacaag
     1441 tacccagcta tttatgaata taagcagtct cacacggctg aggagaccgc aaaagcattt
     1501 ggaacatctg ttaggacagt tttttatatt caatctatct ttaaaaagca gaagtaagta
     1561 ctcaagtaat ccaatcgtaa gcttcttcca aaaattgttc taaagataaa ataaaaaaac
     1621 actcaattga gtgttttttg attaataatg gtatactcgt tttccattaa gaatctcaaa
     1681 tttaggttgg atactcatat cttgatacca agttagatac aatctagcgt caatgctgcc
     1741 accgcttgta tctaaatcaa cgtggtctat aacaatacta acatcgctat gatcttcaag
     1801 ataagtcatt agcgttccat ttagtagtcc attgaagaga atttttgcca ttgctttgtc
     1861 agaatttaca aagtttccag atgtaactac aaattcgctt ccatatccgt tactatcaac
     1921 gataggttca accattttac cagttgttac accatatatt tttccgtatt gtgttaggta
     1981 tggatcaagt agttttttta attctgcttt aatattttcc ttgttaggta catattgacc
     2041 tttcttatgg ccatctgaat ggggtaattt taattttggt ttaactttag tgtttgtttt
     2101 tttctttata ttaggaatgg ttaccgtttt tttatgggta ttaactactt tattatgctt
     2161 ggcagctttt acttttacct ttttaacata ctttgtatta acccatccat tcctaccatt
     2221 acttgctaaa acatatttat aaatcaacct cttaccatga cgttttacaa caatcgtttt
     2281 cttaacaatc aacttgtgtg aaccaaagta atatttagct gaatgtgatt taatggattt
     2341 gatgtgtgca tttttgtaaa catatccgct ttttttgatt tggtaatgaa aagatgaagc
     2401 tgatgcaatt acattccttt caattggtgt tgcaagcaat gcggttaata gcgtcattgc
     2461 tcctattatt ttaatattta ctttgcatag tttagagtgg tttttcatga attattcacc
     2521 taaataatcg aataagagaa tttttgtttt atacatattt taaaattcac tatcatcttg
     2581 attttcaacc atttctcgaa tgaaatgata aaatttctcc gcttcattgg cactaattaa
     2641 attcttctta gccttatctt cactacctgc atttctgata actgtaaaga tataaaagtc
     2701 gatgatttta gatatcattt caccacgttt taacgtgata tgagttgatg aagtattaaa
     2761 gttaatgaca tgatcaagct tctctgcaat ctcttttttt agtttcatcg aaacaaaacc
     2821 tttgtcatat tcctttttac tctttttctt ttcatcatct ttttgaaaaa ttggtgtatc
     2881 ttgattagat tctagtatgt aatttgcact gtctagatct acaaaatttt catttgaatc
     2941 attatattta cgaccgactt cttctaattt ctttccgctt tctcgatttc tttttatcat
     3001 gtcttgtaca cttttcgaaa tctcgtctga cataatttca cctcaaaaat aatataattt
     3061 ttgatatttt actcctcaag gaatataaaa atcaacctaa aagctcctta aggagtacac
     3121 tttagcaact gtcttaatag ctccttaagg agtttttagg tatgcgttaa ggagtaccca
     3181 gctattttta tgtaatcata tgtgagtacc tgtattgata aatcaatgtt ctcatgggaa
     3241 tataaaatcc caactaaaaa ccccgtcgcg acgggaggat acgcttgtcg ttccccagtt
     3301 tcccttatcc tgtttaaaaa ataactctta attaaaacaa actactgcta tggttaaaga
     3361 aataggtgca gcaatgcata caaaaattaa gcggttaagt cccttattca ctgattcaat
     3421 gtcatcatca gatatatctc ttttaaactt ataaaatcta gaaaacgatc cagctaaaaa
     3481 cataagacaa agaaacatca ttcctttttt gtaatcagag tctgaaaacc aaagtaaaac
     3541 tgacatataa atgtaagata caaattcaag tccaagaaca aggttatttt ttttattaag
     3601 attcataact taatcgactc cttaattaat tagttattaa tataaaatat ttaaaacact
     3661 aacagccatc tatggatcaa ctatcttttt taggtcagtt tttataaata atacaaaact
