LOCUS       NZ_LIWA01000003        10405 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB01883 scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_LIWA01000003
VERSION     NZ_LIWA01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB01883
  ORGANISM  Streptomyces sp. CB01883
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1077901)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077901)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905855.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:34:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,089
            CDSs (total)                      :: 8,009
            Genes (coding)                    :: 7,788
            CDSs (with protein)               :: 7,788
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 85 of 221
            Pseudo Genes (incomplete)         :: 169 of 221
            Pseudo Genes (internal stop)      :: 25 of 221
            Pseudo Genes (multiple problems)  :: 50 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:33:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 88613
            Orig. end                         :: 99018
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIWA01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            952..2046
                     /locus_tag="AMK32_RS14600"
                     /old_locus_tag="AMK32_14370"
     CDS             952..2046
                     /NRPS_PKS="Domain: Aminotran_5 (74-315). E-value: 4.5e-28.
                     Score: 90.1. Matches aSDomain:
                     nrpspksdomains_AMK32_RS14600_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121393.1"
                     /locus_tag="AMK32_RS14600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_14370"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_073894065.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.6e-22, bitscore:
                     75.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLSAERFAAIEDGVARLLDTRRDVLITQGEALLPLE
                     GAIRAAAGPGTVALNVITGPYGQTFGNWLRDSGATVHDISVPFHTAVTPGQIQQAFADH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRRHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAISVSERAWARMAENPHAPRRSYLSLLDWKDRWIDAGRRVLPHAP
                     AQLEMLALQACLERIGSAGLDTVMARHRGAALATRAGAVALGGGLEPYVYEAADAAPVA
                     TTLRVPSDLVASELVARALESDPALPLAAGGGALAKEMIRVNHYGPDATLDTVRRSLTA
                     LGAALAEAGAPVDIESAARALNTP"
     aSDomain        1174..1896
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK32_RS14600_Aminotran_5.1"
                     /evalue="4.50E-28"
                     /label="AMK32_RS14600_Aminotran_5.1"
                     /locus_tag="AMK32_RS14600"
                     /protein_end="315"
                     /protein_start="74"
                     /score="90.1"
                     /tool="antismash"
                     /translation="RDSGATVHDISVPFHTAVTPGQIQQAFADHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEVVRRHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     ISVSERAWARMAENPHAPRRSYLSLLDWKDRWIDAGRRVLPHAPAQLEMLALQACLERI
                     GSAGLDTVMARHRGAALATRAGAVALGGGLEPYVYEAADAAPVATTLRVPSDLVASELV
                     ARALESDPALPLAAGGGAL"
     misc_feature    2921..2936
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.78"
                     /tool="antismash"
     misc_feature    2923..2937
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.79"
                     /tool="antismash"
     misc_feature    2950..2964
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.24"
                     /tool="antismash"
     misc_feature    3043..3058
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3177..3695
                     /gene="ectA"
                     /locus_tag="AMK32_RS14605"
                     /old_locus_tag="AMK32_14375"
     CDS             3177..3695
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671932.1"
                     /locus_tag="AMK32_RS14605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_14375"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_073892417.1"
                     /transl_table=11
                     /translation="MTAAPADLLIDRPAMTDGATLWRLAKDSKTLDLNSSYSYLLWCRD
                     FAGTSAVARGADGEPVGFITGYVRPDRPRTLLVWQVAVDSAHRGHGIAAALLDGLTARL
                     TAERGITEVETTITPGNTASERLFTSFAARHGAQVDREVLFGADLFPDGPHDPEVLYRI
                     GPLTASTPH"
     gene            3709..4980
                     /gene="ectB"
                     /locus_tag="AMK32_RS14610"
                     /old_locus_tag="AMK32_14380"
     CDS             3709..4980
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.8e-73.
