LOCUS       NZ_LIVZ01000001        10606 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0107 scaffold1, whole genome shotgun sequence.
ACCESSION   NZ_LIVZ01000001
VERSION     NZ_LIVZ01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0107
  ORGANISM  Streptomyces sp. TSRI0107
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 934909)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934909)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905845.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,745
            CDSs (total)                      :: 7,667
            Genes (coding)                    :: 7,467
            CDSs (with protein)               :: 7,467
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 85 of 200
            Pseudo Genes (incomplete)         :: 158 of 200
            Pseudo Genes (internal stop)      :: 13 of 200
            Pseudo Genes (multiple problems)  :: 51 of 200
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:19:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 351721
            Orig. end                         :: 362327
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVZ01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10606
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10606
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10606
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5606](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5606
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    796..810
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.07"
                     /tool="antismash"
     gene            981..1220
                     /locus_tag="AMK31_RS01550"
                     /old_locus_tag="AMK31_01510"
     CDS             981..1220
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="AMK31_RS01550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01510"
                     /product="chaplin"
                     /protein_id="WP_073938432.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSAAVAAVAGTAGVAAADSGAHGAAAHSPGVLSGN
                     AVQIPIHIPINLCGNTVDVIGLLNPAFGNNCLNH"
     gene            1465..2361
                     /locus_tag="AMK31_RS01555"
                     /old_locus_tag="AMK31_01515"
     CDS             1465..2361
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529517.1"
                     /locus_tag="AMK31_RS01555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01515"
                     /product="hypothetical protein"
                     /protein_id="WP_241842500.1"
                     /transl_table=11
                     /translation="MRGLPVRRIASTALCATLVLGIAGPAAMAADHDPARERTPATSQA
                     PVADAKALLGQTQTLNDLGVVLDPVTKMLDAVLKADEGQLSAEEAKKLGDAAKASIAKL
                     GTAASAAPAAPAAPAVPAVPAAPSAPTVPSTSNAPIVTLPAPASLVKNDPAASATDVRA
                     GVLDDLQKAIDALIEAATSGLVDQVVPAATAVVTGLVNVLTTTLVGGALPAHTVAGLPP
                     LPSLPAAEASAPQSPAALPEEPSAAAEAAPAQPQSPVALPEEPSAVSEAAPAQPELTPA
                     LPEAPQTVPTVGLFMTP"
