LOCUS       NZ_LIWA01000009        10504 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB01883 scaffold9, whole genome shotgun sequence.
ACCESSION   NZ_LIWA01000009
VERSION     NZ_LIWA01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB01883
  ORGANISM  Streptomyces sp. CB01883
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 344396)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344396)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905855.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:34:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,089
            CDSs (total)                      :: 8,009
            Genes (coding)                    :: 7,788
            CDSs (with protein)               :: 7,788
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 85 of 221
            Pseudo Genes (incomplete)         :: 169 of 221
            Pseudo Genes (internal stop)      :: 25 of 221
            Pseudo Genes (multiple problems)  :: 50 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:33:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 332395
            Orig. end                         :: 342899
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIWA01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10504
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10504
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10504
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5504](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5504
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(322..337)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.71"
                     /tool="antismash"
     misc_feature    391..410
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.37"
                     /tool="antismash"
     misc_feature    686..702
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.07"
                     /tool="antismash"
     misc_feature    complement(687..701)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.19"
                     /tool="antismash"
     misc_feature    734..749
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.9"
                     /tool="antismash"
     misc_feature    complement(961..975)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 21.66"
                     /tool="antismash"
     gene            998..1237
                     /locus_tag="AMK32_RS36105"
                     /old_locus_tag="AMK32_35360"
     CDS             998..1237
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="AMK32_RS36105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35360"
                     /product="chaplin"
                     /protein_id="WP_073900609.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSAAVAAVAGGAGVAAADSGAHGAAANSPGVLSGN
                     AVQVPVHVPINVCGNTVDIIALLNPAFGNACYSD"
     gene            1409..2338
                     /locus_tag="AMK32_RS42085"
                     /old_locus_tag="AMK32_35365"
     CDS             1409..2338
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMK32_RS42085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AMK32_35365"
                     /product="hypothetical protein"
                     /protein_id="WP_073900611.1"
                     /transl_table=11
                     /translation="MRALPPRRLASTVLCAAVLVGITGPAVVAADSSREHARAASPAFA