                     Score: 239.4. Matches aSDomain:
                     nrpspksdomains_AMK32_RS14610_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="AMK32_RS14610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_14380"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_073892420.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.8e-78, bitscore:
                     259.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRSWPAVFDRAQGSRMYDEDGHAY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLSRDGVTHGLDMSTTAKRSFLQTFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALKELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFRPELDVWEPGEHNGTFRGNNPAFVTATAALETYWTDGSAMEKQTRTRGEQVEQAL
                     ISITEENLADVKEYRGRGLVWGLEFRDKDRASRVARRAFELGLLIETSGPEGEVVKLLP
                     ALTITPDELDEGLSILARSVRETV"
     aSDomain        3793..4806
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK32_RS14610_Aminotran_3.1"
                     /evalue="1.80E-73"
                     /label="AMK32_RS14610_Aminotran_3.1"
                     /locus_tag="AMK32_RS14610"
                     /protein_end="366"
                     /protein_start="28"
                     /score="239.4"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHAYLDFFAGAGSLNYGHNNPVLKRALIDYLS
                     RDGVTHGLDMSTTAKRSFLQTFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALKELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFRPELDVWEPGEHNGTFRGNNPAFV
                     TATAALETYWTDGSAMEKQTRTRGEQVEQALISITEENLADVKEYRGRGLVWGLEFR"
     gene            5001..5405
                     /locus_tag="AMK32_RS14615"
                     /old_locus_tag="AMK32_14385"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="AMK32_RS14615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_14385"
                     /product="ectoine synthase"
                     /protein_id="WP_073892423.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.7e-54, bitscore:
                     178.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSVHETILY
                     AGTETSMWYANHIEAVVCTKGEAELTDHEAGKTYTITPGTMYLLDGHERHTLRVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5411..6313
                     /gene="thpD"
                     /locus_tag="AMK32_RS14620"
                     /old_locus_tag="AMK32_14390"
     CDS             5411..6313
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671935.1"
                     /locus_tag="AMK32_RS14620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_14390"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_073892425.1"
                     /sec_met_domain="PhyH (E-value: 2.5e-40, bitscore: 134.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVTDLYPTRGTSEVTVPRQDPVVWGSPDTPGPIPTADLQAYER
                     DGFLAVDQLITPDEVEVYRRELERLVTDPAIRADERSIVEPKSKEIRSVFEVHKISELF
                     ANLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSIALTENFATNGGLMIMPGSHRTFLGCAGATPEDNYKKSLQMQDAGTPSDEALTEL
                     AGEHGIRLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFSAPVR
                     RPDFIGARDFTPVGQTPVK"
