LOCUS       NZ_LIYH01000003        11476 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB03234 scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_LIYH01000003
VERSION     NZ_LIYH01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB03234
  ORGANISM  Streptomyces sp. CB03234
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 941528)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941528)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905885.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:52:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,127
            CDSs (total)                      :: 7,053
            Genes (coding)                    :: 6,894
            CDSs (with protein)               :: 6,894
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 70 of 159
            Pseudo Genes (incomplete)         :: 112 of 159
            Pseudo Genes (internal stop)      :: 10 of 159
            Pseudo Genes (multiple problems)  :: 33 of 159
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 472967
            Orig. end                         :: 484443
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIYH01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11476
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11476
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11476
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6476](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6476
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(399..412)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.06"
                     /tool="antismash"
     gene            446..1291
                     /locus_tag="AMK26_RS15320"
                     /old_locus_tag="AMK26_15115"
     CDS             446..1291
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890194.1"
                     /locus_tag="AMK26_RS15320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15115"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_073755173.1"
                     /transl_table=11
                     /translation="MSAVAALCAVLAGCGSSTGTRPAAPPAQPPAVASLSPARVPARMP
                     PTMAPGPGGLTPVFERGPSAGGDKTVALTFDADMTADQGPRAARGERFDNPALIDTLRR
                     LKVDATVFMTGRWAEQYPGQARAIGDDPRFEIANHSYSHHAFKAPCYGLPALDRHDMAA
                     DVRRAFDAFRTAGVRNVVPYFRFPGGCYDDDALRALASARVTAVQWDVVGGDAFATDAD
                     AVAEQVLAGVRPGSLVVLHCTRSAAPVTEQAIRRIVPELRERGYRFVKVSELMAAARGT
                     "
     gene            1359..2393
                     /locus_tag="AMK26_RS15325"
                     /old_locus_tag="AMK26_15120"
     CDS             1359..2393
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089010.1"
                     /locus_tag="AMK26_RS15325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15120"
                     /product="aldo/keto reductase"
                     /protein_id="WP_073755174.1"
                     /transl_table=11
                     /translation="MRYTLFGRTGLRVSELALGAMTMGHGGDDAMSRRILDAYADAGGN
                     FVDTADSYSAGGSETVLGTLLEGRRDRFVLASKYTCASREGDVNSGGNHRKNLVQSVEE
                     SLTRLRTDRLDVLWVHARDEFTPVEEVMRALNDLVRAGKVLYLGVSDWPAWEIAQANTL
                     AELRGWTCFAGSQLRYNLLERTPERELLPQARGFDQTVMAWAPLASGKLTGKYRRGEPG
                     RLSDGVRENDREAEVITTVLEIAEQGGWSPAQVALAWLRGRPGNIVPLIGASRPEQLAD
