LOCUS       NZ_LIYH01000010        18658 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB03234 scaffold10, whole genome shotgun sequence.
ACCESSION   NZ_LIYH01000010
VERSION     NZ_LIYH01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB03234
  ORGANISM  Streptomyces sp. CB03234
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 212667)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212667)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905885.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:52:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,127
            CDSs (total)                      :: 7,053
            Genes (coding)                    :: 6,894
            CDSs (with protein)               :: 6,894
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 70 of 159
            Pseudo Genes (incomplete)         :: 112 of 159
            Pseudo Genes (internal stop)      :: 10 of 159
            Pseudo Genes (multiple problems)  :: 33 of 159
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 194009
            Orig. end                         :: 212667
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIYH01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..18658
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..18658
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..18658
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:16587](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..16587
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(857..1798)
                     /locus_tag="AMK26_RS34960"
                     /old_locus_tag="AMK26_34380"
     CDS             complement(857..1798)
                     /GO_function="GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049646.1"
                     /locus_tag="AMK26_RS34960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34380"
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_073760228.1"
                     /transl_table=11
                     /translation="MGAGHDHGHTHGGPPPTGTAAAAYKGRLRIALTITLAVMVAEIVG
                     GVIADSLALIADAAHMATDAVGLAMALLAIHFAGRPADQRRTFGFARAEILAALANCLL
                     LLGVGGYLLFEAVQRFITPAETKGGVAIAFALFGLVANMVSLSLLVRGQKESLNVRGAY
                     LEVLADALGSVTVLVSAGIILATGWQAADPIASLVIGLMIVPRTLKLLRETLDVLLEAA
                     PKGVDMAEVRAHILELPGVLDVHDLHAWTITSGMPVLSAHVVVHQEALDSVGHEKLLHE
                     LQGCLGDHFDVEHCTFQLEPSGHAAHEAKLCH"
     misc_feature    complement(1812..1825)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.86"
                     /tool="antismash"
     misc_feature    1868..1882
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.72"
                     /tool="antismash"
     misc_feature    complement(2058..2072)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: strong, score: 20.76"
                     /tool="antismash"
     gene            2222..4291
                     /locus_tag="AMK26_RS34965"
                     /old_locus_tag="AMK26_34385"
     CDS             2222..4291
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018492025.1"
                     /locus_tag="AMK26_RS34965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34385"
                     /product="DUF5941 domain-containing protein"
                     /protein_id="WP_107421115.1"
                     /transl_table=11
                     /translation="MSTAILTGPPVPGSPLEGDLRSLGFDVRYAADERETAALLAAVPA
                     AERVAVVDPRFVGHVHALRLALTDPRFPAATAPGALTAGPEARPALTGALTSVTSATAE
                     PAPASGTAAPPARPDALTSVTPANDATGPAATASAPGTTRNATDPAARRAPDPSGTAPA
                     ADAGTVTAGVPQAQAPRTAAGAAAEQAGRPDRGVVTLADRLAEALEADGVQVHRPELGS
                     LVAAVPVDPQSRKETRDAVAAVDDEAVRLRSAVKARDGFFTTFFISPYSRYIARWCARR
                     GLTPNQVTTASLLTALVAAGCAATGTRGGYVAAGLLLLFSFVLDCTDGQLARYSLQYST
                     MGAWLDATFDRAKEYAYYAGLALGAARGGDDVWALALGAMVLQTCRHVVDFSFNEANHD
                     ATANTSPTAALSDKLDAKGWTVWVRRMIVLPIGERWAMIAVLTAVATPRVVFWALLVGC
                     AFAACYTTAGRLLRSLTRKARRTDRAAQALADLADSGPLAELVAMAVRRRAFPPARTRV
                     VIAFAGAAWLLLGVCLAPMGSAIVIGAVLYVLTSGTAVAHSLKGALDWLVPPVFRAAEY
                     VTILVLAARSEAPGALPAAFGLVAAVAYHHYDTVYRIRGGTGAPPAWLVRTIGGHEGRT
                     LVVAVLATALAAGSHFTLALTALAVAVALVVLVESIRFWVSSGAPAVHDEGEPA"
     gene            4288..5025
                     /locus_tag="AMK26_RS34970"
                     /old_locus_tag="AMK26_34390"
     CDS             4288..5025
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064: glucose-1-phosphate
                     adenylyl/thymidylyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955403.1"
                     /locus_tag="AMK26_RS34970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34390"
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="WP_073760232.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 3.5e-13, bitscore:
                     43.7, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGLVLAAGAGRRLRPYTDTLPKALVPVDGDTTVLDLTLANFAAV
                     GLTEAAVVVGYRKEAVYARKAELEATYGLKLTLIDNDKAEEWNNAYSLWCAREVLKQGV
                     ILANGDTVHPVSVEKTLLAARGNGQKIILALDTVKNLADEEMKVITADGQGVRRITKLM
                     DPATATGEYIGVTLIEPEAAEDLADALKTTFERDPDLYYEDGYQELVDRGFQIDVTPIG
                     DVKWVEIDNHDDLAKGREIACLY"
     gene            5013..6074
                     /locus_tag="AMK26_RS34975"