     3721 atacggatac attattaaat acatccatat tgtaaaaagc aagaaaagaa ataactatca
     3781 atctaactag ttcgttcttt ttatatttat ggtttatttg gtagtattat actactaata
     3841 gaataaataa agtgaaggta atatatatgg ataatttaaa ggttttaggt agaatgtcgg
     3901 gttttcctta taaacatgag aaaatagtag cagttctaga aagtaaaaaa gatacctttg
     3961 agaatacgcg ctatacagtt tttagcacgg ggacgcatat aaaagttttg gaaatatctg
     4021 atcgtataac ttctcaaaga aacatatctt ttagcgagtt tattgaaaag tgccatatag
     4081 atgaacggtc cgtgagagaa tttaatgatg aatatggtat ccgcttaact gataatataa
     4141 catttgatga aattcgtttt ggagtccatc ttttagaggc aatttatgac gatgattata
     4201 ctaaaagttt agttggtaaa aaactattta ttagatcttt agtatcagat cattacaccg
     4261 ttaaggctgc agaatcgttg gaatatggca aaattttaaa ggtaacatat gataacggcg
     4321 atcatgattt tttcattaat gaactaccag tttttaaagt tgatatgtat acaccatatt
     4381 ttgctaataa attaagtaaa gcaacgtctc cagatacggc atatatgatc gtgtttaatt
     4441 cccgatatca tttaaagaat agacagggtt taatcgatga ttatttagaa aaaattccta
     4501 aaaacaaaca acaattaacg gttataaata ggctaaaatc agaaagtttt gaaaagctat
     4561 gtttggaacg attaaaagac aattcaagaa tatctaatta caagattttt aaaatggacg
     4621 atcaagattt tgttttttac aaaattagcg ctgtaagata ttactaatac gtattgataa
     4681 acctaaattc tttatctagg aattttaagt aggaatatcc gggtattttt tccctctatt
     4741 catacactgt cgccggtgtt aattatacat ttatagaaat gtccccatga atttatatcc
     4801 taattcaagt cttatatagg atacttaatc taacgcacct ataaatacaa tatcataggc
     4861 aacataagat aagttagtta cactatttta atacaactaa caatgttttt gttttttaaa
     4921 aattaagtaa ttgttactat ttctcattct aaaaaactat attctcagga aatagtcttt
     4981 ctatatcatt gttataatgt atatgttgtg aataattatc tattgcttct tcaaataaat
     5041 ttttgaacga tttaatcata tatccatatc tattcttagc ttttctattt gcattacgaa
     5101 taattggttc agtatgattt tctaaatatt catttaataa tggattggtt tcaaatctac
     5161 gcaaactact atttttagct acactaaatc ttaaggccat acgcttagct tttaaaataa
     5221 gctttccgta atcgtataat gtatcaggtt tattaaaaga tagggctttt aattgtgata
     5281 ctaaaagtgg actgaatcca gctttattga ttagtgattg tgcaatgcca tctaatttaa
     5341 gagattcaac agatttttta gtgtcatttt tattgtgtga tatcttttta ttagtattat
     5401 tatattcagt cttattagaa accaattttt gtgttttagt agtacaattt tttgttttta
     5461 gtgagatggg taaaaaaata cgattggttg aattaaatct ttgcttaaca atcaaccacc
     5521 cttttctctt taatgaatta aaaatgtttg taacagtgtt agttgatacg tttaatttgt
     5581 cggccatatc ttctcttgaa tatgtaacgt aatatgcatt ttgttgcttg tcgtaaaagt
     5641 cactgtttcc ttcggataat gacatgcgat catacaacct tgtgagtgca acttgttctg
     5701 ctggggacaa accagcgtct gaaaattctt cgaatagttt ggaaaacttc ataataagag
     5761 cccctttaat attgattttt aaggggttaa gggctataat atgtacataa atcatacaaa
     5821 ttagttatta cattacagct cttaaccagg ttgttgtaat tctcaaaagt cggttgttcc
     5881 atcagccggc ttttttgttt acatgaataa tactaacatt cttttaatca ttgtcaaacg
