LOCUS       NZ_LIWA01000006        11359 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB01883 scaffold6, whole genome shotgun sequence.
ACCESSION   NZ_LIWA01000006
VERSION     NZ_LIWA01000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB01883
  ORGANISM  Streptomyces sp. CB01883
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 652158)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652158)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905855.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:34:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,089
            CDSs (total)                      :: 8,009
            Genes (coding)                    :: 7,788
            CDSs (with protein)               :: 7,788
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 85 of 221
            Pseudo Genes (incomplete)         :: 169 of 221
            Pseudo Genes (internal stop)      :: 25 of 221
            Pseudo Genes (multiple problems)  :: 50 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:33:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 275811
            Orig. end                         :: 287170
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIWA01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11359
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11359
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11359
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6359](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6359
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(95..107)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
     misc_feature    210..224
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.36"
                     /tool="antismash"
     misc_feature    complement(284..298)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.08"
                     /tool="antismash"
     gene            825..2351
                     /locus_tag="AMK32_RS27485"
                     /old_locus_tag="AMK32_26985"
     CDS             825..2351
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445328.1"
                     /locus_tag="AMK32_RS27485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_26985"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_073897877.1"
                     /sec_met_domain="DAO (E-value: 2.1e-45, bitscore: 150.7,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHEQRRVTDSYWLTTARGPSRPPLTEDVDVDVAVVGAGVAGISTA
                     HELTRAGLRVAVLEAGRVAGGVTGFTTAKLTAQHTLVYDRLRRTRGPEGARLYARSQSE
                     AIEHAAMLVAELDIDCEWEVRDAYTYVRDRERAEEVRAEAEAAREAGLPATFVTRTGLP
                     FPVAGAVRVAEQAQFHPVKYLRALTADLVARGGRVYEETRVVGLQEGGEPCRLTTEAGH
                     TVTARDVVIATHYPVFDRALLFARLSPRRELVVAGPLGDGPDPAGMYITPEENTRSVRT
                     APYGADGRRLLVVTGEHFTPGTGDPRAGFDRLAGWAEVHFPGVKLDHAWATQDNESTDT
                     VPLVGPFHPGARHTYVATGFGGWGLSGGIMAGLLLTALITGGDSAWRELYDPRRLKSVV
                     REAPALLKTQAEVARHFVGDRLKPAASVDDLAPGDGALVRAGGERLAVHRDDEGDLHAV
                     SARCTHLGCLVSFNRAERAWECPCHGSRFGVDGSVLQGPAVKPLEQHDLG"
     gene            2445..3293
                     /locus_tag="AMK32_RS27490"
                     /old_locus_tag="AMK32_26990"
     CDS             2445..3293
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675664.1"