                     NLACLGVELDAGAVERLDRVSAVPLGFPHDFLRDPGVVRNVHGDRHADIDDRRTWRASR
                     SVRR"
     gene            complement(2390..2590)
                     /locus_tag="AMK26_RS15330"
                     /old_locus_tag="AMK26_15125"
     CDS             complement(2390..2590)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523443.1"
                     /locus_tag="AMK26_RS15330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15125"
                     /product="hypothetical protein"
                     /protein_id="WP_073755525.1"
                     /transl_table=11
                     /translation="MDDMGDVDERDDSYCETPAVTEAPGGPPYAACVLCQEPTEYPEST
                     KGITLCPVCEWREAERTACSG"
     gene            complement(2646..3323)
                     /locus_tag="AMK26_RS15335"
                     /old_locus_tag="AMK26_15130"
     CDS             complement(2646..3323)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153329.1"
                     /locus_tag="AMK26_RS15335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15130"
                     /product="AIM24 family protein"
                     /protein_id="WP_073755175.1"
                     /transl_table=11
                     /translation="MKSDLFASENMAQQATVPGMTLQNAKSVKYAVNGEMHARQGSMIA
                     YRGNLQFERKGQGLGGMLKRAVTGEGLPLMAVRGQGEAWFAHEAQNCFIVDIEQGDALT
                     VNGRNVLCFDATLSYDIKTVKGAGMTGGGLFNSVFTGYGKLAVICDGNPVVIPVTTHQP
                     VYVDTDAVVGWSAQLNTSLHRSQSLGSMIRGGSGEAVQLMLQGEGFVIVRPSELTPEKA
                     KQS"
     gene            3394..4062
                     /locus_tag="AMK26_RS15340"
                     /old_locus_tag="AMK26_15135"
     CDS             3394..4062
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563201.1"
                     /locus_tag="AMK26_RS15340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15135"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_241837906.1"
                     /transl_table=11
                     /translation="MSSNDAPQFVLPLVVRMERAEPPARTDALETAARAVLVMLSDDRS
                     LGDGEWAEAMRAWQDARIRKVVRRARGAEWRKACLLPGITVTGDAAEVRVFPPVPLDGW
                     PKELAKLQVSGTELDDTAEPPPPAPGQAVLWLNPDLRMSTGKEMAQVGHGAQLLWWAMP
                     DADRKAWREAGFPLAVRTADPARWAELTASGLPLVRDAGFTEIAPGSCTVVSEFPPRDD
                     "
     gene            4129..4893
                     /locus_tag="AMK26_RS15345"
                     /old_locus_tag="AMK26_15140"
     CDS             4129..4893
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890188.1"
                     /locus_tag="AMK26_RS15345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15140"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_179166825.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVVTVGALAFPVWSYADRSGTGQANLNASTVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSQAIKDAGDHLVVGHTDLDQRAR
                     DVAAKLGVELPNQPTEQQQGWLRELSAASGEEYERKFANLLRNAHGKVFALVAQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGKVDFDAIADEAAGNATASPSGPPAPNGNLPPA
                     PSPAVPTGTDQSFTSRPSTQPGPPTAINTTRP"
     gene            5001..6476
                     /locus_tag="AMK26_RS15350"
                     /old_locus_tag="AMK26_15145"
     CDS             5001..6476
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965512.1"
