LOCUS       NZ_LIVZ01000002        11320 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0107 scaffold2, whole genome shotgun sequence.
ACCESSION   NZ_LIVZ01000002
VERSION     NZ_LIVZ01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0107
  ORGANISM  Streptomyces sp. TSRI0107
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 817466)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817466)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905845.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,745
            CDSs (total)                      :: 7,667
            Genes (coding)                    :: 7,467
            CDSs (with protein)               :: 7,467
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 85 of 200
            Pseudo Genes (incomplete)         :: 158 of 200
            Pseudo Genes (internal stop)      :: 13 of 200
            Pseudo Genes (multiple problems)  :: 51 of 200
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:19:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 244596
            Orig. end                         :: 255916
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVZ01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11320
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11320
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11320
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6320](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6320
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    1197..1212
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.94"
                     /tool="antismash"
     misc_feature    complement(1199..1212)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.39"
                     /tool="antismash"
     gene            1201..2076
                     /locus_tag="AMK31_RS05315"
                     /old_locus_tag="AMK31_05215"
     CDS             1201..2076
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923596.1"
                     /locus_tag="AMK31_RS05315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05215"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_073939544.1"
                     /transl_table=11
                     /translation="MIVLVRRIVAVCALGAALSACGAPEAARTASRAAPPASASASPAS
                     PSPGSPSPARPPTLAPGPGGMTPVFHNGPRTPGDKTVALTFDADMTADQGPRAAMGERF
                     DNPRLIATLRALRVPATVFMTGRWAEEYPVQARSIGRDPLFEVANHSYSHYAYTEDCYG
                     LPTVDEPLMRADVERAYAAFRAVGVPDARPYFRFPGGCYDRRALRTLAGTGVTAVQWDV
                     VGGDAFATDADAVARQVLDGVRPGSVVVLHCTTSAAPATERAIRTIVPELRHRGFRFVK
                     VSELISAAPR"
     gene            2097..2285
                     /locus_tag="AMK31_RS05320"
                     /old_locus_tag="AMK31_05220"
     CDS             2097..2285
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034064.1"
                     /locus_tag="AMK31_RS05320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05220"
                     /product="hypothetical protein"
                     /protein_id="WP_073939198.1"
                     /transl_table=11
                     /translation="MSEAEVNDYCLIDATRPPKADGPPYAECVLCREPTEYAESYKGIT
                     LCPVCEWQEAQRTACSG"
     gene            2338..3054
                     /locus_tag="AMK31_RS05325"
                     /old_locus_tag="AMK31_05225"
