LOCUS       NZ_LIYH01000001        10399 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB03234 scaffold1, whole genome shotgun sequence.
ACCESSION   NZ_LIYH01000001
VERSION     NZ_LIYH01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB03234
  ORGANISM  Streptomyces sp. CB03234
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1475216)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475216)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905885.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:52:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,127
            CDSs (total)                      :: 7,053
            Genes (coding)                    :: 6,894
            CDSs (with protein)               :: 6,894
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 70 of 159
            Pseudo Genes (incomplete)         :: 112 of 159
            Pseudo Genes (internal stop)      :: 10 of 159
            Pseudo Genes (multiple problems)  :: 33 of 159
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1386890
            Orig. end                         :: 1397289
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIYH01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="7"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(186..203)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.83"
                     /tool="antismash"
     misc_feature    223..240
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.03"
                     /tool="antismash"
     gene            331..2352
                     /locus_tag="AMK26_RS06265"
                     /old_locus_tag="AMK26_06175"
     CDS             331..2352
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982763.1"
                     /locus_tag="AMK26_RS06265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_06175"
                     /product="beta-galactosidase"
                     /protein_id="WP_073752738.1"
                     /transl_table=11
                     /translation="MPETTPRGLRRLAYGGDYNPEQWPESIWQEDVRLMREAGVTMVSV
                     GIFSWAGLEPEPGAYDFGWLDRLLDLLHEHGIRADLGTPTVVPPAWFYRAHPDALPVTR
                     EGTRYAFGSRGAICHSSPAYRAAAATITEQLARRYGAHPALALWHVHNEYGVPVSACYC
                     DTCADHFRRWLDARYGSIDAVNDAWGTAFWGQHYTAYEQIDPPRATPTVGNPAQALDYR
                     RFADATMRENFSMERDILHRLSPGVPVTTNFMTALSQCDSVDYWAWGREVDLVTNDHYL
                     ITDGRRTHVNLAMAADLTRSVAGGAPWLLLEHSTSGVNWQPRNPAKRPGEMARNSLAHI
                     ARGSEGAMFFQWRQSRRGAEKFHSAMVPHAGTDSRVWREVVQLGADLGALAELRGTRTV
                     ADAAMVWDWHSWWAQNLEWRPSRDHDARERADSFYETLYDRHLTVDFAHPEADLSAHPL
                     VVVPALYLTTEAAGRNLRAYVENGGTLVVSYFSGIVDEHDAVHPGAYPGALRDVLGLTV
                     EEWSPLGEGETVRITEAAGDGALTGDVWTEFVVPRGAETIWTYADGPAAGHPAVTRHRL
                     GKGTAWYVSTRLAPPALDAVIGAACEDAGIAPRTDLPRDVEVVRRTGDSGTYLFALNHT
                     AHDAKVPLDAPGTELLSGEPVAGHLAVPAGAVCVVRLA"
     gene            2391..3941
                     /locus_tag="AMK26_RS06270"
                     /old_locus_tag="AMK26_06180"
     CDS             2391..3941
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576697.1"
