LOCUS       NZ_LIWA01000005        10972 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB01883 scaffold5, whole genome shotgun sequence.
ACCESSION   NZ_LIWA01000005
VERSION     NZ_LIWA01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB01883
  ORGANISM  Streptomyces sp. CB01883
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 770318)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770318)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905855.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:34:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,089
            CDSs (total)                      :: 8,009
            Genes (coding)                    :: 7,788
            CDSs (with protein)               :: 7,788
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 85 of 221
            Pseudo Genes (incomplete)         :: 169 of 221
            Pseudo Genes (internal stop)      :: 25 of 221
            Pseudo Genes (multiple problems)  :: 50 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:33:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 593345
            Orig. end                         :: 604317
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIWA01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10972
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="4"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10972
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10972
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5972](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5972)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(1255..2283)
                     /locus_tag="AMK32_RS25455"
                     /old_locus_tag="AMK32_25010"
     CDS             complement(1255..2283)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /locus_tag="AMK32_RS25455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25010"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_073896356.1"
                     /transl_table=11
                     /translation="MSVLDEWTDRIAPEGTDTDPRNPDPTGDEVLRAMSDARSVDDFDM
                     RHLRYFLTVVRAGTVSAAAQELRISQPSLSQQIRRLEQRVGAALFIRSSRGVELTSGGR
                     AFLREIQGIPGQLRSAIAAAAPAPQAWPVGVCGGVPAEVLIEVQNALSGSRPDGSRETG
                     GKLRMRSVDAADQGELLRHGEIAFGIVRLPIPMPHVVRAVVRDEPLGLVMAREHPLAAH
                     PELTWQDLSRQRLLWYEEGCAPGFAETVPAHLAQLGWNSVLHPVNQDQHALFLHALQTT
                     ADLVALRPHHAVEREPHLVWRPLPTSRPPRERLAVITGSDCRTARLLRKVAAERGWAFV
                     AD"
     misc_feature    2386..2401
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.21"
                     /tool="antismash"
     misc_feature    complement(2387..2400)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.89"
                     /tool="antismash"
     gene            complement(2399..3046)
                     /locus_tag="AMK32_RS25460"
     CDS             complement(2399..3046)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="AMK32_RS25460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_206745526.1"
                     /transl_table=11
                     /translation="MTKGTDMTQQERARRTRERVLTAAAEVFASQGYSRATLSSVADRI