                     PAAEKLLAQVRALDHTGTVPRPVIDLLEQSLEKGKLPAAEARRLGDAAKKALAEAAAHP
                     ASAVAPSATATPTAPAATPTAPAASTAPTTPAEPAAATPAASTPAASTPAAAASTPAAS
                     TPASASAPASASASASASPSQSVAPDVAASPSQSVAAKNRDDAKPAARDAVDDLLAALD
                     KAIDNLVDAITAQLDQVLASADGLVDGLLELIDSVLGGVPSTLPSPLSAAAAVPDVAVP
                     SLAPAEAVPSLPALPATSTSAAPELPAG"
     misc_feature    2441..2456
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.23"
                     /tool="antismash"
     gene            2533..2817
                     /locus_tag="AMK32_RS36115"
                     /old_locus_tag="AMK32_35370"
     CDS             2533..2817
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672804.1"
                     /locus_tag="AMK32_RS36115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35370"
                     /product="hypothetical protein"
                     /protein_id="WP_073900613.1"
                     /transl_table=11
                     /translation="MKSLKAAVVVAGSVALAGAAAPAFASTAHDLPPMSLNGGLASIAE
                     HPPTVQDLMPLQHQSDALDTENKDSVLSTVKDATTTLNKPGLLGGLPLS"
     gene            2978..3481
                     /locus_tag="AMK32_RS36120"
                     /old_locus_tag="AMK32_35375"
     CDS             2978..3481
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672803.1"
                     /locus_tag="AMK32_RS36120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35375"
                     /product="DUF5949 family protein"
                     /protein_id="WP_073900615.1"
                     /transl_table=11
                     /translation="MTSATSATRPFRAADLGTLVLLAWMARNEEGDGRTPCLLACSLGD
                     GPDGPEAASAAVERLLGDVGLTVGGEPVDGTTRPGLKVSMLVVAGQIVLQLPNVSSQVT
                     PPAEWVQVVEKLGYTYFMFTTRICPSTGPRRPVQGQELAVFAGSPETTDAAAHVFLPAR
                     GLRT"
     gene            3481..4065
                     /locus_tag="AMK32_RS36125"
                     /old_locus_tag="AMK32_35380"
     CDS             3481..4065
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136066.1"
                     /locus_tag="AMK32_RS36125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35380"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_073900618.1"
                     /transl_table=11
                     /translation="MQRHAGGGRAFALLIVLTGAAGLLASWVITLDEFKLLENPAFVPG
                     CSLNPVVSCGSVMKSDQASVFGFPNPMLGLVAYGIVVCVGVSLLAGARFPRWYWLLFEA
                     GCLFGIGFVTWLQFESLYRIDALCLWCCLAWIATILLFWYVTSLCVRHGLLPAPAWLRT
                     FFAEFTWVLPVLHIGVIGMLVLTRWWDFWTS"
     misc_feature    complement(4077..4093)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 21.87"
                     /tool="antismash"
     gene            4167..4682
                     /locus_tag="AMK32_RS36130"
                     /old_locus_tag="AMK32_35385"
     CDS             4167..4682
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="AMK32_RS36130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35385"
                     /product="hypothetical protein"
                     /protein_id="WP_073900620.1"
                     /transl_table=11
                     /translation="MKSTTRGTLAAVVTGIAATVGAAGPAAASGAVPVSVPLGGVSTAL
