LOCUS       NZ_LIVZ01000014        10882 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0107 scaffold14, whole genome shotgun sequence.
ACCESSION   NZ_LIVZ01000014
VERSION     NZ_LIVZ01000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0107
  ORGANISM  Streptomyces sp. TSRI0107
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 324662)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324662)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905845.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,745
            CDSs (total)                      :: 7,667
            Genes (coding)                    :: 7,467
            CDSs (with protein)               :: 7,467
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 85 of 200
            Pseudo Genes (incomplete)         :: 158 of 200
            Pseudo Genes (internal stop)      :: 13 of 200
            Pseudo Genes (multiple problems)  :: 51 of 200
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:19:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 113797
            Orig. end                         :: 124679
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVZ01000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10882
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10882
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10882
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5882](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5882
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(409..419)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            527..1723
                     /locus_tag="AMK31_RS32625"
                     /old_locus_tag="AMK31_32085"
     CDS             527..1723
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514080.1"
                     /locus_tag="AMK31_RS32625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32085"
                     /product="Ig-like domain-containing protein"
                     /protein_id="WP_073944491.1"
                     /transl_table=11
                     /translation="MARTVRARALVRTAVAAALLAPLAACSADSAPAAPDAGKGGKDGD
                     RPVAVTVTPTGRQAAAGERVKVTAAGGRLTSVTVTDAEGRRLAGAVARDGRTWISDRKA
                     VPGTAYEVTAATRTRGGTAGTTRASFTTSPADKVNKVDWRPGTGTTVGIAQPISLVFDN
                     PVENRAEVEKQLKVTTSNGTEGSWGWIRDWSGRDRVDWRPETYWKPGTKVTLNAELNGT
                     DSGSGGWFVRDYTSTFTIGARQVVEVDLDSHRLTLVRDGRTVRRIPVSGGTPGGDKRSW
                     RGTAVLMAKEGTINMNSETVGLGDAYDKMVDYSMRLTWSGMYAHAAPWNARYFGNANRS
                     SGCIGMSDADAAWFYDQVRPGDPFEITGKDTKGVVEPGNGFGAWNLSWAEWQRRSALR"
     gene            complement(1769..2542)
                     /gene="cobF"
                     /locus_tag="AMK31_RS32630"
                     /old_locus_tag="AMK31_32090"
     CDS             complement(1769..2542)
                     /EC_number="2.1.1.152"
                     /GO_function="GO:0043819 - precorrin-6A synthase
                     (deacetylating) activity [Evidence IEA]"
                     /GO_process="GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cobF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135372.1"
                     /locus_tag="AMK31_RS32630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32090"
                     /product="precorrin-6A synthase (deacetylating)"
                     /protein_id="WP_073944333.1"
                     /sec_met_domain="TP_methylase (E-value: 8.3e-30, bitscore:
                     98.4, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKIHVIGIGAGDPDQLTLQAVRALRGTDVFFLLEKGEVKSDLTG
                     LRRDMLKTHVPEGTYRIVEARDPERDRRADGAAYSPAVGDWRGARADIYERLILEELGE
                     DQTGAFLVWGDPSLYDSTLGILEEVLGRGAVAFEYDVVPGISSVSSLVARHRTGLNRVA
                     GAVQITTGRRLSEGFPEGVDDVVVMLDAHQTFREYADQDIHIYWGAYLGTPDEITVSGP
                     IAEAAPRIESLRAEARERKGWIMDTYLLRRNPGQG"
     gene            complement(2592..3170)
                     /locus_tag="AMK31_RS32635"
                     /old_locus_tag="AMK31_32095"
     CDS             complement(2592..3170)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019070849.1"
                     /locus_tag="AMK31_RS32635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32095"
                     /product="DUF309 domain-containing protein"
                     /protein_id="WP_073944334.1"
                     /transl_table=11
                     /translation="MGGKGRGSSTSEGRDAEGAAAVARDRDAEGRARNARPRDGLGRPL
                     PYGAPGVERQPEGVVRTPEETVAEAQALLDAGKPFHAHEVFEDAWKSGPDEERTLWRGL
                     AQLAVGLTHSARGNVTGGARLLRRGAGAVEAWAESSGRPDVYGMDLTGVVAWARELAGV
                     VEAGADGSDGVGGAAVVDARTRAPRLYGA"
     tRNA            3269..3342
