LOCUS       NZ_LIVZ01000003        11380 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0107 scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_LIVZ01000003
VERSION     NZ_LIVZ01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0107
  ORGANISM  Streptomyces sp. TSRI0107
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 743720)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743720)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905845.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,745
            CDSs (total)                      :: 7,667
            Genes (coding)                    :: 7,467
            CDSs (with protein)               :: 7,467
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 85 of 200
            Pseudo Genes (incomplete)         :: 158 of 200
            Pseudo Genes (internal stop)      :: 13 of 200
            Pseudo Genes (multiple problems)  :: 51 of 200
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:19:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 162098
            Orig. end                         :: 173478
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVZ01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11380
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11380
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11380
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6380](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6380)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    242..256
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.0"
                     /tool="antismash"
     gene            complement(351..2117)
                     /locus_tag="AMK31_RS07935"
                     /old_locus_tag="AMK31_07850"
     CDS             complement(351..2117)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048312.1"
                     /locus_tag="AMK31_RS07935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_07850"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_079186760.1"
                     /transl_table=11
                     /translation="MSSATDTATTAGTDTGVPGRGTLRALLPALAGHRAMTARTCAAAV
                     LEHGSLVALVTLAAHTVGTAVIEGHTPSAGTATALVALVLVRALTTWREMDLSHDLAYR
                     VLAALRVRVFDGLARSAPARVAGRRSGDLAATAMADVEALEFFYAHTTAQLLAAATVFT
                     GGTAVLATVEPWLAAAVLPVAALLAAAPFADARGRAARGGRIRTAVARLSADTVETVDG
                     LRELLAFGALAARRRRLARRGREVGDAQRAEAVREAVAAAVRDLLIVAAVLGVVAAAAQ
                     SVTSGRLHGAWAPAAMALALSVLGPVAESARALSQAVTLRAAASRVDAAVKAPALAPPP
                     AAPRPLPPGPLGVRLHRVRFDYGGGPVLDGVELTVRAGQTLALVGASGAGKSTCAHLLA
                     RFWDPSEGAVQLIPADGDPVDVRDIGDAELRRAVALVGQETPLFHGTLAENLRLAAPDA
                     DDDLLAATARLCGVDRIAPMDTLVGERGSTLSGGQRARIALARALLARPRVLVLDESTA
                     HLDNASDARIAAALREEGRTTIVIAHRRATIRRADRIAVLEAGRITEEGTWEELTGRPG
                     SALNRSLAHITS"
     gene            complement(2114..3853)
                     /locus_tag="AMK31_RS07940"
                     /old_locus_tag="AMK31_07855"
     CDS             complement(2114..3853)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019328330.1"