     gene            2597..2887
                     /locus_tag="AMK31_RS01560"
                     /old_locus_tag="AMK31_01520"
     CDS             2597..2887
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116485.1"
                     /locus_tag="AMK31_RS01560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01520"
                     /product="hypothetical protein"
                     /protein_id="WP_073938433.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSMVIAGAAAPAFAHDTADLTPTSLNGAVNTLTN
                     RPIEPMDVMPLKQQSNALDTENKDSVLSTVNGVTKTLNAKGGLLGGLPLRG"
     gene            3083..3580
                     /locus_tag="AMK31_RS01565"
                     /old_locus_tag="AMK31_01525"
     CDS             3083..3580
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116484.1"
                     /locus_tag="AMK31_RS01565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01525"
                     /product="DUF5949 family protein"
                     /protein_id="WP_073938434.1"
                     /transl_table=11
                     /translation="MTSTPNETRPFRAMDMGTLVVMAWSGEAPGGDMPYLLAYTLGDGP
                     DGPEAASAAVARMLNDNGLPVGGDLVDGASKPGLPVGLLVEAGQAVVNMPHLNAQCTPP
                     PEWLAAVGERGFAYFVFTTRPWPEAQPGKPVDPEALAAFAGADETLDAAAHVVLPVRSL
                     RS"
     gene            3580..4197
                     /locus_tag="AMK31_RS01570"
                     /old_locus_tag="AMK31_01530"
     CDS             3580..4197
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116483.1"
                     /locus_tag="AMK31_RS01570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01530"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_073938435.1"
                     /transl_table=11
                     /translation="MAAAAGRHAHPAVPAVGGGRGFALLLVLTGAAGLLASWVITLDKF
                     KLLEDPDFTPACSLNPVVSCGSVMESDQASAFGFPNPMLGLAAYAVVVCVGMSLLAGAA
                     FPRWYWLTFDAGCLFGVGFVSWLQFESLYRINALCLWCALAWLATVVLFWYVTSFAVRH
                     GFLPAPDGLRSFLTDFTWVLPLAHLGVIGMLILTRWWDFWTS"
     gene            4326..4841
                     /locus_tag="AMK31_RS01575"
                     /old_locus_tag="AMK31_01535"
     CDS             4326..4841
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="AMK31_RS01575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01535"
                     /product="hypothetical protein"
                     /protein_id="WP_073938436.1"
                     /transl_table=11
                     /translation="MKPTTRGTLAAVVTGVAAAVGAAASPAVAADTVPVPVPLNGAEKS
                     LGMELPEIGAELPLPKPGSPEGPRYVEGRLVPDRAVPQLPVRGGLPGADVRAPLPRLLG
                     DDFDHVGVHAPASDLRTLGPGLAVDAPLTPPNPDNYGLPGLKPPQAGVVTPVLQTVPGA
                     ELGTGPGL"
     gene            5001..5606
                     /locus_tag="AMK31_RS01580"
                     /old_locus_tag="AMK31_01540"
     CDS             5001..5606
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065674.1"