     5941 aatattttta gtacttttta taaattagca tgacagcatg atacaattac ataattatgg
     6001 catgacgtaa taaaagcacg atgtaattat gtaattgcat aataaaaaca taaaagcata
     6061 aaaacctgat gtaataaaag catgatagca tgacgtataa aaagctgtta aatagcaatt
     6121 gttttcagca tgatgtaatt aaagcatgat agtgttataa cataatgtaa ttaaagcatg
     6181 atagcattat gtcagaataa tgttatttca tgctagcatg aaataacaat tgcattatag
     6241 catgacagca tgatataatt ctaaatagag agagggtata aatatgacga gaattattac
     6301 cttaggtaac ttcaaaggtg gagttggtaa aacaacttct gccagtctta ttgcttacaa
     6361 tttatctgac gaaggatata aagtattact tggtgattta gatccacaag gtaatgccac
     6421 taatatgatg ttaaaaacca aagcaaactt tgacggcaag gttagttcat ttagacaaac
     6481 attgatgaag tcagtagaag aagaagattt aagcaaatcg attatcacta ttactgacaa
     6541 attagatttg ttagcgtctg ctccagattt cgcgctatat cctagataca tggaaaagat
     6601 agctgattac aataaacgag ttcaatattt aaaaacacta tttgataaaa ttaaagataa
     6661 ctacgattac attattttag atactccacc aactatcatg tcgctattct cagacagcgc
     6721 actatacatg tcagattggt gcttaatcgt tatgcaaacc cacaaagata gctttgacgg
     6781 ttcaaaggcc tttattgatt atctgcaaga atatgtggtt gatgcataca aagcacctaa
     6841 acttgattta gttggtatct tgccagttct aataaagaac aatgctccag tggatgaata
     6901 cactttgcaa tcagctaagg accaattcgg tgctgaaaac attcttgatc atcacattaa
     6961 acacatggaa agaataaagc gattcccaat cactggaatt gcagatcatg aattatggga
     7021 caagagagta attcaagcat ataaagaagt aactgacgaa atgctagacc gcattagaaa
     7081 ggaagaggct gaatgagttc actaattaat tccaaccgta aagacgatgt taaaccatta
     7141 aagagtgcga ctttagctga ccttgaacag gaaaaatctg atgaacaacc aaaaaaagaa
     7201 gttaaaaaag aaccagctta caagggacac aaaacaacgc taagcattga tagtcacacc
     7261 aagaacatgt tacaagttat ggttttacta ggagacgctc caaaccaaaa agaagggttc
     7321 tcagtagcaa ttaacgctta ctacgacaat ttaactcaag ataaaaagaa tatgtttgat
     7381 atgatgctta agcaattaaa cagccatgat taattcaaat acagtcataa tttaatacaa
     7441 ctaaaaaggg cactattata atagtgccct ttttagtttc ttctatgatt actaagatag
     7501 taaaaatatg atagatgtgt tttagacaac tgacccacat cacataacta ttggtaataa
     7561 ataattgaaa atttgaatat aatcataata tgtccacata tagtttcaat attagttatt
     7621 gtcaatgatt atatttccat tgactttttt attatatgat ttgaaatatt ttaaaaatct
     7681 agatttaata tttttatagt tatggtgtct tctttgtatt gcaatgtatt ctgctagtaa
     7741 agcagaaact tttgctgatg ccaacgaagt cccataagcg taggagtagc tattttttat
     7801 tgtagcaaca gtaattaatt ttttgctatt taaattgtga ctattccagt aattggatcc
     7861 atatttaaag tattcagtgg tgtctcctcc cggagcgaat agactaatgt ttttgcttct
     7921 attagaaaaa ttagattcgt ttccggatgg acctagtgaa ccgaccgata taacattctt
     7981 taataatgct ggtacatcaa ctaatttttt aatttttttt acactgatgt tattatcttt
     8041 tgaaaaactt tttttgaatc tatggatatt attctcgtca taaccatttt cacccgcgct
     8101 agcaacgatg atactaccat gtttctttga atattttatg gttctattcc atgccttaaa
     8161 ttccatattt tttttcaaac ttttagatga gtataaatat tttccaaggc ttacattaat