                     /locus_tag="AMK26_RS15350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15145"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_073755178.1"
                     /sec_met_domain="DUF692 (E-value: 4.5e-97, bitscore: 320.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKPELGIGIGWRPEIAEAVEALPGVDWVEVVAENICAGPGGGHL
                     PDSLLRLRERGVTVVPHGVSLGLGGADRPDEGRLAELAAKASALGAPLVTEHIAFVRAG
                     GPLTASPALEAGHLLPVPRTWEALDVLCENVRIAQDALPVPLALENIAALFAWPGEELT
                     EGQFLAELVERTGVRLLIDVANLHTNHVNRGEDPAKALAELPVEAIAYVHVAGGVERDG
                     VWHDSHAHPVPRPVLDILADLASRVSPPGVLLERDDDFPPAEELAAELASIRATLRRAG
                     AEAPAPPGAREPSPAAGTGAETSAAPMTGTGAATSARPVAGAGPVTGAGAATGTGPPVT
                     GARPAVRAGAAAGGARQRVGITQAALLSALVAGTPVPEGFDHARLRVQSRALAGKRAGV
                     VAKVAPELPEILGGGYRAAFLAYAASRPMRGGYRRDALDFAEHLLIAGRPEDPAARRRL
                     TLWWRDRAGARPPGRAARLVRAARAALVGRRAA"
     gene            6473..7456
                     /locus_tag="AMK26_RS15355"
                     /old_locus_tag="AMK26_15150"
     CDS             6473..7456
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612179.1"
                     /locus_tag="AMK26_RS15355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15150"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_241837907.1"
                     /transl_table=11
                     /translation="MNVLALLLYVAVAVSSALLINGVAASRRGPGGSLHHRVEAAFLNG
                     GPARVVDSALAALAADGRVAVGGPGIVHVVRPVGHDPVERAVLHELAAAPSGALHLVRL
                     TAMRNPAVQEIGDRLAARGLIVPRRAGRTWRRWATIQAGACFLLIFGSIAATVAEYATA
                     EFGDMPFPFVIKVLPALLAGLVIGMVCGGVSSRRVTGAGRRALAAYRRAHAHLIEPGDL
                     VALHGLRALPDPVLQGQLLAAARMYDRRRDGGGTQPDELFATTTVTVWCASADTGGSSC
                     GGGGGGGGGGGGSACSGGSSCGSGGGGGGGGGGGSSCGGGGGGGGD"
     gene            7561..8136
                     /locus_tag="AMK26_RS15360"
                     /old_locus_tag="AMK26_15155"
     CDS             7561..8136
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /locus_tag="AMK26_RS15360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15155"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_073755179.1"
                     /transl_table=11
                     /translation="MRPLTGTVLVVVALTATIAALVFPVWSYADRAGTPPARLAAGSVP
                     TRWGPLSAADRDFVVRVRLTGLWALPAAQQATGRAPSRAVEAAGRHLATDHTYLGVRVR
                     DVAARLRLALPSRPTAEQRTWLDRLTEAQGRAYEELFATVLRRDHGTLFSVAAEIRATT
                     RNTLVRALADDAVRLLLADLNALETSVS"
     gene            8181..8939
                     /locus_tag="AMK26_RS15365"
                     /old_locus_tag="AMK26_15160"
     CDS             8181..8939
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972877.1"
                     /locus_tag="AMK26_RS15365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15160"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073755180.1"
                     /transl_table=11
                     /translation="MFWVPFLLVTWAAAAVSCARLCLAAVEAAQPVPPAAQDGDRELNL
                     YEAAFLAGGPQRVTELTLVSMHRRRRLWLAHTGWATLVDPDPEGADDLERSVIGAIGPE
                     GQSRIAAVRAAASAADAVRALADRLVAAGLAVPDAARSGIVAALRAVRRSAALTVALAA
                     ATVLLLPAGHAPDARAMAWFALPLVLTLGCLVIARIEVHPYTRWASPAGQSLLTKVRVG
