LOCUS       NZ_LIYH01000005        10393 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB03234 scaffold5, whole genome shotgun sequence.
ACCESSION   NZ_LIYH01000005
VERSION     NZ_LIYH01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB03234
  ORGANISM  Streptomyces sp. CB03234
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 912609)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912609)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905885.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:52:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,127
            CDSs (total)                      :: 7,053
            Genes (coding)                    :: 6,894
            CDSs (with protein)               :: 6,894
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 70 of 159
            Pseudo Genes (incomplete)         :: 112 of 159
            Pseudo Genes (internal stop)      :: 10 of 159
            Pseudo Genes (multiple problems)  :: 33 of 159
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 789380
            Orig. end                         :: 799773
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIYH01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="4"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5393
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            1145..2683
                     /locus_tag="AMK26_RS25005"
                     /old_locus_tag="AMK26_24630"
     CDS             1145..2683
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (102-484). E-value:
                     8.2e-29. Score: 92.9. Matches aSDomain:
                     nrpspksdomains_AMK26_RS25005_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018520408.1"
                     /locus_tag="AMK26_RS25005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_24630"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_073756933.1"
                     /sec_met_domain="PF00561 (E-value: 2.4e-40, bitscore:
                     134.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDSRRLLRTSAVALSLLGLLMAGCTDGGSTPAASAPASASVASTE
                     ELRAYYGQRLTWRACGAPGFECATMKAPLDYAKPRGEQIKLAVSRFKATGPGKRLGSLL
                     VNPGGPGGSAVGYLQGYAGLGYPAPVRARYDIVAVDPRGVARSEPVRCLDGRQMDAYTQ
                     VDQTPDDRDEIARLAASFKNFAAGCQKLSGRILPHVSTVESARDMDILREVLGDRRLTY
                     VGASYGTFLGATYAELFPTRAGRLVLDGAMDPSLPTAELNRDQTAGFETAFRSFAADCV
                     KKTDCPLGTESPTHAAARLKAFFAQLDAEPVPTGESRRLGEALATTGVIAAMYDESAWP
                     QLREALTRAMGGEGSALLALADSYYEREEDGTYSNLMYANAAVNCLDQPPAFTGPDQVA
                     AAVPSFEKASPVFGAGLAWASLNCAYWPTKATGTAHRIHADGAPPILVVGTTRDPATPY
                     KWAESLAAQLSSGRLLTYEGDGHTAYGRGSDCIDTAINTFLLDGTVPPSGKRCR"
     aSDomain        1451..2596
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK26_RS25005_Abhydrolase_1.1"
                     /evalue="8.20E-29"
                     /label="AMK26_RS25005_Abhydrolase_1.1"
                     /locus_tag="AMK26_RS25005"
                     /protein_end="484"
                     /protein_start="102"
                     /score="92.9"
                     /tool="antismash"