                     /locus_tag="AMK31_RS07940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_07855"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_073939678.1"
                     /transl_table=11
                     /translation="MIVHPELRRAALRARRPLLTATVLQGAVTLTHLSQAALLAVALAD
                     LARGATGRLPWLLGAVLAVVAARAGLAMAQRRTATRAGARVRVDLRDELVAHLGQLGPA
                     HLTTARAGAVRTTLVDGVEGVDAYVSRYLPQLLITLTVPPLVLAAVAAVEPAALLGLVP
                     ALLLALAGPRAWDRLLAARGKEHWDTYEGLGADYLEALQGMPALRAAGAVGRTRERLER
                     RSAALHRATVAKLRVSLVDTGITDLAVQGGTAAAALLASWSAATGSTTATGTYLVLLLA
                     SECFRPVRDLAREWHAGYLGVSAADGIAALRTAEPAVQDTGPAPARWAVPPAVGFRNVT
                     FTYDGAPEPALHGVSFTAQAGRTTAIVGPSGAGKSTLLALLLRHHDPQDGRITLDGRPT
                     TEYALESLRQGIAVVSQETYLFHATIADNLRMARPDATDDDLTRAARVAGIHDEITALP
                     DGYATLLGERGATLSGGQRQRLALARALLADAPVLVLDEATSSVDERREADIVRELLNA
                     ASGRTVLVVAHRLAAVRHADRIVVLDAGRVDAVGDHAELVAAGGVYARLVEAGHAHRGE
                     LAA"
     gene            complement(3850..4992)
                     /locus_tag="AMK31_RS07945"
                     /old_locus_tag="AMK31_07860"
     CDS             complement(3850..4992)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048308.1"
                     /locus_tag="AMK31_RS07945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_07860"
                     /product="hypothetical protein"
                     /protein_id="WP_079186790.1"
                     /transl_table=11
                     /translation="MEHARQDHPSDLTAALARARTSEPPGPGTPAGPAVPAWPRPPRPI
                     PAAGPADLDLHALLRLSLAASDGSGRLRPASSAGALHPVDTELVVGTGCSLPPGRYGYD
                     PLRHRVHHLGPPPDGTPPGVTAELSVTARRTVSHYGHRAWPLLLLDTGHAAAALCLASR
                     ALATGKPEPRLDGLAENPLAAVHIPPRGPVRGPGGAPRPRPSASAPTPEELLARRSAPP
                     PLPGTPPPDALRAVLATAARAGGGDLRWCAAVGPPRPGLTELSTEGTTLRRRAAGDARP
                     TLAAWAAGQAWIADAGAVLLAHGCPADADAPHIRRTHLRAGFAVHLAHVTALRHGLAAR
                     PVGSWQRADLGAALGAAPGRDWIVHALALGTRHADEENTT"
     gene            complement(5001..6380)
                     /locus_tag="AMK31_RS07950"
                     /old_locus_tag="AMK31_07865"
     CDS             complement(5001..6380)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409979.1"
                     /locus_tag="AMK31_RS07950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_07865"
                     /product="YcaO-like family protein"
                     /protein_id="WP_073939680.1"
                     /sec_met_domain="YcaO (E-value: 2.7e-51, bitscore: 170.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTALPLEALVDPVCGIVRKVKPVEHPAGAPPRYTALTAEISDARR
                     LGLWPADRVSLGTTFGDPEQARVAAIAEGVERYCGNRVPPPGHPDAPLRATAAELAEKG
                     LRLYGPDDLPAYAQWQYTREKFPYRRLTADTPALWTRGTERLPGGRTEEVWAPVALTHL
                     NWRQGDLRELPRTHHLNYAGIATGRGLEDAVERALLEIVERDALELWWHLDGPARGIDP
                     ASVPGLEDDLAGSALDVHLVEMPSEFAPCMAALVHDPRLGLYAAGFACKYDPAEAARKA
                     VLEAVHTWVFTQGLTDSDGWVFQAVEAGLLARGLYLEHRADGRYLDACGPRFEHVRDLG
                     AHVQVWLDERMAAEARRFTDPAHGTVPVDAVEPGNRERLESALHTGGHRVMTFDLTTED
                     VAETALRVARVLVSGLLPNAPAAFGYFGCPRLAEAAVRRGWRTTAPTAPEDFTLAPPPH