                     DMEVPHLGAQVPLVKPGAPEGPRYVTGRLLPERTLPQLPVSSGLPGVDARSPLPHVLGD
                     GGFDHVGLDAPASDLRALAPGLSVDAPLTAPDAGNFGLPRPKLPQAGILTPVLQNVSDA
                     NLQSGPGL"
     gene            5001..5504
                     /locus_tag="AMK32_RS36135"
                     /old_locus_tag="AMK32_35390"
     CDS             5001..5504
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="AMK32_RS36135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35390"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_073900621.1"
                     /sec_met_domain="melC (E-value: 9.4e-47, bitscore: 153.0,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVGAGGVAVGTEQQQVSGGAGRPSRREVTRGLIASAAALALAPV
                     VAASRPVPVNEEPDGDSFDETYRGRRIQGFLVPTGGRKATDVEWQITIDGRPLHLMRRA
                     DGSWLSMVDHYTSYATPLEATRAAVDQIGPRQRLRDLAPGPVGGEQPGGHMHMAMGGRH
                     GVRA"
     gene            5488..6354
                     /locus_tag="AMK32_RS36140"
                     /old_locus_tag="AMK32_35395"
     CDS             5488..6354
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="AMK32_RS36140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35395"
                     /product="tyrosinase family protein"
                     /protein_id="WP_073900623.1"
                     /transl_table=11
                     /translation="MAYVRKNVRNLTATERRRFVDALLALKRRGEYDEFVRMHIQYYTA
                     DGEDGLRTAHMAPSFLPWHRRFVLDLERALRRIDSSVTVPYWDWTRDRSTTSVPWTKDL
                     LGGNGRRSDRQVMTGPFAYASGHWTIKEGVTDGRFLARDLGRSASPIQLPTAKELQGAL
                     DDPVYDAWPWNSTVRKGFRNKLEGWGSGSGSASWHNHNRVHRWVGGAMLGGASVNDPVF
                     WLHHAFLDLQWYRWQRAHRSHRYLPAEPPGARDDQHGRVVARHQKLPPWNDTPDQLEDM
                     SGVYRYA"
     gene            complement(6466..6765)
                     /locus_tag="AMK32_RS36145"
                     /old_locus_tag="AMK32_35400"
     CDS             complement(6466..6765)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672799.1"
                     /locus_tag="AMK32_RS36145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35400"
                     /product="chaplin"
                     /protein_id="WP_073900624.1"
                     /transl_table=11
                     /translation="MSRIAKSAVVALGTGAVVVGGAGLALADAGAHGLAAGSPGVLSGN
                     AVQVPVHVPVNLCGNTVDIVGLLNPAFGNSCLNHSGGGSIPGHPGPSHDGGYGN"
     gene            6972..7523
                     /locus_tag="AMK32_RS36150"
                     /old_locus_tag="AMK32_35405"
     CDS             6972..7523
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001133.1"
                     /locus_tag="AMK32_RS36150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35405"
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_073900626.1"
                     /transl_table=11
                     /translation="MDTDNPSEPAERSRQLIGLLAEERRLRAFAAVALGADSAGRVAEA
                     AGLTAKDTALALLRLREQGAVTDRPDGGLTVSYGLLRELARTERSARPSGDAVLDTFVR
                     GGRLVRLPAQWTRKKQVLRHIAEQSFEPGVEYPERVVNERLRSWCEDADDTDHVTLRRY
                     LVDLGHLRRSDGVYKRVTAA"
     gene            complement(7531..8490)
                     /locus_tag="AMK32_RS36155"
                     /old_locus_tag="AMK32_35410"
     CDS             complement(7531..8490)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672797.1"