     8221 tacagaatcc ttattgttag tggcagaaat gattcctttc attatccaaa atgggtttga
     8281 ttgttttttt ccaacaacac ggtatatatg aagtccaact ttaggatata cacctattac
     8341 tttaccatgt ccagaaattt gaccagcaat catggttccg tgtcctatct tatcgtaaat
     8401 gtatccttta tttccatact catcattctc cttaccgtta aatccacctt taggaaccag
     8461 gtttactgat ccttttttta ttatagttcc attaagagag gattgattag atgctattcc
     8521 tgagtcaacc actccaattg ttacggatgg tgaatataat acgtgtttat acatttttcc
     8581 tttaagcgtg atagccttga tatcccattg tttattccaa tattttggtt ttttaaactt
     8641 atattctggg gtgtaattat tttttctcca aatctttata tttgtcttct ttttttctat
     8701 tgtcttatga tgaagtatat tatgtttatc tttatcttta tctttatcta tcctgaatct
     8761 tacatagtaa atttctggta tgtcatatat gatctttccc ttcttctttt tgataagttg
     8821 aagtactttt ttgtattgaa ttcgggagtt agataatgac tcatatgttg aggacgcatt
     8881 tgctttagca tctacagaat atataagtat taacacaaat gaaataacaa atactataca
     8941 taacttaata ggggaagata tttttctatt attcatttca tgattctctt ttcgtgatta
     9001 tttatatata aaatgcctgt tggttataac aacaggcatt ttgtgaggta agatattttt
     9061 gttttaattc aaagtttttt atttatctag cgtggaaaga gaaatgatta gagctgtgac
     9121 aatgacaagt ctttgtcttg ttattacagc ccattaaacg tcctgtaata caaccaggtg
     9181 tacataaaac atagcttgta attcgtggtt caagaccttt tttacttcca gaatggactt
     9241 ttttgatacc taagttaaaa tcgttaaaat tagacatatc tattacctcc atcaaattta
     9301 tcttaacctc ttaagatgaa gtataaatta aacaggtaca ctgttcaata agattttaaa
     9361 ggccaaaaat cttaccccaa tgaacatatt gtttatcttt taaggatagc aatgatagaa
     9421 atattcctga agttccagat aacattccta agtcaggtat attgcgtgat gtttcagcat
     9481 cgtcaaacct ttcactgaaa aatccagtaa tggattcgtt gtcgaaatag cttaataata
     9541 atttaactat ttttttattt aaatgtatat ctaatgagta atccttattc agactattta
     9601 aaatagctaa aattcctgca tacccatgac acattgaagg atcagctata cccaaaggtt
     9661 ttttatttat ttcttgtaat cctcgtgaca ttaaatctac ttcgtcagga tttcttttta
     9721 tcaaattata ctttaataat gcaatatcaa tacccggggt accataacac caactatcaa
     9781 atcgttctac actatctttt ttttctttgg taaaggaatt aaaatctata aaaaaaggtg
     9841 ggcaaaaaac atcgttttta accaataaat tatcataaat aaaggtatgc attttattca
     9901 ttaaagttag ctgattggaa acatataagc cactaaggta tgcattcgag agactgtata
     9961 ataatcctga tattccatga gagaaactta tgttgaagtt tccctttgga taatttattg
    10021 attctctttt gtccatttga tgatctgagg aaacaaacca cgcaggaaca gaaatatcat
    10081 cataaattag ttgcgttgaa tacatgtcaa ctatgtgttt ttctattctt ataatacgtt
    10141 tccctactat atctttttca ttctcatcat tggaattatt aaaaaagtat actaggtaat
    10201 ttaaaacacc agatagtcca tacatagtat cgaaatcata tgaatatgca ggagaatgta
    10261 ttctattttt ttgttcagaa atttttacgt ctattaatgt gaatagaaaa tcattcagtt
    10321 ggtttataac gccacataag tctggcctgt ttaataaagc taaagaaaaa gctattcctg
    10381 atactccttc aaacaaggat gcatccatta tcggtttttt ctgtatactg tcaagtaaaa