                     /old_locus_tag="AMK26_34395"
     CDS             5013..6074
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DHQ_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1181: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452391.1"
                     /locus_tag="AMK26_RS34975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34395"
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="WP_073760234.1"
                     /sec_met_domain="Fe-ADH (E-value: 3.7e-20, bitscore: 65.5,
                     seeds: 218, tool: rule-based-clusters)"
                     /sec_met_domain="DHQ_synthase (E-value: 8.4e-11, bitscore:
                     35.0, seeds: 76, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVLTRLIPSPVVVDISCGAMDGLAGLLVDQRISASGKLAMAISN
                     GSGQALREKLAPLLPDADWYAVEDGSIDSAVRLADAIKGKRYDAVVGLGGGKIIDVAKY
                     AAARVGVPMVSVATNLSHDGICSPVSTLDNDNGRGSYGVPSPIAMVIDLDVIREAPARF
                     VRSGIGDAISNISAIADWELSHRRTGEQVDGLAAAMARTAGESVLRHPGTVADDEFLIV
                     LAESLVLTGIAMSISGDTRPASGACHEISHAFDLLHPARAALHGEQVGLGAAFAMHLRG
                     AHEQSGLFAEVLRRHGLPVLPEEIGFSPDEFVAAVEYAPQTRPGRFTILEHLDLSTDQI
                     RDAYADYAKTIRS"
     gene            6052..6831
                     /locus_tag="AMK26_RS34980"
                     /old_locus_tag="AMK26_34400"
     CDS             6052..6831
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049651.1"
                     /locus_tag="AMK26_RS34980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34400"
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="WP_073760236.1"
                     /transl_table=11
                     /translation="MPRPSVAELRPVVHPAGVKDRRSGEHWGGRLYMREISLRITRLLV
                     TTKVTPNQLTYVMTLAGVLAAPALLIPGIPGALLGALMVQLYLLLDCVDGEVARWKKQF
                     SLGGVYLDRVGAYLCDAAVLVGFGLRAADLWGSGRIDWLWAFLGTLAALGAILIKAETD
                     LVGVARHQGGLPPVKESASEPRSSGMALARKAAAALKFHRLVLGIEASLLIVVLAVLDT
                     VRGDLFFSRLGVAVLAGIALLQTVLHLVSILVSSRLK"
     gene            6840..7712
                     /locus_tag="AMK26_RS34985"
                     /old_locus_tag="AMK26_34405"
     CDS             6840..7712
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888991.1"
                     /locus_tag="AMK26_RS34985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34405"
                     /product="glycosyltransferase"
                     /protein_id="WP_073760238.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.4e-11,
                     bitscore: 37.4, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLGAVIITMGNRPDDLRALIESVAEQDGDPIQVVVVGNGAPVTG
                     VPEGVRTIDLPENLGIPGGRNVGIEAFGPGGTDVDALLFLDDDGLLANTDTAELIRQAF
                     TADPELGIISFRIADPDTGVTQRRHVPRLRASDPMRSSRVTTFLGGANAVRTKVLAEVG
                     GLPDEFFYAHEETDLAWRALDAGWMIDYRADMVLFHPTTAPSRHAVYHRMIARNRVWLA
                     RRNLPAPLVPLYLGVWLLLTLARRPSVPALKAWFGGFKEGWTTPCGPRRPMKWRTVWRL
                     TRLGRPPVV"
     gene            7830..8759
                     /locus_tag="AMK26_RS34990"
                     /old_locus_tag="AMK26_34410"
     CDS             7830..8759
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964854.1"
                     /locus_tag="AMK26_RS34990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34410"
                     /product="ABC transporter permease"
                     /protein_id="WP_073760240.1"
                     /transl_table=11
                     /translation="MSETTHDGAIAVTAPPSPDAGLSPAELAAKYGLSVSGARPGLAEY
                     VRQLWGRRHFILAFSQAKLTAQYSQAKLGQLWQVATPLLNAAVYFLIFGLILNAGRGME
                     KEVYIPFLVTGVFVFMFTQTSVMSGVRAISGNLGLVRALHFPRASLPISFALQQLQQLM
                     FSMIVLFVIAAAFGSYPSLSWLLILPALALQFLFNVGLSLIMARLGSKTPDLAQLMPFV
                     MRTWMYGSGVMFSIPIMLKDKPAWIADVLMYNPAAVYMDLIRFALIDGYGSQNLPSHVW
                     LAALLWAVLVGAAGFVYFWKAEERYGRG"
     gene            8752..9564
                     /locus_tag="AMK26_RS34995"
                     /old_locus_tag="AMK26_34415"
     CDS             8752..9564
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009188923.1"
                     /locus_tag="AMK26_RS34995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34415"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_241838229.1"
                     /transl_table=11
                     /translation="MAEQTDDVTATAAADGARIPTVIADDVHIVYRVNGGGGGKGSATA
                     ALSRMIRRDKGESRGVRKVHAVRGVSFTAYRGEAIGLIGTNGSGKSTLLRAIAGLLPTE
                     KGKVYTDGQPSLLGVNAALMGDLTGERNVILGGLAMGMSREEIRERYQEIVDFSGINEK
                     GDFITLPMRTYSSGMGARLRFAIAAAKNHDVLMIDEALATGDRKFQIRSEQRIRELRKE
                     AGTVFLVSHSNKSIRDTCDRVLWLEKGELLMDGPTDEVLRAYEKETGR"
     misc_feature    9686..9700
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.66"
                     /tool="antismash"
     gene            10001..10906
                     /gene="hpnC"
                     /locus_tag="AMK26_RS35000"
                     /old_locus_tag="AMK26_34420"
     CDS             10001..10906
                     /EC_number="2.5.1.21"
                     /GO_function="GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017236664.1"
                     /locus_tag="AMK26_RS35000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34420"