     gene            complement(6390..7451)
                     /locus_tag="AMK32_RS14625"
                     /old_locus_tag="AMK32_14395"
     CDS             complement(6390..7451)
                     /NRPS_PKS="Domain: Aminotran_5 (20-347). E-value: 2e-25.
                     Score: 81.4. Matches aSDomain:
                     nrpspksdomains_AMK32_RS14625_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671936.1"
                     /locus_tag="AMK32_RS14625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_14395"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_073894067.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.9e-20, bitscore:
                     67.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METTETFERIVRAEFTPKSTYLNTASNSLLPARTVAALQEAALLR
                     AEGRPLTPLYEDVEACRAAYARLAGVPAGRVALGASVAAHTGVIAASLPAGAEVLTADD
                     DFTSVLNPFHVRGDLLVRTVPLERIAESVRPGTALVAVSAAQSADGRIADLPALRAAAR
                     AHGARTYVDFSQAAGWLPMDAGAYDFTASVSFKWLMGPHGTAFLTVPEDFGGLRPVLAG
                     WTAGERPWESCYGPVDELALSARRFDLTHALFTLAGLRRSLDLVEELGVSEIHAHDVAL
                     ADRFRTGLSDLGHKPVPAPGSAIVSVPGLGSRQPELSEAGIEISDRAGNLRASFHLYNT
                     TADVDRLLDALSG"
     aSDomain        complement(6411..7391)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK32_RS14625_Aminotran_5.1"
                     /evalue="2.00E-25"
                     /label="AMK32_RS14625_Aminotran_5.1"
                     /locus_tag="AMK32_RS14625"
                     /protein_end="347"
                     /protein_start="20"
                     /score="81.4"
                     /tool="antismash"
                     /translation="YLNTASNSLLPARTVAALQEAALLRAEGRPLTPLYEDVEACRAAY
                     ARLAGVPAGRVALGASVAAHTGVIAASLPAGAEVLTADDDFTSVLNPFHVRGDLLVRTV
                     PLERIAESVRPGTALVAVSAAQSADGRIADLPALRAAARAHGARTYVDFSQAAGWLPMD
                     AGAYDFTASVSFKWLMGPHGTAFLTVPEDFGGLRPVLAGWTAGERPWESCYGPVDELAL
                     SARRFDLTHALFTLAGLRRSLDLVEELGVSEIHAHDVALADRFRTGLSDLGHKPVPAPG
                     SAIVSVPGLGSRQPELSEAGIEISDRAGNLRASFHLYNTTADVDRL"
     gene            complement(7547..8269)
                     /locus_tag="AMK32_RS14630"
                     /old_locus_tag="AMK32_14400"
     CDS             complement(7547..8269)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381151.1"
                     /locus_tag="AMK32_RS14630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_14400"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_073892428.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDEVEVVHRSFELDP
                     GRAKGDVQPVITMLTRKYGMSEAQAQAGEDNLGAQAAAEGLDYRTRDRDHGNTFDMHRL
                     LHLAKEHGRQDQLIQAFYRANFAEERSVFTEGDERLVELAVEAGLDADRVRAVLADPDA
                     YADAVRADEREAAQLGANGVPFFVLDRTYGVSGAQPAEVFTQALSQAWGERSPLRLIDE
                     GDDAQACGPDGCAVPQHG"
     gene            8425..9387
                     /locus_tag="AMK32_RS14635"
                     /old_locus_tag="AMK32_14405"
     CDS             8425..9387
                     /NRPS_PKS="Domain: PKS_KR (31-113). E-value: 4e-07. Score:
                     22.1. Matches aSDomain:
                     nrpspksdomains_AMK32_RS14635_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430790.1"
                     /locus_tag="AMK32_RS14635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_14405"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_073892431.1"
                     /transl_table=11
                     /translation="MSTAQHKIGSGFGATSTADDVLRGVDLTGKLALVTGGYSGIGLET
                     TRALVKAGARVVVPARRPATAEQALAGLSGVELDELDLGDLDSVRGFAERFLASGRTLD
                     IVIDSAGIMACPETRVGPRWEAQFATNHLGHFALVNRLWPAIAPGGARIVSVSSRGHHF
                     SGIRWEDVHWRRGYDKWEAYGQAKTANALFAVHLDRLGRDSGVRAFSLHPGGIITPLQR
                     HLPKAEMVERGWIDEDGNPLAPEGFKTPPQGAATQVWAATSPQLDGLGGVYLEDCDIAE
                     PATEGEERTGVKAWATDPEQAARLWALSAELTGVDAFAA"
     aSDomain        8518..8763
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK32_RS14635_PKS_KR.1"
                     /evalue="4.00E-07"
                     /label="AMK32_RS14635_PKS_KR.1"
                     /locus_tag="AMK32_RS14635"
                     /protein_end="113"
                     /protein_start="31"
                     /score="22.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="ALVTGGYSGIGLETTRALVKAGARVVVPARRPATAEQALAGLSGV
                     ELDELDLGDLDSVRGFAERFLASGRTLDIVIDSAGIM"
     CDS_motif       8521..8598
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AMK32_RS14635_0001"
                     /evalue="8.50E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="AMK32_RS14635"
                     /protein_end="58"
                     /protein_start="32"
                     /score="24.8"
                     /tool="antismash"
                     /translation="LVTGGYSGIGLETTRALVKAGARVVV"
     gene            complement(9403..10011)
                     /locus_tag="AMK32_RS14640"
                     /old_locus_tag="AMK32_14410"
     CDS             complement(9403..10011)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="AMK32_RS14640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK32_14410"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_073892433.1"
                     /transl_table=11
                     /translation="MDVELPELPFPLRTYGPDGHWFHEDGVLSGWAGPRQDRFVTPTGE
                     ALDPASDAPRLLGAPEGDFQLIARVTVGFRAAFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFTVDGSSAWLRVSRTGRSFAFHASRDGRRWTFVRLFTLGDEKE
                     TGAALVGFMTQSPLGEGCVVTYDHIEFRPAWPSDLRDGS"
ORIGIN
        1 gatcgccttg ttgatggcgt cgcgcagggc cgtgttgccc ttcttcacgg tgaagccgta
       61 ctgctcaccg gtcttcaggt tgtcgacgac cttgaacttg tccgcgttcg ccttgtcctt
      121 gagccagccc tggacgaccg ggtagtcgat gacgacggcc tggacctggc cggtacgcag
      181 gccgttgagg accgcgtcgg aggactcgaa ggagatcggg tcgtagccct tgctcttgac
      241 gtagtcctcg ccggtggtct gcgcctgggc gcccagcttc ttgcccttgg ccttgacgtc
      301 ggcgagcgag ttcacaccgc tgctcttgtc gaccaggacg gcctgggtgg cctcgaagta
      361 cgggtcggag aagtcgacgt tcttcttgcg ctcggcggtg atggtcatgc cggcggcggc
      421 caggtcgcac tgcccggagt tgaggaaggc gccggtcttg aagttctcga agggcgtgtc