                     /anticodon=(pos:117100..117102,aa:Pro,seq:ggg)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="AMK31_RS32640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="AMK31_32100"
                     /product="tRNA-Pro"
     gene            3269..3342
                     /locus_tag="AMK31_RS32640"
                     /old_locus_tag="AMK31_32100"
     misc_feature    3474..3491
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.29"
                     /tool="antismash"
     gene            complement(3508..4650)
                     /locus_tag="AMK31_RS32645"
                     /old_locus_tag="AMK31_32105"
     CDS             complement(3508..4650)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381900.1"
                     /locus_tag="AMK31_RS32645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32105"
                     /product="transposase"
                     /protein_id="WP_241842797.1"
                     /transl_table=11
                     /translation="MREHSVTTVRRPAATMPTALDAAACRRLFGSLHLNGQRVKAERYV
                     RGLLSVPGRKTLQSIAAQFDGASAQQSVHHFISTSPWEWMPIRHAAARHAQRVLAPEAW
                     VIGSTLIPKTGPHSVGTDPQPTPMGTVNGQQAVGTWLASQRSAVPVDWQLRLSARWTAD
                     PLRRRASVPENAAYGTVEECVQQAVHGLLHIAGMRRLPVVVDIPEVNGMAIARFLTTTG
                     LPFVVRVSPVTPVRIDRSRLPMYGDLLRAAGELAESLPYVRHPANPGDGRTTGVMIPVV
                     APPPRREPMTLLDERRPDRRAGRRLWLTNLDPSAVTAALRLTRLPGVVTRDCAAISDGV
                     GLRDFAGRSFPGWHRHITLASVAHLMAACEATEMNRTTVR"
     misc_feature    4798..4813
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 21.04"
                     /tool="antismash"
     misc_feature    4922..4937
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    4929..4942
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    complement(4930..4943)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.36"
                     /tool="antismash"
     gene            5001..5882
                     /locus_tag="AMK31_RS32650"
                     /old_locus_tag="AMK31_32110"
     CDS             5001..5882
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="AMK31_RS32650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32110"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_073944335.1"
                     /sec_met_domain="AfsA (E-value: 4.3e-16, bitscore: 53.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYDITLDRRPTAQAGLGAYAHLKRQEHLLVTGWKRLAAHDFTLRV
                     RWPAVRGDLPYDPRVLAQTIRQTGLVVAHGAYDVPLTHQTMLSTLDFTISPGLRIPRGR
                     TSALDVRVTVSEGGGRRRTTRALHMTFQVLMDGAVVARAESEFGWIPERVYTRVRGDRR
                     HVDWAAWPLPAPVDAASVGRVSAVDVMLGAGDRPDRWLLRNDPDNHLLFDHPVDHVPGL
                     ALLEAADQAARALRGPAPFTPGRIEAVYWRYVEFDQPCWIAAEPLPEPGAVRVTGTQEG
                     EPVFRVDFRAGE"
     gene            complement(5923..7308)
                     /locus_tag="AMK31_RS32655"
                     /old_locus_tag="AMK31_32115"
     CDS             complement(5923..7308)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321070.1"
                     /locus_tag="AMK31_RS32655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32115"
                     /product="MFS transporter"
                     /protein_id="WP_198948621.1"
                     /transl_table=11
                     /translation="MDGSVLYLAMPGITSAITPTTDQALWILDIYGFVVSSLLIAFGSL
                     GDRYGRLRFLLTGAVVFGIGSTGAAFSPNPETLIACRALMGLGGATLLPAGLAIIGGLF
                     PDPRQRSRAIGVFAATFAAGFAVGPVFGGLLLSRFGWGSVFLINIPVVVLFVAFAPVLL
                     REVRTTDPGRVDALSVVLSAVGILLAVHAVKSAAAEAFSATEAITGALGVALLVWFVRR
                     QLALEHPLLDLRLFKDRVFTVAILTGVLSLVAWAAAGYLTGVYLQSVLGHGVLTAALLV
                     LPGAAVLTAACVGASGVVERIGKRAALITSHFFMGGGLLLLLLTTTEHGVAAFVASSVV
                     TGVGYGFSFSLVADTAVAAVPAERAGSAASIAETSNELGNALGISLLGSLAAAVFRLTG
                     PDVAGTLDETLAEPGLTAATVARAEDAFVDGLHAAIGVASLLCLALGVLALRWVPREEP
                     ASR"
     gene            7599..8192
                     /locus_tag="AMK31_RS32660"
                     /old_locus_tag="AMK31_32120"
     CDS             7599..8192
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="AMK31_RS32660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32120"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_158074566.1"
                     /transl_table=11
                     /translation="MATAVRVADAEGVGALSMRRLANELNSGVMSLYRHVANKDALLTA
                     ITRAACDEHPYPEPAPADWREAMRLAARQDWKVYTAHPWALVTSSSARHFSDTSCLDWM
                     MDALADLTDDPDLARSFSIAVWSYVQGVCIQHVAQELLPAGDRPEQSPLRATEEDFETG
                     LETLLDGMEQRARHAGPALREPRSAPAFPAAPAQ"
     gene            8226..9134
                     /locus_tag="AMK31_RS32665"
                     /old_locus_tag="AMK31_32125"
     CDS             8226..9134
                     /NRPS_PKS="Domain: PKS_ER (18-300). E-value: 1.5e-37.