     CDS             2338..3054
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125241.1"
                     /locus_tag="AMK31_RS05325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05225"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_073939199.1"
                     /transl_table=11
                     /translation="MTHPSSVPGDSPFRPVRSARDEAPQFVLPLVVRIERAEPPARTDA
                     LETAAKAVLVMLSDERSLRDGEWAQAVRDWQDARIRKVVRRARGAEWRRAETLPGITVT
                     GKSAEVRVFPPVPLDGWPKELAKLQVSGTDLDDPERPTAADPGTPVLWMSPEVEMSAGK
                     AMAQAGHGAQLAWWALPDEERAAWRDAGFPLAVRTADPARWRALTASGLPVVRDAGFTE
                     IAPGSCTVVAQHPALR"
     gene            complement(3179..3562)
                     /locus_tag="AMK31_RS05330"
                     /old_locus_tag="AMK31_05230"
     CDS             complement(3179..3562)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018530404.1"
                     /locus_tag="AMK31_RS05330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05230"
                     /product="hypothetical protein"
                     /protein_id="WP_073939200.1"
                     /transl_table=11
                     /translation="MANTQEREGKTAQAARTAKTAASKTGDKAASSGTTAARAARSASE
                     GGSRAVAAAGTALATTKDTVVSTAGQVATAATTAWTVIKARKLIATGAATGVTALATLS
                     FAAGRVSARRGHGPITRLTGGRF"
     gene            3722..3865
                     /locus_tag="AMK31_RS40885"
     CDS             3722..3865
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992114.1"
                     /locus_tag="AMK31_RS40885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_198948424.1"
                     /transl_table=11
                     /translation="MPGIINRIKQYSRTPQGRRAIASARRAASDPRTRAQARTLLGRLR
                     RR"
     gene            3997..4836
                     /locus_tag="AMK31_RS05335"
                     /old_locus_tag="AMK31_05235"
     CDS             3997..4836
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004991966.1"
                     /locus_tag="AMK31_RS05335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05235"
                     /product="DUF1206 domain-containing protein"
                     /protein_id="WP_073939201.1"
                     /transl_table=11
                     /translation="MATGASVTRGKGAARARRAANGSVVDAGARWGLVARGTIYLLIGL
                     LALRIAHGGGARQADSSGAVQELARQPFGEALVWAIGIGLIGMALWRLSEALFGASGPD
                     GRKASKRALNAGRCVFYGFVASSVLSFVTSSRNDEGSDATSRDLTARALELPLGRWLVG
                     VAGLVVLGVGVGIGVRAVRRTFHKHLKRADMSRRVRRVVDVLGVTGGVARGLVYAAAGV
                     FVVIAAVDYDPDRAKGLDGTLRALADAPAGPWLLSLVALGLAVFGLFSVALARYRRV"
     gene            5001..6320
                     /locus_tag="AMK31_RS05340"
                     /old_locus_tag="AMK31_05240"
     CDS             5001..6320
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327585.1"
                     /locus_tag="AMK31_RS05340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05240"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_073939202.1"
                     /sec_met_domain="DUF692 (E-value: 3.7e-99, bitscore: 326.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRLGTGIGWRPEIADAVEAMPGIDWVEVVAENVCLGHLPDSLRR
                     LRERGVTVVPHGVALGLGGADRPDPGRLSALAERAEALGSPLVTEHIAFVRAGGPLTAS