      481 gaggatctgc tgcttgacgc ccaggtcctt ggcgacgagg tcgatgaggg acacgtcgaa
      541 gccctgcacc ttgccgtcga tctccgactg gaacggcggg tagggcaggt gggtgcaggt
      601 ggtcagctgc ccggccttgg ccaggtggac gcccttgacc gtggtcttgc cgctccccga
      661 gtcgctggag gagcagccgg ccacgacgag cacgagtgcg gcggtcgcgg tggtggcggc
      721 cagaaggcgg gtccggcgcc cgaggagcgt attcatgggg gatctcctat gggggaactg
      781 tggcttccga ttataaggag atgtttgggt cactcaaacc aaaccgatgg catcggagcc
      841 gtccggcctg agaagtcgcc ggttcgcggg cgcggatgtg gcgagggtta cgctcgtgtg
      901 cgtcggcctc cggccggccc gtgaaccctc gtgagcgaag agagcaccgc cgtgacccac
      961 ccgttcctcg atctgccccc gctgagtgcc gagcgcttcg ccgccatcga ggacggggtg
     1021 gcccggctgc tggacacccg gcgggacgtg ctgatcacgc agggcgaggc gctgctgccg
     1081 ctggagggcg cgatccgcgc ggcggccggt cccggcacgg tggccctgaa cgtgatcacg
     1141 ggcccgtacg ggcagacctt cggcaactgg ctccgggact ccggcgcgac cgtgcacgac
     1201 atctccgtgc ccttccacac ggcggtgacg cccgggcaga tccagcaggc gttcgccgat
     1261 cacccggaga tcgacttcgt gtcgctggtg cacgccgagg cggccaccgg caacaccaac
     1321 ccggtcgcgg agatcggcga ggtggtacgg cggcacggcg cgctgttcta cctggacgcg
     1381 gtcgcctccg tgggcgccga gccggtgctg ccggacgcgt ggggcgtgga cctgtgcgtg
     1441 atcggggcgc agaaggcgat gggcggcccg gcgggcgtgt cggcgatctc ggtgagcgag
     1501 cgggcgtggg cccggatggc ggagaacccg cacgcgccgc gccgctccta cctgtcgctg
     1561 ctcgactgga aggaccgctg gatcgacgcc ggccgcaggg tgctgccgca cgcccccgcc
     1621 cagctggaga tgctggccct tcaggcgtgc ctggagcgca tcgggtcggc ggggctggac
     1681 acggtgatgg cccggcaccg gggcgccgcg ctggccacgc gggcgggtgc ggtcgcgctc
     1741 ggcgggggcc tggagccgta cgtgtacgag gccgcggacg cggcgccggt cgccacgacg
     1801 ctccgggtgc cgtcggatct ggtggcgtcg gagctggtgg cgcgggccct ggagagcgac
     1861 ccggcgctgc cgctggccgc gggcggcggc gccctggcca aggagatgat ccgggtcaac
     1921 cactacggcc ccgacgccac cctggacacg gtccgccgat ccctcaccgc gctgggcgcg
     1981 gcgctggccg aggcgggagc accggtggac atcgagtcgg ccgcccgagc cctgaacacc
     2041 ccctgaccga ccacaccgcc ccggcaggga ggctcaccca gccacccccg agcgggcacc
     2101 gacccccggc cgacgccacc ggcaagacga ccggccaccc accgacgcga tgcggcaccg
     2161 ccgcaggcca caggcgggcg ctcgccagcc gcaggcgggg gggcacctgg acgcggacac
     2221 ggccgcccgg gcacaccggg ccaagggctc cggcaccccc gagcgggaca ccggcccccg
     2281 gccgacgccg ccggcaagac gacccgccgc caactggcgc gcacggcacc gccgccggtc
     2341 gcgggcgagc gttcgccagc tacgggcgga tgcgcacgga cacagatacg gccgcccgcc
     2401 ccacggccac cccggcaggg gccggcccca gtccgcgggg atgcagacac ggccacccgc
     2461 ccccgggctc accgggccaa gggctccgac acccccgagc gggacaccgg cccccggccg
     2521 acgccgccag caagacgacc ggccgcccac gggcgcgcac ggcaccgacg ccggtcgcgg
     2581 gcaggcgttc gccagctacg ggcggatgcg cacgaacaca gatacggccg cccgccccac
     2641 tgccaccccg gcaggggccg gccaccagtc cgcggggatg cagacacggc cgcccgcccc
     2701 cgcgctcacc gggccgagag ccccggcacc cctgagcgga ccgccggccc cggccgacgt
     2761 cggcaagacg accggccgcc cgggctcacc ggggcgaagt tcggcggcgc cgtggggcgt
     2821 cggtgggtgg taggtgttcg gcgggcggag gcgggggttt ggccggggca gcgggcgtgc
     2881 gcattttgtg tgagcgcgtc ggcgaaacca gcggatcgtg aattcaattc aatttcacaa
     2941 actccgccaa ttcttcggac gatagcttcc cgtcttcttc tgcccgcttt ccgcggagct