                     M"
     gene            complement(6377..7885)
                     /locus_tag="AMK31_RS07955"
                     /old_locus_tag="AMK31_07870"
     CDS             complement(6377..7885)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004117.1"
                     /locus_tag="AMK31_RS07955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_07870"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_073939681.1"
                     /transl_table=11
                     /translation="MSSIAEEAPTTSIGAAVEGLATDVRLPGGDGPFPAVLLRTPYDRA
                     RHRAEARGWARRGLAAVVQDVRGRFGSPGEWQPYRHETADGTATARWIRRQPWSDGRLV
                     AVGASYAAHCALVLALEAPGDARPDAVIAAVPALGVAETAREPSGVERLLARAGWWAAH
                     GDRRDSDESALGKALAADPGLLTHLPLTGLPERLGRDLPSWAGLWRHRDRGRLVSRAAH
                     ATMPLLAVGGHHDHFTEDTVALWHAWGGPSARLLLGPWGHSLVAAPGTDAGPAHRVNLG
                     DLYVRWAHRALTGELAPGRHGALALGGGDLWLPAGTDGEPRTQRLRLLHGADFTADPDR
                     PVRSDDLTVPTGGRPDRCLLAGPPLPRPLDVVGSARVRLRATADTPSADWAARLVALTP
                     EGTADRLAVGVLRREQPADRESEFTIELGRIARRLPAGTRLRLEIAGHHFPAHARNPHT
                     GDDPTTTARLLASRRQVDPESVALRLPVLPSRPTSTEPAKEILR"
     gene            complement(7882..8736)
                     /locus_tag="AMK31_RS07960"
                     /old_locus_tag="AMK31_07875"
     CDS             complement(7882..8736)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048303.1"
                     /locus_tag="AMK31_RS07960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_07875"
                     /product="hypothetical protein"
                     /protein_id="WP_073939682.1"
                     /transl_table=11
                     /translation="MFRSRLVPGVEVVTVPGRGLAVRTADGEFLSVRTQDADQDSLLAL
                     LSGGTAAPLDEELGRVVGAFEQAGYLMDEPQHVEWPAARRDVRLLGAPALTEPLAAHLT
                     ALGAEPRTTPAVTASDPSPGKIEDLVDGDPAAVVWCLDGPVPDGLWEAADRLPEHGVAW
                     LRCHREGWQAYVEPLAAAPGDVTAAHIRARRLAATPAHRELAAYWSGPRTSGAPVRLTV
                     PAAALLAALLADDLGRWATGAPDPAGLPARRRLRRVDLRTLTVTEHPVLPVPDVAPMPR
                     KDR"
     gene            complement(8831..8944)
                     /gene="amiA"
                     /locus_tag="AMK31_RS41405"
     CDS             complement(8831..8944)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048301.1"
                     /locus_tag="AMK31_RS41405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_241270818.1"
                     /transl_table=11
                     /translation="MDNEQVFAPIADPGQLAHDSASHSNALVENPFEDTEE"
     misc_feature    8948..8963
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 16.48"
                     /tool="antismash"
     misc_feature    9003..9016
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.45"
                     /tool="antismash"
     misc_feature    complement(9003..9018)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.88"
                     /tool="antismash"
     gene            complement(9004..9823)
                     /locus_tag="AMK31_RS07965"
                     /old_locus_tag="AMK31_07880"
                     /pseudo=""