                     /locus_tag="AMK26_RS06270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_06180"
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="WP_073752740.1"
                     /transl_table=11
                     /translation="MYGTTRAATAAVIAAAGLLLAGLPATTADAAATLTNPGFESGATG
                     WSTYSATGQNSASFTEAGGASGSTRLSHWASGAYKVETYQYLSGLTDGTYTLSARVRSS
                     GGQNAAYIALRNCGSPEQRTDLPPTANGAWIRIVTSVKVTGGQCTVSFNSDAHAGQWAN
                     FDDVTFTPGTTGLAIKGGDLSTLPKNEAYGASYRYANGTSGDAMAILKAAGMNYVRLKV
                     WVNPADGYNNKAKVLAMAKRAKAQGMKVLVDFHYSDTWADPGKQYKPAAWSSHTYEQLR
                     KDVYDHTYDVLGALKAQGTTADMVQIGNELNAGMLWEEGRTSNWSQLAGLVTSGANAAK
                     AVSSSTQVALHLAKGGDNAGTRWWFDQAVAYKVPFDVIALSYYGYWHGPLSGLQANLDD
                     TAARYGKPVLVAETAYAHTLANDDFLENNVATADTLAPGYPATPAGQAANLRDVMNVVE
                     AVPNGRGLGVVYWEPAWTAVTGSGWDPADPASGNAWENQAVFDYDGRLLPAANWFRHR"
     gene            complement(4137..4997)
                     /gene="thpD"
                     /locus_tag="AMK26_RS06275"
                     /old_locus_tag="AMK26_06185"
     CDS             complement(4137..4997)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187943.1"
                     /locus_tag="AMK26_RS06275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_06185"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_073752742.1"
                     /sec_met_domain="PhyH (E-value: 2.3e-37, bitscore: 125.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQDLYPTRGASEVVTPRQDPVVWGPVSSPELASFERDGFLAVDQL
                     ITDDEVAVYRAELDRLVTDPAVRADERSIIEPKSQDVRSVFEVHRISEVFARLVRDERV
                     VGRAREILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPRMRTVSVSIALTE
                     NLDTNGGLMIMPGSHTSFLGCAGETPKDNYKKSLQMQDAGTPSDEALTKFADAHGIRLF
                     TGRAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENTAVEPFAAPVRRPEFIGARD
                     FTPVR"
     gene            complement(5001..5399)
                     /locus_tag="AMK26_RS06280"
                     /old_locus_tag="AMK26_06190"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="AMK26_RS06280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_06190"
                     /product="ectoine synthase"
                     /protein_id="WP_073752744.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.6e-54, bitscore:
                     179.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTERHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTATSMWYANHIEAVLCVEGEAELTNDETGETHWIEPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            complement(5432..6700)
                     /gene="ectB"
                     /locus_tag="AMK26_RS06285"
                     /old_locus_tag="AMK26_06195"
     CDS             complement(5432..6700)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 8.5e-72.
                     Score: 233.9. Matches aSDomain:
                     nrpspksdomains_AMK26_RS06285_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="AMK26_RS06285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_06195"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_073752746.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.6e-77, bitscore:
                     256.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALLDYLERDGITHGLDMATTAKRAFLESFQNVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALRALCDRQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSISGYGLPMS
                     LCLFMPELDVWGPGEHNGTFRGNNPAFVTATAALDTYWADGQMEKQTIARGEQVEQALL
                     AICDENSAYGAHFRGRGLVWGMEFTDKSRAAAVCARAFELGLLVETSGPQSEVVKLLPP
                     LTVTPDELDEGLRTLARAVRETA"
     aSDomain        complement(5606..6616)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK26_RS06285_Aminotran_3.1"
                     /evalue="8.50E-72"
                     /label="AMK26_RS06285_Aminotran_3.1"
                     /locus_tag="AMK26_RS06285"
                     /protein_end="365"
                     /protein_start="28"
                     /score="233.9"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHTYLDFFAGAGSLNYGHNNPVLKRALLDYLE
                     RDGITHGLDMATTAKRAFLESFQNVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALRALCDRQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTLSKSISGYGLPMSLCLFMPELDVWGPGEHNGTFRGNNPAFV
                     TATAALDTYWADGQMEKQTIARGEQVEQALLAICDENSAYGAHFRGRGLVWGMEFT"
     gene            complement(6765..7310)
                     /gene="ectA"
                     /locus_tag="AMK26_RS06290"
                     /old_locus_tag="AMK26_06200"
     CDS             complement(6765..7310)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969939.1"
                     /locus_tag="AMK26_RS06290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_06200"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_198943995.1"
                     /transl_table=11
                     /translation="MTAAQADLARARNEFRRPATPAIGIDTPRVEDGAAIWRIARDSEV
                     LDLNSSYSYLLWCRDFAATSLVARGADGEPIAFVTGYIRPQRPHTLVVWQVAVDHDHRG
                     QGLAGVLLDALTARVAGQGVHTVETTITPDNTASDRLFTAYAERRGASLEREVLFNGGL
                     FPEGTHEPEVLYRISPVA"
     misc_feature    complement(7330..7344)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.63"
                     /tool="antismash"
     misc_feature    complement(7466..7481)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7476..7491
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7476..7493)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.57"
                     /tool="antismash"
     misc_feature    7562..7578
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.65"
                     /tool="antismash"
     gene            7785..8897
                     /locus_tag="AMK26_RS06295"
                     /old_locus_tag="AMK26_06205"
     CDS             7785..8897
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /locus_tag="AMK26_RS06295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_06205"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_241837797.1"
                     /transl_table=11
                     /translation="MTSTVIINGDGWGPGVELRQLEYFVAVAEEFGFGRAAERLHTVPA
                     SVSRQIARLEHELGLRLFDRSDRHGVRHGVRLTAAGERLLPEARAALAAAARVRDTAAG