                     Score: 121.4. Matches aSDomain:
                     nrpspksdomains_AMK31_RS32665_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155344.1"
                     /locus_tag="AMK31_RS32665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32125"
                     /product="zinc-binding dehydrogenase"
                     /protein_id="WP_073944338.1"
                     /sec_met_domain="ADH_N (E-value: 1.8e-06, bitscore: 22.0,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.9e-19, bitscore:
                     64.2, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIAIRFDRFGGPEVLTPVEVAAPEPGPDELLVTVEAAGVNFADIH
                     QADGSYLDPGQLPHIPGSEVVGRTDDGRRVLAKVSHGYAEQAVAKASAVVDIPEDLDAG
                     AALALLVQGLTAWHLLRTAARLGTGETVVVHSAAGGVGSLAVQLAREFGAGRVWAQAST
                     PEKRRLALDLGADAAVDYPLPERAEVILDAVGGELFDHALDSLASGGRLVTYGNSARTG
                     FTPLDPARLSRLNAAVVGFWLRPTLAAPGAFAGPLKELLSLTAQGRLKPVVHPAYPLTE
                     ARRAHEDLLARRTTGKVVLHP"
     aSDomain        8280..9125
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK31_RS32665_PKS_ER.1"
                     /evalue="1.50E-37"
                     /label="AMK31_RS32665_PKS_ER.1"
                     /locus_tag="AMK31_RS32665"
                     /protein_end="300"
                     /protein_start="18"
                     /score="121.4"
                     /tool="antismash"
                     /translation="EVAAPEPGPDELLVTVEAAGVNFADIHQADGSYLDPGQLPHIPGS
                     EVVGRTDDGRRVLAKVSHGYAEQAVAKASAVVDIPEDLDAGAALALLVQGLTAWHLLRT
                     AARLGTGETVVVHSAAGGVGSLAVQLAREFGAGRVWAQASTPEKRRLALDLGADAAVDY
                     PLPERAEVILDAVGGELFDHALDSLASGGRLVTYGNSARTGFTPLDPARLSRLNAAVVG
                     FWLRPTLAAPGAFAGPLKELLSLTAQGRLKPVVHPAYPLTEARRAHEDLLARRTTGKVV
                     L"
     CDS_motif       8610..8672
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AMK31_RS32665_0001"
                     /evalue="1.80E-07"
                     /label="PKSI-ER_m5"
                     /locus_tag="AMK31_RS32665"
                     /protein_end="149"
                     /protein_start="128"
                     /score="23.6"
                     /tool="antismash"
                     /translation="GETVVVHSAAGGVGSLAVQLA"
     gene            9176..9940
                     /locus_tag="AMK31_RS32670"
                     /old_locus_tag="AMK31_32130"
     CDS             9176..9940
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (9-171). E-value: 1.1e-17. Score:
                     56.5. Matches aSDomain:
                     nrpspksdomains_AMK31_RS32670_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988472.1"
                     /locus_tag="AMK31_RS32670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32130"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_073944339.1"
                     /sec_met_domain="adh_short (E-value: 1.3e-38, bitscore:
                     126.9, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 6.2e-34, bitscore:
                     112.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSDLLAGKVVLVTGASSGIGAAAAKVFCQEGATVVLAARREDRL