     CDS             complement(9004..9823)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004114.1"
                     /locus_tag="AMK31_RS07965"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_07880"
                     /product="ABC transporter ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSESVGIHVEGVHFGYPARPVLRGVDMTVGPGELTALIGLNGCGK
                     STLLRLAAGLLRPDRGRVLLGGDDLAALSRRATARRVALLHQSAPAVPGMTVRHLVRQG
                     RYAARGPLGMLREGDDPVVHRALRDVGVEQWADRDVDALSGGERQRVRLAMALAQDTRV
                     LLLDEPTTYLDLHHQLDVLQTVVRLREERGLTVVMVLHDLAHAARFAERVVALRDGRVV
                     ADGTPKEVVTPGLLADVLKVAGRVGRDPEGGWPVCYPDHPLPAGI"
     misc_feature    complement(9005..9007)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(9843..10850)
                     /locus_tag="AMK31_RS07970"
                     /old_locus_tag="AMK31_07885"
     CDS             complement(9843..10850)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019328328.1"
                     /locus_tag="AMK31_RS07970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_07885"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_073939683.1"
                     /transl_table=11
                     /translation="MKRRFAALAALAVVCAGLELVAGRGMSPGVVWDVLGGGGDMTERH
                     ILLRLRLPRTLVALGAGACLAVAGLVLQSALRNPLAGPEVTGVTPGAVLGAVTATALGL
                     AGWDSPLAVVLAACAGGCAGAALLWLLAGRGRGDPAQTAVHGVLVSAVLGGLTAMVLLV
                     EPGELGSVVQWLLGTTEGRVWQHWHLLWPWALVWGAAAWLLAGPLTLLRCGDDIALAAG
                     LSARRARTLALSCAVALTAGAVAAVGALGFVGLLVPHLAVALFGADLRVSLPGAALVGA
                     VVVCGADAVAQLSSRLLAAALDSARLTLPVGALTACLGAALLLVVVRRTPSRSF"
ORIGIN
        1 cacgcgtccg gcgaggcggt gacggcggta gacggcgcgg aagcgcgcgg tgttctcggc
       61 ggcggcctcg gggagcagca cgttgagcgg cgatcccagc gcgtcgacga gcgagtgcag
      121 gaacggctgt gcctccagca gtgaggcgag cggcggaccc gccctcggcc tcagatacaa
      181 gggcgcgtcc acgtaagggc ccctcccggc gtcgaccgac catcgttgcg acaatcgttc
      241 gggaatagtc gtttcccact acacccgccg ctaagcctcg ccgcgcccct ggccggtgtg
      301 ccgccgctgc ttgtcacctt ctgaacagac cggcgtgcag gccgagttgt tcacgaggtg
      361 atgtgggcga ggctgcggtt gagtgcgctg ccggggcgtc cggtgagctc ctcccaggtg
      421 ccctcctccg tgatgcggcc ggcttcgagg acggcgatgc ggtcggcgcg gcggatggtg
      481 gcgcggcggt gggcgatgac gatcgtggtg cggccctcct cccgcagcgc ggccgcgatt
      541 cgggcgtcac tggcgttgtc caagtgggcg gtggactcgt ccagtacgag gactctgggc
      601 cgggcgagca gcgcgcgggc gagggcgata cgggcccgct ggccgccgga cagggtggat
      661 ccgcgctcac cgacgagggt gtccatcggg gcgatccggt cgacaccgca caggcgggcc
      721 gtcgcggcga gcaggtcgtc gtccgcgtcg ggtgcggcga gccgcaggtt ctcggcgagg
      781 gtgccgtgga agaggggcgt ctcctggccc accagtgcga cggcacgccg cagttccgcg
      841 tcgccgatgt cgcgtacgtc gacgggatcg ccgtcggcgg gaatgagctg gaccgctccc
      901 tccgacgggt cccagaagcg ggccagcagg tgcgcgcagg tcgatttccc ggcgcccgag
      961 gcgcccacga gagccagcgt ctgccccgcc cggacggtca gctcgacgcc gtccagtacg
     1021 gggccgcccc cgtagtcgaa cctcacccgg tgcagccgca cccccagcgg gccggggggc
     1081 agcgggcgcg gtgcggcggg tggcggggcg agcgcgggag ccttgacggc cgcgtcgacg
     1141 cgggaggcgg cggcgcgcag cgtcacggcc tggctcaggg cacgggccga ctccgcgacc