                     /translation="LLVNPGGPGGSAVGYLQGYAGLGYPAPVRARYDIVAVDPRGVARS
                     EPVRCLDGRQMDAYTQVDQTPDDRDEIARLAASFKNFAAGCQKLSGRILPHVSTVESAR
                     DMDILREVLGDRRLTYVGASYGTFLGATYAELFPTRAGRLVLDGAMDPSLPTAELNRDQ
                     TAGFETAFRSFAADCVKKTDCPLGTESPTHAAARLKAFFAQLDAEPVPTGESRRLGEAL
                     ATTGVIAAMYDESAWPQLREALTRAMGGEGSALLALADSYYEREEDGTYSNLMYANAAV
                     NCLDQPPAFTGPDQVAAAVPSFEKASPVFGAGLAWASLNCAYWPTKATGTAHRIHADGA
                     PPILVVGTTRDPATPYKWAESLAAQLSSGRLLTYEGDGHTAY"
     tRNA            2781..2854
                     /anticodon=(pos:792195..792197,aa:Thr,seq:cgt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="AMK26_RS25010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="AMK26_24635"
                     /product="tRNA-Thr"
     gene            2781..2854
                     /locus_tag="AMK26_RS25010"
                     /old_locus_tag="AMK26_24635"
     gene            complement(2975..3277)
                     /locus_tag="AMK26_RS25015"
                     /old_locus_tag="AMK26_24640"
     CDS             complement(2975..3277)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMK26_RS25015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AMK26_24640"
                     /product="hypothetical protein"
                     /protein_id="WP_073756934.1"
                     /transl_table=11
                     /translation="MDTEPTVGIRIIRRALGLAFLAFFLIASVVAGSFAGWGLGERTTV
                     ERIQDAVPSLLMSLTWGAVAIAGFRLLLGHRLLSRWLACALVIPASAALHAADVL"
     misc_feature    3435..3449
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.24"
                     /tool="antismash"
     gene            complement(3540..4184)
                     /locus_tag="AMK26_RS25020"
                     /old_locus_tag="AMK26_24645"
     CDS             complement(3540..4184)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019884846.1"
                     /locus_tag="AMK26_RS25020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_24645"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_241838050.1"
                     /transl_table=11
                     /translation="MTMTRVEGEAPLARDEVAEERDTTVGAGRAFAWMLVATGAVGLLA
                     AWVITLDKFRLLEDPNFTPGCSLNPVVSCGNIMKSEQASVFGFPNPMLGLVTYGMVIAI
                     GAALLSGARLRRWYWLGLNAGTLFGVGFCTWLMHQSLYTINSLCLWCCSAWVATIVMFW
                     YVTSHTVRKRLLPAPVAVRGFFGEFTWVLPVLHIGIIGMLILTRWWDFWTS"
     gene            complement(4313..4822)
                     /locus_tag="AMK26_RS25025"
                     /old_locus_tag="AMK26_24650"
     CDS             complement(4313..4822)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889043.1"
                     /locus_tag="AMK26_RS25025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_24650"
                     /product="DUF5949 family protein"
                     /protein_id="WP_073756935.1"
                     /transl_table=11
                     /translation="MTSSTAVTPGRQESLLGTLAVIAWASKPESDADSPVTPFLMVYSL
                     GDGRDGPEAGADALRSKLEETGLSIGDKVTDLSRETRIPITLLVEAEQAVLNLPFMKVQ
                     CPVQPEWEKAAHESGTAYLICALLPWPEAVQGKSVSEEELLAFLGDDDLVAKSAHCLLP