                     /locus_tag="AMK32_RS36155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35410"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_073900628.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 2.7e-55, bitscore:
                     180.7, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCVVLDDFQGVATGMADWSVIEDRVEVVALREHLDGEDALAE
                     ALAGFDIVVTLRERVAFPGSLIARLPRLKLLVASGMRNSVIDYDAARAHGVTVCGTASS
                     GTPPVELTWALLLGLARGVVEESTALRSGGPWQSTVGADLHGSRLGLLGLGRIGSRVAR
                     VGLAFGMRVGAWSQNLTEERAKEEGVELAASKEELLAESDFVSVHLALGDRTRGLVGAA
                     ELAVMKPTAYLINTSRAAIVDQEALLAALHEGRIAGAGVDVFDVEPLPAGHPLRIAPRL
                     LATPHLGYVSRANYTTYYGQAVEDIQAYLAGSPVRRLP"
     gene            complement(8657..9412)
                     /locus_tag="AMK32_RS36160"
                     /old_locus_tag="AMK32_35415"
     CDS             complement(8657..9412)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987974.1"
                     /locus_tag="AMK32_RS36160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_35415"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_073900629.1"
                     /transl_table=11
                     /translation="MPEQPPYLRIADELRRRIAEHEWEPGDRLPSRAQIGQEYGVGENV
                     VRRAQELLISQGVLEGRAGSGTYVAEPRQRVRVVRSSARERPDESPFRADMKAVGRQGD
                     WESRTEAKVPAPADIAARLGIAEGDLCVRTTYEFLADGRPVQLSTSWEPYDLTAGTLVV
                     LPEGGPHAGAGVANRMAAIGVTVSHAVEQPEPRQATAEEASLLGIQKAALVTHIRRTYY
                     SDQGRPVETADIVVPASHCEIVYEIPFNR"
     misc_feature    9442..9459
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.83"
                     /tool="antismash"
     misc_feature    9455..9466
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.35"
                     /tool="antismash"
     misc_feature    complement(9455..9470)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.6"
                     /tool="antismash"
     misc_feature    complement(9468..9482)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.48"
                     /tool="antismash"
     misc_feature    complement(9485..9498)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.49"
                     /tool="antismash"
     misc_feature    9488..9501
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.32"
                     /tool="antismash"
     misc_feature    9489..9502
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.51"
                     /tool="antismash"
     gene            9624..10286
                     /locus_tag="AMK32_RS36165"
                     /old_locus_tag="AMK32_35420"
     CDS             9624..10286
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMK32_RS36165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AMK32_35420"
                     /product="hypothetical protein"
                     /protein_id="WP_073900631.1"
                     /transl_table=11
                     /translation="MTRSGPLFRSQTVEFRPDSAVGEEIANLRASHSDVGRIFDAVPSV
                     LGLTSLEAANETGAFGVTVRAELTEAMVPHDAPAGSQPITSYLTSLSLVGWQAGDTHVA
                     AGLARMIAEPVEGGGNRTIDRTVLLETTDYRRIVERTVQDGTVVVVDGWWDALRDCLVN
                     RCAGECTNAALECPPASWPVYLACLAGRCGGCLAGCVGCATCDCGWLCRVAFGCCHQ"
ORIGIN