                     /locus_tag="AMK31_RS01580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01540"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_073938938.1"
                     /sec_met_domain="melC (E-value: 5.6e-47, bitscore: 155.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVSVGAAAVNMENEPGRAAGTPDPPAGTPDPPAGRGTRRDLARG
                     MFAAAVALTIAPLVVASRPARREDPQNPQDPQNPQDPPADVAFDEIYRGRRIRGLRAPG
                     AVPASAAGRVFGTDGWHVTVDGHPLHLMRRADGGWLTMVDHYRSYPTPLEAARAAVDEL
                     GPGQSLGTPDADTASGEGTHEGTHEGTRGQGGHHGVHP"
     gene            5590..6456
                     /locus_tag="AMK31_RS01585"
                     /old_locus_tag="AMK31_01545"
     CDS             5590..6456
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="AMK31_RS01585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01545"
                     /product="tyrosinase family protein"
                     /protein_id="WP_073938437.1"
                     /transl_table=11
                     /translation="MVYTRKDVSTLTATERRRFVKALLELKRRGEYDEFVRTHIEYYVA
                     DGENGLRTAHMAPSFLPWHRQFLLELEKALRRIDSSVSVPYWDWTRDRTTTSVPWTKDL
                     LGGNGRPSDRQVTTGPFAYAEGNWTLKVGVTNGKFLTRDLGRARDPLQLPTAGELQWAL
                     KDPVYDVAPWDSTVTQGFRNKLEGWGTGRGSASWRNHNLVHRWVGGAMVSGASVNDPVF
                     WLHHAFVDLVWSRWQQRHRDDRYRPVKQPAAGDPQRGRVVARHEKLPPWDVTPDELDDH
                     GSIYRYA"
     gene            complement(6544..6867)
                     /locus_tag="AMK31_RS01590"
                     /old_locus_tag="AMK31_01550"
     CDS             complement(6544..6867)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672799.1"
                     /locus_tag="AMK31_RS01590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01550"
                     /product="chaplin"
                     /protein_id="WP_073938438.1"
                     /transl_table=11
                     /translation="MSRITKAAGVALGTGAVVLGGAGLAVADAGAQGAAVHSPGAVSGN
                     VAQVPLNIPINLCGNTVDVIGLLNPAFGNTCLNHSDHKGKGHDDGKGHDDGKGHGGGHG
                     YGH"
     gene            complement(7117..7341)
                     /locus_tag="AMK31_RS01595"
                     /old_locus_tag="AMK31_01555"
     CDS             complement(7117..7341)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016825815.1"
                     /locus_tag="AMK31_RS01595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01555"
                     /product="cold shock domain-containing protein"
                     /protein_id="WP_073938439.1"
                     /transl_table=11
                     /translation="MVTATVREWYDEEGWGVLDSPETPGGCFGHYSDIQAPGFRTLSPG
                     QRVDLTWEAPGFKQDGYDYRAVSIVPRPA"
     tRNA            complement(7496..7571)
                     /anticodon=(pos:complement(359257..359259),aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="AMK31_RS01600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="AMK31_01560"
                     /product="tRNA-Arg"
     gene            complement(7496..7571)
                     /locus_tag="AMK31_RS01600"