                     VRRVRT"
     gene            5001..5393
                     /locus_tag="AMK26_RS25030"
                     /old_locus_tag="AMK26_24655"
     CDS             5001..5393
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889042.1"
                     /locus_tag="AMK26_RS25030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_24655"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_073756936.1"
                     /sec_met_domain="melC (E-value: 3e-46, bitscore: 152.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIARPPLPRLLPRRRAVLRALFTLTVTAFTGGSLAHVATTPSAGA
                     ARRSTGESDEMYRGRHIQVRPVAGSRFDVRVDGRPLHLMRCADGGYLTLVDHYQSYPTP
                     LAAARAAVDELGSAQLAPGAHHGVRT"
     gene            5377..6243
                     /locus_tag="AMK26_RS25035"
                     /old_locus_tag="AMK26_24660"
     CDS             5377..6243
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071025.1"
                     /locus_tag="AMK26_RS25035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_24660"
                     /product="tyrosinase family protein"
                     /protein_id="WP_073756937.1"
                     /transl_table=11
                     /translation="MAYVRKNQRDLTGAERKKFVSAVLALKRSGRYDDFVRTHIRYYTA
                     DGDDDLRVAHMAPSFLPWHRQYLLEFERALRSVDPTVTVPYWDWTRDNTPAASLWGEDF
                     MGGNGRRGDRQVMTGPFAYAKGNWPITVGVTESPFLTRNFGNPANPIDLPTRQELARAT
                     AETVYDSAPWDSTSRTGFRNALEGWTRESGNGRWRNHNRVHRWVGGLMLGGASVNDPVF
                     WLHHSFIDLIWSRWQSKNPRAGYLPATPPPPGSAQHRRVVALDERMPPFEVTPADVADH
                     HRVYRYA"
     gene            complement(6308..6580)
                     /locus_tag="AMK26_RS25040"
                     /old_locus_tag="AMK26_24665"
     CDS             complement(6308..6580)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449724.1"
                     /locus_tag="AMK26_RS25040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_24665"
                     /product="chaplin"
                     /protein_id="WP_073756938.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVIVGTGAVVLSGSGMAVAGADADAAVVESPGVISGN
                     AVQVPIDIPINLCGNTIDIIGLLNPAFGNQCANVQHKDHKGGYGG"
     gene            complement(6772..7293)
                     /locus_tag="AMK26_RS25045"
                     /old_locus_tag="AMK26_24670"
     CDS             complement(6772..7293)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035837.1"
                     /locus_tag="AMK26_RS25045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_24670"
                     /product="hypothetical protein"
                     /protein_id="WP_073756939.1"
                     /transl_table=11
                     /translation="MNLKSQASMGFVVTCLASAAMASPAAAAATVPVAPVDVPLHGLGT
                     VLPETPTLRTGVPIPLTGAPMGLTAHGKGQAQPVLLPQLPVATGVPETLVSAPLPNVVE
                     GEPTGRALISAPASAIHATTPGAILGLPITLPHAESLGLPGLKLPEAGFLAPTVTGALD
                     STLGLAQQEN"
     misc_feature    complement(7335..7344)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.82"
                     /tool="antismash"
     gene            complement(7556..7837)