                     /locus_tag="AMK32_RS27490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_26990"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_073897415.1"
                     /transl_table=11
                     /translation="MIPLARRLTAVCALGAALTACGTAGSTGTAHPAKSSASSSPSPAR
                     PPVLAPGPGGLTPVFEHGPRTRGKTVALTFDADMTADEGRRAASGEHFDNPGLIAALRS
                     LKVPATVFMTGRWADQYPAEARSLGHEAQFEVANHSYSHYAFTEDCYGLPTVGADRMRS
                     DVKRAYTSLRKAGVQKPMPYFRFPGGCYDQQALRALSGLGVTAVQWDVVSGDAFATDAD
                     AVVRQVLDGVKPGSVVVMHCTRSAAPTTERVVRSVVPELRKRGYRFVKVSELIGESGGN
                     R"
     gene            3312..3494
                     /locus_tag="AMK32_RS27495"
                     /old_locus_tag="AMK32_26995"
     CDS             3312..3494
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675663.1"
                     /locus_tag="AMK32_RS27495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_26995"
                     /product="hypothetical protein"
                     /protein_id="WP_073897417.1"
                     /transl_table=11
                     /translation="MSDEDYCTIVDATKPPRADGPPYAECVLCQKPTEYPESYRGITLC
                     PVCEWQEAQRTACSG"
     gene            complement(3534..3974)
                     /locus_tag="AMK32_RS27500"
                     /old_locus_tag="AMK32_27000"
     CDS             complement(3534..3974)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675660.1"
                     /locus_tag="AMK32_RS27500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_27000"
                     /product="DUF6086 family protein"
                     /protein_id="WP_073897419.1"
                     /transl_table=11
                     /translation="MSQYFDIGDETLWNPSDGAARLFLRQVAVFEAELGLPSGLGRMEN
                     DECQVDPVAFGVFTNSLLARHRRTGHSVLLALSEGFVATVLVLAGRAGVPVDWAGLEAV
                     PDGPLRDVQASARTGMSGPAEGGTWAAELRARAAELGRGMPR"
     gene            4145..4861
                     /locus_tag="AMK32_RS27505"
                     /old_locus_tag="AMK32_27005"
     CDS             4145..4861
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657150.1"
                     /locus_tag="AMK32_RS27505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_27005"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_073897421.1"
                     /transl_table=11
                     /translation="MSHVPPSGDSPFQADRTPRDEAPQFVLPLVVRIERDAPPARTDAL
                     ETAARAVLLLLGDARAHGDGEWAEAVRNWEDARIRKVVRRARGAEWRRAEALPGITVTG
                     KSAEVRVFPPVPLDGWPKDLARLQVSGTELDDPEPPAAADPGEPVLWLNPELSMSAGKA
                     MAQAGHGAQLAWWALSDAERAAWREAGFPLSVRTAQPADWARLTGGGLPLVRDAGFTEI
                     APGSCTVVADHPALHR"
     misc_feature    4168..4182
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.86"
                     /tool="antismash"
     gene            5001..6359
                     /locus_tag="AMK32_RS27510"
                     /old_locus_tag="AMK32_27010"
     CDS             5001..6359
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675658.1"
                     /locus_tag="AMK32_RS27510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_27010"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_073897423.1"