     3001 aaacggggaa gttttccggc acgtttcgga cggtttcctt gttgtgacac actccacaac
     3061 cagacacctt tgtcccgatt ttccgtgggg taatacgggc atttcccccg accccctgcg
     3121 cggcgcagcg gaaccgcccg tgcaatttct aattcgcctc gctaaattcg tgccgcatga
     3181 ctgccgcacc cgcagacctt ctcatcgacc gaccggcgat gaccgacgga gccacgctct
     3241 ggcgtctcgc caaggactcg aaaaccctcg acctgaactc ttcgtacagc tatctgctgt
     3301 ggtgccggga cttcgccggc acctcggcgg tggcgcgcgg tgcggacgga gagccggtcg
     3361 gcttcatcac cggctatgtg cgaccggacc gtccccggac gctgctcgtc tggcaggtgg
     3421 ccgtcgactc cgcccaccgg ggccacggca tcgccgccgc cctgctcgac gggctgaccg
     3481 cacggctcac cgccgagcgc ggcatcacgg aggtggagac cacgatcacc cccggcaaca
     3541 ccgcctccga gcgcctgttc acctcgttcg ccgcccggca cggcgcacag gtcgaccgcg
     3601 aggtgctgtt cggcgccgac ctcttcccgg acggcccgca cgaccccgaa gtcctgtacc
     3661 gcataggccc gctgaccgcc tccacccccc actgaggagc gaatcaccgt gaccatcacc
     3721 cagcccgacc tcagcgtgtt cgagaccctc gagtccgagg tccgcagcta ctgccgcagc
     3781 tggcccgccg tcttcgaccg tgcccagggc agccgcatgt acgacgagga cggccacgcg
     3841 tacctcgact tcttcgccgg cgccggctca ctcaactacg gccacaacaa cccggtactg
     3901 aaacgggcgt tgatcgacta tctgtcccgg gacggggtca cccacggcct ggacatgtcg
     3961 accacggcca agcggtcgtt cctgcagacc ttccaggacc tggtgctgcg cccgcgcgac
     4021 ctgccgtaca aggtcatgtt cccgggcccg acgggcacca acgccgtcga gtcggcgctg
     4081 aagctggccc gcaaggtgaa gggccgggag gcgatcgtgt cgttcacgaa cgccttccac
     4141 gggatgtccc tcggctccct cgccgtcacc ggcaacgcct tcaagcgggc cggcgccggc
     4201 atccccctgg tgcacggcac ccccatgccg ttcgacaact acttcgacgg ccaggtcccg
     4261 gacttcctgt ggttcgagcg gctgctggag gaccagggct ccggcctgaa caagcccgcc
     4321 gccgtgatcg tggagaccgt gcagggcgag ggcggcatca acgtcgcccg gcccgagtgg
     4381 ctgcgcgcgc tgaaggagct gtgcgagcgg caggacatgc tgctgatcgt cgacgacatc
     4441 cagatgggct gcggccgcac gggcgccttc ttctccttcg aggaggcggg gatcaccccc
     4501 gacatcgtca cggtgtccaa gtcgatcagc ggctacggcc tgcccatgtc gctgtgcctg
     4561 ttccggcccg agctggacgt ctgggagccc ggcgagcaca acggcacctt ccgcggcaac
     4621 aacccggcgt tcgtcacggc caccgcggcc ctggagacgt actggaccga cggctcggcc
     4681 atggagaagc agacccgcac ccgcggcgag caggtcgaac aggcgctgat ctccatcacc
     4741 gaggagaacc tcgccgatgt gaaggagtac cgcggacggg gactcgtgtg gggtctcgaa
     4801 ttccgtgaca aggaccgggc ttcgcgggtc gcccgccgcg ccttcgagct cgggctgctc
     4861 atcgagacgt ccggcccgga gggcgaggtc gtcaaactgc tgccggccct caccatcacc
     4921 cccgacgagc tggacgaggg gctgagcatc ctcgcccgtt cggtccgcga aaccgtctag
     4981 caaggaggaa gtacagcacc gtgatcgtcc gatcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcacgtcaa ggcgaagtcc ggcacgtggg agagcaagcg catcgtcctc gccaaggagc
     5101 gggtcggctt ctccgtgcac gagaccatcc tgtacgccgg taccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtcgtgtgca ccaagggtga ggccgaactc accgaccacg
     5221 aggccgggaa gacgtacacg atcacgcccg gcaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct gcgcgtcaag gaggacttcc actgcatctg cgtgttcaac ccgccggtca
     5341 ccgggcggga ggaccacgac gagaacggcg tgtacccgct gctcaccgag cccgaggagg
     5401 tgtgacaccc atgaccactg tcaccgacct gtaccccacc cgcggcacca gcgaggtgac