                     GMTKGALYGHFSSKRSLAGALIDESRQAWTSLRTEYDTPGADAGGVLEEVVVGFAGRLQ
                     SDVRLRAGVRLAADCPILARALSDILVEVHGALMELVRRAQRDSGFPAYSPRLVAHLVM
                     IVMYGLLHAPPGRVEEDRAAGGESLWRLLFNALSGAGGPGPLRAEDDAVSGP"
     gene            complement(3113..3814)
                     /locus_tag="AMK32_RS25465"
                     /old_locus_tag="AMK32_25020"
     CDS             complement(3113..3814)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /locus_tag="AMK32_RS25465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25020"
                     /product="HAD-IB family hydrolase"
                     /protein_id="WP_073896361.1"
                     /transl_table=11
                     /translation="MTTELRTACPALAFFDVDETVIAEKSMLDFWRHWSRLHPARVPPD
                     GPEWWAEAAVPPDRETLNRRYYRRYAGVPLADLEAAGRLWYDGYRRGGAAFVRSALRAV
                     ATHRAAGREVVLVSGSMRPLLAPLADELEVSTVVCTELVVGADGVLTGEVHRPMIGAAK
                     GEAVVRVMRERGADPRDCFAYGDHESDLAMLRAVGNPVVVGDSPVLNDEAERFGWSVAS
                     ARRGPFGSEGT"
     gene            complement(3811..4956)
                     /locus_tag="AMK32_RS25470"
                     /old_locus_tag="AMK32_25025"
     CDS             complement(3811..4956)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1191: acyl-CoA dehydrogenase type 2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035385.1"
                     /locus_tag="AMK32_RS25470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25025"
                     /product="oxidoreductase"
                     /protein_id="WP_241841126.1"
                     /transl_table=11
                     /translation="MTGGEGASDVRGVRSGRAGRPARDLLVEVARQDAQAADERRRLGP
                     QVAAALVDAGFPRHFVPVRWGGTAGTFTQLLADASAVAEACAAAGWCAALYAAHGRLAA
                     YLPERGQQDLWADGPDVRIAASVVPPQGRATAVTGGWRLDGEWRTASGVDHAEWVLLAS
                     WTPGADGAREHRVFAVPREELSVLDTWVSVGLRGSGSNSVSADGIVVPAHRSFTLGALL
                     SPLPDAARCHRVPYPMVAGLMFAAPVLGAARGALRAWADDCVQEGSRRPGQNLLLARAS
                     ARLHAAGLLLEGAADRADRQGTTPLAVAENRRDAATAAAWCREAVDDLFHASGLRGQSP
                     DSLLQRAWRDVTTAAAHGALGLDAAADAYADAALTRAGGVR"
     gene            complement(5001..5972)
                     /locus_tag="AMK32_RS25475"
                     /old_locus_tag="AMK32_25030"
     CDS             complement(5001..5972)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="AMK32_RS25475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25030"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_073896367.1"
                     /sec_met_domain="AfsA (E-value: 1.1e-19, bitscore: 65.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIGRPGPERHWQRLGFDRSVPRETVHKTAAAEVLLTDAERLGDE
                     RFAVGASWRRDHYLAHHGGPAADPVLLAETARQTAIYLSHRFFGVAHGVPFVLSGISVE
                     LFEALPPVDEEYLAVGLDVVCRKPAGGPRLRLELDADVVVRHRSVGRARVRWEPMEPRR
                     YALLRRRGGQGPGEAGPRTAPSCVPLPPARVGQLAERDVLLAADASAAHVWWLRLDRAQ
                     PVLFDHDSDHVPGMTLVEAFRQAAGVASADGRPGASGATLLDVTFTAFCEPDVPVSITA
                     ERAGRGAGVFTLRAHQAGRELASAEVGHGVPESSAVRLEAAC"