                     /old_locus_tag="AMK31_01560"
     misc_feature    7788..7803
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    7853..7867
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.53"
                     /tool="antismash"
     misc_feature    7862..7876
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.08"
                     /tool="antismash"
     misc_feature    7865..7881
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.17"
                     /tool="antismash"
     misc_feature    complement(7874..7892)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.4"
                     /tool="antismash"
     gene            7892..9496
                     /locus_tag="AMK31_RS01605"
                     /old_locus_tag="AMK31_01565"
     CDS             7892..9496
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435092.1"
                     /locus_tag="AMK31_RS01605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK31_01565"
                     /product="dynamin family protein"
                     /protein_id="WP_073938440.1"
                     /transl_table=11
                     /translation="MVTLDVRPQLLDALSTLRDRVAAARFPLPLSGAPRARANRDELLA
                     QLDDYLVPRLRQPEAPVLAVVGGSTGAGKSTLVNSLVGRRVSEAGVLRPTTRTPVLVCN
                     PEDHHWFSGMRVLPDLTRVWVPQQESGDELLLPGENPTRVLRIETTETLPPGLALLDAP
                     DIDSLVADNRVLAAELICAADIWIMVTTAARYADAVPWHLLRTAKEYDVTLVTVLDRVP
                     HQVVSEVSRQYGALLTKAGLGHVPRFTVPELPESAGGGGLLPVTAVAPLRTWLIHQAQD
                     PAARQHTLARTAHGVLNSLKSRMPELASAAAAQYAAALRLTAAVDTAYDSEYARVRGRL
                     QAGAVLAGDALKRWRAFPLDCTPKELLDALVESLGDLLLCAVTAADERVDDAWRLEPAA
                     GAPELADDAEPHHAGHRIGLAVRRWRRELEEYAEEEVRHLERSVAPDPEMIAALVATVL
                     LGGRRARSAGEGLAERIGAHGALRLRDRGGRLLAEHLDRVIQTERERRLAPLDALDVHP
                     EPQAALIAALSVLQKER"
ORIGIN
        1 ccctggtccg ctcgtacagt tgccgggcct gccgaggggt gtcgaccagg acggtgctgc
       61 gcaggccggt cccgggcggg agcagccagg ggcggctcca cttcgccacc accggcagtc
      121 ccaggcagcc ggcggcggcc gccgcctggg cagcgctgtc cgggaccacg gtcacggggt
      181 gcgggacgtc cagggcggtg cacaccccgg ccagttcggt cttgtcggcg acgtgctcgg
      241 gcagcaggtc ggagggggcg ggcagcagat agccggcggc gagttccgtg cgcagggcgc
      301 ccaccgcgac ggcggtcgcg tcgtccatgg ggagcaggac ggcgggacgt cccacctcgg
      361 cggcgacccg gcgcagcgtg gcggcgacgc cgtcggagcc ggcgtgcggc gcgggcggcg
      421 gatgcagccg ccgtacgaag cgggaccggc cgacggggct tcccgcgcgg tcggcgacca
      481 ggtggacctc caccccggcc cggccgaggg tgcgcacggc gcccagggtt ccgtgatgga
      541 acggattccg gtcggtccgc agcagaacgg cggggacgcg ggtgtcgaac ggcgacacag
      601 tggctctttc cttcggtggt ttgctcacgg gtctcactgg tgcgggcgag ggcgcgtcgc
      661 gcccgccccg gcggctccgt ccgtctcccg gccgcccgcg cccacggcac ccgtggtgga
      721 cgcccccgcg gcccaggccg cgtccggctg gggaacgagc cgggaggccc gctggtcacc
      781 accgttcccc aaagcatcac cgagatcgat cgctatccgt accaataagg agaaaccagt
      841 caaggtagtc cgaagcgtga aaccacgaca cccgggagat cactccttcg gaggcacaac