        1 gccccggtgc cgtccccgcc ccgggccggt gccggggaga agcgggcggt gtcgatgccg
       61 ttcgggacga ccgtgtacgg ggcgcgcacc ccggcgcgca gtccggtcgc gcgctctgcc
      121 tcgctgacgc acaccacccg gtcggcccag cgcgctcccc ggcgctccca ttgcagggcg
      181 agggcggcgg tgggtccgcc gacggcctcg aacgaccatg cgtgcggctg gaacacggtc
      241 ggtacccgcc cgcggacggc cagccggccg gccaggcccg ccttcgcgct gtgcgcgtgc
      301 accagttcgg gccgtacctc gtcgatcagg cgtgcgagcc gccgtacctc tcccggcagg
      361 cccggacccg gggaccgggc ggcccgccag tcccggacgt ccgcccccag ttcgcgcagt
      421 cccgcgccga gttcgccgcc ggggcaggcc accgtgaccc gcaggcccgc cgcgagctgg
      481 gcccgcgcca gatccagtac gacccgggcg acaccgccgt cgacgggctg ggcgaggtgc
      541 aggacccgtg ctcggggatc gggtgctgac tggtgcatac gcgggttcct cgctcacggg
      601 ggcgctcggg acgtgcggtc ggggacgcgc ctcactgccg gacggccacc gggacgcgga
      661 gtgcgccggc ccacgccgcg tccccgtgaa aacgagccgc aagcccggct ggtcaccacc
      721 ccgccgcgaa gcggcgccgg gatcgaacac gacggcgcac cggccgggcg tgtcggcgtg
      781 cgccggaggg cggacgggag ggcgcgcgca cgcaccggcg ctcccggggg aggaagtggg
      841 caattgtgaa ggaatcgccc ggacttcctt ccgtaggcgg gagagcggct cccgggcaca
      901 tcactccatt ggcggcacaa cccccggggg tgccacgcgt tgacacagcg ccgggtatcc
      961 gcccggatgt tgtgtcgatc ccaaggagct cctctccatg tcgcgtatcg cgaagggcct
     1021 ggtcctgacc tccgccgccg tcgcggccgt cgccggtggt gccggtgtgg ccgccgccga
     1081 ctccggtgcc cacggcgctg ccgccaactc gccgggcgtg ctgtcgggca acgccgttca
     1141 ggttccggtc cacgtgccga tcaacgtctg cggcaacacc gtcgacatca tcgctctgct
     1201 gaacccggcg ttcggcaacg cctgctacag cgactgacgt cggggcgccc cccttcggcc
     1261 ggccggtcac cccgcgatct cctcgcgggg cggccggccg gttttgtgct cccccggatg
     1321 ggccaccctg cgcgttcgcg cggttgcagc gagtgcccgg tgaacggacg gtgggctcag
     1381 gtcccccagt tcaccggaag gaacactcat gcgtgctctg ccgccccggc gtctcgcgtc
     1441 cactgtcctc tgcgccgccg tcctggtcgg catcaccggc cccgccgtcg tcgccgccga
     1501 ctcgtcccgg gaacacgcgc gagccgcctc gcccgcgttc gcccccgcgg ccgagaagct
     1561 gctggcccag gtcagagccc tggaccacac cggtaccgtg cccaggccgg tgatcgacct
     1621 gctcgagcag tcgctggaga agggcaagct gcccgccgcc gaggcccgga gactgggcga
     1681 cgcggcgaag aaggccctcg cggaggccgc ggcgcacccg gcgtccgcgg tcgccccctc
     1741 ggcgacggcc acgcccaccg cccccgccgc gacgcccacg gcaccggcgg cctccaccgc
     1801 accgacgact ccggccgagc ccgcggccgc caccccggcg gcttcgacgc ccgccgcctc
     1861 gaccccggcg gccgccgcgt ccacccccgc cgcctccact ccggcctccg cctccgcccc
     1921 cgcctccgcc tccgcgtccg cgtccgcctc gccgagccag tccgtggcgc ctgacgtggc
     1981 ggccagcccc tcgcagtcgg tggccgcgaa gaaccgcgac gacgccaagc ccgcggcccg
     2041 cgacgccgtg gacgacctgc tcgccgcact ggacaaggcg atcgacaacc tggtcgacgc
     2101 catcaccgcc cagctcgacc aggtgctcgc ctcggccgac ggactggtcg acggcctcct
     2161 cgaactgatc gactcggtcc tcgggggcgt cccctccaca ctgccgtcgc cgctctccgc
     2221 cgccgcggcg gtgccggacg tggccgtacc gtcgctggcc ccggccgagg ccgtaccgtc
     2281 gctgcccgcc ctgccggcga cgagcacctc cgcggccccg gaactgccgg ccggctgacg
     2341 ccgtaccgcc ccgcgtcccg gtcgtcgcgc cctccgggtg tgccggccgg gacgccgaga
     2401 tgaggccggg tgagggctcc tcggggatct catatgtctt gtcacccgag ggtttccggc
     2461 ccggtcgcga ctcgttaggc acggcgccgg acattccccg gggtgacgtg agtcgccgat
     2521 gaaaggagca cgatgaagtc cctgaaggcc gccgtcgtcg tcgccggttc cgtggccctc
     2581 gccggcgccg ccgcgcccgc gttcgcgtcc accgcccacg acctgccgcc catgagcctc