                     ITAGTEGLVRLGTTRAFADRVYRALDTLAARRPGLRVRLERAPQEARLAAVRSGTFDAA
                     LVRTLRRADGVALHPLWSEPLVAALPASHALALGLAEGEAPSLDRLARLPLRLAPRAAD
                     PALHDLVTAALPEWTPGPPFTTLQDTLTELAAHPEPSWALFHPVGPLPPMPRITFRTLP
                     DLTVPVSLAIPSGRPPTPPVQALLEALATTTTPPPPAGTPPKGSAPTGTVPTGTAPTGT
                     VPTGTVPTGTAPTGTATGKAPAGAVTSAVR"
     misc_feature    7800..7814
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.79"
                     /tool="antismash"
     gene            complement(9019..10122)
                     /locus_tag="AMK26_RS06300"
                     /old_locus_tag="AMK26_06210"
     CDS             complement(9019..10122)
                     /NRPS_PKS="Domain: Aminotran_5 (52-293). E-value: 9.3e-30.
                     Score: 95.7. Matches aSDomain:
                     nrpspksdomains_AMK26_RS06300_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337954.1"
                     /locus_tag="AMK26_RS06300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK26_06210"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_073752750.1"
                     /sec_met_domain="Aminotran_5 (E-value: 8.1e-24, bitscore:
                     80.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSHPLLDLPPLTADRFAAIERRVAALLATEQDVVITQGEALLPLE
                     GCIRAGARPGSTALNVITGPYGQTFGNWLRDCGATVVDLEVPFHTAVTADRIGEALERN
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFHLDAVASVGAEPVLPDAWGVDLCV
                     ISAQKAMGGPAGVSAVSVSTRAWERFAANPAAPRRSYLSLLDWKERWIDAGRTALPHAP
                     AQLEMLALEACLERIEAAGLDTLMARHASAAAATRAGALALGGGLEPYVYEAKDAAPVA
                     TTLRAPAGVDAAELVAAALAADPSLPLAAGGGALAKEMVRVNHYGPDADRAVVEASLTA
                     LGAALTELTGSAADVPAARRAVTATWT"
     aSDomain        complement(9244..9966)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK26_RS06300_Aminotran_5.1"
                     /evalue="9.30E-30"
                     /label="AMK26_RS06300_Aminotran_5.1"
                     /locus_tag="AMK26_RS06300"
                     /protein_end="293"
                     /protein_start="52"
                     /score="95.7"
                     /tool="antismash"
                     /translation="RPGSTALNVITGPYGQTFGNWLRDCGATVVDLEVPFHTAVTADRI
                     GEALERNPEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFHLDAVASVGAEPVLPDA
                     WGVDLCVISAQKAMGGPAGVSAVSVSTRAWERFAANPAAPRRSYLSLLDWKERWIDAGR
                     TALPHAPAQLEMLALEACLERIEAAGLDTLMARHASAAAATRAGALALGGGLEPYVYEA
                     KDAAPVATTLRAPAGVDAA"
ORIGIN
        1 gcggcgcccc cgccgtcgtc cgaggacgac ccgcacccgg tgagggcggt cgcgccgagg
       61 gcgacggcgg tggcgatggc gatggcggaa acgcggcggt gcagcgcgtg cggcatgggg
      121 gcttctccct gaccgagggg ggcggcctcc gccgtagtgg gaggcctgcc atgcttctgt
      181 gagcgttcac agtagagaaa catcccagac acttgtcaat ggttgttgct gtgcggttat
      241 gttgcgttcc gacggcacgc cgagtgtgtg cacgttccca cttgttcgga acgcggtggg
      301 aagctttccc gaggaaccag gagaaacccc atgccggaga ccactcccag aggcctgcgc
      361 cggctcgcct acggcgggga ctacaacccc gaacagtggc cggaaagcat ctggcaggag