    10441 ttgaaattaa gtcatccgta ttttgtttat tgtgaataat gttttgtttg tttaattctt
    10501 ctgaaagtaa tattagacca attattccgt tggatagatc ttgtttaaaa aaatctatat
    10561 tgttatttga gctttgtttt gctaaataat caatgttttc tcttataaag tctatattta
    10621 gtattttttc tgaatgtttt tttataaaat caattgtttc atcattcata tctttcagcc
    10681 tcctctaatt tttggtattc ggtacaatta tttaacaagt attcatgact accttcagcg
    10741 acaatttcac ccttggacat tacaatgatt ctatccgcat ttttaactgc tcctattcta
    10801 tgtgatatga ataatcccat tttattatga gatataaatt ttttgaattc ataaaataaa
    10861 ttactttcag agatggggtc taatgcagag ttaggttcat ctaatatcac aaaactagca
    10921 tttctattaa aaaaggccct gcttgtagca atgctttgcc attggccacc ggacagttgt
    10981 ttaccgtttc caaaccaatt tccaagagaa tcttcaactt tcaatttttt ataaagatta
    11041 tcattatacg tctttagagt attcataatc ttatcttctt gtttaaggtt acgtatatcg
    11101 ccaaaaccaa tattttcacg tactgacaac tcatactgaa caaaattttg aaataaaacg
    11161 gatattcctt tatggaaaga gtgcttatta agcttattgg aatcgatatt gtttattaag
    11221 tattgccccc ttgttggttt ataaagtcca gtaagaattt taacaatcgt acttttacca
    11281 gatccgttgg cacctacaat tgcaacgatt tctccttttt tgattttaaa gcttatatta
    11341 tctaaagctt tcacactatt tttatatgag taagagacgt ttttaaattc aatcgttgtt
    11401 attggtaggt tataataact tccattatca tctttttcat tatcatctat aaaatcataa
    11461 agtttacgaa gaatttgcaa atcatatttt aaattaaata aattgttaat aaatgtctgt
    11521 gtgcttcctt gagtaagact tattatttga gtcacactgg ttaaggtccc caatagagcc
    11581 ttgcctagat aaattgacca aatcactgat agaacaagta tagctgttac aatactaact
    11641 attaagtcat atacataatt aaatatagag agattcttca aaatttttaa gtcctgtgat
    11701 ataaattctt tttttagatt ggcaaacttt ttctttaaat gttttgataa gtcaaagaaa
    11761 attatttctt tgacgctaaa atcatgagtt aatagatatt ttatatacca agccttacgt
    11821 tctttttcgg ctctattcca atgaacatta aactgattgt tgcttaatat tagtaggaat
    11881 ggaatagaaa tgaaagttaa aagtaccagt gttatgccat atattgggtt tattttgaaa
    11941 atataaaaat aactactaat tatcgtaatg atcgatacac taattgaatt gaatgcggta
    12001 aatacagaaa aaggttttga cggggcttca ttagacagtt tatctataac tgaatatgtt
    12061 tcttcgtttt caaaagctgt tatatccatt ctagatacta catttaaaat gtcatattct
    12121 acttcaaaag acaatctatt acttataatt ccgtttatgt aagactgtac tgatgatata
    12181 gaatctgtta tcaacgaaac tgagatgtat cctgcaaaac acattaaaat caacgaaaaa
    12241 tgagaactta atgataaaaa attgatgatt agttgtacaa aaaatataga aattaaagga
    12301 agtattccta atacaatatt tataaaaatc aggatagaaa ataaaatttt atgttgcttc
    12361 catataagtt gtatcgtttg tttaatctct ttcattgctt gtgattctcc taataactca
    12421 atattctttt ttagtaagat tatcagaaaa tatagtgtat ttccaataaa tttattatga
    12481 tataattatg agaaaaatgg aggtgctctt atggcaaata ttttagaaac taaaaacgtt
    12541 acaaagagtt ttagaaatca gttggctttg gataacgttt ctgttcatat accaaaagga
    12601 gaagtttatt gtttgttagg tcctaatgga gcaggtaaga caactttaat gaagataata
    12661 acaggaatga ttcataaaac atcgggaaaa gtgatctttg atggcaaaga atgggaaaga