                     /sec_met_domain="DUF692 (E-value: 1.3e-97, bitscore: 321.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIADAVERMPGIDWVEAVAENLCPGHLPESLRR
                     LRERGVTVVPHGVSLGLGGADRPDEGRLTALAERAEALGAPLVTEHIAFVRAGGALTAS
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQEALPVPLAVENIAALFSWPGEELTEGQFLS
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAQALAALPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPAPVLDLLTDLASRVAPPGVLLERDENFPEPAELERELTAIGDAVGEGRARAADG
                     RAPAAGPPAGSAGSAAAGPADGGVAVLTRTATEVRAREATRERLGVAQAAVLSALVAGT
                     PVPEGFDRVRMGVQARALAAKRAGVVARVAPELPEILGAGYRPAFLEYARTRPMSGGYR
                     RDALDFAGHLLRTAPPAGAAARRELRNWWLDRAGPAPRPRGPVARARALLRRG"
     gene            6523..7266
                     /locus_tag="AMK32_RS27515"
                     /old_locus_tag="AMK32_27015"
     CDS             6523..7266
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073954.1"
                     /locus_tag="AMK32_RS27515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_27015"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_073897425.1"
                     /transl_table=11
                     /translation="MRPRPPLKGRGIFSGTGLIITGLAATVVALLVPLWSYADRPDTAS
                     AGVLSARTVTTPFGPLSEQDRDFITKVRLAGLWELPAGELAEREGTSPAVRTAGTHLVD
                     GHSSLDAHVRTVATQLGVPLPNEPNEQQKQWLATLGAARGQDFDRRFAGILRLAHGRVF
                     SLVAQVRAGTQNSLVRDLADDANATVLDHIKILEATGYVDFAALSRDLAASAPPVPPPP
                     AVAPGAAVPVTPPPSVTGAPPTSSP"
     gene            7366..8145
                     /locus_tag="AMK32_RS27520"
                     /old_locus_tag="AMK32_27020"
     CDS             7366..8145
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675656.1"
                     /locus_tag="AMK32_RS27520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_27020"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073897427.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAFAGLACTRLCLAAVRAAASDAGGTAPDGAHALT
                     LYEAAFLSGGPARVADVTLVSMARQRRLLLAHTGWATVVDPRGRDEMERSVIGAIGPEG
                     QSRIAPVRARAAGAESVRRLADGLVRAGLAVPDGSGATVGAAVRQVRAAALTVVVLGAA
                     ALALPVQTGTPRLLVALWFALPLTLSLSCLAIARFEVHPYSRWASPAGQRLLGALTRGP
                     AGDERSFLTSVAVRGTRAIGEPELRAAFAHRDQPWRE"
     gene            8295..9959
                     /locus_tag="AMK32_RS27525"
                     /old_locus_tag="AMK32_27025"
     CDS             8295..9959
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (117-490). E-value:
                     8.2e-21. Score: 66.6. Matches aSDomain:
                     nrpspksdomains_AMK32_RS27525_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675655.1"
                     /locus_tag="AMK32_RS27525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_27025"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_073897429.1"
                     /sec_met_domain="PF00561 (E-value: 2.5e-26, bitscore: 87.8,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYSAAGSLLLAGLAAAPAGGAPAAPGAPDASGAAELRGTA
                     VAAARARAAGVDFGACSAADVPEAPGGLRCGTVSVPLDYAHPDGKQIRLTVSRMRASQK
                     DPHNSKRKVPRQGVLVYNPGGPGASGMYFPLIGMVPEWKRIAAAYDLVGYAPRGVGRSA
                     PLSCEDPRRYFKAPTASPTHPSEAYKRERVAAAKAYARGCAQRSGSGLRHYTSLNNARD