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALFSWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPAKALAELPVEAIAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTDLASRVAPAGVLLERDENFPEPAELEGELAAIREALRKGRGQSRAA
                     GAATAGTSAPRPAATAATAVPDGVRERLGLAQAALLSALVAGTPAPEGFDRARLRVQTR
                     ALAGKRAGVVAKVAPELPDLLGDAYRTEFLAYAHRRPMTGGYRRDALDFAEHLLDSGRL
                     QARVRREMRAWWRERSGPAPRSARPAARLARGARRVLLGR"
     gene            6440..7216
                     /locus_tag="AMK31_RS05345"
                     /old_locus_tag="AMK31_05245"
     CDS             6440..7216
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657155.1"
                     /locus_tag="AMK31_RS05345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05245"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073939203.1"
                     /transl_table=11
                     /translation="MFWVILLLLAWVAAGTACTLLCLAAVHAAGTDADTGGTHELTLYE
                     AAFLSGGPARVAELTLVSMARQRRLLLAHTGWATVVDPRGRDEMERSVIGAIGPEGQSP
                     IAPVRAAAAATEAVRGLADRLVRAGLAVPAGAGTTVAAGVRRVRFAALAVVALGVTALL
                     LPVEPGMPRHLVAFWFALPLALTLSCLALARIEAHPYSHWASPAGQRLLGRLARHSADA
                     DGDRAYLTTVAVRGVGAVGEPELRAAFARRGPVRRG"
     gene            7364..8950
                     /locus_tag="AMK31_RS05350"
                     /old_locus_tag="AMK31_05250"
     CDS             7364..8950
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (110-484). E-value:
                     2.2e-20. Score: 65.2. Matches aSDomain:
                     nrpspksdomains_AMK31_RS05350_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432060.1"
                     /locus_tag="AMK31_RS05350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05250"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_073939204.1"
                     /sec_met_domain="PF00561 (E-value: 8.1e-26, bitscore: 86.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYTAAGSLLLTTLAAAPADSAPKTPVAAAEVRGTAVAVAR
                     AAAKGVRFGRCTAGRDMPASMECGTVTVPLDYAQPDGRQIQLAVSRVPATERDPQDGER
                     QVTRQGALVYNPGGPGASGYHFPLVGMLPKWKRIAAAYDLVGYAPRGVGRSAPLSCQDP
                     AQVFKGPTQSPAHPSESYKRDRIAKAKAYARGCLERSGDALRHYHSLNNARDLDVLRAA
                     LGEDRLNFIGSSYGTYFGALYATLFPSHVRRMVFDSAVNPHPSDVWYRNNLAQSAAFED
                     RWADFKDWIAKHHDVYGLGARAQDVQQGYDLARARLAAEPAGGRVGSGQLQSLFLQAGY
                     HDDHWPARARALSAYLKGDPQPLIDLAGPRRKASPAAENSSAVYTAVECNDAPWPTDWA
                     VWDRDNTRLARVAPFETWDNVWMNLPCAYWPAPRQQPLDVRTGPGELPPTLILAAERDA
                     AAPYEGALELHRRLSGSVLVTERDAGTHGLVGGPNACVNGYVDAYLMEGWVPGRRAACA
                     PRPEPEPGTGR"
     aSDomain        7694..8815
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK31_RS05350_Abhydrolase_1.1"
                     /evalue="2.20E-20"
                     /label="AMK31_RS05350_Abhydrolase_1.1"
                     /locus_tag="AMK31_RS05350"
                     /protein_end="484"
                     /protein_start="110"
                     /score="65.2"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASGYHFPLVGMLPKWKRIAAAYDLVGYAPRGVGRS
                     APLSCQDPAQVFKGPTQSPAHPSESYKRDRIAKAKAYARGCLERSGDALRHYHSLNNAR
                     DLDVLRAALGEDRLNFIGSSYGTYFGALYATLFPSHVRRMVFDSAVNPHPSDVWYRNNL