                     /product="squalene synthase HpnC"
                     /protein_id="WP_073760242.1"
                     /sec_met_domain="phytoene_synt (E-value: 1e-40, bitscore:
                     133.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 3.6e-41,
                     bitscore: 134.8, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGTRQVRPDASPRTTLDKAAHENFPVAPFFLPRAWRDDLMAVYG
                     YARLVDDIGDGDLAPGGADARHLGAEGETDRLALLDAFEADLRRVFDGTPSHPLLRALK
                     TTVRRHALTPEPFLGLIEANRQDQVVRRYQTYDELVAYCELSANPVGRLVLDITGTTSP
                     ERLRRSDAVCTALQIVEHLQDVAEDLAHDRIYLPAEDMKRFHVTEADLARPTGGASVRA
                     LIAYEAERARRLLDDGIPLVGSVRGRLRLLLAGFVAGGHAALDAITAAGHDVLPGPPKA
                     TGPKLMREVGTVLRRARREG"
     gene            10903..11853
                     /gene="hpnD"
                     /locus_tag="AMK26_RS35005"
                     /old_locus_tag="AMK26_34425"
     CDS             10903..11853
                     /EC_number="2.5.1.103"
                     /GO_function="GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072792.1"
                     /locus_tag="AMK26_RS35005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34425"
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="WP_198944247.1"
                     /sec_met_domain="phytoene_synt (E-value: 1.6e-58, bitscore:
                     191.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 3.2e-70,
                     bitscore: 230.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGTVEGQTEMSAPVAAAYSYCEAVTGRQARNFAYGIRLLPTGKR
                     QAMSALYAFSRRVDDIGDGTLAPEAKQERLETTRELLRRIREGAVGQDDTDPVAVALAD
                     AARRFPLPLGGLDELIDGVLMDVRGETYETWDDLKVYCRCVAGAIGRLSLSIFGTTPGA
                     RGAARAPEYADTLGLALQLTNILRDVREDAGTGRTYLPADDLAKFGCSDGFRTATPPPG
                     SDFTGLVHYEVRRARALFAEGYRLLPMLDRRSGACVAAMAGIYRRLLDRIERDPEAVLR
                     GRVSLPGHEKAYVAVRGLSGLDARTVSRQTVRRRA"
     gene            11958..13358
                     /gene="hpnE"
                     /locus_tag="AMK26_RS35010"
                     /old_locus_tag="AMK26_34430"
     CDS             11958..13358
                     /EC_number="1.17.8.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890661.1"
                     /locus_tag="AMK26_RS35010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34430"
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="WP_073760246.1"
                     /sec_met_domain="DAO (E-value: 2.8e-08, bitscore: 27.2,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDADLTPGHAAPGHAVVVGGGLAGITAALRLADSGLRVTLLEGR
                     PRLGGLAFSFKRGDLTVDNGQHVYLRCCTAYRWFLDRVDGAHLAPLQPRLDVPVLDVAH
                     PRGPRLGRLRRTALPVPLHLAASLATYPHLSLAERASVGRAALALKRLDPADPALDGID
                     FATWLARQGQSARATEALWDLIGVATLNATAPHSSLALAAMVFKTALLTDPGAADIGWA
                     HVPLGDLHDTLARKALDAAGVRTELRAKVSAILPSAHGRWAVDVPGERLEADTVVLAVP
                     QREAHDLLPHGALDDPGTLLDIGTAPILNVHVLYDRKVLRRPFFAALGSPVQWVFDRTD
                     ASGYTDGQYLALSQSAAGDEIDEPVSVLRERYLPELERLLPAARGAKVRDFFVTRERTA
                     TFAPTPGVGRLRPGARTNAPGVHLAGAWTATGWPATMEGAVRSGFTAAGAALSALGRPH
                     VHPLEEAA"
     gene            13355..14455
                     /locus_tag="AMK26_RS35015"
                     /old_locus_tag="AMK26_34435"
     CDS             13355..14455
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964849.1"
                     /locus_tag="AMK26_RS35015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34435"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_179166849.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.3e-60, bitscore:
                     198.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLSTGTRGETVPTVPPVNPAVDTADVTALLERGRTMSTPVLRTA
                     VKRLAPPMDTVAAYHFGWIDAHGRPADGDSGKAVRPALALLSAEAAGVAPEVGIPGAVA
                     VELVHNFSLLHDDLMDGDEQRRHRDTVWKVHGPAQAILVGDALFALANELLLELGTVEA
                     GRATRRLTTATRKLIDGQAQDISYEHRERVTVEECLEMEGNKTGALLACACSIGAVLGG
                     ADDRTADTLEAYGYHLGLAFQAVDDLLGIWGDPEATGKQTWSDLRQRKKSLPVVAALAA
                     GGPASERLGELLAADAKSSDLDSFSEEEFAMRAALIEEAGGRAWTSEEARRQHAVAIEA
                     LHRVDMPERVRAQLTALADFVVVRKR"
     gene            14575..16587
                     /gene="shc"
                     /locus_tag="AMK26_RS35020"
                     /old_locus_tag="AMK26_34440"
     CDS             14575..16587
                     /EC_number="5.4.99.17"
                     /GO_function="GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="shc"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016639269.1"
                     /locus_tag="AMK26_RS35020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34440"
                     /product="squalene--hopene cyclase"
                     /protein_id="WP_073760250.1"
                     /sec_met_domain="T2TS (E-value: 1.8e-152, bitscore: 502.1,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATTDGSTGALEPRAASATGTTTGTTTTTTTPAANDVREVAARA
                     AQRSIEYLLARQDDEGWWKGDLETNVTMDAEDLLLRQFLGIQDEATTRAAALHIRGEQR
                     DDGTWATFYGGPGELSTTIEAYVALRLAGDRPDEPHMARAAAWIREQGGIAAARVFTRI
                     WLALFGWWKWEDLPELPPELVFLPKWFPLNIYDFGCWARQTIVPLTVVSAKRPVRPASF
                     PLDELHTDPRLPNPPRTLAPAGTWEGAFQRLDKALHAYHQVAPRWLRKAAMNTAARWII