    12721 gatgcattaa acgatatagg ttctttaatt gagaatgctc ctatatatgg aaatttaaca
    12781 gccttagaaa atttaaaggt ggttgctact ttaagaaaag ttccattaaa tcatcttgaa
    12841 tctattttag aacaggtagg attggagaat aacaaaaaaa ggtctaaaga tttttcttta
    12901 ggaatgaaac aaagattagg tattggaatg gctatggttt caaatccaaa acttttaata
    12961 ttagatgagc caacgaatgg tttagatcca attggaatac aggaactgag aactctcata
    13021 aaaggactgg caaaaaaaga tattactatt ctaatttcta gtcatgattt gtcggaggta
    13081 gaacacttag cagataatat tgggattata tctggtggaa aactgttatt agaagagaag
    13141 tataataatg aaaaccttga aaaactcttt aatgaaatag tagggggagt atagttatga
    13201 ctagtttaat taatagtgaa tttttaaaaa ttcgtagaac atcaatccct tggctaacta
    13261 ttctagtgcc ttttttctgt gtgtttttag tagctgttgt ttttgcggga aatgagtatt
    13321 ctatgcaaag cattattaat caatggagct taatttgggt actattattc tctgtattgt
    13381 tatctggaat gaccactcat cacgaatcaa tatctactca atataagtca atattaagtt
    13441 caccagttag cttatttaaa tttgaacttt caagattaat catgttagtc gaattgttct
    13501 ttataggaaa tgtattttta gccatactag tatgtatttc taaattttat tttagtagca
    13561 tggtcggaat gggctatata ctactatcaa taattatggt ctctatttca ggattatgga
    13621 cagtgccttt tttcttgcta ataagcagat tatcaaatat gtttgttggc atattaattg
    13681 gtttatttgg tttcttgata gggggagttt tttctggaac tcgttttgga tatttaatcc
    13741 catttacgtg gtcaccagag acagcatctc ctattataag aatgcatgtt aacggggttc
    13801 cgtacagttt aggtactcat tcttttatga atacaacgta cattatctta tcgttatcat
    13861 tattcattgt attggcatta actgaatgtc tactatttac tcgtcaggag gttaaaaaat
    13921 gattgctaga gtttttaagt cagaagctat taagcttatt aatagccata tcttatattt
    13981 ccacatagct gccatggtta tctttccaac tttattagcg gttttttttg gtaagaatca
    14041 ttacccgcta acaaattttg tgttgattcc caactttttt ttaatcattt cctttgtggg
    14101 tcctgtagtg atctcaatag ttgcatctat ggttaacgac agagaaacaa aagccggcaa
    14161 ttataagaat ttattagaca cttcaatcag cccattaaaa gtttttcaat cgcaaatagc
    14221 tttttattgg tcactatatc tgacagaaat catattatca ttttatatat acggtttaat
    14281 tttgaacatt gtatttcatg cacccctatt tccttatttt acgttattag ttttttgtat
    14341 tctattctct ttattagcag taattcagta cgaattgtgt tttttagcat catatatatt
    14401 tggtaccgct ggatctttga ttttaggatt tgcaggattg atattatctg cactaagtga
    14461 aacggtttta ttcgataatg tttgggaatt tattccatgg tcatggcaaa ttagaagtat
    14521 tgcttttttt cagaatggta ctactattaa tgattctatt cattatgcgt tcttatatat
    14581 ctttagtata ttaataagtc tcgtgtcgct gttcgtatgt aataagatct tttataggat
    14641 tttaaataaa aattgaggta ataattatga gaacatatta caacgttgaa aatcctgtcg
    14701 tatttagaaa acacttttgc aatgttgaag gtggagaaaa atttttatat gatgaaagtt
    14761 tgttagatta tttaagcaag tttgaatcgt ttaaagaaca aattagagtg tcaaaccatg
    14821 aattatataa aaattacatg gattacttaa aagatagatc aagtttttca aaaaaaagaa
    14881 ctagacacct tgttgaatca ataacaggat actttctgag aataaagtat agaaatactc
    14941 cttttggtct ctttagtggt gttggagttg taaacaatga aaaacatact aaatacactc