     5461 cgtcccccgg caggacccgg tcgtctgggg ctccccggac acgccgggcc cgatcccgac
     5521 cgccgatctc caggcgtacg agcgggacgg cttcctcgcc gtcgaccagc tgatcacccc
     5581 ggacgaggtc gaggtgtacc ggcgcgagct ggagcgcctg gtcaccgacc cggcgatccg
     5641 cgccgacgag cgctcgatcg tcgagccgaa gtccaaggag atccgctcgg tcttcgaggt
     5701 gcacaagatc agcgagctgt tcgcgaacct ggtgcgcgat ccccgcgtgg tcggccgggc
     5761 gcggcagatc ctcggctcgg acgtgtacgt ccaccagtcc cggatcaacg tcaagccggg
     5821 cttcggcgcg agcggcttct actggcactc ggacttcgag acctggcacg ccgaggacgg
     5881 cctgccgaac atgcgcacgg tgtccgtctc gatcgcgctg accgagaact tcgccaccaa
     5941 cggcgggctc atgatcatgc cgggctcgca ccggaccttc ctgggctgcg ccggcgccac
     6001 gcccgaggac aactacaaga agtcgctgca gatgcaggac gcgggcacac cgtccgacga
     6061 ggcgctgacc gagctggcgg gcgagcacgg catccgcctg ttcaccggca aggccggctc
     6121 ggcgacctgg ttcgactgca actgcatgca cggctccggc gacaacatca cgccgttccc
     6181 gcgcagcaac gtcttcatcg tgttcaacag tgtggagaac acggcggtgg agccgttctc
     6241 ggctccggtg cgcaggccgg acttcatcgg cgcgcgcgac ttcaccccgg tcgggcagac
     6301 cccggtgaag tgaccccccg agcaccgggc cggggcctcc tcgtgtggga cgggaggccc
     6361 cggcccggtc gtgtccggga ccggccggat cagccggaca gggcgtccag caggcggtcc
     6421 acgtccgcgg tcgtgttgta gaggtggaag gacgcgcgca ggttgcccgc gcggtcggag
     6481 atctcgatgc ccgcctcgct caactccggc tgccgggagc cgagtccggg caccgagacg
     6541 atcgccgagc cgggggcggg cacgggcttg tgcccgaggt ccgagagccc ggtccggaag
     6601 cggtcggcga gggccacgtc atgggcgtgg atctcggaca ccccgagttc ctcgacgagg
     6661 tccagggagc ggcgcagccc cgcgagggtg aacagggcgt gggtgaggtc gaaccgccgc
     6721 gcggaaagcg cgagttcgtc gaccgggccg tagcagctct cccagggccg ctcgcccgcc
     6781 gtccagcccg cgagcaccgg cctcagcccg ccgaagtcct cgggaacggt gaggaaggcc
     6841 gtcccgtgcg ggcccatcag ccatttgaag gagacggagg cggtgaagtc gtacgccccc
     6901 gcgtccatcg gcagccagcc cgccgcctgg gagaagtcga cgtaggtgcg tgcgccgtgc
     6961 gcgcgggcgg ccgcgcgcag ggcgggcagg tcggcgatgc ggccgtcggc ggactgggcg
     7021 gcgctgaccg cgacgagcgc ggtgcccggc cggacggact cggcgatccg ctccagcggg
     7081 accgtgcgga ccaggaggtc gccccggacg tggaacgggt tgagcacgga ggtgaagtcg
     7141 tcgtcggcgg tgaggacttc ggcgcccgcg ggcagcgagg ccgcgatcac cccggtgtgc
     7201 gcggcgacgg atgcgcccag cgcgacccgg ccggccggta cgccggccag ccgggcgtac
     7261 gcggcccggc aggcctcgac gtcctcgtac agcggggtca gcggccggcc ctcggcccgc
     7321 agcagcgccg cctcctggag ggcggccacg gtacgggcgg ggagcaggct gttgctggcg
     7381 gtgttgaggt aggtgctctt cggggtgaac tcggcgcgga cgattctctc gaacgtctcc
     7441 gtggtctcca tgggaccact ctgcggtccg gcgaagtccc cgtccattgc gaaattttgc
     7501 ggggatccgc ttagggatcc ttatacatgc cctccgatct gcggtttcag ccgtgctgcg
     7561 ggaccgcgca gccgtccggg ccgcacgcct gggcgtcgtc accctcgtcg atcagccgca
     7621 gcggggagcg ctcgccccag gcctggctca gcgcctgggt gaagacctcg gccggctgcg
     7681 cgccggagac gccgtaggtc cggtcgagga cgaagaaggg cacgccgttg gcgccgagct
     7741 gggcggcctc gcgctcgtcg gcgcggacgg cgtcggcgta cgcgtcgggg tcggcgagca
     7801 ccgcgcggac gcggtcggcg tccagaccgg cctcgacggc gagctccacc aggcgctcgt
     7861 cgccctccgt gaagaccgac cgctcctcgg cgaagttggc ccggtagaag gcctggatca