                     /locus_tag="AMK26_RS25050"
                     /old_locus_tag="AMK26_24675"
     CDS             complement(7556..7837)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889038.1"
                     /locus_tag="AMK26_RS25050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_24675"
                     /product="hypothetical protein"
                     /protein_id="WP_073756940.1"
                     /transl_table=11
                     /translation="MKPTKVAAVVAGSVMALGAAAPAFAVDSLTPSSLNGGVEALGKRG
                     LTEAVPTDAVGKVTNGKAVDKVQDAAGGLEPAKDTAQPLLGGLPALGK"
     gene            complement(8138..8776)
                     /locus_tag="AMK26_RS25055"
                     /old_locus_tag="AMK26_24680"
     CDS             complement(8138..8776)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMK26_RS25055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AMK26_24680"
                     /product="hypothetical protein"
                     /protein_id="WP_073756941.1"
                     /transl_table=11
                     /translation="MRLLTPRRVAVTALCAALTLGTAGPAIAHSTHQPAKEQQAKAPED
                     ISDILGSIGSVLDSVLGTLSGTSSAQTLPATDMSQQMKDLTAAIEALKKAVEEKNTAAT
                     GTGTGTGTGMTADDISMDIVDTDTTSDTTMDGDLDKALATMDKAIDDMVAAAVNGQGAT
                     SASQIQAATKNLVDTIMRDVAKMTGTPTTGMNPLPAVTLPATTLPATGL"
     misc_feature    8839..8852
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.43"
                     /tool="antismash"
     misc_feature    8972..8987
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.33"
                     /tool="antismash"
     gene            complement(9054..9293)
                     /locus_tag="AMK26_RS25060"
                     /old_locus_tag="AMK26_24685"
     CDS             complement(9054..9293)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="AMK26_RS25060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_24685"
                     /product="chaplin"
                     /protein_id="WP_073756942.1"
                     /transl_table=11
                     /translation="MSRIAKAAVLTSAVAASVAGATGVAHADADAEAAAVKSPGVITGN
                     VIQVPIHIPINLCGNTIDIIGALNPTFGNVCIND"
     misc_feature    complement(9430..9445)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.89"
                     /tool="antismash"
     misc_feature    9489..9498
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.11"
                     /tool="antismash"
     misc_feature    complement(9492..9508)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.19"
                     /tool="antismash"
     misc_feature    9500..9516
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.89"
                     /tool="antismash"
     misc_feature    9780..9795
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
ORIGIN
        1 acgcgtcgaa gatgacgcac gcgtggctgt tcaccggccc gccgggctcc gggcgcacca
       61 ccgccgcccg cgccttcgcg gccgccctcc agtgcgtcag ccccgaccgg gcgctgggcg
      121 gcgaaccggg ctgcgggttc tgcgacgggt gccacaccgc gctgatcggc acccacgccg
      181 acgtgtccac ggtcgccgcc gtgggcaccc agatcttggt cgacgacatg cgcgacacgg
      241 tccgcaagtc gttcacgtca cccgcgaacg gccgctggca ggtcatcctc gtcgaggacg