                     PCDPLAAVAIGGVRALDDPALRAALAPGQR"
     gene            complement(9115..9828)
                     /gene="hemQ"
                     /locus_tag="AMK26_RS15370"
                     /old_locus_tag="AMK26_15165"
     CDS             complement(9115..9828)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888541.1"
                     /locus_tag="AMK26_RS15370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15165"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_073755181.1"
                     /transl_table=11
                     /translation="MSAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRTGYADE
                     VQELFDQLAAKDITVRGTYDVSGLRADADLMIWWHAETSDALQEAYNLFRRTKLGRALE
                     PVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGFPDVRANTVASFSLGDYEWLLAFEADELYRIVDLMRHLRGSEARMHVREEVPFY
                     TGRRKSVADLVAGLA"
     gene            complement(9833..11281)
                     /gene="hemG"
                     /locus_tag="AMK26_RS15375"
                     /old_locus_tag="AMK26_15170"
     CDS             complement(9833..11281)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965516.1"
                     /locus_tag="AMK26_RS15375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_15170"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_073755182.1"
                     /sec_met_domain="DAO (E-value: 9.6e-08, bitscore: 27.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNADTGDTPESGHVVVIGGGIAGLAAAHRLITAGRRVTLLEATTR
                     LGGKLHAGEIAGAPVDLGAESMLARRPEAIVLARAVGLGDRLQPPATTSAAVWTRDALR
                     PMPKGHVMGVPGDAAAVAGLLTAEGLARIEHERDLPPADPAALGDDVAVGRYVADRLGR
                     EVVDRLVEPLLGGVYAGDAYAISMRAAVPKLFEAARAHGTLTEAVRSLQQGAQQQTGPA
                     FMGIAGGIGTLPGAVADAITAQGGEVLTDTPVHALTRAARGGWDIRTGDRVLHADAVIV
                     ATPAWSASELLAAESPAAAAELAAVEYASMALVTLAFRRSDLAGVLPAGSGFLVPPVDG
                     RTIKASTFSSQKWKWTDEGSGDLVVLRTSVGRYGEEEHLHREDADLVSVSLKDLADATG
                     LAARPVATEVTRWIGGLPQYPVGHLARVARIRDAVAKLPRLRVCGAVYDGVGIPATIAS
                     AWRAADEILATPTLAHGTLSDERE"
     misc_feature    11387..11407
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
ORIGIN
        1 ggccagcgag ccgcgcttgg gcttgaagtg gggggcgagc cgggtgacct ggcggtcgac
       61 gaccggcgcg cgggcgaagc gcgggtcgtt ggtgaccatc cggacgtcgt cgtaccgggt
      121 gaccagccac gcccagccct cgccgtgggg cagctggacg cggctgaccg gtccttcgcg
      181 catcagctcg gcgagcaccg ggtcgaactc cgtcccggtg atgtccagcg caggccagtg
      241 ccgtacgggc ggcggaccca ggatgggccg tgtcgcggtg tcctcgatca tgggtccgtc
      301 cgtcccttcg agcggtgggg ttccccctca cgatcacccg ggcggggccg gacatcgcgc
      361 tggactactc cggacggaag cggcatggac gtcccccatt gcgatgatca tatattttcc
      421 gcctgtgact acttttgtac ggaatgtgtc ggccgtggct gccttgtgtg ccgtcctcgc
      481 cggctgcggc agctccaccg gcacccgccc cgccgcgccg cccgcgcagc cgcccgccgt
      541 ggcgagcctc tcaccggccc gggtgcccgc ccggatgccg ccgaccatgg cgcccggccc
      601 cggcgggctc acgcccgtgt tcgaacgggg cccctcagcc gggggtgaca agaccgtggc
      661 gctcaccttc gacgccgaca tgacggccga ccagggcccc agggccgcgc gcggcgagcg
      721 gttcgacaac ccggccctga tcgacaccct ccggcgcctg aaggtcgacg ccaccgtctt
      781 catgaccgga cgctgggcgg agcagtaccc cggccaggcg cgggccatcg gcgacgaccc
      841 gcgcttcgag atcgccaacc actcctacag ccaccacgcc ttcaaggccc cctgctacgg