                     ERQENDGCWGGIQPPAVYSLIALHLLGYDLEHPVMRAGLESLDRFAVWRDDGARMIEAC
                     QSPVWDTCLATIALADAGVRPDHPALVKAADWMLGEEIVRPGDWAVQRPGLTPGGWAFE
                     FHNDNYPDIDDTAEVILALRRVKHPHPEQIEAAIRRGANWTAGMQCRDGAWAAFDADNT
                     SPFPNLLPFCDFGEVIDPPSADVTAHVVEMLAHEGKAHHPRARRGITWLLAEQEPSGAW
                     FGRWGVNYVYGTGSVVPALTAAGLPPSHPAIRRAVAWLESVQNDDGGWGEDLRSYRERE
                     WIGHGASTASQTAWALLALLAGGQRESRAVERGVRWLAGTQREDGSWDEPYFTGTGFPW
                     DFSINYHLYRQVFPLTALGRYVNGEPVVGRLGKGA"
     gene            16587..17279
                     /locus_tag="AMK26_RS35025"
                     /old_locus_tag="AMK26_34445"
     CDS             16587..17279
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890664.1"
                     /locus_tag="AMK26_RS35025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34445"
                     /product="1-hydroxy-2-methyl-2-butenyl 4-diphosphate
                     reductase"
                     /protein_id="WP_073760252.1"
                     /transl_table=11
                     /translation="MAAEPEPSGSAHASAAAPVPPMLIACALGIEQLALRSGRRRHGGT
                     SPGPVTVLRTGMGPANAQRAVTRALEGGELRGAAVVASGFCAGLEPGMHPGDLVVADET
                     RGPGGSTLCTGTDLLVDALTRAVPGRTVHSGPLTGSDHVVRGQERAQLRGTGAIAVDME
                     SAATLRSALNTGVRSRPVAAVRVVVDAPEHELVRIGTVRGGISAFRVLRSVLPAFYEWH
                     RSLLLPRR"
     gene            17285..18307
                     /gene="hpnH"
                     /locus_tag="AMK26_RS35030"
                     /old_locus_tag="AMK26_34450"
     CDS             17285..18307
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890665.1"
                     /locus_tag="AMK26_RS35030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34450"
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="WP_198944248.1"
                     /sec_met_domain="PF04055 (E-value: 4.1e-19, bitscore: 63.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 6e-10, bitscore: 32.4,
                     seeds: 32, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAMPLRQTIRVGTYLFEQKLRKREKFPLIVELEPLYACNLKCEGC
                     GKIQHPAGVLKQRMPVAQAVGAVLESGAPMVSIAGGEPLMHPQIHEIVRQLVAKRKYVF
                     LCTNALLLRKKIEDFTPSPYFAFAVHIDGLRERHDESVAKEGVFDEAVEAIKEAKRRGF
                     RVTTNSTFFNTDTAQTVIEVLNFLNDDLKVDEMMISPAYAYEKAPDQEHFLGVEQTREL
                     FRKAFSGGNRRRWRLNHSPLFLDFLEGKADFPCTAWAIPNYSLFGWQRPCYLMADGYVP
                     TYRQLIEETDWDKYGRGKDPRCANCMAHCGYEPTAVLATMGSLKESLRAARETVSGSRG
                     "
     gene            18312..>18658
                     /locus_tag="AMK26_RS35035"
                     /old_locus_tag="AMK26_34455"
     CDS             18312..>18658
                     /EC_number="1.17.7.3"
                     /GO_function="GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA]"
                     /GO_process="GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018534474.1"
                     /locus_tag="AMK26_RS35035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_34455"
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="WP_158071173.1"
                     /transl_table=11
                     /translation="MPEPVGLGLPELPPRPIAVRRPSRRIQVGSVAVGGDAPVSVQSMT
                     TTRTSDVGATLQQIAELTASGCQIVRVACPTQDDADALAVIAKKSQIPVIADIHFQPKY
                     VFAAIDAGCAAV"
ORIGIN
        1 cggccatgcc gggcgccgcc gggtcggccg cgtcggcccg ctcgccgagc ggcccgtagt
       61 actcgtccca gtcgctctcg ggctggggat acgtcccgag cacgtggtac ccggccgcga
      121 cggccgccgc ggtgttctcg gcggtggtac gcagcgcgta gtgcctgtcc cagaaggccc
      181 gtgcctcggc gctcggctcg tcggccgtcc acccgcactc ggtgacgacc agcgtcccgc
      241 cgggggagag cagccggcgc caggaacgca gcgcgcggtc gaagccgatc gagtacgccg
      301 agctctccgc ccagaccagg tcgaacgacc cgtcggcgta cggcagcgcg gccatgtcgg
      361 cccggacggt acggacggag cccgcgagcc cccgggcatc ggcggcccgc cgcagctcgg
      421 ccaggaacgg ctcgtgcagg tcgacggccg tgacgtccgc accgccgccg gtgccggcct
      481 cttcggccag cagcagcgcg gaccggccgg ggccgcagcc caggtcgagc acgcgcgggt
      541 gcggcggcag cggaccggcg agggagagga gccggcgcgt ggtgtcaccg gaaccggggc
      601 cctgccgcgg aagaccgtgg tgcagggcga agaaagcgtc gtactcagcg ttgtcggaca
      661 acgtgggaaa ccctttgatg tgagcgggcc ccggccgacg tgtacgtgac gagacgtacg
      721 ggatcaggtc agccgagaac ccggaagccg gaggcgaagc tggaccgggc gctgcactgg
      781 gcagccgtca ccgcggcggt catcaacctc agctcctctc acaggtacga ctttcgatca
      841 cccaccctaa cagcggtcag tgacagagct tcgcctcgtg cgcggcatgg ccgctcggct
      901 ccagctggaa cgtgcagtgc tcgacgtcga agtggtcgcc caggcacccc tggagctcgt
      961 gcagcagctt ctcgtgcccc accgagtcga gcgcctcctg gtgcaccacc acatgcgccg
     1021 acagcaccgg catcccggac gtgatggtcc acgcgtgcag gtcatggacg tccagcacac
     1081 ccggcagctc gagtatgtgc gcccgtacct ccgccatgtc cacgcccttg ggggccgcct
     1141 ccagcagcac gtcgagcgtc tcgcgcagca gcttcagcgt gcgcgggacg atcatcaggc
     1201 cgatcaccag ggacgcgatc gggtcggccg cctgccagcc ggtcgccagg atgatcccgg
     1261 ccgagaccag caccgtcacc gagcccagcg cgtccgcgag cacctccagg tacgcgccgc
     1321 gcacgttcag gctctccttc tgcccccgta cgaggaggga cagcgacacc atgttggcga
     1381 ccaggccgaa cagggcgaac gcgatcgcca ccccgccctt cgtctcggcc ggcgtgatga
     1441 accgctgcac cgcctcgaac agcaggtagc cgcccacgcc gagcagcagc agacagttcg
     1501 ccagcgcggc caggatctcc gcccgtgcga acccgaacgt acgcctctgg tcggccgggc
     1561 ggccggcgaa gtggatggcc agcagcgcca tggcgagacc gacggcatcg gtcgccatgt