      301 ccgagcgcct caacgagaag tccgccaacg ccgtcctgaa ggcggtggag gagcccgctc
      361 cccgcacggt gtggatgctg tgcgcgccgt ccatcgagga cgtcctgccc accatccgct
      421 cccgctgccg ccacctcacg ctgcgtacgc cgccggtcga ggccgtcgcg gacgtcctga
      481 tccggcgcga cggcatcgag ccggaggccg cgtacgccgc cgcccgcgcc acccagggcc
      541 acatcgacca cgcgcgccgc ctcgccaccg acgagcgcgc ccgggcccgc cgggccacgg
      601 tcctcaagct gccgctgcgg gtggaggaca tcggtggttg cctcaaggcg gcgcaggagc
      661 tgatcgacgc ggcggccgag gacgccaagg aggtcgccga gggcaccgac gtcaaggaga
      721 ccgaggagct gaaggccgcc ctgggcggta cccccggtgg caggatgccg cgcggcacgg
      781 cgggcgccat gaaggagctg gaggacaagc agaagcgccg caggacccgc acgcagcgcg
      841 acagtctgga cctggccctc ggggagctga cgagcttcta ccgcgacgtc ctggcgctcc
      901 agctcggcgc gggcacggcc ctggccaatg aagacgtgcg tgaggcgctg gaccggacgg
      961 cccgcgagtc gacaccggag cggaccctgc gccggatcga ggcggtcctc gcctgccgcg
     1021 aggccatgga ccgcaatgtg gcgccgttgc tcgcggtgga ggccatgacc atggcgctgc
     1081 gcgcggggta atccgtacgg gtagtcgcga ccacaagcgg tgaacgtccg gatacgctcc
     1141 ggtcatggac tcccgccgcc tcctccgcac gtccgccgtc gccctctcgc tcctcggcct
     1201 gctcatggcc ggttgcacgg acgggggttc gaccccggcc gcctccgccc cggcctcggc
     1261 ctcggtcgcc tcgaccgagg agctcagggc gtactacggc caacggctga cctggcgcgc
     1321 atgcggcgcc cccggtttcg agtgcgccac catgaaggcc ccgctcgact acgccaagcc
     1381 gcgcggcgag cagatcaagc tggccgtgtc ccggttcaag gccaccggcc ccggcaagcg
     1441 cctcggctcg ctcctggtca acccgggcgg cccgggtggc tcggccgtcg gctacctcca
     1501 ggggtacgcg ggcctcggct accccgcccc cgtccgcgcc cgctacgaca tcgtcgcggt
     1561 cgacccgcgc ggggtggccc gcagcgagcc cgtccggtgc ctcgacggcc ggcagatgga
     1621 cgcgtacacg caggtcgacc agacccccga cgaccgtgac gagatcgccc gcctcgccgc
     1681 ctccttcaag aacttcgcgg ccggctgcca gaagctctcc ggccggatcc tgccccacgt
     1741 atccacggtc gagtcggccc gcgacatgga catcctccgc gaagtgctgg gcgacaggcg
     1801 gctcacctat gtgggcgcct cgtacggcac gttcctcggg gcgacgtacg ccgaactctt
     1861 ccccacccgc gccggccggc tcgtcctgga cggggccatg gacccgtccc tgccgaccgc
     1921 cgagctgaac cgcgaccaga cggccggctt cgagacggcc ttccggtcct tcgccgccga
     1981 ctgcgtcaag aagaccgact gcccgctggg caccgagtcg cccacccacg ccgccgcccg
     2041 cctcaaggcg ttcttcgccc agctcgacgc cgagcccgtc ccgaccggcg agagccgccg
     2101 actcggtgag gccctggcca ccacgggcgt gatcgccgcc atgtacgacg agtccgcgtg
     2161 gccgcagctg cgcgaggctc tgacccgggc catgggcggc gaggggtccg ccctgctggc
     2221 cctggccgac agctactacg agcgcgagga ggacggcacg tactcgaacc tgatgtacgc
     2281 caacgccgcc gtgaactgcc tcgaccagcc gcccgccttc accggccccg accaggtcgc
     2341 cgccgccgtc ccgtcgttcg agaaggcctc cccggtcttc ggcgcgggcc tggcctgggc
     2401 ctccctgaac tgcgcgtact ggcccaccaa ggccaccggc accgcccacc gcatccacgc
     2461 cgacggcgcc ccgcccatcc tcgtcgtcgg caccacccgc gacccggcga ccccctacaa
     2521 gtgggccgag tccctcgccg cccagctctc ctccggcagg ctcctgacct acgagggcga
     2581 cggccacacg gcgtacgggc gcggcagcga ctgcatcgac accgcgatca acaccttcct
     2641 cctcgacggc acggtccccc cgtccggcaa gcgctgccgc tgagccgccg ctgagccgcc
     2701 accgactcgt gtgcgaggca ccccctggac ctgtgtagac ttgtccccgc tgctgcaccc
     2761 gacaggggat cgcacggcat gccgccttag ctcagttggc cagagcaacg cactcgtaat