    15001 atctaaaaaa aatagttctt ggatataggt ggaaatatag tgtcataaac atgttagaaa
    15061 aaaggtatcg gtcaaatgtt aaatatgtat ttgaaaataa tatgacagat ataaatggta
    15121 atccgttttt tcttgatgtt ggtaaagaaa gtaacattat tgatggtaaa tattacaatg
    15181 acgaaatagg ctttaaaatt tctaaaaaca aattaacgga actccttgaa aaatttttga
    15241 gtaaaccaag atgcacagat gacattttta atgttattaa aggagaatat aaaaatattg
    15301 atcctagtta tataaaagaa tatatagtca atctggttga aaaaaagatt attaaatcta
    15361 ctttacgcag ttctttatct caaacggacg aagagtggat agaaaacgtc aaagattttt
    15421 taaaagtagt aaaagataat cagtctatac aaaaatttga aacattaaaa tcattaataa
    15481 gctcttattc aataacaaac gtcggtgaag gaatatatct ttttgactct atttccaaat
    15541 gcatgaacga catattccca acgcagaaag atgatctaaa tgtagattta tatttaaatg
    15601 atccgtcttt atcggttaca caggataatc tgaatgattt aaataaatgt atagatatgc
    15661 taactaaaat atctatacct tatgatgagt tacatgatta tacttcatta tttcttgata
    15721 aatttggtat ggatgtagag gttccattat acgaacttat agacaaaaac tcaggaatag
    15781 gttttccaaa atatgattcc tccgataatt actctacgtc taaggaaacg ttggaatcaa
    15841 ttggttcgtt gttagatgaa aaattgtaca ccgctctaat aaacaaaaga ccattaacca
    15901 ttgatcaatc aacagttaat gatgtacaaa gaattttaaa cggttctgat agccacaagg
    15961 atatcattcc aaaatctatg gatgtatgtc taacgcaaga aagtcatgat aataaaatat
    16021 catttacaat ttcagaaata tttggttctc cattaggaag ttcaattaca ggtagattta
    16081 gactattaga tgacttatat caaaaaaaca aagatgtatc agatgattat ataacctgtg
    16141 acataaacgc atttccaaat ttattaagtt tatcaacttt aacgcaaaat cgacatctga
    16201 atgaatacga attgtcggtt ggtactaatg gaaatcaaca caagaatcaa atatcattaa
    16261 atgatttagt tgtagggtat gaaaacgaca ccttatatat aaggtgttcc caaaataata
    16321 aaattgttag atttgttgat tataacatgc taaatggaga cttaaagaac cctgttataa
    16381 agctattact tgcaataagc aatcagtccg atagatggcc atttaatttc ccatggagtt
    16441 ttggtcagcc aacgagaacg gttattcctg aaataatttg taataatata atcgtaagtc
    16501 cattaacatg gaatattaat actgctaact atgacaatag ctttgaagat ttttatcgtg
    16561 aattcatttg taaaatagac tctcttcaag ttccaaaatt attttacttt gtagaatcag
    16621 ataataaaat tgtaagtgat agaaaaaaag agtctattta caatatttat aaacatttaa
    16681 aaaaatctaa ttctaaaaat gcaaaattac aaaaatgtga atttggcagc ttaaatgatg
    16741 acggaaaaac tcaagaaata gtagttactt taaaaaacaa atataaaaac aataacccca
    16801 ttaaccagaa gccaatatat agatcttgtg actatttaaa acctttgaat gtagttaatg
    16861 gatggtttta tgtagaggtt gagtttggtg acgttgggat aaaaaatgaa tttataatac
    16921 aagagttatc aaaattaaat gatgatttaa ataatttgta tgatttattc ttttttatac
    16981 aatacgaaaa caaagacaat ccaactattc gtttgagatg gaaaagtaat gatatcttta
    17041 ttgtgcagaa tcgtgtaacg aagtatctag actcgttgat taaagaaggt attattaaaa
    17101 attatactaa taataactac tttagagagt tagatagata tggtggaaaa caaaatattg
    17161 acaaagctga gcaagctttt cattatgata cacttttttc aattgtttct ttccaaaaca