                     LDVLRAALGEDRLTFMGASYGTYFGALYASMFPSHVRRMVLDSAVDPDPAKIWYRSNLD
                     QSAAFEERWTDFREWIARHDDAYRLGNTPAKVQRGYDTARERLAGKAAGGKVGPGQLQN
                     AFLAAGYYDDFWPSRAAALSAYLHGDPQPLVRLAAPMAETAAEAENSSAVYTAVECNDA
                     PWPTDFAVWDRDNTRLARVAPFETWDNAWLNLPCAYWQAPRRQPVDVRTGPGELPPVLI
                     LAAERDAAAPYSGSVEMQRRLAGSVLVTERDAGTHGVAGGPNRCVNAYFDAYLLEGRLP
                     ARHASCAPRQEPKPDASGGRTAVEVRRDGVKGSSGRR"
     aSDomain        8646..9764
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK32_RS27525_Abhydrolase_1.1"
                     /evalue="8.20E-21"
                     /label="AMK32_RS27525_Abhydrolase_1.1"
                     /locus_tag="AMK32_RS27525"
                     /protein_end="490"
                     /protein_start="117"
                     /score="66.6"
                     /tool="antismash"
                     /translation="VLVYNPGGPGASGMYFPLIGMVPEWKRIAAAYDLVGYAPRGVGRS
                     APLSCEDPRRYFKAPTASPTHPSEAYKRERVAAAKAYARGCAQRSGSGLRHYTSLNNAR
                     DLDVLRAALGEDRLTFMGASYGTYFGALYASMFPSHVRRMVLDSAVDPDPAKIWYRSNL
                     DQSAAFEERWTDFREWIARHDDAYRLGNTPAKVQRGYDTARERLAGKAAGGKVGPGQLQ
                     NAFLAAGYYDDFWPSRAAALSAYLHGDPQPLVRLAAPMAETAAEAENSSAVYTAVECND
                     APWPTDFAVWDRDNTRLARVAPFETWDNAWLNLPCAYWQAPRRQPVDVRTGPGELPPVL
                     ILAAERDAAAPYSGSVEMQRRLAGSVLVTERDA"
     gene            complement(9961..10692)
                     /gene="hemQ"
                     /locus_tag="AMK32_RS27530"
                     /old_locus_tag="AMK32_27030"
     CDS             complement(9961..10692)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="AMK32_RS27530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_27030"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_073897431.1"
                     /transl_table=11
                     /translation="MSDDASTTESGRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADLMIWWHAETSDQLQEAYNLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWVLAFEADELYRIVDLMRHLRGSEARRHVR
                     EEVPFYTGRRKSVAELVAGLA"
ORIGIN
        1 ggcctgacgc tggtcatccc cttcgtcgac cggctgcaca agatcaacat gcagatcgtg
       61 acgctgccga tccccgccca ggagggcatc acccgggaca acgtcaccgt ccgcgtggac
      121 gcggtcgtct acttccgggt gatggacccg acgagcgcgc tggtgaaggt cgaggactac
      181 aagttcgccg tgtcgcagat ggcccagacc tcactgcggt cgatcatcgg caagagcgac
      241 ctcgacgacc tgctctccaa ccgggagaag ctcaaccagg gcctggaact gatgatcgac
      301 agcccggccg tgggctgggg catccaggtg gaccgggtgg agatcaagga cgtctccctg
      361 ccggacacga tgaagcggtc catggcccgc caggccgagg ccgaccggga gcgccgggcc
      421 cggatcatca acgccgacgc cgagctccag gcctccaaga aactcgcgga ggccgcccag
      481 cagatgtccg gcacgccgac ggcgctccag ttgcggctgc tccagacggt gatggcggtg
      541 gcggccgaga agaactccac gctggtcctg cccatcccgg tggaactgct gcacttcctg
      601 gaacggggcg ggcagcaggg cgctccggag accggcgggg ggcggcgcga ggcaccgagg
      661 gaggcgccgc gggaggttgc cgccctcccg gacagcctgc cggaggggct cgcggaggag
      721 cccgccccca acggcggcgg aggggcacgc ccgccgggac aggggccgcg ggaacggctg
      781 ccgaagcaga cgtgagcgcc gcccgctggg ggtacccggc gggcatgcac gagcagcgca
      841 gggtcaccga ctcgtactgg ctgacgaccg cccgcgggcc gtcccgtccc ccgttgaccg