     gene            6175..6822
                     /locus_tag="AMK32_RS25480"
                     /old_locus_tag="AMK32_25035"
     CDS             6175..6822
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135623.1"
                     /locus_tag="AMK32_RS25480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25035"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_107423752.1"
                     /transl_table=11
                     /translation="MPEPHVNAFAPRRTPTRRGPDPKQERSVLTRLRVLRGAAELFTER
                     GYRQTSVKDVADRVEMTKGAVYFHYPTKEALALAIVDEHYARWPKILDEVNKEGLGPVD
                     TAARMLERAAVAFQDDVIVQAGARLQLERPQIDAELPTPYVDWISLLESLLVSAREAGE
                     LRPGVDPGPAARSLVSAFFGSQHVSDVLSGRADVVRRWRDLGDLLFRAIRSD"
     gene            complement(6927..8192)
                     /locus_tag="AMK32_RS25485"
                     /old_locus_tag="AMK32_25040"
     CDS             complement(6927..8192)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387370.1"
                     /locus_tag="AMK32_RS25485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25040"
                     /product="MFS transporter"
                     /protein_id="WP_073896373.1"
                     /transl_table=11
                     /translation="MSETRTTSVQAIDGGRRTGTAEAVAGRPAAPLWWVWLAAWPVTAV
                     FVLSNAATPLYVLWQRDIGFSKGTLTVVFAFYIVGLLGSLLVSGVVSDRVGRKPVLLPA
                     LTLGLAACLIFATATSVAALIVARLFTGIAVGAVVSAGMAAVTDVAGPERKRIAALLAS
                     CAMVFGAGLGPLLAGLLSELAPAPTVTVFVVEAVLLVTAVLCVLRMPVRRPATRAKGSW
                     VRVPGVPRGNGRQLALGIAVFAPGITATSFVLSLGPSLLSGLLDTTNRIVAGAMAFVMF
                     LAATGVQFAVQGLRRRTILTAGAVSTTLSMIMLIIAVHTSAVAVLIISALLAGAGQGMG
                     QLGGLSLLNSSVPTQRLAEANAALNVGGYIPAGILPVSAGYLSDAVGLTSGATIFGAVL
                     MGLAVIGGLVVRATRRQVTDPA"
     gene            8304..8681
                     /locus_tag="AMK32_RS25490"
                     /old_locus_tag="AMK32_25045"
     CDS             8304..8681
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387395.1"
                     /locus_tag="AMK32_RS25490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25045"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_073896376.1"
                     /transl_table=11
                     /translation="MPATRAAAPAIKVLPEPAELPAPLPEPGVGELRLETVLGALSDPL
                     RLTIVRKLLLESEDFDHSCGWFGFDRPKSSLTHHFKALREAGVTRQRQYGLERRSHVRI
                     DDLNARFPGLLDLVAAWTPQA"
     gene            complement(8778..9326)
                     /locus_tag="AMK32_RS25495"
                     /old_locus_tag="AMK32_25050"
     CDS             complement(8778..9326)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007388710.1"
                     /locus_tag="AMK32_RS25495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25050"
                     /product="hypothetical protein"
                     /protein_id="WP_073896379.1"
                     /transl_table=11
                     /translation="MGEGRTHLGPLELTDGHWGVGDATRPGTHWVEFRPDGLHQHEPDS
                     EGRSVPWSRIMTGIGITWGKHSWNTSSRGIYTLRGAVASRDGGWMHMTLRHPYENDLLR