     2641 aacggcgggc tggcctcgat cgccgagcac ccgcccaccg tccaggacct gatgccgctc
     2701 cagcaccagt cggacgcgct cgacaccgag aacaaggact ccgtgctgag caccgtcaag
     2761 gacgcgacga ccacgctcaa caagccgggc ctgctcggcg gtcttcccct gagctgatct
     2821 ccgcaccccg caccactggg ccggtaccgc agccgcggcg ccggcccgcg ccgtgtccgg
     2881 gacggcttcc ctcgttcgtg tggagcgccc cgggccactg ctccggacgg gtgagaagcc
     2941 gtcaggcggc cccacggccc gtcggtatgg tcgcgcggtg acctcagcga caagtgcaac
     3001 ccgtcccttc cgcgccgccg acctcggcac gctcgtcctg ctcgcctgga tggccaggaa
     3061 cgaggagggg gacggcagga caccctgtct cctcgcctgc tccctgggcg acggccccga
     3121 cggcccggag gccgcgtccg ccgctgtgga gcggctcctc ggcgacgtcg gactgaccgt
     3181 cggcggcgag cccgtcgacg gcaccacccg gccgggcctc aaggtcagca tgctcgtcgt
     3241 ggccgggcag atcgtcctgc agctgccgaa cgtcagcagc caggtcaccc cgcccgccga
     3301 gtgggtgcag gtggtcgaga agctcggtta cacctacttc atgttcacca cccggatctg
     3361 cccctccacc ggcccccgcc ggccggtcca gggacaggag ctggccgtgt tcgcgggctc
     3421 cccggagacc accgacgccg ccgcccacgt cttcctgccc gcccgcggcc tgcgcacctg
     3481 atgcaacgcc acgcgggcgg cggccgtgcc ttcgccctgc tgatcgtgct cacgggggcg
     3541 gcgggtctgc tcgcctcctg ggtcatcacc ctcgacgagt tcaagctgct ggagaacccc
     3601 gccttcgtgc cggggtgcag cctgaacccg gtggtgtcct gcggcagcgt gatgaagagc
     3661 gaccaggcct cggtgttcgg gttcccgaac cccatgctgg gtctggtggc ctacgggatc
     3721 gtcgtctgcg tcggcgtgag tctgctcgcc ggggcccgct tcccccgctg gtactggctg
     3781 ctcttcgagg cgggctgcct gttcgggatc ggcttcgtca cctggctcca gttcgagtcg
     3841 ctgtaccgga tcgacgcgct gtgcctgtgg tgctgcctgg cctggatcgc gacgatcctg
     3901 ctcttctggt acgtcacgtc cctctgcgtg cgccacggcc tcctgcccgc tcccgcctgg
     3961 ctgaggacgt tcttcgccga gttcacctgg gtgctcccgg tattgcacat cggggtgatc
     4021 ggcatgctcg tgctgacgcg ctggtgggac ttctggacga gctgactatt acggcacccg
     4081 ggggaattgt gcactggtgg cgttttcggc tcgttacgcg gtacggacgc cgaacctccc
     4141 gcatcctgaa agggaccgta cctgacatga agtcgaccac ccgaggaacc cttgccgccg
     4201 tcgtcacggg catcgctgcc accgtgggag ccgccggtcc cgcggcggcg tcgggcgcgg
     4261 tccccgtctc cgtgcctctg ggcggcgtct ccacggccct cgacatggag gtgccccacc
     4321 tcggcgcgca ggtgcccctg gtgaagccgg gcgcccccga gggaccccgg tacgtcacgg
     4381 gccggctgct ccccgagcgg accctgcccc agctgccggt cagcagcggc ctgcccggcg
     4441 tggacgccag gtccccgctg ccccacgtcc tcggtgacgg cggcttcgac cacgtcggcc
     4501 tcgacgcgcc cgcctccgac ctgcgcgcac tcgccccggg cctgtccgtg gacgcgcccc
     4561 tgaccgcacc cgacgcgggg aacttcggcc tcccgcggcc caagctgccc caggccggca
     4621 tcctcacccc ggtcctccag aacgtctccg acgcgaacct gcagtcgggg cccggcctgt
     4681 aggaagcgca gcgccctggg cccgtcgggc ccgccgcggg ccctggcccc ggtccgccga
     4741 gccgcgtccg gcacgggcgc accgacggca cccaggccgc cgcatcggcc gggcgccggc
     4801 tgctccgccg gccgttcccc ggcccgtctc ctggtgtgcc ccgccgcggt cccggggctc
     4861 gctccatcgg ccgtaccgcc gtgcggtatt cgcccgttag ccgtcgtcgt accaccggat
     4921 ggccctcgga cggtgactcc gcacggcctt cagcggttac cactccggac aagatctgta
     4981 atccggacga ggagtacgcg atggtcgtcg gtgccggtgg cgtggctgtg ggcacggaac
     5041 aacagcaggt gtcaggggga gcgggcagac cgtcgcggag ggaggtgacg cggggcctga
     5101 tcgcctcggc cgcggcgctc gcgctggcac cggtcgtcgc cgcctcccgg cccgtgcccg
     5161 tgaacgagga gcccgacgga gactccttcg acgagaccta ccggggccgc cgcatccagg
     5221 gcttcctcgt gccgaccggg gggcgcaagg ccaccgacgt cgaatggcag atcaccatcg