      901 aggacgtcga cgtcgacgtg gcggtcgtcg gcgcgggcgt ggccggcatc agcaccgccc
      961 acgaactgac ccgtgccggc ctgcgggtgg cggtcctgga ggccggccgg gtcgccggcg
     1021 gggtcaccgg cttcaccacc gccaagctga ccgcgcagca cacgctcgtc tacgaccggc
     1081 tgcgccgcac gcgcgggccc gagggggcgc ggctgtacgc ccgttcgcag tccgaggcga
     1141 tcgagcacgc ggcgatgctg gtggccgagc tggacatcga ctgcgagtgg gaggtgcggg
     1201 acgcctacac ctacgtgcgg gaccgggagc gcgccgagga ggtgcgggcc gaggcggagg
     1261 ccgcgcggga ggcggggctg ccggccacgt tcgtgaccag gaccgggctg ccgttcccgg
     1321 tggcgggcgc ggtccgggtc gccgagcagg cccagttcca cccggtcaag tacctgcggg
     1381 cgctcaccgc cgacctcgtc gcgcgcggcg ggcgggtgta cgaggagacg cgggtcgtcg
     1441 gactgcagga gggcggggag ccctgccggc tgacgacgga ggcgggtcac acggtgaccg
     1501 cccgggacgt cgtgatcgcc acccactatc cggtgttcga ccgggctctg ctgttcgccc
     1561 ggctgtcgcc ccgccgggaa ctcgtcgtcg cggggccgct gggcgacggc ccggacccgg
     1621 cgggcatgta catcaccccc gaggagaaca cccgctcggt gcgcaccgcg ccgtacggcg
     1681 cggacgggcg gcggctgctc gtcgtcacgg gcgagcactt caccccgggc accggcgatc
     1741 cgcgcgcggg cttcgaccgg ctcgccggct gggccgaggt gcacttcccc ggcgtgaagc
     1801 tcgaccatgc ctgggccacc caggacaacg agtcgaccga caccgtcccg ctggtcggcc
     1861 ccttccaccc gggcgcccgg cacacctacg tggccacggg cttcggcggc tggggcctga
     1921 gcggcggcat catggccggt ctgctgctga ccgcgctgat caccggcggc gacagcgcct
     1981 ggcgcgagct gtacgacccg cgccggctga agtccgtggt gcgcgaggca ccggcgctgc
     2041 tgaagacgca ggcggaggtg gcccggcact tcgtcggcga ccggctgaag ccggcggcgt
     2101 ccgtggacga tctggcaccg ggggacggcg cgctggtccg ggccggcggc gagcggctcg
     2161 ccgtgcaccg cgacgacgag ggcgacctgc acgccgtgtc ggcccgctgc acccacctgg
     2221 gctgcctggt ctccttcaac cgggccgagc gcgcctggga gtgcccctgt cacggctccc
     2281 gcttcggcgt ggacggctcg gtgctccagg gaccggcggt caagccgctg gagcagcacg
     2341 atctcggctg accggtggcc gacggcccgc cgcgcgggtg accgtaccgc cgcgccacgg
     2401 gacatatcac catacaaacg ggcattcagt cctacgttcg tccggtgatc ccacttgcac
     2461 gccgtctcac cgccgtctgc gccctgggcg cggccctcac cgcctgcggc accgcgggct
     2521 ccacgggtac cgcgcacccg gccaagtcgt ccgcgtcctc ctccccgtcc ccggcccgcc
     2581 cgccggtgct cgccccgggt ccgggcggtc tgaccccggt cttcgagcac ggtccgcgca
     2641 cccggggcaa gaccgtcgcg ctgaccttcg acgccgacat gaccgccgac gagggccgcc
     2701 gcgcggcctc cggcgagcac ttcgacaacc cgggcctcat cgccgccctg cgttcgctga
     2761 aggtgccggc gaccgtcttc atgacgggcc gctgggccga ccagtacccg gccgaggcgc
     2821 gcagcctggg ccacgaggca cagttcgagg tcgccaacca ctcctacagc cactacgcgt
     2881 tcaccgagga ctgctacggc ctgccgaccg tcggcgcgga ccggatgcgg tcggacgtca
     2941 aacgggcgta cacctccctg cgcaaggcgg gcgtgcagaa gccgatgccg tatttccgct
     3001 tcccgggcgg ctgctacgac cagcaggccc tgcgcgcgct gagcgggctc ggggtgacgg
     3061 ccgtgcagtg ggacgtggtg agcggtgacg cgttcgccac ggacgccgac gcggtggtcc
     3121 gccaggtcct ggacggggtg aagcccggct cggtggtcgt catgcactgc acccgcagcg
     3181 ccgccccgac gaccgagcgg gtcgtccggt ccgtggtgcc ggaactgcgc aagcgcggct
     3241 accgcttcgt caaggtctcc gagctgatcg gcgagagcgg cggtaaccgc tgagcgtcct
     3301 acgctgggag catgagcgac gaggactact gcacgatcgt cgacgccacg aagccgccga
     3361 gggccgacgg gccgccgtac gcggagtgcg tgctgtgcca gaagccgacg gagtacccgg
     3421 agtcgtaccg gggcatcacg ctctgcccgg tgtgcgagtg gcaggaggcg cagcgcaccg
     3481 cctgctcggg gtgaccggcg ggcggcgcgg cccccgggcg cggccgcggc cggtcagcgg