      901 cccggcgcgt cccgcgcgtt gacacagcgc cgggcatccc gcccggatgt ttttgcgtca
      961 accccaagga gcacctctcc atgtcgcgta tcgcgaaggg cctggtcctg acctccgccg
     1021 ccgtcgccgc ggtggccggc accgccggag tcgccgccgc cgactccggc gcgcacggcg
     1081 cggccgccca ctccccgggt gtgctgtccg gcaacgccgt ccagattccg atccacatcc
     1141 cgatcaacct ctgcggcaac accgtcgacg tgatcggcct gctgaacccg gccttcggca
     1201 acaactgcct caaccactga ggtcgcgtca cccatagacc accggccgct ctccatgggg
     1261 agggtggccg gtttcgcgtg cgctgacccg attggagggc cgcggcgccg acgcggagca
     1321 cggcgcttga cgaggcccct caccagcggt gatgccctac ggagggcggt acgggcggtt
     1381 gcggagagta tccgtcggtt gcggagcgca atcggtgctt ccacggtgga gacaagcccg
     1441 acgcaatcga aaggaacaac agacatgcgt ggtctgcccg ttcggcgtat cgcctccacc
     1501 gccctctgcg ccaccctcgt tctcggaatc gccggaccgg ccgccatggc ggccgaccac
     1561 gaccccgccc gcgagcgcac ccccgccacc tcccaggcgc cggtcgccga cgcgaaggca
     1621 ctgctgggcc agacgcagac gctgaacgac ctcggtgtcg tgctcgaccc ggtcaccaag
     1681 atgctcgacg ccgtgctcaa ggccgacgag ggccagctct ccgccgagga ggccaagaag
     1741 ctcggcgacg cggcgaaggc gtcgatcgcc aagctcggca cggcggcctc cgcggccccg
     1801 gcggcccccg ccgccccggc cgttcccgcc gtcccggccg ccccctcggc cccgacggtc
     1861 ccctccacgt ccaacgcgcc gatcgtcacg ctccccgccc ccgcctcgct ggtcaagaac
     1921 gacccggcgg cgtccgccac cgacgtcagg gccggtgtgc tcgacgacct gcagaaggcg
     1981 atcgacgcgc tgatcgaggc cgccacctcc ggcctggtcg accaggtcgt cccggccgcc
     2041 acggccgtgg tgaccgggct cgtcaacgta ctgaccacca cgctggtcgg cggcgccctg
     2101 cccgcgcaca ccgtcgccgg cctgcccccg ctgccgtccc tgccggccgc cgaggcctcc
     2161 gcgccgcagt cgccggccgc gctgccggag gagccgtccg ccgccgcgga ggccgcgccc
     2221 gcgcagccgc agtcgccggt cgccctgccg gaggagccgt ccgccgtgtc ggaggcggcg
     2281 cccgcgcagc cggaactgac gccggccctg cccgaggcgc cgcagaccgt gcccacggtc
     2341 ggactgttca tgaccccctg acccttggcc tccaggcccc ctggtccctg tcctcccggg
     2401 gaccccgtct gaccctctct gaccccctct gaacccctct gaccttcagg aaccgagtcg
     2461 tcgtgccggc cgtgcgggcc ggctcgacga ccgttcatat gtcttcctcg cacagggttt
     2521 ccgaccagcg cggcactcgt tgggcacggc gtccggattc cccccggcgg cacgagtcgc
     2581 cgaagaaagg aacacgatga agtccctgaa ggccgccgct gtcgtcgccg ggtccatggt
     2641 catcgcgggt gccgccgcgc ccgcgttcgc ccacgacacc gccgacctca cgcccaccag
     2701 cctcaacggc gccgtcaaca cgctcaccaa ccgccccatc gagccgatgg acgtcatgcc
     2761 gctgaagcag cagtcgaacg ccctcgacac cgagaacaag gactcggtgc tcagcacggt
     2821 gaacggcgtc accaagaccc tcaacgccaa gggcggcctg ctcggcgggc tgccgctgcg
     2881 gggctgacgg acaccccggc ccggcccgcg ggccggcgcc ccagggaccg ggccgattcc
     2941 gccccaccgg gaatcggccc gcttcggtct gttcgagcgg aatccgggac cgtccctcgt
     3001 tcgtgtggag agcacagcgg ccgtcgcccg ctcgggtgag aaggcgtccg ggccggttcc
     3061 ggtacgccga taaggtcgcg cggtgacctc aacccccaac gaaacccgcc ccttccgcgc
     3121 catggacatg ggcaccctcg tcgtcatggc ctggagcggc gaggcccccg gcggcgacat
     3181 gccgtacctc ctcgcctaca ccctcgggga cggccccgac ggccccgagg ccgcctccgc
     3241 cgccgtcgcg cgcatgctga acgacaacgg cctccccgtc ggcggcgacc tcgtcgacgg
     3301 cgcctcgaag cccggcctgc cggtcggcct gctcgtcgag gccggccagg ccgtcgtcaa