      901 gctgcccgcc ctcgaccggc acgacatggc ggccgacgta cgccgggcct tcgacgcgtt
      961 ccgcacggcc ggggtgcgca acgtcgtgcc ctacttccgc ttccccggcg gctgttacga
     1021 cgacgacgcg ctgcgcgcgc tggcctcggc ccgggtgacg gccgtgcagt gggacgtggt
     1081 cggcggcgac gccttcgcca ccgacgccga cgcggtcgcc gaacaggtcc tggcgggcgt
     1141 acggccgggc tcgctggtgg tgctgcactg cacccgcagc gccgcccccg tcaccgagca
     1201 ggccatccgc cgcatcgtcc cggagctgcg cgagcgcggc taccgcttcg tcaaggtctc
     1261 ggagctgatg gccgccgcac gcggcacgta aacgcagttg cgccgcggtg cgctccaccc
     1321 cctaccgtca cgggtacgac ctgacgaggg gacacaccgt gcgttacaca ctgttcggcc
     1381 ggaccggtct gcgcgtgagc gagctggcgc tgggggccat gaccatggga cacggcggcg
     1441 acgacgccat gagccgccgc atcctcgacg cgtacgcgga cgcgggcggc aacttcgtcg
     1501 acacggccga cagctactcc gccggcggct ccgagaccgt cctcggcacg ctgctggagg
     1561 gccgccggga ccggttcgtc ctggcctcca agtacacctg cgccagccgc gagggcgatg
     1621 tgaactccgg cggcaaccac cgcaagaacc tcgtgcagtc ggtggaggag agcctcaccc
     1681 ggctgcgcac cgaccgcctg gacgtgctgt gggtgcacgc ccgggacgag ttcaccccgg
     1741 tcgaggaggt catgcgcgcc ctgaacgatc tcgtacgcgc cggaaaggtg ctctacctcg
     1801 gcgtgtccga ctggcccgcc tgggagatcg cccaggcgaa caccctcgcg gagctgcgcg
     1861 gctggacctg cttcgccggt tcgcagctgc gctacaacct gctggagcgc acgcccgagc
     1921 gggagttgct gccccaggcc cgcggcttcg accagaccgt catggcgtgg gccccgctgg
     1981 cctccggcaa gctcaccggc aagtaccgcc gtggtgagcc gggccggctg tccgacggcg
     2041 tacgggagaa cgaccgggag gccgaggtga tcaccaccgt cctggagatc gccgagcagg
     2101 gcggctggag cccggcccag gtggcgctgg cctggctgcg cggccgcccc ggcaacatcg
     2161 tccccctcat cggtgccagc cgccccgagc agctcgccga caacctcgcg tgcctgggcg
     2221 tggagctgga cgccggggcc gtcgagcggc tcgaccgggt gagcgcggtg ccgctcggct
     2281 tcccgcacga cttcctgcgg gaccccggcg tcgtgcgcaa cgtccacggc gaccggcacg
     2341 cggacatcga cgaccggcgc acctggcgcg cctcccggtc agtacgtcgc tagccggagc
     2401 aggcggtgcg ctccgcctcc cgccactcgc agaccgggca cagcgtgatg cccttcgtcg
     2461 actccgggta ctcggtcggc tcctggcaca ggacacacgc tgcgtacggc ggcccgcccg
     2521 gggcctccgt cacggccggc gtctcgcagt acgaatcgtc cctctcgtcc acgtcgccca
     2581 tgtcgtccat gtcgtccatg tatacgagcg tacgcactcc cgcacggccg gtgccgctcc
     2641 acggctcagc tctgcttcgc cttctccggg gtgagctcgc tgggccgtac gatcacgaag
     2701 ccctcgccct ggagcatcag ctgcacggcc tcgccggagc cgccgcggat catcgagccg
     2761 agggactgcg agcggtgcag cgaggtgttc agctgggcgc tccagccgac gacggcgtcg
     2821 gtgtcgacgt acaccggctg gtgggtggtg accgggatga ccacggggtt gccgtcgcag
     2881 atcacggcga gcttgccgta cccggtgaag acgctgttga agaggccgcc accggtcatc
     2941 ccggcgccct tcacggtctt gatgtcgtac gagagggtcg cgtcgaagca cagcacgttg
     3001 cggccgttga cggtgagcgc gtcgccctgc tcgatgtcga cgatgaagca gttctgggcc
     3061 tcgtgcgcga accacgcctc gccctgaccg cggacggcca tcagcggcag gccctcgccg
     3121 gtgacggcgc gcttgagcat gccgcccagg ccctgcccct tgcgctcgaa ctggaggttg
     3181 ccgcggtagg cgatcatcga tccctgccgg gcgtgcatct caccgttgac ggcgtacttg
     3241 accgacttcg cgttctggag ggtcatgccg ggcactgtcg cctgctgcgc catgttctcg
     3301 gacgcgaaaa gatcgctttt catgcgggca tcgtgtcccg gaacgacccg ttccgccaag
     3361 aacgggaccg tgaccggctg gcaaactgtc ccgatgagca gcaacgacgc accccagttc
     3421 gtcctccccc tggtcgtacg gatggaacgg gcggagcccc cggcccgtac cgacgccctg
     3481 gagaccgccg cgcgcgcggt gctcgtgatg ctgtccgacg accggtcgct cggcgacggc