                     AQSAAFEDRWADFKDWIAKHHDVYGLGARAQDVQQGYDLARARLAAEPAGGRVGSGQLQ
                     SLFLQAGYHDDHWPARARALSAYLKGDPQPLIDLAGPRRKASPAAENSSAVYTAVECND
                     APWPTDWAVWDRDNTRLARVAPFETWDNVWMNLPCAYWPAPRQQPLDVRTGPGELPPTL
                     ILAAERDAAAPYEGALELHRRLSGSVLVTERDAG"
     gene            complement(9000..9725)
                     /gene="hemQ"
                     /locus_tag="AMK31_RS05355"
                     /old_locus_tag="AMK31_05255"
     CDS             complement(9000..9725)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="AMK31_RS05355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05255"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_073939205.1"
                     /transl_table=11
                     /translation="MSDDATTTGRVPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDRAG
                     YADEVQELFDQLAAKDVTIRGTYDVSGLRADADVMIWWHAETSDQLQEAYNLFRRTRLG
                     RALEPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRRML
                     ADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVREE
                     VPFFTGRRKSVADLVAGLA"
     gene            complement(9730..11184)
                     /gene="hemG"
                     /locus_tag="AMK31_RS05360"
                     /old_locus_tag="AMK31_05260"
     CDS             complement(9730..11184)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981981.1"
                     /locus_tag="AMK31_RS05360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_05260"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_073939206.1"
                     /sec_met_domain="DAO (E-value: 1.4e-11, bitscore: 39.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGVRSDAGRVVVIGAGIAGLAAAHRLLERGARVTVLEASDRVGG
                     KLLPGEIAGVRVDLGAESLLARRPEAVGLAREAGLADRLEPPATATAALWTRGALRPMP
                     KGHVMGVPGTAAALAGVLSEEGLRRIERDAELPRTEVGDDVAVGEYVAARLGREVVDRL
                     VEPLLGGVYAGDAYRISLRSAVPQLFQAAHTHDSLTEAVREIQAKAAANPLTGPVFMGL
                     RGGVGTLPLAVADSVRARGGEILTRTAATELRRRPGGGWLVTAGDRVLPADAVVVAVPA
                     PAAAGLLRAEAPAAAAELTAVEYASMALITLAYRRADIRLPEGSGFLVPPVDGRTIKAS
                     TFASQKWGWIAEEDPETVVLRTSVGRHGETEILDRDDAGLVAVSRQDLAAATGLDATPV
                     ATRVTRWDGGLPQYPVGHHARVARIRGHVAGLPGLAVCGAVYDGVGVPACIASAYAAVD
                     QLGGDLRGVQELTAHPVQSLHGGAGE"
ORIGIN
        1 ggccggcgcg gccgcgtcca cctggtccgc aaccacgaga accgcgccac cgccagactc
       61 ggcgtcccca cggtcgacgg cctgacctac gacccggcgg gcaagggcgg ctgtacggcg
      121 ctcactctcg acgcccgcaa cgaggtgctg tccgagcggg tcgccatcgc cggcacggcc
      181 gtcaactgct cgggcgggcc gaccccctgg ggcacctggc tgacctgcga ggagaccgag
      241 gacaaggcgg gcacgaacgg ctacgccaag gaccacgggt tcatcttcga ggtcgacccg
      301 gccgacccgc gccgcaccgg ggcggtcccg ctgaccgcga tgggccgctt ccagcacgag
      361 gcggtggccg tcgacccgcg cagcggcatc gtgtacgaga ccgaggacgc gttcatccag
      421 cccttcggcc tcttctaccg cttcctgccg cgcaagccgc tcggcggcac cggcgccctg
      481 cgcgcgggcg gcagcctcca ggccatgcgg gtccccggcg tgccggacct gtcggccgtg
      541 caggaacccg gcgcctcctt cgacggcgtc gagtgggtgg acgttccgga cccgctggcc
      601 gcgaagacgc cggtccgcct ccaggactac ggcaagaagg gcgtcacccg cgcgcagaag
      661 ctggagggct gctactgggg cgggcgcagc gtgtacttcg tgtcgtcgta cgcccgcagc
      721 cgggaggggt cggccgccga ccacttcggg cagatctggc gctacgaccc cgaccgccgc