                     FDQHVRPYRALDALRLEYLLRQLIDEGKPHLLGDPEWVGRAVAHLAGGKERWTTYRALR
                     QVAAEALAAAGPGGAAAGA"
     gene            9463..9816
                     /locus_tag="AMK32_RS25500"
                     /old_locus_tag="AMK32_25055"
     CDS             9463..9816
                     /EC_number="1.14.-.-"
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433719.1"
                     /locus_tag="AMK32_RS25500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25055"
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="WP_073896382.1"
                     /transl_table=11
                     /translation="MTHHPEAPVAPVEAYEPPYYAAVFTTVRTQEQSGYSETNARMEDL
                     VKDIPGFLGADHAQTPGGLGITVAYFRDADALTEWRTNAEHRAAQKRGQAEWYETYTLH
                     VARVERSHGFKRA"
     gene            complement(10042..10875)
                     /locus_tag="AMK32_RS25505"
                     /old_locus_tag="AMK32_25060"
     CDS             complement(10042..10875)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574917.1"
                     /locus_tag="AMK32_RS25505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_25060"
                     /product="PIG-L family deacetylase"
                     /protein_id="WP_073896385.1"
                     /sec_met_domain="PIG-L (E-value: 1.8e-29, bitscore: 98.6,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDRPLTLMAVHAHPDDEATGTGGVLARYAAEGIRTVLVTCTDGG
                     CGDGPGGVKPGDPGHDPAAVASMRRQELEESCAILKISHLETLDYADSGMMGWATNDAP
                     GSFWRTPVEEGAARLAELMRRYRPDVVVTYDENGFYGHPDHIQANRITMAALAMTEPTP
                     KVYWTTAPRSKMQQFGETMREFGGDWQEPDPDEAAALAEIGLPDEEITTWVDTAAFGGQ
                     KFDALAAHASQGENIFFLRMGKERFTELMGVETFVRVQDSTGAAVPENDLFAGLR"
     misc_feature    10931..10945
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
ORIGIN
        1 gcgctgagtg gtgtcgtccc gggccaccgc ggcggtccac tcgggctcga ccgtggcccg
       61 ctgagcggcg agttccgcgt cacggccggt ccaggtgcgg gtgaactcgt tgcccacgga
      121 gcgggcctgg taggcctcgt cccacgggat gccgcgcacc acgtcgaacg aacgggtgtc
      181 gacggtgtcc gcggtgcgcg cggagaccag gtgggacttg aacccggcgg tggccagaga
      241 ctcctcggtg gcggcgaacc gggtgcccat catcaccccg tcggcgccga gcgcgagggc
      301 cgcggccaga ccgcggccgt ccgcgaaccc gccggccgcg accaccggca ccgcgccctg
      361 cgtgacgtcg agtacggcgg ggatgagggg cagggtcgcc agcgacgcgt ggtggccgcc
      421 ggcctcggcg ccctgggcca cgagcacgga cgcaccggcc cgtacggcga cggcggcctg
      481 cccggcgtcg tgcacctgga cgacgacgtg ggacccggca tcggcggcgg acgccacgta
      541 cttctggacc tgtgccgtgt cgccgaagga gagggcgatg accggcgggg cgtaaccgag
      601 caccttgtcc cacagaccgg gtcgggtgtc gaaggtgaac atgacgcagc cgacgcccca
      661 gacgccaccg gtctccgagg cccgcgccac gtgttcgtcg atgaacgccg ggtcgccgta
      721 actgacgccg acgaggccga ggccgcccgc ccgggtgacg gcgccggcga gccggccacc
      781 ggacacggag cccatcgggg cgcagacgac cgggtggtcg atgcccagca gttcggtcag
      841 cttggtcgag atcacgatgc tccttggtcg ggtcgatggc atggcacggc ccgtcagggt
      901 gaagaggaag agatacgcca gggaattccg ctggggattt cccggcgaag gaggggcgag
      961 gcgttatccg gatggatgcc gtttcggagg gaaggccgcc cttgtttcgt tcaagaggct