     3541 gagtgggccg aggcgatgcg cgcctggcag gacgcgcgga tccgcaaggt ggtgcgccgg
     3601 gcgcgcggcg cggagtggcg caaggcctgc ctgctgccgg gcatcacggt cacgggcgat
     3661 gccgcggagg tacgggtctt cccgcccgtc cccctcgacg gctggcccaa ggagctggcc
     3721 aagctccagg tctcgggcac ggaactggac gacaccgccg aaccgccgcc gcccgcgccg
     3781 ggtcaggcgg tgctgtggct caacccggac ctgcgcatgt cgaccggcaa ggagatggcc
     3841 caggtgggcc acggcgccca gctgctgtgg tgggcgatgc cggacgcgga ccgcaaggcc
     3901 tggcgggaag ccgggttccc gctcgcggtc cgtaccgccg acccggcccg gtgggccgaa
     3961 ctgaccgcct cggggctgcc gttggtgcgt gacgcgggct tcacggagat cgctccgggg
     4021 tcgtgcacgg tggtctccga gtttccgccg cgggacgact gaacgctccc gcgcgtgtat
     4081 acgtacctcc cagtaatgcc atcaggttcg ggaggcacca ccttgttgtt gcgacgcatc
     4141 aacggcacgg cactcatcat cgcggccctc gtcgtcacgg tcggcgcgct ggcgtttccg
     4201 gtctggtcgt acgccgaccg ctccggaacg gggcaggcca acctcaacgc gtccaccgtg
     4261 gcgacgcagt ggggaccgct gtcggcgacg gaccgtgact tcctggtgaa ggtgcggctg
     4321 gcggggctgt gggagctgcc ggccgggcag caggcgatcg agcgggcgcc gagccaggcc
     4381 atcaaggacg cgggcgacca cctcgtcgtc ggccacaccg acctcgacca gcgggcgcgt
     4441 gacgtggcgg cgaagctggg cgtggagctg ccgaaccagc cgacggagca gcaacagggc
     4501 tggctgcggg agctgtcggc ggcgagcggc gaggagtacg agcggaagtt cgccaacctg
     4561 ctccgcaacg cgcacggcaa ggtcttcgcg ctggtcgcgc aggtgcggca caccacgcgc
     4621 aacacgctga tacggcagct ggcgagcgac gccaaccaga cggtgctgga ccacatcacg
     4681 atgctggagg cgaccggcaa ggtcgacttc gacgcgatcg ccgacgaggc ggcggggaac
     4741 gcgacggcga gcccgtccgg tccgccggcg cccaacggca acctgccgcc ggcgccgtca
     4801 ccggccgtgc cgacggggac ggaccagtcg ttcacgtccc ggccctcgac gcagccgggt
     4861 ccgccgacgg ccatcaacac gacccgcccg taacctcacc cgtaacctca aatgtagctc
     4921 tgaacagcac ctcttctgga ttggccgcgc tccggcgggg ccatcacccc tccgaccgac
     4981 gcaggaggag ggggacggac atggtgaagc cggaactggg catcgggatc ggttggcgcc
     5041 cggagatcgc ggaagcggtg gaggcgctgc ccggggtgga ctgggtggag gtggtcgcgg
     5101 agaacatctg cgccggcccg ggtgggggcc acctccccga ctcgctgctg cggctgcgcg
     5161 agcggggcgt caccgtcgtc ccgcacggcg tgtcgctggg gctgggcggc gcggaccggc
     5221 ccgacgaggg gcggctcgcg gaactggcgg cgaaggcctc ggcgctgggg gcgccgctgg
     5281 tcacggagca catcgccttc gtacgggccg gggggccgct caccgcgtcg cccgcgctgg
     5341 aggcgggcca cctgcttccg gtgccgcgta cgtgggaggc gctggacgtg ctgtgcgaaa
     5401 acgtccgtat cgcgcaggac gcgctgccgg tgccgctggc gctggagaac atagcggcgc
     5461 tgttcgcctg gccgggcgag gaactgacgg aggggcagtt cctggccgag ctggtggagc
     5521 gtaccggcgt ccgcctcctg atcgacgtgg cgaacctgca caccaaccac gtcaaccggg
     5581 gcgaggaccc ggcgaaggcg ctggcggagc tgccggtcga ggcgatcgcg tacgtccacg
     5641 tcgcaggcgg cgtcgaacgg gacggtgtct ggcacgacag ccacgcacac ccggtcccgc
     5701 gcccggtcct cgacatcctg gcggacctcg cctcccgggt cagcccgccg ggcgtcctgc
     5761 tggagcgcga cgacgacttc cccccggcgg aggagctggc cgccgaactg gcctcgatcc
     5821 gcgcgacgct gcggcgggcg ggcgccgagg ccccggcgcc ccccggcgcc cgcgaaccgt
     5881 cgccggcggc aggcaccggg gccgagacaa gcgcggcgcc catgaccggc acgggggcgg
     5941 ccacgagcgc aaggccggtc gcgggcgcag ggccggtcac gggcgccggg gccgccacag
     6001 gcacggggcc gccggtcacg ggcgcgaggc cggcggtacg ggcgggtgcg gcggccgggg
     6061 gtgcccggca gcgggtgggg atcacgcagg ccgcgctgct gtccgcgctg gtggccggga
     6121 cgcccgtgcc cgaggggttc gatcacgcgc ggctacgcgt acagagccgg gcgctggccg