      781 cgtctcaccc tggtgatcgt cttcggcccg gacacggacg tccagctgcc cggcgagtcc
      841 ccggacaaca tctgcctcgc gcccagcggc ggcctgatgg tcagcgagga cggcaacggg
      901 gcccagcacg tcttcggcgt cacccggaag ggggaggtgt acgagatggc ccgcggccgg
      961 cagaacatcg gcaccgacga cgagccggag tggggcgagt tcgcgggcgt caccttctcc
     1021 cccgacggcc ggaccatgta cgtcaactgc tacacgcccg gtacgacgtt cgcggtgacg
     1081 ggcccctggc gcaagtagcg acggcacccg gccgcccggc cgggtgagtt ccggcgggcg
     1141 ccgagcgcac cgacggcgac gcgggccgat tcctcccctt cgcccgctta cgttcgtatg
     1201 atgatcgttc ttgtacgacg tattgtcgcc gtctgtgcgc tcggcgccgc actctccgcg
     1261 tgcggcgcgc ccgaggccgc ccgtaccgcc tcccgggccg cccctccggc ctcagcctcc
     1321 gcttccccgg cctccccctc ccccggctct ccctcccccg cccggccacc caccctcgcg
     1381 cccggccccg gcggcatgac gccggtgttc cacaacgggc cccggacgcc cggcgacaag
     1441 accgtcgcgc tcaccttcga cgccgacatg accgccgacc aggggccgcg agccgccatg
     1501 ggcgagcggt tcgacaatcc gcggctgatc gccacgctgc gggcgctgag ggtgccggcc
     1561 accgtgttca tgacgggccg gtgggccgag gagtatccgg tccaggcccg ttccatcggc
     1621 cgggacccgc tgttcgaggt cgccaaccac tcctacagcc actacgccta caccgaggac
     1681 tgctacggcc tgcccacggt cgacgaaccg ctgatgcgcg cggacgtgga gcgggcctac
     1741 gccgccttcc gcgcggtggg cgtcccggac gccaggccgt acttccgctt ccccggcggc
     1801 tgttacgacc ggcgggccct gcggacgctc gccggtaccg gggtgaccgc cgtgcagtgg
     1861 gacgtggtgg gcggggacgc cttcgcgacg gacgccgacg cggtggcccg gcaggtactg
     1921 gacggcgtcc ggcccggatc ggtggtcgtc ctgcactgca cgacgagcgc cgcccccgcg
     1981 accgaacggg cgatccgcac gatcgtgccc gagctgcgcc accggggttt ccggttcgtg
     2041 aaggtgtccg aactgatcag tgccgcaccc cgctgaccag gcctaccctg gagggtatga
     2101 gcgaggccga ggtcaacgac tactgcctga tcgacgcgac caggccgccc aaggcggacg
     2161 ggccgccgta cgcggagtgc gtgctgtgcc gggagccgac ggagtacgcc gagtcgtaca
     2221 aggggatcac cctgtgcccc gtctgcgagt ggcaggaggc acagcgcacc gcctgctccg
     2281 gctgaccggc ggggacgggc gtctcccgcg gatcttccgc ctggcagact gaaggacgtg
     2341 acacatcctt cctccgttcc cggcgacagt cccttccgtc ccgtccgctc ggcccgtgac
     2401 gaggccccgc agttcgttct cccgctggtc gtgcgcatcg agcgggccga gcccccggcg
     2461 cgcaccgacg cgctggagac ggccgccaag gcggtgctcg tgatgctgtc cgacgagcgg
     2521 tcgctaaggg acggcgagtg ggcgcaggcc gtgcgggact ggcaggacgc ccggatccgc
     2581 aaggtcgtcc gccgggcccg cggcgcggag tggcggcgag cggagacgct gcccgggatc
     2641 acggtcaccg ggaagtcggc ggaggtgcgg gtcttcccac cggtgccgct ggacggctgg
     2701 ccgaaggaac tggccaagct ccaggtgtcc ggcaccgacc tcgacgaccc ggagcggccg
     2761 accgcggccg acccgggcac gccggtgctg tggatgagcc cggaggtgga gatgtcggcc
     2821 ggcaaggcca tggcgcaggc cgggcacggc gcccaactgg cctggtgggc gctgccggac
     2881 gaggagcgcg cggcctggcg ggacgccggt ttcccgctcg ccgtgcgcac cgcggacccg
     2941 gcgcgctggc gcgcgctgac cgcgagcggc ctgccggtgg tgcgggacgc cgggttcacg
     3001 gagatcgcgc ccggctcctg cacggtggtc gcccagcatc cggcgctgcg ttagcgggtg
     3061 tgcgcgggct cggccatgtc acgagcaggc cgcgggctcg ccgtcgtgcg gtacggacat
     3121 ggccggcgcg cctgccggca gcctttcagg gcccccgggg gccgcgggtt ccgcgcggtc
     3181 agaagcgtcc gccggtcagc cgcgtgatcg gcccgtgccc gcggcgcgcg gagacccggc
     3241 ccgccgcgaa ggacagggtg gcgagggccg tcacacccgt cgcggcaccg gtcgcgatca
     3301 gcttgcgggc cttgatgacc gtccatgccg tcgtggcggc ggtggccacc tgaccggcgg
     3361 tcgacaccac ggtgtccttg gtggtcgcga gcgcggtgcc cgcggccgcc accgcccgcg