     2821 gcgtaggtct cgggttcgaa tcccgaaggc ggctcagctg aagcccagct cagactcagt
     2881 ctgagctggg ctttttcgct cggcggatac gccgacggcg tcgggcggcc gacctcgggc
     2941 gcgggattga cgctgcctgg ggtgccggcc cgctctacag cacgtccgcg gcgtgcagcg
     3001 ccgcggacgc cgggatgaca agggcgcaag cgagccaccg ggagagcagg cggtggccga
     3061 gcagcaggcg gaaaccggcg atggcgacgg ccccccacgt cagtgacatc aacagggagg
     3121 gaacggcgtc ctggatccgt tccacggtcg tccgctcgcc cagcccccac ccggcgaaac
     3181 ttcccgcgac tacggacgcg atcaggaaga aggccagaaa ggccagtccc agcgcccggc
     3241 gaatgatgcg tatgccgacc gtgggctcgg tgtccatgcc atctttcctg tccggctcaa
     3301 tcggcatgct tctcagcggg tgtactggtg gcacagcgcg taatcgaaga ccggcttcag
     3361 gcgagaggtc ccgtcggagc gccggccgtc acggtggaac tggtgattcg gcatcttgtc
     3421 atgggtgccg gcgggcacca gatgtacgcc ggcaccggcc ggtccaaccg cccgtcttcg
     3481 cggcctcggc ccaggtctcg gccctggcgg gcgatcgcga ggacaggcaa gccgtcacat
     3541 cagctggtcc agaagtccca ccagcgggtc aggatcagca tcccgatgat cccgatgtgc
     3601 agcacgggca gcacccaggt gaactcaccg aagaagccgc gcaccgcgac cggggcgggc
     3661 agcaggcgct tgcgtacggt gtgcgaggtc acgtaccaga acatgacgat cgtcgcgacc
     3721 cacgccgaac agcaccacag gcacagcgag ttgatcgtgt agagcgactg gtgcatcagc
     3781 caggtgcaga atccgacccc gaacagcgtc ccggcgttca gcccgagcca gtaccagcgc
     3841 cgcagccgcg cccccgacag cagcgccgcg ccgatcgcga tcaccatgcc gtacgtcacc
     3901 agccccagca tcgggttggg gaacccgaac accgacgcct gctcgctctt catgatgttg
     3961 ccgcaggaga cgaccgggtt caggctgcag cccggagtga agttcgggtc ctccagcagc
     4021 ctgaacttgt cgagcgtgat cacccatgcc gccagcagcc ccaccgcacc ggtggccacc
     4081 agcatccacg cgaacgcccg gcccgcgccg accgtcgtgt cccgctcctc ggcaacctcg
     4141 tcccgggcga ggggcgcctc gccctccacg cgcgtcatcg tcatggcggc gacgctacac
     4201 gccgaatcct ttcgtcgcag ttttccccgt tttcttccac cgaaacgagg gaagggtccc
     4261 ggcggtaggg gtcaacacac catccgccgg ggccccttcg agctgacagt cgtcaggtac
     4321 gcaccctgcg cacgggcagc aggcagtggg cgctcttcgc caccaggtcg tcgtcgccga
     4381 ggaacgccag caactcctcc tcgctgaccg actttccctg cacggcttcc ggccacggaa
     4441 gcagcgcgca gatcaggtag gccgtgccgc tctcgtgggc ggccttctcc cactccggct
     4501 gcaccgggca ctgcaccttc atgaagggca gattgaggac ggcctgttcg gcctccacca
     4561 gcagcgtgat cgggatccgg gtctcgcggg acaggtccgt caccttgtca ccgatcgaca
     4621 ggccggtctc ctccagcttg gaacgcagcg cgtccgcccc ggcctcggga ccgtcacggc
     4681 cgtccccgag ggagtacacc atcaggaacg gcgtaacggg tgagtcggcg tcggactcgg
     4741 gcttgctggc ccaggcgatc acggcgaggg tgcccagaag ggattcctgc cggcccggcg
     4801 tcacggcggt ggaagaagtc atgctgccgg atggtagtgg tgccttccgg cgcgtccggg
     4861 tgcgtttcca cccgttcggc cgatacgcgg gcctctgatt cacctggccg ggtaagggat
     4921 gcggtgacgt ccttgcggaa cggttcgcga gcggggtcgt tgtgcccggt gggagccatg
     4981 acgagcaggg agcccgatcg atgatcgcca gaccgccgct gccccgcctc ctgccgcgcc
     5041 gccgcgccgt tctgcgggcg ctgttcacgc tgaccgtcac cgcgttcacc ggcgggtcgc
     5101 tcgcccatgt cgccaccacg ccaagcgccg gggcggcgcg gcgatccacg ggggagtccg
     5161 acgagatgta ccggggccgg cacatccagg tacgcccggt ggccgggagc cggttcgacg
     5221 tacgggtcga cggccggccc ctgcatctga tgcggtgcgc cgacggcggc tacctcaccc
     5281 tcgtcgacca ctaccagtcg taccccacac ccctcgcggc cgcccgcgcc gccgtggacg