     3361 catgccgcac ctcaacgcgc agtgcacccc gcccccggag tggctcgcgg ccgtcggcga
     3421 acgcggcttc gcctacttcg tgttcaccac ccgcccctgg ccggaggccc agcccgggaa
     3481 gccggtcgac cccgaggccc tggccgcgtt cgcgggcgcc gacgagaccc tggacgccgc
     3541 cgcgcacgtg gtcctcccgg tccgcagcct gcgctcctga tggcggccgc cgccggacgg
     3601 cacgcccacc cggcggtgcc ggccgtgggc gggggccgcg gattcgcgct gctgctggtg
     3661 ctcaccgggg cggccgggct gctggcctcc tgggtcatca ccctcgacaa gttcaagctg
     3721 ctggaggacc cggacttcac ccccgcgtgc agcctgaacc cggtggtgtc ctgcggcagc
     3781 gtcatggaga gcgaccaggc ctccgccttc gggttcccca acccgatgct gggcctggcc
     3841 gcctacgccg tcgtcgtctg cgtcggcatg agcctgctgg cgggcgcggc cttcccccgc
     3901 tggtactggt tgaccttcga cgccggctgc ctgttcggcg tcggcttcgt gtcctggctc
     3961 cagttcgagt cgctgtaccg gatcaacgcg ctgtgcctgt ggtgcgccct cgcgtggctc
     4021 gcgaccgtcg tcctgttctg gtacgtcacc tccttcgccg tacgccacgg cttcctgccc
     4081 gcgcccgacg ggctgcgctc gttcctcacc gacttcacct gggtgctgcc gctcgcgcac
     4141 ctcggcgtga tcggcatgct gatcctcacc cgctggtggg acttctggac gagctgactg
     4201 tgcggacgta gtactactgc ggcacccggg ggaattccgc gcgagtgccg cactcgggac
     4261 gcttcccggg ctcgttgggg gtacggacgc ccagcccccg actcctgaaa gggaccgttc
     4321 ctgagatgaa gccgaccacc cgaggaaccc ttgccgccgt cgtcaccggc gtcgcggccg
     4381 ccgtcggagc cgccgcctcc cccgcggtcg cggccgacac ggtccccgtg cccgtgccgc
     4441 tgaacggggc cgagaagtcc ctcggcatgg agctgcccga gatcggcgcc gagctgcccc
     4501 tgccgaagcc gggcagcccc gagggcccgc ggtacgtcga gggccggctc gtcccggacc
     4561 gggcggtccc gcagctgccg gtgcgcggcg ggctgcccgg cgcggacgta cgggcgccgc
     4621 tgccgcgcct cctcggcgac gacttcgacc acgtcggcgt ccacgccccc gcctccgacc
     4681 tgcgcaccct cggccccggc ctggccgtgg acgccccgct caccccgccc aaccccgaca
     4741 actacggact gcccggcctg aagccgccgc aggcgggcgt ggtcacgccg gtgctgcaga
     4801 ccgtgcccgg cgccgagctg ggcacgggac cgggactgta aggacggccc cgcctcgtcg
     4861 aagggccacg ggcccgggag ccgcacaccg gccgcccggg cccttcggct gtccgggggg
     4921 tagcggccac ggacagtgac cgtgggggcg ggcgggcggt tagtcccgta cggacaaccg
     4981 aaccgtacga ggagcacatg atggccgtca gcgtcggcgc agccgcggtg aacatggaga
     5041 acgagccagg gagagcggcg ggaacgcccg acccgccggc gggaacgccg gacccgccag
     5101 cggggcgcgg cacccgccgc gacctggcgc gcggcatgtt cgccgcggcc gtcgccctca
     5161 cgatcgcgcc gctggtcgtg gcgtcccggc cggcccgccg ggaggacccg cagaacccgc
     5221 aggacccgca gaacccgcag gacccgcccg cggacgtcgc cttcgacgag atctaccggg
     5281 gccggcgcat ccgcggcctg cgcgcgcccg gcgcggtccc ggcctccgcc gcgggccgcg
     5341 tcttcggcac ggacgggtgg cacgtcaccg tcgacggcca cccgctgcac ctgatgcgcc
     5401 gcgccgacgg cggctggctg acgatggtcg accactaccg ctcgtacccg acccccctgg
     5461 aagccgcccg cgcggccgtc gacgaactgg gcccgggaca gagcctggga acgccggacg
     5521 cggacacggc gagcggggag ggaacccacg agggaaccca tgaaggaacc cggggacagg
     5581 ggggccacca tggtgtacac ccgtaaggac gtcagcaccc tcaccgccac cgagcgacgg
     5641 cgtttcgtca aggcgctgct ggagctcaaa cgccgtggcg agtacgacga gttcgtgcgc
     5701 acgcacatcg agtactacgt cgccgacggc gagaacggac tgcggacggc gcacatggcg