     3421 atccgccctc ggacgccgaa cgcgcggccc gcgcggcggt ggtgcccgac gaggcggcct
     3481 tgtccccggt cttcgacgcg gccgtcttcg ccgtacgggc ggcctgcgcg gtcttgcctt
     3541 ctcgctcttg tgtgttcgcc atggagagcg cgttgccgat tccgctccgg acaaacgcac
     3601 tacgtccacg ggctcttggg caacccccgt ccgtccgcgc tgcgtccgac ggcgtcgcgc
     3661 gggtagacgc agcgaagagg aggcctacag cttctcaccg cacagcgacc atggaggtgt
     3721 catgcccggc atcatcaacc ggatcaagca gtacagccgc acccctcagg gccggcgggc
     3781 catcgcgtcg gcgcggcgcg cggcctcgga cccccgtacc cgggcgcagg cccgcaccct
     3841 gctcggccgg ctgcgccgcc gctgaccgcg ccggcccgcg agcaggtgcc ggcttccctg
     3901 cccggggcgg acgcatatcc cgtcccgccc cgggcatccg ggccttcgcc ggcgtcgaac
     3961 accgccgggc ggatccacga cagaagggtg gcggccatgg cgaccggagc atccgtgacg
     4021 cgaggcaagg gggccgcgag ggcccgccgg gcggcgaacg gttccgtcgt cgacgccggg
     4081 gcccgctggg gcctggtggc ccgcggcacg atctacctgc tgatagggct gctggccctg
     4141 cgcatcgccc atggcggcgg cgccaggcag gcggacagca gcggagccgt ccaggagctg
     4201 gcgcggcagc cgttcggtga ggcgctggtg tgggcgatcg ggatcggcct gatcgggatg
     4261 gcgctgtggc ggttgtccga ggccctgttc ggagcctccg gcccggacgg ccgcaaggcg
     4321 tccaagcgcg ccctgaacgc cgggcgctgc gtcttctacg gcttcgtggc gtcctccgtg
     4381 ctgtcgttcg tgacctcctc caggaacgac gagggaagcg acgcgacctc acgcgacctc
     4441 accgcccgcg ctctcgaact ccccctgggc cgctggctcg tcggcgtcgc gggcctcgtc
     4501 gtcctggggg tcggcgtggg catcggggtg cgcgcggtcc ggcgcacctt ccacaagcac
     4561 ctcaagcggg cggacatgtc ccgccgggtc cgccgcgtgg tggacgtcct cggcgtcacc
     4621 ggcggtgtgg cccgcgggct ggtctacgcg gcggcgggcg tcttcgtggt gatcgccgcg
     4681 gtggactacg accccgaccg ggccaagggc ctggacggca cgctgcgcgc tctcgccgac
     4741 gccccggccg ggccctggct gctgtccctg gtggcgctcg gtctggccgt cttcggtctg
     4801 ttctccgtcg ctctcgcccg ctaccgcagg gtctgaggcg ggcggcgccg ggcctctcgg
     4861 gttctccgcg acgggcgggg ccggaacctc aaatgttgct ctgaaggcgc ggcccgcagg
     4921 gttcgggccc cggcggtgtg gccatacccc cctcacgcgg tgtgcggaac gccgtgcgag
     4981 aacgtggagg tgggggaagc gtgcagcgac tggggacggg gatcgggtgg cggccggaga
     5041 tcgcggacgc cgtggaggcg atgccgggca tcgactgggt cgaggtcgtc gccgagaacg
     5101 tctgcctcgg ccatctgccg gactcgctgc ggcggttgcg cgagcgcggg gtgaccgtgg
     5161 tgccgcacgg cgtcgcgctc ggcctcggcg gcgccgaccg gccggatccg ggccggctgt
     5221 cggcgctggc cgagcgggcc gaggcgctgg ggtcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtccgcgc gggcggaccg ctgaccgcgt caccccggct ggaggcgggc cacctgctgc
     5341 cggtgccgcg cacccgggac gcgctcgacg tgctgtgcga gaacgtgcgg atcgcgcagg
     5401 acgcgctgcc ggtgccgctg gcggtggaga acatcgccgc gctgttctcg tggccgggtg
     5461 aggagatgac ggagggacag ttcctgtacg agctggccga ccgtaccggc gtacgcctgc
     5521 tgatcgacgt ggccaacctg cacaccaacc acgtcaaccg gggcgaggac ccggccaagg
     5581 cgctggccga actccccgtg gaggccatcg cgtacgtcca tgtcgcgggc ggcttcgaac
     5641 gtgacggcgt ctggcacgac agccacgccc accccgtgcc ccggccggtc ctggacatcc
     5701 tgaccgacct ggcgtcgcgg gtggcaccgg ccggggtgct gctggagcgg gacgagaact
     5761 tccccgaacc ggccgagctg gagggcgagc tggcggccat ccgggaggcc ctgcgcaagg
     5821 gccgggggca gtcgcgggcg gccggggcgg cgacggcggg gacctccgcg ccgcgcccgg
     5881 cggccaccgc cgcgaccgcc gtcccggacg gcgtgcgcga acggctcggt ctcgcccagg
     5941 ccgcgctgct ctccgcgctc gtcgccggga cacccgcccc ggaggggttc gaccgggccc
     6001 ggctgcgggt gcagacgcgg gcgctcgccg ggaagcgggc gggtgtcgtg gcgaaggtgg