     6181 gcaagcgggc cggggtggtg gccaaggtcg cgcccgagct gccggagatc ctcggcggcg
     6241 gttaccgtgc cgcgttcctc gcgtacgccg catcccgccc catgcgcggc ggctaccggc
     6301 gcgacgcgct cgacttcgcg gagcacctgc tgatcgccgg gcggccggag gatcccgccg
     6361 cccgccggag gctcaccctg tggtggcggg accgcgccgg agcccgcccg cccggccggg
     6421 ccgcccggct ggtgcgcgcc gcccgcgccg cgctggtggg gaggcgcgcc gcgtgaacgt
     6481 cctcgccctc cttctgtacg tcgccgtcgc cgtctcgtcg gccctgctca tcaacggggt
     6541 ggccgcctcc cgtcgcggcc ccggcggctc cctccaccac cgagtggagg ccgcgttcct
     6601 gaacggcggg cccgcccggg tcgtcgactc cgcgctcgcc gcactcgccg cggacggccg
     6661 cgtggccgtc ggcgggccgg gcatcgtgca cgtggtacgc ccggtcggcc acgaccccgt
     6721 cgagcgcgcc gtgctgcacg agctcgccgc cgcgcccagc ggcgcgctgc acctcgtacg
     6781 gctcaccgcc atgcgcaacc ccgccgtgca ggagatcggc gaccggctcg ccgcccgcgg
     6841 gctgatcgtg ccgcgccgcg cggggcgcac ctggcgccgg tgggccacca tccaggcagg
     6901 tgcctgcttc ctgctcatct tcggctccat cgccgcgacc gtcgccgagt acgccaccgc
     6961 cgagttcggt gacatgccgt tccccttcgt catcaaggtg ctgcctgccc tgctggccgg
     7021 cctggtcatc gggatggtct gcggcggcgt cagcagccgc cgggtcaccg gcgcgggccg
     7081 ccgggccctc gccgcgtacc gcagggccca cgcccacctg atcgagccgg gggacctggt
     7141 ggcgctgcac ggactgcgcg cgctgcccga cccggtgctg cagggccaac tgctcgcggc
     7201 ggcgcggatg tacgaccgga gacgcgacgg aggaggcact cagccggacg agctgttcgc
     7261 gaccacgacg gtcaccgtgt ggtgcgcgag cgccgacacg ggcggttcga gctgcggggg
     7321 cggcggtgga ggcggcgggg gcggcggagg ctcggcctgc agcggcgggt cgagctgcgg
     7381 aagcggcggc ggagggggcg gaggcggagg cggcggctcc agctgcggcg gcggaggggg
     7441 cggaggcggc gactgatggc gcccgtaacc caccaacgta ccaactcggc aagggagttg
     7501 ggggagggtc cggcaaccgt cccttcgagc gccgcgccgc ccgctagcgt gcggcgcatc
     7561 atgcgaccgc tcaccggcac cgtcctggtc gtcgtcgccc tgacggcgac catcgccgcc
     7621 ctcgtcttcc ccgtctggtc ctacgccgac cgcgccggca caccccccgc ccgcctggcg
     7681 gccggttccg tacccacccg gtggggcccg ctgtccgccg ccgaccgcga cttcgtcgtc
     7741 cgcgtccggc tgaccggcct ctgggcgctg cctgccgccc agcaggccac cggccgcgcg
     7801 cccagccgcg ccgtcgaggc cgccgggcgg cacctggcga ccgaccacac ctacctcggc
     7861 gtccgcgtcc gtgacgtcgc cgcccggctc cggctggcgc tgccgagccg gcccaccgcc
     7921 gagcagcgga cctggctcga ccggttgacg gaggcgcagg gacgcgcgta cgaggagctg
     7981 ttcgccaccg tcctgcgccg ggaccacggc accctcttct ccgtcgccgc cgagatccgg
     8041 gccaccaccc gcaacaccct cgtccgggcg ctcgccgacg acgccgtacg actcctcctg
     8101 gccgacctca acgccctgga aacgtccgta tcgtgaaaac cctgtggcgc cgcacccgcc
     8161 gcttcgcata gaactcgggc atgttctggg tgcccttcct gctggtgacc tgggccgcgg
     8221 cggccgtcag ctgtgcgcgg ctgtgcctgg ccgccgtcga ggccgcccag ccggttcccc
     8281 ctgccgcaca ggacggtgac cgcgaactga acctgtacga ggccgcgttc ctggccggcg
     8341 gcccgcagcg ggtcaccgag ctgaccctgg tgtcgatgca ccggcggcga cggctgtggc
     8401 tcgcccacac cggctgggcg acgctcgtcg acccggatcc ggagggggcg gacgacctgg
     8461 agcgttccgt gatcggcgcg atcgggcccg aggggcagtc gcggatagcg gcggtacgcg
     8521 ccgcggcgtc ggccgccgac gcggtacggg cgctcgccga ccggctcgtc gcggcgggcc
     8581 tcgccgtgcc cgacgccgcc cggtccggca tcgtcgccgc actgcgggcc gtgcgccgct
     8641 ccgccgccct gaccgtggcg ctcgcggcgg ccaccgtgct gctgctgccc gccggacacg
     8701 cgccggacgc acgtgccatg gcctggttcg cgctcccgct cgtcctcacg ctcggctgcc
     8761 tcgtcatcgc gcgcatcgag gtccacccgt acacgcggtg ggcctccccc gccgggcaga