     1621 gcgcggcgtc cgcgatcagc gcgagcgagt cggcgatgac gccgccgacg atctcggcca
     1681 ccatgaccgc gagggtgatc gtgagcgcga tccgcagccg tcccttgtac gcggcggccg
     1741 cggtccccgt gggcggcggc ccgccgtgcg tgtgcccgtg atcgtgccct gcccccatgg
     1801 acggcctccc gttgatcgac gacaacggcc agtgaactac gggtgggggg tatccgcaag
     1861 ccggtactga acaccgttgt catgtgccct gacctgcggt gatgtcctcc tggtcagggc
     1921 ggcgcgatcg ccctggccgc gccgcccggc cgccgccccc gcctgcccgc tcccgccagg
     1981 gccgggccac ctgggcgccg cgtcctggcg gccggcccgt gcggtcgccc acccgcggtt
     2041 tgtggggtga ggtgcccatc gacgatgatg atcgcgtcct ggcggaaggc cgccggatgc
     2101 cgggaaacct caatgaactt ccgcttccgt tcatccgata gcctctgccg acgtgccgcc
     2161 gcccgtgacc gggccgagcc gccgtgcacc agccctgttc agctccaagg agtgagttcg
     2221 tctgtcgacc gccatcctca ccggtccgcc ggtacccgga tcgccgctcg agggcgatct
     2281 gcggtcgctg ggcttcgacg tccggtacgc cgccgacgag cgggagaccg ccgcactgct
     2341 cgccgccgtc ccggccgccg agcgcgtcgc ggtcgtcgac ccgcgcttcg tgggccacgt
     2401 ccacgcactg cgcctcgccc tgacagaccc ccgcttcccg gccgccacgg cccccggcgc
     2461 cctcacggcc gggccggagg cccgccccgc cctgaccggc gcgctgacgt ccgtgacgtc
     2521 ggccaccgcc gagcccgcgc ccgcctcggg taccgccgcc ccgccggcgc gccccgacgc
     2581 gctgacgtcc gtgaccccgg cgaacgacgc caccgggccg gccgccaccg cctcggcacc
     2641 cggtaccacc cggaacgcca cggaccccgc tgcccggcgt gcccccgacc cgtccggcac
     2701 cgccccggcg gccgacgccg gcaccgtcac ggccggcgtg ccgcaggcgc aggccccccg
     2761 gaccgccgcc ggtgccgccg ccgagcaggc cggacgcccc gaccggggcg tcgtcaccct
     2821 ggccgaccgg ctcgccgagg ccctggaggc cgacggggtc caggtgcacc ggcccgagct
     2881 cgggtcgctc gtcgccgccg tacccgtcga tccgcagagc cgcaaggaga cccgggacgc
     2941 cgtcgcggcc gtcgacgacg aggccgtacg cctccgctcc gccgtgaagg cgcgtgacgg
     3001 tttcttcacc accttcttca tcagccccta ctcgcgctac atcgcccgct ggtgcgcccg
     3061 ccggggcctc acccccaacc aggtcaccac cgcctccctc ctcaccgcgc tcgtcgcggc
     3121 cggatgcgcg gcgaccggca cccgcggcgg gtacgtcgcc gccgggctgc tgctcctctt
     3181 ctccttcgtc ctggactgca ccgacgggca gctcgcccgc tactcgctgc agtactcgac
     3241 gatgggcgcc tggctggacg cgaccttcga ccgcgccaag gagtacgcct actacgcggg
     3301 cctcgccctc ggcgccgcgc gcggcgggga cgacgtatgg gcactggcgc tcggcgcgat
     3361 ggtcctgcag acctgccgtc acgtcgtgga cttctccttc aacgaggcca accacgacgc
     3421 caccgccaac accagtccca ccgccgccct ctccgacaag ctcgacgcca agggctggac
     3481 ggtctgggtg cgccggatga tagtcctgcc catcggcgaa cgctgggcca tgatcgccgt
     3541 cctcacggcg gtcgccaccc cccgcgtcgt cttctgggcc ctgctcgtcg gctgcgcttt
     3601 cgccgcctgc tacaccaccg ccggccgcct cctgcggtcc ctcacccgca aggcccgccg
     3661 taccgaccgg gccgcccagg ccctcgccga cctcgccgac tccggtccgc tcgccgagct
     3721 ggtcgccatg gccgtccggc gccgcgcctt cccccccgcc cgcaccaggg tcgtcatcgc
     3781 cttcgccgga gccgcctggc tcctcctcgg cgtctgcctc gcgcccatgg gcagcgccat
     3841 cgtcatcggg gcggtcctct acgtcctcac ctccggcacc gccgtcgccc actccctcaa
     3901 gggcgccctc gactggctgg tcccgcccgt cttccgggcc gccgagtacg tcacgatcct
     3961 ggtcctggcc gcccgctccg aggccccggg agcgcttccg gccgcattcg gcctggtggc
     4021 ggccgtcgcc taccatcact acgacacggt ctaccgcatc cgcggcggca ccggcgcgcc
     4081 gcccgcgtgg ctggtgcgga cgatcggcgg acacgagggc cgcacgctgg tggtggcggt
     4141 cctcgccacg gccctggcgg ccgggagcca cttcaccctc gcgctcaccg ccctcgcggt
     4201 ggccgtcgcg ctggtcgtgc tcgtggagtc catccgcttc tgggtgtcct ccggagcacc
     4261 cgcagtacat gacgaaggag aacccgcatg atcggcctcg tactcgcagc cggtgccgga
     4321 cggcgtctgc gcccctacac cgacacgctc ccgaaggccc tcgtgccggt ggacggggac
     4381 accacggtcc tcgacctgac cctcgccaac ttcgccgcgg tcggtctcac cgaggccgcc
     4441 gtcgtcgtcg gctaccgcaa ggaagcggtc tacgcgcgca aggccgagct ggaggccacg
     4501 tacggcctga agctcaccct catcgacaac gacaaggccg aggagtggaa caacgcctac
     4561 tccctgtggt gcgcccgtga ggtcctgaag cagggcgtca tcctcgccaa cggcgacacc
     4621 gtgcacccgg tctccgtcga gaagaccctc ctcgccgccc gcgggaacgg ccagaagatc
     4681 atcctcgccc tcgacacggt gaagaacctc gccgacgagg agatgaaggt catcaccgcc
     4741 gacggccagg gcgtgcgccg catcaccaag ctgatggacc ccgccaccgc caccggcgag
     4801 tacatcggcg tcaccctcat cgagcccgag gccgccgagg acctcgccga cgcgctgaag
     4861 accaccttcg agcgcgaccc cgacctctac tacgaggacg gctaccagga gctggtggac
     4921 cggggcttcc agatcgacgt gacgcccatc ggcgacgtca agtgggtcga gatcgacaac
     4981 cacgacgacc tcgcgaaggg ccgtgagatc gcgtgcctgt actgacccgc ctcatcccct
     5041 cgcccgtcgt cgtcgacatc tcttgcgggg cgatggacgg cctggccggc ctcctggtcg
     5101 accagcggat ctccgcctcc ggcaagctcg ccatggcgat cagcaacggc tccggccagg
     5161 ccctgcgcga gaagctcgcg ccgctgctgc cggacgccga ctggtacgcc gtggaggacg
     5221 gcagcatcga ctccgccgtc cgcctcgccg acgccataaa gggcaagcgg tacgacgccg
     5281 tggtcggcct cggcggcggc aagatcatcg acgtggcgaa gtacgccgcg gcgcgggtcg
     5341 gcgtccccat ggtctccgtg gcgacgaacc tctcgcacga cggcatctgc tcgccggtct
     5401 ccacccttga caacgacaac ggccgcggtt cctacggcgt cccctcgccg atcgccatgg
     5461 tcatcgacct cgatgtgatc cgggaagccc cggcgcggtt cgtccgttcc ggcatcggcg
     5521 acgcgatctc caacatctcg gcgatcgcgg actgggagct gtcccaccgg cgcaccggcg
     5581 agcaggtcga cggcctggct gccgccatgg cccgtacggc gggcgagtcc gtgctgcgcc
     5641 accccgggac cgtcgccgac gacgagttcc tgatcgtgct ggcggagtcc ctcgtcctga
     5701 ccggcatcgc catgtcgatc agcggcgata cccggcccgc ctcgggcgcc tgccacgaga
     5761 tctcgcacgc cttcgacctg ctgcacccgg cgcgcgccgc gctccacggc gagcaggtcg
     5821 gactcggtgc cgccttcgcc atgcacctgc gcggcgccca cgagcagtcg gggctcttcg