     6061 ccccggaact gccggatctg ctcggggacg cgtaccggac ggagttcctc gcgtacgcgc
     6121 accggcgtcc gatgaccggc ggctaccggc gggacgccct ggacttcgcc gagcacctgc
     6181 tggactcggg ccgtctgcag gcgcgggtac ggcgggagat gcgggcgtgg tggcgggagc
     6241 gctcgggccc ggcgccgcgt tcggcacgtc cggcggcacg gctggcgcgc ggcgcccgcc
     6301 gggtgctgct ggggcgctga gcgacgggag ccgccggcct gcccggctcg tactgcggaa
     6361 cgtcacatac ccgcgacact ctgtccggat gatgaacagg ggatggcgct cgacgcgatc
     6421 accggcatag aaacgcggca tgttctgggt catcctcctg ctgctcgcct gggtcgccgc
     6481 cggtacggcg tgcaccttgc tgtgtctggc cgccgtgcac gccgccggca ccgacgcgga
     6541 cacgggcgga acgcacgagc tgacgctcta cgaggccgcg ttcctctccg gcgggcccgc
     6601 gcgggtcgcc gaactgacgc tggtctcgat ggcccgccag cgccggctgc tgctcgcgca
     6661 caccggctgg gcgaccgtcg tcgacccgcg cgggcgcgac gagatggagc gttcggtgat
     6721 aggggccatc ggcccggagg ggcagtcccc gatcgccccg gtgcgggccg ccgcggccgc
     6781 caccgaggcg gtgcgcgggc tcgccgaccg gctggtccgc gcgggtctcg ccgtcccggc
     6841 cggggccggt acgacggtcg cggcgggcgt gcggcgggtg cggttcgccg cgctggccgt
     6901 ggtcgcgctg ggggtgacgg cgctgctgct gccggtcgag ccgggcatgc cgcgccacct
     6961 ggtcgccttc tggttcgcgc tgcccctcgc cctcacgctg agctgcctcg ccctggcccg
     7021 catagaggcg cacccgtact cgcactgggc ctccccggcc gggcagcggc tgctgggccg
     7081 gctggcccgg cactccgctg acgcggacgg cgaccgcgcc tacctcacca cggtcgccgt
     7141 ccgcggggtc ggcgcggtcg gcgagcccga gctgcgggcg gcattcgccc ggcgagggcc
     7201 cgtccggcgc ggctgacggc gggccgcgcc cgcgcccggg gagacccatt cagggggcgt
     7261 acggaagcac gggcgccgac accgggcggg cggtgcttgc cttcgccaag aaccaaacga
     7321 aacttccctt ttgtcgctgc cgcgcaccga aagggagtac gcgatgagag ctgccgccct
     7381 ctacacggcc gccgggtccc tgctgctgac caccctcgcc gccgctcccg ccgacagcgc
     7441 gcccaagacc ccggtggccg ccgccgaggt acgcggcacc gcggtggccg tcgcccgggc
     7501 cgcggccaaa ggggtccggt tcggccggtg cacggcgggg cgggacatgc cggccagcat
     7561 ggagtgcggc accgtgaccg ttccgctcga ctacgcgcag ccggacggca gacagatcca
     7621 gctcgccgtc agccgggtgc ccgccaccga gcgcgacccg caggacggcg agcgccaggt
     7681 gacccggcag ggcgccctgg tctacaaccc gggtggcccc ggcgcctccg gctaccactt
     7741 cccgctggtc ggcatgcttc ccaagtggaa gcggatcgcc gccgcctacg acctggtcgg
     7801 ctacgccccg cgcggtgtcg gccgttcggc gccgctgtcc tgccaggacc ccgcgcaggt
     7861 cttcaagggc cccacccagt ccccggcgca cccctcggag tcgtacaagc gcgaccgcat
     7921 cgcgaaggcg aaggcatacg cccgcggctg cctggagcgc tccggcgacg cgctgcgcca
     7981 ctaccactcc ctcaacaacg cccgcgacct cgacgtactg cgcgcggcgc tgggcgagga
     8041 ccggctcaac ttcatcggct cgtcgtacgg cacctacttc ggggcgctgt acgcgacgct
     8101 gttcccctcg catgtgcggc ggatggtgtt cgactcggcg gtgaacccgc acccgtcgga
     8161 cgtctggtac cgcaacaacc tcgcccagtc ggccgcgttc gaggaccgct gggcggattt
     8221 caaggactgg atcgccaagc accacgacgt gtacgggctg ggcgcccggg cgcaggacgt
     8281 gcagcagggc tacgacctgg cgcgggcgcg gctcgccgcg gagccggccg gcgggcgggt
     8341 cgggtcgggg cagttgcaga gcctgttcct ccaggccggg taccacgacg accactggcc
     8401 ggcgcgagcg cgggcgctgt cggcgtatct gaagggcgat ccgcagccgc tgatcgacct
     8461 ggccggtccg cgccggaagg cttcgcccgc ggcggagaac tcgagcgcgg tctacacggc
     8521 cgtggagtgc aacgacgcgc cctggccgac ggactgggcg gtgtgggacc gggacaacac
     8581 ccggctggcg cgggtggcgc cgttcgagac gtgggacaac gtgtggatga acctgccctg
     8641 cgcgtactgg cccgcgccgc gtcagcagcc gctggacgtg cggacggggc ccggggagct