                     AALTEELRAKGAEAAHVVCDVSVAEDAARAVEFAVAEYGRLDGAFNNAGVGGDRTPLHL
                     MSDDVYDTVMDVNVRGVWNCLRYEIAAMLKGGGGAVVNNSSVAGLVAIPAAAPYIAAKH
                     AVVGLTRAAADEYAQQGIRVNAVAPGTTRSEITEDWFRRNPGLEQTVNAMTPQGRTAEP
                     EEIAAAAAWLLSDRCPFLTGTVLPVDGGFVNQ"
     CDS_motif       9203..9298
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AMK31_RS32670_0001"
                     /evalue="7.40E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="AMK31_RS32670"
                     /protein_end="41"
                     /protein_start="9"
                     /score="12.2"
                     /tool="antismash"
                     /translation="VVLVTGASSGIGAAAAKVFCQEGATVVLAARR"
     aSDomain        9203..9688
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK31_RS32670_PKS_KR.1"
                     /evalue="1.10E-17"
                     /label="AMK31_RS32670_PKS_KR.1"
                     /locus_tag="AMK31_RS32670"
                     /protein_end="171"
                     /protein_start="9"
                     /score="56.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VVLVTGASSGIGAAAAKVFCQEGATVVLAARREDRLAALTEELRA
                     KGAEAAHVVCDVSVAEDAARAVEFAVAEYGRLDGAFNNAGVGGDRTPLHLMSDDVYDTV
                     MDVNVRGVWNCLRYEIAAMLKGGGGAVVNNSSVAGLVAIPAAAPYIAAKHAVVGLTRA"
     gene            10119..10757
                     /locus_tag="AMK31_RS32675"
                     /old_locus_tag="AMK31_32135"
     CDS             10119..10757
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047020.1"
                     /locus_tag="AMK31_RS32675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_32135"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_073944340.1"
                     /transl_table=11
                     /translation="MARQERAIRTRESILEAMASLFIEVGYEAATIAALVERTGLTRGA
                     LYFHFPTKEAMAHGVLARAVTREGILPQTYKLQEWVDLGLLLAHRLPREPMLRAAVQLS
                     VDPKARSLFGTRWPDWIDVGQDLLEEAKERGELQPHAVPAEIARLTVGAWTGVQLVTEA
                     LPDGLGLSEEIARLYQLILPNVASIGVLAKLDTSPYRGARLLEAEERSA"
ORIGIN
        1 cgggaaggta ggcgaagtac ttgcgggtgc cggggaactg ccgcagcagc accagggcca
       61 ccccggtcag ctcgccggcc tccggatccg gcccccagcc gagcagctgg gcgcgccagc
      121 cctccttgac gccggcccag gacgggcact gcaggaaacc ggcgccgagc gccgcgccgc
      181 gcggggaggc gaggaaggcg cggtaggtct cggcggtgac gggtccgagc ctgaggtccc
      241 ggttccgttc ctcgtgctgg cggccgggcg gtacgagcag cgctgacacg gtctgtgagc
      301 ctctccttct ccccggctgt cgcggggacg cagaggatgc tcacagccgc ccgtgaccgt
      361 cccgcgcggc gaatgtgacc ggacgacgac cgttccccac cagtcgccgt cacattgccc
      421 ggacgccgct gaccaggact tatccggctc tagagtcacg acatcttccg ccttgtccgt
      481 caccacggag gtcccgttgt cccgcacacc catacgcgtc cacgccctgg cccgaaccgt
      541 ccgcgcccgc gcccttgtcc gcaccgccgt cgccgcggcc ctgctggcgc cgctcgccgc
      601 ctgttccgcg gactccgctc ccgccgcgcc ggacgcgggc aagggcggga aggacggtga
      661 ccgtcccgtc gccgtcacgg tcaccccgac gggacggcag gccgcggccg gtgagcgggt
      721 gaaggtcacc gcggcgggtg gccgcctcac ctcggtgacc gtcaccgacg ccgagggccg
      781 gcggctcgcc ggcgcggtcg cccgtgacgg ccggacgtgg atctcggacc gcaaggccgt