      421 gacgtccggc tgatgcgcga ggccggcgtc accatggtca gcgtcggcat cttctcctgg
      481 gccgggctcg aacccgagcc cggcgcctac gacttcggct ggctcgaccg gctcctggac
      541 ctgctccacg agcacggcat ccgcgccgac ctcggcacgc ccaccgtcgt cccgcccgcc
      601 tggttctacc gcgcccaccc cgacgcgctg cccgtcaccc gcgagggcac gcggtacgcc
      661 ttcggctcgc gcggcgccat ctgccacagc tcaccggcgt accgcgccgc cgccgcgacc
      721 atcacggaac agctcgcccg ccgctacggc gcccaccccg ccctcgccct gtggcacgtc
      781 cacaacgagt acggcgtccc ggtcagcgcc tgctactgcg acacctgcgc cgaccacttc
      841 cgccgctggc tggacgcccg ctacggctcg atcgacgccg tcaacgacgc ctggggcacc
      901 gccttctggg gccagcacta caccgcgtac gagcagatcg acccgccccg cgccacgccc
      961 accgtcggca accccgccca agcgctcgac taccgccgct tcgccgacgc caccatgcgc
     1021 gagaacttct cgatggagcg ggacatcctg caccggctgt cccccggcgt ccccgtcacc
     1081 accaacttca tgaccgcgct cagccagtgc gacagcgtcg actactgggc ctggggccgt
     1141 gaggtcgacc tggtcaccaa cgaccactac ctgatcaccg acggccgccg tacgcacgtc
     1201 aacctcgcca tggccgccga cctcacccgc tccgtcgccg gcggcgcccc ctggctgctg
     1261 ctggagcact ccaccagcgg cgtcaactgg cagccgcgca accccgccaa gcgccccggc
     1321 gagatggccc gcaactccct cgcccacatc gcacgcggct ccgagggcgc catgttcttc
     1381 cagtggcgcc agtcccggcg cggcgccgag aagttccact ccgcgatggt gccgcacgcc
     1441 ggaaccgact cccgcgtctg gcgcgaggtc gtccagctcg gcgccgacct cggtgccctg
     1501 gccgagctgc gcggtacgcg gacggtcgcg gacgccgcca tggtgtggga ctggcactcc
     1561 tggtgggcgc agaacctgga gtggcgcccc agccgggacc acgacgcccg cgagcgcgcc
     1621 gacagcttct acgagaccct ctacgaccgg cacctcaccg tcgacttcgc ccaccccgag
     1681 gccgacctgt ccgcccaccc cctggtcgtc gtccccgctc tgtacctcac caccgaggcc
     1741 gcgggccgca atctgcgcgc ctacgtcgag aacggcggca ccctcgtcgt ctcctacttc
     1801 tccggcatcg tcgacgagca cgacgccgtc caccccggtg cgtacccggg cgccctgcgg
     1861 gacgtcctcg gcctcacggt ggaggagtgg tcgccgctgg gcgagggcga gacggtacgg
     1921 atcacggagg cggccgggga cggggcgctg accggcgacg tgtggaccga gttcgtcgtc
     1981 ccgcgcggcg ccgagaccat ctggacgtac gccgacggcc cggccgccgg ccaccccgcc
     2041 gtcacccgcc accgcctggg caagggcacc gcctggtacg tctccacccg cctcgccccg
     2101 cccgccctgg acgcggtcat cggcgcggcc tgcgaggacg cgggcatcgc gccacgcacc
     2161 gacctgcccc gcgacgtcga ggtggtgcgc cgcaccggcg acagcggcac ctacctgttc
     2221 gccctcaacc acaccgcgca cgacgccaag gtgccgctgg acgcccccgg caccgaactc
     2281 ctcagcggcg agcccgtcgc cggccacctc gccgtcccgg cgggcgcggt ctgcgtcgtc
     2341 cggctcgcgt aacccccgca acgccacaca catcgtcgaa gggacgcgac atgtacggca
     2401 ccaccagagc ggccacggcc gccgtcatcg ccgccgcggg cctgctcctc gccgggctgc
     2461 ccgcgaccac ggccgacgcc gcggccacgc tcaccaaccc cggcttcgaa agcggcgcca
     2521 ccggctggtc cacctactcc gccaccggcc agaacagcgc ctcgttcacc gaggccggcg
     2581 gggcgagcgg cagcacccgc ctctcccact gggcttccgg cgcctacaag gtggagacct
     2641 accagtacct gtccggtctc acggacggta cgtacacact gagcgcccgc gtccgctcca
     2701 gcggcgggca gaacgccgcg tacatcgcgc tgcgcaactg cggcagcccc gagcagcgca
     2761 ccgacctgcc gcccaccgcg aacggcgcct ggatacggat cgtgacctcc gtcaaggtca
     2821 ccggcggcca gtgcacggtg agcttcaact ccgacgcgca cgcgggccag tgggccaact
     2881 tcgacgacgt caccttcacg cccgggacga ccggcctcgc catcaagggc ggcgacctgt