     5881 ccgaggtgct gcgcaggcac ggcctgccgg tgctgcccga agagatcggg ttctccccgg
     5941 acgagttcgt cgccgccgtg gagtacgcac cgcagacccg ccccggccgc ttcaccatcc
     6001 tcgagcacct cgacctgtcc accgaccaga tcagggacgc gtacgccgac tatgccaaga
     6061 ccatccgtag ctgaactccg cccggtcgtc catccggccg gagtgaagga ccgtcgcagc
     6121 ggtgagcact ggggcggccg cctgtacatg cgcgagatct cgctgcgcat cacccgcctg
     6181 ctggtcacca ccaaggtcac cccgaaccag ctgacgtacg tgatgaccct cgcgggcgtc
     6241 ctggcggccc cggccctgct gatcccgggc atccccgggg cgctcctcgg cgccctcatg
     6301 gtccagctct acctgctgct cgactgcgtc gacggcgagg tggcccgctg gaagaagcag
     6361 ttctcgctcg gtggcgtgta cctggaccgg gtcggtgcct acctgtgcga cgcggcggtg
     6421 ctggtcggtt tcggcctgcg cgccgccgac ctgtggggca gcgggcggat cgactggctg
     6481 tgggccttcc tcggcaccct cgccgccctc ggcgccatcc tgatcaaggc cgagaccgac
     6541 ctcgtcggcg tcgcccgcca ccagggcggc ctgccgccgg tgaaggagtc ggcctccgag
     6601 ccgcgctcgt ccggcatggc gctggcccgc aaggccgccg cggcgctgaa gttccaccgg
     6661 ctcgtcctgg gcatcgaggc gtcgctgctc atcgtggtgc tggcggtcct ggacacggtc
     6721 cggggcgacc tgttcttctc ccgtctcggt gtcgcggtcc tggccggcat cgcgctgctc
     6781 cagaccgtgc tgcacctcgt ctcgatcctc gtgtcgagca ggctcaagtg aggacgccga
     6841 tgaagctggg cgccgtcatc atcaccatgg gcaaccgccc cgacgacctc cgcgcgctga
     6901 tcgaatcggt cgccgagcag gacggcgacc cgatccaggt cgtcgtcgtc ggcaacggcg
     6961 ccccggtgac cggtgtcccc gagggcgtac gcaccatcga cctcccggag aacctgggca
     7021 tccccggcgg ccgcaacgtc ggcatcgagg cgttcggccc cggcggcacc gacgtggacg
     7081 ccctgctgtt cctggacgac gacgggctgc tggcgaacac ggacaccgcc gagctgatcc
     7141 gccaggcgtt caccgccgac ccggagctgg gcatcatcag cttccggatc gccgacccgg
     7201 acaccggcgt cacccagcgc cggcacgtgc cgaggctgcg ggcctcagat ccgatgcgct
     7261 cctcgcgggt gacgaccttc ctcggcggcg ccaacgccgt ccgtacgaag gtgctggccg
     7321 aggtcggcgg gctgccggac gagttcttct acgcgcacga ggagaccgac ctcgcctggc
     7381 gggcgctcga cgccggctgg atgatcgact accgcgcgga catggtgctc ttccacccga
     7441 cgaccgcgcc gtcccggcac gccgtctacc accgcatgat cgcccgcaac cgcgtctggc
     7501 tggcccgccg caacctcccc gcgcccctgg tgccgctcta cctcggcgtc tggctgctgc
     7561 tcacgctggc ccgccgccct tcggtgccgg cgctgaaggc ctggttcggc gggttcaagg
     7621 agggctggac gaccccgtgc gggccgcgcc ggcccatgaa gtggcgtacg gtgtggcgcc
     7681 tgacgagact gggccgccca cccgtcgtct gacaagctcg gaatctgaca agctcggacc
     7741 gacaagcacg gggcatcccg gggcccggcc gccacctgac ccgcccgccc ctcgactgcc
     7801 gcgcatcctg aacacgaaag tttccccttg tgagtgagac aacccatgac ggagcgatcg
     7861 ccgtgaccgc cccgccatca ccggacgcgg gcctgtcccc ggcggagctg gccgccaagt
     7921 acggcctgtc ggtcagcggt gcccggcccg gtctggccga gtacgtccgg cagctgtggg
     7981 ggcgccgtca cttcatcctg gccttctccc aggcgaagct cacggcccag tacagccagg
     8041 ccaagctcgg ccagctctgg caggtggcga caccgctgct caacgcggcg gtctacttcc
     8101 tcatcttcgg cctgatcctg aacgccgggc ggggcatgga gaaggaggtc tacatcccgt
     8161 tcctggtgac gggtgtcttc gtcttcatgt tcacgcagac gtccgtgatg tccggcgtac
     8221 gggcgatctc ggggaacctg ggcctggtgc gggcgctgca cttcccgcgc gcctccctgc
     8281 ccatctcctt cgcgctccag cagctccagc agctgatgtt ctcgatgatc gtgctgttcg
     8341 tgatcgcggc ggcgttcggc agctacccct cgctgagctg gctgctgatc ctcccggcgc
     8401 tggcgctgca gttcctcttc aacgtcggcc tgtcgctgat catggcgcgg ctcggcagca
     8461 agacccccga cctcgcgcag ctgatgccct tcgtcatgcg cacctggatg tacggctcgg
     8521 gcgtcatgtt ctcgatcccg atcatgctca aggacaagcc ggcctggatc gcggacgtcc
     8581 tgatgtacaa cccggcggcg gtctacatgg acctcatccg gttcgcgctg atcgacggat
     8641 acggttcgca gaacctgccc tcgcacgtct ggctcgccgc cctgctgtgg gcggtcctgg
     8701 tcggggccgc cgggttcgtg tacttctgga aggcagagga gcggtacggc cgtggctgag
     8761 cagaccgacg acgtgaccgc gaccgccgcc gccgacggcg cgcggatccc caccgtcatc
     8821 gccgacgacg tgcacatcgt gtaccgggtc aacggcggcg gcggaggcaa gggcagcgcc
     8881 accgccgcgc tcagccggat gatccgccgg gacaagggcg agtccagggg cgtacgcaag
     8941 gtgcacgccg tgcgcggggt gtccttcacc gcgtaccggg gcgaggccat cggcctcatc
     9001 ggcaccaacg gctccgggaa gtcgaccctg ctgcgggcca tcgcgggcct gctgcccacg
     9061 gagaagggca aggtctacac ggacggccag ccctcgctgc tgggcgtcaa cgcggcgctg
     9121 atgggcgacc tgaccggtga gcgcaatgtg atcctcggcg gactggccat gggcatgtcg
     9181 cgcgaggaga tccgggagcg gtaccaggag atcgtcgact tctccggcat caacgagaag
     9241 ggcgacttca tcaccctccc catgcggacg tactcctccg gtatgggcgc ccggctccgg
     9301 ttcgccatcg ccgccgcgaa gaaccacgac gtcctgatga tcgacgaggc gctcgccacc
     9361 ggtgaccgca agttccagat ccgctccgag cagcgcatcc gcgagctgcg caaggaggcc
     9421 ggcacggtct tcctggtcag ccacagcaac aagtcgatca gggacacctg cgaccgcgtc
     9481 ctgtggctgg agaagggcga gctgctgatg gacggcccca cggacgaggt cctgagggcg
     9541 tacgagaagg agaccggcag gtagtccggc cgggatccgt acgagggcgg ctgtacgaag
     9601 ccgggaaggg cccctgccgg agcgcacggc aggggccctt ccggtggcgg gcacgcgccg
     9661 gtcgtaccaa ccgccccggg atgccatgtt tcggtcaagt cccgtgcggc gcggggcggt
     9721 tgacgtcaag atcgtgaccg ttcccgggtg tgtgacaccc cggcacggcc cgggacgttg
     9781 tacaacgtaa gctgtaccgg tcctgattcg cggcaagtgg ggcgatattc cccggccccc
     9841 accgggccgg ggcggtgcct cgcaggccag gaccggcggc gtgtccgaaa tgagatgtat
     9901 tgggtcggca gtgtagaacg ggagacgtga cggcaatgac ggtgaatccc cagccccgcc
     9961 tccgcaaggg tcccgccgtc cccgcgccgg gcagtccgca gtgacaggta cccggcaggt
    10021 gcgccccgac gcctccccgc gcaccacact cgacaaggcc gcgcacgaga acttccccgt
    10081 ggcccccttc ttcctgcccc gtgcctggcg cgacgacctc atggccgtct acggctacgc