     1021 atcttcggtc tccgcgggcc gtccaagacg caaacagcgt gacgtccata tgcgggcggc
     1081 tataccgcct gacggcataa acgggaaagg cgtgtgaccc ggccgtgaag gcctgggtca
     1141 cacgccggac gggcacgtgt gcggaactgg ggcctgtcgt ttggatcatg cccgcgtcgc
     1201 ggggcggcgg acaaggcacc gtgggccagg ccggcctgat ccggacgaca ggccctagtc
     1261 ggcgacgaac gcccagccgc gctccgccgc gaccttccgc aggagacggg cggtacggca
     1321 gtcactgccg gtgatgacgg cgagacgctc gcgcggcggc ctgctggtgg gcaggggccg
     1381 ccacaccagg tgcggctccc gctccacggc gtggtggggg cgcagggcga cgaggtccgc
     1441 cgtggtctgc aacgcgtgca ggaagagggc gtgctggtcc tgattcaccg ggtgcagcac
     1501 ggagttccac cccagctgcg ccagatgggc cgggacggtc tccgcgaacc ccggtgcgca
     1561 gccttcctcg taccagagca ggcgctggcg cgagaggtcc tgccatgtga gctccgggtg
     1621 tgcggcgagt ggatgttccc gcgccatgac gagaccgagg ggctcgtcgc ggaccacggc
     1681 gcgcacgacg tgcggcatgg ggatcggcag gcgcacgatg ccgaaggcga tctcgccgtg
     1741 ccgcagcagt tcaccctggt ccgccgcgtc caccgagcgc atccgcagct tcccgccggt
     1801 ctcgcggctg ccgtccggac ggctcccgga cagcgcgttc tgcacctcga tgagaacctc
     1861 cgccgggact ccgccgcaca caccgacggg ccaggcctgc ggtgcggggg ctgccgccgc
     1921 gatggcggaa cgcagttgac cgggaattcc ttgaatttcc cggagaaatg cccggccccc
     1981 gctcgtcaac tccacccctc gtgaactgcg gatgaacaat gcggcgccga cacgttgttc
     2041 aaggcgtcga atctgctgac tgagactcgg ctgcgatatc cggagttcct gggcggcggc
     2101 cgacacggtt ccggcgcgaa ccacggtcag gaagtagcga aggtggcgca tgtcgaagtc
     2161 gtcgacgctg cgcgcgtcgg acatcgcgcg cagtacctcg tcccccgtcg gatccgggtt
     2221 ccgcgggtcc gtgtccgtac cttccggggc gatccggtcg gtccactcgt ccagcacgct
     2281 catccgcttc cctcccagga gtcgccgccc ctgccccgcg cagggatgca cccacccggt
     2341 cccgccggtg cgggacccgg tgaagcatgg caaaagaatt cgttctcgaa ggtatgtttt
     2401 acggccctga aacagcgtcg tcctccgcgc gcaagggtcc tggcccgccg gctcccgaca
     2461 acgcgttgaa gagcagccgc cacaacgact ccccgccggc cgcgcggtcc tcctcgacgc
     2521 gtccgggggg cgcatgcagc agtccgtaca tcacgatcat caccagatgg gcgacgagcc
     2581 gcggcgagta cgcgggaaag ccgctgtccc gctgggccct gcgcaccagt tccatcaggg
     2641 cgccgtgcac ctcgacgagg atgtcggaca acgccctcgc caggatgggg cagtcggcgg
     2701 cgagccgaac cccggcccgc agtcgcacat ccgactgcaa gcggccggcg aaccccacga
     2761 cgacctcctc gagcaccccg ccggcatcgg cgcccggggt gtcgtactcg gtccgcaggc
     2821 tcgtccacgc ctgccgggac tcgtcgatca gggccccggc caacgatctc ttggaggaga
     2881 agtgtccgta caacgcaccc ttggtcatgc cgatgcggtc ggccaccgaa ctgagggtgg
     2941 ccctgctgta cccctgggag gcgaacacct ccgcagcggc cgtcagcact ctctccctcg
     3001 tgcgtcgcgc ccgttcctgt tgggtcatgt ctgtgccctt cgtcatggaa agagagcaca
     3061 tacataccat tggagcgaca cgtcaccgca gcgttccggc ccgccggaca tctcacgtgc
     3121 cctccgatcc gaacggcccc cggcgagcgg aggccaccga ccagccgaag cgctccgcct
     3181 cgtcgttcag cacgggagaa tctcccacca cgaccggatt ccccaccgcg cgcagcatgg
     3241 ccagatcgct ctcgtggtcg ccgtacgcga aacagtcccg cgggtccgca ccacgctccc
     3301 gcatgacccg gaccacggcc tcgcccttcg ccgccccgat catcgggcga tgcacctccc
     3361 cggtcagcac cccgtcggct cccaccacca actcggtgca cacgaccgtc gacacctcca
     3421 actcgtcggc caatggggcg aggagcggac gcatcgagcc cgagaccagc acgacctccc