     5341 agctgggctc cgcccaactg gccccaggag cacaccatgg cgtacgtacg taagaaccag
     5401 cgcgacctga cgggcgccga gcggaagaag ttcgtcagcg ccgtgctggc cctgaagcgg
     5461 agcggccggt acgacgactt cgtccgtacg cacatcaggt actacaccgc ggacggcgac
     5521 gacgatctgc gggtcgcgca catggcgccg tccttcctgc cctggcaccg gcagtacctg
     5581 ctggagttcg agcgggccct caggagcgtc gacccgacgg tgaccgtgcc ctactgggac
     5641 tggacccgcg acaacacccc cgccgcgtcc ctgtggggcg aggacttcat gggcggcaac
     5701 ggccggcgcg gcgaccgcca ggtgatgacg gggcccttcg cgtacgccaa ggggaactgg
     5761 cccatcacgg tcggagtgac cgagagcccc ttcctgaccc gtaacttcgg caacccggcc
     5821 aaccccatcg acctgcccac ccggcaggag ctcgcacgcg cgaccgccga gacggtctac
     5881 gactccgccc cgtgggactc caccagccgc accggcttcc gcaacgcgct ggagggctgg
     5941 acccgggaga gcgggaacgg ccgctggcgc aaccacaacc gcgtccaccg gtgggtcggc
     6001 gggctgatgc tcggcggcgc gtccgtcaac gacccggtct tctggctcca ccactccttc
     6061 atcgacctga tctggtcgcg ctggcagagc aagaaccccc gggccggcta tctgcccgcc
     6121 accccgcccc cgccgggctc cgcccagcac cggcgggtcg tcgccctcga cgagcggatg
     6181 ccgccgttcg aggtcacccc cgccgacgtg gccgaccacc accgcgtcta ccgctacgcg
     6241 tgacggccgc ccggacgcgg aacggccccc cggccggtgg gccggggggc cgtacgcgcg
     6301 gtcgggttca gccgccgtag ccgcccttgt ggtccttgtg ctggacgttc gcgcactggt
     6361 tgccgaacgc cgggttgagc aggccgatga tgtcgatggt gttgccgcac aggttgatcg
     6421 ggatgtcgat cgggacctgg accgcgttgc cggagatgac accgggggac tcgacaactg
     6481 cggcgtcggc gtcggcgccg gccacggcca tgccggatcc gctcaggacg acagcgcccg
     6541 tgccgacgat gacggcggca gccttcgcga tgcgagacat caaaatctcc ttgtacgtgg
     6601 aaatccgacc gcggacctgc ggtcgtcact cccctacaac gctgcttttc aggccgggta
     6661 acggccgcgc cactccaaca gccggtcgtg gcgcagggtt cgcccttttg gccgtgtgaa
     6721 gggagtgcgg gaaaggggag tgcgggaaag ggagtggggc gggggcgcgg gtcagttctc
     6781 ctgctgcgcc aggccgagcg tcgagtccag ggctccggtc accgtcggcg cgaggaagcc
     6841 ggcctccggc agcttgaggc ccggcaggcc gagtgactcg gcgtgcggca gggtgatggg
     6901 gaggccgagg atcgcgcccg gcgtggtggc atggatggcc gaggcggggg ccgagatcag
     6961 tgcccggccg gtcggctcgc cctccacgac gttcggcagc ggtgcgctga ccagcgtttc
     7021 cgggacgccc gtcgctacgg gaagttgcgg caggagcacg ggctgggcct ggcccttccc
     7081 gtgcgcggtg agccccatgg gcgctcccgt cagggggatg gggacaccgg tacgcagcgt
     7141 cggcgtctcc gggagtacgg ttccgagacc gtgcaggggc acgtccaccg gtgcgacggg
     7201 tacggtggcg gccgccgccg cgggcgacgc catcgccgcc gatgcgaggc aggtgacgac
     7261 aaaccccatg ctggcctgag atttcaggtt cattgctgcg tacgccttcc cgggcttatg
     7321 agtacggtgc tgcctcaaag aacgagcacg cgccgagaag tgcgtcagaa ttcgcctgat
     7381 tgcagcaatg cggcccgccg ggtgacggcg ggccggcgcc cgggcatgcc gaaaggccga
     7441 cgggaaactt ccttgtttcc cgtcggcccc ggcagcgtca cgtacgacgt catactctcc
     7501 cagcgcactg ctgggagtat tgctgagcgt attgcaggcg tattcgccgg gcgtattact
     7561 tgcccagcgc gggcagcccg cccaggagcg gctgcgcggt gtccttcgcg ggctccagcc
     7621 caccggcggc gtcctgcacc ttgtcgacgg ccttgccgtt ggtcaccttg cccaccgcgt
     7681 cggtgggaac tgcctcggtc agaccgcgct tgcccagggc ttcgactcct ccgttgaggc
     7741 tggagggggt gaggctgtcg acggcgaagg cgggcgcggc cgcgcccaga gccatcacgg
     7801 aaccggcgac gaccgcagca accttggtgg gcttcattgg ggtgagtcct tcctggaatg