     1201 ggaccgagga cggacagggc gagcgccatc gccgccgggg cccacgcgcc gtgcagccgg
     1261 ccggaggtca cggactgggc cgcagcggcc accacgccga ggacggcggc cacgatgagg
     1321 aggtcgcgga ccgcggcggc gaccgcttcc cgcacggcct cggcacgctg ggcgtcgccc
     1381 acctcccgtc ccctgcgcgc cagtcggcgg cgtcgtgcgg ccagggcgcc gaaggcgagc
     1441 agttcgcgca gcccgtcgac ggtctccacc gtgtcggccg acagcctcgc gacggccgtc
     1501 cggatgcggc cgccgcgcgc cgcgcgtccg cgcgcgtcgg cgaagggggc cgcggcgagc
     1561 agcgcggcga ccggcagcac ggccgccgcc agccagggct ccaccgtggc gaggaccgcg
     1621 gtgccgccgg tgaaaaccgt cgcggcagcc aggagttggg cggtggtgtg ggcgtagaag
     1681 aactccagcg cctccacgtc ggccatggcg gtggcggcca ggtcgccgct gcgccggccg
     1741 gcgacacggg cgggtgcgct gcgggcgagt ccgtcgaaga cgcgtacgcg cagcgcggcc
     1801 aggacccggt aggccaggtc gtgtgacagg tccatctcgc gccaggtcgt cagggcgcgt
     1861 acgaggacga gggcgacgag tgcggtggcg gtgccggccg agggggtgtg cccttcgatg
     1921 acggccgtgc ccacggtgtg cgcggccagc gtcaccagcg cgacgaggga gccgtgttcg
     1981 aggacggcgg cggcgcaggt gcgggccgtc atggcgcggt gcccggcgag ggcggggagc
     2041 agagcgcgca gtgtgccccg gccgggcacg cccgtgtcgg tgccggcggt ggtggcggtg
     2101 tcggtggcgc tgctcatgcg gcgagttctc ctcggtgggc gtggccggcc tcgacgagtc
     2161 gcgcgtagac gccaccggct gcgaccagtt cggcgtggtc tccgacggcg tccacccgtc
     2221 ccgcgtcgag gacgacgatg cggtcggcgt gccggacggc ggcgagccgg tgggcgacca
     2281 ccaggacggt ccggccgctc gcggcgttca gcagttcccg gacgatgtcc gcctcccggc
     2341 gttcgtccac cgaactggtg gcctcgtcga ggacgagcac cggcgcgtcc gcgagcaggg
     2401 cccgggcgag ggcgagccgc tggcgctgcc cgccggacag ggtggcgccg cgttcgccga
     2461 ggagggtggc gtagccgtcg gggagggcgg tgatctcgtc gtggatgccg gcgacgcggg
     2521 ccgcgcgcgt caggtcgtcg tcggtcgcgt ccgggcgggc catgcgcagg ttgtcggcga
     2581 tggtggcgtg gaagaggtac gtctcctggg agaccacggc gatgccctgc cgcagcgatt
     2641 cgagcgcgta ctcggtcgtg gggcgcccgt cgagggtgat ccggccgtcc tgcgggtcgt
     2701 gatggcgcag gagcagggcg aggagcgtcg acttgcccgc gccggacggg ccgacgatcg
     2761 cggtggtgcg tcccgcctgc gcggtgaagg agacgccgtg gagggcgggc tccggtgcgc
     2821 cgtcgtacgt gaaggtgacg ttccggaaac ccacggcggg tggtaccgcc cagcgcgcgg
     2881 gggccggtcc ggtgtcctgg acggcgggct cggcggtgcg cagtgccgcg atcccgtccg
     2941 cggccgacac gcccaggtag ccggcgtgcc actcgcgggc caggtcgcgg acggggcgga
     3001 agcactcgga ggccagcagc agcaccaggt aggtgccggt cgccgtggtg gagccggtgg
     3061 cggccgacca ggaggcgagg agcgcggcgg cggcggtgcc gccctggacg gcgaggtcgg
     3121 tgatgccggt gtcgaccagg gacacccgca gtttggcgac ggtcgcccgg tgcagcgccg
     3181 ccgagcgccg ctccaggcgt tcacgggtgc ggccgaccgc gccggcggca cgcagggcgg
     3241 gcatgccctg cagggcctcc aggtagtcgg cgccgagtcc ttcgtaggtg tcccagtgct
     3301 ccttgccccg tgcggccagg agccggtccc aggcgcgcgg cccggccagg gcgagcagca
     3361 gggcggggac gagaccgagg agggccgcgg gttcgaccgc ggcgacggcg gcgagcacca
     3421 ggggcggcac ggtgagggtg atcaggagct ggggcaggta gcgggagacg tacgcgtcga
     3481 cgccctccac cccgtcgacg agggtggtgc gtaccgcccc ggcgcgcgcg gtggtcagat
     3541 gtgcggggcc caactgtccg aggtgggcga cgagttcgtc gcgcaggtcc accctgaccc
     3601 gggctccggc acgggtggcg gtgcgccgct gcgccatggc gaggccggcg cgggcggcga
     3661 cgacggcgag caccgcgccg agcagccacg ggagccggcc ggtggcgccg cgggccaggt
     3721 cggcgagcgc gacggccagc agtgcggcct gcgacaggtg ggtgagggtg acggcgccct