     8701 gccgccgacg ctgatcctgg cggcggagcg ggacgccgcc gcgccgtacg agggggcgct
     8761 ggagctgcac cggcggctgt cggggtcggt gctggtgacg gagcgggacg cggggacgca
     8821 cgggctcgtg ggtgggccca acgcctgcgt caacgggtac gtcgacgcgt atctgatgga
     8881 ggggtgggtt ccggggcggc gggcggcgtg cgcgccacgc ccggaacccg agccggggac
     8941 cggccggtag gaccgccggc ccgtccgcgg ggcggtgcgc ggccccgcgc cgccgggcgt
     9001 caggccagtc cggcgaccag gtcggcgacc gacttgcggc ggcccgtgaa gaacgggacc
     9061 tcctcgcgga cgtgcatccg ggcctccgag gcgcgcaggt ggcgcatgag gtcgacgatg
     9121 cggtacagct cgtcggcctc gaaggcgagg atccactcgt agtcgccgag cgagaacgag
     9181 gcgaccgtgt tggcgcggac gtccgggtag ccgcgggcca tcttgccgtg gtcggcgagc
     9241 atccggcggc ggtcctcgtc gggcagcagg taccagtcgt aggagcgcac gaacgggtag
     9301 acgctgacgt agtcgcgcgg cgtctcgtcg gcgaggaacg ccgggatgtg tgagcggttg
     9361 aactcggcgg ggcggtgcag cgccatgttg gaccacaccg gctccagggc acggcccagc
     9421 ctggtgcggc ggaagaggtt gtacgcctcc tggagctggt cgctggtctc ggcgtgccac
     9481 cagatcatca cgtcggcgtc ggcgcgcagg cccgagacgt cgtaggtgcc gcggatggtc
     9541 acgtccttcg cggcgagctg gtcgaacagc tcctggacct cgtcggcgta gcccgcgcgg
     9601 tcctcgggga gcacgtcctt cagcttgaac acggaccaca gggtgtagcg gatgacctcg
     9661 ttgaggtcct tggccagctt gcccttgttc gggacccgtc cggtggtggt ggcgtcgtca
     9721 ctcatgccct cattctcccg ctccgccgtg caggctctgc accgggtggg cggtgagctc
     9781 ctgcacaccg cgcaggtcac cgcccagctg gtccaccgcg gcgtacgcgc tcgcgatgca
     9841 ggcgggaacg ccgacaccgt cgtacaccgc gccgcacacg gccagtcccg gcagtccggc
     9901 gacgtggccg cggatgcggg ccacgcgcgc gtggtgcccg accgggtact gcggcaggcc
     9961 gccgtcccag cgggtgacgc gggtggcgac cggcgtggcg tcgaggccgg tggccgccgc
    10021 gaggtcctgc cgggagacgg cgacgaggcc ggcgtcgtcg cggtccagga tctccgtctc
    10081 gccgtggcgt cccacggagg tgcgcagcac caccgtctcc gggtcctcct cggcgatcca
    10141 gccccacttc tgcgaggcga acgtggacgc cttgatggtc cgtccgtcga ccggcgggac
    10201 caggaagccg ctgccctcgg ggaggcggat gtcggcgcgc cggtaggcga gggtgatcag
    10261 cgccatcgac gcgtactcca cggccgtcag ctcggccgcg gcggcggggg cctcggcgcg
    10321 cagcagtccg gccgcggccg gcgcggggac ggcgacgacc acggcgtccg cgggcagcac
    10381 ccggtcgccg gccgtgacga gccagccgcc gccgggccgg cggcgcagct ccgtggccgc
    10441 cgtccgggtc aggatctccc cgccgcgcgc ccgcacggag tcggccaccg ccaggggcag
    10501 ggtgccgacg ccgccccgca ggcccatgaa caccgggccg gtcagcgggt tcgccgccgc
    10561 cttggcctgg atctcgcgga ccgcctcggt gagcgagtcg tgggtgtggg cggcctggaa
    10621 gagctggggg acggcggagc gcagggagat gcggtacgcg tcgcccgcgt agaccccgcc
    10681 gagcaggggt tcgacgaggc ggtcgacgac ctcgcggccc agcctggcgg cgacgtactc
    10741 ccccaccgcc acgtcgtcgc cgacctcggt gcggggcagt tcggcgtccc gctcgatgcg
    10801 gcgcaggccc tcctcggaga gcactccggc cagggcggcg gcggtgccgg gcacgcccat
    10861 gacgtggccc ttgggcatgg ggcgcagggc gccgcgggtc cagagcgcgg cggtcgcggt
    10921 ggccggcggt tcgagccggt cggcgaggcc ggcctcccgc gcgaggccga ccgcctcggg
    10981 gcggcgcgcc agcagggact cggcgccgag gtcgacccgc acgcccgcga tctcgccggg
    11041 cagcagcttg ccgccgaccc ggtcggacgc ctccagcacg gtcacccgcg cgcctcgctc
    11101 cagcagccgg tgggcggcgg ccagcccggc gatcccggct ccgatgacga cgacccgccc
    11161 ggcgtcgctc cgtactccgc tcatgccctc caccttctca gacggcaccg acggcgccct
    11221 cgcggggccg tggcccggcc gagtcccgac cgtgaccggt tcggaaccgg tgccggcaaa
    11281 cgcccggcgc cggccggacg tcgaaggaac gtccgtcgca
//