     3481 ggccggcggc ccggtgcgtg gcgacggcgc gaagggcgga ccgcacgaag gccgcaccac
     3541 cgcgccgata tccgtcgtac cagagccggc ccgcggcttc gaggtcggcc aggggcacac
     3601 ccgcgtagcg ccggtagtag cggcggttca acgtctcacg atcgggaggc accgccgcct
     3661 ccgcccacca ctccggcccg tcggggggga ctcgcgccgg atgcagccgc gaccagtgcc
     3721 tccagaagtc gagcatgctc ttctcggcga tcaccgtttc gtccacgtcg aagaaggcca
     3781 gtgcgggaca cgcggtccgg agctccgtcg tcatcggaca cctcctgcgc gcgtcagagc
     3841 cgcgtccgcg tacgcgtcgg ctgccgcgtc gagcccgagg gcaccgtgcg ccgcggccgt
     3901 cgtgacatcc cgccacgcac gttgcagcag gctgtccggc gactgcccgc gcagtcccga
     3961 ggcgtggaag aggtcgtcga ccgcctcgcg gcaccatgcg gccgcggtcg ccgcgtcccg
     4021 gcggttctcc gccaccgcga gcggggtcgt cccctgccgg tccgcccggt ccgcggcacc
     4081 ctcgagaagc agacccgcgg cgtgcagccg tgcggacgcc cgggccagca gcaggttctg
     4141 gcccggtcta cgggacccct cctgtacgca gtcgtccgcc caggcccgca gcgccccacg
     4201 ggcggctccc aggaccggag ccgcgaacat cagcccggcg accatcggat agggaacccg
     4261 gtggcagcgg gccgcgtcgg gcaggggact caacagcgcg cccagcgtga aggaacggtg
     4321 cgcgggcacg acgatcccgt ccgccgacac gctgttgctt ccgctgcctc gcagtccgac
     4381 ggagacccag gtgtccagga cggacagctc ctcgcgcggc acggcgaata cccggtgctc
     4441 ccgtgcaccg tccgcgcccg gcgtccagga cgccagcaga acccattccg cgtggtcgac
     4501 accgctcgcc gtccgccact ccccgtcgag ccgccatccg ccggtcacag ccgtggcgcg
     4561 gccctgcggc ggaacgacgg aggcggcgat gcggacgtcg ggcccgtcgg cccagaggtc
     4621 ctgctgtccg cgctccggga ggtaggcggc tagacggccg tgcgccgcgt agagcgcggc
     4681 gcaccagccc gctgcggcgc aggcctccgc gaccgccgag gcatccgcga gcagctgcgt
     4741 gaaggtgccg gccgtgccgc cccatctcac cggtacgaag tggcgcggga accccgcgtc
     4801 cacgagggcg gcggccacct gcgggccgag cctgcgccgc tcgtcggccg cctgcgcgtc
     4861 ctgccgggcg acttcgacga gcaggtctcg ggccggccgc ccggcccggc cgctccgcac
     4921 accccggacg tccgacgcac cctcaccacc tgtcacatcg ccgtcatttc gttgtacacc
     4981 ggcggcattc tggcgggccg tcagcatgcc gcctccagcc gcactgcgga cgactccggc
     5041 acgccgtggc ccacttcggc ggaagcgagt tcacgcccgg cctgatgggc gcggagggtg
     5101 aagacgcccg cacctcggcc ggcccgttcc gccgtgatcg acaccggcac atccggctcg
     5161 cagaacgcgg tgaacgtcac gtcgagcagc gtcgcgccgc tcgcgccggg ccgaccgtcc
     5221 gccgaggcga ccccggcggc ctgccggaac gcctccacga gcgtcatgcc cggcacgtgg
     5281 tcggagtcgt ggtcgaacag cacgggttgt gcgcggtcga gccgaagcca ccagacatga
     5341 gccgctgatg cgtcggctgc gaggagcacg tcccgctcgg cgagctggcc gacgcgagcc
     5401 ggcggaaggg gcacgcagga cggtgccgta cgtggccccg cctctcccgg cccctgccct
     5461 ccgcgccgac gcaacagggc atagcggcgg ggctccatcg gctcccagcg aacccgtgcc
     5521 cgcccgacgg agcggtgccg caccacgacg tccgcgtcca gttccaggcg cagtcgcggg
     5581 cccccggcgg gcttgcggca gacgacgtcc agtccgacgg ccaagtactc ctcatcgacc
     5641 ggcggaagtg cttcgaacag ttcgacggag atcccgctca gcacgaacgg caccccgtgc
     5701 gccacaccga agaagcggtg cgacagatag atcgcggtct gccgagcggt ctcggccagc
     5761 agaaccggat cggcggcggg gccgccgtga tgagcgagat agtggtcccg gcgccatgag
     5821 gccccgaccg cgaaccgctc gtcaccgagc cgttccgcgt ccgtcagcaa cacctcggcc
     5881 gcggccgtct tgtggacggt ctcccgcggg acagagcggt cgaatcccag cctttgccag
     5941 tggcgctccg gccccggtcg gccgatgctc atccctgatc cagacatggc gtgcacgcgc