      841 acccggcacc gcgtacgagg tgacggcggc gacccggacg cggggcggca ccgccggcac
      901 cacccgggcc tccttcacca cgtccccggc ggacaaggtc aacaaggtcg actggcgacc
      961 gggcaccggc accaccgtcg ggatcgccca gccgatctca ctggtcttcg acaaccccgt
     1021 cgagaaccgg gccgaggtcg agaagcagct gaaggtcacc acctcgaacg gcaccgaggg
     1081 gtcctggggc tggatacgcg actggtcggg ccgggaccgg gtggactggc ggcccgagac
     1141 gtactggaag cccgggacca aggtcacgct gaacgccgaa ctgaacggca ccgactcggg
     1201 cagcggcggc tggttcgtcc gcgactacac gagcaccttc accatcggcg cgcggcaggt
     1261 cgtcgaggtc gacctcgaca gccaccggct caccctcgtc cgcgacggca ggacggtccg
     1321 gcgcattccc gtctccggcg gcacgcccgg cggcgacaag cgctcctggc gcggtaccgc
     1381 cgtgctgatg gccaaggagg gcaccatcaa catgaactcc gagaccgtcg gcctcggcga
     1441 cgcctacgac aagatggtcg actactcgat gcggctgacc tggtcgggga tgtacgcgca
     1501 cgccgccccg tggaacgccc gctacttcgg caacgccaac cgcagttcgg gctgcatcgg
     1561 gatgagcgac gcggacgcgg cctggttcta cgaccaggtg cggcccggcg acccgttcga
     1621 gatcaccggc aaggacacca agggggtcgt cgagccgggg aacggcttcg gcgcgtggaa
     1681 cctgtcatgg gccgagtggc agcggcggag cgccctgcgc tgatcgcggc ggcgcccgca
     1741 gcccttcgcc ccttcgcgga cgtggtgcct agccctgtcc cgggttgcga cgcagaaggt
     1801 aggtgtccat gatccagccc ttgcgctccc gggcctcggc ccgcaggctc tcgatgcgcg
     1861 gcgcggcctc ggcgatcggg ccggagacgg tgatctcgtc cggggtgccg aggtaggcgc
     1921 cccagtagat gtggatgtcc tggtcggcgt actcgcggaa ggtctgatgg gcgtcgagca
     1981 tcacgacgac gtcgtcgacg ccctcgggga agccctcgga gagccgccgc ccggtggtga
     2041 tctgcacggc accggccacc cggttcagcc ccgtgcgatg ccgcgcgacc agcgacgaga
     2101 cgctgctgat gccgggcacc acgtcgtact cgaaggccac ggcgccccga cccagcacct
     2161 cctccaggat gcccagcgtg ctgtcgtaca gcgacggatc gccccacacc aggaacgcgc
     2221 cggtctggtc ctcgcccagc tcctcgagga tcagccgctc gtagatgtcg gcgcgggcgc
     2281 cgcgccagtc gccgacggcc ggcgagtagg ccgcgccgtc cgcgcgccgg tcccgctccg
     2341 gatcacgggc ctccacgatc cggtaggtgc cctccggtac gtgcgtcttc agcatgtccc
     2401 ggcgcagccc cgtgagatcg gacttcacct cgcccttttc gagcaggaag aacacatccg
     2461 tgccgcgcag cgcccggacc gcctggaggg tgagctggtc ggggtcgccc gcgccgatac
     2521 cgatgacatg aatctttcgc acgccccgag tctgccgtac ggcactgaca gcgccgccac
     2581 cgcccgtgcc ctcacgcccc gtacagccgg ggcgcgcggg tccgggcgtc caccaccgcg
     2641 gccccgccga ccccgtcgga gccgtccgcc ccggcctcca ccacgcccgc cagctcccgc
     2701 gcccacgcca ccacgccggt cagatccatc ccgtacacgt ccggccgccc ggacgactcc
     2761 gcccacgcct ccaccgcgcc cgccccgcgc cgcagcagcc gcgccccgcc ggtgacgttg
     2821 ccgcgggccg agtgggtgag ccccacggcg agctgggcga ggccccgcca cagcgtgcgc
     2881 tcctcgtcgg gccccgactt ccaggcgtcc tcgaacacct cgtgcgcgtg gaacggcttc
     2941 cccgcgtcca gcagcgcctg cgcctccgcc acggtctcct cgggcgtccg tacgaccccc
     3001 tccggctgcc gctccacgcc cggcgccccg tacggcagcg gccgcccgag cccgtcccgc
     3061 ggccgcgcgt tccgtgcccg cccctccgcg tcccggtccc gcgccacggc cgccgcgcct
     3121 tccgcgtccc ggccctccga cgtgctgctt ccgcgtccct ttccacccat acgacgattg
     3181 tgccgcccgg tgcccccttc ggcgccgccg ggcccgtggg gtaaagttct gtccgcgcga
     3241 tcacgcgggt caccgcggga ggacgcaccg ggacgtggcg cagcttggta gcgcacttga
     3301 ctgggggtca aggggtcgca ggttcaaatc ctgtcgtccc gacttttcga agtcgcaggc