     8821 gcctgctgac gaaggtgagg gtgggcccgt gcgacccgct cgccgccgtc gccatcggcg
     8881 gggtccgcgc cctggacgac ccggcgctgc gcgcggcgct cgcaccgggg cagcggtagg
     8941 gacgcctgaa aggacgcggg tgcggcgcgc gttcacgcgc gccgcacccg gagccggacc
     9001 cggtgtcgct ggagcggcgg gcgcgcgggc ccgccgtctg atcttccggg tcggtgggtg
     9061 acgcgcgggg gcggttcccc ccgcaccgtc accaggtcgg tcaggtcagg gccctcaggc
     9121 gagacccgcg accagatccg cgaccgactt gcgccggccg gtgtagaacg ggacctcctc
     9181 gcggacgtgc atccgcgcct cggagccgcg caggtgacgc atcaggtcca cgatccggta
     9241 cagctcgtcc gcctcgaagg cgaggagcca ctcgtagtcg cccagcgaga acgaggcgac
     9301 cgtgttggcg cgcacgtccg ggaagccgcg ggccatcttg ccgtggtcgg cgagcatccg
     9361 ccgccggtcc tcgtccggca gcaggtacca gtcgtaggag cgcacgaacg gatacaccga
     9421 cacgtagtcg cgcggcgtct cgtcggcgag gaaggccggg atgtgggacc ggttgaactc
     9481 ggcggggcgg tgcagcgcca tgttggacca caccggctcc agcgcgcggc ccagcttggt
     9541 gcggcggaag aggttgtacg cctcctggag cgcgtccgag gtctcggcgt gccaccagat
     9601 catcaggtcg gcgtcggcgc gcagacccga cacgtcgtac gtgccacgga cggtgatgtc
     9661 cttggcggcg agctggtcga acagctcctg gacctcgtcg gcgtagccgg tccggtcctc
     9721 gggcaggacg tcgcgcagct tgaagacgga ccacagggtg tagcggatga cctcgttgag
     9781 gtccttcgcc ttcttaccgg cgttcgggat cttttcgggc gcactcatag ggctattctc
     9841 gctcgtcact cagggtgccg tgcgccaggg tcggcgtggc gaggatctcg tccgccgcgc
     9901 gccacgcgct cgcgatggtc gcggggatgc cgacaccgtc atacaccgcc ccgcagacgc
     9961 gcaggcgggg gagcttggcg acggcgtcgc ggatgcgggc gacccgggcg aggtggccca
    10021 cggggtactg cggcaggccg cctatccacc gggtgacctc tgtggcgacg ggccgggcgg
    10081 ccagcccggt ggcgtcggcg aggtccttca gggatacgga gacgaggtcg gcgtcctcgc
    10141 ggtgcaggtg ctcctcctcg ccgtaccggc cgacggaggt gcgcagcacg acgaggtccc
    10201 cggatccctc gtcggtccac ttccacttct gggaggagaa cgtggacgcc ttgatggtgc
    10261 ggccgtcgac gggcgggacg aggaacccgg agccggcggg cagaacgcct gccaggtcgg
    10321 agcgccggaa ggcgagggtg accagggcca tcgacgcgta ctccacggcg gccagctcgg
    10381 cggcggcggc cggggactcg gcggcgagca gctcggaggc ggaccaggcg ggggtcgcga
    10441 cgatcaccgc gtcggcgtgc aggacccggt cgccggtacg gatgtcccag cctccccggg
    10501 ccgctcgggt cagggcgtgg acgggcgtgt cggtgaggac ctcgccgccc tgggcggtga
    10561 tggcgtcggc gacggcgccg ggaagcgtgc cgatgccgcc cgcgatgccc atgaaggcgg
    10621 gcccggtctg ctgctgggcg ccctgctgga gggagcgcac cgcctcggtg agcgtgccgt
    10681 gggcgcgggc ggcctcgaag agcttgggca cggcggcgcg catcgagatg gcgtacgcgt
    10741 cgcccgcgta caccccgccg aggagcggtt cgacgagccg gtcgacgacc tctcggccga
    10801 ggcggtcggc gacataccgg ccgacggcga cgtcgtcccc gagggcggcg gggtcggcgg
    10861 ggggcaggtc ccgctcgtgc tcgatacggg cgaggccctc ggcggtcagg aggcccgcga
    10921 cggcggccgc gtcgccggga acgcccatga cgtgcccctt gggcatcggc cggagcgcgt
    10981 cacgggtcca gacggcggcg ctggtggtgg cgggcggctg gagccggtcg ccgaggccga
    11041 cggcgcgggc gaggacgatc gcctcggggc ggcgggcgag catcgactcg gcgccgaggt
    11101 cgacgggggc gccggcgatc tccccggcgt ggagcttgcc gccgagccgg gtggtggcct
    11161 ccagcaaggt cacgcgccgc ccggcggtga tcagccggtg ggcggcggcg aggcccgcga
    11221 taccgccgcc gatgacgacg acatggccgc tctcgggtgt gtccccagtg tccgcgttca
    11281 tgccccctac tctctcaaac gcctctgaca caccgttccg agtcctgacc gtgaccggtt
    11341 cgtgagccgc tcaccggcaa ccggtcccct cccccacacg tccaacaggc acaccattcg
    11401 ccatgtggac cggggggatc atgcgcgcac gtcgtcacag attcgcggcc gtacttctgc
    11461 tcaccgcctc gctcgc
//