     6001 acaaacctcc ccctggccac cgtacggatc gcgccggtat cgtcccgacg cgcacggcga
     6061 ctcccgaaga aatatacctt caggaaggta tattgcaggg attattttgc gccaccgggc
     6121 acggccgagc gtgaccgcac acagcaggac cgagacctac agaggatgcc cctcatgcca
     6181 gagccgcacg tcaacgcctt cgccccgaga agaactccga ccaggagggg gcccgacccc
     6241 aaacaggagc gctcggtcct cacccgcctc cgggtcctgc gaggagccgc ggaactcttc
     6301 accgagcgcg ggtaccgcca gacgtcggtc aaggacgtgg ccgaccgggt cgagatgacc
     6361 aagggggccg tgtacttcca ctacccgacc aaggaggcac tggcgctggc catcgtggac
     6421 gagcactacg ctcgttggcc gaaaatcctt gatgaggtca acaaggaggg actcggcccc
     6481 gtggataccg cggcccgcat gctggagcgt gccgcggtcg cgttccagga cgacgtgatc
     6541 gtgcaggccg gcgcccggct gcagctcgaa cgcccgcaga tcgacgccga attgcccact
     6601 ccctacgtcg actggatttc gctcctggag tcgctgctcg tgtccgcccg ggaggcgggc
     6661 gaactccgtc ccggcgtcga tccgggcccg gccgcgcgct ccctggtctc ggcgttcttc
     6721 ggttcccagc acgtgtccga cgtcctgagc ggccgcgcgg acgtggtgcg gcgctggcgc
     6781 gacctgggcg atctgctctt ccgggcgatc cggtccgact gagcacgcga cggcccgacc
     6841 atgcacagca cgctcccggg ggttggtgca ccaccgccgt ccagcggtgc accacgcccc
     6901 gggagcgtgc cgttcctccg ggcccgtcag gcggggtccg tgacctgacg cctggtggct
     6961 cggaccacca ggccgccgat gacggcgaga cccatgagca cggcaccgaa gatcgtggcg
     7021 ccactggtca ggcccacggc gtcactgagg tagcccgcgg acacgggcag gatgccggcc
     7081 gggatgtaac cgcccacgtt gagcgcggcg ttggcctcgg caagacgctg cgtgggcaca
     7141 ctcgagttga gcagcgacag tccgccgagc tggcccatgc cctggccggc gccggcgagc
     7201 aacgcggaga tgatgaggac ggcgaccgcc gaggtgtgca cggcgatgat cagcatgatc
     7261 atgctcagcg tggtgctgac cgcacccgcc gtgaggatgg tgcggcggcg cagcccctgg
     7321 accgcgaact gcactccggt ggcggcgagg aacatcacga acgccatggc accggcgacg
     7381 atccggttcg tggtgtccag caggccggac agcagcgagg ggcccagcga gagcacgaag
     7441 gacgtggcgg tgatgcccgg cgcgaacacc gcgataccga gagcgagttg acggccgttg
     7501 ccacgcggta cgcccggtac ccgcacccag ctgcccttgg cgcgcgtcgc gggacggcgc
     7561 accggcatcc gcagcacgca gagcaccgcc gtgaccagta gcacggcctc gacgacgaag
     7621 acggtgacgg tcggcgcggg cgccagctcg gagagcagcc cggccagcag cgggccgagc
     7681 ccggcaccga acaccatggc gcaggaggcg aggagtgcgg cgatccgctt gcgctcgggt
     7741 ccggcgacgt cggtcaccgc ggccatgccg gccgagacga cggcgccgac cgcgatcccg
     7801 gtgaacagcc gcgccacgat cagcgcggcc acgctggtgg cggtcgcgaa gatcaggcag
     7861 gcggccagcc cgagcgtcaa cgccgggagc agcacgggct tgcggccgac ccggtcggaa
     7921 accaccccgg acaccagcag cgagccgagc agccccacga tgtagaaggc gaacaccacg
     7981 gtcagcgttc ccttggagaa gccgatgtcg cgctgccaga gcacgtacag cggcgtcgcc
     8041 gcgttcgaga gtacgaacac ggccgtgacc ggccatgcgg ccagccacac ccaccacagc
     8101 ggtgccgcgg gcctgccggc caccgcctcg gcggtgccgg tacgtcgccc cccatcgatg
     8161 gcctgcacgg atgtggtccg ggtctcggac atgactgctc ctcccgatcg ctcctgccgc
     8221 atctcagcag tacgagttga gtcgaactta gcatgcagta cgatgagact cgtacatagg
     8281 aatcgcgtta tgaggagttg cctatgcctg cgaccagggc cgccgcaccc gccatcaagg
     8341 tgctccccga gccggccgaa ctgcccgcgc cactgccaga gcccggtgtc ggcgagctgc
     8401 gtctggagac cgttctcggc gcgctcagcg atcctctgcg cctgacgatc gtccgcaaac