     3361 cgaggggccg tttcagggca tctgaaacgg cccctcggcc gttttcgccg ttccccgcgt
     3421 cgcacgacgg cctcggaaat caggaagccg aatacatcgc acgttgttgc gcgcatccca
     3481 tgatcgggat aaggcggcct gatgatatta ccgaacggta gtcctgttca tctccgtggc
     3541 ctcgcaggcc gccatcagat gggcgacgga cgccagcgtg atgtgccggt gccagccggg
     3601 gaaggagcgg cccgcgaagt cgcgcaggcc gacgccgtcc gagatggccg cgcagtcgcg
     3661 ggtgacgacg ccgggcaggc gggtgagccg cagtgcggcg gtgacggcgg aggggtcgag
     3721 gttggtcagc cacaggcgcc ggccggcgcg gcggtcgggg cgccgctcgt cgaggagtgt
     3781 catcggctcc cgccgcggcg gcggcgccac cacggggatc atcacgccgg tggtccggcc
     3841 gtcaccgggg ttcgccgggt gccgcacgta cggcagcgac tcggccagtt cgccggcggc
     3901 gcgcagcaga tcgccgtaca tgggcagcct cgaccggtcg atgcgcaccg gtgtcaccgg
     3961 gctcacccgc accacgaacg gcagtcccgt cgtcgtcagg aagcgggcga tggccatgcc
     4021 attcacctcg gggatgtcca cgaccaccgg cagccgccgc atcccggcga tgtgcagcag
     4081 cccgtggacg gcctgctgca cgcactcctc gacggtgccg tacgcggcgt tctcgggaac
     4141 gctcgcccgc cgccggaggg ggtcggccgt ccagcgggcg gacagccgca gctgccagtc
     4201 gaccggtacg gccgaccgct gcgacgcgag ccaggtgccc accgcctgct gaccgttgac
     4261 ggtgcccatc ggggtgggct gcggatcggt gccgacggag tgcggcccgg tcttcgggat
     4321 cagcgtggag ccgatcaccc atgcctccgg ggccaggacg cgctgcgcgt gccgggccgc
     4381 ggcgtgccgg atcggcatcc actcccaggg cgaggtgctg atgaagtggt gcacgctctg
     4441 ctgcgcggac gccccgtcga actgcgccgc gatgctctgc agcgtcttgc ggccgggcac
     4501 cgacagcagc ccgcggacgt accgctcggc cttcacccgc tgcccgttga ggtgcaggga
     4561 accgaagagt ctgcggcagg cggctgcgtc cagggccgtc ggcatcgtcg ccgccggccg
     4621 tcggaccgtc gtgaccgaat gttcccgcac gttgcgcatc gtcgtccccc cgtgaccgca
     4681 aaaaaggact ctcgtcctgt tttttatacg agggtacgcc ggacgcagcc gtatgttttc
     4741 ggccaatgac gttcggaggg gcgctctctc cgcgccgggg cggtggcggg aagcggaact
     4801 ggcgtagtcc acaggcgagt cgggtccatc gggagggccg ttgatggtgg tggcctcgcg
     4861 tccgcgctcg gaggggtggg aagcgctctc tgccggaacg cgttgtgcgc acacgcccct
     4921 tcgcacgtat ttttggccaa aaccgaacgg agggccggta ctttaaacgg cactggagcc
     4981 tctccgactc agggggaacg ttgtacgaca tcactctcga ccgtcgcccg accgctcagg
     5041 ccggtctcgg cgcatacgcc catctcaagc ggcaggagca tctgctcgtc accggctgga
     5101 agcgcctggc cgcgcacgac ttcaccctgc gtgtcaggtg gcccgccgtg cggggggacc
     5161 tgccgtacga cccccgcgtc ctcgcgcaga cgatccggca gaccggactg gtcgtggcgc
     5221 acggcgcgta cgacgtcccg ttgacgcacc agaccatgct gagcaccctc gacttcacga
     5281 tcagccccgg gctgcgcata ccccgcgggc ggacgtcggc gctggacgtg cgggtcaccg
     5341 tgtccgaggg cgggggcaga aggcgtacca cgcgcgcgct gcacatgacc ttccaggtgc
     5401 tcatggacgg ggccgtggtg gcccgtgccg agagcgagtt cggctggatc ccggagcgcg
     5461 tgtacacgag ggtgcgcggt gaccggcgcc atgtcgactg ggcggcctgg ccgttgcctg
     5521 cgcctgtcga cgcggcgtcg gtcggccggg tctccgccgt ggacgtgatg ctgggcgccg
     5581 gcgaccgccc ggaccgctgg ctgctgcgca acgaccccga caaccacctg ctcttcgacc
     5641 accccgtcga ccatgtgccc ggcctggccc tgctggaggc cgccgaccag gcggcgcgcg
     5701 ccctgcgcgg cccggcgccg ttcacgccgg gccgtatcga ggcggtctac tggcgctacg
     5761 tcgagttcga ccagccctgc tggatcgcgg ccgagccgct gcccgagccc ggcgccgtcc
     5821 gcgtcaccgg cacgcaggag ggggaaccgg tcttccgcgt cgacttccgc gcgggggagt