     3781 ggaggacggt ggcggtgagc agggggcgcc gtgcgcgcag ggcggcgcgg cgcagttcgg
     3841 ggtggacgat cacgtggtgt tctcctcgtc ggcgtggcgg gtgccgaggg ccagggcgtg
     3901 gacgatccag tcccggcccg gcgcggcgcc cagcgcggcg cccaggtcgg cccgctgcca
     3961 ggagccgacg ggccgggcgg ccagcccgtg gcgcagggcg gtgacgtggg ccaggtggac
     4021 ggcgaatccg gcccgcaggt gggtgcggcg gatgtgcggc gcgtcggcgt ccgcggggca
     4081 gccgtgggcg aggaggacgg cgcccgcgtc ggcgatccac gcctggcccg cggcccaggc
     4141 cgcgagcgtc ggccgggcgt ccccggcggc gcgccggcgc agtgtcgtgc cctcggtcga
     4201 cagttccgtc aggccgggcc gtggcgggcc gacggcggcg caccagcgca ggtcgccacc
     4261 gcccgcccgt gcggcggtgg cgaggaccgc ccgcagcgcg tccgggggtg gggtgcccgg
     4321 cagcggtggc ggggcgctgc ggcgggccag cagctcctcg ggggtgggcg cgctcgccga
     4381 cggccggggg cgtggtgcac cgccgggccc gcgcaccggg ccgcgcggag ggatgtgtac
     4441 ggcggcgagc gggttctccg cgagtccgtc cagccgtggc tcgggcttgc ccgtggcgag
     4501 tgcgcgggac gcgaggcaga gcgccgcggc ggcgtggccg gtgtccagga gcagcagggg
     4561 ccaggcgcgg tggccgtagt gggagaccgt gcggcgtgcg gtgacggaga gttcggcggt
     4621 cacgcccggt ggcgtgccgt cgggtggcgg gccgaggtgg tggacgcggt ggcgcagtgg
     4681 gtcgtagccg tagcggcccg gcggcaggga gcagccggtg cccacgacga gttcggtgtc
     4741 caccgggtgc agggcaccgg cggaggatgc gggccgcagc cgcccggagc cgtccgaggc
     4801 ggccagggag aggcgcagca gggcgtgcag atcgaggtcg gccgggccgg ccgccggtat
     4861 cgggcgcggt ggccgcggcc aggcggggac ggcgggtccg gcgggtgtgc cggggccggg
     4921 aggctcggag gtgcgggcgc gggcgagcgc ggcggtgaga tccgacgggt ggtcctgacg
     4981 ggcgtgttcc acggcggccc tcacatgtgc ggcggaggcg ccagcgtgaa gtcctccggc
     5041 gcggtgggcg cggtcgtacg ccagccccgt cggacggcgg cctcggcgag ccgcgggcag
     5101 ccgaagtagc cgaaggcggc gggggcgttg gggaggagcc cggagacgag gacccgggcg
     5161 acgcgcaggg ctgtctcggc gacgtcctcg gtggtgaggt cgaacgtcat gacacggtgg
     5221 ccgcccgtgt gcagcgcgct ctccagccgt tcccggttgc cgggctcgac cgcgtcgacc
     5281 ggcacggtcc cgtgggcggg gtcggtgaag cggcgggcct cggccgccat ccgttcgtcc
     5341 agccacacct ggacatgcgc gcccaggtcc cgcacatgct cgaaccgcgg gccgcacgcg
     5401 tcgagatagc ggccgtcggc gcggtgctcc aggtagagtc cgcgggcgag cagcccggcc
     5461 tcgacggcct ggaagaccca gccgtcggag tcggtgaggc cctgggtgaa gacccaggtg
     5521 tggacggctt cgagaaccgc tttgcgggcg gcctcggccg ggtcgtactt gcaggcgaaa
     5581 ccggccgcgt acaggccgag gcgcgggtcg tggacgagcg cggccatgca gggggcgaac
     5641 tccgagggca tttcgacgag gtggacatcg agcgcggatc cggccaggtc gtcctcgagg
     5701 ccgggcacgc tggccgggtc gatgccgcgg gccggtccgt cgaggtgcca ccacagttcc
     5761 agggcgtcgc gttcgacgat ctccagcagg gcgcgttcga cggcgtcttc gaggccccgc
     5821 ccggtggcga tgcccgcgta gttgaggtgg tgggtgcgag gcagctcgcg caggtcgccc
     5881 tggcgccagt tgaggtgggt gagggcgacg ggcgcccaga cctcctccgt ccgtccgccg
     5941 ggcagccgct ccgtaccccg cgtccagagg gccggggtgt cggcggtgag gcggcggtaa
     6001 ggaaacttct cgcgggtgta ctgccactgc gcgtaggcgg gcaggtcgtc ggggccgtag
     6061 agccgcaggc ccttctcggc cagttcggcc gcggtggcgc gcaggggggc gtcggggtgg
     6121 ccgggcggcg gcacgcggtt gccgcagtac ctttccaccc cctcggctat cgcggcgaca
     6181 cgggcctgct cggggtcgcc gaaggtggtg cccagggaga cccggtcggc gggccacaga
     6241 ccgagtctgc gggcgtcgga tatctcggcg gtgagcgcgg tgtagcgcgg cggtgcgcca