     8461 tcctcctgga gtccgaggac ttcgaccact cctgcggctg gttcgggttc gaccggccca
     8521 agtcgtcgct cacccaccac ttcaaagcgc tgcgcgaggc gggagtcacg cgtcagcgcc
     8581 agtacggcct cgaacgccgc agccatgtgc gcatcgacga cctcaacgcc cggttccccg
     8641 gccttctcga cctggtggcg gcctggactc cgcaggcttg acgcctgcca ggcaatgccg
     8701 agcgaccttc ccgggtgcgc ggaccgcacg gtgtgtgcgc gtgcggggcc gccggccccg
     8761 tgccgcggcg aaagccgtca ggcgcccgcg gcggcaccgc ccgggccggc cgccgccagc
     8821 gcttccgccg ccacctgccg caacgcccgg tacgtggtcc agcgttcttt cccgccggcc
     8881 aggtgggcca cggcacggcc cacccattcc gggtcaccga ggaggtgcgg ctttccctcg
     8941 tcgatgagct gccgcagcag gtactccagc cgtagggcgt ccagtgcccg gtacgggcgc
     9001 acatgctggt cgaagcgcag caggtcgttc tcgtacgggt gacgcagcgt catgtgcatc
     9061 cagccgccgt cgcgggaggc gaccgccccc ctcaacgtgt agatgcccct gctgcttgtg
     9121 ttccaggagt gcttgcccca ggtgatcccg atgccggtca tgatcctgga ccaggggacg
     9181 gaccgtccct cggagtccgg ctcgtgctgg tgcagcccgt ccgggcggaa ctccacccag
     9241 tgggtgcccg ggcgcgtggc gtccccgact ccccagtggc cgtcggtcag ttccagcggt
     9301 cccagatgtg tacggccttc ccccatacgg tcattgaacc atgtgcccta tgccccgttc
     9361 ttccggctgt gccacggtct ttggtcaaga gctggatccc gtacgtatcc cgttgcggaa
     9421 agacgctgga gagcggggct cggaccgccc tacggtgacg ccatgaccca tcacccggaa
     9481 gcacctgtag cgcccgtcga ggcgtacgaa cccccttact acgcggcggt cttcaccacg
     9541 gtgcggaccc aggagcagag cggctacagc gagaccaacg cccgcatgga ggacctggtg
     9601 aaggacattc cggggttcct gggcgcggat cacgcgcaga cccccggcgg gctcggcatc
     9661 accgtcgcgt acttccggga cgccgacgcc ctcacggagt ggcggaccaa tgccgagcac
     9721 cgggcggcgc agaagcgcgg gcaggccgag tggtacgaga cctacacgct tcatgtggcg
     9781 agagtggagc gtagccacgg gttcaagcgc gcctagggtc tgtcgtttgg atcaggccgg
     9841 tctggcccac ggtgccttgt cggccgaccc gagcggggcg tggtgcgtgc agctgcaagg
     9901 cggaggaggg cggcgacgcg gagcgtcgac aaccgacgac aacgccgcag atgcgcgtgc
     9961 cacgccccgc gacgccggcg tgatccaaac gacaggccct aggtggtgtg cgccttccgg
    10021 gccctccgga aggcgggcgg atcagcgcag gccggcgaag aggtcgttct cgggtacggc
    10081 cgcgccggtg gagtcctgta cgcgcacgaa ggtctcgacg cccatcagct cggtgaacct
    10141 ttccttgccc atcctgagga agaagatgtt ctcgccctgg ctggcgtgcg cggccagggc
    10201 gtcgaacttc tggccgccga aggccgcggt gtccacccag gtggtgatct cctcgtccgg
    10261 gagtccgatc tcggccagcg cggcggcctc gtcgggatcc ggctcctgcc agtccccacc
    10321 gaactcacgc atggtctcac cgaactgctg catcttcgag cgcggcgccg tcgtccagta
    10381 caccttcggc gtcggctcgg tcatcgccag cgccgccatg gtgatgcggt tcgcctggat
    10441 gtggtcgggg tgaccgtaga agccgttctc gtcgtaggtg acgaccacat cgggccggta
    10501 gcggcgcatg agttccgcga gtcgtgcggc accctcttcc acaggcgtcc gccagaagga
    10561 accgggggcg tcattggtcg cccagcccat catcccggag tctgcgtagt ccagcgtctc
    10621 caggtgactg atcttcagga tggcgcagct ctcctcgagt tcctgacggc gcatcgaggc
    10681 gacggctgcc gggtcgtgcc cggggtcgcc cggcttcaca ccccccggtc cgtctccgca
    10741 cccgccgtcg gtacacgtca cgagtaccgt gcggatgcct tccgccgcgt aacgtgcgag
    10801 gacgcctcct gttcccgtgg cctcgtcgtc ggggtgggcg tgcacggcca tgagcgtcaa
    10861 gggccggtcg ttcatgaaac agtcctcctg tgggaagggg ccgccgccgg agtccagggg
    10921 cgggcgcaag aacagtcccg tcgattatcg ggtgaccacg gtgtcggccg gg
//