     2941 cgacgctccc gaagaacgag gcgtacgggg cgtcctaccg gtacgcgaac ggcacgagcg
     3001 gcgacgcgat ggccatcctc aaggcggccg gcatgaacta cgtacgcctc aaggtgtggg
     3061 tgaaccccgc cgacggctac aacaacaagg cgaaggtcct cgccatggcc aagcgcgcca
     3121 aggcccaggg catgaaggtc ctggtggact tccactactc cgacacctgg gccgaccccg
     3181 gcaagcagta caagcccgcg gcctggtcct cgcacacgta cgagcagctg aggaaggacg
     3241 tgtacgacca cacgtacgac gtcctcggcg ccctcaaggc gcagggcacc acggccgaca
     3301 tggtccagat cggcaacgag ctgaacgccg ggatgctgtg ggaggagggc cgcacgtcga
     3361 actggtcgca gctggccggg ctggtcacct ccggcgcgaa cgccgccaag gccgtctcgt
     3421 cctccacgca ggtcgccctg cacctggcga agggcggcga caacgcgggc acccgctggt
     3481 ggttcgacca ggccgtcgcg tacaaggtgc ccttcgacgt catcgccctg tcgtactacg
     3541 gctactggca cggcccgctc tcgggcctcc aggccaacct ggacgacacc gccgcccggt
     3601 acggcaagcc cgtgctggtc gcggagacgg cgtacgcgca cacgctcgcc aacgacgact
     3661 tcctggagaa caacgtcgcc accgccgaca ctctggcccc cggctacccg gcgaccccgg
     3721 cgggccaggc cgccaacctg cgggacgtga tgaacgtcgt cgaggccgtg ccgaacggcc
     3781 ggggcctcgg cgtcgtctac tgggagcccg cctggaccgc ggtgaccggc agcggctggg
     3841 acccggccga cccggcgtcg ggcaacgcct gggagaacca ggccgtgttc gactacgacg
     3901 gcaggctgct gccggccgcg aactggttcc gccaccgctg acggcacgcc gccgagggcg
     3961 ccgcgttgac ggcacccctg cggacagcac cgcgccgacg cacccgcgag gggcgggacc
     4021 gcaccccggt cccgcccctc cgcgtgtgtg ccggacgcgt caccgcaccg gcgtgaagtc
     4081 ccgcgcgccg atgctgcgtc tcccggggga cgacccccgg acccccggca gcggggtcac
     4141 cgcaccggcg tgaagtcccg cgcgccgatg aactccggtc gccggaccgg ggccgcgaac
     4201 ggctccaccg ccgtgttctc cacgctgttg aacacgatga agacgttgct gcgcgggtac
     4261 ggcgtgatgt tgtcgcccga gccgtgcatc gcgttgcagt cgaaccaggt cgccgagccg
     4321 gccctgccgg tgaacagccg gatgccgtgc gcgtcggcga acttggtcag cgcctcgtcc
     4381 gagggcgtgc ccgcgtcctg catctgcagc gacttcttgt agttgtcctt cggcgtctcg
     4441 cccgcgcagc ccaggaacga cgtgtgcgac cccggcatga tcatcaggcc gccgttggtg
     4501 tcgaggttct cggtcagcgc gatcgacacg gacaccgtcc gcatccgcgg cagcccgtcc
     4561 tccgcgtgcc acgtctcgaa gtccgagtgc cagtagaagc cggaggcgcc gaagcccggc
     4621 ttcacgttga tccgcgactg gtggacgtac acgtcggagc cgaggatctc gcgggcccgg
     4681 cccaccacgc gctcgtcgcg caccagccgg gcgaacacct cgctgatccg gtggacctcg
     4741 aagaccgacc gtacgtcctg ggacttcggc tcgatgatcg agcgctcgtc ggcgcggacc
     4801 gccgggtcgg tgaccagccg gtcgagctcc gcccggtaga cggcgacctc gtcgtccgtg
     4861 atcagctggt cgacggcgag gaagccgtca cgctcgaacg acgccagctc cggcgacgac
     4921 accggccccc agacgaccgg gtcctggcgc ggggtgacga cctcggacgc gcctcgggtg
     4981 gggtagagat cctgcattgt tcagccctcc tcggtcagca gcgggtacac accgttctcg
     5041 tcgtggtcct cccgtccggt gacaggaggg ttgaacacgc acacgcaccg gaagtcggtc
     5101 ttcgggcgca gggtgtggcg ctcatggccg tcgagcaggt acatcgtgcc cggctcgatc
     5161 cagtgcgtct cgcccgtctc gtcgttggtc agctcggcct cgccctcgac gcacagcacg
     5221 gcctcgatgt ggttggcgta ccacatggag gtcgcggtcc ccgcgtacag cacggtctcg
     5281 tgcagggaga agccgacctt ctccttggcg agcacgatgc gcttggactc ccaggtgcca
     5341 gaggccgcct tcacatggcg ctcggtgttc tcgatgtcct tgaacgatcg gacgatcacg