     5761 ccctccttcc tgccctggca ccgccagttc ctgctggagc tggagaaggc gctgcgccgc
     5821 atcgactcgt cggtgtccgt gccgtactgg gactggaccc gcgaccgcac cacgacctcc
     5881 gtgccgtgga ccaaggacct gctgggcggc aacggacgcc cctccgaccg gcaggtgacg
     5941 accggcccgt tcgcctacgc ggagggtaac tggacgctca aggtcggtgt caccaacggc
     6001 aagttcctga cccgggacct cggccgggcc cgggaccccc tccaactgcc caccgccggc
     6061 gagctgcagt gggcgctgaa ggatcccgtc tacgacgtcg ctccctggga ctcgacggtc
     6121 acccagggct tccgcaacaa actggagggc tggggcaccg gacgcggcag cgcctcctgg
     6181 cgcaaccaca acctggtgca ccggtgggtc ggcggcgcga tggtcagcgg cgcgtccgtc
     6241 aacgacccgg tgttctggct gcaccacgcc ttcgtcgacc tggtgtggtc ccgctggcag
     6301 caacgccacc gggacgaccg gtaccgcccg gtgaaacagc cggccgcggg cgacccccag
     6361 cggggacgcg tcgtggcacg gcacgagaag ctgccgccgt gggacgtgac gccggacgag
     6421 ctggacgatc acgggagcat ctaccggtac gcgtaaccgt gtgcgcgggg gcgggtggcg
     6481 gcccgtggcg gacatgggag agccccggcg ctcggggtgc cggggctcct ctcgggtggg
     6541 gcgtcagtgg ccgtagccgt ggccgccccc gtgacccttg ccgtcgtcgt gacccttgcc
     6601 gtcgtcgtgg cccttgccct tgtggtcgct gtggttgagg caggtgttgc cgaaggccgg
     6661 gttcagcaga ccgatcacgt cgacggtgtt cccgcacagg ttgatcggga tgttcagcgg
     6721 cacctgagcg acgttgcccg acaccgcgcc gggcgagtgg acggccgcgc cctgggcccc
     6781 cgcgtccgcc acggcgagac cggccccgcc aaggaccacg gcgcccgtac cgagggcgac
     6841 tccggctgcc ttcgtgatgc gcgacatcac gttctcctta agactcgttg atcgcgacga
     6901 cagttggagc cgccgcactc cccgttcaac gcgacggatc atgccgggtc acgccgatcc
     6961 gccatggatc acccatcctt ctcagaaggg cacaaacggc ggcgcgcacg cggggagcgc
     7021 atacggggcg cgcaaacgac agcgcatacg aagcgagcgg ggttcgcacg ccctggacgg
     7081 gcactgcgaa cgagacgatg gggcacagga gtggcgtcag gcaggccgag gcacgatgct
     7141 caccgcgcga tagtcgtacc cgtcctgttt gaagccgggg gcttcccacg tgaggtcgac
     7201 ccgctgcccg ggcgacagcg tgcggaaacc gggcgcctgg atgtcggagt agtggccgaa
     7261 acagccgccg ggggtctctg gggagtcgag tacgccccac ccttcctcgt cgtaccactc
     7321 gcggacggtc gcagtcacca tgcgcgggac tctacgggta acaactcggc caagccctct
     7381 cggggtgagg gcggagactg ggcggcgccc cggcgacccg gtgatctccc gcagtgtgtc
     7441 gagtgtcacc ccgccctgag cggcaaaaac gccctcccga ggggagggcg gagactggcg
     7501 cccccggcag gactcgaacc tgcggccaag cgcttagaag gcgcctgctc tatccactga
     7561 gctacggggg ccggtgtggt ggcctctgtc gtggtgcccg ggtgcgggct gatccgtgac
     7621 gttgccgggg acaaggatag ggctcccgga tccttgaccc tgttgcttcg cctccgtggc
     7681 tcaatgtgga ggttcggtga agcggtcctg ataatcgcag gcaggtacga atcgtgcatc
     7741 gcttttgacg catcgcgcac cgggtgttgt gcactcgtta tgcctcagtc acctccgtgt
     7801 cccagtcgtc ccttccgtcc cttgtgtcct gtcggcgcgc agacatgctc atatgcttca
     7861 gaaatcccat aaaattgggc attcttcgca tgtggtgacc ttggacgtac ggcctcagct
     7921 gctcgacgca ctctccacgc tgcgcgaccg cgtcgccgcc gcacgcttcc cgctgcccct
     7981 gtcgggggct ccacgcgcgc gtgccaaccg cgacgaactg ctcgcccagc tcgacgacta
     8041 cttggtgccc cggttgagac agcccgaagc gccggtactg gccgtagtgg gcggctccac
     8101 cggcgccggg aagtcgacac tcgtcaactc gctggtcgga cgtcgggtca gcgaggcggg