    10141 ccgcctcgtc gacgacatcg gcgacggcga cctcgccccg ggcggcgccg acgcccgcca
    10201 tctgggcgcc gagggggaga cggaccggct cgccctgctc gacgccttcg aggcggacct
    10261 gcggcgcgtc ttcgacggca ccccgtccca cccgctgctg cgcgccctga agaccaccgt
    10321 gcggcgccac gcgctcaccc ccgagccgtt cctgggcctg atcgaggcca accgccagga
    10381 ccaggtggtg cggcgctacc agacgtacga cgagctcgtc gcctactgcg agctgtccgc
    10441 caaccccgtc gggcgcctcg tcctggacat caccggcacc accagccccg aacgcctgcg
    10501 ccgctccgac gccgtctgca ccgccctgca gatcgtcgag cacctccagg acgtcgccga
    10561 ggacctcgcc cacgaccgga tctacctccc ggccgaggac atgaaacgtt tccacgtcac
    10621 cgaggccgac ctggcccgcc ccaccggcgg cgcctcggta cgcgccctca tcgcgtacga
    10681 ggccgaacgc gcccgccggc tcctggacga cggcatcccc ctcgtgggca gcgtccgcgg
    10741 caggctccgg ctcctcctcg ccggcttcgt cgccggaggg cacgccgccc tcgacgcgat
    10801 cacggccgcc ggacacgacg tactccccgg accgcccaag gccaccgggc ccaaactgat
    10861 gcgcgaagtg ggaaccgtgt tgcgcagagc gcgtagagag gggtgagcgg gaccgtggag
    10921 ggacagacgg agatgtccgc accggtggcg gccgcctaca gctactgcga agccgtcacc
    10981 ggacggcagg cccgtaactt cgcctacggc atccggctcc tgccgaccgg gaagcggcag
    11041 gccatgtcag cgctgtacgc gttctcccgg cgggtcgacg acatcggcga cggcacgctc
    11101 gcccccgagg cgaagcagga gcggctggaa accacccgtg agctgctgag gcggatccgc
    11161 gagggcgccg tcggccagga cgacaccgac ccggtggccg tcgcgctcgc cgacgccgca
    11221 cgccgcttcc cgctgccgct cggcggcctc gacgagctga tcgacggcgt cctgatggac
    11281 gtgcgcggcg agacgtacga gacctgggac gacctcaagg tctactgccg gtgcgtcgcc
    11341 ggcgccatcg gcaggctctc cctctccatc ttcggcacca cacccggcgc acgcggcgcg
    11401 gcacgcgccc ccgagtacgc cgatacactc ggcctcgccc tccaactcac caacatcctc
    11461 cgcgacgttc gcgaggacgc cggcaccgga cgcacctacc tgcccgccga cgacctcgcc
    11521 aagttcggct gctccgacgg cttccgcacc gccaccccgc cccccggctc cgacttcacc
    11581 ggcctcgtcc actacgaagt ccggcgcgcc cgcgccctgt tcgccgaggg ctaccggctg
    11641 ctgcccatgc tcgaccggcg cagcggcgcc tgcgtcgccg ccatggccgg catctaccgc
    11701 cgcctcctcg accgcatcga gcgcgacccc gaggccgtcc tgcgcggccg tgtcagcctg
    11761 cccggacacg agaaggcgta cgtcgccgtg cgcggcctgt ccggcctgga cgcccgtacc
    11821 gtctcccggc agaccgtcag gaggcgcgcc tagatggcca ccatgccagc gacccggccc
    11881 gcgaaccggc acaatcagcg cgcaaccctc cgccacagcc tcgcgtccct gactgcgacg
    11941 acccgacggg agaccggatg agcgacgccg acctgacacc gggccacgcc gccccggggc
    12001 acgccgtggt cgtcggcggc ggcctcgccg gcatcaccgc cgccctgcgc ctggccgact
    12061 ccggactgcg cgtcacactc ctcgaaggcc ggccccgcct cggcggcctc gccttctcct
    12121 tcaagcgcgg cgacctcacc gtcgacaacg gccagcacgt ctacctccgc tgctgcaccg
    12181 cctaccgctg gttcctcgac cgcgtcgacg gcgcccacct cgcgcccctc cagccccgcc
    12241 tcgacgtgcc cgtcctcgac gtcgcccacc cgcgcggccc ccgcctcggc cggctgcgcc
    12301 gcaccgccct gcccgtaccg ctgcacctcg ccgcgagcct cgccacgtac ccgcacctct
    12361 cgctcgccga gcgggcgagc gtcggccgcg ccgccctcgc cctgaagcgg ctcgacccgg
    12421 ccgaccccgc cctcgacggc atcgacttcg ccacctggct cgcccgccag ggccagtccg
    12481 cgcgcgccac cgaagccctg tgggacctga tcggcgtcgc caccctcaac gccaccgccc
    12541 cgcactcctc cctggcgctg gccgccatgg tcttcaagac cgccctcctg accgaccccg
    12601 gcgccgccga catcggctgg gcgcacgtcc cgctcggcga cctgcacgac accctcgccc
    12661 gcaaggcgct cgacgccgcc ggcgtacgca ccgagctgcg cgccaaggtc agcgccatcc
    12721 tgccgagcgc ccacggccgc tgggccgtcg acgtacccgg cgaacggctg gaggccgaca
    12781 ccgtcgtcct cgccgtaccg cagcgcgagg cccacgacct gctgccgcac ggggcgctgg
    12841 acgaccccgg cacgctcctc gacatcggca ccgccccgat cctcaacgtc cacgtcctgt
    12901 acgaccgcaa ggtgctgcgc cgcccgttct tcgccgcgct cggctccccg gtccagtggg
    12961 tcttcgaccg caccgacgcc tccggctaca ccgacggcca gtacctggcc ctctcccagt
    13021 cggccgccgg ggacgagatc gacgagcccg tctccgtact ccgcgagcgc tacctgcccg
    13081 agctggagcg gctgctgccc gcggcacgcg gcgcgaaggt ccgggacttc ttcgtcaccc
    13141 gggagcggac ggcgacgttc gcccccaccc ccggcgtcgg ccggctgcgg cccggcgccc
    13201 gcaccaacgc gcccggcgtg cacctggccg gcgcgtggac cgccaccggc tggcccgcga
    13261 ccatggaggg cgccgtacgc agcggcttca ccgccgcggg cgcggccctc tcggccctcg
    13321 gccgccccca tgtacatccg ctcgaggagg cggcatgagc ctgagtactg gaaccagagg
    13381 agagaccgtg ccgactgtgc ccccggtgaa cccggccgtc gacaccgcgg acgtcaccgc
    13441 actgctggag cggggccgga ccatgtccac ccccgtcctg cgcacggcgg tgaagcggct
    13501 ggcgccgccc atggacaccg tcgccgctta ccacttcggc tggatcgacg cccacggccg
    13561 gcccgccgac ggcgacagcg gcaaggccgt acgccccgcc ctcgccctgc tgtccgccga
    13621 ggcggccggt gtggcgccgg aggtcggcat ccccggcgcg gtcgcggtgg agctggtgca
    13681 caacttctcg ctgctgcacg acgacctgat ggacggtgat gaacagcgcc gtcaccgcga
    13741 caccgtgtgg aaggtgcacg gccccgccca ggccatcctc gtcggcgacg ccctgttcgc
    13801 gctcgccaac gagctgctgc tggagctcgg caccgtcgag gccggccgcg ccacccgccg
    13861 gctgaccacc gccacccgca agctcatcga cggccaggcc caggacatct cctacgagca
    13921 ccgagagcgg gtcaccgtcg aggagtgcct ggagatggag ggcaacaaga ccggcgcgct
    13981 gctcgcctgc gcctgctcca tcggcgccgt cctcggcggc gccgacgacc gcaccgccga
    14041 caccctggag gcgtacggct accacctcgg cctcgccttc caggccgtcg acgacctgct
    14101 cggcatctgg ggcgacccgg aggccaccgg caagcagacc tggagcgacc tgcgccagcg
    14161 caagaagtcc ctgccggtcg tcgccgcgct cgccgccggc ggaccggcct ccgagcgcct
    14221 cggggagctg ctcgcggccg acgccaagag cagcgacctc gacagcttct ccgaggagga
    14281 gttcgccatg cgcgccgccc tcatcgagga ggcgggcggc cgcgcgtgga cctccgagga
    14341 ggcccgccgg cagcacgccg tcgcgatcga ggcgctgcac cgcgtcgaca tgccggaacg