     6301 gcggggtgtt ctacgggctt caccttgcgg acgatgccgc agacggggtc gacgagtgct
     6361 tccagcggca gcgccgtcat cgcaggatct ccttggcggg ctcggtggac gtgggacggg
     6421 aagggagcac gggaagcctc aacgccacgc tctccgggtc gacttgccgc cgggacgcga
     6481 gcagccgggc ggtggtcgtc gggtcgtcac cggtgtgggg gttgcgggcg tgggccggga
     6541 agtggtgtcc ggcgatctcc aggcgcagcc gtgtgccggc cggcagccgg cgcgcgatcc
     6601 ggccgagctc gatggtgaac tcgctctccc ggtccgccgg ttgctcgcgt cgcaggacgc
     6661 cgacggcgag tcgatcggcg gtgccttcgg gtgtgagggc gacgagccgg gcagcccagt
     6721 cggcggacgg ggtgtcggcg gtggcccgca gccgtacccg cgccgatccg acgacgtcga
     6781 gcgggcgcgg caacggcggg ccggccagca ggcagcggtc ggggcgtccg ccggtgggga
     6841 cggtcaggtc gtcggagcgg acgggccggt cggggtcggc ggtgaagtcg gcgccgtgca
     6901 gcagccgcag ccgctgcgtg cggggctcgc cgtcggtgcc ggcgggcagc cacaggtcgc
     6961 cgccgcccag cgccagcgcg ccgtggcggc ccggggcgag ctcgccggtc agggcgcggt
     7021 gggcccagcg tacgtacagg tcgccgaggt tcacccggtg ggcgggcccc gcgtcggttc
     7081 cgggcgcggc gacgaggctg tgcccccagg ggcccagcag cagccgtgcc gagggcccgc
     7141 cccaggcgtg ccacagcgcg acggtgtcct cggtgaagtg gtcgtggtgg ccgcccaccg
     7201 cgagcagggg catcgtggcg tgcgccgccc gcgacacgag ccggccgcgg tcgcgatgcc
     7261 gccacaggcc cgcccaggag ggcagatccc ggcccagccg ctcgggaagg ccggtgagcg
     7321 gcagatgggt gagcagcccc gggtcggcgg cgagggcctt gcccagcgcg ctctcgtcgg
     7381 agtcccgtcg gtcgccgtgg gcggcccacc agcccgcgcg ggccagcagc cgttccacgc
     7441 cggagggttc acgcgccgtc tcggcgactc ccagggcggg tacggcggcg atgacggcgt
     7501 ccgggcgggc gtcgccgggc gcttcgagag cgaggacgag ggcgcagtgg gcggcgtagg
     7561 aggcgccgac ggcgacgagc cgtccgtcgc tccagggctg ccgcctgatc cagcgggccg
     7621 tcgccgtccc gtcggcggtc tcgtgccggt acggctgcca ctcccccggc gagccgaacc
     7681 gcccccgcac gtcctggacg acggcggcga gaccccggcg ggcccagccg cgcgcctcgg
     7741 cccggtgccg ggcacggtcg tagggggtgc gcaggagtac ggcggggaag gggccgtcgc
     7801 cgccggggag gcggacgtcc gtggcgaggc cttcgacggc cgcgccgatg gaggtcgtcg
     7861 gtgcttcctc ggcgatggag ctcatcggtc cttcctcggc atcggtgcga cgtcgggcac
     7921 cgggaggacg gggtgttcgg tgacggtcag cgtgcgcagg tccacgcgtc ggagccgccg
     7981 ccgggccggg aggccggccg ggtcgggggc gccggtggcc caccggccga ggtcgtcggc
     8041 gagcagagcc gcgagcagcg cggcggcggg cacggtgagc ctgaccggtg ctccggaggt
     8101 gcggggcccg ctccagtagg cggcgagctc gcggtgggcg ggggtggcgg cgagtcggcg
     8161 agcccggatg tgggcggcgg tgacgtctcc gggggcggcg gcgaggggtt cgacatacgc
     8221 ctgccagccc tcgcggtggc agcgcagcca ggcgaccccg tgctcgggca gccggtcggc
     8281 ggcctcccac aggccgtcgg gtacgggtcc gtcgaggcac cacacgacgg cggcggggtc
     8341 tccgtcgacc agatcctcga tcttccccgg tgacgggtcg gaagcggtca cggccggagt
     8401 cgttcgcggc tcggcgccca gtgcggtcag gtgcgcggcc agcggttcgg tcagtgccgg
     8461 ggctcccagg agccggacgt cccggcgggc ggcgggccac tccacgtgct gcggctcgtc
     8521 catcaggtag ccggcctgct cgaaggcccc tacgacgcgg ccgagttcct cgtccagtgg
     8581 ggcggcggta ccgccggaga gcagggcgag cagggagtcc tggtccgcgt cttgcgtcct
     8641 gacgctgagg aactccccgt ccgcggttcg gacggctagg ccccgcccgg gcacggtgac
     8701 gacttcgaca ccgggtacga gccggctgcg gaacacgacg atcctcctga gacgaacggg
     8761 gcctgggacg ggtgcgggcc cgcccggaag gcaacgggcg ggcccacggt cgagggtgct