     8161 cgtactgcgg ccgacgacgc ggacaccggt gctggtctgc aacccggagg accaccactg
     8221 gttcagcggg atgcgcgtgc tccccgacct cacgcgcgtc tgggtgcccc aacaggagtc
     8281 cggtgacgag ctgctgctcc ccggcgagaa ccccacgcgc gtactgcgca tcgagaccac
     8341 cgagaccctg ccgcccggcc tcgccctcct cgacgcgccc gacatcgact cgctggtcgc
     8401 cgacaaccgc gtcctcgccg ccgagctgat ctgcgccgcc gacatctgga tcatggtcac
     8461 cacggcggcc cggtacgccg acgccgtccc ctggcatctg ctgcgcaccg ccaaggagta
     8521 cgacgtcacg ctggtgaccg tcctcgaccg cgtaccgcac caggtcgtgt ccgaggtctc
     8581 ccggcagtac ggcgccctcc tcaccaaggc cggcctcggc cacgtgcccc ggttcacggt
     8641 gcccgagttg cccgagtcgg ccgggggtgg cgggctgctg ccggtcaccg ccgtcgcacc
     8701 actgcgcacc tggctcattc accaggccca ggaccccgcc gcccggcagc acaccctggc
     8761 ccgcaccgcg cacggcgtcc tgaactccct caagtcccgg atgcccgaac tggccagcgc
     8821 cgccgccgcc cagtacgccg ccgccctccg cctcaccgcc gccgtcgaca ccgcctacga
     8881 cagcgagtac gcgcgcgtgc gcggacggct ccaggcgggg gccgtcctcg ccggggacgc
     8941 cctcaagcgc tggcgggcct tcccgctgga ctgcaccccc aaagagctgc tggacgcgct
     9001 cgtggagagc ctcggcgacc ttctgctgtg cgccgtcacc gcggccgacg agcgcgtcga
     9061 cgacgcctgg cggctcgagc ccgcggccgg cgcccccgag ctggcggacg acgccgaacc
     9121 acaccacgcg gggcaccgga tcgggctggc cgtacggcgg tggcggcggg agctggagga
     9181 gtacgccgag gaggaggtgc gccacttgga acggagcgtc gcgcccgacc ccgagatgat
     9241 cgccgcactc gtcgccaccg tgctgctcgg cgggcggcgc gccaggtcgg cgggggaggg
     9301 gctcgccgag cggatcggcg cacacggggc gctgcggctg cgtgaccggg gcggccggct
     9361 cctcgccgag cacctggatc gcgtgatcca aaccgaacgc gagcgccgac tcgccccgct
     9421 cgacgccctc gacgtccatc ccgaacccca ggccgcactc atcgccgcgc tgtccgtatt
     9481 gcagaaggag aggtgaccgg tggccgccgt cactgaccag gaccacacgg atcacgccga
     9541 tcgtgctcac cgtgccgatc acgtaagtca ctcggctctt gccggtgccc gcatcggtat
     9601 cggcgcctcc gagcgtcaga cggatcacgt cgagcgggcg gatgacaaga cctccgaggt
     9661 ggagaacgac ttgcgtgacg agggtggttc cgagggcacg gacaagccgt ctggggagac
     9721 ggaaaccggt gccgccgggg agcgagggga ggccgcggac tgcgagagca gtgaggaggg
     9781 ggagagggga aagggcacgg agagctcgga gagcgtagag agcgcaggca gcgcagggag
     9841 cgcggagagc tcggaggggg ccggggtcgc cgaggaaccc gcgtcttccg cgccttccga
     9901 ggctgcctcc gctgcccccg gaccggcctc ggagaccggc accgacgagg aagcgcggcg
     9961 tgcgatggcg ggagacggtg accacgcgcg cgtgcggggt gagaagcggg ggcgcgccag
    10021 ggcgcgtgcc aaggccgaca ccagtacgaa aggcaaggtg attgccgacg taagggtgaa
    10081 cggcgagacg aaagtgaccc ctgacgcagg tgtgaaggcc gacaccgtga aggccgacac
    10141 cggcgcgaag gcccgtgccc cgaaggcaga cacaggcgtg atcgccgaca ccgtgaaggc
    10201 cgacccgcgc gggcaagcgc gtgcgcaagc cgacccgcgc ggtcacaccg acccgcgcgc
    10261 cgaagccgac gacagctccg cgcgccgtac cgacgccgag cccgcctggg acgacgggct
    10321 gatcgcgcgg cgggtcaccg aaggggccgc cccggtcgag caggccgtcg tcgtggagac
    10381 gcggagcggc aagtcgcagg gggtgacgcc gctggcgtac gacgggtcgc tgcggtcgcg
    10441 gctggacgcg ctgcgtgagc tggtggggct gtcgcggacc cggctggaca gccggacgct
    10501 ctccgaggcg ggccgggtgc tcgacgaggc cgccgcgcgg cgccgcctct ccgggcagca
    10561 cacggtcgtc gccatcgccg gggccaccgg cagcggcaag tcgcag
//