     5881 gaggacagcc cgcggagtgg gcctcgcgcg ggcgcgtgtc cgtcagcgcg aggccggctc
     5941 ttccctcggc acccagcgca gggcgaggac gcccagggcg aggcagagca ggctcgccac
     6001 cccgatcgcg gcgtgcaggc cgtcgacgaa ggcgtcctcg gcccgcgcga cggtggccgc
     6061 cgtcaggccc ggctcggcga gggtctcgtc cagggtgccc gccacgtcgg gccccgtcag
     6121 ccggaacacc gcggcggcga gcgagccgag cagtgagatg cccagcgcgt tgcccagttc
     6181 gttgctggtc tcggcgatcg acgcggccga tccggcgcgc tcggccggca ccgccgccac
     6241 ggcggtgtcc gcgaccaggc tgaaggagaa gccgtagccg acacccgtga cgaccgagga
     6301 ggcgacgaac gccgccacgc cgtgctcggt ggtcgtcaac agcagcagga gcaggccgcc
     6361 tcccatgaag aagtgcgagg tgatcagcgc cgcccgcttg ccgatccgct cgaccacgcc
     6421 gctcgccccg acgcacgcgg cggtgagcac ggccgcgccc ggcagcacca gcagagccgc
     6481 cgtcaggaca ccgtggccga ggacggactg gaggtagacg ccggtgaggt agccggccgc
     6541 cgcccaggcg acgagtgaca gcacgccggt gaggatcgcg acggtgaaga cgcggtcctt
     6601 gaacagccgc aggtcgagca gcgggtgctc cagggcgagt tgccggcgga cgaaccacac
     6661 caggagcgcg acgcccagcg cgccggtgat cgcctccgtg gcggagaagg cctccgcggc
     6721 ggccgacttg accgcgtgga cggccagcag gatgccgacg gcggacagga cgacgctgag
     6781 cgcgtcgacc cggccggggt cggtggtgcg cacctcgcgc agcagcaccg gggcgaaggc
     6841 gacgaagagc accacgaccg ggatgttgat caggaacacc gatccccagc cgaaccggct
     6901 gagcagcagg ccgccgaaaa ccggtccgac cgcgaatccg gcggcgaagg tcgcggcgaa
     6961 gacgccgatg gcccgggagc gttgcctcgg gtcggggaag aggccgccga tgatcgccag
     7021 gccggcgggc agcagggtcg cgccgccgag gcccatcagg gcgcggcagg cgatgagcgt
     7081 ctccgggttc ggggagaacg cggcgcccgt cgagccgatg ccgaagacga cggcgccggt
     7141 cagcaggaat ctgagcctgc cgtaccggtc gccgaggctg ccgaaggcga tgagcaggga
     7201 cgagaccacg aagccgtaga tgtccaggat ccacagggcc tggtcggtgg tcggcgtgat
     7261 cgcggacgtg atgcccggca tcgcgaggta gagcacggag ccgtccatcg cgacgagggc
     7321 gaccgggccg agcacgacga gcagacccag ccaggctcgt cggtcggcac gttccgcgac
     7381 taagtcggag gaagtcacgg ggcaaccttt cgggagaagg cgcgaatcgg ttcgggaaac
     7441 gcgggaaacg ccgcatacgg cgtacgcgga atgcgtggcg tacggtgtac gcgaatttcg
     7501 tgcgtacggc gtacgcaagc tgttgggtat agtaacccat gaccatcaac gcgcaaggca
     7561 ggcaagcgag ttcacctggg ctgtcgcgac gtgccatcgt ggcgacagcg gttcgggtcg
     7621 ccgatgccga gggtgtcggc gcgctttcca tgcggcggct ggcgaacgaa ctgaactcgg
     7681 gcgtcatgtc gctctaccgc catgtggcga acaaggacgc gctgctgacc gccatcaccc
     7741 gggcggcctg tgacgaacac ccttatcccg agcccgcgcc cgcggactgg cgcgaggcga
     7801 tgcggctcgc cgcgcggcag gactggaagg tgtacaccgc ccacccctgg gcgctggtca
     7861 cgtccagctc cgcccggcac ttctcggaca cctcgtgcct ggactggatg atggacgcgc
     7921 tcgcggacct caccgacgac cccgacctgg cgcggtcgtt ctccatcgcc gtgtggtcgt
     7981 acgtgcaggg cgtgtgcatc cagcatgtgg cgcaagagct gctgcccgcc ggggacaggc
     8041 ccgagcagtc cccgctgcgg gccaccgagg aggacttcga gacggggctg gagaccctgc
     8101 tcgacgggat ggagcagcgc gcccggcacg ccggcccggc cctccgggaa ccgcgctccg
     8161 cgcccgcctt cccggccgcc ccggcacagt gaggcggacc ggcccggcgc gctatcgttc
     8221 ctgccatgat cgccatacgg ttcgaccgct tcggcggtcc cgaagtcctc acgcccgtcg
     8281 aagtcgccgc cccggaaccc ggtcccgacg agctgctcgt caccgtcgag gcggccgggg
     8341 tgaacttcgc cgacatccac caggcggacg gctcctacct ggaccccggc cagctgcccc