     8821 gtgccctcgc ttactcctcg gtgtcctcga acgggttctc gacgagggcg ttggagtggg
     8881 aagccgagtc gtgagccagc tgacccgggt cggcgatcgg cgcgaagacc tgctcgttgt
     8941 ccatgaactc tccttttttc tacggggaga tgcggcgttc ggtcgatcgc cgcactgaag
     9001 gcactaatga atatgatttt catattccag cggggagagg gtgatctggg taacacaccg
     9061 gccagccccc ctcgggatcc cggccgaccc ggccggccac cttcagcacg tccgccagca
     9121 accccggcgt gacgacctcc ttcggcgttc cgtcggccac cacccggccg tcgcgcaggg
     9181 cgacgacgcg ctcggcgaac cgggcggcgt gggccaggtc gtgcagcacc atcaccacgg
     9241 tcagcccgcg ttcctcgcgc agacggacca ccgtctgcag cacgtcgagc tggtggtgca
     9301 ggtccaggta ggtggtcggc tcgtcgagca gcaggacgcg ggtgtcctgg gcgagcgcca
     9361 tcgcgagccg cacgcgctgc cgttcacctc cggagagcgc gtcgacgtca cggtcggccc
     9421 actgctccac cccgacgtcg cgcagcgccc ggtgcacgac ggggtcgtcg ccctcgcgca
     9481 gcatgcccag cgggccacgc gccgcgtaac gcccctgccg caccaggtga cgcaccgtca
     9541 tacccggtac ggcgggcgcg gactggtgca gcagcgccac tcttcgggcc gtggcgcgcc
     9601 gggagagcgc ggcgaggtcg tccccgccga gcagcacgcg ccctcgatcg ggccgcagca
     9661 gcccggcggc cagccgcagc agggtcgact tgccgcagcc gttcaggcct atgagagcgg
     9721 tcagttcgcc cggcccgacg gtcatgtcga cgccccgcag cacgggtcgt gcggggtagc
     9781 cgaagtggac cccctcgacg tggatcccca cggactcgct catgatctgt cctccgtcga
     9841 cttcagaacg aacggctcgg cgtgcggcgc acgacgacca gcagcagcgc cgcaccgagg
     9901 caggcggtga gcgccccgac cggcagagtg aggcgtgcgg agtccagcgc cgcggccagc
     9961 agccgcgagg acagctgggc cacggcgtcc gccccgcaca ccacgaccgc gcccaccagg
    10021 gccgcgccgg gcaggctcac ccgcaggtcc gcgccgaaga gggccacggc caggtgcggg
    10081 acgagcaacc ccacgaagcc cagcgcccct acggccgcca ccgcgcccgc cgtcagcgcc
    10141 accgcgcacg acagggccag ggtccgggcc cgccgcgcgg agagcccggc ggcgagggcg
    10201 atgtcgtccc cgcagcgcag cagggtcagc gggccggcca gcagccaggc cgcggcgccc
    10261 cagacgagcg cccacggcca cagcaggtgc cagtgctgcc acacccggcc ctccgtggtg
    10321 cccaacagcc actggacgac gctgccgagt tcgcccggct ccacgaggag caccatggcg
    10381 gtgagcccgc cgagcaccgc cgacacgagc acgccgtgca cggcggtctg cgcgggatcg
    10441 ccacggccac ggccggcgag cagccacagc agcgcggctc cggcgcagcc gcccgcgcac
    10501 gcggcgagga ccacggccag gggcgagtcc cacccggcga gccccagagc cgtcgcggtg
    10561 acggccccga ggaccgcccc cggcgtcacg cccgtcacct caggtccggc gaggggattg
    10621 cgcagcgcgg actgcaggac cagcccggcc accgcgaggc aggcgcccgc gcccagggcc
    10681 accagcgttc tgggcaggcg gagccggagg aggatgtggc gttccgtcat gtcgccaccg
    10741 ccgcccagga cgtcccagac gacgcccggg gacatgccgc gccccgccac cagctccagc
    10801 ccggcgcaca cgacggccag tgccgcgagt gcggcgaaac gacgcttcac cgtgcggcct
    10861 ccgttccagt cggctcggag ggggccgggg aggcgcccct gggaccgtgg agcccgggct
    10921 cgtctccacg agcgcgcttc cgcgtggaac ccttcgcgcc tgggccgccc gaccgcatca
    10981 gagcgacccc ggccggcacc cccagcaagg ccgtccaggc gccggccggc gtctccacgg
    11041 gtgccagcgc cagccgggcc gggacgtcgg cgaccgccac gacgacggca ccccaggcgg
    11101 cggaccacgg cagccagcgc acggcccccg tcgcggggtt gaaccaccgg gcgaggtgcg
    11161 gggcgaggaa tcccacccac gcgacgggcc cgcacacagc cgtgacaggc gcgatcagca
    11221 gcacggcgat ggcgagcgcg gccatgcgtg cccgctgggc gcgcacaccc agcgcctcgg
    11281 cgtcctcgtc gcccagccgc atcaccgaga gcaccggcgc gcacagcacg agggcggggg
    11341 ccgcgaccag cagccacggc cacagccccg cgacgtcgtc
//