    14401 ggtccgcgcc cagctcaccg cgctcgcgga cttcgtcgtc gtacgaaagc gatgaccgcc
    14461 atcgggacga gcacccgcac catatgaatc gctagtcgcc ggccggtgca ctccgctgta
    14521 ccggccgacg gcccacccgc agccgcagaa cgccaactgc acgaagggga agccatgaca
    14581 gcgacgaccg acggaagcac cggggccctg gagccccgcg ccgcctcggc caccggaacc
    14641 accaccggaa cgaccaccac caccacgacc cccgcagcga acgacgtcag ggaagtcgcc
    14701 gcgcgggcag cccaacgctc catcgagtac ctcctggccc ggcaggacga cgagggctgg
    14761 tggaagggcg acctggagac caacgtcacc atggacgccg aggacctcct gctgcggcag
    14821 ttcctcggca tccaggacga ggcgaccacc cgcgccgccg ccctccacat ccgcggcgaa
    14881 cagcgcgacg acggcacctg ggcgaccttc tacggcggcc ccggcgagct gtccaccacc
    14941 atcgaggcgt acgtcgcgct gcgcctggcc ggcgaccggc ccgacgagcc gcacatggcc
    15001 cgtgccgccg cctggatccg cgagcagggc ggcatcgccg ccgcccgcgt gttcacccgc
    15061 atctggctgg ccctgttcgg ctggtggaag tgggaggacc tgcccgagct gccgccggag
    15121 ctggtcttcc tgccgaagtg gttcccgctc aacatctacg acttcggctg ctgggcccgg
    15181 cagaccatcg tgccgctcac cgtcgtctcc gccaagcggc ccgtccgccc cgcgtccttc
    15241 ccgctcgacg agcttcacac cgacccccgg ctcccgaatc cgccgaggac cctcgctccc
    15301 gccgggacct gggagggcgc cttccagcgg ctggacaagg cgctgcacgc gtaccaccag
    15361 gtcgcgcccc ggtggctgcg caaggccgcg atgaacaccg ccgcccgctg gatcatcgaa
    15421 cggcaggaga acgacggctg ctggggcggc atccagcccc ccgccgtcta ctccctcatc
    15481 gcgctccacc tgctcggcta cgacctggag cacccggtga tgcgcgcggg cctggagtcc
    15541 ctcgaccggt tcgccgtgtg gcgcgacgac ggcgcccgca tgatcgaggc ctgccagtcg
    15601 cccgtctggg acacctgcct ggccaccatc gcgctcgccg acgccggcgt acgccccgac
    15661 cacccggcgc tggtcaaggc cgccgactgg atgctgggcg aggagatcgt ccggcccggc
    15721 gactgggccg tccagcggcc cggactcacc cccggcggct gggcgttcga gttccacaac
    15781 gacaactacc cggacatcga cgacaccgcc gaggtcatcc tggcgctgcg ccgggtcaag
    15841 cacccccacc cggagcagat cgaggcggcg atccggcgcg gcgccaactg gaccgccggc
    15901 atgcagtgcc gcgacggcgc ctgggccgcc ttcgacgccg acaacaccag ccccttcccc
    15961 aacctgctgc cgttctgcga cttcggcgag gtcatcgacc cgccgtccgc cgacgtcacc
    16021 gcgcacgtgg tggagatgct ggcccacgag ggcaaggccc accatccccg tgcccgccgc
    16081 ggaatcacct ggctgctggc cgaacaggag ccgagcggcg cctggttcgg gcgctggggc
    16141 gtcaactacg tctacggcac cggctcggtg gtgcccgccc tcaccgcggc ggggcttccc
    16201 ccgtcgcacc cggccatccg gcgcgccgtc gcctggctgg agtcggtcca gaacgacgac
    16261 gggggctggg gcgaggacct gcgctcgtac cgggagcggg agtggatcgg ccacggtgcc
    16321 tccacggcct cgcagacggc gtgggcgctg ctggcgctgc tggcgggcgg gcagcgggag
    16381 tccagggccg tggagcgcgg cgtccgctgg ctcgccggga cccagcggga ggacggctcc
    16441 tgggacgagc cgtacttcac cgggaccggc ttcccctggg acttctccat caactaccac
    16501 ctctaccggc aggtcttccc gctgaccgcg ctcggccggt acgtcaacgg cgaaccggtg
    16561 gtcggccggc tcggcaaggg ggcctgatgg cggcagaacc ggagccttcc ggttccgctc
    16621 acgcttccgc ggcggctcct gtgccgccca tgctgatcgc ctgcgcgctc ggcatcgagc
    16681 agctcgcgct gcgcagcggc cgccgccgcc acggcggtac gtcgcccggc ccggtgaccg
    16741 tgctgcgtac gggcatgggg cccgcgaacg cccagcgggc cgtcacccgg gcgctggagg
    16801 gcggcgagct gcgcggcgcc gcggtcgtcg cgtccgggtt ctgcgcgggg ctggagccgg
    16861 gcatgcaccc gggagacctg gtcgtcgccg acgagacccg gggccccggc ggctccaccc
    16921 tgtgcacggg cacggacctg ctggtggacg cgctgacccg ggccgttccc ggccggaccg
    16981 tccacagcgg cccgctgacc ggctccgacc acgtggtacg cggtcaggaa cgggcccagc
    17041 tgcgcggcac cggggcgatc gcggtcgaca tggagtccgc ggccaccctc cgctccgcgc
    17101 tgaacacggg cgtccggtcc cgcccggttg cggccgtacg agtggtcgtg gacgctccag
    17161 agcacgaact cgtccggatc ggcacggtac gcgggggaat atcagccttc cgtgttcttc
    17221 gttctgtatt gcccgctttc tatgaatggc accgttcttt gctgctcccc aggaggtgag
    17281 ccagatggcc atgccgcttc gccagaccat ccgggtaggg acgtatctct tcgaacagaa
    17341 gctgcgcaag cgtgagaagt tcccgctcat cgtcgaactg gagccgctct acgcctgcaa
    17401 cctcaaatgc gagggctgcg ggaagatcca gcaccccgcc ggggtcctca agcagcgcat
    17461 gccggtcgcc caggcggtcg gagcggtcct ggagtccggc gcgccgatgg tctccatcgc
    17521 gggcggcgag ccactgatgc acccgcagat ccacgagatc gtgcgccagt tggtggccaa
    17581 gaggaagtac gtcttcctct gcaccaacgc cctcctgctg cgcaagaaga tcgaggactt
    17641 cacgccgtcg ccgtacttcg ccttcgccgt gcacatcgac ggcctgcgcg aacggcatga
    17701 cgagtcggtc gccaaggagg gcgtgttcga cgaggcggtg gaggccatca aggaagccaa
    17761 gcggcggggc ttccgggtca ccaccaactc caccttcttc aacaccgaca ccgcgcagac
    17821 cgtcatcgag gtgctcaact tcctcaacga cgacctgaag gtcgacgaga tgatgatctc
    17881 gcccgcgtac gcgtacgaga aggcgcccga ccaggagcac ttcctgggcg tcgagcagac
    17941 ccgcgagctg ttccggaagg ccttctcggg cggcaaccgg cggcgctggc ggctgaacca
    18001 ctcgccgctc ttcctggact tcctggaggg caaggccgac ttcccgtgca ccgcctgggc
    18061 gatccccaac tactcgctct tcggctggca gcggccctgc tacctgatgg ccgacgggta
    18121 cgtcccgacg taccggcagc tcatcgagga gaccgactgg gacaagtacg gccggggcaa
    18181 ggacccgcgc tgcgccaact gcatggcgca ctgcggctac gagccgaccg ccgtcctcgc
    18241 caccatgggc tcgctcaagg agtccctgcg cgccgcccgc gagacggtct ccggaagccg
    18301 ggggtgaggt gatgccggag cctgtgggcc tcgggctgcc cgagctgccg ccccggccga
    18361 tcgcggtacg ccgcccctcg cggcgcatcc aggtcgggtc ggtggcggtc ggcggcgacg
    18421 cgcccgtgtc ggtgcagtcc atgacgacga cccggacctc cgacgtcggc gccacgttgc
    18481 agcagatcgc ggagctgacc gcgtccggct gccagatcgt acgggtggcg tgccccaccc
    18541 aggacgacgc ggacgcgctg gcggtcatcg cgaagaagtc gcagatcccg gtgatcgcgg
    18601 acatccactt ccagccgaag tacgtcttcg ccgccatcga cgcgggctgc gcggcggt
//