     3541 ggcatgcccc ggcccagctc cgccgcccgg gcccgcagct ccgccgccca cgtcccgccc
     3601 tcggccggtc cggacatccc cgtacgggcc gaggcctgga cgtcccgcag cgggccgtcc
     3661 gggacggctt cgagccctgc ccagtccacc gggacgcctg cgcgcccggc gaggaccagc
     3721 acggtggcga cgaacccctc ggacagggcc agcaggacgg agtgtccggt gcggcggtgc
     3781 cgggcgagga gggagttcgt gaagacgccg aaggcgacgg gatcgacctg gcactcgtcg
     3841 ttctccatgc gcccgaggcc cgacggcagc cccagctccg cctcgaagac ggccacctgg
     3901 cgcaggaaga gccgcgcggc cccgtcggag gggttccaca gcgtctcgtc accgatgtcg
     3961 aagtactgac tcattcccgg tcccccgccc gtcggtccgt cgtggaccgc ccctcgatcc
     4021 gtcgtggatc aaaagttgat caagggagca gagtagtcgg ccggtccggt gcggggcgcc
     4081 ggaattccgt cgccggggaa cgcgcgttga tcggcgggcc ggcggcttcg tagggtgggc
     4141 tgtcgtgagc cacgttcccc cctccggtga cagtcccttc caggccgacc gcactccccg
     4201 cgacgaggca ccgcagttcg ttctgccgct cgtcgtgcgg atcgaacgcg acgcgccgcc
     4261 cgcccgcacc gacgcgctgg agacggccgc ccgtgccgtg ctcctgctgc tcggcgacgc
     4321 acgggcgcac ggcgacggcg agtgggccga ggccgtccgg aactgggagg acgcccggat
     4381 ccgcaaggtc gtgcggcggg cccgcggtgc cgagtggcgg cgcgccgagg cgctgccggg
     4441 gatcacggtg accggcaagt ccgcggaggt ccgcgtcttc ccgccggtcc cgctggacgg
     4501 ctggcccaag gacctggcca gactccaggt gtcgggcacg gagctggacg acccggaacc
     4561 gcccgccgcc gcggacccgg gcgagcccgt gctgtggctc aacccggagc tcagcatgtc
     4621 ggccggcaag gcgatggccc aggccgggca cggcgcccag ctggcctggt gggcgctgtc
     4681 cgacgccgag cgcgcggcct ggcgggaggc cggcttcccg ctctccgtcc gcaccgccca
     4741 acccgccgac tgggcccggc tgaccggcgg cgggctgccc ctggtccggg acgccggttt
     4801 cacggagatc gcaccgggct cctgcacggt cgtcgccgac cacccggcgc ttcaccgctg
     4861 aggcgagccg gccgggaccc cgcgccggaa cctcaaatgt tgctctgaac acgccatcgg
     4921 cggggttcgg ggccgcccgc cggggccata cccccgtcac tcggcgcacg aggttcgcgc
     4981 gaggaggagg gggaggcgcc atgcggcgac tgggcacggg gatcggctgg cggccggaga
     5041 tcgcggacgc cgtggaacgc atgccgggca tcgactgggt ggaggcggtg gcggagaacc
     5101 tctgcccggg ccacctcccc gagtcgctgc ggcggctgcg cgagcggggc gtgacggtcg
     5161 tcccgcacgg cgtctccctc ggcctgggcg gcgcggaccg ccccgacgag ggccggctga
     5221 cggcgctggc cgagcgggcg gaggcgctcg gcgcccccct ggtcaccgag cacatcgcgt
     5281 tcgtccgggc gggcggtgcg ctcaccgcgt ccccgcgtct cgaagcgggc catctgctgc
     5341 ccgtgccgcg tacccgggac gccctcgacg tgctgtgcga gaacgtacgc atcgcgcagg
     5401 aggcgctgcc ggtgccgctc gccgtggaga acatcgccgc cctgttctcc tggccgggcg
     5461 aggagctgac cgagggacag ttcctgtccg aactggtcga gcggaccggg gtacggctcc
     5521 tcatcgacgt ggccaacctg cacaccaacc acgtcaaccg gggcgaggac ccggcgcagg
     5581 cgctggccgc gctgccgctg gaggcgatcg cctacgtcca tgtcgccggc ggcttcgagc
     5641 gggacggcgt ctggcacgac agccacgcgc acccggtgcc ggccccggtc ctcgatctgc
     5701 tgaccgacct cgcctcccgg gtcgccccgc cgggcgtcct gctggagcgc gacgagaact
     5761 tcccggaacc ggcagagctg gagagggagt tgaccgcgat cggggacgcg gtcggcgagg
     5821 ggcgcgcccg ggcggccgac ggccgggctc ccgcggccgg cccaccggcc ggctcggcgg
     5881 gcagcgcggc ggccggtccg gcggacgggg gtgtcgcggt cctcacgcgg acggcgacgg
     5941 aagtgcgcgc ccgggaggcg acccgggaac ggctgggggt cgcgcaggcc gccgtactgt
     6001 ccgcgctggt cgccgggaca ccggtgccgg agggcttcga ccgggtgcgg atgggggtgc
     6061 aggcgcgggc gctcgcggcg aaacgggccg gcgtggtggc gagggtggcc cccgagctcc