     7861 gcgtctgttc gctgcaactc acgcacatca caggccactt gagatcacct gtgcagcgct
     7921 ctgtcttctg taacgagccc tccgcgtggg ggaaactccg cgagccgtga attctcggca
     7981 ctgcacccga atgatacgaa tcgatcctat tctcagatca acgtgcgagg gcgaggacga
     8041 gggaaggccg acggccgtgt cggctcagcc ggggggatgc gggagcgggc tgaggcggca
     8101 cggccgtccg ggggccgggg agaccggggg cgggggccta gaggccggtg gctgggagcg
     8161 tggtggccgg gagggtgacg gccgggaggg ggttcatgcc cgtcgtcggc gtaccggtca
     8221 tcttggcgac gtcgcgcatg atcgtgtcga ccaggttctt ggtggctgcc tggatctggg
     8281 acgccgacgt ggccccttgg ccgttcaccg cggcggcgac catgtcgtcg atcgccttgt
     8341 ccatcgtggc gagggccttg tccaggtccc cgtccatggt ggtgtccgag gtggtgtccg
     8401 tatccacgat gtccatggag atgtcgtcgg ccgtcatacc ggtgccggtg ccggtgccag
     8461 tgccggtggc ggcggtgttc ttttcctcca ccgccttctt cagggcctcg atggcggccg
     8521 tgaggtcctt catctgctgg ctcatgtcgg tggcggggag cgtctgggcg ctgctcgtgc
     8581 ccgagagggt gccgagaacg gagtcgagca cgctgccgat cgagccgagg atgtcactga
     8641 tgtcctcggg cgccttggcc tgctgctcct tggcgggctg gtgggtggag tgggcgatgg
     8701 cgggtcccgc cgtaccgagc gtgagcgcgg cacagagcgc ggtgaccgcc acacggcggg
     8761 gtgtcaggag acgcatgcct tgtccttcca gtgatgcgtc gagaacgtga catcaccgtg
     8821 agtccgctta ttgcggcccg caaacgcatg cgtgtcgacg tgtgaatagg cccgacgggt
     8881 gaatcgggcc gcctgacctg ccgggagaag agaccggcgc tccgtctccg ggcggcgggg
     8941 gcgcgccgcc gatagggtgg cctgcccttg tgtggacaca tgaaaagatg cgccaccccc
     9001 atgggggtgg cgcatctttt cgcgcgcgcc ggctgtgcgc gcggcgtgct gtctcagtcg
     9061 ttgatgcaga cgttcccgaa ggtggggttg agcgcgccga tgatgtcgat ggtgttgccg
     9121 cacaggttga tcgggatgtg gatcggcacc tggatcacgt tgccggtgat gacaccgggg
     9181 gacttcactg ccgcggcctc cgcgtcggcg tcggcgtgcg cgacgccggt cgcaccggcg
     9241 acggaggcgg cgacggcgga ggtcaggacg gctgccttcg cgatacggga catggtgacg
     9301 aactcctagg ttggtgaggc aaagcggccg acgtggccgg ctacaccaac aacgcccgag
     9361 catttcgccg gttgcgcgac cgaatgggtg acacattcgc cggggcgttc gcgggcgaca
     9421 cgccacacca tgaacgagac gtgaaaataa gaaagttgct ccggtcgtga ctcaaatgcc
     9481 actgattgtg tttgttgagt tgtaacgttt gtcccgctgc ttccgctctc ttccagcggg
     9541 aatcatctgt gcgcggtcct cactcgtcaa agcggagaac cagtatgggg aattcgaagg
     9601 ttcttacggt gcggcgcggt ccagctcagc gctgttacgt cgcgccgaac tccgacacca
     9661 cgcatttccc gacgacgagc cggaccggtc ggcatcccgc gcgccgctga cgttcgccgc
     9721 gcgccgcaaa gcgccgaccc ctccctgcca cccgcttcgt agaaggtttc gtagaaaggg
     9781 tcacgcgtgc gtcccccact caccgcattg catgttgtcc agccgggtga cggcggggtg
     9841 gcccgggtgg tcaccgacgt ggtcggcgca caggtggacc tgggaatgcg ggtggccgtc
     9901 gcctgcccgc ccggtacgcc gctggcggtg gcggccggcg cgcggggcgc cgaggtgcac
     9961 ccgtggcggg cgggccgcga gccgggtcca cggctggtgg tcgagagccg aaggatcgcc
    10021 gagctcgtcg agctcgtacg ccccgacctg gtgcacgcgc acagtgcgaa ggccgggctg
    10081 gccgcccgga tcgccgtacg gggccgcgtg cccaccgtct accagccgca cgcctggtcg
    10141 ttcgaggcgg tcgacggggt caccggcgcg ctcgccaggc ggtgggaacg ctgggccgcc
    10201 cgctggaccg accgggtcat ctgcgtcagc gaggcggaac ggcggcgcgg tgagtacgcg
    10261 ggcgtacggg cgtcctggtc cgtcgtccac aacggcgtcg acaccgcccg gttcaccacc
    10321 ggaccggacc gggcggccgc ccgtgccgcg ctcgggctgc ccgcctcggc accgctcgtg
    10381 gtgtgcgtgg ggc
//