     5401 gtggtgcaac ttcctttcgt acgggaaaga ttcaggcggt ttcgcggacc gcgcgggcca
     5461 gcgtccgcag cccctcgtcc agctcgtcgg gcgtgaccgt cagcggcggc agcagcttga
     5521 cgacctcgct ctgcgggccc gacgtctcca ccagcagccc cagctcgaag gcgcgtgcgc
     5581 agaccgccgc ggcacgcgac ttgtcggtga actccatgcc ccacaccaga ccgcggcccc
     5641 ggaagtgggc gccgtaggcg gagttctcgt cgcagatcgc cagcagcgcc tgctcgacct
     5701 gctcgccgcg cgcgatggtc tgcttctcca tctgaccgtc ggcccagtag gtgtccagcg
     5761 cggcggtggc cgtgacgaag gccgggttgt tgccgcggaa ggtgccgttg tgctcaccgg
     5821 ggccccacac gtccagctcc ggcatgaaca ggcagagcga catcggcagg ccgtagccgc
     5881 tgatggactt cgacagggtg acgatgtccg gggtgatgcc cgcctcctcg aaggagaaga
     5941 aggcacccgt gcggccgcag cccatctgga tgtcgtcgac gatcagcagc atgtcctggc
     6001 ggtcgcacag cgcccgcagc gcgcgcagcc actcggcgcg ggcgacgttg atgccgccct
     6061 cgccctgcac ggtctccacg atcacggcgg ccggcttgtt caggccggag ccctggtcct
     6121 ccaggagccg ctcgaaccac aggaagtccg ggacctggcc gtcgaggtag ttgtcgaacg
     6181 gcatcggggt gccgtgcacc agcgggatac cggccccggc gcgcttgaac gcgttgccgg
     6241 tgacggcgag cgagccgagc gacatgccgt ggaaggcgtt ggtgaacgac acgatcgact
     6301 cgcgcccctt caccttccgg gccagcttca gcgcggcctc cacggcgttg gtgccggtcg
     6361 ggcccgggaa catcaccttg tagggcaggt cgcgcggccg caggatcacg ttctggaagg
     6421 actccaggaa cgcgcgtttc gcggtcgtcg ccatgtcgag gccgtgggtg atgccgtcgc
     6481 gctccaggta gtcgagcagg gcgcgtttca gtacggggtt gttgtggccg tagttgagcg
     6541 agccggcgcc ggcgaagaag tcgaggtagg tgtgtccgtc ctcgtcggtg aggcgtgcgc
     6601 cctgcgcacg gtcgaacacg gcgggccaac cgcggcagta gctgcgcacc tccgactcaa
     6661 gggtctcgaa gacactcagg gcgggcgggg tgatggtcac agcggttcgt ctcctcggtg
     6721 cgggtgtctc aggcgtgtcg ggcggcgttc ggtcggtggc gcgttcaggc gacggggctg
     6781 atgcggtaga gcacctcggg ctcgtgcgtc ccctcgggga acagcccccc gttgaagagg
     6841 acctcacgct ccagtgacgc gccacggcgc tccgcgtacg ccgtgaagag ccggtccgac
     6901 gccgtgttgt ccggcgtgat cgtggtctcc acggtgtgga cgccctgtcc ggccacgcgc
     6961 gccgtcagcg cgtcgagcag cacgccggcc agcccctggc cacggtggtc gtggtccacg
     7021 gcgacctgcc agacgacgag ggtgtgcggg cgctggggcc tgatgtaccc cgtcacgaag
     7081 gcgatcggtt ctccgtcggc gccgcgcgcc accagtgagg tggcggcgaa gtcgcggcac
     7141 cacagcaaat agctgtacga ggagttgagg tccaggacct cggagtcgcg ggcaatgcgc
     7201 cagatcgcgg ctccgtcctc cactcgcggg gtgtcgatac cgatggccgg cgtggccggt
     7261 cggcggaatt cgtttcgggc acgtgcaagg tctgcttgtg cggcggtcat gcggattgaa
     7321 cttacccagc aatttctgaa attgcatcgg ggtgaggggt tgggtgagcg cctgacctgt
     7381 gttatcgcgc gggcgcgaga tcaacgggcg accgctcgcc gaacgccgat atttgcccgg
     7441 atatgtacgc gcaaaacggg cgtgatgtgg agtcggtcac actggagtaa ccggcttgac
     7501 acctgcccga attgcccgcc gaatccttat gaaatctttg cgtttagggt ccgggatacg
     7561 gggcagaaga atggggcaag ctgcccggga aggtgccctc cctaaagcag tgccaggaat
     7621 tcggtgaagt attcccggtc cattccgtgt tcgccatttc ggcgatgctc ccgctcggcc
     7681 gccgcccccg ccggttgagt cgttccggcc tcttgccccg tacctgtgcc tacatgtgcg
     7741 taggcgccac ggcggtacgg gaagaagacg ggacagggaa gcggttgacc agcacagtga
     7801 tcatcaacgg cgatgggtgg gggccaggag tggagctgcg tcagctggag tacttcgtcg
     7861 cggtcgccga ggagttcggc ttcggacggg ccgcggagcg gttgcacacc gtccccgcat