     5281 acggccgccc gctgcacctg atgcgccgcg ccgacggcag ctggctgagc atggtcgacc
     5341 actacacgtc gtacgcgacg ccactggagg cgacgcgcgc ggccgtggac cagatcggcc
     5401 cccgccagcg gctgcgcgac ctggcacccg gcccggtcgg cggggaacag ccgggcggcc
     5461 acatgcacat ggcgatgggg ggtcggcatg gcgtacgtgc gtaagaacgt ccgcaacctc
     5521 accgcgacgg agcggcggcg gttcgtcgac gccctgctgg ccctgaaacg gcgcggcgag
     5581 tacgacgagt tcgtgcgcat gcacatccag tactacaccg ccgacggcga ggacggtctg
     5641 cgcaccgccc acatggcccc gtccttcctg ccctggcacc gcaggttcgt gctggacctg
     5701 gagcgggcgc tgcggcggat cgactcctcg gtgacggtgc cgtactggga ctggacccgg
     5761 gaccgctcga cgacctccgt gccctggacg aaggacctgc tcggcggcaa cggccggcgc
     5821 tccgaccggc aggtcatgac cggtcccttc gcctacgcct cgggtcactg gaccatcaag
     5881 gagggcgtca cggacggccg gttcctcgcc cgggacctgg gccgctccgc ctcccccatc
     5941 cagttgccca ccgcgaagga gctccagggg gcgctggacg acccggtcta cgacgcctgg
     6001 ccctggaact cgacggtccg caagggcttc cgcaacaaac tggagggctg ggggtcgggt
     6061 tccggcagcg cgtcctggca caaccacaac cgggtgcacc ggtgggtcgg cggcgcgatg
     6121 ctcggcggcg cctcggtcaa cgacccggtg ttctggctgc accacgcctt cctggacctg
     6181 cagtggtacc gctggcagcg ggcccaccgc agccaccgct acctgcccgc ggagccgccc
     6241 ggcgcccgtg acgaccagca cggacgggtg gtggcccggc accagaaact cccgccgtgg
     6301 aacgacaccc cggaccagct cgaggacatg agcggggtct accgctacgc ctgagacgcg
     6361 aacgcgatcg tgatcgtgat cgtgatcgcg agctggaacg ggaagaggaa cgggaagggc
     6421 cccggcgctc ggagtgccgg ggcccttcct tcgcgtacgg ccggatcagt tgccgtagcc
     6481 gccgtcgtgg ctggggccgg ggtggccggg gatgctgccg ccaccgctgt ggttgaggca
     6541 gctgttgccg aaggcggggt tcagcaggcc gacgatgtcg accgtgttgc cgcagaggtt
     6601 gaccgggacg tggaccggaa cctggacggc attgccggac agcacgccgg gcgagccggc
     6661 ggccagacca tgggcacccg cgtcggccag agccagcccg gccccgccga ccaccacggc
     6721 accggtgccg agggcgacaa cggctgactt cgcgatgcga gacatcacgt tctccttttg
     6781 ttgctcggta aggcgcagcg gcaggccagc caccgcactc cccgttcaac ggcggcacac
     6841 ggacctggtc acggtgatcg gcgggggatc acccttttaa cggcagccac ccggcggcgg
     6901 cccgcgcacc ccaccccgcg cccggcgata ttcgggggcg gcgggcccgg acgttgacct
     6961 agcctccggc catggacacc gacaaccctt ccgagccggc cgagcggtcc cggcagctga
     7021 tcggtctgct cgccgaggag cggcggctgc gggccttcgc ggccgtggcg ctgggcgcgg
     7081 acagcgccgg ccgggtcgcc gaggcggccg ggctcacggc gaaggacacg gccctcgcgc
     7141 tgctcaggct gcgcgagcag ggcgccgtga cggaccgccc ggacggcgga ctcaccgtct
     7201 cctacggcct gttgcgcgag ctggcccgca ccgagcggtc cgcccggccg tccggggacg
     7261 ccgtactgga caccttcgtg cgcggcggcc ggctcgtccg gctgcccgcc cagtggaccc
     7321 gcaagaagca ggtgctgcgg cacatcgccg agcagagctt cgagccgggc gtggagtacc
     7381 cggagcgcgt ggtgaacgag cggctgcgga gctggtgcga ggacgccgac gacaccgacc
     7441 atgtgacgct ccgccgctac ctggtggacc tgggccatct gcgccggagc gacggggtct
     7501 acaagcgcgt gacggcggcc tgacggacga tcagggcagt cgccgtaccg gagagccggc
     7561 caggtaggcc tggatgtcct ccaccgcctg gccgtagtac gtcgtgtagt tggcccgcga
     7621 cacatagccg aggtggggcg tggcgagcag gcggggcgcg atgcgcagcg ggtggccggc
     7681 gggcagcggc tcgacgtcga agacatcgac gccggcgccc gcgatccggc cctcgtggag
     7741 cgccgcgagc agggcctcct ggtcgacgat ggccgcgcgg gaggtgttga tcaggtaggc
     7801 ggtcggcttc atcaccgcga gttcggcggc gccgacgaga ccccgggtgc ggtcgccgag