     6121 cggagatcct gggcgcggga taccggccgg cgttcctgga gtacgcccgg acgcggccga
     6181 tgagcggggg ctaccggcgg gacgcgctgg acttcgccgg ccatctgctg cggaccgcgc
     6241 cgcccgccgg ggcggcggcc cgccgggagc tgcggaactg gtggctggac cgggcgggcc
     6301 cggccccccg cccccgcggc ccggtggcac gggcacgggc gctgctgcgg aggggctgag
     6361 acccgcgggg ggcggccggg cggggccggt gcgtccgagg gcacgggcgg ggggcgtgac
     6421 gccccggggg cgcgggccgg gtccgcgccc ccagtaatat gccaagccgc acccacccac
     6481 cctcaagcac ccctggtgtg cggtgccgta tcaggaggcg ccatgcgacc gcgacccccg
     6541 ctcaagggcc gaggcatatt cagcggtacc gggctcatca tcacgggcct ggcggcgacg
     6601 gtcgtggcgc tactcgtccc gctctggtcg tacgccgacc ggccggacac cgcctccgcg
     6661 ggcgtgctga gcgcacggac cgtcaccacc ccgttcggcc cgctgtccga gcaggaccgg
     6721 gacttcatca ccaaggtccg gctcgccggc ctgtgggagc tgcccgccgg cgaactggcg
     6781 gagcgcgagg ggaccagccc ggccgtccgc accgccggaa cgcacctcgt cgacggccac
     6841 agctccctcg acgcccatgt gcggaccgtc gccacccagc tcggcgtccc gctgcccaac
     6901 gagccgaacg agcagcagaa gcagtggctg gccaccctgg gcgcggcccg cgggcaggac
     6961 ttcgaccgcc ggttcgcggg catcctgcgc ctggcgcacg gccgggtgtt ctcgctggtc
     7021 gcccaggtgc gggcgggcac ccagaactcc ctggtgcgcg acctcgccga cgacgccaac
     7081 gccaccgtcc tcgaccacat caagatcctg gaggcgacgg ggtacgtcga cttcgccgcg
     7141 ctgtcccggg atctcgccgc ctccgcgccc ccggtccccc cgcccccggc cgtcgcgccg
     7201 ggcgccgccg tcccggtgac gcccccgccc tcggtcaccg gcgcaccgcc gacgagcagc
     7261 ccctgacctg cgcgggaaac ggaacgtcac gtacccgcaa cgctcggagt tcgaatactg
     7321 aacagggcat ggcgttcgca cgggcccctg gcatacaaac acgtcatgtt ctgggtcctt
     7381 cttctccttc tggcctgggc gttcgccggc ctcgcgtgca cccggctgtg cctggcggcg
     7441 gtccgcgcgg cggcctcgga cgcgggcggc accgcaccgg acggggcgca cgcgctgacg
     7501 ctgtacgagg cggccttcct gtccggcggc ccggcccggg tcgccgacgt caccctggtc
     7561 tcgatggccc ggcagcggcg gctgctgctc gcgcacacgg gctgggcgac agtggtcgac
     7621 ccgcggggcc gtgacgagat ggaacggtcc gtcatagggg cgataggccc cgagggtcag
     7681 tcccgcatcg ccccggtgcg ggcccgcgcg gccggtgccg agtccgtgcg ccggctggcg
     7741 gacggactgg tgcgcgcggg tctcgcggtg cccgacggct cgggtgcgac cgtgggcgcg
     7801 gccgtgcgcc aggtgcgggc cgccgcgctg accgtcgtcg tgctgggcgc ggccgcgctg
     7861 gcgctgcccg tccagacggg gacgccccgg ctgctggtcg ccctgtggtt cgcgctgccc
     7921 ctgacgctgt cgctgagctg tctcgccatc gcccggttcg aggtgcaccc gtactcgcgc
     7981 tgggcctccc cggccggtca gcggctgctg ggcgcgctca cccggggtcc ggccggcgac
     8041 gagcggtcgt tcctcacctc ggtcgccgtg cgcggcaccc gcgcgatcgg cgagccggaa
     8101 ctgcgcgcgg ccttcgccca tcgcgaccag ccctggcggg agtgagggca ccctcggggg
     8161 cgcacagggg gcattgacgc cgacaccggc cgggtggtgc ttgccttcac cgacggccgc
     8221 ccgaaacatc ccttgttggt gtcgccgggc cgtggcagcc gtgccatcgc cgccgcgcac
     8281 cggagggaaa cgcgatgaga gctgccgccc tgtactcggc cgccgggtcc ttgctgctgg
     8341 ccgggttggc cgcggccccc gcgggcggcg cgcccgccgc tcccggggcg cccgacgcct
     8401 ccggcgcggc cgaactgcgc ggcaccgccg tggccgcggc gcgcgcccgg gcggccggtg
     8461 tcgacttcgg cgcgtgctcc gccgccgacg tcccggaggc gcccggcggc ctgcgctgcg
     8521 gcacggtgag cgtcccgctg gactacgccc acccggacgg caagcagatc aggctcaccg
     8581 tcagccggat gcgggcctcg cagaaggacc cccacaacag caagcggaag gtcccccggc
     8641 agggtgtcct cgtgtacaac ccgggcggtc ccggcgcctc cggcatgtac ttcccgctga
     8701 tcggcatggt cccggagtgg aagcggatcg ccgccgcgta cgacctggtc ggctacgcgc