     7921 cggtgagccg gcagatcgcg cggctggagc acgagctggg gctgcggctg ttcgaccgct
     7981 cggaccggca cggcgtacgg cacggggtgc gcctcacggc ggccggcgag cggctgctgc
     8041 ccgaggcccg tgccgcgctg gccgcggccg cccgggtgcg ggacaccgcc gccgggatca
     8101 ccgcggggac ggagggcctg gtacggctcg gcaccacccg ggccttcgcc gaccgcgtct
     8161 accgcgccct cgacaccctg gccgcgcgcc gcccggggct gcgcgtacgg ctggagcggg
     8221 cgccccagga ggcccgcctc gcggccgtac gctccggaac gttcgacgcc gccctcgtac
     8281 gcacgctgcg gcgggccgac ggcgtggccc tgcacccgct gtggtccgag ccgctggtcg
     8341 ccgcgctgcc cgcctcgcac gcgctcgcgc tcggcctcgc ggagggcgag gccccgagcc
     8401 tcgaccggct ggcgcggctg cccctgcggc tggcgccgcg cgccgccgac ccggccctgc
     8461 acgacctggt cacggccgcg ctgccggagt ggacgcccgg gccgccgttc accacgctcc
     8521 aggacacgct caccgagctg gccgcccacc cggagccgtc gtgggcgctg ttccacccgg
     8581 tgggcccgct gccgccgatg ccccggatca ccttccgtac gctgccggac ctcacggtcc
     8641 cggtctccct ggccatcccc tcgggccgcc cacccacccc accggtccaa gccctcctgg
     8701 aagccctggc aaccaccacc acgcccccac cccctgccgg gacgcccccc aagggctcgg
     8761 cgcccacggg tacggtgccc acgggtacgg cgcccacggg gacggtgccc acgggtacgg
     8821 tgcccacggg tacggcgccc accggtacgg ccacggggaa ggcgccggcc ggggcggtca
     8881 cgtctgcggt ccgttgagcg ccggggcggg cgcccgcgcg cggcggcgcc caccgtaacg
     8941 gcagccgccg gggcggtcac gtccacggtg cggtgaccgc acggacggtg cccgtcgggg
     9001 cggtgaccgg tgccgccgtc aggtccatgt cgcggtgacc gcccggcggg cggccggcac
     9061 gtccgccgcc gagccggtca gctcggtcaa ggcggcgccg agggccgtca gcgaggcctc
     9121 caccacggcc cggtcggcgt ccggcccgta gtggttgacc cggaccatct ccttggccag
     9181 cgccccgccg cccgccgcca gggggagcga cgggtcggcg gccagggcgg cggccaccag
     9241 ctcggcggcg tccaccccgg ccggggcgcg cagcgtcgtc gcgaccgggg cggcgtcctt
     9301 cgcctcgtac acgtacggct ccaggccgcc acccagcgcg agcgcacccg cgcgggtggc
     9361 cgccgcggcc gacgcgtggc gggccatcag cgtgtccagg cccgccgcct cgatgcgctc
     9421 caggcacgcc tccagcgcca gcatctccag ctgcgcgggc gcgtgcggca gcgccgtgcg
     9481 gccggcgtcg atccagcgct ccttccagtc cagcagggag aggtacgagc ggcgcggcgc
     9541 ggccgggttg gccgcgaacc gctcccacgc gcgcgtgctg accgacaccg ccgagacccc
     9601 cgccgggccg cccatcgcct tctgcgcgct gatgacgcac aggtccacgc cccaggcgtc
     9661 cggcagcacc ggctccgcgc cgaccgaggc caccgcgtcc aggtggaaca gcgcgccgtg
     9721 cgcccgtacc acctcaccga tctcggcgac cgggttggtg ttgccggtcg ccgcctccgc
     9781 gtgcaccagc gacacgaagt cgatctccgg gttccgctcc agcgcctcgc cgatccggtc
     9841 ggccgtcacc gcggtgtgga acggcacctc caggtccacc accgtcgcgc cgcagtcgcg
     9901 cagccagttg ccgaaggtct gcccgtacgg cccggtgatc acgttcaggg cggtcgaacc
     9961 gggccgcgcg cccgcgcgga tgcagccctc cagcggcagc agcgcctcgc cctgcgtgat
    10021 caccacgtcc tgctcggtgg cgagcagcgc ggccacgcgc cgctcgatgg ccgcgaagcg
    10081 gtcggcggtc agcgggggca gatcgaggag ggggtgcgac acggggcggc tctcttctcg
    10141 cttcacgtac ggactgcttc gccgagggta cttccgccct cgtagagtgc tggacatgag
    10201 cgtggtgctg catgtgacgg ggcgggtact ggcgggcccg gacgacgtac gcgacgagct
    10261 gtgggtggtc gacgggcggg tcacctacga ccggccgtcc gccgcgcggg acgtcaggac
    10321 ggtgacgggc tgggcgctgc ccggactggt cgacgcgcac tgccatgtcg ggctcgacgc
    10381 ccacggggcc gtcgacgag
//