     7861 ggccaggtgc acggagacga agtcgctctc cgcgagcagt tcctccttcg aggcggccag
     7921 ttcgacacct tcctccttcg cgcgctcctc ggtcaggttc tggctccacg cgccgacccg
     7981 catgccgaag gcgagcccga cgcgggccac ccggctgccg atccggccga gcccgagcag
     8041 gccgagccgg ctgccgtgca ggtcggcgcc gacggtggac tgccaggggc cgcccgaccg
     8101 cagcgcggtg ctctcctcga cgacgccccg cgcgagaccc agcagcagcg cccaggtcag
     8161 ctcgaccggc ggggtccccg aactcgccgt accgcacacg gtgacgccgt gcgcccgtgc
     8221 ggcgtcgtag tcgatgacgc tgttgcgcat gccggaggcg acgagcagct tcaggcgggg
     8281 gaggcgggcg atcagggagc cggggaaggc gacgcgttcg cgcagggtga cgacgatgtc
     8341 gaagccggcg agggcctcgg cgagggcgtc ctccccgtcg aggtgctcgc gcagcgcgac
     8401 cacctcgacc cggtcctcga tcaccgacca gtcggccata ccggtcgcca cgccctggaa
     8461 gtcgtccagc accacacaac gcagccgcac ccgacctcac ccctcgctcc cgacggtccc
     8521 gggtgcgact gtaacggccg ggcgggaggg cgcggcgcgg ggggatgacg gcacggcagg
     8581 agagctccga ccgggtgatc ttcagccctg cggccctact cggcatgcca atctgaagcg
     8641 tcctgggtga gccgaactac cggttgaacg gaatctcgta gacgatctcg cagtgcgagg
     8701 cgggcaccac gatgtccgcc gtctcgacgg gccgcccttg atcgctgtag tacgtccgcc
     8761 ggatgtgcgt gacgagcgcg gccttctgga tgcccaggag cgatgcttcc tcggcggtcg
     8821 cctgtctcgg ctcgggctgc tccacggcgt ggctgacggt gacaccgatc gcggccatgc
     8881 ggttcgcgac tcccgccccg gcgtgaggcc ctccttccgg gaggacaacg agagtgccgg
     8941 cggtgaggtc gtacggctcc caactcgtcg acagctgcac aggcctgccg tcggccagga
     9001 actcgtacgt cgtccggacg cacaggtcac cctcagcgat gccgagccgc gccgcgatgt
     9061 ccgccggagc cggcaccttg gcctcggtcc ggctctccca atccccttgc ctgcccacgg
     9121 ccttcatgtc cgcccggaac ggagactcgt cgggccgctc ccgcgcggac gaacggacca
     9181 cccgaacccg ctgtcggggc tcggcgacgt acgtgcccga accggcgcgg ccctccagta
     9241 cgccctggga gatcagcagc tcctgagccc ggcggaccac gttctcgccg accccgtact
     9301 cctggccgat ctgggcgcgg gacggcagac ggtccccggg ctcccactcg tgctccgcaa
     9361 tccttcgccg gagttcgtcg gcgatgcgga gatagggcgg ctgctcaggc atgtggaaaa
     9421 tctaatccac tatctctaat ctagttaact agctgcaaat gcttactaat tgacggaaaa
     9481 acaatttgct tctaaacaaa aggacttgcg ttcctgttgg atgactcgac atgcgcatat
     9541 tgcagaatgg tccgatgatg gaaccgggac cccatcaata gggacaaccc ccaactcgac
     9601 atccatagag ccaaggaggg atcatgacca gaagcggacc gctcttcagg agccagaccg
     9661 tggagttcag gcccgacagc gcagtggggg aagaaatcgc gaatctcagg gcatcccact
     9721 ccgacgtggg ccgcatcttt gacgcagttc cgtccgttct cggcctgact tccctagaag
     9781 cagccaacga gacgggtgcc ttcggcgtca cggtgcgagc ggaactcacc gaggcgatgg
     9841 tcccgcacga cgcgccggcc ggatcgcagc caatcacgtc ctacctcacg tcgttgtccc
     9901 tcgtcgggtg gcaagctggg gatacgcacg tggcagctgg ccttgctcgc atgatcgccg
     9961 aacctgtcga aggcggcggc aaccgcacca tcgaccgcac cgtcctcctg gagaccaccg
    10021 actaccggcg catagtcgag cgcaccgtgc aggacggtac cgtggtcgtg gtggacggct
    10081 ggtgggacgc tctgcgcgac tgcctcgtca accgatgtgc gggtgagtgc acgaacgcgg
    10141 ccctggagtg tccgcccgcg tcctggcccg tctacctggc ctgcctcgcc ggccggtgtg
    10201 gcgggtgcct tgcagggtgc gtgggatgcg caacctgcga ctgcggctgg ctgtgccgag
    10261 tggccttcgg gtgctgccac cagtagccgc ggagggcaga gacatggtca cctggctgga
    10321 gatgccgctc cgctcctccg gagcgcaacc ctctctccga gcaaagtacg acgtcggcaa
    10381 tgcagtggtc atgctgagcg agacaacggc atacgtcgcg ctcgcagacc cgaaagctgc
    10441 aaacgaactg ctgcagcacc ccgacgtcca acgcctcacc gccaacaacc tcaagacgat
    10501 ccag
//