     7921 gctggtcctg cctgccgtgc tccttggcga ggtgcagcag gcggtgcatg tcgaaggtgt
     7981 tgccgtggtc gcggtcccgg gtgcggtagt ccagcccctc ggcggcggcc tgcgcgccca
     8041 ggttgtcctc gccggcctgg gcctgcgcct cgctcatgcc gtacttcctg gtcagcatgg
     8101 tgatcaccgg ctggacgtcg cccttggcgc ggcccgggtc cagctcgaag gagcggtgca
     8161 ccacctcgac ctcgtcgcgg tgcgggaagg cggccagcgc cttctcgaag cgtgccttgc
     8221 ccacgtagca ccaggggcag gcgatgtcgc tccagatctc gacgcgcatt cttcggctct
     8281 ctcacggtcg tacgggcatg gaggctctct ccgcccggtg cgtgaacctt caaacagcgc
     8341 gcttcattcc ccggccccgc ctcacccgtc cggcccgggg ctccgcttga cttcgagagc
     8401 actccaattc gtagcgttcc gggcatgagc actgcacagc acaagatcgg ttccggtttc
     8461 ggcgccacca gcaccgccga cgacgtcctg cggggcgtcg acctgaccgg gaagctcgcc
     8521 ctcgtcaccg gcggctactc gggcatcggc ctggagacca cccgcgcgct cgtgaaggcc
     8581 ggcgcgcgcg tggtcgtgcc cgcccgtcgt ccggccaccg ccgagcaggc gctggccggt
     8641 ctgtccggcg tcgagctcga cgagctggac ctcggcgacc tggacagcgt gcgcggcttc
     8701 gccgagcggt tcctcgcctc cggacggacc ctcgacatcg tgatcgacag cgccgggatc
     8761 atggcgtgcc cggagacccg ggtggggccc cgctgggagg cccagttcgc caccaaccac
     8821 ctcggtcact tcgccctcgt caaccggttg tggccggcga tcgcccccgg cggcgcccgg
     8881 atcgtctccg tgtcctcgcg cggccaccac ttctccggca tccgctggga ggacgtgcac
     8941 tggcggcggg gttacgacaa gtgggaggcc tacggccagg ccaagaccgc caacgccctg
     9001 ttcgccgtcc atctcgaccg gctcggccgg gactccggtg tgcgcgcgtt ctccctgcac
     9061 cccggcggca tcatcacccc gctgcagcgg cacctgccca aggcggagat ggtcgagcgc
     9121 ggctggatcg acgaggacgg caacccgctc gcccccgagg gcttcaagac cccgccgcag
     9181 ggcgcggcca cccaggtgtg ggcggccacc tctccccagc tggacggtct cggcggcgtc
     9241 tacctggagg actgcgacat cgcggaaccc gccaccgagg gcgaggagcg caccggcgtc
     9301 aaggcctggg cgaccgaccc ggagcaggcg gcacggctgt gggcgctgtc ggcggaactg
     9361 acgggcgtcg acgcgttcgc cgcgtagcgg acggcagggg cctcagctgc cgtctctcag
     9421 gtcgctcggc caggcgggac ggaactcgat gtggtcgtag gtgaccacgc agccctcccc
     9481 cagcggggac tgggtcatga agccgaccag cgcggcgccg gtctccttct cgtcgcccag
     9541 ggtgaagagc cggacgaagg tccagcgccg gccgtcgcgg gaggcgtgga aggcgaacga
     9601 ccggccggtc cggctgaccc gcaaccacgc cgagctgccg tccacggtga aggagttggc
     9661 gtcgtcggag tgcccgcggg tgaccaccgt gcagacggtg ggcacgtccg gagagtactc
     9721 caggcacagc ttggcccagg cccgttcgcc gacgtggacg tagaggaccc cggcgtcgaa
     9781 cgcggcgcgg aatcccacgg tgacccgggc gatcagctgg aagtcgccct ccggcgcgcc
     9841 cagcagccgg ggcgcgtcgg aggccgggtc gagggcttcg ccggtgggcg tgacgaaccg
     9901 gtcctgccgg gggcccgccc atccggacag cacaccgtcc tcgtggaacc agtgcccgtc
     9961 gggcccgtac gtgcggaggg ggaagggcag ttcgggaagt tccacgtcca tcgtccgatt
    10021 gtcgcagcag ctcgttcgcg ccgggccggg cccccgcggg cctaccgctc cagcaccccg
    10081 tcgaaccgcc ggggcagccc cagggggttg tcgtcgcgca gttccctcgg cagcagggcc
    10141 tgcggcgcgt tctggtagac caccggccgc agccaccgct cgatggccgt accccccacg
    10201 gaggtggacc cggaggtcgt cgcggggtac ggaccgccgt ggtgctgggc cggggccacg
    10261 gccacgccgg tgggccacgc gttcacgacc acgcgcccgg ccagcggggt cagctcggcg
    10321 aggatctccg ggccccgccc ctgtccggcg gcctcctcgg aggagagctg gacggtcgcg
    10381 gtgaggttgc ccggcagccg ggaca
//