     8761 cgcgcggcgt cggccgttcc gcgccgctgt cctgcgagga cccccggcgg tacttcaagg
     8821 cgcccaccgc gtcgccgacg catccgtcgg aggcgtacaa gcgcgaacgc gtcgcggcgg
     8881 cgaaggcgta cgcgcgcggg tgcgcccagc ggtcgggcag cgggctgcgc cactacacct
     8941 ccctcaacaa cgcccgtgac ctcgacgtcc tgcgggccgc gctgggcgag gaccggctga
     9001 cgttcatggg cgcgtcgtac ggcacctact tcggcgcgct gtacgcgtcg atgttcccct
     9061 cgcacgtgcg gcggatggtg ctggactcgg cggtcgaccc ggacccggcg aagatctggt
     9121 accgcagcaa cctggaccag tcggccgcgt tcgaggagcg ctggacggac ttccgcgagt
     9181 ggatcgcccg ccacgacgac gcctaccggc tcgggaacac gcccgcgaag gtgcagcgcg
     9241 gttacgacac cgcgcgggag cggctggccg ggaaggcggc gggcggcaag gtcgggccgg
     9301 gccagctgca gaacgcgttc ctggcggccg ggtactacga cgacttctgg ccgagccggg
     9361 ccgcggccct gtcggcgtac ctgcacgggg atccgcagcc gctggtccgg ctggcggccc
     9421 cgatggcgga gacggccgcg gaggcggaga acagcagcgc ggtgtacacg gccgtggagt
     9481 gcaacgacgc gccctggccc acggacttcg ccgtgtggga ccgcgacaac acccggctcg
     9541 cccgggtcgc gcccttcgag acctgggaca acgcctggct gaacctgccg tgcgcctact
     9601 ggcaggcacc ccgccggcag ccggtcgacg tgcgcaccgg cccgggtgag ctgccgccgg
     9661 tgctgatcct ggcggccgag agggacgcgg ccgcgcccta ctcgggttcc gtggagatgc
     9721 agcggcggct ggccggctcg gtgctggtga ccgagcggga cgcgggcacg cacggcgtcg
     9781 ccggcggccc gaaccgctgc gtcaacgcct acttcgacgc ctacctgctg gaggggcgcc
     9841 tcccggcgcg gcacgcgtcc tgcgcgccgc gccaggaacc gaagccggac gcctccggcg
     9901 gacggaccgc cgtggaggtc cggcgggacg gcgtcaaggg cagcagcggc cggcgctgac
     9961 tcaggccagg ccggcgacca gctccgccac cgacttgcgg cggccggtgt agaagggcac
    10021 ctcctcgcgg acgtgcctgc gggcctcgga gccgcgcagg tgacgcatga ggtcgacgat
    10081 gcggtacagc tcgtcggcct cgaaggccag cacccactcg tagtcgccga gggagaacga
    10141 ggcgaccgtg ttggcgcgca cgtccgggta gccgcgggcc atcttgccgt ggtcggcgag
    10201 catgcggcgg cggtcctcgt cggggagcag gtaccagtcg taggagcgca cgaaggggta
    10261 gacgctcacg tagttgcggg gcgtctcgtc ggcgaggaac gccgggatgt gcgagcggtt
    10321 gaactcggcg gggcggtgca gcgccatgtt cgaccagacc ggctccagcg cgcggcccag
    10381 cctggtgcgc cggaagaggt tgtacgcctc ctggagctgg tcgctggtct cggcgtgcca
    10441 ccagatcatg aggtcggcgt cggcgcgcag accggagacg tcgtaggtgc cgcggatcgt
    10501 cacgtccttg gcggcgagct ggtcgaacag ctcctggacc tcgtccgcgt atcccgcgcg
    10561 gtcctcgggc agcacgtcct tcagcttgaa gaccgaccag agggtgtagc ggatgacctc
    10621 gttgaggtcc ttggccagct tgcccttgtt cgggatcctg ccggactcgg tggtggaggc
    10681 gtcgtcactc atggtcccta ttgtcccgcg ccgccgtgca ggctctgcac cgggttggcc
    10741 gtcagctcgc ggaccgcgcg caggtcgccg tcgagctggt ccaccgccgc gttcgcgctc
    10801 gcgatgcagg cggggatgcc gacgccgtcg tactgcgcgc cgcacaccgc gagggccggc
    10861 agcgcggcca cgtgcgcgcg gatgcgggcc acgcgcgcgt ggtgcccgac ggggtactgg
    10921 ggaaggccgt cggtccagcg ggtgacgcgc gtcgccacgg gcaccgcgtc caggccggtg
    10981 gcctcgcgca ggtcgtgccg ggagatctcc acgaggccgg cgtcgtcccg cccgaggacc
    11041 gccgtctcgc cgtggcggcc gacggaggtg cggaccacga ccacgtcggg gtcctcctcg
    11101 gcgatccacc cccacttctg ggaggcgaag gtcgacgcct tgatggtgcg gccgtcgacc
    11161 ggcggcacca ggaagccgct gccctcgggc agccgcaggc cggcgcgccg gtaggcgagg
    11221 gtgaccaggg ccatcgaggc gtactcgacg gcggcgagtt cggcggcggc cgccggggac
    11281 tcggcgcgca gcaggccggc ggcggcgggg gcggggacgg cgaggacgac ggcgtccgcg
    11341 tgcagttccc ggtcgccgg
//