     8401 acatccccgg cagcgaggtc gtgggacgca ccgacgacgg ccgccgggtg ctggcgaagg
     8461 tctcccacgg ctacgcggag caggcggtgg ccaaggcgtc cgcggtcgtg gacatccccg
     8521 aggacctgga cgccggggcg gcgctcgcgc tgctggtgca ggggctgacg gcctggcatc
     8581 tgctgcgcac cgccgcccgc ctgggcaccg gcgagaccgt cgtcgtgcac tccgcggcgg
     8641 gcggtgtcgg aagcctcgcc gtacagctgg cccgggagtt cggcgccggg cgggtgtggg
     8701 cgcaggcctc cacgcccgag aagcggcgac tcgccctgga cctcggcgcg gacgccgccg
     8761 tggactaccc gctgccggag cgggcggagg tcatcctcga cgccgtcggc ggtgaactct
     8821 tcgaccacgc cctggactcg ctggcctccg gcggccggct cgtcacctac ggcaactccg
     8881 cgcgcaccgg cttcaccccc cttgacccgg cccggctcag ccggctcaac gccgccgtcg
     8941 tcggcttctg gctgcgcccc accctcgccg cgcccggcgc gttcgccggc ccgctgaagg
     9001 aactgctgtc gctgaccgcg cagggcaggc tcaagccggt ggtgcacccg gcgtacccgc
     9061 tcaccgaggc ccgccgcgcc cacgaggacc tgctggcccg gcgcacgacc ggcaaggtgg
     9121 tcctgcaccc ctgaacgtgg tgccgggacc tggaccgaac ggggtagcct ccagcatgac
     9181 cagtgatctt ctcgccggga aagtcgtcct cgtcaccggg gcgagcagcg gcataggcgc
     9241 cgccgcggcc aaggtgttct gccaggaggg cgcgacggtg gtgctcgccg cgcgccgcga
     9301 ggaccggctg gccgcgctga ccgaggaact gcgggcgaag ggggccgagg ccgcgcacgt
     9361 ggtgtgcgac gtctccgtcg ccgaggacgc cgcgcgggcc gtggagttcg ccgtcgcgga
     9421 gtacggacgg ctggacgggg ccttcaacaa cgccggtgtc ggcggcgacc gcacaccgct
     9481 gcacctgatg tcggacgacg tctacgacac cgtcatggac gtcaacgtcc gcggcgtgtg
     9541 gaactgtctg cggtacgaga tcgccgccat gctgaagggc ggcgggggcg ccgtcgtcaa
     9601 caacagcagc gtggccgggc tggtggcgat ccccgccgcc gcgccctaca tagccgccaa
     9661 gcacgcggtg gtcgggctga ccagggcggc ggccgacgag tacgcacagc aggggatcag
     9721 ggtgaacgcc gtcgccccgg gcacgacccg aagcgagatc accgaggact ggttccggcg
     9781 caacccgggc ctggagcaga cggtcaacgc gatgaccccg cagggccgta ccgccgagcc
     9841 ggaggagatc gccgccgccg cggcctggct gctgagcgac cgctgcccgt tcctgaccgg
     9901 gacggtgctg cccgtcgacg gcggtttcgt caaccagtga caatcacggc ggacgcggag
     9961 ccggaggcgt cacggtctcc cgctccgcgt ccctcgtacg ggccccggag gcgggccttg
    10021 gtaacatacc gagccttcgg tagtttaatc attgacctat cagtgttccg ggacggcggt
    10081 gccggccggg gcgctgggga gagtgtgggg gcggccgagt ggcgcggcag gagcgtgcga
    10141 tccgcacgcg tgagtccatt ctggaggcca tggcgagcct cttcatcgag gtcgggtacg
    10201 aggcggccac catcgcggcg ctggtcgagc ggaccggact gacccggggc gccctgtact
    10261 tccacttccc gaccaaggaa gccatggcgc acggcgtgct cgcccgcgcg gtgactcgcg
    10321 aggggatcct cccgcagacg tacaagctcc aggaatgggt cgacctcggg ctcctcctcg
    10381 cccaccggct gccccgggag ccgatgctgc gcgccgccgt ccagctgtcc gtcgacccca
    10441 aggcgcgcag cctgttcggc acccgctggc cggactggat cgacgtcggc caggacctgc
    10501 tcgaggaggc caaggaacgc ggcgagctcc agccgcacgc cgtgcccgcg gagatcgccc
    10561 ggctgaccgt cggcgcctgg accggggtgc agctggtgac cgaggcgctg ccggacggac
    10621 tcgggctctc cgaggagatc gcgcggctgt accagctgat cctgcccaac gtggccagca
    10681 tcggcgtcct cgccaaactg gacacctccc cctaccgcgg ggcgcggctg ctcgaggcgg
    10741 aggagcgctc cgcgtgaact cccttgactc gaagcgcact tcaggctgaa ggctcccgtg
    10801 cgtgccccgg cccgcgggca cggacgggaa ggaccaccat gcagtaccgc acgctcggca
    10861 cggacccggc gcgccgccgc ga
//