    17221 atccggaaaa gattgaaaaa gaatcactag ccatatactc ttgcgtatcg atgctcctat
    17281 ccatttctaa ttccatagat acaataattg atttcttaaa catatatgat tatccatata
    17341 atattaataa aaatgcatct ggttggataa ggaaaaacga aaaatatatg gatgaatatt
    17401 taaacaattt aacaatcgaa accaaatcat tgttagatag acgtaataat gaaataaacg
    17461 acattattag caatagcgat cgaaatcaag taaacattgt aagtagcttt ttgcatatgt
    17521 ctataaatag aattttaggc actgatagat atggtgaaca acaaataatg ggtatattac
    17581 taaagctatt taataaagcc aaatacacaa atgaaattac gggttgcaaa ttatatacaa
    17641 acaatagcga aagttaatta acttaacccg atttgctaac tattaatgga taatgagtgc
    17701 attgctatct acattattac tatgattcat ctattcaatg aatcataaaa gaggttacaa
    17761 gttttttgtc atccgagtga agatgcttat attcataaat aggtggggaa agtggtcatc
    17821 attattgtgt aataaaaaat ttaaataata ctactttcaa tagaataaaa ttattatcac
    17881 ataaatgtat ggtatgatga tgattattat taataaatta cttataatta ataataattt
    17941 tttaggagtg gttgtaatcg gaaaattaaa aaaatatttc aaagcattct atcaagatga
    18001 tttcgagaat gaattcttac aagatgatag aacagttata gcaatagata caaattactt
    18061 gttatctata ttgagattgg acccagaggt tgcaaataaa tatattgatg cgttggaaaa
    18121 aaataaggaa agaatatata taccatactt tgttggttta gaattttctt ttaataaaat
    18181 caacgtgaaa attgatcaag ataacaaatt aaaggatata gataataaaa taaacaagtt
    18241 aaaaaaccaa ttagattcca cgtcacaaga aatttcaaaa ttaactaata gcaataaatt
    18301 agataatatg aaaaagattt ctaatgagta tattgaacaa tttaaagggc ttatagatga
    18361 tagttctacg atagaagaaa atcgtcaaga aaattattta aagttgatag atattattga
    18421 cgatttattg ggagaaaaac ctgaccagac ttttattgac aacattgaga aagaaggtaa
    18481 gttgagatac gaagaagaac ttcccccagg atttgatgat tctaaaaaag atggaactag
    18541 acattttggt tctttaacat atcatcaaaa atatggagat ctcttgatat ggagagaaat
    18601 tctaaagcat ttttcttcta gcaagaatag taaacgacta atatatataa ctgatgatgg
    18661 tatatctaat aagaagaacg atttatttta taagtatggg ggcagaactg tcggtccaca
    18721 tatacactta atagatgaag ctaatgaagt ggctaatcta gatttatata taattgatag
    18781 taaatccttt gttaaatatt ctaacgtttc tttaaataaa tctgatgaaa gtcaaataaa
    18841 taatttattg aataccagat acgtgagtaa tgccggatac atgagtgttg atgtaaggga
    18901 ctttgaagat tctacagatg ataatattct agaagattct caaagttatc atatccgcaa
    18961 caaaatagat ttgtgcttgg atcaacacta tttaaatagc ttatttacta atgatttatt
    19021 tggtaatagg agtagtattg atgaggtttg tacaacaatt ttaaatattt ctaaaaaatt
    19081 atgcatgtta tataaacaat gtaatgattt agacagagag tatcttattt ctcagtatat
    19141 tagtttcatg ctaaacattt attccagtat tgatgataaa gaagataaag tcttatttat
    19201 taaaagtcta aaaaaatgtt taacaccttt atatttcgtt agtgatttaa aacacaagga
    19261 taagctatca tcactaacag actatcttgt ggaaaaatca tataaatatt acaaacttaa
    19321 gtctcatgac ttaaaagaat agacaaccac ccattaacat aaattcgaaa tttaactgtt
    19381 tttatgcaaa aatattaata ctttattgca tatgaggaga atgttaatac aaagtcactt
    19441 atactatgca aaaaatatat gcaaaaaata tacaaaaaaa tccatctctt attaagag
//
